bud6 (SPAC15A10.16)


Gene Standard Namebud6 Characterisation Statuspublished
Systematic IDSPAC15A10.16 Feature Typeprotein coding
Synonymsaip3, fat1, SPAC15E1.01 Name Description
Productactin interacting protein 3 homolog Bud6 Product Size1385aa, 154.33 kDa
Genomic Location Chromosome I, 3716643-3720963 (4321nt); CDS:3716806-3720963 (4158nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding38
Annotation ExtensionEvidenceWith/FromReference
protein binding859
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin cytoskeleton organization102
Annotation ExtensionEvidenceWith/FromReference
activation of bipolar cell growth11
Annotation ExtensionEvidenceWith/FromReference
establishment or maintenance of cell polarity regulating cell shape56
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cap1
Annotation ExtensionEvidenceWith/FromReference
cell tip194
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4209
Annotation ExtensionEvidenceWith/FromReference
growing cell tip24
Annotation ExtensionEvidenceWith/FromReference
non-growing cell tip9
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
slow vegetative cell population growthbud6ΔNull328
viable vegetative cell populationbud6ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
normal protein localization during vegetative growth193
affecting arf6bud6ΔNull
viable vegetative cell with normal cell morphologybud6ΔNull3094
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in tea1 cell end marker Tea1
GO localized by tea1 cell end marker Tea1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3716643..3720963
mRNA3716643..3720963
5' UTR3716643..3716805PMID:18641648
5' UTR3716643..3716805PMID:20118936
CDS3716806..3720963
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03915 Pfam IPR022782 Actin interacting protein 3, C-terminal 793 1225 1
SM00806 SMART IPR005613 Actin interacting protein 3 793 1225 1
PTHR22741 HMMPANTHER 5 1383 1
Coil ncoils Rabaptin coiled-coil domain 596 620 968
Coil ncoils Rabaptin coiled-coil domain 1054 1075 968
Coil ncoils Rabaptin coiled-coil domain 1014 1035 968
Coil ncoils Rabaptin coiled-coil domain 492 520 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.43 Da
Charge -34.00
Isoelectric point 4.94
Molecular weight 154.33 kDa
Number of residues 1385
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS631
present during mitotic M phaseS361
present during mitotic M phaseS520
present during mitotic M phaseS295
present during mitotic M phaseS362
present during mitotic M phaseS621
present during mitotic M phaseS312
S293, S295, S312, S362, S511
present during mitotic M phaseS303
present during mitotic M phaseS359
present during mitotic M phaseS309
present during mitotic M phaseS512
present during mitotic M phaseS1290
present during mitotic M phaseS636
present during mitotic M phaseS511
present during mitotic M phaseS634
present during mitotic M phaseS1247
present during mitotic M phaseS293
present during cellular response to thiabendazole, mitotic M phaseS602
present during mitotic M phaseS342
present during mitotic M phaseS1328
present during mitotic M phaseS516
Annotation ExtensionEvidenceResidueReference
experimental evidence S293 PMID:24763107
present during mitotic M phase experimental evidence S293 PMID:21712547
experimental evidence S295 PMID:24763107
present during mitotic M phase experimental evidence S295 PMID:21712547
present during mitotic M phase experimental evidence S303 PMID:21712547
present during mitotic M phase experimental evidence S309 PMID:21712547
experimental evidence S312 PMID:24763107
present during mitotic M phase experimental evidence S312 PMID:21712547
present during mitotic M phase experimental evidence S342 PMID:21712547
present during mitotic M phase experimental evidence S359 PMID:21712547
present during mitotic M phase experimental evidence S361 PMID:21712547
experimental evidence S362 PMID:24763107
present during mitotic M phase experimental evidence S362 PMID:21712547
experimental evidence S511 PMID:24763107
present during mitotic M phase experimental evidence S511 PMID:21712547
present during mitotic M phase experimental evidence S512 PMID:21712547
present during mitotic M phase experimental evidence S516 PMID:21712547
present during mitotic M phase experimental evidence S520 PMID:21712547
present during mitotic M phase experimental evidence S602 PMID:21712547
present during cellular response to thiabendazole IDA S602 PMID:18257517
present during mitotic M phase experimental evidence S621 PMID:21712547
present during mitotic M phase experimental evidence S631 PMID:21712547
present during mitotic M phase experimental evidence S634 PMID:21712547
present during mitotic M phase experimental evidence S636 PMID:21712547
present during mitotic M phase experimental evidence S1247 PMID:21712547
present during mitotic M phase experimental evidence S1290 PMID:21712547
present during mitotic M phase experimental evidence S1328 PMID:21712547
O-phospho-L-threonineT336 698
present during mitotic M phaseT340
present during mitotic M phaseT1294
present during mitotic M phaseT347
present during mitotic M phaseT334
present during mitotic M phaseT349
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T334 PMID:21712547
experimental evidence T336 PMID:24763107
present during mitotic M phase experimental evidence T340 PMID:21712547
present during mitotic M phase experimental evidence T347 PMID:21712547
present during mitotic M phase experimental evidence T349 PMID:21712547
present during mitotic M phase experimental evidence T1294 PMID:21712547
O4'-phospho-L-tyrosine 87
present during mitotic M phaseY1327
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence Y1327 PMID:21712547
phosphorylated residue 1927
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7770during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
7656during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
7287during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6848during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1835.83during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
7463during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3469.08during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.4during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only620
conserved in fungi4603
conserved in eukaryotes4514
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withmst1KAT5 family histone acetyltransferase Mst1 Two-hybridPMID:18505873
affinity capturestea1cell end marker Tea1 Affinity Capture-WesternPMID:11516644
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
synthetic lethal withfan1Fanconi-associated nuclease Fan1 Synthetic LethalityPMID:24192486
synthetic growth defect withryh1GTPase Ryh1 Synthetic Growth DefectPMID:18931302
negative genetic interaction withsgf73SAGA complex subunit Sgf73 Negative GeneticPMID:25076038
External References
Database Identifier Description
NBRP SPAC15A10.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC15A10.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC15A10.16 BioGRID Interaction Datasets
Expression Viewer SPAC15A10.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC15A10.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC15A10.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC15A10.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC15A10.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC15A10.16 Transcriptome Viewer (Bähler Lab)
GEO SPAC15A10.16 GEO profiles
PInt SPAC15A10.16 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC15A10.16 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC15A10.16 Fission yeast phenotypic data & analysis
Cyclebase SPAC15A10.16.1 Cell Cycle Data
SPD / RIKEN30/30F12Orfeome Localization Data
UniProtKB/SwissProtO13735Actin-interacting protein 3 homolog
ModBaseO13735Database of comparative protein structure models
STRINGO13735Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594302actin-interacting protein 3
RefSeq mRNANM_001019725972h- actin-interacting protein 3 (bud6), mRNA
European Nucleotide ArchiveCAB10112.1ENA Protein Mapping
UniParcUPI000012A554UniProt Archive

Literature for bud6

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015