ste4 (SPAC1565.04c)

Gene Standard Nameste4 ChromosomeI
Systematic IDSPAC1565.04c Gene Start1296634
Synonyms Gene End1295316
Productadaptor protein Ste4 Gene Length1319
Feature Typeprotein coding CDS Start1296510
Name DescriptionSTErile CDS End1295558
Characterisation Statuspublished CDS Length953

Ensembl Gene Location
  1. SPAPB17E12.14c
  2. SPNCRNA.732
  3. SPAC1565.01
  4. SPAC1565.02c
  5. SPNCRNA.733
  6. SPAC1565.03
  7. ste4
  8. SPAC1565.05
  9. spg1
  10. SPAC1565.07c
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000280sterileCell growth assayste4delta (deletion)deletionNot specifiedPMID:1766866
Otherste4-JM104 (unknown)unknownNot specifiedPMID:3185514
FYPO:0000302abnormal response to pheromoneMicroscopyste4-JMl04 (unknown)unknownNot specifiedPMID:1934121
FYPO:0000584decreased sporulationCell growth assayxba1-xba1delta (region between restriction sites deleted)otherNot specifiedPMID:1766866
Cell growth assayspe1-sph1delta (region between restriction sites deleted)otherNot specifiedPMID:1766866
Cell growth assayxba1-xba1delta (region between restriction sites deleted)otherOverexpressionPMID:1766866
Cell growth assaypst1-spe1delta (region between restriction sites deleted)otherNot specifiedPMID:1766866
Cell growth assayste4+ (wild type)wild_typeOverexpressionPMID:1766866
Cell growth assaysph1-frameshift (frameshift mutation at sph1 site)otherNot specifiedPMID:1766866
Cell growth assayspe1-frameshift (frameshift mutation at spe1 site)otherNot specifiedPMID:1766866
Cell growth assaysph1-hindIIIdelta (region between restriction sites deleted)otherNot specifiedPMID:1766866
FYPO:0000822decreased concentration of P-factor in growth mediumMicroscopyste4-JMl04 (unknown)unknownNot specifiedPMID:1934121
FYPO:0001283decreased protein level during cellular response to nitrogen starvationReporter gene assaysterile ste4 (unknown)unknownNot specifiedPMID:7975894
assayed_using matPi
FYPO:0002060viable vegetative cell populationMicroscopyste4delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0005515protein binding
requires_sequence_feature Pfam:PF07647IPIbyr2PMID:12171939
GO:0019887protein kinase regulator activity
has_direct_input byr2IPIbyr2PMID:12171939
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000750pheromone-dependent signal transduction involved in conjugation with cellular fusionIMPPMID:7975894
ISOSGD:S000000537GO_REF:0000024
GO:0006995cellular response to nitrogen starvationIGIbyr2PMID:2171939
GO:0009966regulation of signal transductionICGO:0019887GO_REF:0000001
GO:0032005signal transduction involved in conjugation with cellular fusionIGIbyr2PMID:12171939
GO:0071444cellular response to pheromoneISOSGD:S000000537GO_REF:0000024
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0005737cytoplasmISOSGD:S000000537GO_REF:0000024
GO:0005829cytosolIDAPMID:16823372
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
112966341296414
212962551295316

References

Region Start End Reference
three_prime_UTR12955571295316PMID:21511999
three_prime_UTR12955571295316PMID:21511999
five_prime_UTR12966341296511PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00788 IPR000159 Pfam Ras-association 184 263 2
PF07647 IPR011510 Pfam Sterile alpha motif, type 2 8 71 4
SM00454 IPR001660 SMART Sterile alpha motif domain 8 73 5
SM00314 IPR000159 SMART Ras-association 176 264 2
PS50200 IPR000159 Prosite Profiles Ras-association 176 264 2
PS50105 IPR001660 Prosite Profiles Sterile alpha motif domain 11 73 4
G3DSA:1.10.150.50 IPR013761 Gene3D 10 72 4
SSF47769 IPR013761 SuperFamily 8 77 4
Coil ncoils Rabaptin coiled-coil domain 64 136 974

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.42 Da
Charge -7.00
Isoelectric point 5.00
Molecular weight 30.74 kDa
Number of residues 264
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide342.77PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127		population_widePMID:23101633
RNA levelNorthern assay evidencecellular response to nitrogen starvationincreasedPMID:1766866
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.26PMID:23101633
Northern assay evidencesingle-celled organism vegetative growth phasepresentPMID:1766866
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide0.57PMID:23101633
Species Distribution
DescriptionQualifierReference
predominantly single copy (one to one)
conserved in fungi only
conserved in fungi
conserved in eukaryotes
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
byr2MAP kinase kinase kinase Byr2 Phenotypic SuppressionPMID:9315645
Phenotypic SuppressionPMID:8816472
byr1MAP kinase kinase Byr1 Phenotypic SuppressionPMID:8816472
ras1GTPase Ras1 Phenotypic SuppressionPMID:8816472
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
byr2MAP kinase kinase kinase Byr2 Two-hybridPMID:9315645
Two-hybridPMID:8816472
Reconstituted Complex
Reconstituted ComplexPMID:15364564
Reconstituted ComplexPMID:12171939
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External References
Database Identifier Description
NBRP SPAC1565.04c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1565.04c Retrieval of eukaryotic orthologs
BioGrid SPAC1565.04c BioGRID Interaction Datasets
Bähler Lab SPAC1565.04c Cell Cycle Expression Profile
Bähler Lab SPAC1565.04c Meiosis/Sporulation Expression Profies
Bähler Lab SPAC1565.04c Pheromone response/mating expression profiles
Bähler Lab SPAC1565.04c Environmental stress expression profiles
Bähler Lab SPAC1565.04c Bähler Lab Transcriptome Viewer
Cyclebase SPAC1565.04c Cell Cycle Data
PInt SPAC1565.04c Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC1565.04c Entrez Gene
SPD / RIKEN13/13D07Orfeome Localization Data
WikiGene2541677adaptor protein Ste4
Uniprot_genenameSTE4
EntrezGene2541677adaptor protein Ste4
UniProtKB/SwissProtP36622Sexual differentiation protein ste4
ModBaseP36622Database of comparative protein structure models
Pfam Protein DomainsP36622Pfam Domain Arrangement
RefSeq PeptideNP_593283adaptor protein Ste4
RefSeq mRNANM_001018713972h- adaptor protein Ste4 (ste4), mRNA
European Nucleotide ArchiveCAA43926ENA Protein Mapping
European Nucleotide ArchiveCAB99271ENA Protein Mapping
UniParcUPI00001360DBUniProt Archive

Literature for ste4
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013