ssa1 (SPAC13G7.02c)


Gene Standard Namessa1 Characterisation Statusbiological role inferred
Systematic IDSPAC13G7.02c Feature Typeprotein coding
Synonyms Name Description
Productheat shock protein Ssa1 (predicted) Product Size644aa, 70.14 kDa
Genomic Location Chromosome I, 2297981-2295580 (2402nt); CDS:2297753-2295819 (1935nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
ATPase activity240
Annotation ExtensionEvidenceWith/FromReference
unfolded protein binding43
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translation469
Annotation ExtensionEvidenceWith/FromReference
protein folding102
Annotation ExtensionEvidenceWith/FromReference
protein refolding8
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
sensitive to hydroxyureassa1Δ548
sensitive to methyl methanesulfonatessa1Δ255
sensitive to thiabendazolessa1Δ264
viable vegetative cell populationssa1Δ3837

Cell Phenotype

Term NameGenotypesCount
abnormal microtubule cytoskeleton morphology during mitotic interphasessa1Δ186
increased cellular protein aggregate levelssa1Δ3
normal protein level during cellular response to heat1
affecting hsp90ssa1Δ
affecting psi1ssa1Δ
viable vegetative cell with normal cell morphologyssa1Δ3100

FYPO Multi-allele Phenotypes

Cell Phenotype

Term NameGenotypes
decreased protein degradation during vegetative growth
affecting sdj1L169P (L169P), ssa1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in sdj1 glyoxylase III sdj1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2297981..2295580
5' UTR2297981..2297754PMID:21511999
3' UTR2295818..2295580AU010458
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00012 Pfam IPR013126 Heat shock protein 70 family 5 610 8
PS00297 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 7 14 5
PS00329 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 195 208 6
PS01036 Prosite Patterns IPR018181 Heat shock protein 70, conserved site 332 346 7
PTHR19375:SF140 HMMPANTHER 2 644 2
PTHR19375 HMMPANTHER 2 644 8
3.30.30.30 Gene3D Heat shock protein 70 family 53 113 6
3.30.420.40 Gene3D Carbohydrate kinase, FGGY 199 228 24
3.30.420.40 Gene3D Carbohydrate kinase, FGGY 314 356 24
2.60.34.10 Gene3D IPR029047 Heat shock protein 70kD, peptide-binding domain 390 516 7
3.30.420.40 Gene3D Carbohydrate kinase, FGGY 121 198 24
3.30.420.40 Gene3D Carbohydrate kinase, FGGY 4 52 24
3.90.640.10 Gene3D Cell shape determining protein MreB/Mrl 229 309 16
1.20.1270.10 Gene3D IPR029048 Heat shock protein 70kD, C-terminal domain 517 620 6
SSF100934 SuperFamily IPR029048 Heat shock protein 70kD, C-terminal domain 535 617 7
SSF53067 SuperFamily 189 380 25
SSF100920 SuperFamily IPR029047 Heat shock protein 70kD, peptide-binding domain 384 541 7
SSF53067 SuperFamily 1 185 25
PR00301 PRINTS IPR013126 Heat shock protein 70 family 140 160 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 469 485 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 329 345 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 53 61 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 201 211 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 361 381 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 31 43 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 388 407 8
PR00301 PRINTS IPR013126 Heat shock protein 70 family 3 16 8

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.92 Da
Charge -16.00
Codon Adaptation Index 0.66
Isoelectric point 4.87
Molecular weight 70.14 kDa
Number of residues 644
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1676
present during cellular response to thiabendazoleS546
present during cellular response to thiabendazoleT417
present during cellular response to thiabendazoleS151
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S546 PMID:18257517
present during cellular response to thiabendazole IDA T417 PMID:18257517
present during cellular response to thiabendazole IDA S151 PMID:18257517
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK54, K157, K346, K456, K498, K505, K510 512
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence K456 PMID:26412298
mass spectrometry evidence K346 PMID:26412298
mass spectrometry evidence K498 PMID:26412298
mass spectrometry evidence K505 PMID:26412298
IDA PMID:26412298
mass spectrometry evidence K510 PMID:26412298
mass spectrometry evidence K157 PMID:26412298
mass spectrometry evidence K54 PMID:26412298
sumoylated lysine 173
Annotation ExtensionEvidenceResidueReference
IDA PMID:26404184
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0034605expression microarray evidencePMID:12529438

