hrp1 (SPAC1783.05)


Gene Standard Namehrp1 Characterisation Statuspublished
Systematic IDSPAC1783.05 Feature Typeprotein coding
Synonymschd1 Name DescriptionHelicase-Related protein from S. Pombe
ProductATP-dependent DNA helicase Hrp1 Product Size1373aa, 158.55 kDa
Genomic Location Chromosome I, 2194820-2199471 (4652nt); CDS:2195017-2199138 (4122nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding557
Annotation ExtensionEvidenceWith/FromReference
DNA binding352
Annotation ExtensionEvidenceWith/FromReference
double-stranded DNA-dependent ATP-dependent DNA helicase activity1
Annotation ExtensionEvidenceWith/FromReference
protein binding887
Annotation ExtensionEvidenceWith/FromReference
single-stranded DNA-dependent ATP-dependent DNA helicase activity7
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ATP-dependent chromatin remodeling25
Annotation ExtensionEvidenceWith/FromReference
chromatin silencing at centromere58
Annotation ExtensionEvidenceWith/FromReference
chromatin silencing at centromere outer repeat region26
Annotation ExtensionEvidenceWith/FromReference
chromatin silencing at silent mating-type cassette36
Annotation ExtensionEvidenceWith/FromReference
chromatin-mediated maintenance of transcription2
Annotation ExtensionEvidenceWith/FromReference
negative regulation of histone acetylation3
Annotation ExtensionEvidenceWith/FromReference
negative regulation of transcription from RNA polymerase II promoter61
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter75
Annotation ExtensionEvidenceWith/FromReference
regulation of nucleosome density5
Annotation ExtensionEvidenceWith/FromReference
termination of RNA polymerase II transcription11
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
condensed nuclear chromosome, centromeric region70
Annotation ExtensionEvidenceWith/FromReference
mediator complex22
Annotation ExtensionEvidenceWith/FromReference
nuclear chromatin365
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencyhrp1Δ275
increased cell population growth ratehrp1Δ256
inviable vegetative cell populationhrp1+ (wild type)1455
normal growth on 6-azauracilhrp1Δ1
normal growth on glucose carbon sourcehrp1Δ294
sensitive to 5-fluorouracilhrp1Δ307
slow vegetative cell population growthhrp1+ (wild type)367
viable vegetative cell populationhrp1Δ3850

Cell Phenotype

Term NameGenotypesCount
abnormal nucleolar morphologyhrp1+ (wild type)4
abnormal regulation of transcription during vegetative growthhrp1Δ2
altered DNA level during vegetative growthhrp1+ (wild type)94
cuthrp1+ (wild type)128
decreased chromatin silencing at centromere outer repeathrp1Δ48
decreased mitotic chromosome condensationhrp1+ (wild type)12
increased antisense RNA levelhrp1Δ15
increased antisense RNA transcriptionhrp1Δ3
increased minichromosome loss during vegetative growthhrp1+ (wild type)76
hrp1Δ
lagging mitotic chromosomeshrp1Δ41
large and small daughter nucleihrp1+ (wild type)19
normal nucleosome positioning in euchromatinhrp1Δ6
viable vegetative cell with normal cell morphologyhrp1Δ3102

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
normal growth on glucose carbon sourcehrp1Δ, hrp3Δ

Cell Phenotype

Term NameGenotypes
abnormal nucleosome positioning in euchromatinhrp3Δ, hrp1Δ
decreased nucleosome occupancyhrp3Δ, hrp1Δ
increased antisense RNA levelhrp3Δ, hrp1Δ
normal histone H3-K9K14 acetylationhrp3Δ, hrp1Δ
normal level of histone H3 in cellhrp3Δ, hrp1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in abo1 ATPase with bromodomain protein
FYPO affected by mutation in hip1 hira protein, histone chaperone Hip1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2194820..2199471
mRNA2194820..2199471
5' UTR2194820..2195016PMID:21511999
CDS2195017..2199138
3' UTR2199139..2199471PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00385 Pfam IPR023780 Chromo domain 305 356 7
PF00385 Pfam IPR023780 Chromo domain 208 266 7
PF00176 Pfam IPR000330 SNF2-related, N-terminal domain 399 676 20
PF00271 Pfam IPR001650 Helicase, C-terminal 707 818 67
SM00490 SMART IPR001650 Helicase, C-terminal 734 818 68
SM00487 SMART IPR014001 Helicase superfamily 1/2, ATP-binding domain 386 580 69
SM00298 SMART IPR000953 Chromo/chromo shadow domain 302 358 9
SM00298 SMART IPR000953 Chromo/chromo shadow domain 206 269 9
PS50013 Prosite Profiles IPR000953 Chromo/chromo shadow domain 304 365 7
PS50013 Prosite Profiles IPR000953 Chromo/chromo shadow domain 203 276 7
PS51194 Prosite Profiles IPR001650 Helicase, C-terminal 708 869 69
PS51192 Prosite Profiles IPR014001 Helicase superfamily 1/2, ATP-binding domain 402 573 69
PTHR10799 HMMPANTHER 89 96 19
PTHR10799:SF70 HMMPANTHER 89 96 2
PTHR10799 HMMPANTHER 141 156 19
PTHR10799 HMMPANTHER 7 13 19
PTHR10799 HMMPANTHER 174 1053 19
PTHR10799:SF70 HMMPANTHER 174 1053 2
PTHR10799:SF70 HMMPANTHER 7 13 2
PTHR10799:SF70 HMMPANTHER 141 156 2
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 701 846 288
2.40.50.40 Gene3D C-C motif chemokine 15 198 268 7
2.40.50.40 Gene3D C-C motif chemokine 15 301 359 7
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 387 570 288
1.10.10.60 Gene3D IPR009057 Homeodomain-like 1131 1158 24
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 336 608 294
SSF54160 SuperFamily IPR016197 Chromo domain-like 203 269 9
SSF54160 SuperFamily IPR016197 Chromo domain-like 282 356 9
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 610 891 294
Coil ncoils Predicted coiled-coil protein (DUF2205) 951 985 1055
Coil ncoils Predicted coiled-coil protein (DUF2205) 1314 1346 1055

