rho4 (SPAC16A10.04)


Gene Standard Namerho4 Characterisation Statuspublished
Systematic IDSPAC16A10.04 Feature Typeprotein coding
Synonyms Name Description
ProductRho family GTPase Rho4 Product Size203aa, 22.41 kDa
Genomic Location Chromosome I, 3086938-3088619 (1682nt); CDS:3087038-3088044 (1007nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTP binding113
Annotation ExtensionEvidenceWith/FromReference
GTPase activity100
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
barrier septum assembly41
Annotation ExtensionEvidenceWith/FromReference
cellular protein localization521
Annotation ExtensionEvidenceWith/FromReference
fungal-type cell wall organization or biogenesis105
Annotation ExtensionEvidenceWith/FromReference
microtubule cytoskeleton organization113
Annotation ExtensionEvidenceWith/FromReference
positive regulation of cell separation after cytokinesis2
Annotation ExtensionEvidenceWith/FromReference
primary cell septum disassembly3
Annotation ExtensionEvidenceWith/FromReference
regulation of actin cytoskeleton organization28
Annotation ExtensionEvidenceWith/FromReference
regulation of barrier septum assembly32
Annotation ExtensionEvidenceWith/FromReference
regulation of cell shape70
Annotation ExtensionEvidenceWith/FromReference
small GTPase mediated signal transduction102
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
barrier septum22
Annotation ExtensionEvidenceWith/FromReference
cell cortex168
Annotation ExtensionEvidenceWith/FromReference
cell division site309
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
decreased cell population growth on glucose carbon sourcerho4+286
increased cell population growth raterho4Δ247
normal cell population growth at high temperaturerho4Δ57
normal growth on glucose carbon sourcerho4Δ240
viable vegetative cell population3819
rho4Δ

Cell Phenotype

Term NameGenotypesCount
abnormal protein localization391
affecting sec6rho4Δ
abnormal septationrho4Δ268
abolished protein localization to medial cortex during vegetative growth21
affecting eng1rho4Δ
elongated multiseptate vegetative cellrho4Δ59
elongated telomeresrho4Δ170
increased protein binding16
affecting rho4 and sec8G23V (G23V)
normal protein localization232
affecting exo70rho4Δ
viable vegetative cell with normal cell morphologyrho4Δ3097

Multi-allele phenotypes


Population Phenotype

Term NameGenotypes
abolished cell population growth at high temperaturerho4Δ, sec8-1
decreased cell population growth at high temperatureexo70Δ, rho4Δ
decreased cell population growth on glucose carbon sourcerho4+, spn4Δ
sec8-1, rho4Δ
rho4+, spn1Δ
increased septation indexrho4Δ, rho3Δ
spn3Δ, rho4Δ
spn2Δ, rho4Δ
normal growth on glucose carbon sourcerho4Δ, exo70Δ

Cell Phenotype

Term NameGenotypes
abnormal protein localization
affecting exo70rho4Δ, rho3Δ
affecting sec8rho4Δ, rho3Δ
elongated multiseptate vegetative cellrho4Δ, sec8-1
multiseptate vegetative cellspn1Δ, rho4Δ
spn4Δ, rho4Δ
rho4Δ, rho3Δ
vegetative cell lysisrho4Δ, sec8-1
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in gef2 RhoGEF Gef2
FYPO affected by mutation in nod1 medial cortical node Gef2-related protein protein Nod1
FYPO affected by mutation in rho4 Rho family GTPase Rho4
GO input for gef2 RhoGEF Gef2
GO input for gef3 RhoGEF Gef3
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3086938..3087071, 3087127..3087168, 3087230..3087276, 3087384..3087587, 3087704..3087838, 3087895..3088619
Intron3087072..3087126, 3087169..3087229, 3087277..3087383, 3087588..3087703, 3087839..3087894
mRNA3086938..3088619
5' UTR3086938..3087037PMID:21511999
CDS3087038..3087071, 3087127..3087168, 3087230..3087276, 3087384..3087587, 3087704..3087838, 3087895..3088044
3' UTR3088045..3088619PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00071 Pfam IPR001806 Small GTPase superfamily 16 185 19
SM00174 SMART IPR003578 Small GTPase superfamily, Rho type 17 191 19
SM00175 SMART IPR003579 Small GTPase superfamily, Rab type 16 191 22
SM00173 SMART IPR020849 Small GTPase superfamily, Ras type 12 191 19
PS51420 Prosite Profiles IPR003578 Small GTPase superfamily, Rho type 8 189 6
PTHR24072:SF39 HMMPANTHER 1 203 1
PTHR24072 HMMPANTHER 1 203 7
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 11 185 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 16 186 294
PR00449 PRINTS 57 79 19
PR00449 PRINTS 38 54 19
PR00449 PRINTS 15 36 19
PR00449 PRINTS 166 188 19
PR00449 PRINTS 119 132 19
TIGR00231 tigrfam IPR005225 Small GTP-binding protein domain 14 183 35

