aps1 (SPAC13G6.14)


Gene Standard Nameaps1 Characterisation Statuspublished
Systematic IDSPAC13G6.14 Feature Typeprotein coding
SynonymsSPAC24B11.03 Name DescriptionApsixA hydrolase
Productdiadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1 Product Size210aa, 23.72 kDa
Genomic Location Chromosome I, 200121-201517 (1397nt); CDS:200235-200867 (633nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
bis(5'-adenosyl)-hexaphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
bis(5'-adenosyl)-pentaphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
diphosphoinositol-polyphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol diphosphate tetrakisphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-3,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-3-diphosphate-1,2,4,5,6-pentakisphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
metal ion binding747
Annotation ExtensionEvidenceWith/FromReference
NOT bis(5'-adenosyl)-triphosphatase activity
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
adenosine 5'-(hexahydrogen pentaphosphate) catabolic process1
Annotation ExtensionEvidenceWith/FromReference
diadenosine hexaphosphate catabolic process1
Annotation ExtensionEvidenceWith/FromReference
diadenosine pentaphosphate catabolic process1
Annotation ExtensionEvidenceWith/FromReference
diadenosine pentaphosphate metabolic process1
Annotation ExtensionEvidenceWith/FromReference
diadenosine polyphosphate metabolic process3
Annotation ExtensionEvidenceWith/FromReference
diphosphoinositol polyphosphate metabolic process1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
increased cell population growth rate on glucose carbon sourceaps1+ (wild type)Overexpression11
normal vegetative cell population growth rateaps1ΔNull57
E93Q (E93Q)Overexpression
sensitive to caffeine108
expressivity FYPO_EXT:0000001aps1ΔNull
sensitive to hydrogen peroxide137
expressivity FYPO_EXT:0000003aps1ΔNull
viable spore populationaps1ΔNull27
viable vegetative cell populationaps1ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
decreased bis(5'-adenosyl)-hexaphosphatase activityaps1ΔNull1
E93Q (E93Q)Overexpression
increased bis(5'-adenosyl)-hexaphosphatase activityaps1+ (wild type)Overexpression1
increased cellular inositol pentakisphosphate levelaps1ΔNull1
inviable elongated multiseptate vegetative cellaps1+ (wild type)Overexpression10
spheroid vegetative cellaps1+ (wild type)Overexpression119
viable swollen vegetative cellaps1+ (wild type)Overexpression8
viable vegetative cell with normal cell morphologyaps1ΔNull3092
penetrance FYPO_EXT:0000001aps1ΔNull
E93Q (E93Q)Overexpression
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1200121201517

UTRs

Region Coordinates Reference
three_prime_UTR200868..201517PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00293 Pfam IPR000086 NUDIX hydrolase domain 42 195 8
PS00893 Prosite Patterns IPR020084 NUDIX hydrolase, conserved site 74 95 4
PS51462 Prosite Profiles IPR000086 NUDIX hydrolase domain 40 203 8
3.90.79.10 Gene3D IPR015797 NUDIX hydrolase domain-like 39 113 9
3.90.79.10 Gene3D IPR015797 NUDIX hydrolase domain-like 151 201 9
SSF55811 SuperFamily IPR015797 NUDIX hydrolase domain-like 164 200 9
SSF55811 SuperFamily IPR015797 NUDIX hydrolase domain-like 33 122 9

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.97 Da
Charge 8.50
Isoelectric point 10.14
Molecular weight 23.72 kDa
Number of residues 210
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Western blot evidencePMID:10090752

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
13845during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
15035during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
15432during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
15021during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2883.18during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
13760during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1732.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.62during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
rho3Rho family GTPase Rho3 Affinity Capture-WesternPMID:21304827
sip1Pof6 interacting protein Sip1, predicted AP-1 accessory protein Reconstituted ComplexPMID:23028933
Reconstituted ComplexPMID:23840894
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
vps35retromer complex subunit Vps35 Positive GeneticPMID:22681890
pnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:22681890
ppk9serine/threonine protein kinase Ppk9 (predicted) Negative GeneticPMID:22681890
ubp6ubiquitin C-terminal hydrolase Ubp6 Negative GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Positive GeneticPMID:22681890
usp102U1 snRNP-associated protein Usp102 Positive GeneticPMID:22681890
mal3EB1 family Mal3 Synthetic RescuePMID:25254656
raf2Rik1-associated factor Raf2 Positive GeneticPMID:22681890
pof13F-box protein Pof13 Negative GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Phenotypic SuppressionPMID:21169418
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
rho3Rho family GTPase Rho3 Dosage RescuePMID:21304827
dad2DASH complex subunit Dad2 Positive GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
pho7transcription factor Pho7 Phenotypic SuppressionPMID:21169418
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
pub3HECT-type ubiquitin-protein ligase E3 Pub3 (predicted) Negative GeneticPMID:22681890
spc34DASH complex subunit Spc34 Positive GeneticPMID:22681890
rpb7DNA-directed RNA polymerase complex II subunit Rpb7 Negative GeneticPMID:22681890
cdc10MBF transcription factor complex subunit Cdc10 Positive GeneticPMID:22681890
raf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Positive GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
SPBC17A3.03cphosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
SPAC19B12.11czinc finger protein, human ZNF593 ortholog Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC13G6.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13G6.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13G6.14 BioGRID Interaction Datasets
Expression Viewer SPAC13G6.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13G6.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13G6.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13G6.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13G6.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13G6.14 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC13G6.14 Cell Cycle Data
GEO SPAC13G6.14 GEO profiles
PInt SPAC13G6.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13G6.14 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13G6.14 Fission yeast phenotypic data & analysis
SPD / RIKEN07/07C02Orfeome Localization Data
IntEnz3.6.1.52Integrated relational Enzyme database
Rhea3.6.1.52Annotated reactions database
UniProtKB/SwissProtQ09790Diphosphoinositol polyphosphate phosphohydrolase aps1
ModBaseQ09790Database of comparative protein structure models
STRINGQ09790Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592840diadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1
RefSeq mRNANM_001018241972h- diadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1 (aps1), mRNA
European Nucleotide ArchiveAAD20015.1ENA Protein Mapping
European Nucleotide ArchiveCAA91107.1ENA Protein Mapping
UniParcUPI0000125CBAUniProt Archive

Literature for aps1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015