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000174DEAD/DEAH box helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000174
PBO:0000175helicase C-terminal domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000175
PBO:0001050chromodomain proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001050

Protein Properties

Ave. residue weight 115.48 Da
Charge -18.00
Codon Adaptation Index 0.44
Isoelectric point 5.59
Molecular weight 158.55 kDa
Number of residues 1373
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS93
present during mitotic M phaseS105
present during cellular response to thiabendazoleS1260
present during mitotic M phaseS145
S93, S116, S142, S145, S161, S163, S1260
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S93 PMID:21712547
present during mitotic M phase experimental evidence S105 PMID:21712547
present during cellular response to thiabendazole IDA S1260 PMID:18257517
present during mitotic M phase experimental evidence S145 PMID:21712547
IDA S1260 PMID:25720772
IDA S145 PMID:25720772
experimental evidence S145 PMID:24763107
IDA S163 PMID:25720772
IDA S93 PMID:25720772
IDA S142 PMID:25720772
IDA S161 PMID:25720772
IDA S116 PMID:25720772
O-phospho-L-threonineT118 1085
Annotation ExtensionEvidenceResidueReference
IDA T118 PMID:25720772
sumoylated lysine 174
Annotation ExtensionEvidenceResidueReference
IDA PMID:26404184
O-phosphorylated residueS116,Y117, S158,S161, T1259,S1260, T1259,S1260,T1264 2457
Annotation ExtensionEvidenceResidueReference
IDA S116,Y117 PMID:25720772
IDA T1259,S1260,T1264 PMID:25720772
IDA S158,S161 PMID:25720772
IDA T1259,S1260 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for hrp1 (SPAC1783.05)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
44321during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
46355during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
45884during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
43670during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
45792during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2183.98during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
378.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.4during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
targets correlated with Clr3 and Sir2 targets PMID:175106292
overlapping genome wide occupancy in coding regions Nap1/Hrp1/Hrp3 PMID:175106293
overlapping genome wide occupancy in promoters Nap1/Hrp1/Hrp3 PMID:175106293
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC1783.05 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bylsd2histone demethylase SWIRM2 (predicted) Affinity Capture-MSPMID:16990277
affinity captured bymed13mediator complex subunit Med13 Affinity Capture-WesternPMID:20622008
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured byfar8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
affinity captured bymed15mediator complex subunit Med15 Affinity Capture-WesternPMID:20622008
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC1783.05 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withmed15mediator complex subunit Med15 Synthetic LethalityPMID:20622008
synthetic lethal withmit1SHREC complex ATP-dependent DNA helicase subunit Mit1 Synthetic LethalityPMID:23103765
synthetic lethal withhrp3ATP-dependent DNA helicase Hrp3 Synthetic LethalityPMID:15908586
negative genetic interaction withfkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:18818364
negative genetic interaction withcdm1DNA polymerase delta subunit Cdm1 Negative GeneticPMID:18818364
negative genetic interaction withSPCC757.12alpha-amylase homolog (predicted) Negative GeneticPMID:18818364
negative genetic interaction withash2Ash2-trithorax family protein Negative GeneticPMID:18818364
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withmed13mediator complex subunit Med13 Negative GeneticPMID:18818364
negative genetic interaction withptc2protein phosphatase 2C Ptc2 Negative GeneticPMID:18818364
negative genetic interaction withmug154conserved fungal protein Negative GeneticPMID:18818364
negative genetic interaction withhap2HMG box protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withppr2mitochondrial PPR repeat protein Ppr2 Negative GeneticPMID:22681890
negative genetic interaction withapq12nuclear membrane organization protein Apq12 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:18818364
negative genetic interaction withclp1Cdc14-related protein phosphatase Clp1/Flp1 Negative GeneticPMID:22681890
negative genetic interaction withalp14TOG/XMAP14 family protein Alp14 Negative GeneticPMID:18818364
negative genetic interaction withmcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:22681890
negative genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Negative GeneticPMID:18818364
negative genetic interaction withdep1Sds3-like family protein Dep1 Negative GeneticPMID:22681890
negative genetic interaction withask1DASH complex subunit Ask1 Negative GeneticPMID:18818364
negative genetic interaction withski2Ski complex RNA helicase Ski2 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
negative genetic interaction withcoq11ubiquinone biosynthesis protein Coq11 Negative GeneticPMID:22681890
negative genetic interaction withelp2elongator complex subunit Elp2 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withSPAC6F6.04ctransmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withago1argonaute Negative GeneticPMID:18818364
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
