saf3 (SPAC1782.03)


Gene Standard Namesaf3 Characterisation Statuspublished
Systematic IDSPAC1782.03 Feature Typeprotein coding
Synonyms Name Description
Productsplicing associated factor Saf3 Product Size355aa, 40.74 kDa
Genomic Location Chromosome I, 4761230-4763033 (1804nt); CDS:4761807-4762874 (1068nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
mRNA cis splicing, via spliceosome116
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
U2-type spliceosomal complex31
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopysaf3ΔNullPECO:0000005, PECO:0000137PMID:236978061338
Microscopysaf3ΔNullPMID:20473289
Cell growth assaysaf3ΔNullPMID:21386897

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001908increased pre-mRNA level23
affecting prp2Gel electrophoresis evidencesaf3ΔNullPMID:21386897
FYPO:0000311inviable after spore germination with normal, unseptated germ tube morphologyMicroscopysaf3ΔNullPECO:0000005, PECO:0000137PMID:23697806237
FYPO:0002423inviable after spore germination, without cell division, septated cells with abnormal cell shapeMicroscopysaf3ΔNullPMID:213868975
FYPO:0002151inviable spore465
penetrance FYPO_EXT:0000001Microscopysaf3ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
147612304763033

UTRs

Region Coordinates Reference
five_prime_UTR4761230..4761806PMID:21511999
three_prime_UTR4762875..4763033PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF06991 Pfam IPR009730 Micro-fibrillar-associated protein 1, C-terminal 95 353 1
PTHR15327:SF0 HMMPANTHER 18 354 1
PTHR15327 HMMPANTHER IPR009730 Micro-fibrillar-associated protein 1, C-terminal 18 354 1
Coil ncoils Rabaptin coiled-coil domain 199 242 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.76 Da
Charge -7.00
Isoelectric point 5.32
Molecular weight 40.74 kDa
Number of residues 355
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS347PMID:247631071670
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
208.17during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog602
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
snu66U4/U6 x U5 tri-snRNP complex subunit Snu66 Affinity Capture-MSPMID:21386897
smd1Sm snRNP core protein Smd1 Affinity Capture-MSPMID:21386897
prp45splicing factor Prp45 Affinity Capture-MSPMID:21386897
mug64BAR domain protein (predicted) Affinity Capture-MSPMID:21386897
cwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-MSPMID:21386897
lea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:21386897
cwf26complexed with Cdc5 protein Cwf26 Affinity Capture-MSPMID:21386897
spf38U5 snRNP complex subunit Spf38 Affinity Capture-MSPMID:21386897
cwf4complexed with Cdc5 protein Cwf4 Affinity Capture-MSPMID:21386897
dre4splicing associated factor Dre4 Affinity Capture-MSPMID:21386897
prp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Affinity Capture-MSPMID:21386897
sap61U2 snRNP-associated protein sap61 Affinity Capture-MSPMID:21386897
prp10U2 snRNP-associated protein Sap155 Affinity Capture-MSPMID:21386897
prp3U4/U6 x U5 tri-snRNP complex subunit Prp3 Affinity Capture-MSPMID:21386897
cwf3complexed with Cdc5 protein Cwf3 Affinity Capture-MSPMID:21386897
smd3Sm snRNP core protein Smd3 Affinity Capture-MSPMID:21386897
sap145U2 snRNP-associated protein Sap145 Affinity Capture-MSPMID:21386897
snu23U4/U6 x U5 tri-snRNP complex subunit Snu23 Affinity Capture-MSPMID:21386897
cwf29RNA-binding protein Cwf29 Affinity Capture-MSPMID:21386897
spp42U5 snRNP complex subunit Spp42 Affinity Capture-MSPMID:21386897
cwf5RNA-binding protein Cwf5 Affinity Capture-MSPMID:21386897
smb1Sm snRNP core protein Smb1 Affinity Capture-MSPMID:21386897
cwf7splicing factor Cwf7 Affinity Capture-MSPMID:21386897
rna4U4/U6 x U5 tri-snRNP complex subunit Prp4 family, Rna4 Affinity Capture-MSPMID:21386897
cdc5splicing factor, Prp19 complex subunit Cdc5 Affinity Capture-MSPMID:21386897
spf30splicing factor Spf30 (predicted) Affinity Capture-MSPMID:21386897
prp38U4/U6 x U5 tri-snRNP complex subunit Prp38 Affinity Capture-MSPMID:21386897
sap62U2 snRNP Sap62 Affinity Capture-MSPMID:21386897
prp19ubiquitin-protein ligase E4 Affinity Capture-MSPMID:21386897
prp12U2 snRNP-associated protein Sap130 Affinity Capture-MSPMID:21386897
prp5WD repeat protein Prp5 Affinity Capture-MSPMID:21386897
saf3splicing associated factor Saf3 Affinity Capture-MSPMID:21386897
rsd1RNA-binding protein Rsd1 (predicted) Affinity Capture-MSPMID:21386897
brr2U5 snRNP complex subunit Brr2 Affinity Capture-MSPMID:21386897
prp31U4/U6 x U5 tri-snRNP complex subunit Prp31 Affinity Capture-MSPMID:21386897
smg1Sm snRNP core protein Smg1 Affinity Capture-MSPMID:21386897
sap114U2 snRNP subunit Sap114 Affinity Capture-MSPMID:21386897
sap14U2 snRNP-associated protein SF3B14 Sap14 Affinity Capture-MSPMID:21386897
prp2U2AF large subunit (U2AF-59) Affinity Capture-MSPMID:21386897
saf2splicing associated factor Saf2 Affinity Capture-MSPMID:21386897
External References
Database Identifier Description
NBRP SPAC1782.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1782.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1782.03 BioGRID Interaction Datasets
Expression Viewer SPAC1782.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1782.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1782.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1782.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1782.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1782.03 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1782.03 Cell Cycle Data
GEO SPAC1782.03 GEO profiles
PInt SPAC1782.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1782.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1782.03 Fission yeast phenotypic data & analysis
SPD / RIKEN16/16D05Orfeome Localization Data
UniProtKB/SwissProtQ9P7H6Uncharacterized protein C1782.03
ModBaseQ9P7H6Database of comparative protein structure models
STRINGQ9P7H6Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594710splicing associated factor Saf3
RefSeq mRNANM_001020137972h- splicing associated factor Saf3 (saf3), mRNA
European Nucleotide ArchiveCAB76265.1ENA Protein Mapping
UniParcUPI000006ADAAUniProt Archive

Literature for saf3

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014