bud6 (SPAC15A10.16)


Gene Standard Namebud6 Characterisation Statuspublished
Systematic IDSPAC15A10.16 Feature Typeprotein coding
Synonymsaip3, fat1, SPAC15E1.01 Name Description
Productactin interacting protein 3 homolog Bud6 Product Size1385aa, 154.33 kDa
Genomic Location Chromosome I, 3716643-3720963 (4321nt); CDS:3716806-3720963 (4158nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding38
Annotation ExtensionEvidenceWith/FromReference
cytoskeletal regulatory protein binding2
Annotation ExtensionEvidenceWith/FromReference
protein binding872
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin cytoskeleton organization99
Annotation ExtensionEvidenceWith/FromReference
actin filament bundle assembly11
Annotation ExtensionEvidenceWith/FromReference
activation of bipolar cell growth9
Annotation ExtensionEvidenceWith/FromReference
establishment or maintenance of cell polarity regulating cell shape54
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin cap1
Annotation ExtensionEvidenceWith/FromReference
cell tip204
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4212
Annotation ExtensionEvidenceWith/FromReference
growing cell tip28
Annotation ExtensionEvidenceWith/FromReference
non-growing cell tip9
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
slow vegetative cell population growthbud6Δ361
viable vegetative cell populationbud6Δ3844

Cell Phenotype

Term NameGenotypesCount
normal protein localization during vegetative growth254
affecting arf6bud6Δ
viable vegetative cell with normal cell morphologybud6Δ3100

FYPO Multi-allele Phenotypes

Cell Phenotype

Term NameGenotypes
normal protein localization to medial cortex during vegetative growth
affecting cdr2pom1Δ305N (10-303), bud6Δ
normal septum locationbud6Δ, pom1Δ305N (10-303)
viable small vegetative cellbud6Δ, pom1Δ305N (10-303)
Target Of
OntologyRelationshipGeneProduct
FYPO localization affected by mutation in alp4 gamma tubulin complex Spc97/GCP2 subunit Alp4
FYPO localization affected by mutation in tea1 cell end marker Tea1
GO localized by tea1 cell end marker Tea1
GO substrate of sst2 human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3716643..3720963
mRNA3716643..3720963
5' UTR3716643..3716805PMID:20118936
5' UTR3716643..3716805PMID:18641648
CDS3716806..3720963
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03915 Pfam IPR022782 Actin interacting protein 3, C-terminal 793 1225 1
SM00806 SMART IPR005613 Actin interacting protein 3 793 1225 1
PTHR22741 HMMPANTHER 263 435 1
PTHR22741 HMMPANTHER 705 1250 1
PTHR22741 HMMPANTHER 54 207 1
Coil ncoils Predicted coiled-coil protein (DUF2205) 596 619 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 1014 1034 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 1054 1074 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 492 519 1057

