ssa1 (SPAC13G7.02c)


Gene Standard Namessa1 Characterisation Statusbiological_role_inferred
Systematic IDSPAC13G7.02c Feature Typeprotein coding
Synonyms Name Description
Productheat shock protein Ssa1 (predicted) Product Size644aa, 70.14 kDa
Genomic Location Chromosome I, 2297981-2295580 (2402nt); CDS:2297753-2295819 (1935nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATPase activity236
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding41
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translation459
Annotation ExtensionEvidenceWith/FromReference
protein folding103
Annotation ExtensionEvidenceWith/FromReference
protein refolding6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
sensitive to hydroxyureassa1ΔNull534
sensitive to methyl methanesulfonatessa1ΔNull237
sensitive to thiabendazolessa1ΔNull211
viable vegetative cell populationssa1ΔNull3795

Cell Phenotype

Term NameAlleleExpressionCount
abnormal microtubule cytoskeleton morphology during mitotic interphasessa1ΔNull186
increased cellular protein aggregate levelssa1ΔNull2
normal protein level during cellular response to heat1
affecting hsp90ssa1ΔNull
affecting psi1ssa1ΔNull
viable vegetative cell with normal cell morphologyssa1ΔNull3093
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2297981..2295580
mRNA2297981..2295580
5' UTR2297981..2297754PMID:21511999
CDS2297753..2295819
3' UTR2295818..2295580AU010458
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00012 Pfam IPR013126 Heat shock protein 70 family 5 610 8
PS00329 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 195 208 6
PS01036 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 332 346 7
PS00297 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 7 14 5
PTHR19375:SF142 HMMPANTHER 1 644 2
PTHR19375 HMMPANTHER 1 644 8
1.20.1270.10 Gene3D IPR029048 Heat shock protein 70kD, C-terminal domain 517 620 6
3.30.420.40 Gene3D 199 228 24
3.30.420.40 Gene3D 314 356 24
3.30.30.30 Gene3D 53 113 6
3.90.640.10 Gene3D 229 309 16
2.60.34.10 Gene3D IPR029047 Heat shock protein 70kD, peptide-binding domain 390 516 7
3.30.420.40 Gene3D 4 52 24
3.30.420.40 Gene3D 121 198 24
SSF100934 SuperFamily IPR029048 Heat shock protein 70kD, C-terminal domain 535 617 7
SSF53067 SuperFamily 1 185 25
SSF53067 SuperFamily 189 380 25
SSF100920 SuperFamily IPR029047 Heat shock protein 70kD, peptide-binding domain 384 541 7
PR00301 PRINTS IPR013126 Heat shock protein 70 family 201 211 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 469 485 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 140 160 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 53 61 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 329 345 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 3 16 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 388 407 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 361 381 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 31 43 8

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.92 Da
Charge -16.00
Isoelectric point 4.87
Molecular weight 70.14 kDa
Number of residues 644
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during cellular response to thiabendazoleS546
present during cellular response to thiabendazoleT417
present during cellular response to thiabendazoleS151
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S151 PMID:18257517
present during cellular response to thiabendazole IDA S546 PMID:18257517
present during cellular response to thiabendazole IDA T417 PMID:18257517
phosphorylated residue 1926
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0034605expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
216245during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
188786during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
203997during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
264912during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
45675.5during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
202298during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
17452.58during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9.7during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
3.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3430
conserved in vertebrates3405
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured bycka1serine/threonine protein kinase Cka1 Affinity Capture-MSPMID:23462181
binds activation domain construct withfes1Hsp70 nucleotide exchange factor Fes1 (predicted) Two-hybridPMID:23695164
affinity captured bymas5DNAJ domain protein Mas5 (predicted) Affinity Capture-WesternPMID:24146635
affinity captured byrad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:20231270
affinity capturesrad52DNA recombination protein, Rad51 mediator Rad52 (previously Rad22) Affinity Capture-WesternPMID:23779158
affinity captured byrad52DNA recombination protein, Rad51 mediator Rad52 (previously Rad22) Affinity Capture-MSPMID:23779158
affinity captured byslm9hira protein Slm9 Affinity Capture-MSPMID:20976105
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
negative genetic interaction witharp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
negative genetic interaction withbun107WD repeat protein, human WDR48 family Bun107 Negative GeneticPMID:22681890
negative genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withcph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
negative genetic interaction withcwf19complexed with Cdc5 protein Cwf19 Negative GeneticPMID:22681890
negative genetic interaction withcwf28splicing factor Cwf28 Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:22681890
negative genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
negative genetic interaction withgim3prefoldin subunit 4, Gim3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withmcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
negative genetic interaction withmkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
negative genetic interaction withpab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
negative genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
negative genetic interaction withpht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
negative genetic interaction withppk29Ark1/Prk1 family protein kinase Ppk29 Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withpsy2protein phosphatase PP4 complex subunit Psy2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
positive genetic interaction withrsv2transcription factor Rsv2 Positive GeneticPMID:22681890
negative genetic interaction withsap114U2 snRNP subunit Sap114 Negative GeneticPMID:22681890
negative genetic interaction withsck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
negative genetic interaction withsnf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
negative genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC1B3.02ctranscription elongation factor, Elf1 family (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC2F3.11exopolyphosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
positive genetic interaction withste20Rictor homolog, Ste20 Positive GeneticPMID:22681890
positive genetic interaction withsts5RNB-like protein Positive GeneticPMID:22681890
negative genetic interaction withsty1MAP kinase Sty1 Negative GeneticPMID:22681890
negative genetic interaction withsum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:22681890
negative genetic interaction withtps1alpha,alpha-trehalose-phosphate synthase Negative GeneticPMID:22681890
negative genetic interaction withtsc2tuberin Negative GeneticPMID:22681890
negative genetic interaction withusp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
negative genetic interaction withypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
negative genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC13G7.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13G7.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13G7.02c BioGRID Interaction Datasets
Expression Viewer SPAC13G7.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13G7.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13G7.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13G7.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13G7.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13G7.02c Transcriptome Viewer (Bähler Lab)
GEO SPAC13G7.02c GEO profiles
PInt SPAC13G7.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13G7.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13G7.02c Fission yeast phenotypic data & analysis
Cyclebase SPAC13G7.02c.1 Cell Cycle Data
SPD / RIKEN40/40G05Orfeome Localization Data
UniProtKB/SwissProtQ10265Probable heat shock protein ssa1
ModBaseQ10265Database of comparative protein structure models
STRINGQ10265Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593704heat shock protein Ssa1 (predicted)
RefSeq mRNANM_001019135972h- heat shock protein Ssa1 (predicted) (ssa1), mRNA
ePDB3FP4The European PDB
PDB3FP4PDB
PDBsum3FP4PDBsum
European Nucleotide ArchiveCAA93590.1ENA Protein Mapping
UniParcUPI0000162053UniProt Archive

Literature for ssa1

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015