fur4 (SPAC1399.03)


Gene Standard Namefur4 Characterisation Statuspublished
Systematic IDSPAC1399.03 Feature Typeprotein coding
Synonyms Name Description
Producturacil permease Product Size581aa, 64.18 kDa
Genomic Location Chromosome I, 1843995-1841908 (2088nt); CDS:1843681-1841936 (1746nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0015505uracil:cation symporter activityISOSGD:S000000225PMID:97302842
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006812cation transportISOSGD:S000000225PMID:9730284136
GO:0034220ion transmembrane transportISOSGD:S000000225PMID:9730284189
GO:0015931nucleobase-containing compound transportISOSGD:S000000225PMID:973028484
GO:0072531pyrimidine-containing compound transmembrane transportISOSGD:S000000225PMID:97302849
GO:1902431uracil import into cellIGIura4PMID:67651741
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005794Golgi apparatusIDAPMID:16823372355
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371017
GO:0045121membrane raftISOSGD:S000000225GO_REF:00000242
GO:0005886plasma membraneISOSGD:S000000225GO_REF:0000024242
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001986resistance to 5-fluorouracilCell growth assayfur4ΔNullPMID:97302841
FYPO:0002060viable vegetative cell populationMicroscopyfur4ΔNullPMID:204732893760
Microscopyfur4ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3086
penetrance FYPO_EXT:0000001Microscopyfur4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
118439951841908

UTRs

Region Coordinates Reference
five_prime_UTR1843995..1843682PMID:18641648
three_prime_UTR1841935..1841908PMID:18641648
five_prime_UTR1843995..1843682,1843995..1843682PMID:20118936
three_prime_UTR1841935..1841908,1841935..1841908PMID:20118936
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02133 Pfam IPR001248 Permease, cytosine/purines, uracil, thiamine, allantoin 48 499 3
TMhelix TMHMM 210 232 959
TMhelix TMHMM 187 206 959
TMhelix TMHMM 287 309 959
TMhelix TMHMM 494 516 959
TMhelix TMHMM 459 481 959
TMhelix TMHMM 253 272 959
TMhelix TMHMM 410 432 959
TMhelix TMHMM 384 403 959
TMhelix TMHMM 88 110 959
TMhelix TMHMM 342 364 959
PTHR30618 HMMPANTHER 2 576 3
PTHR30618:SF2 HMMPANTHER 2 576 2
TIGR00800 tigrfam IPR012681 Nucleobase cation symporter-1, NCS1 43 484 2

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 110.46 Da
Charge 7.00
Isoelectric point 7.65
Molecular weight 64.18 kDa
Number of residues 581
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS573PMID:217125471663
present during mitotic M phase
present during cellular response to thiabendazoleIDAS570PMID:18257517
S570PMID:24763107
MOD:00696phosphorylated residueNASPMID:182575171915
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
21611.39during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
2435.84during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
4.3during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
3.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae FUR4full PMID:6765174
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPAC1399.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1399.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1399.03 BioGRID Interaction Datasets
Expression Viewer SPAC1399.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1399.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1399.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1399.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1399.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1399.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1399.03 Cell Cycle Data
GEO SPAC1399.03 GEO profiles
PInt SPAC1399.03 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542586uracil permease
EntrezGene2542586uracil permease
SPD / RIKEN49/49B11Orfeome Localization Data
UniProtKB/SwissProtQ10279Uracil permease
ModBaseQ10279Database of comparative protein structure models
STRINGQ10279Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593511uracil permease
RefSeq mRNANM_001018945972h- uracil permease (fur4), mRNA
European Nucleotide ArchiveCAA67256ENA Protein Mapping
European Nucleotide ArchiveCAA67256.1ENA Protein Mapping
European Nucleotide ArchiveCAC19744ENA Protein Mapping
European Nucleotide ArchiveCAC19744.1ENA Protein Mapping
UniParcUPI000012AD72UniProt Archive

Literature for fur4

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014