nse6 (SPAC11E3.08c)


Gene Standard Namense6 Characterisation Statuspublished
Systematic IDSPAC11E3.08c Feature Typeprotein coding
Synonyms Name Description
ProductSmc5-6 complex non-SMC subunit Nse6 Product Size522aa, 59.90 kDa
Genomic Location Chromosome I, 5297794-5296049 (1746nt); CDS:5297733-5296165 (1569nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding856
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular response to DNA damage stimulus208
Annotation ExtensionEvidenceWith/FromReference
DNA recombination116
Annotation ExtensionEvidenceWith/FromReference
replication fork protection6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nucleus2695
Annotation ExtensionEvidenceWith/FromReference
Smc5-Smc6 complex8
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to 4-nitroquinoline N-oxidense6ΔNull159
sensitive to camptothecinnse6ΔNull224
sensitive to hydroxyureanse6ΔNull534
sensitive to ionizing radiationnse6ΔNull58
sensitive to methyl methanesulfonatense6ΔNull237
sensitive to UV during vegetative growthnse6ΔNull141
viable vegetative cell populationnse6ΔNull3795
unnamed (unrecorded)

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclense6ΔNull846
cut during cellular response to hydroxyureanse6ΔNull44
elongated vegetative cellunnamed (unrecorded)760
viable elongated vegetative cellnse6ΔNull289
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons5297794..5296049
mRNA5297794..5296049
5' UTR5297794..5297734SPAJ2494
5' UTR5297794..5297734AJ002494
CDS5297733..5296165
3' UTR5296164..5296049SPAJ2494
3' UTR5296164..5296049AJ002494
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08691 Pfam IPR014803 DNA repair protein Nse5/Nse6 1 474 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.75 Da
Charge 5.50
Isoelectric point 7.91
Molecular weight 59.90 kDa
Number of residues 522
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS26
present during mitotic M phaseS29
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S26 PMID:21712547
present during mitotic M phase experimental evidence S29 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.46during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.14during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Subunit Composition
DescriptionQualifierReferenceCount
homomeric(2) PMID:1647898449
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only620
conserved in fungi4604
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesnse1Smc5-6 complex ubiquitin-protein ligase E3 subunit 1 Affinity Capture-MSPMID:16478984
affinity capturesnse1Smc5-6 complex ubiquitin-protein ligase E3 subunit 1 Affinity Capture-WesternPMID:16478984
affinity capturesnse2Smc5-6 complex non-SMC subunit 2, SUMO ligase Affinity Capture-MSPMID:16478984
affinity capturesnse2Smc5-6 complex non-SMC subunit 2, SUMO ligase Affinity Capture-WesternPMID:16478984
affinity capturesnse3Smc5-6 complex non-SMC subunit Nse3 Affinity Capture-MSPMID:16478984
affinity captured bynse4Smc5-6 complex non-SMC subunit Nse4 Affinity Capture-MSPMID:16478984
affinity captured bynse5Smc5-6 complex non-SMC subunit Nse5 Affinity Capture-MSPMID:16478984
affinity captured bynse5Smc5-6 complex non-SMC subunit Nse5 Affinity Capture-WesternPMID:16478984
forms complex withnse5Smc5-6 complex non-SMC subunit Nse5 Reconstituted ComplexPMID:16478984
affinity capturesnse5Smc5-6 complex non-SMC subunit Nse5 Affinity Capture-MSPMID:16478984
binds DNA-binding domain construct withnse5Smc5-6 complex non-SMC subunit Nse5 Two-hybridPMID:17762865
binds activation domain construct withnse5Smc5-6 complex non-SMC subunit Nse5 Two-hybridPMID:23695164
affinity capturessmc5Smc5-6 complex SMC subunit Smc5 Affinity Capture-MSPMID:16478984
forms complex withsmc5Smc5-6 complex SMC subunit Smc5 Reconstituted ComplexPMID:16478984
affinity captured bysmc5Smc5-6 complex SMC subunit Smc5 Affinity Capture-MSPMID:16478984
forms complex withsmc5Smc5-6 complex SMC subunit Smc5 Reconstituted ComplexPMID:17005570
affinity capturessmc6Smc5-6 complex SMC subunit Smc6 Affinity Capture-MSPMID:16478984
forms complex withsmc6Smc5-6 complex SMC subunit Smc6 Reconstituted ComplexPMID:16478984
affinity capturessmc6Smc5-6 complex SMC subunit Smc6 Affinity Capture-WesternPMID:16478984
forms complex withsmc6Smc5-6 complex SMC subunit Smc6 Reconstituted ComplexPMID:17005570
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
positive genetic interaction withamk2AMP-activated protein kinase beta subunit Amk2 Positive GeneticPMID:22681890
positive genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
