ssa1 (SPAC13G7.02c)


Gene Standard Namessa1 Characterisation Statusbiological role inferred
Systematic IDSPAC13G7.02c Feature Typeprotein coding
Synonymshsp70 Name Description
Productheat shock protein Ssa1 (predicted) Product Size644aa, 70.14 kDa
Genomic Location Chromosome I, 2297981-2295580 (2402nt); CDS:2297753-2295819 (1935nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding557
Annotation ExtensionEvidenceWith/FromReference
ATPase activity238
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding42
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translation469
Annotation ExtensionEvidenceWith/FromReference
protein folding100
Annotation ExtensionEvidenceWith/FromReference
protein refolding8
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
sensitive to hydroxyureassa1Δ594
sensitive to methyl methanesulfonatessa1Δ331
sensitive to thiabendazolessa1Δ329
viable vegetative cell populationssa1Δ3850

Cell Phenotype

Term NameGenotypesCount
abnormal microtubule cytoskeleton morphology during mitotic interphasessa1Δ186
increased cellular protein aggregate levelssa1Δ3
normal protein level during cellular response to heat1
affecting hsp90ssa1Δ
affecting psi1ssa1Δ
viable vegetative cell with normal cell morphologyssa1Δ3102

FYPO Multi-allele Phenotypes

Cell Phenotype

Term NameGenotypes
decreased protein degradation during vegetative growth
affecting sdj1L169P (L169P), ssa1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in sdj1 glyoxylase III sdj1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2297981..2295580
mRNA2297981..2295580
5' UTR2297981..2297754PMID:21511999
CDS2297753..2295819
3' UTR2295818..2295580AU010458
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00012 Pfam IPR013126 Heat shock protein 70 family 5 610 8
PS00329 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 195 208 6
PS00297 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 7 14 5
PS01036 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 332 346 7
PTHR19375 HMMPANTHER 2 644 8
PTHR19375:SF140 HMMPANTHER 2 644 2
1.20.1270.10 Gene3D IPR029048 Heat shock protein 70kD, C-terminal domain 517 620 6
3.90.640.10 Gene3D Cell division protein FtsA 229 309 16
3.30.30.30 Gene3D Chaperone DnaK 53 113 6
3.30.420.40 Gene3D N-acetyl-D-glucosamine kinase 314 356 24
3.30.420.40 Gene3D N-acetyl-D-glucosamine kinase 199 228 24
2.60.34.10 Gene3D IPR029047 Heat shock protein 70kD, peptide-binding domain 390 516 7
3.30.420.40 Gene3D N-acetyl-D-glucosamine kinase 121 198 24
3.30.420.40 Gene3D N-acetyl-D-glucosamine kinase 4 52 24
SSF100934 SuperFamily IPR029048 Heat shock protein 70kD, C-terminal domain 535 617 7
SSF53067 SuperFamily 189 380 25
SSF100920 SuperFamily IPR029047 Heat shock protein 70kD, peptide-binding domain 384 541 7
SSF53067 SuperFamily 1 185 25
PR00301 PRINTS IPR013126 Heat shock protein 70 family 388 407 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 31 43 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 361 381 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 469 485 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 140 160 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 53 61 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 3 16 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 329 345 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 201 211 8

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.92 Da
Charge -16.00
Codon Adaptation Index 0.66
Isoelectric point 4.87
Molecular weight 70.14 kDa
Number of residues 644
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during cellular response to thiabendazoleS546
present during cellular response to thiabendazoleT417
present during cellular response to thiabendazoleS151
S151, S509, S542, S546
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S546 PMID:18257517
present during cellular response to thiabendazole IDA T417 PMID:18257517
present during cellular response to thiabendazole IDA S151 PMID:18257517
IDA S546 PMID:25720772
IDA S509 PMID:25720772
IDA S151 PMID:25720772
IDA S542 PMID:25720772
O-phospho-L-threonineT35, T36 1085
Annotation ExtensionEvidenceResidueReference
IDA T35 PMID:25720772
IDA T36 PMID:25720772
phosphorylated residue 2511
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK54, K157, K346, K456, K498, K505, K510 512
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence K346 PMID:26412298
mass spectrometry evidence K54 PMID:26412298
mass spectrometry evidence K456 PMID:26412298
mass spectrometry evidence K498 PMID:26412298
IDA PMID:26412298
mass spectrometry evidence K157 PMID:26412298
mass spectrometry evidence K505 PMID:26412298
mass spectrometry evidence K510 PMID:26412298
sumoylated lysine 174
Annotation ExtensionEvidenceResidueReference
IDA PMID:26404184
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0034605expression microarray evidencePMID:12529438

