pre9 (SPAC13C5.01c)


Gene Standard Namepre9 Characterisation Statusbiological role inferred
Systematic IDSPAC13C5.01c Feature Typeprotein coding
Synonyms Name Description
Product20S proteasome complex subunit alpha 3 Pre9 (predicted) Product Size248aa, 27.93 kDa
Genomic Location Chromosome I, 425256-423926 (1331nt); CDS:425223-424477 (747nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
endopeptidase activity65
Annotation ExtensionEvidenceWith/FromReference
threonine-type endopeptidase activity15
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
proteasome core complex, alpha-subunit complex7
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationpre9Δ3831
pre9Δ

Cell Phenotype

Term NameGenotypesCount
viable spheroid vegetative cellpre9Δ51
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons425256..423926
5' UTR425256..425224PMID:21511999
3' UTR424476..423926PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00227 Pfam IPR001353 Proteasome, subunit alpha/beta 28 215 14
PF10584 Pfam IPR000426 Proteasome alpha-subunit, N-terminal domain 5 27 7
SM00948 SMART IPR000426 Proteasome alpha-subunit, N-terminal domain 5 27 7
PS51475 Prosite Profiles IPR023332 Proteasome A-type subunit 20 238 7
PS00854 Prosite Patterns IPR016050 Proteasome beta-type subunit, conserved site 35 82 8
PS00388 Prosite Patterns IPR000426 Proteasome alpha-subunit, N-terminal domain 5 27 7
PTHR11599 HMMPANTHER 1 248 14
PTHR11599:SF13 HMMPANTHER 1 248 1
3.60.20.10 Gene3D IPR029055 Nucleophile aminohydrolases, N-terminal 4 243 19
SSF56235 SuperFamily IPR029055 Nucleophile aminohydrolases, N-terminal 4 244 22