negative genetic interaction withapn2AP-endonuclease Apn2 Negative GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
negative genetic interaction withbag101BAG family molecular chaperone regulator Bag101 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
positive genetic interaction withcgi121EKC/KEOPS complex subunit Cgi121 (predicted) Positive GeneticPMID:22681890
synthetic growth defect withchk1Chk1 protein kinase Synthetic Growth DefectPMID:16478984
negative genetic interaction withckb2CK2 family regulatory subunit Ckb2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withclr3histone deacetylase (class II) Clr3 Negative GeneticPMID:22681890
positive genetic interaction withcpd1tRNA (m1A) methyltransferase complex catalytic subunit Cpd1 Positive GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withcph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
synthetic growth defect withcsn1COP9/signalosome complex subunit Csn1 Synthetic Growth DefectPMID:18931302
negative genetic interaction withcsn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:22681890
negative genetic interaction withcsn2COP9/signalosome complex subunit Csn2 Negative GeneticPMID:22681890
negative genetic interaction withcsn3COP9/signalosome complex subunit Csn3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcsn4COP9/signalosome complex subunit Csn4 Negative GeneticPMID:22681890
negative genetic interaction withcsn5COP9/signalosome complex subunit Csn5 Negative GeneticPMID:22681890
synthetic growth defect withcsn71COP9/signalosome complex subunit 7a (predicted) Synthetic Growth DefectPMID:18931302
synthetic growth defect withctf18RFC-like complex subunit Ctf18 Synthetic Growth DefectPMID:18931302
negative genetic interaction withcwf19complexed with Cdc5 protein Cwf19 Negative GeneticPMID:22681890
negative genetic interaction withdad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
negative genetic interaction withdam1DASH complex subunit Dam1 Negative GeneticPMID:22681890
negative genetic interaction withdbl8tRNA-splicing endonuclease positive effector Dbl8 Negative GeneticPMID:22681890
negative genetic interaction withdbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
synthetic growth defect withdcc1Ctf18 RFC-like complex subunit Dcc1 Synthetic Growth DefectPMID:18931302
negative genetic interaction withdcc1Ctf18 RFC-like complex subunit Dcc1 Negative GeneticPMID:22681890
synthetic growth defect withdis2serine/threonine protein phosphatase PP1 subfamily, Dis2 Synthetic Growth DefectPMID:21965289
synthetically rescued bydmc1RecA family ATPase Dmc1 Synthetic RescuePMID:22855558
positive genetic interaction withdmc1RecA family ATPase Dmc1 Positive GeneticPMID:22681890
positive genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Positive GeneticPMID:21504829
negative genetic interaction withduo1DASH complex subunit Duo1 Negative GeneticPMID:22681890
negative genetic interaction witheif21translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
positive genetic interaction withelp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
synthetic growth defect withfbh1DNA helicase I, ubiquitin ligase F-box adaptor Fbh1 Synthetic Growth DefectPMID:18931302
negative genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
negative genetic interaction withfkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhif2Set3 complex subunit Hif2 Negative GeneticPMID:22681890
positive genetic interaction withhip1hira protein, histone chaperone Hip1 Positive GeneticPMID:22681890
synthetic growth defect withhrq1RecQ type DNA helicase Hrq1 (predicted) Synthetic Growth DefectPMID:22064477
negative genetic interaction withhrq1RecQ type DNA helicase Hrq1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withklp6kinesin-like protein Klp6 Negative GeneticPMID:22681890
synthetic growth defect withmcs4response regulator Mcs4 Synthetic Growth DefectPMID:18931302
negative genetic interaction withmph1dual specificity protein kinase Mph1 Negative GeneticPMID:22681890
synthetic growth defect withmrc1mediator of replication checkpoint 1 Synthetic Growth DefectPMID:18931302
negative genetic interaction withmug154conserved fungal protein Negative GeneticPMID:22681890
positive genetic interaction withmug80cyclin Clg1 (predicted) Positive GeneticPMID:22681890
synthetic lethal withmus81Holliday junction resolvase subunit Mus81 Synthetic LethalityPMID:22855558
synthetic lethal withmus81Holliday junction resolvase subunit Mus81 Synthetic LethalityPMID:16478984
negative genetic interaction withnap1nucleosome assembly protein Nap1 Negative GeneticPMID:22681890