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.43 Da
Charge -34.00
Codon Adaptation Index 0.38
Isoelectric point 4.94
Molecular weight 154.33 kDa
Number of residues 1385
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1677
present during mitotic M phaseS511
present during mitotic M phaseS634
present during mitotic M phaseS631
present during mitotic M phaseS293
present during mitotic M phaseS303
present during cellular response to thiabendazole, mitotic M phaseS602
present during mitotic M phaseS309
present during mitotic M phaseS362
present during mitotic M phaseS342
present during mitotic M phaseS361
present during mitotic M phaseS1290
present during mitotic M phaseS636
present during mitotic M phaseS312
present during mitotic M phaseS359
present during mitotic M phaseS1247
present during mitotic M phaseS621
present during mitotic M phaseS516
present during mitotic M phaseS512
present during mitotic M phaseS1328
present during mitotic M phaseS295
present during mitotic M phaseS520
S293, S295, S312, S362, S511
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S511 PMID:21712547
present during mitotic M phase experimental evidence S634 PMID:21712547
present during mitotic M phase experimental evidence S631 PMID:21712547
present during mitotic M phase experimental evidence S293 PMID:21712547
present during mitotic M phase experimental evidence S303 PMID:21712547
present during cellular response to thiabendazole IDA S602 PMID:18257517
present during mitotic M phase experimental evidence S309 PMID:21712547
present during mitotic M phase experimental evidence S362 PMID:21712547
present during mitotic M phase experimental evidence S342 PMID:21712547
present during mitotic M phase experimental evidence S361 PMID:21712547
present during mitotic M phase experimental evidence S1290 PMID:21712547
present during mitotic M phase experimental evidence S636 PMID:21712547
present during mitotic M phase experimental evidence S312 PMID:21712547
present during mitotic M phase experimental evidence S359 PMID:21712547
present during mitotic M phase experimental evidence S1247 PMID:21712547
present during mitotic M phase experimental evidence S602 PMID:21712547
present during mitotic M phase experimental evidence S621 PMID:21712547
present during mitotic M phase experimental evidence S516 PMID:21712547
present during mitotic M phase experimental evidence S512 PMID:21712547
present during mitotic M phase experimental evidence S1328 PMID:21712547
present during mitotic M phase experimental evidence S295 PMID:21712547
present during mitotic M phase experimental evidence S520 PMID:21712547
experimental evidence S312 PMID:24763107
experimental evidence S293 PMID:24763107
experimental evidence S295 PMID:24763107
experimental evidence S511 PMID:24763107
experimental evidence S362 PMID:24763107
O-phospho-L-threonine 701
present during mitotic M phaseT1294
present during mitotic M phaseT347
present during mitotic M phaseT334
present during mitotic M phaseT340
present during mitotic M phaseT349
T336
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T1294 PMID:21712547
present during mitotic M phase experimental evidence T347 PMID:21712547
present during mitotic M phase experimental evidence T334 PMID:21712547
present during mitotic M phase experimental evidence T340 PMID:21712547
present during mitotic M phase experimental evidence T349 PMID:21712547
experimental evidence T336 PMID:24763107
O4'-phospho-L-tyrosine 88
present during mitotic M phaseY1327
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence Y1327 PMID:21712547
mannosylated residue 89
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence PMID:26644575
phosphorylated residue 1932
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
ubiquitinylated lysineK1299 512
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence K1299 PMID:26412298
IDA PMID:26412298
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for bud6 (SPAC15A10.16)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7770during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
7656during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
7287during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
6848during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1835.83during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
7463during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3469.08during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.4during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi only618
conserved in fungi4606
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC15A10.16 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturestea1cell end marker Tea1 Affinity Capture-WesternPMID:11516644
binds activation domain construct withSPBC16E9.15heat shock factor binding protein (predicted) Two-hybridPMID:26771498
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
binds DNA-binding domain construct withmst1KAT5 family histone acetyltransferase Mst1 Two-hybridPMID:18505873
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC15A10.16 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withsgf73SAGA complex subunit Sgf73 Negative GeneticPMID:25076038
synthetic growth defect withryh1GTPase Ryh1 Synthetic Growth DefectPMID:18931302
synthetic lethal withfan1Fanconi-associated nuclease Fan1 Synthetic LethalityPMID:24192486
External References
Database Identifier Description
NBRP SPAC15A10.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC15A10.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC15A10.16 BioGRID Interaction Datasets
Expression Viewer SPAC15A10.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC15A10.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC15A10.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC15A10.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC15A10.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC15A10.16 Transcriptome Viewer (Bähler Lab)
GEO SPAC15A10.16 GEO profiles
PInt SPAC15A10.16 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC15A10.16 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC15A10.16 Fission yeast phenotypic data & analysis
Cyclebase SPAC15A10.16.1 Cell Cycle Data
SPD / RIKEN30/30F12Orfeome Localization Data
UniProtKB/SwissProtO13735Actin-interacting protein 3 homolog
ModBaseO13735Database of comparative protein structure models
STRINGO13735Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594302actin-interacting protein 3
RefSeq mRNANM_001019725972h- actin-interacting protein 3 (bud6), mRNA
European Nucleotide ArchiveCAB10112.1ENA Protein Mapping
UniParcUPI000012A554UniProt Archive

Literature for bud6

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016