saf3 (SPAC1782.03)


Gene Standard Namesaf3 Characterisation Statuspublished
Systematic IDSPAC1782.03 Feature Typeprotein coding
Synonyms Name Description
Productsplicing associated factor Saf3 Product Size355aa, 40.74 kDa
Genomic Location Chromosome I, 4761230-4763033 (1804nt); CDS:4761807-4762874 (1068nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
mRNA cis splicing, via spliceosome117
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
U2-type spliceosomal complex48
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationsaf3ΔNull1435

Cell Phenotype

Term NameAlleleExpressionCount
increased pre-mRNA level28
affecting prp2saf3ΔNull
inviable after spore germination with normal, unseptated germ tube morphologysaf3ΔNull237
inviable after spore germination, without cell division, septated cells with abnormal cell shapesaf3ΔNull5
inviable sporesaf3ΔNull470
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4761230..4763033
mRNA4761230..4763033
5' UTR4761230..4761806PMID:21511999
CDS4761807..4762874
3' UTR4762875..4763033PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF06991 Pfam IPR009730 Micro-fibrillar-associated protein 1, C-terminal 95 353 1
PTHR15327 HMMPANTHER IPR009730 Micro-fibrillar-associated protein 1, C-terminal 85 355 1
PTHR15327:SF0 HMMPANTHER 85 355 1
Coil ncoils Rabaptin coiled-coil domain 199 242 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.76 Da
Charge -7.00
Isoelectric point 5.32
Molecular weight 40.74 kDa
Number of residues 355
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS347 1672
Annotation ExtensionEvidenceResidueReference
experimental evidence S347 PMID:24763107
phosphorylated residue 1926
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
208.17during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
no apparent S. cerevisiae ortholog598
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesbrr2U5 snRNP complex subunit Brr2 Affinity Capture-MSPMID:21386897
affinity capturescdc5splicing factor, Prp19 complex subunit Cdc5 Affinity Capture-MSPMID:21386897
affinity capturescwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-MSPMID:21386897
affinity capturescwf26complexed with Cdc5 protein Cwf26 Affinity Capture-MSPMID:21386897
affinity capturescwf29RNA-binding protein Cwf29 Affinity Capture-MSPMID:21386897
affinity capturescwf3complexed with Cdc5 protein Cwf3 Affinity Capture-MSPMID:21386897
affinity capturescwf4complexed with Cdc5 protein Cwf4 Affinity Capture-MSPMID:21386897
affinity capturescwf5RNA-binding protein Cwf5 Affinity Capture-MSPMID:21386897
affinity capturescwf7splicing factor Cwf7 Affinity Capture-MSPMID:21386897
affinity captured bydre4splicing associated factor Dre4 Affinity Capture-MSPMID:21386897
affinity capturesdre4splicing associated factor Dre4 Affinity Capture-MSPMID:21386897
affinity captureslea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:21386897
affinity capturesmug64BAR domain protein (predicted) Affinity Capture-MSPMID:21386897
affinity capturesprp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Affinity Capture-MSPMID:21386897
affinity capturesprp10U2 snRNP-associated protein Sap155 Affinity Capture-MSPMID:21386897
affinity capturesprp12U2 snRNP-associated protein Sap130 Affinity Capture-MSPMID:21386897
affinity capturesprp19ubiquitin-protein ligase E4 Prp19 Affinity Capture-MSPMID:21386897
affinity capturesprp2U2AF large subunit (U2AF-59) Affinity Capture-MSPMID:21386897
affinity capturesprp3U4/U6 x U5 tri-snRNP complex subunit Prp3 Affinity Capture-MSPMID:21386897
affinity capturesprp31U4/U6 x U5 tri-snRNP complex subunit Prp31 Affinity Capture-MSPMID:21386897
affinity capturesprp38U4/U6 x U5 tri-snRNP complex subunit Prp38 Affinity Capture-MSPMID:21386897
affinity capturesprp45splicing factor Prp45 Affinity Capture-MSPMID:21386897
affinity capturesprp5WD repeat protein Prp5 Affinity Capture-MSPMID:21386897
affinity capturesrna4U4/U6 x U5 tri-snRNP complex subunit Prp4 family, Rna4 Affinity Capture-MSPMID:21386897
affinity capturesrsd1RNA-binding protein Rsd1 (predicted) Affinity Capture-MSPMID:21386897
affinity capturessaf2splicing associated factor Saf2 Affinity Capture-MSPMID:21386897
affinity captured bysaf2splicing associated factor Saf2 Affinity Capture-MSPMID:21386897
affinity capturessaf3splicing associated factor Saf3 Affinity Capture-MSPMID:21386897
affinity capturessap114U2 snRNP subunit Sap114 Affinity Capture-MSPMID:21386897
affinity capturessap14U2 snRNP-associated protein SF3B14 Sap14 Affinity Capture-MSPMID:21386897
affinity capturessap145U2 snRNP-associated protein Sap145 Affinity Capture-MSPMID:21386897
affinity capturessap61U2 snRNP-associated protein sap61 Affinity Capture-MSPMID:21386897
affinity capturessap62U2 snRNP Sap62 Affinity Capture-MSPMID:21386897
affinity capturessmb1Sm snRNP core protein Smb1 Affinity Capture-MSPMID:21386897
affinity capturessmd1Sm snRNP core protein Smd1 Affinity Capture-MSPMID:21386897
affinity capturessmd3Sm snRNP core protein Smd3 Affinity Capture-MSPMID:21386897
affinity capturessmg1Sm snRNP core protein Smg1 Affinity Capture-MSPMID:21386897
affinity capturessnu23U4/U6 x U5 tri-snRNP complex subunit Snu23 Affinity Capture-MSPMID:21386897
affinity capturessnu66U4/U6 x U5 tri-snRNP complex subunit Snu66 Affinity Capture-MSPMID:21386897
affinity capturesspf30splicing factor Spf30 (predicted) Affinity Capture-MSPMID:21386897
affinity capturesspf38U5 snRNP complex subunit Spf38 Affinity Capture-MSPMID:21386897
affinity capturesspp42U5 snRNP complex subunit Spp42 Affinity Capture-MSPMID:21386897
External References
Database Identifier Description
NBRP SPAC1782.03 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1782.03 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1782.03 BioGRID Interaction Datasets
Expression Viewer SPAC1782.03 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1782.03 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1782.03 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1782.03 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1782.03 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1782.03 Transcriptome Viewer (Bähler Lab)
GEO SPAC1782.03 GEO profiles
PInt SPAC1782.03 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1782.03 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1782.03 Fission yeast phenotypic data & analysis
Cyclebase SPAC1782.03.1 Cell Cycle Data
SPD / RIKEN16/16D05Orfeome Localization Data
UniProtKB/SwissProtQ9P7H6Uncharacterized protein C1782.03
ModBaseQ9P7H6Database of comparative protein structure models
STRINGQ9P7H6Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594710splicing associated factor Saf3
RefSeq mRNANM_001020137972h- splicing associated factor Saf3 (saf3), mRNA
European Nucleotide ArchiveCAB76265.1ENA Protein Mapping
UniParcUPI000006ADAAUniProt Archive

Literature for saf3

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015