fhl1 (SPAC1142.08)


Gene Standard Namefhl1 Characterisation Statuspublished
Systematic IDSPAC1142.08 Feature Typeprotein coding
SynonymsSPAC8C9.01 Name Description
Productforkhead transcription factor Fhl1 Product Size743aa, 81.13 kDa
Genomic Location Chromosome I, 3639860-3642338 (2479nt); CDS:3639988-3642219 (2232nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
regulation of transcription from RNA polymerase II promoter288
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth on glucose carbon sourcefhl1+ (wild type)Overexpression258
decreased conjugation frequencyfhl1ΔNull44
resistance to methyl methanesulfonatefhl1ΔNull2
sensitive to 5-fluorouracilfhl1ΔNull271
sensitive to bortezomibfhl1ΔNull256
slow vegetative cell population growthfhl1ΔNull328
viable vegetative cell populationfhl1ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
abnormal septationfhl1+ (wild type)Overexpression151
decreased RNA level during nitrogen starvation37
affecting ste11fhl1ΔNull
elongated multiseptate cellfhl1ΔNull55
normal cell cycle arrest in mitotic G1 phase during nitrogen starvationfhl1ΔNull16
normal sporulationfhl1ΔNull52
viable elongated vegetative cellfhl1+ (wild type)Overexpression301
fhl1ΔNull
viable vegetative cell with normal cell morphologyfhl1ΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3639860..3642338
mRNA3639860..3642338
5' UTR3639860..3639987PMID:20118936
5' UTR3639860..3639987PMID:18641648
CDS3639988..3642219
3' UTR3642220..3642338PMID:20118936
3' UTR3642220..3642338PMID:18641648
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00250 Pfam IPR001766 Transcription factor, fork head 291 384 4
PF00498 Pfam IPR000253 Forkhead-associated (FHA) domain 39 111 8
SM00339 SMART IPR001766 Transcription factor, fork head 289 379 4
SM00240 SMART IPR000253 Forkhead-associated (FHA) domain 38 94 6
PS50006 Prosite Profiles IPR000253 Forkhead-associated (FHA) domain 39 94 8
PS50039 Prosite Profiles IPR001766 Transcription factor, fork head 291 385 4
PTHR11829 HMMPANTHER 1 407 4
PTHR11829:SF124 HMMPANTHER 1 407 1
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 286 376 46
2.60.200.20 Gene3D IPR000253 Forkhead-associated (FHA) domain 32 127 8
SSF46785 SuperFamily 290 384 49
SSF49879 SuperFamily IPR008984 SMAD/FHA domain 28 125 8
PR00053 PRINTS IPR001766 Transcription factor, fork head 312 329 4
PR00053 PRINTS IPR001766 Transcription factor, fork head 335 352 4
PR00053 PRINTS IPR001766 Transcription factor, fork head 291 304 4

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0013111forkhead domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0013111

Protein Properties

Ave. residue weight 109.19 Da
Charge 16.00
Isoelectric point 9.35
Molecular weight 81.13 kDa
Number of residues 743
Sequence
Gene Expression

