Gene Standard NameUnassigned Characterisation Statuspublished
Systematic IDSPAC16E8.16 Feature Typeprotein coding
Synonyms Name Description
Producttranscription factor TFIIB Product Size340aa, 36.69 kDa
Genomic Location Chromosome I, 3529970-3532143 (2174nt); CDS:3530337-3531503 (1167nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0008270zinc ion bindingIEAIPR000812GO_REF:0000002248
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006355regulation of transcription, DNA-templatedIEAIPR000812GO_REF:0000002379
GO:0006367transcription initiation from RNA polymerase II promoterIDAPMID:1235932933
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopySPAC16E8.16ΔNullPECO:0000005, PECO:0000137PMID:236978061315

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000311inviable after spore germination with normal, unseptated germ tube morphology237
penetrance FYPO_EXT:0000003MicroscopySPAC16E8.16ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0002151inviable spore457
penetrance FYPO_EXT:0000001MicroscopySPAC16E8.16ΔNullPECO:0000005, PECO:0000137PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF08271 Pfam IPR013137 Zinc finger, TFIIB-type 18 62 2
PF00382 Pfam IPR013150 Transcription factor TFIIB, cyclin-like domain 125 195 2
PF00382 Pfam IPR013150 Transcription factor TFIIB, cyclin-like domain 237 306 2
SM00385 SMART IPR013763 Cyclin-like 123 204 13
SM00385 SMART IPR013763 Cyclin-like 234 315 13
PS51134 Prosite Profiles IPR013137 Zinc finger, TFIIB-type 16 49 2
PS00782 Prosite Patterns IPR023486 Transcription factor TFIIB, conserved site 157 172 1
PTHR11618 HMMPANTHER IPR000812 Transcription factor TFIIB 4 323 2
1.10.472.10 Gene3D IPR013763 Cyclin-like 232 327 13
1.10.472.10 Gene3D IPR013763 Cyclin-like 121 208 13 Gene3D 14 65 7
SSF47954 SuperFamily IPR013763 Cyclin-like 117 207 15
SSF47954 SuperFamily IPR013763 Cyclin-like 231 326 15
SSF57783 SuperFamily 14 65 8
PR00685 PRINTS IPR000812 Transcription factor TFIIB 191 206 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 56 69 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 165 184 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 71 92 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 34 54 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 301 315 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 235 253 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 269 285 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 107.90 Da
Charge 9.00
Isoelectric point 8.76
Molecular weight 36.69 kDa
Number of residues 340
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
4988during GO:0072690PECO:0000005,
mass spectrometry evidencePMID:24763107
5139during GO:0000080PECO:0000005,
mass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
experimental evidencePMID:23101633
855.06during GO:0072690PECO:0000014,
experimental evidencePMID:23101633
3408during GO:0000084PECO:0000005,
mass spectrometry evidencePMID:24763107
4922during GO:0000085PECO:0000005,
mass spectrometry evidencePMID:24763107
5742during GO:0000087PECO:0000005,
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
experimental evidencePMID:23101633
2.6during GO:0072690PECO:0000014,
experimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in archaea237

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tfg3transcription factor TFIIF complex subunit Tfg3 Reconstituted ComplexPMID:15616156
External References
Database Identifier Description
NBRP SPAC16E8.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC16E8.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC16E8.16 BioGRID Interaction Datasets
Expression Viewer SPAC16E8.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC16E8.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC16E8.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC16E8.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC16E8.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC16E8.16 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC16E8.16 Cell Cycle Data
GEO SPAC16E8.16 GEO profiles
PInt SPAC16E8.16 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542339transcription factor TFIIB
EntrezGene2542339transcription factor TFIIB
SPD / RIKEN18/18D10Orfeome Localization Data
UniProtKB/SwissProtO13749Transcription initiation factor IIB
ModBaseO13749Database of comparative protein structure models
STRINGO13749Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594229transcription factor TFIIB
RefSeq mRNANM_001019652972h- transcription factor TFIIB (SPAC16E8.16), mRNA
European Nucleotide ArchiveCAB11044ENA Protein Mapping
European Nucleotide ArchiveCAB11044.1ENA Protein Mapping
UniParcUPI0000136C2FUniProt Archive

Literature for SPAC16E8.16

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014