aps1 (SPAC13G6.14)


Gene Standard Nameaps1 Characterisation Statuspublished
Systematic IDSPAC13G6.14 Feature Typeprotein coding
Synonyms Name DescriptionApsixA hydrolase,
Supressor of cut TWenty
Productdiadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1 Product Size210aa, 23.72 kDa
Genomic Location Chromosome I, 200121-201517 (1397nt); CDS:200235-200867 (633nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
bis(5'-adenosyl)-hexaphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
bis(5'-adenosyl)-pentaphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
diphosphoinositol-polyphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol diphosphate tetrakisphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-3,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-3-diphosphate-1,2,4,5,6-pentakisphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
metal ion binding750
Annotation ExtensionEvidenceWith/FromReference
NOT bis(5'-adenosyl)-triphosphatase activity
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
adenosine 5'-(hexahydrogen pentaphosphate) catabolic process1
Annotation ExtensionEvidenceWith/FromReference
diadenosine hexaphosphate catabolic process1
Annotation ExtensionEvidenceWith/FromReference
diadenosine pentaphosphate catabolic process1
Annotation ExtensionEvidenceWith/FromReference
diadenosine pentaphosphate metabolic process1
Annotation ExtensionEvidenceWith/FromReference
diadenosine polyphosphate metabolic process3
Annotation ExtensionEvidenceWith/FromReference
diphosphoinositol polyphosphate metabolic process1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
increased cell population growth rate on glucose carbon sourceaps1+12
normal vegetative cell population growth rateE93Q (E93Q)79
aps1Δ
sensitive to caffeine during vegetative growthaps1Δ110
sensitive to hydrogen peroxideaps1Δ153
viable spore populationaps1Δ46
viable vegetative cell populationaps1Δ3831

Cell Phenotype

Term NameGenotypesCount
decreased bis(5'-adenosyl)-hexaphosphatase activityaps1Δ1
E93Q (E93Q)
increased bis(5'-adenosyl)-hexaphosphatase activityaps1+1
increased cellular inositol pentakisphosphate levelaps1Δ1
increased phosphatase activityaps1Δ20
increased phosphatase activity during cellular response to adenine starvationaps1Δ4
increased phosphatase activity during cellular response to phosphate starvationaps1Δ2
increased RNA level during vegetative growth222
affecting pho84aps1Δ
affecting pho1aps1Δ
inviable elongated multiseptate vegetative cellaps1+9
spheroid vegetative cellaps1+125
viable swollen vegetative cellaps1+13
viable vegetative cell with normal cell morphologyaps1Δ3099
E93Q (E93Q)
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons200121..201517
5' UTR200121..200234AU008141
3' UTR200868..201517PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00293 Pfam IPR000086 NUDIX hydrolase domain 42 195 8
PS51462 Prosite Profiles IPR000086 NUDIX hydrolase domain 40 203 8
PS00893 Prosite Patterns IPR020084 NUDIX hydrolase, conserved site 74 95 4
PTHR12629 HMMPANTHER 5 114 1
PTHR12629 HMMPANTHER 146 202 1
PTHR12629:SF0 HMMPANTHER 5 114 1
PTHR12629:SF0 HMMPANTHER 146 202 1
3.90.79.10 Gene3D IPR015797 NUDIX hydrolase domain-like 39 113 9
3.90.79.10 Gene3D IPR015797 NUDIX hydrolase domain-like 151 201 9
SSF55811 SuperFamily IPR015797 NUDIX hydrolase domain-like 33 122 9
SSF55811 SuperFamily IPR015797 NUDIX hydrolase domain-like 164 200 9

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.97 Da
Charge 8.50
Codon Adaptation Index 0.40
Isoelectric point 10.14
Molecular weight 23.72 kDa
Number of residues 210
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Western blot evidencePMID:10090752

