cid14 (SPAC12G12.13c)


Gene Standard Namecid14 Characterisation Statuspublished
Systematic IDSPAC12G12.13c Feature Typeprotein coding
Synonyms Name Description
Productpoly(A) polymerase Cid14 Product Size684aa, 78.30 kDa
Genomic Location Chromosome I, 321764-324129 (2366nt); CDS:321924-323978 (2055nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
metal ion binding750
Annotation ExtensionEvidenceWith/FromReference
polynucleotide adenylyltransferase activity7
Annotation ExtensionEvidenceWith/FromReference
ribosomal large subunit binding3
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ncRNA polyadenylation involved in polyadenylation-dependent ncRNA catabolic process4
Annotation ExtensionEvidenceWith/FromReference
nuclear polyadenylation-dependent rRNA catabolic process14
Annotation ExtensionEvidenceWith/FromReference
RNA processing474
Annotation ExtensionEvidenceWith/FromReference
snoRNA catabolic process2
Annotation ExtensionEvidenceWith/FromReference
tRNA catabolic process16
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nucleolus366
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
TRAMP complex4
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at low temperaturecid14Δ85
decreased cell population growth on glucose carbon sourcecid14Δ293
decreased vegetative cell population growthcid14Δ634
inviable vegetative cell populationcid14+ (wild type)1450
sensitive to 5-fluorouracilcid14Δ273
slow vegetative cell population growthcid14Δ340
viable vegetative cell populationcid14Δ3831

Cell Phenotype

Term NameGenotypesCount
abnormal chromatin silencing at silent mating-type cassettecid14Δ28
abnormal mitotic cell cyclecid14Δ849
abnormal nucleolar morphologycid14Δ4
complete but unequal mitotic sister chromatid segregationcid14Δ34
decreased chromatin silencingcid14Δ68
decreased polyadenylated 5.8S rRNA levelcid14Δ1
increased minichromosome loss during vegetative growthcid14Δ64
increased RNA level during vegetative growth222
affecting mei2cid14Δ
affecting SO:0001923cid14Δ
affecting SO:0001925cid14Δ
increased snoRNA level3
affecting sno12cid14Δ
normal chromatin silencing at centromerecid14Δ11
normal histone H3-K9 dimethylation at silent mating-type cassettecid14Δ3
normal histone H3-K9 dimethylation at telomerecid14Δ2
normal level of DSR-containing meiosis gene mRNA during vegetative growthcid14Δ3
normal mitotic sister chromatid cohesioncid14Δ2
normal polyadenylated 5S rRNA levelcid14Δ1
normal RNA level during vegetative growth120
affecting SO:0001924cid14Δ
affecting SO:0001926cid14Δ
polyadenylated 25S rRNA absent from cellDADA (D298A, D230A)1
cid14Δ
unequal nucleolus inheritancecid14Δ1
viable elongated vegetative cellcid14Δ313
viable vegetative cell with normal cell morphologycid14Δ3099

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
inviable vegetative cell populationcid14Δ, bub1Δ
cid14Δ, pab2Δ
viable vegetative cell populationcid14Δ, top1Δ

Cell Phenotype

Term NameGenotypes
abnormal snRNA 3'-end processing
affecting SO:0000396cid14Δ, mpn1Δ
decreased histone H3-K9 dimethylation at silent mating-type cassetteswi6Δ, cid14Δ
increased level of heterochromatin-encoded proteinsswi6Δ, cid14Δ
cid14Δ, clr4Δ
increased snoRNA level
affecting sno12cid14Δ, pab2Δ
normal histone H3-K9 dimethylation at telomereswi6Δ, cid14Δ
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons321764..324129
5' UTR321764..321923PMID:21511999
3' UTR323979..324129PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01909 Pfam IPR002934 Polymerase, nucleotidyl transferase domain 266 378 6
PF03828 Pfam IPR002058 PAP/25A-associated 434 492 5
PTHR23092 HMMPANTHER 133 683 2
PTHR23092:SF15 HMMPANTHER 133 683 1
3.30.460.10 Gene3D CCA-adding enzyme, firmicutes 254 403 11
SSF81301 SuperFamily 224 391 11
SSF81631 SuperFamily 379 520 7

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.47 Da
Charge 8.50
Codon Adaptation Index 0.41
Isoelectric point 7.76
Molecular weight 78.30 kDa
Number of residues 684
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1675
present during mitotic M phaseS658
S163, S169
present during cellular response to thiabendazole, mitotic M phaseS163
present during mitotic M phaseS602
present during mitotic M phaseS600
Annotation ExtensionEvidenceResidueReference
experimental evidence S163 PMID:24763107
present during mitotic M phase experimental evidence S163 PMID:21712547
present during cellular response to thiabendazole IDA S163 PMID:18257517
experimental evidence S169 PMID:24763107
present during mitotic M phase experimental evidence S600 PMID:21712547
present during mitotic M phase experimental evidence S602 PMID:21712547
present during mitotic M phase experimental evidence S658 PMID:21712547
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelconstant during GO:0000278Western blot evidencePMID:16478992

