hrp1 (SPAC1783.05)


Gene Standard Namehrp1 Characterisation Statuspublished
Systematic IDSPAC1783.05 Feature Typeprotein coding
Synonymschd1 Name DescriptionHelicase-Related protein from S. Pombe
ProductATP-dependent DNA helicase Hrp1 Product Size1373aa, 158.55 kDa
Genomic Location Chromosome I, 2194820-2199471 (4652nt); CDS:2195017-2199138 (4122nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0004003ATP-dependent DNA helicase activityISMIPR002464GO_REF:000000143
GO:0003677DNA bindingIEAUniProtKB-KW:KW-0238GO_REF:0000037385
GO:0008094DNA-dependent ATPase activityIDAPMID:1075620366
GO:0005515protein bindingIPInap1PMID:17510629887
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032508DNA duplex unwindingISMIPR002464GO_REF:000000132
GO:0006338chromatin remodelingIMPPMID:10756203156
GO:0030702chromatin silencing at centromereIMPPMID:1590858653
GO:0030466chromatin silencing at silent mating-type cassetteIMPPMID:1590858632
GO:0048096chromatin-mediated maintenance of transcriptionTASPMID:95202662
GO:0007076mitotic chromosome condensationIMPPMID:107562039
GO:0035067negative regulation of histone acetylationIMPPMID:159085866
GO:0000122negative regulation of transcription from RNA polymerase II promoterIMPPMID:2062200874
GO:0045944positive regulation of transcription from RNA polymerase II promoterIMPPMID:2062200885
GO:0060303regulation of nucleosome densityIMPPMID:175106296
GO:0006369termination of RNA polymerase II transcriptionIMPPMID:1250401810
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000779condensed chromosome, centromeric regionIDAPMID:1590858676
GO:0016592mediator complexIPImed13PMID:2062200822
GO:0000790nuclear chromatinICGO:0004003GO_REF:0000001247
GO:0005634nucleusIDAPMID:107562032730
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyhrp1ΔNullPECO:0000005, PECO:0000137PMID:236978063755
Microscopyhrp1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidencehrp1ΔNullPECO:0000015PMID:23950735222
FYPO:0000143transcription regulation phenotypeChromatin immunoprecipitation experimenthrp1ΔNullPMID:2062200862
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopyhrp1ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
121948202199471

UTRs

Region Start End Reference
three_prime_UTR21991392199471PMID:21511999
five_prime_UTR21948202195016PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00385 Pfam IPR023780 Chromo domain/shadow 305 356 7
PF00385 Pfam IPR023780 Chromo domain/shadow 208 264 7
PF00176 Pfam IPR000330 SNF2-related 393 677 20
PF00271 Pfam IPR001650 Helicase, C-terminal 739 818 68
SM00487 SMART IPR014001 Helicase, superfamily 1/2, ATP-binding domain 386 580 70
SM00490 SMART IPR001650 Helicase, C-terminal 734 818 67
SM00298 SMART IPR000953 Chromo domain/shadow 206 269 9
SM00298 SMART IPR000953 Chromo domain/shadow 302 358 9
PS51192 Prosite Profiles IPR014001 Helicase, superfamily 1/2, ATP-binding domain 402 573 69
PS51194 Prosite Profiles IPR001650 Helicase, C-terminal 708 869 69
PS50013 Prosite Profiles IPR000953 Chromo domain/shadow 203 276 7
PS50013 Prosite Profiles IPR000953 Chromo domain/shadow 304 365 7
PTHR10799 HMMPANTHER 2 1244 21
PTHR10799:SF70 HMMPANTHER 2 1244 2
G3DSA:2.40.50.40 Gene3D 301 359 7
G3DSA:3.40.50.300 Gene3D 701 846 279
G3DSA:3.40.50.300 Gene3D 387 570 279
G3DSA:2.40.50.40 Gene3D 198 268 7
SSF52540 SuperFamily IPR027417 336 608 294
SSF54160 SuperFamily IPR016197 203 269 9
SSF52540 SuperFamily IPR027417 610 891 294
SSF54160 SuperFamily IPR016197 282 356 9
Coil ncoils Rabaptin coiled-coil domain 1314 1347 975
Coil ncoils Rabaptin coiled-coil domain 951 986 975
Low complexity (SEG) seg 1237 1255
Low complexity (SEG) seg 1262 1273
Low complexity (SEG) seg 51 66
Low complexity (SEG) seg 69 84
Low complexity (SEG) seg 126 141
Low complexity (SEG) seg 183 206
Low complexity (SEG) seg 948 960
Low complexity (SEG) seg 968 980