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000741Rho familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000741

Protein Properties

Ave. residue weight 110.38 Da
Charge 3.50
Isoelectric point 8.26
Molecular weight 22.41 kDa
Number of residues 203
Modifications

Protein Modifications

Term NameResidueCount
farnesylated residue 13
Annotation ExtensionEvidenceResidueReference
ISS PB_REF:0000001
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3583.91during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
5747.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.9during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated433
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3421
conserved in vertebrates3396
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPAC16A10.04 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesscd1RhoGEF Scd1 Affinity Capture-WesternPMID:14637153
affinity capturessec8exocyst complex subunit Sec8 Affinity Capture-WesternPMID:25724972
affinity capturesexo70exocyst complex subunit Exo70 Affinity Capture-WesternPMID:25724972
affinity capturesspn3mitotic septin Spn3 Affinity Capture-WesternPMID:25724972
affinity captured bygef3RhoGEF Gef3 Affinity Capture-WesternPMID:25411334
forms complex withgef2RhoGEF Gef2 Reconstituted ComplexPMID:23966468
Genetic Interactions

Source: BioGRID

Load gene that interact geneitically with SPAC16A10.04 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withubp2ubiquitin C-terminal hydrolase Ubp2 Negative GeneticPMID:22681890
negative genetic interaction withSPCC16C4.106-phosphogluconolactonase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC713.07cvacuolar polyphosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpar1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
negative genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
synthetic growth defect withnda3tubulin beta Nda3 Synthetic Growth DefectPMID:12653963
synthetic growth defect withsid2NDR kinase Sid2 Synthetic Growth DefectPMID:16415366
synthetic growth defect withscw1RNA-binding protein Scw1 Synthetic Growth DefectPMID:25411334
synthetic growth defect withfor3formin For3 Synthetic Growth DefectPMID:25724972
synthetic growth defect withexo70exocyst complex subunit Exo70 Synthetic Growth DefectPMID:25411334
synthetic growth defect withsec8exocyst complex subunit Sec8 Synthetic Growth DefectPMID:25724972
synthetic growth defect withgef1Cdc42 RhoGEF Gef1 Synthetic Growth DefectPMID:25411334
inviable in presence of overexpressedeng1endo-1,3-beta-glucanase Eng1 Dosage LethalityPMID:16215171
inviable in presence of overexpressedagn1glucan endo-1,3-alpha-glucosidase Agn1 Dosage LethalityPMID:16215171
rescued byeng1endo-1,3-beta-glucanase Eng1 Phenotypic SuppressionPMID:16215171
rescued byagn1glucan endo-1,3-alpha-glucosidase Agn1 Phenotypic SuppressionPMID:16215171
positive genetic interaction withmph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
positive genetic interaction withiwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withhus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
phenotype enhanced byspn2mitotic and meiotic (sporulation) septin Spn2 Phenotypic EnhancementPMID:25724972
phenotype enhanced byspn1mitotic septin Spn1 Phenotypic EnhancementPMID:25724972
phenotype enhanced byspn4mitotic septin Spn4 Phenotypic EnhancementPMID:25724972
phenotype enhanced byrho3Rho family GTPase Rho3 Phenotypic EnhancementPMID:25724972
phenotype enhanced byspn3mitotic septin Spn3 Phenotypic EnhancementPMID:25724972
synthetically rescuescdc7serine/threonine protein kinase Cdc7 Synthetic RescuePMID:23966468
synthetically rescuescdc11SIN component scaffold protein, centriolin ortholog Cdc11 Synthetic RescuePMID:23966468
synthetically rescuessid2NDR kinase Sid2 Synthetic RescuePMID:23966468
External References
Database Identifier Description
NBRP SPAC16A10.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC16A10.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC16A10.04 BioGRID Interaction Datasets
Expression Viewer SPAC16A10.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC16A10.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC16A10.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC16A10.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC16A10.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC16A10.04 Transcriptome Viewer (Bähler Lab)
GEO SPAC16A10.04 GEO profiles
PInt SPAC16A10.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC16A10.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC16A10.04 Fission yeast phenotypic data & analysis
Cyclebase SPAC16A10.04.1 Cell Cycle Data
SPD / RIKEN16/16F09Orfeome Localization Data
UniProtKB/SwissProtQ874R1GTP-binding protein rho4
ModBaseQ874R1Database of comparative protein structure models
STRINGQ874R1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018257Rho family GTPase Rho4
RefSeq mRNANM_001019469972h- Rho family GTPase Rho4 (rho4), mRNA
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveCAD86931ENA Protein Mapping
European Nucleotide ArchiveCAD86931.2ENA Protein Mapping
UniParcUPI00002317A8UniProt Archive

Literature for rho4

Search: Europe PMC or PubMed

Release Version: PomBase:29_56 - 12 Nov 2015