Quantitative Gene Expression

View graphical display of gene expression data for cid14 (SPAC12G12.13c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2954during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3018during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3174during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2530during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
703.79during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2732during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
398.03during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.6during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complements S. cerevisiae TRF4 PMID:16478992
Misc
DescriptionQualifierReferenceCount
deletion mutant expression profiling PMID:1802510577
heterochromatic mRNAs transcribed in cid14delta are not translated PMID:226832691
Warning
DescriptionQualifierReferenceCount
gene structure updated433
Pathway
DescriptionQualifierReferenceCount
Mad2-dependent checkpoint response PMID:164789927
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3422
conserved in vertebrates3397
conserved in eukaryotes only2504
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPAC12G12.13c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycrp79poly(A) binding protein Crp79 Affinity Capture-RNAPMID:20531409
Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPAC12G12.13c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
negative genetic interaction withhip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
negative genetic interaction withace2transcription factor Ace2 Negative GeneticPMID:22681890
negative genetic interaction withpyp1tyrosine phosphatase Pyp1 Negative GeneticPMID:22681890
negative genetic interaction withusp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
negative genetic interaction withmrc1mediator of replication checkpoint 1 Negative GeneticPMID:22681890
negative genetic interaction withvps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
negative genetic interaction withSPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction witheso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
negative genetic interaction withdbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
negative genetic interaction withtea2kinesin-like protein Tea2 Negative GeneticPMID:22681890
negative genetic interaction withpar1protein phosphatase PP2A regulatory subunit B-56 Par1 Negative GeneticPMID:22681890
negative genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
negative genetic interaction withsep1forkhead transcription factor Sep1 Negative GeneticPMID:22681890
negative genetic interaction withtea1cell end marker Tea1 Negative GeneticPMID:22681890
negative genetic interaction withtor1phosphatidylinositol kinase Tor1 Negative GeneticPMID:22681890
negative genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
negative genetic interaction withsty1MAP kinase Sty1 Negative GeneticPMID:22681890
negative genetic interaction withelp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withres2MBF transcription factor complex subunit Res2 Positive GeneticPMID:22681890
positive genetic interaction withamk2AMP-activated protein kinase beta subunit Amk2 Positive GeneticPMID:22681890
positive genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Positive GeneticPMID:22681890
positive genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Positive GeneticPMID:21504829
positive genetic interaction withdad2DASH complex subunit Dad2 Positive GeneticPMID:22681890
positive genetic interaction withrik1silencing protein Rik1 Positive GeneticPMID:22681890
positive genetic interaction withdad1DASH complex subunit Dad1 Positive GeneticPMID:22681890
positive genetic interaction withSPAC144.05ATP-dependent DNA helicase/ ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsts5RNB-like protein Positive GeneticPMID:22681890
positive genetic interaction withnup132nucleoporin Nup132 Positive GeneticPMID:22681890
positive genetic interaction withcaf4CCR4-Not complex subunit Caf4/Mdv1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPBPB10D8.02carylsulfatase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrad57RecA family ATPase Rad57/Rhp57 Positive GeneticPMID:22681890
rescuesrho2Rho family GTPase Rho2 Phenotypic SuppressionPMID:24820419
phenotype enhanced byerf4palmitoyltransferase complex subunit Erf4 Phenotypic EnhancementPMID:23843742
rescued byrho3Rho family GTPase Rho3 Phenotypic SuppressionPMID:23843742
External References
Database Identifier Description
NBRP SPAC12G12.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC12G12.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC12G12.13c BioGRID Interaction Datasets
Expression Viewer SPAC12G12.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC12G12.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC12G12.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC12G12.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC12G12.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC12G12.13c Transcriptome Viewer (Bähler Lab)
GEO SPAC12G12.13c GEO profiles
PInt SPAC12G12.13c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC12G12.13c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC12G12.13c Fission yeast phenotypic data & analysis
Cyclebase SPAC12G12.13c.1 Cell Cycle Data
IntEnz2.7.7.1Integrated relational Enzyme database
Rhea2.7.7.1Annotated reactions database
SPD / RIKEN26/26B12Orfeome Localization Data
UniProtKB/SwissProtQ9UTN3Poly(A) RNA polymerase cid14
ModBaseQ9UTN3Database of comparative protein structure models
STRINGQ9UTN3Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018181poly(A) polymerase Cid14
RefSeq mRNANM_001018285972h- poly(A) polymerase Cid14 (cid14), mRNA
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveCAI79317ENA Protein Mapping
UniParcUPI00005053CCUniProt Archive

Literature for cid14

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016