aps1 (SPAC13G6.14)


Gene Standard Nameaps1 Characterisation Statuspublished
Systematic IDSPAC13G6.14 Feature Typeprotein coding
SynonymsSPAC24B11.03 Name DescriptionApsixA hydrolase
Productdiadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1 Product Size210aa, 23.72 kDa
Genomic Location Chromosome I, 200121-201517 (1397nt); CDS:200235-200867 (633nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0034431bis(5'-adenosyl)-hexaphosphatase activityIDAPMID:100907521
IMPPMID:12387729
GO:0034432bis(5'-adenosyl)-pentaphosphatase activityIDAPMID:100907521
GO:0008486diphosphoinositol-polyphosphate diphosphatase activityIEA EC:3.6.1.52GO_REF:00000031
GO:0052840inositol diphosphate tetrakisphosphate diphosphatase activityIEA EC:3.6.1.52GO_REF:00000031
GO:0052846inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activityIEA EC:3.6.1.52GO_REF:00000031
GO:0052847inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activityIEA EC:3.6.1.52GO_REF:00000031
GO:0052843inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activityIEA EC:3.6.1.52GO_REF:00000031
GO:0052848inositol-3,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activityIEA EC:3.6.1.52GO_REF:00000031
GO:0052844inositol-3-diphosphate-1,2,4,5,6-pentakisphosphate diphosphatase activityIEA EC:3.6.1.52GO_REF:00000031
GO:0052845inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activityIEA EC:3.6.1.52GO_REF:00000031
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037748
NOT GO:0047710bis(5'-adenosyl)-triphosphatase activityIDAPMID:10090752
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1901911adenosine 5'-(hexahydrogen pentaphosphate) catabolic processIDAPMID:100907521
GO:1901909diadenosine hexaphosphate catabolic processIMPPMID:123877291
IDAPMID:10090752
GO:1901907diadenosine pentaphosphate catabolic processIDAPMID:100907521
GO:1901906diadenosine pentaphosphate metabolic processIMPPMID:123877291
GO:0015959diadenosine polyphosphate metabolic processIDAPMID:100907523
GO:0071543diphosphoinositol polyphosphate metabolic processIMPPMID:123877291
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722319
GO:0005634nucleusIDAPMID:168233722737
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000637increased cell population growth rate on glucose carbon sourceCell growth assayaps1+ (wild type)OverexpressionPECO:0000126PMID:123877297
FYPO:0001420normal vegetative cell population growth rateCell growth assayaps1ΔNullPECO:0000103, PECO:0000126PMID:1238772944
Cell growth assayaps1ΔNullPECO:0000103, PECO:0000137PMID:12387729
Cell growth assayE93Q (E93Q)OverexpressionPECO:0000126PMID:12387729
FYPO:0000097sensitive to caffeine99
expressivity FYPO_EXT:0000001Cell growth assayaps1ΔNullPECO:0000137PMID:19672306
FYPO:0000087sensitive to hydrogen peroxide124
expressivity FYPO_EXT:0000003Cell growth assayaps1ΔNullPECO:0000137PMID:19672306
FYPO:0003612viable spore populationCell growth assayaps1ΔNullPMID:123877294
FYPO:0002060viable vegetative cell populationMicroscopyaps1ΔNullPECO:0000005, PECO:0000137PMID:236978063751
Microscopyaps1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003724decreased bis(5'-adenosyl)-hexaphosphatase activityEnzyme assay dataaps1ΔNullPMID:123877291
Enzyme assay dataE93Q (E93Q)OverexpressionPMID:12387729
FYPO:0003725increased bis(5'-adenosyl)-hexaphosphatase activityEnzyme assay dataaps1+ (wild type)OverexpressionPMID:123877291
FYPO:0003726increased cellular inositol pentakisphosphate levelSubstance quantification evidenceaps1ΔNullPMID:123877291
FYPO:0001494inviable elongated multiseptate vegetative cellMicroscopyaps1+ (wild type)OverexpressionPMID:1238772910
FYPO:0000021spheroid vegetative cellMicroscopyaps1+ (wild type)OverexpressionPMID:12387729113
FYPO:0002377viable swollen vegetative cellMicroscopyaps1+ (wild type)OverexpressionPMID:123877298
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopyE93Q (E93Q)OverexpressionPMID:123877293088
penetrance FYPO_EXT:0000001Microscopyaps1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopyaps1ΔNullPMID:12387729
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1200121201517
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00293 Pfam IPR000086 NUDIX hydrolase domain 42 195 8
PS00893 Prosite Patterns IPR020084 NUDIX hydrolase, conserved site 74 95 4
PS51462 Prosite Profiles IPR000086 NUDIX hydrolase domain 40 203 8
PTHR12629 HMMPANTHER 1 206 1
PTHR12629:SF0 HMMPANTHER 1 206 1
3.90.79.10 Gene3D IPR015797 NUDIX hydrolase domain-like 151 201 9
3.90.79.10 Gene3D IPR015797 NUDIX hydrolase domain-like 39 113 9
SSF55811 SuperFamily IPR015797 NUDIX hydrolase domain-like 164 200 9
SSF55811 SuperFamily IPR015797 NUDIX hydrolase domain-like 33 122 9

