spg1 (SPAC1565.06c)

Gene Standard Namespg1 ChromosomeI
Systematic IDSPAC1565.06c Gene Start1301178
Synonymssid3 Gene End1299707
ProductGTPase Spg1 Gene Length1472
Feature Typeprotein coding CDS Start1301018
Name DescriptionSeptum Initiation Defective,
septum promoting GTPase		 CDS End1300144
Characterisation Statuspublished CDS Length875

Ensembl Gene Location
  1. SPAC1565.01
  2. SPAC1565.02c
  3. SPNCRNA.733
  4. SPAC1565.03
  5. ste4
  6. SPAC1565.05
  7. spg1
  8. SPAC1565.07c
  9. SPNCRNA.169
  10. cdc48
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000061multinucleateMicroscopyT24N (T24N)amino_acid_mutationNot specifiedPECO:0000005PMID:9203579
FYPO:0000082sensitive to high temperatureCell growth assayspg1-B8 (unknown)unknownNot specifiedPMID:9203579
FYPO:0000272abolished septum formationno_name (unrecorded)unrecordedPMID:9649519
FYPO:0000444cell cycle arrest with replicated DNAFlow cytometry dataspg1+ (wild type)wild_typeOverexpressionPMID:9203579
FYPO:0000650increased septation indexMicroscopyQ69L (Q69L)amino_acid_mutationNot specifiedPECO:0000005PMID:9203579
has_expressivity FYPO_EXT:0000001
Microscopyspg1+ (wild type)wild_typeOverexpressionPECO:0000126, PECO:0000005, PECO:0000103PMID:9203579
has_expressivity FYPO_EXT:0000003MicroscopyT24N (T24N)amino_acid_mutationNot specifiedPECO:0000005PMID:9203579
has_expressivity FYPO_EXT:0000001MicroscopyD125A (D125A)amino_acid_mutationOverexpressionPECO:0000005PMID:9203579
FYPO:0001128decreased septation indexMicroscopyT42A (T42A)amino_acid_mutationNot specifiedPECO:0000005PMID:9203579
FYPO:0001161decreased aerobic cell population growth on glucose carbon sourceCell growth assayspg1-B8 (unknown)unknownNot specifiedPECO:0000005, PECO:0000137, PECO:0000102PMID:9203579
Cell growth assayspg1-B8 (unknown)unknownNot specifiedPECO:0000102, PECO:0000137, PECO:0000006PMID:9203579
FYPO:0001234slow vegetative cell population growthCell growth assayspg1+ (wild type)wild_typeOverexpressionPECO:0000126, PECO:0000103, PECO:0000005PMID:9203579
FYPO:0001368normal actomyosin contractile ring assemblyMicroscopyspg1delta (deletion)deletionNot specifiedPECO:0000103, PECO:0000126, PECO:0000005PMID:9203579
Microscopyspg1-B8 (unknown)unknownNot specifiedPECO:0000103, PECO:0000126, PECO:0000005PMID:9203579
FYPO:0001645decreased protein bindingReporter gene assayT42A (T42A)amino_acid_mutationNot specifiedPMID:9203579
assayed_using cdc7
assayed_using spg1
FYPO:0001905normal mitotic spindle microtubulesMicroscopyspg1delta (deletion)deletionNot specifiedPECO:0000103, PECO:0000005, PECO:0000126PMID:9203579
FYPO:0001919fragmented nucleusMicroscopyT42A (T42A)amino_acid_mutationNot specifiedPECO:0000005PMID:9203579
FYPO:0001976normal cytoplasmic microtubulesMicroscopyspg1delta (deletion)deletionNot specifiedPECO:0000103, PECO:0000005, PECO:0000126PMID:9203579
FYPO:0001991inviable after spore germination, without cell divisionMicroscopyspg1delta (deletion)deletionNot specifiedPMID:9203579
FYPO:0002000septated mononucleate cellMicroscopyspg1+ (wild type)wild_typeOverexpressionPMID:9203579
MicroscopyQ69L (Q69L)amino_acid_mutationNot specifiedPMID:9203579
MicroscopyD125A (D125A)amino_acid_mutationNot specifiedPMID:9203579
FYPO:0002002multiseptate cell, septa groupedMicroscopyspg1+ (wild type)wild_typeOverexpressionPMID:9203579
MicroscopyQ69L (Q69L)amino_acid_mutationNot specifiedPMID:9203579
MicroscopyD125A (D125A)amino_acid_mutationNot specifiedPMID:9203579
FYPO:0002024inviable elongated multinucleate aseptate vegetative cellMicroscopyspg1delta (deletion)deletionNot specifiedPECO:0000005, PECO:0000103, PECO:0000126PMID:9203579
FYPO:0002061inviable vegetative cell populationMicroscopyspg1delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0003924GTPase activityTASPMID:9203579
GO:0005515protein bindingIPIcdc7PMID:15062098
IPIetd1PMID:19736319
GO:0005525GTP bindingICGO:0003924GO_REF:0000001
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000087M phase of mitotic cell cycleNASGO_REF:0000051
GO:0010973positive regulation of barrier septum assemblyIMPPMID:9203579
GO:0031028septation initiation signaling cascadeIGIcdc7PMID:9203579
GO:0034613cellular protein localization
has_input cdc7IMPPMID:11676915
has_input sid2IMPPMID:11676915
has_input sid1IMPPMID:11676915
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0005816spindle pole bodyIDAPMID:9203579
GO:0044732mitotic spindle pole bodyIDAPMID:16823372
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
113011781300889
213008421300634
313004801300397
413003561300282
513002421299707