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.60 Da
Charge -1.00
Codon Adaptation Index 0.39
Isoelectric point 6.14
Molecular weight 27.93 kDa
Number of residues 248
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1675
present during cellular response to thiabendazoleS13
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S13 PMID:18257517
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pre9 (SPAC13C5.01c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
82605during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
79245during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
80073during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
85592during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
81467during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
16565.87during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
11931.82during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7.3during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3422
conserved in vertebrates3397
conserved in archaea238
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPAC13C5.01c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withcis4cation diffusion family zinc transmembrane transporter Cis4 Two-hybridPMID:23695164
forms complex withcis4cation diffusion family zinc transmembrane transporter Cis4 Reconstituted ComplexPMID:18199682
Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPAC13C5.01c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withatg1801WD repeat protein involved in autophagy Atg18a Positive GeneticPMID:22681890
positive genetic interaction withnot3CCR4-Not complex subunit Not3/5 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsck2serine/threonine protein kinase Sck2 Positive GeneticPMID:24463365
positive genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
positive genetic interaction witheif21translation initiation factor eIF2A (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcxr1mRNA processing factor Positive GeneticPMID:22681890
positive genetic interaction withtps1alpha,alpha-trehalose-phosphate synthase [UDP-forming] Positive GeneticPMID:22681890
positive genetic interaction withubp2ubiquitin C-terminal hydrolase Ubp2 Positive GeneticPMID:22681890
positive genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
positive genetic interaction withssm4dynactin microtubule-binding subunit, p150-Glued Ssm4 Positive GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
positive genetic interaction withmpr1histidine-containing response regulator phosphotransferase Mpr1 Positive GeneticPMID:22681890
negative genetic interaction withpmp1dual-specificity MAP kinase phosphatase Pmp1 Negative GeneticPMID:22681890
negative genetic interaction withbud32EKC/KEOPS protein complex associated ATPase Bud32 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsfp1transcription factor Sfp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmst2histone acetyltransferase Mst2 Negative GeneticPMID:22681890
negative genetic interaction withswr1SNF2 family ATP-dependent DNA helicase Swr1 Negative GeneticPMID:22681890
negative genetic interaction withrpb9DNA-directed RNA polymerase II complex subunit Rpb9 Negative GeneticPMID:22681890
negative genetic interaction withSPCC1919.13cribosome biogenesis protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmyp2myosin II heavy chain Myo3 Negative GeneticPMID:22681890
negative genetic interaction witharp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
negative genetic interaction withhrq1RecQ type DNA helicase Hrq1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withubr1N-end-recognizing protein, UBR ubiquitin-protein ligase E3 Ubr1 Negative GeneticPMID:22681890
negative genetic interaction withbun107WD repeat protein, human WDR48 family Bun107 Negative GeneticPMID:22681890
negative genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
negative genetic interaction withuch1ubiquitin C-terminal hydrolase Uch1 Negative GeneticPMID:22681890
negative genetic interaction withfml1ATP-dependent 3' to 5' DNA helicase, FANCM ortholog Fml1 Negative GeneticPMID:22681890
negative genetic interaction withmsc1Swr1 complex subunit Chk1 Negative GeneticPMID:22681890
negative genetic interaction withago1argonaute Negative GeneticPMID:22681890
negative genetic interaction withphp3CCAAT-binding factor complex subunit Php3 Negative GeneticPMID:22681890
negative genetic interaction withmtr4ATP-dependent RNA helicase, TRAMP complex subunit Mtr4 Negative GeneticPMID:22681890
negative genetic interaction withpst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
negative genetic interaction withSPCC16C4.106-phosphogluconolactonase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withchp1chromodomain protein Chp1 Negative GeneticPMID:22681890
negative genetic interaction withcwf2RNA-binding protein Cwf2 Negative GeneticPMID:22681890
negative genetic interaction withalp31tubulin specific chaperone cofactor A, Alp31 Negative GeneticPMID:22681890
negative genetic interaction withdcr1dicer Negative GeneticPMID:22681890
negative genetic interaction withspg1GTPase Spg1 Negative GeneticPMID:22681890
negative genetic interaction withbrr2U5 snRNP complex subunit Brr2 Negative GeneticPMID:22681890
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
negative genetic interaction withmug28RNA-binding protein Mug28 Negative GeneticPMID:22681890
negative genetic interaction withrdi1Rho GDP dissociation inhibitor Rdi1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsts5RNB-like protein Negative GeneticPMID:22681890
negative genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpar1protein phosphatase PP2A regulatory subunit B-56 Par1 Negative GeneticPMID:22681890
negative genetic interaction withcdc16two-component GAP Cdc16 Negative GeneticPMID:22681890
negative genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBPB10D8.02carylsulfatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withase1antiparallel microtubule cross-linking factor Ase1 Negative GeneticPMID:22681890
negative genetic interaction withcph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
negative genetic interaction withufd2ubiquitin-protein ligase E4 Ufd2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtip1CLIP170 family protein Tip1 Negative GeneticPMID:22681890
negative genetic interaction withreb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Negative GeneticPMID:22681890
negative genetic interaction withjmj2histone demethylase Jmj2 Negative GeneticPMID:22681890
negative genetic interaction withgid7GID complex subunit Gid7 (predicted) Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC13C5.01c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13C5.01c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13C5.01c BioGRID Interaction Datasets
Expression Viewer SPAC13C5.01c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13C5.01c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13C5.01c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13C5.01c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13C5.01c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13C5.01c Transcriptome Viewer (Bähler Lab)
GEO SPAC13C5.01c GEO profiles
PInt SPAC13C5.01c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13C5.01c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13C5.01c Fission yeast phenotypic data & analysis
Cyclebase SPAC13C5.01c.1 Cell Cycle Data
IntEnz3.4.25.1Integrated relational Enzyme database
Rhea3.4.25.1Annotated reactions database
SPD / RIKEN09/09A11Orfeome Localization Data
UniProtKB/SwissProtQ09682Probable proteasome subunit alpha type-3
ModBaseQ09682Database of comparative protein structure models
STRINGQ09682Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001712988972h- 20S proteasome component alpha 3 (predicted) (SPAC13C5.01c), mRNA
RefSeq PeptideXP_00171304020S proteasome component alpha 3 (predicted)
European Nucleotide ArchiveCU329670ENA EMBL mapping
MEROPST01.973MEROPS - the Peptidase Database
European Nucleotide ArchiveCAA90475ENA Protein Mapping
UniParcUPI0000132456UniProt Archive

Literature for pre9

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016