SPAC1527.03


Gene Standard NameUnassigned Characterisation Statusbiological_role_inferred
Systematic IDSPAC1527.03 Feature Typeprotein coding
Synonyms Name Description
ProductRNA-binding protein (predicted) Product Size475aa, 53.23 kDa
Genomic Location Chromosome I, 4384450-4386950 (2501nt); CDS:4384659-4386086 (1428nt)

Ensembl Gene Location
  1. mok11
  2. sft2
  3. SPAC1527.03
  4. sec72
  5. SPAC30.02c
GO Molecular Function
Term NameCount
RNA binding569
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translation462
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4208
Annotation ExtensionEvidenceWith/FromReference
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
polysome5
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationSPAC1527.03+ (wild type)Overexpression1428
viable vegetative cell populationSPAC1527.03ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologySPAC1527.03ΔNull3092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4384450..4386950
mRNA4384450..4386950
5' UTR4384450..4384658AU009887
CDS4384659..4386086
3' UTR4386087..4386950PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF05383 Pfam IPR006630 RNA-binding protein Lupus La 330 386 2
SM00715 SMART IPR006630 RNA-binding protein Lupus La 323 406 2
PS50961 Prosite Profiles IPR006630 RNA-binding protein Lupus La 319 408 2
PTHR22792 HMMPANTHER 50 419 2
PTHR22792:SF2 HMMPANTHER 50 419 1
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 326 409 46
SSF46785 SuperFamily 320 414 49

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.07 Da
Charge 17.00
Isoelectric point 9.52
Molecular weight 53.23 kDa
Number of residues 475
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS65
present during mitotic M phaseS7
S2, S116, S410
present during mitotic M phaseS410
present during mitotic M phaseS2
Annotation ExtensionEvidenceResidueReference
experimental evidence S2 PMID:24763107
present during mitotic M phase experimental evidence S2 PMID:21712547
present during mitotic M phase experimental evidence S7 PMID:21712547
present during mitotic M phase experimental evidence S65 PMID:21712547
experimental evidence S116 PMID:24763107
experimental evidence S410 PMID:24763107
present during mitotic M phase experimental evidence S410 PMID:21712547
O-phospho-L-threonineT51 698
present during mitotic M phaseT51
Annotation ExtensionEvidenceResidueReference
experimental evidence T51 PMID:24763107
present during mitotic M phase experimental evidence T51 PMID:21712547
phosphorylated residue 1926
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
9063during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
8933during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
7245.64during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
8027during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5987.73during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.48during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
affinity captured bymtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
affinity captured bypir2zf-C2H2 type zinc finger protein, implicated in RNAi (predicted) Affinity Capture-MSPMID:24713849
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
affinity captured byred5human ZC3H3 homolog Affinity Capture-MSPMID:24713849
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withatg1autophagy and CVT pathway serine/threonine protein kinase Atg1 Positive GeneticPMID:22681890
positive genetic interaction withepe1Jmjc domain chromatin associated protein Epe1 Positive GeneticPMID:22681890
positive genetic interaction withpar1protein phosphatase regulatory subunit Par1 Positive GeneticPMID:22681890
positive genetic interaction withSPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:21504829
negative genetic interaction withsre2membrane-tethered transcription factor Sre2 Negative GeneticPMID:22681890
positive genetic interaction withtas3RITS complex subunit 3 Positive GeneticPMID:22681890
positive genetic interaction withusp102U1 snRNP-associated protein Usp102 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC1527.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1527.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1527.03 BioGRID Interaction Datasets
Expression Viewer SPAC1527.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1527.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1527.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1527.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1527.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1527.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1527.03 Cell Cycle Data
GEO SPAC1527.03 GEO profiles
PInt SPAC1527.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1527.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1527.03 Fission yeast phenotypic data & analysis
SPD / RIKEN21/21H12Orfeome Localization Data
UniProtKB/SwissProtQ9P6K0Uncharacterized HTH La-type RNA-binding protein C1527.03
ModBaseQ9P6K0Database of comparative protein structure models
STRINGQ9P6K0Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594554RNA-binding protein (predicted)
RefSeq mRNANM_001019983972h- RNA-binding protein (predicted) (SPAC1527.03), mRNA
European Nucleotide ArchiveCAB90798.1ENA Protein Mapping
UniParcUPI0000069C08UniProt Archive

Literature for SPAC1527.03

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015