ssa1 (SPAC13G7.02c)

Gene Standard Namessa1 Characterisation Statusbiological_role_inferred
Systematic IDSPAC13G7.02c Feature Typeprotein coding
Synonyms Name Description
Productheat shock protein Ssa1 (predicted) Product Size644aa, 70.14 kDa
Genomic Location Chromosome I, 2297981-2295580 (2402nt); CDS:2297753-2295819 (1935nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATPase activity232
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding42
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translation473
Annotation ExtensionEvidenceWith/FromReference
protein folding106
Annotation ExtensionEvidenceWith/FromReference
protein refolding6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000088sensitive to hydroxyureaCell growth assayssa1ΔNullPECO:0000137, PECO:0000005PMID:23173672514
FYPO:0000089sensitive to methyl methanesulfonateCell growth assayssa1ΔNullPECO:0000137, PECO:0000005PMID:23173672219
FYPO:0000091sensitive to thiabendazoleCell growth assayssa1ΔNullPECO:0000137, PECO:0000005PMID:23173672176
FYPO:0002060viable vegetative cell populationMicroscopyssa1ΔNullPECO:0000005, PECO:0000137PMID:236978063759

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003625abnormal microtubule cytoskeleton morphology during mitotic interphase186
penetrance FYPO_EXT:0000002experimental phenotypic evidencessa1ΔNullPECO:0000005, PECO:0000103, PECO:0000137DOI:10.1016/j.devcel.2014.09.005
FYPO:0003153normal protein level during cellular response to heat1
affecting psi1Microscopyssa1ΔNullPMID:24146635
affecting hsp90Microscopyssa1ΔNullPMID:24146635
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyssa1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00012 Pfam IPR013126 Heat shock protein 70 family 5 610 8
PS00297 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 7 14 5
PS01036 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 332 346 7
PS00329 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 195 208 6
PTHR19375 HMMPANTHER 1 642 8
3.30.420.40 Gene3D 4 52 24
3.30.420.40 Gene3D 121 198 24
3.90.640.10 Gene3D 229 309 16
1.20.1270.10 Gene3D 517 620 6 Gene3D 390 516 7 Gene3D 53 113 6
3.30.420.40 Gene3D 199 228 24
3.30.420.40 Gene3D 314 356 24
SSF53067 SuperFamily 1 185 25
SSF53067 SuperFamily 189 380 25
SSF100934 SuperFamily Heat shock protein 70kD, C-terminal domain 535 617 7
SSF100920 SuperFamily Heat shock protein 70kD, peptide-binding domain 384 541 7
PR00301 PRINTS IPR013126 Heat shock protein 70 family 3 16 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 140 160 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 329 345 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 388 407 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 469 485 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 361 381 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 201 211 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 31 43 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 53 61 8

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.92 Da
Charge -16.00
Isoelectric point 4.87
Molecular weight 70.14 kDa
Number of residues 644

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
present during cellular response to thiabendazole
present during cellular response to thiabendazoleIDAS546PMID:18257517
present during cellular response to thiabendazoleIDAS151PMID:18257517
MOD:00696phosphorylated residueNASPMID:195477441922
Gene Expression

Qualitative Gene Expression

RNA levelincreased during GO:0034605expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
216245during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
188786during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
203997during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
264912during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
202298during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
45675.5during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
17452.58during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9.7during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
3.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
fes1Hsp70 nucleotide exchange factor Fes1 (predicted) Two-hybridPMID:23695164
rad52DNA recombination protein Rad52 (previously Rad22) Affinity Capture-MSPMID:23779158
Affinity Capture-Western
mas5DNAJ domain protein Mas5 (predicted) Affinity Capture-WesternPMID:24146635
rad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:20231270
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
cka1serine/threonine protein kinase Cka1 Affinity Capture-MSPMID:23462181
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
slm9hira protein Slm9 Affinity Capture-MSPMID:20976105
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
cwf28splicing factor Cwf28 Negative GeneticPMID:22681890
cwf19complexed with Cdc5 protein Cwf19 Negative GeneticPMID:22681890
pab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
SPAC2F3.11exopolyphosphatase (predicted) Negative GeneticPMID:22681890
sck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
xap5xap-5-like protein Negative GeneticPMID:24957674
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
sty1MAP kinase Sty1 Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
SPAC227.05prefoldin subunit 4 (predicted) Negative GeneticPMID:22681890
ste20Rictor homolog, Ste20 Positive GeneticPMID:22681890
rsv2transcription factor Rsv2 Positive GeneticPMID:22681890
snf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
SPAC1B3.02ctranscription elongation factor, Elf1 family (predicted) Negative GeneticPMID:22681890
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
ptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
sre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
psy2protein phosphatase PP4 complex subunit Psy2 (predicted) Negative GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
cph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
sts5RNB-like protein Positive GeneticPMID:22681890
pht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
arp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
usp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
ppk29Ark1/Prk1 family protein kinase Ppk29 Negative GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
mcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
bun107WD repeat protein, human WDR48 family Bun107 Negative GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
tps1alpha,alpha-trehalose-phosphate synthase Negative GeneticPMID:22681890
mkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
sap114U2 snRNP subunit Sap114 Negative GeneticPMID:22681890
SPAC1952.06cspliceosomal complex subunit (predicted) Negative GeneticPMID:22681890
sum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:22681890
tsc2tuberin Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC13G7.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13G7.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13G7.02c BioGRID Interaction Datasets
Expression Viewer SPAC13G7.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13G7.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13G7.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13G7.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13G7.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13G7.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC13G7.02c Cell Cycle Data
GEO SPAC13G7.02c GEO profiles
PInt SPAC13G7.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13G7.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13G7.02c Fission yeast phenotypic data & analysis
SPD / RIKEN40/40G05Orfeome Localization Data
UniProtKB/SwissProtQ10265Probable heat shock protein ssa1
ModBaseQ10265Database of comparative protein structure models
STRINGQ10265Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593704heat shock protein Ssa1 (predicted)
RefSeq mRNANM_001019135972h- heat shock protein Ssa1 (predicted) (ssa1), mRNA
ePDB3FP4The European PDB
European Nucleotide ArchiveCAA93590.1ENA Protein Mapping
UniParcUPI0000162053UniProt Archive

Literature for ssa1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014