SPAC13C5.01c


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC13C5.01c Feature Typeprotein coding
SynonymsSPAC31A2.17c Name Description
Product20S proteasome complex subunit alpha 3 (predicted) Product Size248aa, 27.93 kDa
Genomic Location Chromosome I, 425256-423926 (1331nt); CDS:425223-424477 (747nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004175endopeptidase activity66
contributes_toISSUniProtKB:P23638GO_REF:0000001
GO:0004298threonine-type endopeptidase activityIEAUniProtKB-KW:KW-0888GO_REF:000003715
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0043161proteasome-mediated ubiquitin-dependent protein catabolic processISSUniProtKB:P23638GO_REF:0000001112
GO:0007346regulation of mitotic cell cycleNASGO_REF:0000051280
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722319
GO:0005634nucleusIDAPMID:168233722737
GO:0019773proteasome core complex, alpha-subunit complexISSUniProtKB:P23638GO_REF:00000017
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopySPAC13C5.01cΔNullPMID:204732893751
MicroscopySPAC13C5.01cΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002380viable spheroid vegetative cell40
penetrance FYPO_EXT:0000001MicroscopySPAC13C5.01cΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1425256423926
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 28 215 14
PF10584 Pfam IPR000426 Proteasome alpha-subunit, N-terminal domain 5 27 7
SM00948 SMART IPR000426 Proteasome alpha-subunit, N-terminal domain 5 27 7
PS00388 Prosite Patterns IPR000426 Proteasome alpha-subunit, N-terminal domain 5 27 7
PS00854 Prosite Patterns IPR016050 Proteasome beta-type subunit, conserved site 35 82 8
PS51475 Prosite Profiles IPR023332 Proteasome A-type subunit 20 238 7
PTHR11599:SF13 HMMPANTHER 1 247 1
PTHR11599 HMMPANTHER 1 247 14
3.60.20.10 Gene3D 4 243 19
SSF56235 SuperFamily Nucleophile aminohydrolases, N-terminal 4 244 22

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.60 Da
Charge -1.00
Isoelectric point 6.14
Molecular weight 27.93 kDa
Number of residues 248
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS13PMID:182575171670
present during cellular response to thiabendazole
MOD:00696phosphorylated residueNASPMID:182575171922
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
82605during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
79245during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
80073during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
85592during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
81467during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
16565.87during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
11931.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7.3during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in bacteria1002
conserved in metazoa3428
conserved in vertebrates3402
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
bag102BAG family molecular chaperone regulator Bag102 Affinity Capture-WesternPMID:24497846
Reconstituted Complex
uch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20838651
bag101BAG family molecular chaperone regulator Bag101 (predicted) Affinity Capture-WesternPMID:24497846
Reconstituted Complex
pre820S proteasome complex subunit alpha 2, Pre8 (predicted) Affinity Capture-MSPMID:22307589
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
rpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20838651
External References
Database Identifier Description
NBRP SPAC13C5.01c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13C5.01c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13C5.01c BioGRID Interaction Datasets
Expression Viewer SPAC13C5.01c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13C5.01c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13C5.01c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13C5.01c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13C5.01c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13C5.01c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC13C5.01c Cell Cycle Data
GEO SPAC13C5.01c GEO profiles
PInt SPAC13C5.01c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13C5.01c Peptides identified in tandem mass spectrometry proteomics experiments
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
SPD / RIKEN09/09A11Orfeome Localization Data
UniProtKB/SwissProtQ09682Probable proteasome subunit alpha type-3
ModBaseQ09682Database of comparative protein structure models
STRINGQ09682Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001712988972h- 20S proteasome component alpha 3 (predicted) (SPAC13C5.01c), mRNA
RefSeq PeptideXP_00171304020S proteasome component alpha 3 (predicted)
European Nucleotide ArchiveCAA90475.1ENA Protein Mapping
UniParcUPI0000132456UniProt Archive

Literature for SPAC13C5.01c

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014