SPAC1527.03


Gene Standard NameUnassigned Characterisation Statusbiological role inferred
Systematic IDSPAC1527.03 Feature Typeprotein coding
Synonyms Name Description
ProductRNA-binding protein (predicted) Product Size475aa, 53.23 kDa
Genomic Location Chromosome I, 4384450-4386950 (2501nt); CDS:4384659-4386086 (1428nt)

Ensembl Gene Location
  1. mok11
  2. sft2
  3. SPAC1527.03
  4. sec72
  5. SPAC30.02c
GO Molecular Function
Term NameCount
RNA binding588
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translation469
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
polysome5
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationSPAC1527.03+ (wild type)1455
viable vegetative cell populationSPAC1527.03Δ3850

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologySPAC1527.03Δ3102
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4384450..4386950
mRNA4384450..4386950
5' UTR4384450..4384658AU009887
CDS4384659..4386086
3' UTR4386087..4386950PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF05383 Pfam IPR006630 RNA-binding protein Lupus La 331 384 2
SM00715 SMART IPR006630 RNA-binding protein Lupus La 323 406 2
PS50961 Prosite Profiles IPR006630 RNA-binding protein Lupus La 319 408 2
PTHR22792 HMMPANTHER 152 160 2
PTHR22792 HMMPANTHER 312 419 2
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 326 409 46
SSF46785 SuperFamily IPR011991 Winged helix-turn-helix DNA-binding domain 320 414 49

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.07 Da
Charge 17.00
Codon Adaptation Index 0.44
Isoelectric point 9.52
Molecular weight 53.23 kDa
Number of residues 475
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS410
present during mitotic M phaseS2
present during mitotic M phaseS7
present during mitotic M phaseS65
S2, S46, S116, S130, S240, S243, S410
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S410 PMID:21712547
present during mitotic M phase experimental evidence S2 PMID:21712547
present during mitotic M phase experimental evidence S7 PMID:21712547
present during mitotic M phase experimental evidence S65 PMID:21712547
experimental evidence S410 PMID:24763107
IDA S243 PMID:25720772
IDA S240 PMID:25720772
IDA S410 PMID:25720772
experimental evidence S116 PMID:24763107
IDA S130 PMID:25720772
experimental evidence S2 PMID:24763107
IDA S46 PMID:25720772
O-phospho-L-threonine 1085
present during mitotic M phaseT51
T48, T51, T467, T474
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T51 PMID:21712547
IDA T474 PMID:25720772
IDA T467 PMID:25720772
IDA T48 PMID:25720772
experimental evidence T51 PMID:24763107
phosphorylated residue 2511
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
O-phosphorylated residueS205,S208, T48,T51 2457
Annotation ExtensionEvidenceResidueReference
IDA T48,T51 PMID:25720772
IDA S205,S208 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for SPAC1527.03

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9063during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8933during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8027during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
7245.64during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
5987.73during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.48during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC1527.03 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bymtl1TRAMP/MTREC complex subunit, ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
affinity captured byred5human ZC3H3 homolog Affinity Capture-MSPMID:24713849
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC1527.03 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withusp102U1 snRNP-associated protein Usp102 Positive GeneticPMID:22681890
positive genetic interaction withpar1protein phosphatase PP2A regulatory subunit B-56 Par1 Positive GeneticPMID:22681890
positive genetic interaction withatg1autophagy serine/threonine protein kinase Atg1 Positive GeneticPMID:22681890
positive genetic interaction withtas3RITS complex subunit 3 Positive GeneticPMID:22681890
positive genetic interaction withurk1uridine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withepe1Jmjc domain chromatin associated protein Epe1 Positive GeneticPMID:22681890
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
External References
Database Identifier Description
NBRP SPAC1527.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1527.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1527.03 BioGRID Interaction Datasets
Expression Viewer SPAC1527.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1527.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1527.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1527.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1527.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1527.03 Transcriptome Viewer (Bähler Lab)
GEO SPAC1527.03 GEO profiles
PInt SPAC1527.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1527.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1527.03 Fission yeast phenotypic data & analysis
Cyclebase SPAC1527.03.1 Cell Cycle Data
SPD / RIKEN21/21H12Orfeome Localization Data
UniProtKB/SwissProtQ9P6K0Uncharacterized HTH La-type RNA-binding protein C1527.03
ModBaseQ9P6K0Database of comparative protein structure models
STRINGQ9P6K0Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594554RNA-binding protein (predicted)
RefSeq mRNANM_001019983972h- RNA-binding protein (predicted) (SPAC1527.03), mRNA
European Nucleotide ArchiveCAB90798.1ENA Protein Mapping
UniParcUPI0000069C08UniProt Archive

Literature for SPAC1527.03

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016