References

Region Start End Reference
three_prime_UTR13001431299707PMID:21511999
three_prime_UTR13001431299707PMID:21511999
five_prime_UTR13011781301019PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00071 IPR001806 Pfam Small GTPase superfamily 12 175 18
PS51419 Prosite Profiles 6 198 9
G3DSA:3.40.50.300 Gene3D 5 181 279
SSF52540 SuperFamily 6 185 294
PIRSF037527 IPR017231 PIRSF Small GTPase superfamily, Tem1 1 198 1
PR00449 IPR001806 PRINTS Small GTPase superfamily 153 175 19
PR00449 IPR001806 PRINTS Small GTPase superfamily 52 74 19
PR00449 IPR001806 PRINTS Small GTPase superfamily 113 126 19
PR00449 IPR001806 PRINTS Small GTPase superfamily 34 50 19
PR00449 IPR001806 PRINTS Small GTPase superfamily 11 32 19
PTHR24073 hmmpanther 2 197 9
PTHR24073:SF258 hmmpanther 2 197 1
TIGR00231 IPR005225 tigrfam Small GTP-binding protein domain 10 172 35

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residue

Protein Properties

Ave. residue weight 113.85 Da
Charge 5.00
Isoelectric point 9.12
Molecular weight 22.54 kDa
Number of residues 198
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelNorthern assay evidencemitotic cell cycleconstantPMID:9203579
experimental evidencesingle-celled organism vegetative growth phasePECO:0000005,
PECO:0000014		population_widePMID:23101633
Northern assay evidencesingle-celled organism vegetative growth phasepresentPMID:9203579
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide4548.52PMID:23101633
RNA levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.28PMID:23101633
Western blot evidencemitotic cell cycleconstantPMID:9203579
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide1.3PMID:23101633
Western blot evidencesingle-celled organism vegetative growth phasepresentPMID:9203579
Complementation
DescriptionQualifierReference
is not functionally complemented by S. cerevisiae TEM1 PMID:9203579
Species Distribution
DescriptionQualifierReference
conserved in fungi
conserved in eukaryotes
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
mug129sequence orphan Negative GeneticPMID:22681890
clp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic LethalityPMID:11683392
Synthetic LethalityPMID:15857958
zrg17cation diffusion family zinc membrane transporter Zrg17 Negative GeneticPMID:22681890
cdc7serine/threonine protein kinase Cdc7 Dosage RescuePMID:9203579
Synthetic Lethality
Synthetic LethalityPMID:9649519
Dosage RescuePMID:10924454
dma1mitotic spindle checkpoint protein Dma1 Phenotypic EnhancementPMID:20980623
zfs1CCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism Synthetic RescuePMID:10462526
ace2transcription factor Ace2 Synthetic RescuePMID:16415366
cdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Synthetic LethalityPMID:9203579
Dosage RescuePMID:10924454
sid4SIN component scaffold protein Sid4 Synthetic LethalityPMID:9649519
pab1protein phosphatase regulatory subunit Pab1 Dosage RescuePMID:11707284
scw1RNA-binding protein Scw1 Synthetic RescuePMID:12242222
Synthetic RescuePMID:12796296
byr4two-component GAP Byr4 Dosage RescuePMID:9742395
sid2NDR kinase Sid2 Synthetic LethalityPMID:11271422
Dosage RescuePMID:10924454
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Synthetic RescuePMID:22267499
myo2myosin II heavy chain Synthetic Growth DefectPMID:11739799
SPAC343.06cphospholipid scramblase (predicted) Negative GeneticPMID:22681890
ran1serine/threonine protein kinase Ran1 Phenotypic EnhancementPMID:16787941
