aps1 (SPAC13G6.14)


Gene Standard Nameaps1 Characterisation Statuspublished
Systematic IDSPAC13G6.14 Feature Typeprotein coding
SynonymsSPAC24B11.03 Name DescriptionApsixA hydrolase
Productdiadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1 Product Size210aa, 23.72 kDa
Genomic Location Chromosome I, 200121-201517 (1397nt); CDS:200235-200867 (633nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
bis(5'-adenosyl)-hexaphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
bis(5'-adenosyl)-pentaphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
diphosphoinositol-polyphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol diphosphate tetrakisphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 1-diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-1,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-1-diphosphate-2,3,4,5,6-pentakisphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-3,5-bisdiphosphate-2,3,4,6-tetrakisphosphate 5-diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-3-diphosphate-1,2,4,5,6-pentakisphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
inositol-5-diphosphate-1,2,3,4,6-pentakisphosphate diphosphatase activity1
Annotation ExtensionEvidenceWith/FromReference
metal ion binding743
Annotation ExtensionEvidenceWith/FromReference
NOT bis(5'-adenosyl)-triphosphatase activity
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
adenosine 5'-(hexahydrogen pentaphosphate) catabolic process1
Annotation ExtensionEvidenceWith/FromReference
diadenosine hexaphosphate catabolic process1
Annotation ExtensionEvidenceWith/FromReference
diadenosine pentaphosphate catabolic process1
Annotation ExtensionEvidenceWith/FromReference
diadenosine pentaphosphate metabolic process1
Annotation ExtensionEvidenceWith/FromReference
diadenosine polyphosphate metabolic process3
Annotation ExtensionEvidenceWith/FromReference
diphosphoinositol polyphosphate metabolic process1
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
increased cell population growth rate on glucose carbon sourceaps1+ (wild type)Overexpression12
normal vegetative cell population growth rateaps1ΔNull70
E93Q (E93Q)Overexpression
sensitive to caffeine during vegetative growthaps1ΔNull106
sensitive to hydrogen peroxideaps1ΔNull139
viable spore populationaps1ΔNull40
viable vegetative cell populationaps1ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
decreased bis(5'-adenosyl)-hexaphosphatase activityaps1ΔNull1
E93Q (E93Q)Overexpression
increased bis(5'-adenosyl)-hexaphosphatase activityaps1+ (wild type)Overexpression1
increased cellular inositol pentakisphosphate levelaps1ΔNull1
increased phosphatase activityaps1ΔNull19
increased phosphatase activity during cellular response to phosphate starvationaps1ΔNull2
increased RNA level during vegetative growth200
affecting pho84aps1ΔNull
affecting pho1aps1ΔNull
inviable elongated multiseptate vegetative cellaps1+ (wild type)Overexpression8
spheroid vegetative cellaps1+ (wild type)Overexpression123
viable swollen vegetative cellaps1+ (wild type)Overexpression10
viable vegetative cell with normal cell morphologyaps1ΔNull3094
E93Q (E93Q)Overexpression
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons200121..201517
mRNA200121..201517
5' UTR200121..200234AU008141
CDS200235..200867
3' UTR200868..201517PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00293 Pfam IPR000086 NUDIX hydrolase domain 42 195 8
PS00893 Prosite Patterns IPR020084 NUDIX hydrolase, conserved site 74 95 4
PS51462 Prosite Profiles IPR000086 NUDIX hydrolase domain 40 203 8
PTHR12629 HMMPANTHER 1 206 1
3.90.79.10 Gene3D IPR015797 NUDIX hydrolase domain-like 39 113 9
3.90.79.10 Gene3D IPR015797 NUDIX hydrolase domain-like 151 201 9
SSF55811 SuperFamily IPR015797 NUDIX hydrolase domain-like 164 200 9
SSF55811 SuperFamily IPR015797 NUDIX hydrolase domain-like 33 122 9

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.97 Da
Charge 8.50
Isoelectric point 10.14
Molecular weight 23.72 kDa
Number of residues 210
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Western blot evidencePMID:10090752

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
13845during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
15035during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
15432during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
15021during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
13760during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2883.18during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1732.38during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.62during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesrho3Rho family GTPase Rho3 Affinity Capture-WesternPMID:21304827
forms complex withsip1Pof6 interacting protein Sip1, predicted AP-1 accessory protein Reconstituted ComplexPMID:23028933
forms complex withsip1Pof6 interacting protein Sip1, predicted AP-1 accessory protein Reconstituted ComplexPMID:23840894
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withcdc10MBF transcription factor complex subunit Cdc10 Positive GeneticPMID:22681890
positive genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Positive GeneticPMID:22681890
positive genetic interaction withdad2DASH complex subunit Dad2 Positive GeneticPMID:22681890
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
synthetically rescuesmal3EB1 family Mal3 Synthetic RescuePMID:25254656
rescued bypho7transcription factor Pho7 Phenotypic SuppressionPMID:21169418
negative genetic interaction withpnk1DNA kinase/phosphatase Pnk1 Negative GeneticPMID:22681890
negative genetic interaction withpof13F-box protein Pof13 Negative GeneticPMID:22681890
negative genetic interaction withppk9serine/threonine protein kinase Ppk9 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withpub3HECT-type ubiquitin-protein ligase E3 Pub3 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withraf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Positive GeneticPMID:22681890
positive genetic interaction withraf2Rik1-associated factor Raf2 Positive GeneticPMID:22681890
rescued by overexpression ofrho3Rho family GTPase Rho3 Dosage RescuePMID:21304827
negative genetic interaction withrpb7DNA-directed RNA polymerase complex II subunit Rpb7 Negative GeneticPMID:22681890
rescued bysnf5SWI/SNF complex subunit Snf5 Phenotypic SuppressionPMID:21169418
negative genetic interaction withSPAC19B12.11czinc finger protein, human ZNF593 ortholog Negative GeneticPMID:22681890
negative genetic interaction withSPBC17A3.03cphosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
positive genetic interaction withspc34DASH complex subunit Spc34 Positive GeneticPMID:22681890
negative genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
negative genetic interaction withubp6ubiquitin C-terminal hydrolase Ubp6 Negative GeneticPMID:22681890
positive genetic interaction withusp102U1 snRNP-associated protein Usp102 Positive GeneticPMID:22681890
positive genetic interaction withvps35retromer complex subunit Vps35 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC13G6.14 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13G6.14 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13G6.14 BioGRID Interaction Datasets
Expression Viewer SPAC13G6.14 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13G6.14 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13G6.14 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13G6.14 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13G6.14 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13G6.14 Transcriptome Viewer (Bähler Lab)
GEO SPAC13G6.14 GEO profiles
PInt SPAC13G6.14 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13G6.14 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13G6.14 Fission yeast phenotypic data & analysis
Cyclebase SPAC13G6.14.1 Cell Cycle Data
SPD / RIKEN07/07C02Orfeome Localization Data
IntEnz3.6.1.52Integrated relational Enzyme database
Rhea3.6.1.52Annotated reactions database
UniProtKB/SwissProtQ09790Diphosphoinositol polyphosphate phosphohydrolase aps1
ModBaseQ09790Database of comparative protein structure models
STRINGQ09790Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_592840diadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1
RefSeq mRNANM_001018241972h- diadenosine 5',5'''-p1,p6-hexaphosphate hydrolase Aps1 (aps1), mRNA
European Nucleotide ArchiveAAD20015.1ENA Protein Mapping
European Nucleotide ArchiveCAA91107.1ENA Protein Mapping
UniParcUPI0000125CBAUniProt Archive

Literature for aps1

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015