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.97 Da
Charge 8.50
Isoelectric point 10.14
Molecular weight 23.72 kDa
Number of residues 210
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Western blot evidencePMID:10090752

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
13845during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
15035during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
15432during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
15021during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2883.18during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
13760during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1732.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.3during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.62during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
vps35retromer complex subunit Vps35 Positive GeneticPMID:22681890
pnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:22681890
ppk9serine/threonine protein kinase Ppk9 (predicted) Negative GeneticPMID:22681890
ubp6ubiquitin C-terminal hydrolase Ubp6 Negative GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Positive GeneticPMID:22681890
usp102U1 snRNP-associated protein Usp102 Positive GeneticPMID:22681890
raf2Rik1-associated factor Raf2 Positive GeneticPMID:22681890
pof13F-box protein Pof13 Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Phenotypic SuppressionPMID:21169418
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
rho3Rho family GTPase Rho3 Dosage RescuePMID:21304827
dad2DASH complex subunit Dad2 Positive GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
pho7transcription factor Pho7 Phenotypic SuppressionPMID:21169418
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
pub3HECT-type ubiquitin-protein ligase E3 Pub3 (predicted) Negative GeneticPMID:22681890
spc34DASH complex subunit Spc34 Positive GeneticPMID:22681890
rpb7DNA-directed RNA polymerase complex II subunit Rpb7 Negative GeneticPMID:22681890
cdc10MBF transcription factor complex subunit Cdc10 Positive GeneticPMID:22681890
raf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Positive GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
SPBC17A3.03cphosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
SPAC19B12.11czinc finger protein, human ZNF593 ortholog Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rho3Rho family GTPase Rho3 Affinity Capture-WesternPMID:21304827
sip1Pof6 interacting protein Sip1, predicted AP-1 accessory protein Reconstituted ComplexPMID:23028933
Reconstituted ComplexPMID:23840894
External References
Database Identifier Description
NBRP SPAC13G6.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13G6.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13G6.14 BioGRID Interaction Datasets
Expression Viewer SPAC13G6.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13G6.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13G6.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13G6.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13G6.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13G6.14 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC13G6.14 Cell Cycle Data
GEO SPAC13G6.14 GEO profiles
PInt SPAC13G6.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13G6.14 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN07/07C02Orfeome Localization Data
IntEnz3.6.1.52Integrated relational Enzyme database
Rhea3.6.1.52Annotated reactions database
UniProtKB/SwissProtQ09790Diphosphoinositol polyphosphate phosphohydrolase aps1
ModBaseQ09790Database of comparative protein structure models
STRINGQ09790Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592840diadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1
RefSeq mRNANM_001018241972h- diadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1 (aps1), mRNA
European Nucleotide ArchiveAAD20015.1ENA Protein Mapping
European Nucleotide ArchiveCAA91107.1ENA Protein Mapping
UniParcUPI0000125CBAUniProt Archive

Literature for aps1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014