pre9 (SPAC13C5.01c)


Gene Standard Namepre9 Characterisation Statusbiological_role_inferred
Systematic IDSPAC13C5.01c Feature Typeprotein coding
SynonymsSPAC31A2.17c Name Description
Product20S proteasome complex subunit alpha 3 Pre9 (predicted) Product Size248aa, 27.93 kDa
Genomic Location Chromosome I, 425256-423926 (1331nt); CDS:425223-424477 (747nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
endopeptidase activity65
Annotation ExtensionEvidenceWith/FromReference
threonine-type endopeptidase activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
proteasome core complex, alpha-subunit complex7
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationpre9ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
viable spheroid vegetative cellpre9ΔNull50
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons425256..423926
mRNA425256..423926
5' UTR425256..425224PMID:21511999
CDS425223..424477
3' UTR424476..423926PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF10584 Pfam IPR000426 Proteasome alpha-subunit, N-terminal domain 5 27 7
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 28 215 14
SM00948 SMART IPR000426 Proteasome alpha-subunit, N-terminal domain 5 27 7
PS00854 Prosite Patterns IPR016050 Proteasome beta-type subunit, conserved site 35 82 8
PS00388 Prosite Patterns IPR000426 Proteasome alpha-subunit, N-terminal domain 5 27 7
PS51475 Prosite Profiles IPR023332 Proteasome A-type subunit 20 238 7
PTHR11599:SF13 HMMPANTHER 1 248 1
PTHR11599 HMMPANTHER 1 248 14
3.60.20.10 Gene3D IPR029055 Nucleophile aminohydrolases, N-terminal 4 243 19
SSF56235 SuperFamily IPR029055 Nucleophile aminohydrolases, N-terminal 4 244 22

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.60 Da
Charge -1.00
Isoelectric point 6.14
Molecular weight 27.93 kDa
Number of residues 248
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during cellular response to thiabendazoleS13
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S13 PMID:18257517
phosphorylated residue 1927
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
82605during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
79245during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
80073during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
85592during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
16565.87during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
81467during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
11931.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7.3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in bacteria1001
conserved in metazoa3424
conserved in vertebrates3399
conserved in archaea238
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypre620S proteasome complex subunit alpha 4 Pre6 Affinity Capture-MSPMID:20133687
affinity captured bypre820S proteasome complex subunit alpha 2, Pre8 (predicted) Affinity Capture-MSPMID:22307589
affinity captured byrpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20838651
affinity captured byuch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20838651
External References
Database Identifier Description
NBRP SPAC13C5.01c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13C5.01c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13C5.01c BioGRID Interaction Datasets
Expression Viewer SPAC13C5.01c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13C5.01c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13C5.01c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13C5.01c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13C5.01c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13C5.01c Transcriptome Viewer (Bähler Lab)
GEO SPAC13C5.01c GEO profiles
PInt SPAC13C5.01c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13C5.01c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13C5.01c Fission yeast phenotypic data & analysis
Cyclebase SPAC13C5.01c.1 Cell Cycle Data
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
SPD / RIKEN09/09A11Orfeome Localization Data
UniProtKB/SwissProtQ09682Probable proteasome subunit alpha type-3
ModBaseQ09682Database of comparative protein structure models
STRINGQ09682Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001712988972h- 20S proteasome component alpha 3 (predicted) (SPAC13C5.01c), mRNA
RefSeq PeptideXP_00171304020S proteasome component alpha 3 (predicted)
European Nucleotide ArchiveCAA90475.1ENA Protein Mapping
UniParcUPI0000132456UniProt Archive

Literature for pre9

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015