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000174DEAD/DEAH box helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000174
PBO:0000175helicase C-terminal domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000175
PBO:0001050chromodomain proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001050

Protein Properties

Ave. residue weight 115.48 Da
Charge -18.00
Isoelectric point 5.59
Molecular weight 158.55 kDa
Number of residues 1373
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residuePMID:182575171192
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2183.98during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
378.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
5.4during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
targets correlated with Clr3 and Sir2 targets PMID:175106292
overlapping genome wide occupancy in coding regions Nap1/Hrp1/Hrp3 PMID:175106293
overlapping genome wide occupancy in promoters Nap1/Hrp1/Hrp3 PMID:175106293
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
srb11cyclin CycC, Srb mediator subunit Srb11 Negative GeneticPMID:18818364
scs7sphingosine hydroxylase Scs7 Positive GeneticPMID:18818364
SPCC550.03cSki complex RNA helicase Ski2 (predicted) Negative GeneticPMID:18818364
hip1hira protein, histone chaperone Hip1 Negative GeneticPMID:18818364
cmk1calcium/calmodulin-dependent protein kinase Cmk1 Negative GeneticPMID:22681890
mug30ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
arp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
ago1argonaute Negative GeneticPMID:18818364
mug33Tea1-interacting protein involved in exocytosis Positive GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Positive GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
SPAC6F6.04cmembrane transporter (predicted) Negative GeneticPMID:22681890
mga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:18818364
mis6inner centromere protein, CENP-I ortholog Mis6 Synthetic Growth DefectPMID:15908586
atd1aldehyde dehydrogenase (predicted) Negative GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Positive GeneticPMID:18818364
cho1phosphatidyl-N-methylethanolamine N-methyltransferase (predicted) Negative GeneticPMID:18818364
clp1Cdc14-related protein phosphatase Clp1/Flp1 Negative GeneticPMID:22681890
SPCC16C4.20cHMG box protein (predicted) Negative GeneticPMID:22681890
ckb2CK2 family regulatory subunit (predicted) Negative GeneticPMID:18818364
set11ribosomal protein lysine methyltransferase Set11 Negative GeneticPMID:18818364
apq12nuclear membrane organization protein Apq12 (predicted) Negative GeneticPMID:22681890
fep1iron-sensing transcription factor Fep1 Positive GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Positive GeneticPMID:18818364
SPCC757.12alpha-amylase homolog (predicted) Negative GeneticPMID:18818364
ask1DASH complex subunit Ask1 Negative GeneticPMID:18818364
mgr2mitochondrial membrane protein Mgr2 (predicted) Negative GeneticPMID:22681890
ash2Ash2-trithorax family protein Negative GeneticPMID:18818364
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Positive GeneticPMID:18818364
dad2DASH complex subunit Dad2 Negative GeneticPMID:18818364
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
tma23ribosome biogenesis protein Tma23 (predicted) Negative GeneticPMID:18818364
mug154conserved fungal protein Negative GeneticPMID:18818364
SPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:18818364
SPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:18818364
mcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
SPBC29A3.09cAAA family ATPase Gcn20 (predicted) Negative GeneticPMID:18818364
amo1nuclear rim protein Amo1 Negative GeneticPMID:18818364
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:18818364
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:18818364
med13mediator complex subunit Srb9 Negative GeneticPMID:18818364
eri1double-strand siRNA ribonuclease Eri1 Negative GeneticPMID:22681890
