mug134 (SPAC10F6.16)

Gene Standard Namemug134 Characterisation Statuspublished
Systematic IDSPAC10F6.16 Feature Typeprotein coding
Synonymsigo1 Name DescriptionMeiotically Upregulated Gene
Productendosulfine Igo1 Product Size139aa, 15.44 kDa
Genomic Location Chromosome I, 1238896-1240185 (1290nt); CDS:1238896-1239424 (529nt)

Ensembl Gene Location
GO Biological Process
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencyS64A (S64A)276
decreased number of cells with 1C DNA contentmug134Δ1
S64A (S64A)
increased cell population growth ratemug134Δ256
normal cell population growth at high temperaturemug134Δ77
normal growth on glucose carbon sourcemug134Δ318
sensitive to 5-fluorouracilmug134Δ307
viable vegetative cell populationmug134Δ3862

Cell Phenotype

Term NameGenotypesCount
abnormal cellular response to nitrogen starvationS64A (S64A)47
abolished cell cycle arrest in mitotic G1 phase in response to nitrogen starvationS64A (S64A)17
elongated cell during nitrogen starvationS64A (S64A)25
viable vegetative cell with normal cell morphologymug134Δ3103
Target Of
FYPO affected by mutation in ppk18 greatwall kinase Ppk18
FYPO affected by mutation in sck1 serine/threonine protein kinase Sck1
FYPO affected by mutation in sck2 serine/threonine protein kinase Sck2
FYPO affected by mutation in tor2 serine/threonine protein kinase Tor2
GO substrate of ppk18 greatwall kinase Ppk18
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1238896..1238979, 1239025..1239108, 1239173..1240185
Intron1238980..1239024, 1239109..1239172
CDS1238896..1238979, 1239025..1239108, 1239173..1239424
3' UTR1239425..1240185SPAB1289
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04667 Pfam IPR006760 Endosulphine 26 97 1
PTHR10358 HMMPANTHER 16 139 1
PTHR10358:SF6 HMMPANTHER 16 139 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.09 Da
Charge 8.50
Codon Adaptation Index 0.36
Isoelectric point 10.33
Molecular weight 15.44 kDa
Number of residues 139

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2293
added by ppk18, added during cellular response to nitrogen starvationS64
added during cellular response to rapamycinS64
present during mitotic M phaseS31
present during mitotic M phaseS99
present during mitotic M phaseS64
present during mitotic M phaseS89
present during mitotic M phaseS102
in presence of torin 1S64
S31, S64, S78, S99, S102, S108, S115, S128
level fluctuates during mitotic cell cycleS64
Annotation ExtensionEvidenceResidueReference
added by ppk18
added during cellular response to nitrogen starvation
IDA S64 PMID:26776736
added during cellular response to rapamycin IDA S64 PMID:26776736
present during mitotic M phase experimental evidence S31 PMID:21712547
present during mitotic M phase experimental evidence S99 PMID:21712547
present during mitotic M phase experimental evidence S64 PMID:21712547
present during mitotic M phase experimental evidence S89 PMID:21712547
present during mitotic M phase experimental evidence S102 PMID:21712547
in presence of torin 1 IDA S64 PMID:26776736
IDA S115 PMID:25720772
IDA S128 PMID:25720772
IDA S108 PMID:25720772
IDA S78 PMID:25720772
IDA S99 PMID:25720772
IDA S31 PMID:25720772
level fluctuates during mitotic cell cycle experimental evidence S64 PMID:24763107
IDA S64 PMID:25720772
experimental evidence S31 PMID:24763107
IDA S102 PMID:25720772
O-phospho-L-threonine 1087
present during mitotic M phaseT92
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T92 PMID:21712547
O4'-phospho-L-tyrosineY61 281
Annotation ExtensionEvidenceResidueReference
IDA Y61 PMID:25720772
mannosylated residue 89
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence PMID:26644575
O-phosphorylated residueS99,S102, S99,S108, S102,S108, S102,T111, S115,S120,S124, S115,S123,T130, S115,S124,S128, S118,S123, S120,S123,S128, S120,S123,T130, S120,S128, S124,S128, S128,S136 2461
Annotation ExtensionEvidenceResidueReference
IDA S115,S123,T130 PMID:25720772
IDA S120,S123,S128 PMID:25720772
IDA S99,S102 PMID:25720772
IDA S118,S123 PMID:25720772
IDA S115,S120,S124 PMID:25720772
IDA S120,S123,T130 PMID:25720772
IDA S120,S128 PMID:25720772
IDA S102,T111 PMID:25720772
IDA S128,S136 PMID:25720772
IDA S115,S124,S128 PMID:25720772
IDA S124,S128 PMID:25720772
IDA S99,S108 PMID:25720772
IDA S102,S108 PMID:25720772
Gene Expression

Qualitative Gene Expression

protein levelpresent during GO:0072690Western blot evidencePMID:26776736
increased during GO:0006995Western blot evidencePMID:26776736
RNA levelincreased during GO:0051321Northern assay evidencePMID:16303567

Quantitative Gene Expression

View graphical display of gene expression data for mug134 (SPAC10F6.16)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9229.33during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
7280.95during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.7during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
0.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in metazoa3498
conserved in vertebrates3473
conserved in eukaryotes only2525

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC10F6.16 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescued bypom1DYRK family protein kinase Pom1 Phenotypic SuppressionPMID:26776736
rescuesppk18greatwall kinase Ppk18 Phenotypic SuppressionPMID:26776736
rescueswee1M phase inhibitor protein kinase Wee1 Phenotypic SuppressionPMID:26776736
rescuestor2serine/threonine protein kinase Tor2 Phenotypic SuppressionPMID:26776736
enhances phenotype ofcdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic EnhancementPMID:26776736
phenotype enhanced bypyp2tyrosine phosphatase Pyp2 Phenotypic EnhancementPMID:26776736
phenotype enhanced bycdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Phenotypic EnhancementPMID:26776736
phenotype enhanced bysty1MAP kinase Sty1 Phenotypic EnhancementPMID:26776736
External References
Database Identifier Description
NBRP SPAC10F6.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC10F6.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC10F6.16 BioGRID Interaction Datasets
Expression Viewer SPAC10F6.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC10F6.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC10F6.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC10F6.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC10F6.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC10F6.16 Transcriptome Viewer (Bähler Lab)
GEO SPAC10F6.16 GEO profiles
PInt SPAC10F6.16 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC10F6.16 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC10F6.16 Fission yeast phenotypic data & analysis
Cyclebase SPAC10F6.16.1 Cell Cycle Data
SPD / RIKEN05/05D01Orfeome Localization Data
UniProtKB/SwissProtP79058mRNA stability protein mug134
ModBaseP79058Database of comparative protein structure models
STRINGP79058Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593267mRNA stability protein Igo1 (predicted)
RefSeq mRNANM_001018664972h- mRNA stability protein Igo1 (predicted) (mug134), mRNA
European Nucleotide ArchiveAB001289ENA EMBL mapping
European Nucleotide ArchiveCAA15729.1ENA Protein Mapping
UniParcUPI000006C8CCUniProt Archive

Literature for mug134

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017