sua7 (SPAC16E8.16)

Gene Standard Namesua7 Characterisation Statuspublished
Systematic IDSPAC16E8.16 Feature Typeprotein coding
Synonyms Name Description
Producttranscription factor TFIIB Product Size340aa, 36.69 kDa
Genomic Location Chromosome I, 3529970-3532143 (2174nt); CDS:3530337-3531503 (1167nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
RNA polymerase II transcription factor activity, TBP-class protein binding1
Annotation ExtensionEvidenceWith/FromReference
zinc ion binding239
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
regulation of transcription from RNA polymerase II promoter297
Annotation ExtensionEvidenceWith/FromReference
transcription initiation from RNA polymerase II promoter37
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationsua7Δ1451

Cell Phenotype

Term NameGenotypesCount
inviable after spore germination with normal, unseptated germ tube morphologysua7Δ237
inviable sporesua7Δ476
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3529970..3530470, 3530615..3532143
5' UTR3529970..3530336AU012157
CDS3530337..3530470, 3530615..3531503
3' UTR3531504..3532143PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
SM00385 SMART IPR013763 Cyclin-like 234 315 13
SM00385 SMART IPR013763 Cyclin-like 123 204 13
PS51134 Prosite Profiles IPR013137 Zinc finger, TFIIB-type 16 49 2
PS00782 Prosite Patterns IPR023486 Transcription factor TFIIB, conserved site 157 172 1
PTHR11618 HMMPANTHER IPR000812 Transcription factor TFIIB 18 321 2
1.10.472.10 Gene3D IPR013763 Cyclin-like 232 327 13 Gene3D OsmC/Ohr family 14 65 7
1.10.472.10 Gene3D IPR013763 Cyclin-like 121 208 13
SSF57783 SuperFamily 14 65 8
SSF47954 SuperFamily IPR013763 Cyclin-like 231 326 15
SSF47954 SuperFamily IPR013763 Cyclin-like 117 207 15
PR00685 PRINTS IPR000812 Transcription factor TFIIB 269 285 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 191 206 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 301 315 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 165 184 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 71 92 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 235 253 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 56 69 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 34 54 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 107.90 Da
Charge 9.00
Codon Adaptation Index 0.42
Isoelectric point 8.76
Molecular weight 36.69 kDa
Number of residues 340

Protein Modifications

Term NameResidueCount
ubiquitinylated lysineK104 512
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence K104 PMID:26412298
IDA PMID:26412298
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for sua7 (SPAC16E8.16)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5139during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
3408during GO:0000084PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
4922during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
5742during GO:0000087PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
855.06during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
4988during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
1.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in archaea238

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC16E8.16 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withgrh1human GRASP protein family Golgi protein (predicted) Two-hybridPMID:26771498
forms complex withtfg3transcription factor TFIIF complex subunit Tfg3 Reconstituted ComplexPMID:15616156
External References
Database Identifier Description
NBRP SPAC16E8.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC16E8.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC16E8.16 BioGRID Interaction Datasets
Expression Viewer SPAC16E8.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC16E8.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC16E8.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC16E8.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC16E8.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC16E8.16 Transcriptome Viewer (Bähler Lab)
GEO SPAC16E8.16 GEO profiles
PInt SPAC16E8.16 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC16E8.16 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC16E8.16 Fission yeast phenotypic data & analysis
Cyclebase SPAC16E8.16.1 Cell Cycle Data
SPD / RIKEN18/18D10Orfeome Localization Data
UniProtKB/SwissProtO13749Transcription initiation factor IIB
ModBaseO13749Database of comparative protein structure models
STRINGO13749Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594229transcription factor TFIIB
RefSeq mRNANM_001019652972h- transcription factor TFIIB (SPAC16E8.16), mRNA

Literature for sua7

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016