pre9 (SPAC13C5.01c)

Gene Standard Namepre9 Characterisation Statusbiological_role_inferred
Systematic IDSPAC13C5.01c Feature Typeprotein coding
SynonymsSPAC31A2.17c Name Description
Product20S proteasome complex subunit alpha 3 Pre9 (predicted) Product Size248aa, 27.93 kDa
Genomic Location Chromosome I, 425256-423926 (1331nt); CDS:425223-424477 (747nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
endopeptidase activity65
Annotation ExtensionEvidenceWith/FromReference
threonine-type endopeptidase activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
proteasome-mediated ubiquitin-dependent protein catabolic process137
Annotation ExtensionEvidenceWith/FromReference
regulation of mitotic cell cycle258
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
proteasome core complex, alpha-subunit complex7
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopypre9ΔNullPECO:0000005, PECO:0000137PMID:236978063759

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002380viable spheroid vegetative cell42
penetrance FYPO_EXT:0000001Microscopypre9ΔNullPECO:0000005, PECO:0000137PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 28 215 14
PF10584 Pfam IPR000426 Proteasome alpha-subunit, N-terminal domain 5 27 7
SM00948 SMART IPR000426 Proteasome alpha-subunit, N-terminal domain 5 27 7
PS00854 Prosite Patterns IPR016050 Proteasome beta-type subunit, conserved site 35 82 8
PS00388 Prosite Patterns IPR000426 Proteasome alpha-subunit, N-terminal domain 5 27 7
PS51475 Prosite Profiles IPR023332 Proteasome A-type subunit 20 238 7
PTHR11599:SF13 HMMPANTHER 1 247 1
PTHR11599 HMMPANTHER 1 247 14 Gene3D 4 243 19
SSF56235 SuperFamily Nucleophile aminohydrolases, N-terminal 4 244 22

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.60 Da
Charge -1.00
Isoelectric point 6.14
Molecular weight 27.93 kDa
Number of residues 248

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
present during cellular response to thiabendazole
MOD:00696phosphorylated residueNASPMID:182575171922
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
82605during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
79245during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
80073during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
85592during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
16565.87during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
81467during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
11931.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7.3during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400
conserved in archaea237

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
bag102BAG family molecular chaperone regulator Bag102 Reconstituted ComplexPMID:24497846
Affinity Capture-Western
uch2ubiquitin C-terminal hydrolase Uch2 Affinity Capture-MSPMID:20838651
bag101BAG family molecular chaperone regulator Bag101 (predicted) Affinity Capture-WesternPMID:24497846
Reconstituted Complex
pre620S proteasome complex subunit alpha 4 Pre6 Affinity Capture-MSPMID:20133687
pre820S proteasome complex subunit alpha 2, Pre8 (predicted) Affinity Capture-MSPMID:22307589
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
rpn1119S proteasome regulatory subunit Rpn11 Affinity Capture-MSPMID:20838651
External References
Database Identifier Description
NBRP SPAC13C5.01c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13C5.01c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13C5.01c BioGRID Interaction Datasets
Expression Viewer SPAC13C5.01c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13C5.01c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13C5.01c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13C5.01c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13C5.01c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13C5.01c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC13C5.01c Cell Cycle Data
GEO SPAC13C5.01c GEO profiles
PInt SPAC13C5.01c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13C5.01c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13C5.01c Fission yeast phenotypic data & analysis
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
SPD / RIKEN09/09A11Orfeome Localization Data
UniProtKB/SwissProtQ09682Probable proteasome subunit alpha type-3
ModBaseQ09682Database of comparative protein structure models
STRINGQ09682Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001712988972h- 20S proteasome component alpha 3 (predicted) (SPAC13C5.01c), mRNA
RefSeq PeptideXP_00171304020S proteasome component alpha 3 (predicted)
European Nucleotide ArchiveCAA90475.1ENA Protein Mapping
UniParcUPI0000132456UniProt Archive

Literature for pre9

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014