met16 (SPAC13G7.06)


Gene Standard Namemet16 Characterisation Statuspublished
Systematic IDSPAC13G7.06 Feature Typeprotein coding
Synonyms Name Description
Productphosphoadenosine phosphosulfate reductase Product Size266aa, 30.56 kDa
Genomic Location Chromosome I, 2307438-2308878 (1441nt); CDS:2307729-2308529 (801nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
phosphoadenylyl-sulfate reductase (thioredoxin) activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytoplasm4209
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
abolished cell population growth on glycerol carbon sourcemet16ΔNull67
decreased vegetative cell population growthmet16ΔNull571
growth auxotrophic for cysteinemet16 unnamed mutantNot specified9
growth auxotrophic for methioninemet16 unnamed mutantNot specified10
met16ΔNull
growth auxotrophic for sulfur-containing amino acidmet16ΔNull15
sensitive to bortezomibmet16ΔNull256
sensitive to cadmiummet16ΔNull246
sensitive to hydrogen peroxidemet16ΔNull139
viable vegetative cell populationmet16ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
decreased phosphatase activity during cellular response to phosphate starvationmet16ΔNull10
viable vegetative cell with normal cell morphologymet16ΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2307438..2308878
mRNA2307438..2308878
5' UTR2307438..2307728PMID:21511999
CDS2307729..2308529
3' UTR2308530..2308878PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01507 Pfam IPR002500 Phosphoadenosine phosphosulphate reductase 49 221 2
PTHR23293:SF5 HMMPANTHER 20 264 1
PTHR23293 HMMPANTHER 20 264 2
3.40.50.620 Gene3D IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 13 217 39
SSF52402 SuperFamily 22 209 16
PIRSF000857 PIRSF IPR004511 Phosphoadenosine phosphosulphate/adenosine 5'-phosphosulphate reductase 3 250 1
TIGR02057 tigrfam IPR011800 Phosphoadenosine phosphosulphate reductase CysH 22 244 1
TIGR00434 tigrfam IPR004511 Phosphoadenosine phosphosulphate/adenosine 5'-phosphosulphate reductase 34 244 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.89 Da
Charge 3.50
Isoelectric point 7.30
Molecular weight 30.56 kDa
Number of residues 266
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
78465during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
83846during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
90308during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
78796during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
82113during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
16893.06during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
8183.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6.5during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.99during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in bacteria1001
conserved in metazoa3424
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
negative genetic interaction withaut12autophagy associated protein Aut12 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
negative genetic interaction withmpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
negative genetic interaction withngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
positive genetic interaction withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
negative genetic interaction withpob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
positive genetic interaction withppk16serine/threonine protein kinase Ppk16 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
positive genetic interaction withrpa12DNA-directed RNA polymerase complex I subunit Rpa12 Positive GeneticPMID:22681890
rescuesSPBC713.07cvacuolar polyphosphatase (predicted) Phenotypic SuppressionPMID:21169418
positive genetic interaction withsty1MAP kinase Sty1 Positive GeneticPMID:22681890
positive genetic interaction withswc5Swr1 complex subunit Swc5 Positive GeneticPMID:22681890
negative genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC13G7.06 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13G7.06 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13G7.06 BioGRID Interaction Datasets
Expression Viewer SPAC13G7.06 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13G7.06 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13G7.06 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13G7.06 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13G7.06 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13G7.06 Transcriptome Viewer (Bähler Lab)
GEO SPAC13G7.06 GEO profiles
PInt SPAC13G7.06 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13G7.06 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13G7.06 Fission yeast phenotypic data & analysis
Cyclebase SPAC13G7.06.1 Cell Cycle Data
SPD / RIKEN43/43C07Orfeome Localization Data
IntEnz1.8.4.8Integrated relational Enzyme database
Rhea1.8.4.8Annotated reactions database
UniProtKB/SwissProtQ10270Probable phosphoadenosine phosphosulfate reductase
ModBaseQ10270Database of comparative protein structure models
STRINGQ10270Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593708phosphoadenosine phosphosulfate reductase
RefSeq mRNANM_001019139972h- phosphoadenosine phosphosulfate reductase (met16), mRNA
European Nucleotide ArchiveCAA93594.1ENA Protein Mapping
KEGG_Enzyme00920+1.8.4.8Sulfur metabolism
UniParcUPI000012F6C0UniProt Archive
UniPathwayUPA00140Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1

Literature for met16

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015