met16 (SPAC13G7.06)


Gene Standard Namemet16 Characterisation Statuspublished
Systematic IDSPAC13G7.06 Feature Typeprotein coding
Synonyms Name Description
Productphosphoadenosine phosphosulfate reductase Product Size266aa, 30.56 kDa
Genomic Location Chromosome I, 2307438-2308878 (1441nt); CDS:2307729-2308529 (801nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
phosphoadenylyl-sulfate reductase (thioredoxin) activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytoplasm4209
Annotation ExtensionEvidenceWith/FromReference
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
abolished cell population growth on glycerol carbon sourcemet16Δ68
decreased vegetative cell population growthmet16Δ634
growth auxotrophic for cysteinemet16 unnamed mutant9
growth auxotrophic for methioninemet16 unnamed mutant10
met16Δ
growth auxotrophic for sulfur-containing amino acidmet16Δ15
sensitive to bortezomibmet16Δ255
sensitive to cadmiummet16Δ249
sensitive to hydrogen peroxidemet16Δ153
viable vegetative cell populationmet16Δ3831

Cell Phenotype

Term NameGenotypesCount
decreased phosphatase activity during cellular response to phosphate starvationmet16Δ11
viable vegetative cell with normal cell morphologymet16Δ3099
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2307438..2308878
5' UTR2307438..2307728PMID:21511999
3' UTR2308530..2308878PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01507 Pfam IPR002500 Phosphoadenosine phosphosulphate reductase 49 221 2
PTHR23293:SF11 HMMPANTHER 20 266 1
PTHR23293 HMMPANTHER 20 266 2
3.40.50.620 Gene3D IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 13 217 39
SSF52402 SuperFamily 22 209 16
PIRSF000857 PIRSF IPR004511 Phosphoadenosine phosphosulphate/adenosine 5'-phosphosulphate reductase 3 250 1
TIGR00434 tigrfam IPR004511 Phosphoadenosine phosphosulphate/adenosine 5'-phosphosulphate reductase 34 244 1
TIGR02057 tigrfam IPR011800 Phosphoadenosine phosphosulphate reductase CysH 22 244 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.89 Da
Charge 3.50
Codon Adaptation Index 0.46
Isoelectric point 7.30
Molecular weight 30.56 kDa
Number of residues 266
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for met16 (SPAC13G7.06)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
78465during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
83846during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
90308during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
78796during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
16893.06during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
82113during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8183.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6.5during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.99during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3422
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPAC13G7.06 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byago1argonaute Affinity Capture-MSPMID:24095277
Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPAC13G7.06 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withssu72phosphoric ester hydrolase Ssu72 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC1223.01ubiquitin-protein ligase E3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withtos4FHA domain protein Tos4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC513.07flavonol reductase/cinnamoyl-CoA reductase family Negative GeneticPMID:22681890
negative genetic interaction withsap14U2 snRNP-associated protein SF3B14 Sap14 Negative GeneticPMID:22681890
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
negative genetic interaction withrhp14XP-A family homolog Rhp14 Negative GeneticPMID:22681890
negative genetic interaction withngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
negative genetic interaction withcph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
negative genetic interaction withSPCC18.10pyridoxine-pyridoxal-pyridoxamine kinase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withcds1replication checkpoint kinase Cds1 Negative GeneticPMID:22681890
negative genetic interaction withppa2serine/threonine protein phosphatase Ppa2 Negative GeneticPMID:22681890
synthetic growth defect withdis2serine/threonine protein phosphatase PP1, Dis2 Synthetic Growth DefectPMID:21965289
overexpression rescuescwf14G10 protein Dosage RescuePMID:24874881
synthetically rescued bymst2histone acetyltransferase Mst2 Synthetic RescuePMID:21289066
rescuescwf14G10 protein Phenotypic SuppressionPMID:24874881
positive genetic interaction withelp3elongator complex subunit Elp3 (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC13G7.06 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13G7.06 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13G7.06 BioGRID Interaction Datasets
Expression Viewer SPAC13G7.06 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13G7.06 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13G7.06 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13G7.06 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13G7.06 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13G7.06 Transcriptome Viewer (Bähler Lab)
GEO SPAC13G7.06 GEO profiles
PInt SPAC13G7.06 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13G7.06 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13G7.06 Fission yeast phenotypic data & analysis
Cyclebase SPAC13G7.06.1 Cell Cycle Data
SPD / RIKEN43/43C07Orfeome Localization Data
IntEnz1.8.4.8Integrated relational Enzyme database
Rhea1.8.4.8Annotated reactions database
UniProtKB/SwissProtQ10270Probable phosphoadenosine phosphosulfate reductase
ModBaseQ10270Database of comparative protein structure models
STRINGQ10270Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593708phosphoadenosine phosphosulfate reductase
RefSeq mRNANM_001019139972h- phosphoadenosine phosphosulfate reductase (met16), mRNA
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveCAA93594ENA Protein Mapping
KEGG00920+1.8.4.8Sulfur metabolism
UniParcUPI000012F6C0UniProt Archive
UniPathwayUPA00140with

Literature for met16

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016