nse6 (SPAC11E3.08c)

Gene Standard Namense6 Characterisation Statuspublished
Systematic IDSPAC11E3.08c Feature Typeprotein coding
Synonyms Name Description
ProductSmc5-6 complex non-SMC subunit Nse6 Product Size522aa, 59.90 kDa
Genomic Location Chromosome I, 5297794-5296049 (1746nt); CDS:5297733-5296165 (1569nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding848
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular response to DNA damage stimulus214
Annotation ExtensionEvidenceWith/FromReference
DNA recombination116
Annotation ExtensionEvidenceWith/FromReference
replication fork protection6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Smc5-Smc6 complex8
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to 4-nitroquinoline N-oxide159
expressivity FYPO_EXT:0000002nse6ΔNull
sensitive to camptothecin214
expressivity FYPO_EXT:0000001nse6ΔNull
sensitive to hydroxyureanse6ΔNull518
expressivity FYPO_EXT:0000001nse6ΔNull
expressivity FYPO_EXT:0000002nse6ΔNull
sensitive to ionizing radiation58
expressivity FYPO_EXT:0000002nse6ΔNull
sensitive to methyl methanesulfonate227
expressivity FYPO_EXT:0000001nse6ΔNull
sensitive to UV during vegetative growthnse6ΔNull133
viable vegetative cell populationnot recorded (unrecorded)3781

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclense6ΔNull794
cut during cellular response to hydroxyurea43
penetrance FYPO_EXT:0000002nse6ΔNull
elongated vegetative cell703
penetrance FYPO_EXT:0000003not recorded (unrecorded)
viable elongated vegetative cell231
penetrance FYPO_EXT:0000001nse6ΔNull
penetrance FYPO_EXT:0000001, expressivity FYPO_EXT:0000001nse6ΔNull
Ensembl transcript structure with UTRs, exons and introns


Exon Start End
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08691 Pfam IPR014803 DNA repair protein Nse5/Nse6 1 474 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.75 Da
Charge 5.50
Isoelectric point 7.91
Molecular weight 59.90 kDa
Number of residues 522

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS29
present during mitotic M phaseS26
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S26 PMID:21712547
present during mitotic M phase experimental evidence S29 PMID:21712547
Gene Expression

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.46during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
0.14during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
Subunit Composition
homomeric(2) PMID:1647898448
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi only619
conserved in fungi4604
conserved in eukaryotes4516

