ecm33 (SPAC1705.03c)

Gene Standard Nameecm33 Characterisation Statuspublished
Systematic IDSPAC1705.03c Feature Typeprotein coding
SynonymsSPAC1F2.01, SPAC23H4.19 Name Description
Productcell wall protein Ecm33 Product Size421aa, 43.35 kDa
Genomic Location Chromosome I, 1571567-1569613 (1955nt); CDS:1571260-1569995 (1266nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
fungal-type cell wall organization30
Annotation ExtensionEvidenceWith/FromReference
intracellular signal transduction223
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
anchored component of membrane33
Annotation ExtensionEvidenceWith/FromReference
cell surface92
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
external side of plasma membrane47
Annotation ExtensionEvidenceWith/FromReference
fungal-type cell wall34
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane967
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
decreased vegetative cell population growthecm33Δ588
sensitive to bleomycinecm33Δ77
sensitive to latrunculin Aecm33Δ24
sensitive to thiabendazoleecm33Δ235
viable vegetative cell populationecm33Δ3815

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologyecm33Δ3095
Target Of
FYPO affected by mutation in sak1 transcriptional repressor Sak1
GO regulated by atf1 transcription factor, Atf-CREB family Atf1
GO regulated by mbx1 MADS-box transcription factor Mbx1
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR1571567..1571261AU009794
3' UTR1569994..1569613AU010387
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PTHR31018 HMMPANTHER 26 402 2 Gene3D IPR000494 Receptor L-domain 44 116 2 Gene3D IPR000494 Receptor L-domain 23 43 2 Gene3D IPR000494 Receptor L-domain 117 189 2
SSF52058 SuperFamily 217 300 11
SSF52058 SuperFamily 23 124 11
SignalP-noTM signalp 1 19 212

View domain organization at Pfam

Term IDTerm NameReferenceCount

Protein Properties

Ave. residue weight 102.96 Da
Charge -24.50
Codon Adpatation Index 0.69
Isoelectric point 3.69
Molecular weight 43.35 kDa
Number of residues 421

Protein Modifications

Term NameResidueCount
glycosylphosphatidylinositolated residue 40
Annotation ExtensionEvidenceResidueReference
IDA PMID:17230583
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1242.68during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
97610during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
32676.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
48during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
5.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
promoter contains CRE element PMID:200323022
promoter contains RLM1 element PMID:200323021
Mkh1-Pek1-Spm1 MAP kinase PMID:200323023
Taxonomic Conservation
conserved in fungi only620
conserved in fungi4604
conserved in eukaryotes4514

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
binds DNA-binding domain construct withskp1SCF ubiquitin ligase complex subunit Skp1 Two-hybridPMID:17016471
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rescued by overexpression ofaah3alpha-amylase homolog Aah3 Dosage RescuePMID:22848669
overexpression rescuesapm1AP-1 adaptor complex mu subunit Apm1 Dosage RescuePMID:22848669
rescued by overexpression ofcis4cation diffusion family zinc transmembrane transporter Cis4 Dosage RescuePMID:22848669
overexpression rescuescis4cation diffusion family zinc transmembrane transporter Cis4 Dosage RescuePMID:22848669
synthetically rescued bypmk1MAP kinase Pmk1 Synthetic RescuePMID:20032302
rescued bypmk1MAP kinase Pmk1 Phenotypic SuppressionPMID:20032302
overexpression rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Dosage RescuePMID:20032302
rescued by overexpression ofSPBC1E8.05conserved fungal protein Dosage RescuePMID:22848669
rescued by overexpression ofubc4ubiquitin conjugating enzyme E2 Ubc4/UbcP1 Dosage RescuePMID:24454826
rescued by overexpression ofubi1ribosomal-ubiquitin fusion protein Ubi1 (predicted) Dosage RescuePMID:24454826
External References
Database Identifier Description
NBRP SPAC1705.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1705.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1705.03c BioGRID Interaction Datasets
Expression Viewer SPAC1705.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1705.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1705.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1705.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1705.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1705.03c Transcriptome Viewer (Bähler Lab)
GEO SPAC1705.03c GEO profiles
PInt SPAC1705.03c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1705.03c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1705.03c Fission yeast phenotypic data & analysis
Cyclebase SPAC1705.03c.1 Cell Cycle Data
SPD / RIKEN20/20C02Orfeome Localization Data
UniProtKB/SwissProtO13960Cell wall protein ecm33
ModBaseO13960Database of comparative protein structure models
STRINGO13960Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001713003972h- cell wall protein Ecm33 (ecm33), mRNA
RefSeq PeptideXP_001713055cell wall protein Ecm33
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveZ98976ENA EMBL mapping
European Nucleotide ArchiveCAB11655ENA Protein Mapping
European Nucleotide ArchiveCAB11655.3ENA Protein Mapping
European Nucleotide ArchiveCAB86946ENA Protein Mapping
European Nucleotide ArchiveCAB86946.1ENA Protein Mapping
UniParcUPI000006C8D2UniProt Archive

Literature for ecm33

Search: Europe PMC or PubMed

Release Version: PomBase:28_55 - 09 Sep 2015