rho4 (SPAC16A10.04)


Gene Standard Namerho4 Characterisation Statuspublished
Systematic IDSPAC16A10.04 Feature Typeprotein coding
Synonyms Name Description
ProductRho family GTPase Rho4 Product Size203aa, 22.41 kDa
Genomic Location Chromosome I, 3086938-3088619 (1682nt); CDS:3087038-3088044 (1007nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
GTP binding113
Annotation ExtensionEvidenceWith/FromReference
GTPase activity62
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular protein localization553
Annotation ExtensionEvidenceWith/FromReference
fungal-type cell wall organization or biogenesis119
Annotation ExtensionEvidenceWith/FromReference
microtubule cytoskeleton organization107
Annotation ExtensionEvidenceWith/FromReference
primary cell septum disassembly3
Annotation ExtensionEvidenceWith/FromReference
regulation of actin cytoskeleton organization29
Annotation ExtensionEvidenceWith/FromReference
regulation of barrier septum assembly34
Annotation ExtensionEvidenceWith/FromReference
regulation of cell shape74
Annotation ExtensionEvidenceWith/FromReference
small GTPase mediated signal transduction154
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
barrier septum13
Annotation ExtensionEvidenceWith/FromReference
cell cortex168
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
increased cell population growth raterho4ΔNull246
viable vegetative cell populationrho4ΔNull3792
unnamed (unrecorded)

Cell Phenotype

Term NameAlleleExpressionCount
elongated multiseptate cellrho4ΔNull53
elongated telomeresrho4ΔNull167
viable vegetative cell with normal cell morphologyrho4ΔNull3092
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in gef2 RhoGEF Gef2 PMID:23966468
FYPO affected by mutation in nod1 medial cortical node Gef2-related protein protein Nod1 PMID:23966468
GO input for gef2 RhoGEF Gef2 PMID:23966468
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3086938..3087071, 3087127..3087168, 3087230..3087276, 3087384..3087587, 3087704..3087838, 3087895..3088619
Introns3087072..3087126, 3087169..3087229, 3087277..3087383, 3087588..3087703, 3087839..3087894
mRNA3086938..3088619
5' UTR3086938..3087037PMID:21511999
CDS3087038..3087071, 3087127..3087168, 3087230..3087276, 3087384..3087587, 3087704..3087838, 3087895..3088044
3' UTR3088045..3088619PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00071 Pfam IPR001806 Small GTPase superfamily 16 185 18
SM00175 SMART IPR003579 Small GTPase superfamily, Rab type 16 191 22
SM00174 SMART IPR003578 Small GTPase superfamily, Rho type 17 191 19
SM00173 SMART IPR020849 Small GTPase superfamily, Ras type 12 191 19
PS51420 Prosite Profiles IPR003578 Small GTPase superfamily, Rho type 8 189 6
PTHR24072:SF39 HMMPANTHER 7 191 1
PTHR24072 HMMPANTHER 7 191 7
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 11 185 288
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 16 186 294
PR00449 PRINTS IPR001806 Small GTPase superfamily 57 79 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 38 54 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 15 36 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 166 188 19
PR00449 PRINTS IPR001806 Small GTPase superfamily 119 132 19
TIGR00231 tigrfam IPR005225 Small GTP-binding protein domain 14 183 35

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000741Rho familyTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000741

Protein Properties

Ave. residue weight 110.38 Da
Charge 3.50
Isoelectric point 8.26
Molecular weight 22.41 kDa
Number of residues 203
Modifications

Protein Modifications

Term NameResidueCount
farnesylated residue 13
Annotation ExtensionEvidenceResidueReference
ISS PMID:
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3583.91during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
5747.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated432
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bygef2RhoGEF Gef2 Affinity Capture-WesternPMID:23966468
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
affinity capturesscd1RhoGEF Scd1 Affinity Capture-WesternPMID:14637153
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
inviable in presence of overexpressedagn1glucan endo-1,3-alpha-glucosidase Agn1 Dosage LethalityPMID:16215171
rescued byagn1glucan endo-1,3-alpha-glucosidase Agn1 Phenotypic SuppressionPMID:16215171
enhances phenotype ofcdc11SIN component scaffold protein, centriolin ortholog Cdc11 Phenotypic EnhancementPMID:23966468
rescuescdc7serine/threonine protein kinase Cdc7 Phenotypic SuppressionPMID:23966468
rescued byeng1endo-1,3-beta-glucanase Eng1 Phenotypic SuppressionPMID:16215171
inviable in presence of overexpressedeng1endo-1,3-beta-glucanase Eng1 Dosage LethalityPMID:16215171
positive genetic interaction withhus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
positive genetic interaction withiwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withmph1dual specificity protein kinase Mph1 Positive GeneticPMID:22681890
synthetic growth defect withnda3tubulin beta Nda3 Synthetic Growth DefectPMID:12653963
negative genetic interaction withpar1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:22681890
negative genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
synthetic growth defect withsid2NDR kinase Sid2 Synthetic Growth DefectPMID:16415366
rescuessid2NDR kinase Sid2 Phenotypic SuppressionPMID:23966468
positive genetic interaction withSPAC1952.06cspliceosomal complex subunit (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPBC713.07cvacuolar polyphosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC16C4.106-phosphogluconolactonase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withubp2ubiquitin C-terminal hydrolase Ubp2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC16A10.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC16A10.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC16A10.04 BioGRID Interaction Datasets
Expression Viewer SPAC16A10.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC16A10.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC16A10.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC16A10.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC16A10.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC16A10.04 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC16A10.04 Cell Cycle Data
GEO SPAC16A10.04 GEO profiles
PInt SPAC16A10.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC16A10.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC16A10.04 Fission yeast phenotypic data & analysis
SPD / RIKEN16/16F09Orfeome Localization Data
UniProtKB/SwissProtQ874R1GTP-binding protein rho4
ModBaseQ874R1Database of comparative protein structure models
STRINGQ874R1Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018257Rho family GTPase Rho4
RefSeq mRNANM_001019469972h- Rho family GTPase Rho4 (rho4), mRNA
European Nucleotide ArchiveCAD86931.2ENA Protein Mapping
UniParcUPI00002317A8UniProt Archive

Literature for rho4

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015