bud6 (SPAC15A10.16)

Gene Standard Namebud6 Characterisation Statuspublished
Systematic IDSPAC15A10.16 Feature Typeprotein coding
Synonymsaip3, fat1, SPAC15E1.01 Name Description
Productactin interacting protein 3 homolog Bud6 Product Size1385aa, 154.33 kDa
Genomic Location Chromosome I, 3716643-3720963 (4321nt); CDS:3716806-3720963 (4158nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003779actin bindingISOSGD:S000004311GO_REF:000002438
GO:0005515protein bindingIPImst1PMID:18505873865
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030036actin cytoskeleton organizationISOSGD:S000004311GO_REF:000002495
GO:0051519activation of bipolar cell growthIMPPMID:1151664411
GO:0071963establishment or maintenance of cell polarity regulating cell shapeICGO:0042815GO_REF:000000137
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030478actin capISOSGD:S000004311GO_REF:00000241
GO:0051286cell tipIDAPMID:20870879192
GO:0035838growing cell tip21
during pseudohyphal growthIDAPMID:20870879
GO:0035839non-growing cell tip8
during pseudohyphal growthIDAPMID:20870879
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopybud6ΔNullPMID:204732893751
Microscopybud6ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000644normal protein localization during vegetative growth119
affecting arf6Microscopybud6ΔNullPMID:18060866
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopybud6ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
FYPO affected by mutation in arf6 ADP-ribosylation factor, Arf family Arf6 PMID:18060866
FYPO affected by mutation in sec3 exocyst complex subunit Sec3 PMID:22891673
FYPO affected by mutation in tea1 cell end marker Tea1 PMID:21652630
GO localized by tea1 cell end marker Tea1 PMID:15827087
Ensembl transcript structure with UTRs, exons and introns


Exon Start End
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03915 Pfam IPR022782 Actin interacting protein 3, C-terminal 793 1225 1
SM00806 SMART IPR005613 Actin interacting protein 3 793 1225 1
PTHR22741:SF2 HMMPANTHER IPR027704 Bud site selection protein 6, fungi 6 1233 1
PTHR22741 HMMPANTHER 6 1233 1
Coil ncoils Rabaptin coiled-coil domain 1054 1075 968
Coil ncoils Rabaptin coiled-coil domain 492 520 968
Coil ncoils Rabaptin coiled-coil domain 1014 1035 968
Coil ncoils Rabaptin coiled-coil domain 596 620 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.43 Da
Charge -34.00
Isoelectric point 4.94
Molecular weight 154.33 kDa
Number of residues 1385

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
present during mitotic M phase
present during mitotic M phaseS312PMID:21712547
present during mitotic M phaseS634PMID:21712547
present during mitotic M phaseS636PMID:21712547
present during mitotic M phaseS361PMID:21712547
present during mitotic M phaseS362PMID:21712547
present during mitotic M phaseS309PMID:21712547
present during mitotic M phaseS303PMID:21712547
present during mitotic M phaseS520PMID:21712547
present during mitotic M phaseS1290PMID:21712547
present during mitotic M phaseS1328PMID:21712547
present during cellular response to thiabendazoleIDAS602PMID:18257517
present during mitotic M phaseS511PMID:21712547
present during mitotic M phaseS1247PMID:21712547
present during mitotic M phaseS516PMID:21712547
present during mitotic M phaseS512PMID:21712547
present during mitotic M phaseS342PMID:21712547
present during mitotic M phaseS359PMID:21712547
present during mitotic M phaseS631PMID:21712547
present during mitotic M phaseS602PMID:21712547
present during mitotic M phaseS295PMID:21712547
present during mitotic M phaseS293PMID:21712547
present during mitotic M phase
present during mitotic M phaseT347PMID:21712547
present during mitotic M phaseT334PMID:21712547
present during mitotic M phaseT340PMID:21712547
present during mitotic M phaseT349PMID:21712547
present during mitotic M phase
MOD:00696phosphorylated residueNASPMID:182575171922
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7770during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
7656during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
7287during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
6848during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
7463during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
1835.83during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
3469.08during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.4during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
1.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi only620
conserved in fungi4599
conserved in eukaryotes4514

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
fan1Fanconi-associated nuclease Fan1 Synthetic LethalityPMID:24192486
ryh1GTPase Ryh1 Synthetic Growth DefectPMID:18931302
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
mst1KAT5 family histone acetyltransferase Mst1 Two-hybridPMID:18505873
tea1cell end marker Tea1 Affinity Capture-WesternPMID:11516644
Affinity Capture-MSPMID:21652630
External References
Database Identifier Description
NBRP SPAC15A10.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC15A10.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC15A10.16 BioGRID Interaction Datasets
Expression Viewer SPAC15A10.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC15A10.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC15A10.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC15A10.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC15A10.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC15A10.16 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC15A10.16 Cell Cycle Data
GEO SPAC15A10.16 GEO profiles
PInt SPAC15A10.16 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC15A10.16 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN30/30F12Orfeome Localization Data
UniProtKB/SwissProtO13735Actin-interacting protein 3 homolog
ModBaseO13735Database of comparative protein structure models
STRINGO13735Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594302actin-interacting protein 3
RefSeq mRNANM_001019725972h- actin-interacting protein 3 (bud6), mRNA
European Nucleotide ArchiveCAB10112.1ENA Protein Mapping
UniParcUPI000012A554UniProt Archive

Literature for bud6

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014