synthetic lethal withnse2Smc5-6 complex non-SMC subunit 2, SUMO ligase Synthetic LethalityPMID:16478984
synthetic lethal withnse3Smc5-6 complex non-SMC subunit Nse3 Synthetic LethalityPMID:16478984
negative genetic interaction withnsk1Clp1-interacting, microtubule plus-end binding Nsk1 Negative GeneticPMID:22681890
negative genetic interaction withnth1DNA endonuclease III Negative GeneticPMID:22681890
negative genetic interaction withnto1histone acetyltransferase complex subunit Nto1 (predicted) Negative GeneticPMID:22681890
rescued by overexpression ofnts1Clr6 histone deacetylase complex subunit Nts1 Dosage RescuePMID:25002536
negative genetic interaction withnup132nucleoporin Nup132 Negative GeneticPMID:22681890
negative genetic interaction withnup40nucleoporin Nup40 Negative GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
synthetic growth defect withpht1histone H2A variant H2A.Z, Pht1 Synthetic Growth DefectPMID:18931302
negative genetic interaction withpht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
synthetic growth defect withpku80Ku domain protein Pku80 Synthetic Growth DefectPMID:18931302
negative genetic interaction withpku80Ku domain protein Pku80 Negative GeneticPMID:22681890
synthetic growth defect withpli1SUMO E3 ligase Pli1 Synthetic Growth DefectPMID:18931302
negative genetic interaction withpli1SUMO E3 ligase Pli1 Negative GeneticPMID:22681890
synthetic growth defect withpnk1DNA kinase/phosphatase Pnk1 Synthetic Growth DefectPMID:18931302
negative genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:22681890
negative genetic interaction withpob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
synthetic growth defect withpof8F-box protein Pof8 Synthetic Growth DefectPMID:18931302
negative genetic interaction withpom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
positive genetic interaction withppa2serine/threonine protein phosphatase Ppa2 Positive GeneticPMID:22681890
positive genetic interaction withprp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Positive GeneticPMID:22681890
synthetic growth defect withpub3HECT-type ubiquitin-protein ligase E3 Pub3 (predicted) Synthetic Growth DefectPMID:18931302
negative genetic interaction withpzh1serine/threonine protein phosphatase Pzh1 Negative GeneticPMID:22681890
synthetic growth defect withrad13DNA repair nuclease Rad13 Synthetic Growth DefectPMID:18931302
synthetic growth defect withrad13DNA repair nuclease Rad13 Synthetic Growth DefectPMID:16478984
synthetic growth defect withrad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Synthetic Growth DefectPMID:18931302
negative genetic interaction withrad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Negative GeneticPMID:22681890
synthetic growth defect withrad3ATR checkpoint kinase Rad3 Synthetic Growth DefectPMID:18931302
synthetic lethal withrad3ATR checkpoint kinase Rad3 Synthetic LethalityPMID:16478984
negative genetic interaction withrad3ATR checkpoint kinase Rad3 Negative GeneticPMID:22681890
synthetically rescued byrad51RecA family recombinase Rad51/Rhp51 Synthetic RescuePMID:22855558
synthetically rescued byrad51RecA family recombinase Rad51/Rhp51 Synthetic RescuePMID:16478984
positive genetic interaction withrad51RecA family recombinase Rad51/Rhp51 Positive GeneticPMID:22681890
synthetic growth defect withrad55RecA family ATPase Rad55/Rhp55 Synthetic Growth DefectPMID:16478984
synthetically rescued byrad55RecA family ATPase Rad55/Rhp55 Synthetic RescuePMID:16478984
synthetic lethal withrad60DNA repair protein Rad60 Synthetic LethalityPMID:16478984
negative genetic interaction withraf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Negative GeneticPMID:22681890
negative genetic interaction withraf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
synthetically rescued byrec12meiotic recombination endonuclease Rec12 Synthetic RescuePMID:22855558
synthetic growth defect withres2MBF transcription factor complex subunit Res2 Synthetic Growth DefectPMID:18931302
negative genetic interaction withres2MBF transcription factor complex subunit Res2 Negative GeneticPMID:22681890
synthetic growth defect withrhp14XP-A family homolog Rhp14 Synthetic Growth DefectPMID:18931302
negative genetic interaction withrhp14XP-A family homolog Rhp14 Negative GeneticPMID:22681890
synthetic growth defect withrhp18Rad18 homolog ubiquitin protein ligase E3, Rhp18 Synthetic Growth DefectPMID:18931302
synthetic growth defect withrhp18Rad18 homolog ubiquitin protein ligase E3, Rhp18 Synthetic Growth DefectPMID:16478984
negative genetic interaction withrhp7Rad7 homolog Rhp7 Negative GeneticPMID:22681890