Quantitative Gene Expression

View graphical display of gene expression data for aps1 (SPAC13G6.14)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
13845during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
15035during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
15432during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
15021during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2883.18during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
13760during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1732.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.3during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.62during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3422
conserved in vertebrates3397
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPAC13G6.14 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturessfp47Ubp4 interactor Sfp47 Affinity Capture-MSPMID:20838651
affinity captured bycrp79poly(A) binding protein Crp79 Affinity Capture-RNAPMID:20531409
Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPAC13G6.14 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withSPBC409.17cDUF1769 family protein Negative GeneticPMID:22681890
negative genetic interaction withSPAC1039.06alanine racemase (predicted) Negative GeneticPMID:22681890
negative genetic interaction witharg12argininosuccinate synthase Arg12 Negative GeneticPMID:22681890
negative genetic interaction withoga1Stm1 homolog Oga1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC887.08Schizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withalm1medial ring protein Alm1 Negative GeneticPMID:22681890
negative genetic interaction withsgf29SAGA complex subunit Sgf29 Negative GeneticPMID:18818364
negative genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withhhp2serine/threonine protein kinase Hhp2 Negative GeneticPMID:22681890
negative genetic interaction withmsh6MutS protein homolog Negative GeneticPMID:22681890
negative genetic interaction withSPAC12B10.15cribonuclease H2 complex subunit (predicted) Negative GeneticPMID:22681890
negative genetic interaction withryh1GTPase Ryh1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC17H9.13cglutamate 5-kinase (predicted) Negative GeneticPMID:22681890
negative genetic interaction witharu1arginase Aru1 Negative GeneticPMID:22681890
negative genetic interaction withgpa2heterotrimeric G protein alpha-2 subunit Gpa2 Negative GeneticPMID:22681890
negative genetic interaction withmed6mediator complex subunit Med6 Negative GeneticPMID:18818364
negative genetic interaction withubp2ubiquitin C-terminal hydrolase Ubp2 Negative GeneticPMID:18818364
negative genetic interaction withlys3saccharopine dehydrogenase Lys3 Negative GeneticPMID:22681890
negative genetic interaction withemc1ER membrane protein complex subunit Emc1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpek1MAP kinase kinase Pek1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC4H3.06human C19orf60 homolog Negative GeneticPMID:22681890
negative genetic interaction withtls1splicing factor Tls1 Negative GeneticPMID:22681890
negative genetic interaction witheif21translation initiation factor eIF2A (predicted) Negative GeneticPMID:18818364
negative genetic interaction withrga8Rho-type GTPase activating protein Rga8 Negative GeneticPMID:22681890
negative genetic interaction withsle1eisosome assembly protein Seg1 Negative GeneticPMID:22681890
negative genetic interaction withspf1Set1C PHD Finger protein Spf1 Negative GeneticPMID:18818364
negative genetic interaction withlys12homoisocitrate dehydrogenase Lys12 Negative GeneticPMID:22681890
negative genetic interaction withSPBC1709.16caromatic ring-opening dioxygenase (predicted) Negative GeneticPMID:22681890
negative genetic interaction witharg6acetylglutamate synthase Arg6 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsib1ferrichrome synthetase Sib1 Negative GeneticPMID:22681890
negative genetic interaction withlys2homoaconitate hydratase Lys2 Negative GeneticPMID:22681890
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withcao1copper amine oxidase Cao1 Negative GeneticPMID:22681890
negative genetic interaction withvps1dynamin family protein Vps1 Negative GeneticPMID:22681890
negative genetic interaction withmet11methylenetetrahydrofolate reductase Met11 Negative GeneticPMID:22681890
negative genetic interaction withpcf3CAF assembly factor (CAF-1) complex subunit C, Pcf3 Negative GeneticPMID:22681890
negative genetic interaction withSPAC750.04cS. pombe specific 5Tm protein family Negative GeneticPMID:22681890
negative genetic interaction withspa2cell polarity protein Spa2 Negative GeneticPMID:22681890
negative genetic interaction withSPAC1A6.01chuman thyroid receptor interacting protein homolog, transcription coactivator (predicted) Negative GeneticPMID:22681890
negative genetic interaction withssr3SWI/SNF and RSC complex subunit Ssr3 Negative GeneticPMID:22681890
negative genetic interaction withSPAC29A4.17cmitochondrial FUN14 family protein involved in mitophagy Negative GeneticPMID:22681890
negative genetic interaction withsnz1pyridoxine biosynthesis protein Negative GeneticPMID:22681890
negative genetic interaction withmyp2myosin II heavy chain Myo3 Negative GeneticPMID:22681890
negative genetic interaction withmug96Schizosaccharomyces pombe specific protein Negative GeneticPMID:22681890
negative genetic interaction withrad55RecA family ATPase Rad55/Rhp55 Negative GeneticPMID:22681890
negative genetic interaction withexg1glucan 1,6-beta-glucosidase Exg1 Negative GeneticPMID:22681890
negative genetic interaction withset1histone lysine methyltransferase Set1 Negative GeneticPMID:18818364