negative genetic interaction withspf1Set1C PHD Finger protein Spf1 Negative GeneticPMID:18818364
negative genetic interaction withcho1phosphatidyl-N-dimethylethanolamine N-methyltransferase Negative GeneticPMID:18818364
negative genetic interaction withomh6alpha-1,2-mannosyltransferase Omh6 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhip1hira protein, histone chaperone Hip1 Negative GeneticPMID:18818364
negative genetic interaction withset11ribosomal protein lysine methyltransferase Set11 Negative GeneticPMID:18818364
negative genetic interaction withnop52nucleolar protein Nop52 family Rrp1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:18818364
negative genetic interaction withmgr2TIM23 translocase complex subunit Mgr2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withser2phosphoserine phosphatase Ser2 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withsdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:18818364
negative genetic interaction withckb2CK2 family regulatory subunit Ckb2 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withpuf1pumilio family RNA-binding protein Puf1 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withcmk1calcium/calmodulin-dependent protein kinase Cmk1 Negative GeneticPMID:22681890
negative genetic interaction witheri1double-strand siRNA ribonuclease Eri1 Negative GeneticPMID:22681890
negative genetic interaction withgcn20AAA family ATPase Gcn20 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withvps71Swr1 complex subunit Vps71 Negative GeneticPMID:18818364
negative genetic interaction withbdf1Swr1 complex bromodomain subunit Bdf1 Negative GeneticPMID:18818364
negative genetic interaction withatd1aldehyde dehydrogenase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrpl170260S ribosomal protein L17 (predicted) Negative GeneticPMID:22681890
negative genetic interaction witharp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
negative genetic interaction withmga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:18818364
negative genetic interaction withamo1nuclear rim protein Amo1 Negative GeneticPMID:18818364
negative genetic interaction withtma23ribosome biogenesis protein Tma23 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withcsn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:18818364
negative genetic interaction withvps901guanyl-nucleotide exchange factor Vps902 Negative GeneticPMID:22681890
positive genetic interaction withmug30HECT-type ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withubp3ubiquitin C-terminal hydrolase Ubp3 Positive GeneticPMID:18818364
positive genetic interaction withSPCC584.15carrestin involved in ubiquitin-dependent endocytosis Positive GeneticPMID:22681890
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withset2histone lysine methyltransferase Set2 Positive GeneticPMID:22681890
positive genetic interaction withscs7sphingosine hydroxylase Scs7 Positive GeneticPMID:18818364
positive genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Positive GeneticPMID:22681890
positive genetic interaction withmug33Tea1-interacting protein involved in exocytosis Positive GeneticPMID:22681890
positive genetic interaction withngg1SAGA complex subunit Ngg1/Ada3 Positive GeneticPMID:18818364
positive genetic interaction withraf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Positive GeneticPMID:22681890
positive genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Positive GeneticPMID:18818364
positive genetic interaction withfig4inositol polyphosphate phosphatase Fig4 (predicted) Positive GeneticPMID:18818364
positive genetic interaction withfep1iron-sensing transcription factor Fep1 Positive GeneticPMID:22681890
positive genetic interaction withget3GET complex ATPase subunit Get3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Positive GeneticPMID:18818364
phenotype enhanced byhrp3ATP-dependent DNA helicase Hrp3 Phenotypic EnhancementPMID:23032292
synthetic growth defect withmis6kinetochore protein, CENP-I ortholog Mis6 Synthetic Growth DefectPMID:15908586
External References
Database Identifier Description
NBRP SPAC1783.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1783.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1783.05 BioGRID Interaction Datasets
Expression Viewer SPAC1783.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1783.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1783.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1783.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1783.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1783.05 Transcriptome Viewer (Bähler Lab)
GEO SPAC1783.05 GEO profiles
PInt SPAC1783.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1783.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1783.05 Fission yeast phenotypic data & analysis
Cyclebase SPAC1783.05.1 Cell Cycle Data
SPD / RIKEN48/48G01Orfeome Localization Data
UniProtKB/SwissProtQ9US25Chromodomain helicase hrp1
ModBaseQ9US25Database of comparative protein structure models
STRINGQ9US25Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593660ATP-dependent DNA helicase Hrp1
RefSeq mRNANM_001019092972h- ATP-dependent DNA helicase Hrp1 (hrp1), mRNA
European Nucleotide ArchiveBAA87156.1ENA Protein Mapping
European Nucleotide ArchiveCAA67494.1ENA Protein Mapping
European Nucleotide ArchiveCAB66168.1ENA Protein Mapping
UniParcUPI000012CBC9UniProt Archive

Literature for hrp1

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016