SPAC1006.07


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC1006.07 Feature Typeprotein coding
Synonymstif1 Name Description
Producttranslation initiation factor eIF4A (predicted) Product Size392aa, 44.44 kDa
Genomic Location Chromosome I, 5085486-5087031 (1546nt); CDS:5085563-5086741 (1179nt)

Ensembl Gene Location
  1. och1
  2. rgf2
  3. SPAC1006.07
  4. etd1
  5. win1
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0004004ATP-dependent RNA helicase activityISOSGD:S000001767GO_REF:000002440
GO:0003743translation initiation factor activityISOSGD:S000001767GO_REF:000002438
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0002183cytoplasmic translational initiationNASGO_REF:000005140
GO:0006413translational initiationISOSGD:S000001767GO_REF:000002448
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722317
GO:0016281eukaryotic translation initiation factor 4F complexISOSGD:S000001767GO_REF:00000243
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopySPAC1006.07ΔNullPECO:0000005, PECO:0000137PMID:236978061309
MicroscopySPAC1006.07ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000313inviable after spore germination, without cell division, with abnormal germ tube morphologyMicroscopySPAC1006.07ΔNullPECO:0000005, PECO:0000137PMID:23697806144
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
150854865087031

UTRs

Region Start End Reference
five_prime_UTR50854865085562PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00271 Pfam IPR001650 Helicase, C-terminal 277 353 68
PF00270 Pfam IPR011545 DNA/RNA helicase, DEAD/DEAH box type, N-terminal 44 208 43
SM00487 SMART IPR014001 Helicase, superfamily 1/2, ATP-binding domain 38 235 70
SM00490 SMART IPR001650 Helicase, C-terminal 272 353 67
PS51192 Prosite Profiles IPR014001 Helicase, superfamily 1/2, ATP-binding domain 50 220 69
PS51195 Prosite Profiles IPR014014 RNA helicase, DEAD-box type, Q motif 19 47 23
PS51194 Prosite Profiles IPR001650 Helicase, C-terminal 231 392 69
PS00039 Prosite Patterns IPR000629 RNA helicase, ATP-dependent, DEAD-box, conserved site 166 174 19
PTHR24031 HMMPANTHER 1 392 26
G3DSA:3.40.50.300 Gene3D 226 389 279
G3DSA:3.40.50.300 Gene3D 8 225 279
SSF52540 SuperFamily IPR027417 87 375 294
Low complexity (SEG) seg 345 356

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000174DEAD/DEAH box helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000174

Protein Properties

Ave. residue weight 113.36 Da
Charge -14.50
Isoelectric point 4.62
Molecular weight 44.44 kDa
Number of residues 392
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:182575171192
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
154934.46during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
53576.62during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
110during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
14during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
cancercancer_census141
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
cwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
paa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
far8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
External References
Database Identifier Description
NBRP SPAC1006.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1006.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1006.07 BioGRID Interaction Datasets
Expression Viewer SPAC1006.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1006.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1006.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1006.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1006.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1006.07 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1006.07 Cell Cycle Data
GEO SPAC1006.07 GEO profiles
PInt SPAC1006.07 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542948translation initiation factor eIF4A
EntrezGene2542948translation initiation factor eIF4A
SPD / RIKEN32/32F01Orfeome Localization Data
UniProtKB/SwissProtP47943ATP-dependent RNA helicase eIF4A
ModBaseP47943Database of comparative protein structure models
StringP47943Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594854translation initiation factor eIF4A
RefSeq mRNANM_001020283972h- translation initiation factor eIF4A (SPAC1006.07), mRNA
European Nucleotide ArchiveAAB61679ENA Protein Mapping
European Nucleotide ArchiveCAA56772ENA Protein Mapping
European Nucleotide ArchiveCAB60237ENA Protein Mapping
UniParcUPI000012D369UniProt Archive

Literature for SPAC1006.07

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014