alp11 (SPAC13D6.05)


Gene Standard Namealp11 Characterisation Statuspublished
Systematic IDSPAC13D6.05 Feature Typeprotein coding
SynonymsSPAC4G9.01 Name Description
Producttubulin specific chaperone cofactor B Product Size234aa, 26.67 kDa
Genomic Location Chromosome I, 2253359-2254440 (1082nt); CDS:2253468-2254172 (705nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
alpha-tubulin binding2
Annotation ExtensionEvidenceWith/FromReference
protein binding831
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
microtubule cytoskeleton organization109
Annotation ExtensionEvidenceWith/FromReference
protein folding106
Annotation ExtensionEvidenceWith/FromReference
tubulin complex assembly20
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4205
Annotation ExtensionEvidenceWith/FromReference
cytoskeleton395
Annotation ExtensionEvidenceWith/FromReference
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
nucleus2740
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyalp11ΔNullPMID:204732891338
Microscopyalp11ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002481inviable branched, curved vegetative cellMicroscopyalp11ΔNullPECO:0000005, PECO:0000137PMID:236978067
FYPO:0002113inviable curved vegetative cellMicroscopyalp11ΔNullPECO:0000005, PECO:0000137PMID:2369780631
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
122533592254440

UTRs

Region Coordinates Reference
five_prime_UTR2253359..2253467PMID:21511999
three_prime_UTR2254173..2254440PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01302 Pfam IPR000938 CAP Gly-rich domain 155 221 3
PF14560 Pfam IPR027933 Ubiquitin-like domain 4 86 1
SM00213 SMART IPR000626 Ubiquitin domain 4 84 13
SM01052 SMART IPR000938 CAP Gly-rich domain 154 221 3
PS00845 Prosite Patterns IPR000938 CAP Gly-rich domain 172 205 3
PS50053 Prosite Profiles IPR000626 Ubiquitin domain 4 81 18
PS50245 Prosite Profiles IPR000938 CAP Gly-rich domain 174 216 3
PTHR18916 HMMPANTHER 3 231 3
2.30.30.190 Gene3D IPR000938 CAP Gly-rich domain 152 231 3
3.10.20.90 Gene3D 4 87 28
SSF54236 SuperFamily Ubiquitin-related domain 1 88 29
SSF74924 SuperFamily IPR000938 CAP Gly-rich domain 92 224 3

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001447ubiquitin family proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001447

Protein Properties

Ave. residue weight 113.98 Da
Charge -4.50
Isoelectric point 5.06
Molecular weight 26.67 kDa
Number of residues 234
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS213PMID:182575171670
present during cellular response to thiabendazole
experimental evidenceS213PMID:24763107
present during mitotic M phaseexperimental evidenceS213PMID:21712547
MOD:00696phosphorylated residueNASPMID:182575171922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
42055during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
45688during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
39581during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
44380during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
42261during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
8439.04during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
8341.03during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.7during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.81during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
atb2tubulin alpha 2 Affinity Capture-WesternPMID:10473641
nda2tubulin alpha 1 Co-fractionationPMID:10905343
nda3tubulin beta Nda3 Co-fractionationPMID:10905343
alp21tubulin specific chaperone cofactor E Affinity Capture-WesternPMID:10473641
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
atb2tubulin alpha 2 Dosage RescuePMID:10473641
alp1tubulin specific chaperone cofactor D, Alp1 Dosage RescuePMID:10683446
Dosage RescuePMID:10473641
alp21tubulin specific chaperone cofactor E Dosage RescuePMID:10683446
Dosage RescuePMID:10473641
External References
Database Identifier Description
NBRP SPAC13D6.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13D6.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13D6.05 BioGRID Interaction Datasets
Expression Viewer SPAC13D6.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13D6.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13D6.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13D6.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13D6.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13D6.05 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC13D6.05 Cell Cycle Data
GEO SPAC13D6.05 GEO profiles
PInt SPAC13D6.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13D6.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13D6.05 Fission yeast phenotypic data & analysis
SPD / RIKEN08/08C09Orfeome Localization Data
UniProtKB/SwissProtQ10235Cell polarity protein alp11
ModBaseQ10235Database of comparative protein structure models
STRINGQ10235Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593683tubulin specific chaperone cofactor B
RefSeq mRNANM_001019115972h- tubulin specific chaperone cofactor B (alp11), mRNA
European Nucleotide ArchiveBAA23375.1ENA Protein Mapping
European Nucleotide ArchiveCAB59431.1ENA Protein Mapping
UniParcUPI000012578FUniProt Archive

Literature for alp11

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014