vph1 (SPAC16E8.07c)

Gene Standard Namevph1 Characterisation Statusbiological role inferred
Systematic IDSPAC16E8.07c Feature Typeprotein coding
Synonyms Name Description
ProductV-type ATPase V0 subunit a (predicted) Product Size831aa, 94.16 kDa
Genomic Location Chromosome I, 3513032-3510331 (2702nt); CDS:3513019-3510419 (2601nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding551
Annotation ExtensionEvidenceWith/FromReference
proton-transporting ATPase activity, rotational mechanism27
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ATP hydrolysis coupled proton transport15
Annotation ExtensionEvidenceWith/FromReference
vacuolar acidification17
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum602
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane969
Annotation ExtensionEvidenceWith/FromReference
vacuolar proton-transporting V-type ATPase, V0 domain6
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationvph1Δ1468

Cell Phenotype

Term NameGenotypesCount
inviable sporevph1Δ476
inviable vegetative cell with abnormal cell shapevph1Δ793
Target Of
GO substrate of sst2 human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3513032..3513001, 3512948..3512848, 3512794..3510331
Intron3513000..3512949, 3512847..3512795
5' UTR3513032..3513020PMID:21511999
CDS3513019..3513001, 3512948..3512848, 3512794..3510419
3' UTR3510418..3510331PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01496 Pfam IPR002490 V-type ATPase, V0 complex, 116kDa subunit family 29 826 1
TMhelix TMHMM 469 491 950
TMhelix TMHMM 583 605 950
TMhelix TMHMM 434 456 950
TMhelix TMHMM 649 671 950
TMhelix TMHMM 554 576 950
TMhelix TMHMM 769 791 950
PTHR11629 HMMPANTHER IPR002490 V-type ATPase, V0 complex, 116kDa subunit family 2 831 1
PTHR11629:SF64 HMMPANTHER 2 831 1
Coil ncoils Predicted coiled-coil protein (DUF2205) 98 125 1049

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.31 Da
Charge -13.50
Codon Adaptation Index 0.49
Isoelectric point 5.40
Molecular weight 94.16 kDa
Number of residues 831
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for vph1 (SPAC16E8.07c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
31132during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
29374during GO:0000084PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
24807during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
33205during GO:0000087PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
14259.79during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
28767during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
21408.75during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
6.9during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
1.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Disease Association
renal tubular acidosis2
congenital disorders of glycosylation30
metabolic disorders143
carbohydrate metabolism disorders11
gene structure updated PMID:21511999435
Taxonomic Conservation
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in bacteria1005
conserved in metazoa3498
conserved in vertebrates3473

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC16E8.07c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
External References
Database Identifier Description
NBRP SPAC16E8.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC16E8.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC16E8.07c BioGRID Interaction Datasets
Expression Viewer SPAC16E8.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC16E8.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC16E8.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC16E8.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC16E8.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC16E8.07c Transcriptome Viewer (Bähler Lab)
GEO SPAC16E8.07c GEO profiles
PInt SPAC16E8.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC16E8.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC16E8.07c Fission yeast phenotypic data & analysis
Cyclebase SPAC16E8.07c.1 Cell Cycle Data
SPD / RIKEN28/28H06Orfeome Localization Data
UniProtKB/SwissProtO13742V-type proton ATPase subunit a
ModBaseO13742Database of comparative protein structure models
STRINGO13742Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594219V-type ATPase V0 subunit a (predicted)
RefSeq mRNANM_001019642972h- V-type ATPase V0 subunit a (predicted) (vph1), mRNA
European Nucleotide ArchiveCAB11035.2ENA Protein Mapping
UniParcUPI000228F417UniProt Archive

Literature for vph1

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017