ubi1 (SPAC11G7.04)

Gene Standard Nameubi1 Characterisation Statusbiological role inferred
Systematic IDSPAC11G7.04 Feature Typeprotein coding
Synonyms Name Description
Productribosomal-ubiquitin fusion protein Ubi1 (predicted) Product Size128aa, 14.60 kDa
Genomic Location Chromosome I, 3968932-3969558 (627nt); CDS:3969009-3969395 (387nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
structural constituent of ribosome256
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translation471
Annotation ExtensionEvidenceWith/FromReference
ribosome biogenesis339
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosolic large ribosomal subunit82
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
sensitive to 4-nitroquinoline N-oxideubi1Δ159
sensitive to bortezomibubi1Δ255
sensitive to camptothecinubi1Δ259
sensitive to hydroxyureaubi1Δ595
sensitive to ionizing radiation during vegetative growthubi1Δ76
sensitive to methyl methanesulfonateubi1Δ333
viable vegetative cell populationubi1Δ3862

Cell Phenotype

Term NameGenotypesCount
abnormal microtubule cytoskeleton morphology during mitotic interphaseubi1Δ187
vegetative cell lysisubi1Δ98
viable vegetative cell with abnormal cell shapeubi1Δ709
viable vegetative cell, abnormal cell shape, normal cell sizeubi1Δ215
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR3968932..3969008PMID:21511999
3' UTR3969396..3969558PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00240 Pfam IPR000626 Ubiquitin domain 3 74 14
PF01020 Pfam IPR001975 Ribosomal protein L40e 78 127 2
SM01377 SMART IPR001975 Ribosomal protein L40e 77 128 2
SM00213 SMART IPR000626 Ubiquitin domain 1 72 13
PS50053 Prosite Profiles IPR000626 Ubiquitin domain 1 76 18
PS00299 Prosite Patterns IPR019954 Ubiquitin conserved site 27 52 7
PTHR10666 HMMPANTHER 1 127 6 Gene3D Membrane-anchored ubiquitin-fold protein 1 96 28
SSF54236 SuperFamily IPR029071 Ubiquitin-related domain 1 95 29
PR00348 PRINTS IPR019956 Ubiquitin 32 52 6
PR00348 PRINTS IPR019956 Ubiquitin 11 31 6
PR00348 PRINTS IPR019956 Ubiquitin 53 74 6

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001447ubiquitin family proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001447
PBO:0001733ribosomal-ubiquitin fusion proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001733

Protein Properties

Ave. residue weight 114.02 Da
Charge 15.00
Codon Adaptation Index 0.74
Isoelectric point 10.48
Molecular weight 14.60 kDa
Number of residues 128

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2293
present during cellular response to thiabendazoleS57
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S57 PMID:18257517
IDA S57 PMID:25720772
O-phospho-L-threonineT55 1087
Annotation ExtensionEvidenceResidueReference
IDA T55 PMID:25720772
ubiquitinylated lysineK6, K11, K27, K29, K48, K63 514
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence K48 PMID:26412298
mass spectrometry evidence K27 PMID:26412298
mass spectrometry evidence K63 PMID:26412298
mass spectrometry evidence K6 PMID:26412298
IDA PMID:26412298
mass spectrometry evidence K29 PMID:26412298
mass spectrometry evidence K11 PMID:26412298
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for ubi1 (SPAC11G7.04)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3127.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
43during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
5.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in metazoa3498
conserved in vertebrates3473
conserved in eukaryotes only2525

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC11G7.04 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withrhp23Rad23 homolog Rhp23 Two-hybridPMID:11788722
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC11G7.04 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
overexpression rescuesecm33cell wall protein Ecm33 Dosage RescuePMID:24454826
overexpression rescuesapm1AP-1 adaptor complex mu subunit Apm1 Dosage RescuePMID:24454826
overexpression rescuescis4cation diffusion family zinc transmembrane transporter Cis4 Dosage RescuePMID:24454826
External References
Database Identifier Description
NBRP SPAC11G7.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC11G7.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC11G7.04 BioGRID Interaction Datasets
Expression Viewer SPAC11G7.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC11G7.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC11G7.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC11G7.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC11G7.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC11G7.04 Transcriptome Viewer (Bähler Lab)
GEO SPAC11G7.04 GEO profiles
PInt SPAC11G7.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC11G7.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC11G7.04 Fission yeast phenotypic data & analysis
Cyclebase SPAC11G7.04.1 Cell Cycle Data
UniProtKB/SwissProtP0CH06Ubiquitin-60S ribosomal protein L40 Ubiquitin 60S ribosomal protein L40
ModBaseP0CH06Database of comparative protein structure models
STRINGP0CH06Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594398ubiquitin-60S ribosomal protein L40 fusion protein
RefSeq mRNANM_001019821972h- ubiquitin-60S ribosomal protein L40 fusion protein (ubi1), mRNA
European Nucleotide ArchiveCAB16209.1ENA Protein Mapping
UniParcUPI0000069E71UniProt Archive

Literature for ubi1

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017