ste4 (SPAC1565.04c)


Gene Standard Nameste4 Characterisation Statuspublished
Systematic IDSPAC1565.04c Feature Typeprotein coding
Synonyms Name DescriptionSTErile
Productadaptor protein Ste4 Product Size264aa, 30.74 kDa
Genomic Location Chromosome I, 1296634-1295316 (1319nt); CDS:1296510-1295558 (953nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding831
Annotation ExtensionEvidenceWith/FromReference
protein homodimerization activity3
Annotation ExtensionEvidenceWith/FromReference
protein kinase regulator activity32
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytoplasm4205
Annotation ExtensionEvidenceWith/FromReference
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001357normal vegetative cell population growthCell growth assayste4ΔNullPMID:8816472641
FYPO:0002060viable vegetative cell populationMicroscopyste4ΔNullPECO:0000005, PECO:0000137PMID:236978063759
Microscopyste4ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000302abnormal response to pheromoneMicroscopyste4-JMl04Not specifiedPMID:193412119
FYPO:0000705abolished protein binding58
affecting ste4 and byr2Reporter gene assayΔ N-terminus (1-160)Not specifiedPMID:8816472
FYPO:0000583abolished sporulationCell growth assayste4-/ste4- (homozygous diploid)Not specifiedPMID:881647251
FYPO:0000822decreased concentration of P-factor in growth mediumMicroscopyste4-JMl04Not specifiedPMID:19341219
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidenceste4ΔNullPECO:0000015PMID:23950735240
Cell growth assayΔ C-terminus (161-264)Not specifiedPMID:8816472
FYPO:0001283decreased protein level during cellular response to nitrogen starvation10
affecting mat2-PiReporter gene assaysterile ste4Not specifiedPMID:7975894
FYPO:0000584decreased sporulationCell growth assayspe1-sph1Δ (region between restriction sites deleted)Not specifiedPMID:176686661
Cell growth assayxba1-xba1Δ (region between restriction sites deleted)Not specifiedPMID:1766866
Cell growth assayste4+ (wild type)OverexpressionPMID:1766866
Cell growth assaysph1-frameshift (frameshift mutation at sph1 site)Not specifiedPMID:1766866
Cell growth assaypst1-spe1Δ (region between restriction sites deleted)Not specifiedPMID:1766866
Cell growth assayxba1-xba1Δ (region between restriction sites deleted)OverexpressionPMID:1766866
Cell growth assayspe1-frameshift (frameshift mutation at spe1 site)Not specifiedPMID:1766866
Cell growth assaysph1-hindIIIΔ (region between restriction sites deleted)Not specifiedPMID:1766866
FYPO:0000280sterileOtherste4-JM104Not specifiedPMID:318551450
Cell growth assayste4ΔNullPMID:1766866
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyste4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopyste4ΔNullPMID:8816472
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in byr2 MAP kinase kinase kinase Byr2 PMID:8816472
FYPO affected by mutation in ste11 transcription factor Ste11 PMID:1766866
FYPO affected by mutation in ste4 adaptor protein Ste4 PMID:8816472
GO regulated by ste11 transcription factor Ste11 PMID:17032641
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
112966341296414
212962551295316

UTRs

Region Coordinates Reference
five_prime_UTR1296634..1296511PMID:21511999
three_prime_UTR1295557..1295316PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07647 Pfam IPR011510 Sterile alpha motif, type 2 8 71 4
PF00788 Pfam IPR000159 Ras-association 184 263 2
SM00454 SMART IPR001660 Sterile alpha motif domain 8 73 4
SM00314 SMART IPR000159 Ras-association 176 264 2
PS50200 Prosite Profiles IPR000159 Ras-association 176 264 2
PS50105 Prosite Profiles IPR001660 Sterile alpha motif domain 11 73 4
PTHR12844 HMMPANTHER 1 140 1
1.10.150.50 Gene3D IPR013761 Sterile alpha motif/pointed domain 10 72 4
SSF47769 SuperFamily IPR013761 Sterile alpha motif/pointed domain 8 77 4
Coil ncoils Rabaptin coiled-coil domain 64 136 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.42 Da
Charge -7.00
Isoelectric point 5.00
Molecular weight 30.74 kDa
Number of residues 264
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS105PMID:247631071670
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelpresent during GO:0072690Northern assay evidencePMID:1766866
increased during GO:0006995Northern assay evidencePMID:1766866

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
342.77during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.57during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.26during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only623
conserved in fungi4600
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
ste4adaptor protein Ste4 Two-hybridPMID:8816472
byr2MAP kinase kinase kinase Byr2 Two-hybridPMID:9315645
Reconstituted ComplexPMID:12171939
Reconstituted ComplexPMID:15364564
Two-hybridPMID:23695164
Two-hybridPMID:8816472
Reconstituted Complex
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
byr1MAP kinase kinase Byr1 Phenotypic SuppressionPMID:8816472
ras1GTPase Ras1 Phenotypic SuppressionPMID:8816472
sgf73SAGA complex subunit Sgf73 Negative GeneticPMID:25076038
gpa1G-protein alpha subunit Phenotypic SuppressionPMID:8816472
fan1Fanconi-associated nuclease Fan1 Synthetic LethalityPMID:24192486
byr2MAP kinase kinase kinase Byr2 Phenotypic SuppressionPMID:9315645
Dosage RescuePMID:8816472
Phenotypic Suppression
External References
Database Identifier Description
NBRP SPAC1565.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1565.04c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1565.04c BioGRID Interaction Datasets
Expression Viewer SPAC1565.04c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1565.04c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1565.04c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1565.04c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1565.04c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1565.04c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1565.04c Cell Cycle Data
GEO SPAC1565.04c GEO profiles
PInt SPAC1565.04c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1565.04c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1565.04c Fission yeast phenotypic data & analysis
SPD / RIKEN13/13D07Orfeome Localization Data
UniProtKB/SwissProtP36622Sexual differentiation protein ste4
ModBaseP36622Database of comparative protein structure models
STRINGP36622Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593283adaptor protein Ste4
RefSeq mRNANM_001018713972h- adaptor protein Ste4 (ste4), mRNA
European Nucleotide ArchiveCAA43926.1ENA Protein Mapping
European Nucleotide ArchiveCAB99271.1ENA Protein Mapping
UniParcUPI00001360DBUniProt Archive

Literature for ste4

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014