ste4 (SPAC1565.04c)


Gene Standard Nameste4 Characterisation Statuspublished
Systematic IDSPAC1565.04c Feature Typeprotein coding
Synonyms Name DescriptionSTErile
Productadaptor protein Ste4 Product Size264aa, 30.74 kDa
Genomic Location Chromosome I, 1296634-1295316 (1319nt); CDS:1296510-1295558 (953nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005515protein binding887
requires Pfam:PF07647IPIbyr2PMID:12171939
GO:0019887protein kinase regulator activity32
regulates byr2IPIbyr2PMID:12171939
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006995cellular response to nitrogen starvationIGIbyr2PMID:217193993
GO:0071444cellular response to pheromoneISOSGD:S000000537GO_REF:000002428
GO:0000750pheromone-dependent signal transduction involved in conjugation with cellular fusionIMPPMID:797589412
ISOSGD:S000000537GO_REF:0000024
GO:0045859regulation of protein kinase activityIPIbyr2PMID:1217193952
GO:0009966regulation of signal transductionICGO:0019887GO_REF:0000001136
GO:0032005signal transduction involved in conjugation with cellular fusionIGIbyr2PMID:1217193919
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISOSGD:S000000537GO_REF:00000244198
GO:0005829cytosolIDAPMID:168233722317
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyste4ΔNullPECO:0000005, PECO:0000137PMID:236978063755
Microscopyste4ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000302abnormal response to pheromoneMicroscopyste4-JMl04Not specifiedPMID:193412120
FYPO:0000822decreased concentration of P-factor in growth mediumMicroscopyste4-JMl04Not specifiedPMID:19341219
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidenceste4ΔNullPECO:0000015PMID:23950735222
FYPO:0001283decreased protein level during cellular response to nitrogen starvationReporter gene assaysterile ste4Not specifiedPMID:797589412
affecting matPi
FYPO:0000584decreased sporulationCell growth assaypst1-spe1Δ (region between restriction sites deleted)Not specifiedPMID:176686635
Cell growth assayste4+ (wild type)OverexpressionPMID:1766866
Cell growth assayxba1-xba1Δ (region between restriction sites deleted)OverexpressionPMID:1766866
Cell growth assayxba1-xba1Δ (region between restriction sites deleted)Not specifiedPMID:1766866
Cell growth assaysph1-frameshift (frameshift mutation at sph1 site)Not specifiedPMID:1766866
Cell growth assayspe1-sph1Δ (region between restriction sites deleted)Not specifiedPMID:1766866
Cell growth assaysph1-hindIIIΔ (region between restriction sites deleted)Not specifiedPMID:1766866
Cell growth assayspe1-frameshift (frameshift mutation at spe1 site)Not specifiedPMID:1766866
FYPO:0000280sterileCell growth assayste4ΔNullPMID:176686639
Otherste4-JM104Not specifiedPMID:3185514
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopyste4ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Target Of
RelationshipGeneProductReference
regulated by ste11 transcription factor Ste11 PMID:17032641
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
112966341296414
212962551295316

UTRs

Region Start End Reference
three_prime_UTR12955571295316PMID:21511999
five_prime_UTR12966341296511PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07647 Pfam IPR011510 Sterile alpha motif, type 2 8 71 4
PF00788 Pfam IPR000159 Ras-association 184 263 2
SM00454 SMART IPR001660 Sterile alpha motif domain 8 73 5
SM00314 SMART IPR000159 Ras-association 176 264 2
PS50200 Prosite Profiles IPR000159 Ras-association 176 264 2
PS50105 Prosite Profiles IPR001660 Sterile alpha motif domain 11 73 4
PTHR12844 HMMPANTHER 1 140 1
G3DSA:1.10.150.50 Gene3D IPR013761 10 72 4
SSF47769 SuperFamily IPR013761 8 77 4
Coil ncoils Rabaptin coiled-coil domain 64 136 975

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.42 Da
Charge -7.00
Isoelectric point 5.00
Molecular weight 30.74 kDa
Number of residues 264
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreasedNorthern assay evidencePMID:1766866
during GO:0006995
presentNorthern assay evidencePMID:1766866
during GO:0072690

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
342.77during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.26during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
0.57during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi only603
conserved in fungi4564
conserved in eukaryotes4482
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
fan1Fanconi-associated nuclease Fan1 Synthetic LethalityPMID:24192486
ras1GTPase Ras1 Phenotypic SuppressionPMID:8816472
byr1MAP kinase kinase Byr1 Phenotypic SuppressionPMID:8816472
byr2MAP kinase kinase kinase Byr2 Phenotypic SuppressionPMID:9315645
Phenotypic SuppressionPMID:8816472
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
byr2MAP kinase kinase kinase Byr2 Two-hybridPMID:9315645
Reconstituted ComplexPMID:12171939
Reconstituted ComplexPMID:15364564
Two-hybridPMID:23695164
Two-hybridPMID:8816472
Reconstituted Complex
External References
Database Identifier Description
NBRP SPAC1565.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1565.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1565.04c BioGRID Interaction Datasets
Expression Viewer SPAC1565.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1565.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1565.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1565.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1565.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1565.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1565.04c Cell Cycle Data
GEO SPAC1565.04c GEO profiles
PInt SPAC1565.04c Protein-Protein Interaction Predictor (Bähler Lab)
SPD / RIKEN13/13D07Orfeome Localization Data
WikiGene2541677adaptor protein Ste4
EntrezGene2541677adaptor protein Ste4
UniProtKB/SwissProtP36622Sexual differentiation protein ste4
ModBaseP36622Database of comparative protein structure models
StringP36622Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593283adaptor protein Ste4
RefSeq mRNANM_001018713972h- adaptor protein Ste4 (ste4), mRNA
European Nucleotide ArchiveCAA43926ENA Protein Mapping
European Nucleotide ArchiveCAB99271ENA Protein Mapping
UniParcUPI00001360DBUniProt Archive

Literature for ste4

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014