SPAC1527.03


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC1527.03 Feature Typeprotein coding
Synonyms Name Description
ProductRNA-binding protein (predicted) Product Size475aa, 53.23 kDa
Genomic Location Chromosome I, 4384450-4386950 (2501nt); CDS:4384659-4386086 (1428nt)

Ensembl Gene Location
  1. mok11
  2. sft2
  3. SPAC1527.03
  4. sec72
  5. SPAC30.02c
GO Molecular Function
Term NameCount
RNA binding644
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translation470
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4206
Annotation ExtensionEvidenceWith/FromReference
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
polysome5
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationnot recorded (wild type)1427
viable vegetative cell populationSPAC1527.03ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphology3092
penetrance FYPO_EXT:0000001SPAC1527.03ΔNull
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
143844504386950

UTRs

Region Coordinates Reference
three_prime_UTR4386087..4386950PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF05383 Pfam IPR006630 RNA-binding protein Lupus La 330 386 2
SM00715 SMART IPR006630 RNA-binding protein Lupus La 323 406 2
PS50961 Prosite Profiles IPR006630 RNA-binding protein Lupus La 319 408 2
PTHR22792:SF2 HMMPANTHER 50 419 1
PTHR22792 HMMPANTHER 50 419 2
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 326 409 46
SSF46785 SuperFamily 320 414 49

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.07 Da
Charge 17.00
Isoelectric point 9.52
Molecular weight 53.23 kDa
Number of residues 475
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS2
present during mitotic M phaseS65
S2, S116, S410
present during mitotic M phaseS410
present during mitotic M phaseS7
Annotation ExtensionEvidenceResidueReference
experimental evidence S2 PMID:24763107
present during mitotic M phase experimental evidence S2 PMID:21712547
present during mitotic M phase experimental evidence S7 PMID:21712547
present during mitotic M phase experimental evidence S65 PMID:21712547
experimental evidence S116 PMID:24763107
experimental evidence S410 PMID:24763107
present during mitotic M phase experimental evidence S410 PMID:21712547
O-phospho-L-threonineT51 693
present during mitotic M phaseT51
Annotation ExtensionEvidenceResidueReference
experimental evidence T51 PMID:24763107
present during mitotic M phase experimental evidence T51 PMID:21712547
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9063during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
8933during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
8027during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
7245.64during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
5987.73during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.48during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
mtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
pir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
red5human ZC3H3 homolog Affinity Capture-MSPMID:24713849
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
red1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
sre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
atg1autophagy and CVT pathway serine/threonine protein kinase Atg1 Positive GeneticPMID:22681890
SPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
tas3RITS complex subunit 3 Positive GeneticPMID:22681890
par1protein phosphatase regulatory subunit Par1 Positive GeneticPMID:22681890
usp102U1 snRNP-associated protein Usp102 Positive GeneticPMID:22681890
epe1Jmjc domain chromatin associated protein Epe1 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC1527.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1527.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1527.03 BioGRID Interaction Datasets
Expression Viewer SPAC1527.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1527.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1527.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1527.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1527.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1527.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1527.03 Cell Cycle Data
GEO SPAC1527.03 GEO profiles
PInt SPAC1527.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1527.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1527.03 Fission yeast phenotypic data & analysis
SPD / RIKEN21/21H12Orfeome Localization Data
UniProtKB/SwissProtQ9P6K0Uncharacterized HTH La-type RNA-binding protein C1527.03
ModBaseQ9P6K0Database of comparative protein structure models
STRINGQ9P6K0Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594554RNA-binding protein (predicted)
RefSeq mRNANM_001019983972h- RNA-binding protein (predicted) (SPAC1527.03), mRNA
European Nucleotide ArchiveCAB90798.1ENA Protein Mapping
UniParcUPI0000069C08UniProt Archive

Literature for SPAC1527.03

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015