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
nse2Smc5-6 complex non-SMC subunit 2, SUMO ligase Affinity Capture-WesternPMID:16478984
Affinity Capture-MS
smc6Smc5-6 complex SMC subunit Smc6 Affinity Capture-MSPMID:16478984
Affinity Capture-Western
Reconstituted Complex
Reconstituted ComplexPMID:17005570
nse1Smc5-6 complex ubiquitin-protein ligase E3 subunit 1 Affinity Capture-MSPMID:16478984
Affinity Capture-Western
smc5Smc5-6 complex SMC subunit Smc5 Affinity Capture-MSPMID:16478984
Reconstituted Complex
Reconstituted ComplexPMID:17005570
nse3Smc5-6 complex non-SMC subunit Nse3 Affinity Capture-MSPMID:16478984
nse4Smc5-6 complex non-SMC subunit Nse4 Affinity Capture-MSPMID:16478984
nse5Smc5-6 complex non-SMC subunit Nse5 Affinity Capture-WesternPMID:16478984
Affinity Capture-MS
Reconstituted Complex
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
pnk1DNA kinase/phosphatase Pnk1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
hip1hira protein, histone chaperone Hip1 Positive GeneticPMID:22681890
mph1dual specificity protein kinase Mph1 Negative GeneticPMID:22681890
klp6kinesin-like protein Klp6 Negative GeneticPMID:22681890
csn4COP9/signalosome complex subunit Csn4 Negative GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
SPCC16C4.106-phosphogluconolactonase (predicted) Positive GeneticPMID:22681890
dbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
ctf18RFC-like complex subunit Ctf18 Synthetic Growth DefectPMID:18931302
bag101BAG family molecular chaperone regulator Bag101 (predicted) Negative GeneticPMID:22681890
rad55RecA family ATPase Rad55/Rhp55 Synthetic Growth DefectPMID:16478984
Synthetic Rescue
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Positive GeneticPMID:21504829
pof8F-box protein Pof8 Synthetic Growth DefectPMID:18931302
mus81Holliday junction resolvase subunit Mus81 Synthetic LethalityPMID:22855558
Synthetic LethalityPMID:16478984
rhp7Rad7 homolog Rhp7 Negative GeneticPMID:22681890
nse2Smc5-6 complex non-SMC subunit 2, SUMO ligase Synthetic LethalityPMID:16478984
pzh1serine/threonine protein phosphatase Pzh1 Negative GeneticPMID:22681890
yam8stretch-activated calcium channel Yam8 Synthetic Growth DefectPMID:18931302
rqh1RecQ type DNA helicase Rqh1 Synthetic LethalityPMID:16478984
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
rad3ATR checkpoint kinase Rad3 Synthetic Growth DefectPMID:18931302
Synthetic LethalityPMID:16478984
Negative GeneticPMID:22681890
dis2serine/threonine protein phosphatase PP1 subfamily, Dis2 Synthetic Growth DefectPMID:21965289
csn1COP9/signalosome complex subunit Csn1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
usp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
ssp2serine/threonine protein kinase Ssp2 Positive GeneticPMID:22681890
fkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:22681890
rad51RecA family recombinase Rad51/Rhp51 Synthetic RescuePMID:22855558
Synthetic RescuePMID:16478984
Positive GeneticPMID:22681890
nap1nucleosome assembly protein Nap1 Negative GeneticPMID:22681890
SPBC4B4.04translation initiation factor eIF2A (predicted) Negative GeneticPMID:22681890
pom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Negative GeneticPMID:22681890
dam1DASH complex subunit Dam1 Negative GeneticPMID:22681890
ams2cell cycle regulated GATA-type transcription factor Ams2 Positive GeneticPMID:22681890
duo1DASH complex subunit Duo1 Negative GeneticPMID:22681890
nth1DNA endonuclease III Negative GeneticPMID:22681890
cwf19complexed with Cdc5 protein Cwf19 Negative GeneticPMID:22681890
tdp1tyrosyl-DNA phosphodiesterase Tdp1 Synthetic Growth DefectPMID:18931302
uve1endonuclease Uve1 Synthetic Growth DefectPMID:16478984
fbh1DNA helicase I, ubiquitin ligase F-box adaptor Fbh1 Synthetic Growth DefectPMID:18931302
csn3COP9/signalosome complex subunit Csn3 (predicted) Negative GeneticPMID:22681890
rxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
SPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:22681890
raf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
dbl8tRNA-splicing endonuclease positive effector Dbl8 Negative GeneticPMID:22681890
rad26ATRIP, ATR checkpoint kinase regulatory subunit Rad26 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
nse3Smc5-6 complex non-SMC subunit Nse3 Synthetic LethalityPMID:16478984
dad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
pli1SUMO E3 ligase Pli1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
swi5Swi5 protein Synthetic RescuePMID:16478984
mcs4response regulator Mcs4 Synthetic Growth DefectPMID:18931302
dcc1Ctf18 RFC-like complex subunit Dcc1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
clr3histone deacetylase (class II) Clr3 Negative GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
rad60DNA repair protein Rad60 Synthetic LethalityPMID:16478984
pub3HECT-type ubiquitin-protein ligase E3 Pub3 (predicted) Synthetic Growth DefectPMID:18931302
SPBC1861.07elongin C (predicted) Negative GeneticPMID:22681890
trt1telomerase reverse transcriptase 1 protein Trt1 Negative GeneticPMID:22681890
sfp1transcription factor Sfp1 (predicted) Positive GeneticPMID:22681890
SPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
mug80cyclin Clg1 (predicted) Positive GeneticPMID:22681890
csn71COP9/signalosome complex subunit 7a (predicted) Synthetic Growth DefectPMID:18931302
