met16 (SPAC13G7.06)


Gene Standard Namemet16 Characterisation Statuspublished
Systematic IDSPAC13G7.06 Feature Typeprotein coding
Synonyms Name Description
Productphosphoadenosine phosphosulfate reductase Product Size266aa, 30.56 kDa
Genomic Location Chromosome I, 2307438-2308878 (1441nt); CDS:2307729-2308529 (801nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004604phosphoadenylyl-sulfate reductase (thioredoxin) activityISOSGD:S000006371GO_REF:00000241
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0019344cysteine biosynthetic processIEAUniProtKB-KW:KW-0198GO_REF:000003711
GO:0070814hydrogen sulfide biosynthetic processIEA UniPathway:UPA00140GO_REF:00000419
GO:0009086methionine biosynthetic processIMPPMID:1643642830
GO:0019379sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin)ISOSGD:S000006371GO_REF:00000242
GO:0000096sulfur amino acid metabolic processIMPPMID:1762253340
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISOSGD:S000006371GO_REF:00000244201
GO:0005829cytosolIDAPMID:168233722316
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001934abolished cell population growth on glycerol carbon sourceCell growth assaymet16ΔNullPECO:0000072PMID:1866526861
FYPO:0001355decreased vegetative cell population growthCell growth assaymet16ΔNullPECO:0000126PMID:18665268305
FYPO:0000037growth auxotrophic for cysteineCell growth assaymet16 unnamed mutantNot specifiedPECO:0000126, PECO:0000005, PECO:0000102PMID:176225339
FYPO:0000040growth auxotrophic for methionineCell growth assaymet16 unnamed mutantNot specifiedPECO:0000126, PECO:0000005, PECO:0000102PMID:1762253310
Cell growth assaymet16ΔNullPECO:0000102, PECO:0000126PMID:16436428
FYPO:0003355growth auxotrophic for sulfur-containing amino acidCell growth assaymet16ΔNullPECO:0000178PMID:241180961
Cell growth assaymet16ΔNullPECO:0000179PMID:24118096
Cell growth assaymet16ΔNullPECO:0000118PMID:24118096
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000002Cell growth assaymet16ΔNullPECO:0000005, PECO:0000137PMID:21760946
FYPO:0000096sensitive to cadmium246
expressivity FYPO_EXT:0000001cell growth assaymet16ΔNullPECO:0000102, PECO:0000005, PECO:0000137PMID:18684775
FYPO:0000087sensitive to hydrogen peroxideCell growth assaymet16ΔNullPECO:0000137PMID:18665268121
FYPO:0002060viable vegetative cell populationMicroscopymet16ΔNullPECO:0000005, PECO:0000137PMID:236978063760
Microscopymet16ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3086
penetrance FYPO_EXT:0000001Microscopymet16ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
123074382308878

UTRs

Region Coordinates Reference
five_prime_UTR2307438..2307728PMID:21511999
three_prime_UTR2308530..2308878PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01507 Pfam IPR002500 Phosphoadenosine phosphosulphate reductase 49 221 2
PTHR23293 HMMPANTHER 20 264 2
PTHR23293:SF5 HMMPANTHER 20 264 1
3.40.50.620 Gene3D IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 13 217 39
SSF52402 SuperFamily 22 209 16
PIRSF000857 PIRSF IPR004511 Phosphoadenosine phosphosulphate/adenosine 5'-phosphosulphate reductase 1 247 1
TIGR00434 tigrfam IPR004511 Phosphoadenosine phosphosulphate/adenosine 5'-phosphosulphate reductase 34 244 1
TIGR02057 tigrfam IPR011800 Phosphoadenosine phosphosulphate reductase CysH 22 244 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.89 Da
Charge 3.50
Isoelectric point 7.30
Molecular weight 30.56 kDa
Number of residues 266
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
90308during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
78465during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
78796during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
82113during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
83846during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
8183.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
16893.06during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
6.5during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.99during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Positive GeneticPMID:22681890
ppk16serine/threonine protein kinase Ppk16 (predicted) Positive GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
swc5Swr1 complex subunit Swc5 Positive GeneticPMID:22681890
pob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
mpr1histidine-containing response regulator phosphotransferase Mpr1 Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Positive GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
SPBC713.07cvacuolar polyphosphatase (predicted) Phenotypic SuppressionPMID:21169418
aut12autophagy associated protein Aut12 (predicted) Negative GeneticPMID:22681890
rpa12DNA-directed RNA polymerase complex I subunit Rpa12 Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Positive GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
cwf2RNA-binding protein Cwf2 Affinity Capture-MSPMID:21386897
External References
Database Identifier Description
NBRP SPAC13G7.06 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC13G7.06 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC13G7.06 BioGRID Interaction Datasets
Expression Viewer SPAC13G7.06 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC13G7.06 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC13G7.06 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC13G7.06 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC13G7.06 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC13G7.06 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC13G7.06 Cell Cycle Data
GEO SPAC13G7.06 GEO profiles
PInt SPAC13G7.06 Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz1.8.4.8Integrated relational Enzyme database
Rhea1.8.4.8Annotated reactions database
WikiGene2542836phosphoadenosine phosphosulfate reductase
EntrezGene2542836phosphoadenosine phosphosulfate reductase
SPD / RIKEN43/43C07Orfeome Localization Data
UniProtKB/SwissProtQ10270Probable phosphoadenosine phosphosulfate reductase
ModBaseQ10270Database of comparative protein structure models
STRINGQ10270Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593708phosphoadenosine phosphosulfate reductase
RefSeq mRNANM_001019139972h- phosphoadenosine phosphosulfate reductase (met16), mRNA
European Nucleotide ArchiveCAA93594ENA Protein Mapping
European Nucleotide ArchiveCAA93594.1ENA Protein Mapping
UniParcUPI000012F6C0UniProt Archive

Literature for met16

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014