Quantitative Gene Expression

View graphical display of gene expression data for ssa1 (SPAC13G7.02c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
216245during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
188786during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
203997during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
264912during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
45675.5during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
202298during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
17452.58during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9.7during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
conserved in bacteria1005
conserved in metazoa3427
conserved in vertebrates3402
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC13G7.02c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byimp1importin alpha Affinity Capture-MSPMID:25963819
affinity captured bymas5DNAJ domain protein Mas5 (predicted) Affinity Capture-WesternPMID:24146635
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured byrad52DNA recombination protein, Rad51 mediator Rad52 (previously Rad22) Affinity Capture-MSPMID:23779158
affinity captured byslm9hira protein Slm9 Affinity Capture-MSPMID:20976105
affinity captured bycka1serine/threonine protein kinase Cka1 Affinity Capture-MSPMID:23462181
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captured byrad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:20231270
binds activation domain construct withfes1Hsp70 nucleotide exchange factor Fes1 (predicted) Two-hybridPMID:23695164
affinity capturesrad52DNA recombination protein, Rad51 mediator Rad52 (previously Rad22) Affinity Capture-WesternPMID:23779158
interacts withfes1Hsp70 nucleotide exchange factor Fes1 (predicted) PCAPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC13G7.02c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withsck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withsap114U2 snRNP subunit Sap114 Negative GeneticPMID:22681890
negative genetic interaction withsty1MAP kinase Sty1 Negative GeneticPMID:22681890
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:22681890
negative genetic interaction withpab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
negative genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
negative genetic interaction withtps1alpha,alpha-trehalose-phosphate synthase [UDP-forming] Negative GeneticPMID:22681890
negative genetic interaction withtsc2tuberin Negative GeneticPMID:22681890
negative genetic interaction withmcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
negative genetic interaction withSPAC2F3.11exopolyphosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Negative GeneticPMID:22681890
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
negative genetic interaction withppk29Ark1/Prk1 family protein kinase Ppk29 Negative GeneticPMID:22681890
negative genetic interaction withcwf19complexed with Cdc5 protein Cwf19 Negative GeneticPMID:22681890
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
negative genetic interaction withsnf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
negative genetic interaction withpsy2protein phosphatase PP4 complex subunit Psy2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction witharp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
negative genetic interaction withSPAC1B3.02ctranscription elongation factor, Elf1 family (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
negative genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgim3prefoldin subunit 4, Gim3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withusp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
negative genetic interaction withbun107WD repeat protein, human WDR48 family Bun107 Negative GeneticPMID:22681890
negative genetic interaction withcph2Clr6 histone deacetylase associated PHD protein Cph2 Negative GeneticPMID:22681890
negative genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withpht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
negative genetic interaction withsum2translation initiation inhibitor (predicted) Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withcwf28splicing factor Cwf28 Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
negative genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
negative genetic interaction withmkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
positive genetic interaction withste20Rictor homolog, Ste20 Positive GeneticPMID:22681890
positive genetic interaction withrsv2transcription factor Rsv2 Positive GeneticPMID:22681890
positive genetic interaction withsts5RNB-like protein Positive GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC13G7.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13G7.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13G7.02c BioGRID Interaction Datasets
Expression Viewer SPAC13G7.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13G7.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13G7.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13G7.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13G7.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13G7.02c Transcriptome Viewer (Bähler Lab)
GEO SPAC13G7.02c GEO profiles
PInt SPAC13G7.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13G7.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13G7.02c Fission yeast phenotypic data & analysis
Cyclebase SPAC13G7.02c.1 Cell Cycle Data
SPD / RIKEN40/40G05Orfeome Localization Data
UniProtKB/SwissProtQ10265Probable heat shock protein ssa1
ModBaseQ10265Database of comparative protein structure models
STRINGQ10265Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593704heat shock protein Ssa1 (predicted)
RefSeq mRNANM_001019135972h- heat shock protein Ssa1 (predicted) (ssa1), mRNA
ePDB3FP4The European PDB
PDB3FP4PDB
PDBsum3FP4PDBsum
European Nucleotide ArchiveCAA93590.1ENA Protein Mapping
UniParcUPI0000162053UniProt Archive

Literature for ssa1

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016