ste4 (SPAC1565.04c)


Gene Standard Nameste4 Characterisation Statuspublished
Systematic IDSPAC1565.04c Feature Typeprotein coding
Synonyms Name DescriptionSTErile
Productadaptor protein Ste4 Product Size264aa, 30.74 kDa
Genomic Location Chromosome I, 1296634-1295316 (1319nt); CDS:1296510-1295558 (953nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding863
Annotation ExtensionEvidenceWith/FromReference
protein kinase regulator activity34
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytoplasm4211
Annotation ExtensionEvidenceWith/FromReference
cytosol2307
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencyste4Δ252
Δ C-terminus (161-264)
decreased sporulation frequencyste4+95
pst1-spe1Δ (region between restriction sites deleted)
sph1-hindIIIΔ (region between restriction sites deleted)
spe1-sph1Δ (region between restriction sites deleted)
spe1-frameshift (frameshift mutation at spe1 site)
sph1-frameshift (frameshift mutation at sph1 site)
xba1-xba1Δ (region between restriction sites deleted)
normal vegetative cell population growthste4Δ782
viable vegetative cell populationste4Δ3819

Cell Phenotype

Term NameGenotypesCount
abnormal response to pheromoneste4-JMl0425
abolished protein binding104
affecting ste4Δ N-terminus (1-160)
affecting ste4 and byr2Δ N-terminus (1-160)
abolished sporulationste4-/ste4- (homozygous diploid)54
decreased concentration of P-factor in growth mediumste4-JMl049
decreased protein level during cellular response to nitrogen starvation20
affecting mat2-Pisterile ste4
sterileste4-JM10457
ste4Δ
viable vegetative cell with normal cell morphologyste4Δ3097

Multi-allele phenotypes


Cell Phenotype

Term NameGenotypes
meiosis and sporulation in haploidpat1-114, ste4-JM104
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in byr2 MAP kinase kinase kinase Byr2
FYPO affected by mutation in ste11 transcription factor Ste11
FYPO affected by mutation in ste4 adaptor protein Ste4
GO regulated by ste11 transcription factor Ste11
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1296634..1296414, 1296255..1295316
Intron1296413..1296256
mRNA1296634..1295316
5' UTR1296634..1296511PMID:21511999
CDS1296510..1296414, 1296255..1295558
3' UTR1295557..1295316PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07647 Pfam IPR001660 Sterile alpha motif domain 8 71 4
PF00788 Pfam IPR000159 Ras-associating (RA) domain 179 263 1
SM00454 SMART IPR001660 Sterile alpha motif domain 8 73 4
SM00314 SMART IPR000159 Ras-associating (RA) domain 176 264 2
PS50200 Prosite Profiles IPR000159 Ras-associating (RA) domain 176 264 2
PS50105 Prosite Profiles IPR001660 Sterile alpha motif domain 11 73 4
1.10.150.50 Gene3D IPR013761 Sterile alpha motif/pointed domain 10 72 4
SSF47769 SuperFamily IPR013761 Sterile alpha motif/pointed domain 8 77 4
Coil ncoils Predicted coiled-coil protein (DUF2205) 64 135 1048

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.42 Da
Charge -7.00
Isoelectric point 5.00
Molecular weight 30.74 kDa
Number of residues 264
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS105 1674
Annotation ExtensionEvidenceResidueReference
experimental evidence S105 PMID:24763107
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0006995Northern assay evidencePMID:1766866
present during GO:0072690Northern assay evidencePMID:1766866

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
342.77during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.57during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.26during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi only620
conserved in fungi4604
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPAC1565.04c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withbyr2MAP kinase kinase kinase Byr2 Two-hybridPMID:9315645
forms complex withbyr2MAP kinase kinase kinase Byr2 Reconstituted ComplexPMID:12171939
binds activation domain construct withbyr2MAP kinase kinase kinase Byr2 Two-hybridPMID:23695164
binds activation domain construct withste4adaptor protein Ste4 Two-hybridPMID:8816472
Genetic Interactions

Source: BioGRID

Load gene that interact geneitically with SPAC1565.04c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withsgf73SAGA complex subunit Sgf73 Negative GeneticPMID:25076038
rescued byras1GTPase Ras1 Phenotypic SuppressionPMID:8816472
rescued bybyr2MAP kinase kinase kinase Byr2 Phenotypic SuppressionPMID:8816472
rescued bybyr1MAP kinase kinase Byr1 Phenotypic SuppressionPMID:8816472
synthetic lethal withfan1Fanconi-associated nuclease Fan1 Synthetic LethalityPMID:24192486
rescuesgpa1G-protein alpha subunit Phenotypic SuppressionPMID:8816472
rescuesras1GTPase Ras1 Phenotypic SuppressionPMID:8816472
External References
Database Identifier Description
NBRP SPAC1565.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1565.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1565.04c BioGRID Interaction Datasets
Expression Viewer SPAC1565.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1565.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1565.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1565.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1565.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1565.04c Transcriptome Viewer (Bähler Lab)
GEO SPAC1565.04c GEO profiles
PInt SPAC1565.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1565.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1565.04c Fission yeast phenotypic data & analysis
Cyclebase SPAC1565.04c.1 Cell Cycle Data
SPD / RIKEN13/13D07Orfeome Localization Data
UniProtKB/SwissProtP36622Sexual differentiation protein ste4
ModBaseP36622Database of comparative protein structure models
STRINGP36622Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593283adaptor protein Ste4
RefSeq mRNANM_001018713972h- adaptor protein Ste4 (ste4), mRNA
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveX61924ENA EMBL mapping
European Nucleotide ArchiveCAA43926ENA Protein Mapping
European Nucleotide ArchiveCAA43926.1ENA Protein Mapping
European Nucleotide ArchiveCAB99271ENA Protein Mapping
European Nucleotide ArchiveCAB99271.1ENA Protein Mapping
UniParcUPI00001360DBUniProt Archive

Literature for ste4

Search: Europe PMC or PubMed

Release Version: PomBase:29_56 - 12 Nov 2015