met16 (SPAC13G7.06)

Gene Standard Namemet16 Characterisation Statuspublished
Systematic IDSPAC13G7.06 Feature Typeprotein coding
Synonyms Name Description
Productphosphoadenosine phosphosulfate reductase Product Size266aa, 30.56 kDa
Genomic Location Chromosome I, 2307438-2308878 (1441nt); CDS:2307729-2308529 (801nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
phosphoadenylyl-sulfate reductase (thioredoxin) activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
abolished cell population growth on glycerol carbon sourcemet16ΔNull65
decreased vegetative cell population growthmet16ΔNull400
growth auxotrophic for cysteinemet16 unnamed mutantNot specified10
growth auxotrophic for methioninemet16 unnamed mutantNot specified10
growth auxotrophic for sulfur-containing amino acidmet16ΔNull16
sensitive to bortezomib256
expressivity FYPO_EXT:0000002met16ΔNull
sensitive to cadmium246
expressivity FYPO_EXT:0000001met16ΔNull
sensitive to hydrogen peroxidemet16ΔNull137
viable vegetative cell populationmet16ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphology3092
penetrance FYPO_EXT:0000001met16ΔNull
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01507 Pfam IPR002500 Phosphoadenosine phosphosulphate reductase 49 221 2
PTHR23293:SF5 HMMPANTHER 20 264 1
PTHR23293 HMMPANTHER 20 264 2 Gene3D IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 13 217 39
SSF52402 SuperFamily 22 209 16
PIRSF000857 PIRSF IPR004511 Phosphoadenosine phosphosulphate/adenosine 5'-phosphosulphate reductase 1 247 1
TIGR00434 tigrfam IPR004511 Phosphoadenosine phosphosulphate/adenosine 5'-phosphosulphate reductase 34 244 1
TIGR02057 tigrfam IPR011800 Phosphoadenosine phosphosulphate reductase CysH 22 244 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.89 Da
Charge 3.50
Isoelectric point 7.30
Molecular weight 30.56 kDa
Number of residues 266
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
78465during GO:0000080PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
83846during GO:0000084PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
90308during GO:0000085PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
78796during GO:0000087PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
16893.06during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
82113during GO:0072690PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
8183.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6.5during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
0.99during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3432

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
cwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
ppk16serine/threonine protein kinase Ppk16 (predicted) Positive GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
swc5Swr1 complex subunit Swc5 Positive GeneticPMID:22681890
pob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
mpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Positive GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
SPBC713.07cvacuolar polyphosphatase (predicted) Phenotypic SuppressionPMID:21169418
aut12autophagy associated protein Aut12 (predicted) Negative GeneticPMID:22681890
rpa12DNA-directed RNA polymerase complex I subunit Rpa12 Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC13G7.06 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13G7.06 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13G7.06 BioGRID Interaction Datasets
Expression Viewer SPAC13G7.06 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13G7.06 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13G7.06 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13G7.06 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13G7.06 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13G7.06 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC13G7.06 Cell Cycle Data
GEO SPAC13G7.06 GEO profiles
PInt SPAC13G7.06 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC13G7.06 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC13G7.06 Fission yeast phenotypic data & analysis
SPD / RIKEN43/43C07Orfeome Localization Data
IntEnz1.8.4.8Integrated relational Enzyme database
Rhea1.8.4.8Annotated reactions database
UniProtKB/SwissProtQ10270Probable phosphoadenosine phosphosulfate reductase
ModBaseQ10270Database of comparative protein structure models
STRINGQ10270Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593708phosphoadenosine phosphosulfate reductase
RefSeq mRNANM_001019139972h- phosphoadenosine phosphosulfate reductase (met16), mRNA
European Nucleotide ArchiveCAA93594.1ENA Protein Mapping
KEGG_Enzyme00920+ metabolism
UniParcUPI000012F6C0UniProt Archive
UniPathwayUPA00140Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1

Literature for met16

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015