Quantitative Gene Expression

View graphical display of gene expression data for ssa1 (SPAC13G7.02c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
216245during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
188786during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
203997during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
264912during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
202298during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
45675.5during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
17452.58during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9.7during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4605
conserved in eukaryotes4515
conserved in bacteria1001
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC13G7.02c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withfes1Hsp70 nucleotide exchange factor Fes1 (predicted) Two-hybridPMID:26771498
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captured byslm9hira protein Slm9 Affinity Capture-MSPMID:20976105
affinity captured byrad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:20231270
affinity captured byrad52DNA recombination protein, Rad51 mediator Rad52 (previously Rad22) Affinity Capture-MSPMID:23779158
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured bycka1serine/threonine protein kinase Cka1 Affinity Capture-MSPMID:23462181
affinity captured bymas5DNAJ domain protein Mas5 (predicted) Affinity Capture-WesternPMID:24146635
affinity captured byimp1importin alpha Affinity Capture-MSPMID:25963819
affinity capturesrad52DNA recombination protein, Rad51 mediator Rad52 (previously Rad22) Affinity Capture-WesternPMID:23779158
interacts withfes1Hsp70 nucleotide exchange factor Fes1 (predicted) PCAPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC13G7.02c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
negative genetic interaction withsck2serine/threonine protein kinase Sck2 Negative GeneticPMID:24463365
negative genetic interaction withcph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
negative genetic interaction withpht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
negative genetic interaction withtps1alpha,alpha-trehalose-phosphate synthase [UDP-forming] Negative GeneticPMID:22681890
negative genetic interaction withphp5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:21504829
negative genetic interaction withsum2translation initiation inhibitor (predicted) Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withcwf28splicing factor Cwf28 Negative GeneticPMID:22681890
negative genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgim3prefoldin subunit 4, Gim3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
negative genetic interaction withcwf19complexed with Cdc5 protein Cwf19 Negative GeneticPMID:22681890
negative genetic interaction witharp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
negative genetic interaction withtsc2tuberin Negative GeneticPMID:22681890
negative genetic interaction withptc1protein phosphatase 2C Ptc1 Negative GeneticPMID:22681890
negative genetic interaction withmcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withSPAC1B3.02ctranscription elongation factor, Elf1 family (predicted) Negative GeneticPMID:22681890
negative genetic interaction withsap114U2 snRNP subunit Sap114 Negative GeneticPMID:22681890
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
negative genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
negative genetic interaction withsnf5SWI/SNF complex subunit Snf5 Negative GeneticPMID:22681890
negative genetic interaction withusp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withpsy2protein phosphatase PP4 complex subunit Psy2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC2F3.11exopolyphosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withppk29Ark1/Prk1 family protein kinase Ppk29 Negative GeneticPMID:22681890
negative genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withbun107WD repeat protein, human WDR48 family Bun107 Negative GeneticPMID:22681890
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withmkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Negative GeneticPMID:22681890
negative genetic interaction withsty1MAP kinase Sty1 Negative GeneticPMID:22681890
positive genetic interaction withsts5RNB-like protein Positive GeneticPMID:22681890
positive genetic interaction withste20Rictor homolog, Ste20 Positive GeneticPMID:22681890
positive genetic interaction withrsv2transcription factor Rsv2 Positive GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC13G7.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13G7.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13G7.02c BioGRID Interaction Datasets
Expression Viewer SPAC13G7.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13G7.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13G7.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13G7.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13G7.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13G7.02c Transcriptome Viewer (Bähler Lab)
GEO SPAC13G7.02c GEO profiles
PInt SPAC13G7.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13G7.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13G7.02c Fission yeast phenotypic data & analysis
Cyclebase SPAC13G7.02c.1 Cell Cycle Data
SPD / RIKEN40/40G05Orfeome Localization Data
UniProtKB/SwissProtQ10265Probable heat shock protein ssa1
ModBaseQ10265Database of comparative protein structure models
STRINGQ10265Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593704heat shock protein Ssa1 (predicted)
RefSeq mRNANM_001019135972h- heat shock protein Ssa1 (predicted) (ssa1), mRNA

Literature for ssa1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016