fur4 (SPAC1399.03)


Gene Standard Namefur4 Characterisation Statuspublished
Systematic IDSPAC1399.03 Feature Typeprotein coding
Synonyms Name Description
Producturacil permease Product Size581aa, 64.18 kDa
Genomic Location Chromosome I, 1843995-1841908 (2088nt); CDS:1843681-1841936 (1746nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
uracil:cation symporter activity2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cation transmembrane transport128
Annotation ExtensionEvidenceWith/FromReference
nucleobase-containing compound transport80
Annotation ExtensionEvidenceWith/FromReference
pyrimidine-containing compound transmembrane transport10
Annotation ExtensionEvidenceWith/FromReference
uracil import across plasma membrane1
Annotation ExtensionEvidenceWith/FromReference
uracil transport2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Golgi apparatus355
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
plasma membrane raft2
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001986resistance to 5-fluorouracilCell growth assayfur4ΔNullPMID:97302841
FYPO:0002060viable vegetative cell populationMicroscopyfur4ΔNullPMID:204732893759
Microscopyfur4ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyfur4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
118439951841908

UTRs

Region Coordinates Reference
five_prime_UTR1843995..1843682PMID:18641648
three_prime_UTR1841935..1841908PMID:18641648
five_prime_UTR1843995..1843682PMID:20118936
three_prime_UTR1841935..1841908PMID:20118936
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02133 Pfam IPR001248 Permease, cytosine/purines, uracil, thiamine, allantoin 48 499 3
TMhelix TMHMM 210 232 959
TMhelix TMHMM 187 206 959
TMhelix TMHMM 287 309 959
TMhelix TMHMM 494 516 959
TMhelix TMHMM 459 481 959
TMhelix TMHMM 253 272 959
TMhelix TMHMM 384 403 959
TMhelix TMHMM 410 432 959
TMhelix TMHMM 88 110 959
TMhelix TMHMM 342 364 959
PTHR30618:SF0 HMMPANTHER 12 576 3
PTHR30618 HMMPANTHER 12 576 3
TIGR00800 tigrfam IPR012681 Nucleobase cation symporter-1, NCS1 43 484 2

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 110.46 Da
Charge 7.00
Isoelectric point 7.65
Molecular weight 64.18 kDa
Number of residues 581
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS573PMID:217125471670
present during mitotic M phase
experimental evidenceS570PMID:24763107
present during cellular response to thiabendazoleIDAS570PMID:18257517
MOD:00696phosphorylated residueNASPMID:182575171922
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2435.84during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
21611.39during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.3during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae FUR4full PMID:6765174
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPAC1399.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1399.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1399.03 BioGRID Interaction Datasets
Expression Viewer SPAC1399.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1399.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1399.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1399.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1399.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1399.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1399.03 Cell Cycle Data
GEO SPAC1399.03 GEO profiles
PInt SPAC1399.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1399.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1399.03 Fission yeast phenotypic data & analysis
SPD / RIKEN49/49B11Orfeome Localization Data
UniProtKB/SwissProtQ10279Uracil permease
ModBaseQ10279Database of comparative protein structure models
STRINGQ10279Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593511uracil permease
RefSeq mRNANM_001018945972h- uracil permease (fur4), mRNA
European Nucleotide ArchiveCAA67256.1ENA Protein Mapping
European Nucleotide ArchiveCAC19744.1ENA Protein Mapping
UniParcUPI000012AD72UniProt Archive

Literature for fur4

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014