ecm33 (SPAC1705.03c)


Gene Standard Nameecm33 Characterisation Statuspublished
Systematic IDSPAC1705.03c Feature Typeprotein coding
SynonymsSPAC1F2.01, SPAC23H4.19 Name Description
Productcell wall protein Ecm33 Product Size421aa, 43.35 kDa
Genomic Location Chromosome I, 1571567-1569613 (1955nt); CDS:1571260-1569995 (1266nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
calcium ion homeostasis16
Annotation ExtensionEvidenceWith/FromReference
fungal-type cell wall organization54
Annotation ExtensionEvidenceWith/FromReference
intracellular signal transduction265
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
anchored component of membrane33
Annotation ExtensionEvidenceWith/FromReference
cell surface92
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
external side of plasma membrane47
Annotation ExtensionEvidenceWith/FromReference
fungal-type cell wall34
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased vegetative cell population growthecm33ΔNull526
sensitive to bleomycinecm33ΔNull75
sensitive to latrunculin Aecm33ΔNull24
sensitive to thiabendazoleecm33ΔNull211
viable vegetative cell populationecm33ΔNull3795

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologyecm33ΔNull3093
Target Of
OntologyRelationshipGeneProductReference
GO regulated by atf1 transcription factor, Atf-CREB family Atf1 PMID:20032302
GO regulated by mbx1 MADS-box transcription factor Mbx1 PMID:20032302
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1571567..1569613
mRNA1571567..1569613
5' UTR1571567..1571261AU009794
CDS1571260..1569995
3' UTR1569994..1569613AU010387
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PTHR31018 HMMPANTHER 26 402 2
3.80.20.20 Gene3D IPR000494 Receptor L-domain 117 189 2
3.80.20.20 Gene3D IPR000494 Receptor L-domain 23 43 2
3.80.20.20 Gene3D IPR000494 Receptor L-domain 44 116 2
SSF52058 SuperFamily 23 124 11
SSF52058 SuperFamily 217 300 11
SignalP-noTM signalp 1 19 212

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279

Protein Properties

Ave. residue weight 102.96 Da
Charge -24.50
Isoelectric point 3.69
Molecular weight 43.35 kDa
Number of residues 421
Modifications

Protein Modifications

Term NameResidueCount
glycosylphosphatidylinositolated residue 40
Annotation ExtensionEvidenceResidueReference
IDA PMID:17230583
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1242.68during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
97610during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
32676.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
48during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
5.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
promoter contains CRE element PMID:200323022
promoter contains RLM1 element PMID:200323021
Pathway
DescriptionQualifierReferenceCount
Mkh1-Pek1-Spm1 MAP kinase PMID:200323023
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi only620
conserved in fungi4604
conserved in eukaryotes4516
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
binds DNA-binding domain construct withskp1SCF ubiquitin ligase complex subunit Skp1 Two-hybridPMID:17016471
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rescued by overexpression ofaah3alpha-amylase homolog Aah3 Dosage RescuePMID:22848669
overexpression rescuesapm1AP-1 adaptor complex mu subunit Apm1 Dosage RescuePMID:22848669
overexpression rescuescis4cation diffusion family zinc transmembrane transporter Cis4 Dosage RescuePMID:22848669
rescued by overexpression ofcis4cation diffusion family zinc transmembrane transporter Cis4 Dosage RescuePMID:22848669
synthetically rescued bypmk1MAP kinase Pmk1 Synthetic RescuePMID:20032302
rescued bypmk1MAP kinase Pmk1 Phenotypic SuppressionPMID:20032302
overexpression rescuesppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Dosage RescuePMID:20032302
rescued by overexpression ofSPBC1E8.05conserved fungal protein Dosage RescuePMID:22848669
rescued by overexpression ofubc4ubiquitin conjugating enzyme E2 Ubc4/UbcP1 Dosage RescuePMID:24454826
rescued by overexpression ofubi1ribosomal-ubiquitin fusion protein Ubi1 (predicted) Dosage RescuePMID:24454826
External References
Database Identifier Description
NBRP SPAC1705.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1705.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1705.03c BioGRID Interaction Datasets
Expression Viewer SPAC1705.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1705.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1705.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1705.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1705.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1705.03c Transcriptome Viewer (Bähler Lab)
GEO SPAC1705.03c GEO profiles
PInt SPAC1705.03c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1705.03c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1705.03c Fission yeast phenotypic data & analysis
Cyclebase SPAC1705.03c.1 Cell Cycle Data
SPD / RIKEN20/20C02Orfeome Localization Data
UniProtKB/SwissProtO13960Cell wall protein ecm33
ModBaseO13960Database of comparative protein structure models
STRINGO13960Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001713003972h- cell wall protein Ecm33 (ecm33), mRNA
RefSeq PeptideXP_001713055cell wall protein Ecm33
European Nucleotide ArchiveCAB11655.3ENA Protein Mapping
European Nucleotide ArchiveCAB86946.1ENA Protein Mapping
UniParcUPI000006C8D2UniProt Archive

Literature for ecm33

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015