vph1 (SPAC16E8.07c)


Gene Standard Namevph1 Characterisation Statusbiological_role_inferred
Systematic IDSPAC16E8.07c Feature Typeprotein coding
Synonyms Name Description
ProductV-type ATPase V0 subunit a (predicted) Product Size831aa, 94.16 kDa
Genomic Location Chromosome I, 3513032-3510331 (2702nt); CDS:3513019-3510419 (2601nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingICGO:0046933GO_REF:0000001559
GO:0046961proton-transporting ATPase activity, rotational mechanismISOSGD:S000005796GO_REF:000002429
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0015991ATP hydrolysis coupled proton transportIEAIPR002490GO_REF:000000217
GO:1902600hydrogen ion transmembrane transportISOSGD:S000005796GO_REF:000002443
GO:0007035vacuolar acidificationISOSGD:S000005796PMID:1629172523
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005783endoplasmic reticulumIDAPMID:16823372594
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371016
GO:0000220vacuolar proton-transporting V-type ATPase, V0 domainISOSGD:S000005796GO_REF:00000246
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyvph1ΔNullPMID:204732891309
Microscopyvph1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002151inviable sporeMicroscopyvph1ΔNullPECO:0000005, PECO:0000137PMID:23697806447
FYPO:0002451inviable vegetative cell with abnormal cell shapeMicroscopyvph1ΔNullPECO:0000005, PECO:0000137PMID:23697806441
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
135130323513001
235129483512848
335127943510331

UTRs

Region Start End Reference
three_prime_UTR35104183510331PMID:21511999
five_prime_UTR35130323513020PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01496 Pfam IPR002490 V-type ATPase, V0 complex, subunit 116kDa 27 827 1
TMhelix TMHMM 554 576 959
TMhelix TMHMM 583 605 959
TMhelix TMHMM 434 456 959
TMhelix TMHMM 769 791 959
TMhelix TMHMM 649 671 959
TMhelix TMHMM 469 491 959
PTHR11629:SF28 HMMPANTHER 1 831 1
PTHR11629 HMMPANTHER IPR002490 V-type ATPase, V0 complex, subunit 116kDa 1 831 1
Coil ncoils Rabaptin coiled-coil domain 98 126 975
PIRSF001293 PIRSF IPR026028 ATPase, V0 complex, subunit 116kDa, eukaryotic 2 831 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.31 Da
Charge -13.50
Isoelectric point 5.40
Molecular weight 94.16 kDa
Number of residues 831
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
21408.75during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
14259.79during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
6.9during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
renal tubular acidosis2
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:21511999426
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3394
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
External References
Database Identifier Description
NBRP SPAC16E8.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC16E8.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC16E8.07c BioGRID Interaction Datasets
Expression Viewer SPAC16E8.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC16E8.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC16E8.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC16E8.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC16E8.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC16E8.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC16E8.07c Cell Cycle Data
GEO SPAC16E8.07c GEO profiles
PInt SPAC16E8.07c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542310V-type ATPase subunit a
EntrezGene2542310V-type ATPase subunit a
SPD / RIKEN28/28H06Orfeome Localization Data
UniProtKB/SwissProtO13742V-type proton ATPase subunit a
ModBaseO13742Database of comparative protein structure models
StringO13742Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594219V-type ATPase subunit a
RefSeq mRNANM_001019642972h- V-type ATPase subunit a (vph1), mRNA
European Nucleotide ArchiveCAB11035ENA Protein Mapping
UniParcUPI000228F417UniProt Archive

Literature for vph1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014