negative genetic interaction withmug114Schizosaccharomyces pombe specific protein Mug114 Negative GeneticPMID:22681890
negative genetic interaction withmed20mediator complex subunit Med20 Negative GeneticPMID:18818364
negative genetic interaction withswd1Set1C complex subunit Swd1 Negative GeneticPMID:18818364
negative genetic interaction withSPAC105.02cankyrin repeat protein, human PPP1R16A ortholog Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:18818364
negative genetic interaction withpcf2CAF assembly factor (CAF-1) complex subunit B, Pcf2 Negative GeneticPMID:22681890
negative genetic interaction withmpc2mitochondrial pyruvate transmembrane transporter Mpc2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withyos9sensor for misfolded ER glycoproteins Yos9 (predicted) Negative GeneticPMID:22681890
negative genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:18818364
negative genetic interaction withSPAC644.13cRab GTPase binding (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsum2translation initiation inhibitor (predicted) Negative GeneticPMID:18818364
negative genetic interaction withSPAPB1A10.05Schizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
negative genetic interaction withSPAC11D3.03cconserved protein Negative GeneticPMID:22681890
negative genetic interaction withckb2CK2 family regulatory subunit Ckb2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withlys4homocitrate synthase Negative GeneticPMID:22681890
negative genetic interaction withSPBC1271.14glutamate N-acetyltransferase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmug117conserved fungal protein Mug117 Negative GeneticPMID:22681890
negative genetic interaction withswd3WD repeat protein Swd3 Negative GeneticPMID:22681890
negative genetic interaction withzhf1zinc ion transmembrane transporter Zhf1 Negative GeneticPMID:22681890
negative genetic interaction witharg11N-acetyl-gamma-glutamyl-phosphate reductase/acetylglutamate kinase Negative GeneticPMID:22681890
negative genetic interaction withSPAC10F6.15UPF0300 family protein 1 Negative GeneticPMID:22681890
negative genetic interaction withrqc1ribosome quality control complex (RQC) complex subunit Rqc1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC328.04AAA family ATPase, unknown biological role Negative GeneticPMID:22681890
positive genetic interaction withmal1maltase alpha-glucosidase Mal1 Positive GeneticPMID:22681890
positive genetic interaction withSPAC13G6.08Cdc20/Fizzy subfamily WD repeat protein Positive GeneticPMID:22681890
positive genetic interaction withcyp3cyclophilin family peptidyl-prolyl cis- trans isomerase Cyp3 Positive GeneticPMID:22681890
positive genetic interaction withsac12inositol polyphosphate phosphatase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsil1nucleotide exchange factor for the ER lumenal Hsp70 chaperone, Sil1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withpit1serine/threonine protein kinase, meiotic Pit1 Positive GeneticPMID:22681890
positive genetic interaction withtip1CLIP170 family protein Tip1 Positive GeneticPMID:22681890
positive genetic interaction withvsl1vacuolar SNARE Vsl1/Vam7 Positive GeneticPMID:22681890
positive genetic interaction withhrp3ATP-dependent DNA helicase Hrp3 Positive GeneticPMID:22681890
positive genetic interaction withmba1mitochondrial respiratory complex assembly protein Mba1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withskb5Shk1 kinase binding protein 5 Positive GeneticPMID:22681890
positive genetic interaction withrkr1RQC complex ubiquitin-protein ligase E3 Rkr1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withmug191alpha-1,6- mannanase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPAC1071.05S-adenosylmethionine-dependent methyltransferase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPAC212.08cS. pombe specific GPI anchored protein family 1 Positive GeneticPMID:22681890
positive genetic interaction withrsc4RSC complex subunit Rsc4 Positive GeneticPMID:22681890
phenotype enhanced byvps24ESCRT III complex subunit Vps24 Phenotypic EnhancementPMID:19547744
External References
Database Identifier Description
NBRP SPAC13G6.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13G6.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13G6.14 BioGRID Interaction Datasets
Expression Viewer SPAC13G6.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13G6.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13G6.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13G6.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13G6.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13G6.14 Transcriptome Viewer (Bähler Lab)
GEO SPAC13G6.14 GEO profiles
PInt SPAC13G6.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13G6.14 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13G6.14 Fission yeast phenotypic data & analysis
Cyclebase SPAC13G6.14.1 Cell Cycle Data
IntEnz3.6.1.52Integrated relational Enzyme database
Rhea3.6.1.52Annotated reactions database
SPD / RIKEN07/07C02Orfeome Localization Data
UniProtKB/SwissProtQ09790Diphosphoinositol polyphosphate phosphohydrolase aps1
ModBaseQ09790Database of comparative protein structure models
STRINGQ09790Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592840diadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1
RefSeq mRNANM_001018241972h- diadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1 (aps1), mRNA
European Nucleotide ArchiveAF125215ENA EMBL mapping
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveAAD20015ENA Protein Mapping
European Nucleotide ArchiveCAA91107ENA Protein Mapping
UniParcUPI0000125CBAUniProt Archive

Literature for aps1

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016