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.1during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4603
conserved in eukaryotes4514
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withabc4glutathione S-conjugate-exporting ATPase Abc4 Negative GeneticPMID:22681890
negative genetic interaction withago1argonaute Negative GeneticPMID:22681890
positive genetic interaction withalg6glucosyltransferase Alg6 Positive GeneticPMID:22681890
positive genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
negative genetic interaction witharf6ADP-ribosylation factor, Arf family Arf6 Negative GeneticPMID:22681890
synthetic growth defect withase1antiparallel microtubule cross-linking factor Ase1 Synthetic Growth DefectPMID:18931302
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
negative genetic interaction withatp15F0-ATPase epsilon subunit (predicted) Negative GeneticPMID:22681890
negative genetic interaction withbtf3nascent polypeptide-associated complex beta subunit Negative GeneticPMID:22681890
negative genetic interaction withcdc37Hsp90 co-chaperone Cdc37 Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:22681890
positive genetic interaction withelp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
enhances phenotype offkh2forkhead transcription factor Fkh2 Phenotypic EnhancementPMID:18059475
negative genetic interaction withftp105Ubp5 interacting protein Ftp105 Negative GeneticPMID:22681890
negative genetic interaction withfus1formin Fus1 Negative GeneticPMID:22681890
negative genetic interaction withhat1histone acetyltransferase Hat1 Negative GeneticPMID:22681890
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
positive genetic interaction withlsc1Lsk1 associated cyclin Positive GeneticPMID:22681890
positive genetic interaction withlsk1P-TEFb-associated cyclin-dependent protein kinase Lsk1 Positive GeneticPMID:22681890
negative genetic interaction withmed13mediator complex subunit Srb9 Negative GeneticPMID:22681890
negative genetic interaction withmeu10GPI anchored cell surface protein involved in ascospore wall assembly Meu10 Negative GeneticPMID:22681890
negative genetic interaction withmfs2MFS family transmembrane transporter Mfs2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:22681890
synthetic growth defect withmkh1MEK kinase (MEKK) Mkh1 Synthetic Growth DefectPMID:18931302
synthetic growth defect withmph1dual specificity protein kinase Mph1 Synthetic Growth DefectPMID:18931302
negative genetic interaction withnro1negative regulator of Ofd1, Nro1 Negative GeneticPMID:22681890
negative genetic interaction withotu2ubiquitin specific cysteine protease, OTU family, Otu2 Negative GeneticPMID:22681890
positive genetic interaction withpac10prefoldin subunit 3 Pac10 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withpmc2mediator complex subunit Pmc2/Med1 Negative GeneticPMID:22681890
synthetic growth defect withpnk1DNA kinase/phosphatase Pnk1 Synthetic Growth DefectPMID:18931302
negative genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:22681890
negative genetic interaction withpof7F-box protein Pof7 Negative GeneticPMID:22681890
negative genetic interaction withppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withpst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
negative genetic interaction withraf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
synthetic growth defect withrfp1SUMO-targeted ubiquitin-protein ligase subunit Rfp1 Synthetic Growth DefectPMID:18931302
synthetic growth defect withrik1silencing protein Rik1 Synthetic Growth DefectPMID:18931302
negative genetic interaction withrmt3type I ribosomal protein arginine N-methyltransferase Rmt3 Negative GeneticPMID:22681890
negative genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrpl170260S ribosomal protein L17 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrps170240S ribosomal protein S17 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrps2340S ribosomal protein S23 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrps40140S ribosomal protein S4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrps40240S ribosomal protein S4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrps80140S ribosomal protein S8 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsat1Golgi membrane exchange factor subunit Sat1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
positive genetic interaction withsft1SNARE Sft1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsgf73SAGA complex subunit Sgf73 Positive GeneticPMID:25076038
positive genetic interaction withshf1small histone ubiquitination factor Shf1 Positive GeneticPMID:22681890
synthetic growth defect withshk2PAK-related kinase Shk2 Synthetic Growth DefectPMID:18931302
negative genetic interaction withski2Ski complex RNA helicase Ski2 (predicted) Negative GeneticPMID:22681890
synthetic growth defect withslm9hira protein Slm9 Synthetic Growth DefectPMID:18931302
negative genetic interaction withsnu66U4/U6 x U5 tri-snRNP complex subunit Snu66 Negative GeneticPMID:22681890
negative genetic interaction withSPAC2H10.01transcription factor, zf-fungal binuclear cluster type (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC323.03cSchizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withSPAC56F8.02AMP binding enzyme (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC6G9.14RNA-binding protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC9.07cGTPase Rbg1 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withSPBC17A3.06phosphoprotein phosphatase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPBC365.16conserved protein Negative GeneticPMID:22681890
negative genetic interaction withSPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC725.01aspartate aminotransferase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC839.02arrestin Aly1 related, implicated in endocytosis Negative GeneticPMID:22681890
negative genetic interaction withSPBPB10D8.02carylsulfatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBPB7E8.01Schizosaccharomyces specific protein, predicted GPI anchor Negative GeneticPMID:22681890
positive genetic interaction withSPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPCC16C4.106-phosphogluconolactonase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC663.15cconserved fungal protein Negative GeneticPMID:22681890
negative genetic interaction withspf38U5 snRNP complex subunit Spf38 Negative GeneticPMID:22681890
negative genetic interaction withspn1mitotic septin Spn1 Negative GeneticPMID:22681890
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:22681890
negative genetic interaction withssp2AMP-activated protein serine/threonine kinase alpha subunit Ssp2 Negative GeneticPMID:22681890
negative genetic interaction withstm1G-protein coupled receptor Stm1 Negative GeneticPMID:22681890
positive genetic interaction withswd1Set1C complex subunit Swd1 Positive GeneticPMID:22681890
negative genetic interaction withuch1ubiquitin C-terminal hydrolase Uch1 Negative GeneticPMID:22681890
positive genetic interaction withuri1unconventional prefoldin chaperone involved protein complex assembly Uri1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withvps1dynamin family protein Vps1 Negative GeneticPMID:22681890
synthetic growth defect withyam8stretch-activated calcium ion channel Yam8 Synthetic Growth DefectPMID:18931302
positive genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Positive GeneticPMID:22681890
negative genetic interaction withzfs1CCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC1142.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1142.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1142.08 BioGRID Interaction Datasets
Expression Viewer SPAC1142.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1142.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1142.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1142.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1142.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1142.08 Transcriptome Viewer (Bähler Lab)
GEO SPAC1142.08 GEO profiles
PInt SPAC1142.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1142.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1142.08 Fission yeast phenotypic data & analysis
Cyclebase SPAC1142.08.1 Cell Cycle Data
SPD / RIKEN38/38A12Orfeome Localization Data
UniProtKB/SwissProtO14270Fork head transcription factor 1
ModBaseO14270Database of comparative protein structure models
STRINGO14270Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594272fork head transcription factor Fhl1
RefSeq mRNANM_001019695972h- fork head transcription factor Fhl1 (fhl1), mRNA
European Nucleotide ArchiveCAB77015.1ENA Protein Mapping
UniParcUPI000013B77EUniProt Archive

Literature for fhl1

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015