vph1 (SPAC16E8.07c)

Gene Standard Namevph1 Characterisation Statusbiological_role_inferred
Systematic IDSPAC16E8.07c Feature Typeprotein coding
Synonyms Name Description
ProductV-type ATPase V0 subunit a (predicted) Product Size831aa, 94.16 kDa
Genomic Location Chromosome I, 3513032-3510331 (2702nt); CDS:3513019-3510419 (2601nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingICGO:0046933GO_REF:0000001558
GO:0046961proton-transporting ATPase activity, rotational mechanismISOSGD:S000005796GO_REF:000002429
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0015991ATP hydrolysis coupled proton transportIEAIPR002490GO_REF:000000217
GO:1902600hydrogen ion transmembrane transportISOSGD:S000005796GO_REF:000002443
GO:0007035vacuolar acidificationISOSGD:S000005796PMID:1629172522
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005783endoplasmic reticulumIDAPMID:16823372594
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371018
GO:0000220vacuolar proton-transporting V-type ATPase, V0 domainISOSGD:S000005796GO_REF:00000246
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopyvph1ΔNullPMID:204732891338
Microscopyvph1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002151inviable sporeMicroscopyvph1ΔNullPECO:0000005, PECO:0000137PMID:23697806465
FYPO:0002451inviable vegetative cell with abnormal cell shapeMicroscopyvph1ΔNullPECO:0000005, PECO:0000137PMID:23697806460
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01496 Pfam IPR002490 V-type ATPase, V0 complex, subunit 116kDa 27 827 1
TMhelix TMHMM 583 605 959
TMhelix TMHMM 554 576 959
TMhelix TMHMM 434 456 959
TMhelix TMHMM 769 791 959
TMhelix TMHMM 649 671 959
TMhelix TMHMM 469 491 959
PTHR11629 HMMPANTHER IPR002490 V-type ATPase, V0 complex, subunit 116kDa 1 831 1
PTHR11629:SF28 HMMPANTHER 1 831 1
Coil ncoils Rabaptin coiled-coil domain 98 126 968
PIRSF001293 PIRSF IPR026028 ATPase, V0 complex, subunit 116kDa, eukaryotic 2 831 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.31 Da
Charge -13.50
Isoelectric point 5.40
Molecular weight 94.16 kDa
Number of residues 831
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
31132during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
29374during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
24807during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
33205during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
28767during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
14259.79during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
21408.75during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6.9during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
1.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Disease Association
renal tubular acidosis2
gene structure updated PMID:21511999432
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
External References
Database Identifier Description
NBRP SPAC16E8.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC16E8.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC16E8.07c BioGRID Interaction Datasets
Expression Viewer SPAC16E8.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC16E8.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC16E8.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC16E8.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC16E8.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC16E8.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC16E8.07c Cell Cycle Data
GEO SPAC16E8.07c GEO profiles
PInt SPAC16E8.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC16E8.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC16E8.07c Fission yeast phenotypic data & analysis
SPD / RIKEN28/28H06Orfeome Localization Data
UniProtKB/SwissProtO13742V-type proton ATPase subunit a
ModBaseO13742Database of comparative protein structure models
STRINGO13742Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594219V-type ATPase V0 subunit a (predicted)
RefSeq mRNANM_001019642972h- V-type ATPase V0 subunit a (predicted) (vph1), mRNA
European Nucleotide ArchiveCAB11035.2ENA Protein Mapping
UniParcUPI000228F417UniProt Archive

Literature for vph1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014