positive genetic interaction withrox3mediator complex subunit Med19/Rox3 Positive GeneticPMID:22681890
positive genetic interaction withrpb7DNA-directed RNA polymerase complex II subunit Rpb7 Positive GeneticPMID:22681890
positive genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:22681890
synthetic lethal withrqh1RecQ type DNA helicase Rqh1 Synthetic LethalityPMID:16478984
negative genetic interaction withrsv2transcription factor Rsv2 Negative GeneticPMID:22681890
negative genetic interaction withrtt109RTT109 family histone lysine acetyltransferase Negative GeneticPMID:22681890
negative genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
positive genetic interaction withsfp1transcription factor Sfp1 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withsnt2Lid2 complex subunit Snt2 Negative GeneticPMID:22681890
synthetic growth defect withSPAC12B10.15cribonuclease H2 complex subunit (predicted) Synthetic Growth DefectPMID:18931302
negative genetic interaction withSPBC1861.07elongin C (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC36B7.08cnucleosome assembly protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withspc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
positive genetic interaction withSPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPCC16C4.106-phosphogluconolactonase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
negative genetic interaction withsrp1SR family protein, human SRFS2 ortholog Srp1 Negative GeneticPMID:22681890
synthetic growth defect withsrs2ATP-dependent DNA helicase, UvrD subfamily Synthetic Growth DefectPMID:18931302
negative genetic interaction withsrs2ATP-dependent DNA helicase, UvrD subfamily Negative GeneticPMID:22681890
positive genetic interaction withssp2AMP-activated protein serine/threonine kinase alpha subunit Ssp2 Positive GeneticPMID:22681890
negative genetic interaction withssu72phosphoric ester hydrolase Ssu72 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withste20Rictor homolog, Ste20 Positive GeneticPMID:22681890
synthetic growth defect withswi10DNA repair endonuclease non-catalytic subunit Swi10 Synthetic Growth DefectPMID:18931302
synthetic growth defect withswi3replication fork protection complex subunit Swi3 Synthetic Growth DefectPMID:18931302
negative genetic interaction withswi3replication fork protection complex subunit Swi3 Negative GeneticPMID:22681890
synthetically rescued byswi5Swi5 protein Synthetic RescuePMID:16478984
synthetic growth defect withtdp1tyrosyl-DNA phosphodiesterase Tdp1 Synthetic Growth DefectPMID:18931302
negative genetic interaction withtip1CLIP170 family protein Tip1 Negative GeneticPMID:22681890
negative genetic interaction withtrm10tRNA m(1)G methyltransferase Trm10 Negative GeneticPMID:22681890
negative genetic interaction withtrt1telomerase reverse transcriptase 1 protein Trt1 Negative GeneticPMID:22681890
negative genetic interaction withusp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
synthetic growth defect withuve1endonuclease Uve1 Synthetic Growth DefectPMID:16478984
synthetic growth defect withyam8stretch-activated calcium channel Yam8 Synthetic Growth DefectPMID:18931302
External References
Database Identifier Description
NBRP SPAC11E3.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC11E3.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC11E3.08c BioGRID Interaction Datasets
Expression Viewer SPAC11E3.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC11E3.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC11E3.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC11E3.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC11E3.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC11E3.08c Transcriptome Viewer (Bähler Lab)
GEO SPAC11E3.08c GEO profiles
PInt SPAC11E3.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC11E3.08c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC11E3.08c Fission yeast phenotypic data & analysis
Cyclebase SPAC11E3.08c.1 Cell Cycle Data
SPD / RIKEN52/52B04Orfeome Localization Data
UniProtKB/SwissProtO13688Non-structural maintenance of chromosome element 6
ModBaseO13688Database of comparative protein structure models
STRINGO13688Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594933Smc5-6 complex non-SMC subunit Nse6
RefSeq mRNANM_001020364972h- Smc5-6 complex non-SMC subunit Nse6 (nse6), mRNA
European Nucleotide ArchiveAJ002494ENA EMBL mapping
European Nucleotide ArchiveCAB11187.1ENA Protein Mapping
UniParcUPI0000127F06UniProt Archive
ArrayExpressE-MTAB-2543deletion mutant expression profiling

Literature for nse6

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015