SPCC1840.09NAD dependent epimerase/dehydratase family protein Negative GeneticPMID:22681890
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Phenotypic EnhancementPMID:10503548
Phenotypic Suppression
mob1Sid2-Mob1 kinase complex regulatory subunit Mob1 Dosage RescuePMID:10769201
Synthetic Growth Defect
ppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Dosage RescuePMID:12354095
par1protein phosphatase regulatory subunit Par1 Synthetic RescuePMID:11707284
Phenotypic SuppressionPMID:11514436
Synthetic Rescue
etd1ethanol-hypersensitive mutant protein Etd1 Synthetic RescuePMID:19736319
cdc14SIN component Cdc14 Synthetic LethalityPMID:9203579
Synthetic LethalityPMID:9649519
cdc16two-component GAP Cdc16 Synthetic RescuePMID:11271422
Synthetic RescuePMID:9203579
clr2chromatin silencing protein Clr2 Negative GeneticPMID:22681890
rpl210260S ribosomal protein L21 (predicted) Negative GeneticPMID:22681890
erd1Erd1 homolog (predicted) Negative GeneticPMID:22681890
plo1Polo kinase Plo1 Dosage RescuePMID:10924454
cdc11SIN component scaffold protein, centriolin ortholog Cdc11 Synthetic LethalityPMID:9649519
Synthetic LethalityPMID:9203579
Synthetic Growth DefectPMID:23297348
Dosage RescuePMID:10924454
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
byr4two-component GAP Byr4 Affinity Capture-WesternPMID:10799520
Two-hybridPMID:9638658
Affinity Capture-WesternPMID:18252797
Two-hybridPMID:9742395
Reconstituted Complex
cdc7serine/threonine protein kinase Cdc7 Affinity Capture-WesternPMID:9420333
Affinity Capture-WesternPMID:9203579
Two-hybrid
Two-hybridPMID:16469735
Affinity Capture-Western
Reconstituted Complex
Affinity Capture-WesternPMID:18225957
etd1ethanol-hypersensitive mutant protein Etd1 Reconstituted ComplexPMID:19736319
cdc16two-component GAP Cdc16 Affinity Capture-WesternPMID:10799520
sid4SIN component scaffold protein Sid4 Co-localizationPMID:10805785
sad1spindle pole body protein Sad1 Co-localizationPMID:9420333
cdc11SIN component scaffold protein, centriolin ortholog Cdc11 Co-localizationPMID:11676915
Reconstituted ComplexPMID:15062098
Two-hybrid
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External References
Database Identifier Description
NBRP SPAC1565.06c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1565.06c Retrieval of eukaryotic orthologs
BioGrid SPAC1565.06c BioGRID Interaction Datasets
Bähler Lab SPAC1565.06c Cell Cycle Expression Profile
Bähler Lab SPAC1565.06c Meiosis/Sporulation Expression Profies
Bähler Lab SPAC1565.06c Pheromone response/mating expression profiles
Bähler Lab SPAC1565.06c Environmental stress expression profiles
Bähler Lab SPAC1565.06c Bähler Lab Transcriptome Viewer
Cyclebase SPAC1565.06c Cell Cycle Data
PInt SPAC1565.06c Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC1565.06c Entrez Gene
WikiGene2542761GTPase Spg1
Uniprot_genenameSPG1
EntrezGene2542761GTPase Spg1
SPD / RIKEN11/11H01Orfeome Localization Data
UniProtKB/SwissProtP87027Septum-promoting GTP-binding protein 1
ModBaseP87027Database of comparative protein structure models
Pfam Protein DomainsP87027Pfam Domain Arrangement
RefSeq PeptideNP_593285GTPase Spg1
RefSeq mRNANM_001018715972h- GTPase Spg1 (spg1), mRNA
European Nucleotide ArchiveCAA04846ENA Protein Mapping
European Nucleotide ArchiveCAA72985ENA Protein Mapping
European Nucleotide ArchiveCAB99273ENA Protein Mapping
UniParcUPI0000135E07UniProt Archive

Literature for spg1
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013