elp2elongator complex subunit Elp2 (predicted) Positive GeneticPMID:22681890
ppa2serine/threonine protein phosphatase Ppa2 Positive GeneticPMID:22681890
trm10tRNA m(1)G methyltransferase Trm10 Negative GeneticPMID:22681890
ase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
pht1histone H2A variant H2A.Z, Pht1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
rtt109RTT109 family histone lysine acetyltransferase Negative GeneticPMID:22681890
rec12meiotic recombination endonuclease Rec12 Synthetic RescuePMID:22855558
nup132nucleoporin Nup132 Negative GeneticPMID:22681890
srp1SR family protein, human SRFS2 ortholog Srp1 Negative GeneticPMID:22681890
nts1Schizosaccharomyces specific protein Dosage RescuePMID:25002536
SPAC12B10.15cribonuclease H2 complex subunit (predicted) Synthetic Growth DefectPMID:18931302
ste20Rictor homolog, Ste20 Positive GeneticPMID:22681890
prp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Positive GeneticPMID:22681890
spc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
rox3mediator complex subunit Med19/Rox3 Positive GeneticPMID:22681890
chk1Chk1 protein kinase Synthetic Growth DefectPMID:16478984
nto1histone acetyltransferase complex subunit Nto1 (predicted) Negative GeneticPMID:22681890
snt2Lid2 complex subunit Snt2 Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Positive GeneticPMID:22681890
cph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
srs2ATP-dependent DNA helicase, UvrD subfamily Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
rpb7DNA-directed RNA polymerase complex II subunit Rpb7 Positive GeneticPMID:22681890
csn2COP9/signalosome complex subunit Csn2 Negative GeneticPMID:22681890
mrc1mediator of replication checkpoint 1 Synthetic Growth DefectPMID:18931302
rad13DNA repair nuclease Rad13 Synthetic Growth DefectPMID:18931302
Synthetic Growth DefectPMID:16478984
cpd1tRNA (m1A) methyltransferase complex catalytic subunit Cpd1 Positive GeneticPMID:22681890
tip1CLIP170 family protein Tip1 Negative GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
rhp18Rad18 homolog ubiquitin protein ligase E3, Rhp18 Synthetic Growth DefectPMID:18931302
Synthetic Growth DefectPMID:16478984
pku80Ku domain protein Pku80 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
cgi121EKC/KEOPS complex subunit Cgi121 (predicted) Positive GeneticPMID:22681890
apn2AP-endonuclease Apn2 Negative GeneticPMID:22681890
dmc1RecA family ATPase Dmc1 Synthetic RescuePMID:22855558
Positive GeneticPMID:22681890
swi10DNA repair endonuclease Swi10 Synthetic Growth DefectPMID:18931302
hrq1RecQ type DNA helicase Hrq1 (predicted) Synthetic Growth DefectPMID:22064477
Negative GeneticPMID:22681890
nup40nucleoporin Nup40 Negative GeneticPMID:22681890
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
csn5COP9/signalosome complex subunit Csn5 Negative GeneticPMID:22681890
mug154conserved fungal protein Negative GeneticPMID:22681890
rsv2transcription factor Rsv2 Negative GeneticPMID:22681890
pob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
hif2Set3 complex subunit Hif2 Negative GeneticPMID:22681890
rhp14XP-A family homolog Rhp14 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
res2MBF transcription factor complex subunit Res2 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
amk2AMP-activated protein kinase beta subunit Amk2 Positive GeneticPMID:22681890
raf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Negative GeneticPMID:22681890
ssu72phosphoric ester hydrolase Ssu72 (predicted) Negative GeneticPMID:22681890
nsk1Clp1-interacting, microtubule plus-end binding Nsk1 Negative GeneticPMID:22681890
SPBC36B7.08cnucleosome assembly protein (predicted) Negative GeneticPMID:22681890
swi3replication fork protection complex subunit Swi3 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
ckb2CK2 family regulatory subunit Ckb2 (predicted) Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC11E3.08c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC11E3.08c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC11E3.08c BioGRID Interaction Datasets
Expression Viewer SPAC11E3.08c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC11E3.08c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC11E3.08c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC11E3.08c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC11E3.08c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC11E3.08c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC11E3.08c Cell Cycle Data
GEO SPAC11E3.08c GEO profiles
PInt SPAC11E3.08c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC11E3.08c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC11E3.08c Fission yeast phenotypic data & analysis
SPD / RIKEN52/52B04Orfeome Localization Data
UniProtKB/SwissProtO13688Non-structural maintenance of chromosome element 6
ModBaseO13688Database of comparative protein structure models
STRINGO13688Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594933Smc5-6 complex non-SMC subunit Nse6
RefSeq mRNANM_001020364972h- Smc5-6 complex non-SMC subunit Nse6 (nse6), mRNA
European Nucleotide ArchiveAJ002494ENA EMBL mapping
European Nucleotide ArchiveCAB11187.1ENA Protein Mapping
UniParcUPI0000127F06UniProt Archive

Literature for nse6

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015