ptc2protein phosphatase 2C Ptc2 Negative GeneticPMID:18818364
ppr2mitochondrial PPR repeat protein Ppr2 Negative GeneticPMID:22681890
csn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:18818364
hrp3ATP-dependent DNA helicase Hrp3 Synthetic LethalityPMID:15908586
Synthetic LethalityPMID:23103765
Phenotypic Enhancement
Phenotypic EnhancementPMID:23032292
cdm1DNA polymerase delta subunit Cdm1 Negative GeneticPMID:18818364
omh6alpha-1,2-mannosyltransferase Omh6 (predicted) Negative GeneticPMID:22681890
ubp3ubiquitin C-terminal hydrolase Ubp3 Positive GeneticPMID:18818364
SPCC584.15carrestin/PY protein 2 Positive GeneticPMID:22681890
spf1Set1C PHD Finger protein Spf1 Negative GeneticPMID:18818364
ser2phosphoserine phosphatase Ser2 (predicted) Negative GeneticPMID:18818364
SPCC1840.09NAD dependent epimerase/dehydratase family protein Negative GeneticPMID:22681890
dep1Sds3-like family protein Dep1 Negative GeneticPMID:22681890
set2histone lysine methyltransferase Set2 Positive GeneticPMID:22681890
mit1SHREC complex subunit Mit1 Synthetic LethalityPMID:23103765
raf1Rik1-associated factor Raf1 Positive GeneticPMID:22681890
fkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:18818364
SPAC1093.03inositol polyphosphate phosphatase (predicted) Positive GeneticPMID:18818364
rpl170260S ribosomal protein L17 (predicted) Negative GeneticPMID:22681890
alp14TOG ortholog Alp14 Negative GeneticPMID:18818364
elp2elongator complex subunit Elp2 (predicted) Negative GeneticPMID:18818364
get3GET complex ATPase subunit Get3 (predicted) Positive GeneticPMID:22681890
vps71Swr1 complex subunit Vps71 Negative GeneticPMID:18818364
nop52nucleolar protein Nop52 family (predicted) Negative GeneticPMID:22681890
vps901guanyl-nucleotide exchange factor Vps901 (predicted) Negative GeneticPMID:22681890
med15mediator complex subunit Med15 Synthetic LethalityPMID:20622008
bdf1Swr1 complex bromodomain subunit Brf1 Negative GeneticPMID:18818364
sdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:18818364
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
lsd2histone demethylase SWIRM2 (predicted) Affinity Capture-MSPMID:16990277
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
med13mediator complex subunit Srb9 Affinity Capture-WesternPMID:20622008
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
far8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
med15mediator complex subunit Med15 Affinity Capture-WesternPMID:20622008
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
External References
Database Identifier Description
NBRP SPAC1783.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1783.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1783.05 BioGRID Interaction Datasets
Expression Viewer SPAC1783.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1783.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1783.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1783.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1783.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1783.05 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1783.05 Cell Cycle Data
GEO SPAC1783.05 GEO profiles
PInt SPAC1783.05 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542363ATP-dependent DNA helicase Hrp1
EntrezGene2542363ATP-dependent DNA helicase Hrp1
SPD / RIKEN48/48G01Orfeome Localization Data
UniProtKB/SwissProtQ9US25Chromodomain helicase hrp1
ModBaseQ9US25Database of comparative protein structure models
StringQ9US25Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593660ATP-dependent DNA helicase Hrp1
RefSeq mRNANM_001019092972h- ATP-dependent DNA helicase Hrp1 (hrp1), mRNA
European Nucleotide ArchiveBAA87156ENA Protein Mapping
European Nucleotide ArchiveCAA67494ENA Protein Mapping
European Nucleotide ArchiveCAB66168ENA Protein Mapping
UniParcUPI000012CBC9UniProt Archive

Literature for hrp1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014