SPAC16E8.16


Gene Standard NameUnassigned Characterisation Statuspublished
Systematic IDSPAC16E8.16 Feature Typeprotein coding
Synonyms Name Description
Producttranscription factor TFIIB Product Size340aa, 36.69 kDa
Genomic Location Chromosome I, 3529970-3532143 (2174nt); CDS:3530337-3531503 (1167nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0008270zinc ion bindingIEAIPR000812GO_REF:0000002248
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006355regulation of transcription, DNA-templatedIEAIPR000812GO_REF:0000002395
GO:0006367transcription initiation from RNA polymerase II promoterIDAPMID:1235932933
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005634nucleusIDAPMID:168233722730
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopySPAC16E8.16ΔNullPMID:204732891309
MicroscopySPAC16E8.16ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000311inviable after spore germination with normal, unseptated germ tube morphologyMicroscopySPAC16E8.16ΔNullPECO:0000005, PECO:0000137PMID:23697806237
penetrance FYPO_EXT:0000003
FYPO:0002151inviable sporeMicroscopySPAC16E8.16ΔNullPECO:0000005, PECO:0000137PMID:23697806447
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
135299703530470
235306153532143

UTRs

Region Start End Reference
three_prime_UTR35315043532143PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00382 Pfam IPR013150 Transcription factor TFIIB, cyclin-like domain 237 306 2
PF00382 Pfam IPR013150 Transcription factor TFIIB, cyclin-like domain 125 195 2
PF08271 Pfam IPR013137 Zinc finger, TFIIB-type 18 62 2
SM00385 SMART IPR013763 Cyclin-like 234 315 13
SM00385 SMART IPR013763 Cyclin-like 123 204 13
PS51134 Prosite Profiles IPR013137 Zinc finger, TFIIB-type 16 49 2
PS00782 Prosite Patterns IPR023486 Transcription factor TFIIB, conserved site 157 172 1
PTHR11618 HMMPANTHER IPR000812 Transcription factor TFIIB 4 328 2
G3DSA:2.20.25.10 Gene3D 14 65 7
G3DSA:1.10.472.10 Gene3D IPR013763 232 327 13
G3DSA:1.10.472.10 Gene3D IPR013763 121 208 13
SSF47954 SuperFamily IPR013763 231 326 15
SSF57783 SuperFamily 14 65 8
SSF47954 SuperFamily IPR013763 117 207 15
PR00685 PRINTS IPR000812 Transcription factor TFIIB 269 285 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 235 253 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 34 54 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 301 315 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 71 92 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 165 184 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 56 69 2
PR00685 PRINTS IPR000812 Transcription factor TFIIB 191 206 2

View domain organization at Pfam

Protein Properties

Ave. residue weight 107.90 Da
Charge 9.00
Isoelectric point 8.76
Molecular weight 36.69 kDa
Number of residues 340
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
855.06during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.6during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tfg3transcription factor TFIIF complex subunit Tfg3 Reconstituted ComplexPMID:15616156
External References
Database Identifier Description
NBRP SPAC16E8.16 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC16E8.16 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC16E8.16 BioGRID Interaction Datasets
Expression Viewer SPAC16E8.16 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC16E8.16 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC16E8.16 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC16E8.16 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC16E8.16 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC16E8.16 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC16E8.16 Cell Cycle Data
GEO SPAC16E8.16 GEO profiles
PInt SPAC16E8.16 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542339transcription factor TFIIB
EntrezGene2542339transcription factor TFIIB
SPD / RIKEN18/18D10Orfeome Localization Data
UniProtKB/SwissProtO13749Transcription initiation factor IIB
ModBaseO13749Database of comparative protein structure models
StringO13749Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594229transcription factor TFIIB
RefSeq mRNANM_001019652972h- transcription factor TFIIB (SPAC16E8.16), mRNA
European Nucleotide ArchiveCAB11044ENA Protein Mapping
UniParcUPI0000136C2FUniProt Archive

Literature for SPAC16E8.16

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014