rho4 (SPAC16A10.04)

Gene Standard Namerho4 Characterisation Statuspublished
Systematic IDSPAC16A10.04 Feature Typeprotein coding
Synonyms Name Description
ProductRho family GTPase Rho4 Product Size203aa, 22.41 kDa
Genomic Location Chromosome I, 3086938-3088619 (1682nt); CDS:3087038-3088044 (1007nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005525GTP bindingIEAUniProtKB-KW:KW-0342GO_REF:0000037113
GO:0003924GTPase activityIDAPMID:1879333861
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0034613cellular protein localization550
localizes agn1IMPPMID:16215171
localizes eng1IMPPMID:16215171
GO:0071852fungal-type cell wall organization or biogenesisTASPMID:12796297118
GO:0000226microtubule cytoskeleton organizationIMPPMID:12653963109
GO:0030994primary cell septum disassemblyTASPMID:127962973
GO:0032956regulation of actin cytoskeleton organizationIMPPMID:1265396329
GO:0032955regulation of barrier septum assemblyIMPPMID:1265396334
GO:0008360regulation of cell shapeIMPPMID:1265396382
GO:0007264small GTPase mediated signal transductionIEAIPR001806GO_REF:0000002139
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000935barrier septum15
during mitotic cell cycleIDAPMID:12653963
GO:0005938cell cortex160
during mitotic interphaseIDAPMID:12653963
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyrho4ΔNullPMID:204732893759
Microscopyrho4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopynot recorded (unrecorded)PMID:12653963

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000223elongated multiseptate cellMicroscopyrho4ΔNullPMID:1265396348
FYPO:0002019elongated telomeres158
expressivity FYPO_EXT:0000003Gel electrophoresis evidencerho4ΔNullPMID:20625380
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyrho4ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
FYPO affected by mutation in gef2 RhoGEF Gef2 PMID:23966468
FYPO affected by mutation in nod1 medial cortical node Gef2-related protein protein Nod1 PMID:23966468
GO input for gef2 RhoGEF Gef2 PMID:23966468
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00071 Pfam IPR001806 Small GTPase superfamily 16 185 18
SM00175 SMART IPR003579 Small GTPase superfamily, Rab type 16 191 22
SM00174 SMART IPR003578 Small GTPase superfamily, Rho type 17 191 19
SM00173 SMART IPR020849 Small GTPase superfamily, Ras type 12 191 19
PS51420 Prosite Profiles IPR003578 Small GTPase superfamily, Rho type 8 189 6
PTHR24072:SF39 HMMPANTHER 12 203 1
PTHR24072 HMMPANTHER 12 203 7 Gene3D 11 185 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 16 186 294
PR00449 PRINTS IPR001806 Small GTPase superfamily 15 36 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 166 188 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 38 54 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 119 132 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 57 79 19
TIGR00231 tigrfam IPR005225 Small GTP-binding protein domain 14 183 35

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000741Rho familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000741

Protein Properties

Ave. residue weight 110.38 Da
Charge 3.50
Isoelectric point 8.26
Molecular weight 22.41 kDa
Number of residues 203
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3583.91during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
5747.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.9during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
gene structure updated432
Species Distribution
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
cdc11SIN component scaffold protein, centriolin ortholog Cdc11 Phenotypic EnhancementPMID:23966468
agn1glucan endo-1,3-alpha-glucosidase Agn1 Dosage LethalityPMID:16215171
Phenotypic Suppression
mph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
eng1endo-1,3-beta-glucanase Eng1 Phenotypic SuppressionPMID:16215171
Dosage Lethality
nda3tubulin beta Nda3 Synthetic Growth DefectPMID:12653963
iwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
sid2NDR kinase Sid2 Synthetic Growth DefectPMID:16415366
Phenotypic SuppressionPMID:23966468
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
SPBC713.07cvacuolar polyphosphatase (predicted) Negative GeneticPMID:22681890
par1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
SPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
ubp2ubiquitin C-terminal hydrolase Ubp2 Negative GeneticPMID:22681890
rxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
SPCC16C4.106-phosphogluconolactonase (predicted) Negative GeneticPMID:22681890
cdc7serine/threonine protein kinase Cdc7 Phenotypic SuppressionPMID:23966468
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
gef2RhoGEF Gef2 Affinity Capture-WesternPMID:23966468
scd1RhoGEF Scd1 Affinity Capture-WesternPMID:14637153
External References
Database Identifier Description
NBRP SPAC16A10.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC16A10.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC16A10.04 BioGRID Interaction Datasets
Expression Viewer SPAC16A10.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC16A10.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC16A10.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC16A10.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC16A10.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC16A10.04 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC16A10.04 Cell Cycle Data
GEO SPAC16A10.04 GEO profiles
PInt SPAC16A10.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC16A10.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC16A10.04 Fission yeast phenotypic data & analysis
SPD / RIKEN16/16F09Orfeome Localization Data
UniProtKB/SwissProtQ874R1GTP-binding protein rho4
ModBaseQ874R1Database of comparative protein structure models
STRINGQ874R1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018257Rho family GTPase Rho4
RefSeq mRNANM_001019469972h- Rho family GTPase Rho4 (rho4), mRNA
European Nucleotide ArchiveCAD86931.2ENA Protein Mapping
UniParcUPI00002317A8UniProt Archive

Literature for rho4

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014