vph1 (SPAC16E8.07c)


Gene Standard Namevph1 Characterisation Statusbiological_role_inferred
Systematic IDSPAC16E8.07c Feature Typeprotein coding
Synonyms Name Description
ProductV-type ATPase V0 subunit a (predicted) Product Size831aa, 94.16 kDa
Genomic Location Chromosome I, 3513032-3510331 (2702nt); CDS:3513019-3510419 (2601nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
proton-transporting ATPase activity, rotational mechanism29
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ATP hydrolysis coupled proton transport17
Annotation ExtensionEvidenceWith/FromReference
hydrogen ion transmembrane transport43
Annotation ExtensionEvidenceWith/FromReference
vacuolar acidification17
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum596
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
vacuolar proton-transporting V-type ATPase, V0 domain6
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationvph1ΔNull1428

Cell Phenotype

Term NameAlleleExpressionCount
inviable sporevph1ΔNull468
inviable vegetative cell with abnormal cell shapevph1ΔNull482
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3513032..3513001, 3512948..3512848, 3512794..3510331
Introns3512847..3512795
mRNA3513032..3510331
5' UTR3513032..3513020PMID:21511999
CDS3513019..3513001, 3512948..3512848, 3512794..3510419
3' UTR3510418..3510331PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01496 Pfam IPR002490 V-type ATPase, V0 complex, 116kDa subunit family 27 827 1
TMhelix TMHMM 469 491 959
TMhelix TMHMM 434 456 959
TMhelix TMHMM 649 671 959
TMhelix TMHMM 769 791 959
TMhelix TMHMM 554 576 959
TMhelix TMHMM 583 605 959
PTHR11629:SF63 HMMPANTHER 2 831 1
PTHR11629 HMMPANTHER IPR002490 V-type ATPase, V0 complex, 116kDa subunit family 2 831 1
Coil ncoils Rabaptin coiled-coil domain 98 126 968
PIRSF001293 PIRSF IPR026028 ATPase, V0 complex, subunit 116kDa, eukaryotic 1 831 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.31 Da
Charge -13.50
Isoelectric point 5.40
Molecular weight 94.16 kDa
Number of residues 831
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
31132during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
29374during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
24807during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
33205during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
14259.79during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
28767during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
21408.75during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6.9during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
renal tubular acidosis2
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999432
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3430
conserved in vertebrates3405
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byhhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
External References
Database Identifier Description
NBRP SPAC16E8.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC16E8.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC16E8.07c BioGRID Interaction Datasets
Expression Viewer SPAC16E8.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC16E8.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC16E8.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC16E8.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC16E8.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC16E8.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC16E8.07c Cell Cycle Data
GEO SPAC16E8.07c GEO profiles
PInt SPAC16E8.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC16E8.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC16E8.07c Fission yeast phenotypic data & analysis
SPD / RIKEN28/28H06Orfeome Localization Data
UniProtKB/SwissProtO13742V-type proton ATPase subunit a
ModBaseO13742Database of comparative protein structure models
STRINGO13742Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594219V-type ATPase V0 subunit a (predicted)
RefSeq mRNANM_001019642972h- V-type ATPase V0 subunit a (predicted) (vph1), mRNA
European Nucleotide ArchiveCAB11035.2ENA Protein Mapping
UniParcUPI000228F417UniProt Archive

Literature for vph1

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015