fur4 (SPAC1399.03)

Gene Standard Namefur4 Characterisation Statuspublished
Systematic IDSPAC1399.03 Feature Typeprotein coding
Synonyms Name Description
Producturacil permease Product Size581aa, 64.18 kDa
Genomic Location Chromosome I, 1843995-1841908 (2088nt); CDS:1843681-1841936 (1746nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
uracil transmembrane transporter activity2
Annotation ExtensionEvidenceWith/FromReference
uracil:cation symporter activity2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
uracil import across plasma membrane2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell surface89
Annotation ExtensionEvidenceWith/FromReference
endoplasmic reticulum602
Annotation ExtensionEvidenceWith/FromReference
fungal-type vacuole131
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus358
Annotation ExtensionEvidenceWith/FromReference
Golgi membrane56
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane969
Annotation ExtensionEvidenceWith/FromReference
plasma membrane raft3
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
resistance to 5-fluorouracilfur4Δ13
viable vegetative cell populationfur4Δ3862

Cell Phenotype

Term NameGenotypesCount
abolished uracil importfur4Δ1
viable vegetative cell with normal cell morphologyfur4Δ3103

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
resistance to 5-fluorouracilfur4Δ, pub1Δ
sensitive to 5-fluorouracilfur4Δ, ura4Δ, pub1Δ

Cell Phenotype

Term NameGenotypes
decreased uracil importfur4Δ, ura4Δ, pub1Δ
Target Of
GO regulated by pub1 HECT-type ubiquitin-protein ligase E3 Pub1
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR1843995..1843682PMID:18641648
5' UTR1843995..1843682PMID:20118936
3' UTR1841935..1841908PMID:18641648
3' UTR1841935..1841908PMID:20118936
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02133 Pfam IPR001248 Purine-cytosine permease 48 499 3
TMhelix TMHMM 342 364 950
TMhelix TMHMM 187 206 950
TMhelix TMHMM 384 403 950
TMhelix TMHMM 459 481 950
TMhelix TMHMM 253 272 950
TMhelix TMHMM 210 232 950
TMhelix TMHMM 410 432 950
TMhelix TMHMM 287 309 950
TMhelix TMHMM 88 110 950
TMhelix TMHMM 494 516 950
PTHR30618 HMMPANTHER 1 579 3
PTHR30618:SF2 HMMPANTHER 1 579 2
TIGR00800 TIGRFAM IPR012681 Nucleobase cation symporter-1, NCS1 43 484 2

View domain organization at Pfam

Term IDTerm NameReferenceCount

Protein Properties

Ave. residue weight 110.46 Da
Charge 7.00
Codon Adaptation Index 0.56
Isoelectric point 7.65
Molecular weight 64.18 kDa
Number of residues 581

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2293
present during mitotic M phaseS573
present during cellular response to thiabendazoleS570
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S573 PMID:21712547
present during cellular response to thiabendazole IDA S570 PMID:18257517
IDA S570 PMID:25720772
experimental evidence S570 PMID:24763107
phosphorylated residue 2515
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysine 514
Annotation ExtensionEvidenceResidueReference
IDA PMID:26536126
O-phosphorylated residueS570,S573, S570,T572 2461
Annotation ExtensionEvidenceResidueReference
IDA S570,S573 PMID:25720772
IDA S570,T572 PMID:25720772
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for fur4 (SPAC1399.03)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2435.84during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
21611.39during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.3during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
3.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
functionally complemented by S. cerevisiae FUR4full PMID:6765174
Taxonomic Conservation
conserved in fungi4608
conserved in eukaryotes4516
conserved in bacteria1005

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC1399.03 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withura4orotidine 5'-phosphate decarboxylase Ura4 Synthetic Growth DefectPMID:26536126
External References
Database Identifier Description
NBRP SPAC1399.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1399.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1399.03 BioGRID Interaction Datasets
Expression Viewer SPAC1399.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1399.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1399.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1399.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1399.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1399.03 Transcriptome Viewer (Bähler Lab)
GEO SPAC1399.03 GEO profiles
PInt SPAC1399.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1399.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1399.03 Fission yeast phenotypic data & analysis
Cyclebase SPAC1399.03.1 Cell Cycle Data
SPD / RIKEN49/49B11Orfeome Localization Data
UniProtKB/SwissProtQ10279Uracil permease
ModBaseQ10279Database of comparative protein structure models
STRINGQ10279Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593511uracil permease
RefSeq mRNANM_001018945972h- uracil permease (fur4), mRNA
European Nucleotide ArchiveCAA67256.1ENA Protein Mapping
European Nucleotide ArchiveCAC19744.1ENA Protein Mapping
UniParcUPI000012AD72UniProt Archive

Literature for fur4

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017