hrp1 (SPAC1783.05)

Gene Standard Namehrp1 Characterisation Statuspublished
Systematic IDSPAC1783.05 Feature Typeprotein coding
Synonymschd1 Name DescriptionHelicase-Related protein from S. Pombe
ProductATP-dependent DNA helicase Hrp1 Product Size1373aa, 158.55 kDa
Genomic Location Chromosome I, 2194820-2199471 (4652nt); CDS:2195017-2199138 (4122nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
ATP-dependent DNA helicase activity42
Annotation ExtensionEvidenceWith/FromReference
DNA binding383
Annotation ExtensionEvidenceWith/FromReference
DNA-dependent ATPase activity65
Annotation ExtensionEvidenceWith/FromReference
protein binding831
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
chromatin remodeling160
Annotation ExtensionEvidenceWith/FromReference
chromatin silencing at centromere56
Annotation ExtensionEvidenceWith/FromReference
chromatin silencing at silent mating-type cassette33
Annotation ExtensionEvidenceWith/FromReference
chromatin-mediated maintenance of transcription2
Annotation ExtensionEvidenceWith/FromReference
DNA duplex unwinding31
Annotation ExtensionEvidenceWith/FromReference
mitotic chromosome condensation10
Annotation ExtensionEvidenceWith/FromReference
negative regulation of histone acetylation3
Annotation ExtensionEvidenceWith/FromReference
negative regulation of transcription from RNA polymerase II promoter79
Annotation ExtensionEvidenceWith/FromReference
positive regulation of transcription from RNA polymerase II promoter87
Annotation ExtensionEvidenceWith/FromReference
regulation of nucleosome density5
Annotation ExtensionEvidenceWith/FromReference
termination of RNA polymerase II transcription10
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
condensed nuclear chromosome, centromeric region66
Annotation ExtensionEvidenceWith/FromReference
mediator complex22
Annotation ExtensionEvidenceWith/FromReference
nuclear chromatin283
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000636increased cell population growth rateCell growth assayhrp1ΔNullPMID:952026610
FYPO:0001234slow vegetative cell population growthCell growth assayhrp1+ (wild type)OverexpressionPMID:952026678
FYPO:0002060viable vegetative cell populationMicroscopyhrp1ΔNullPECO:0000005, PECO:0000137PMID:236978063759

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidencehrp1ΔNullPECO:0000015PMID:23950735240
FYPO:0002913increased antisense RNA transcriptionTranscript expression level evidencehrp1ΔNullPMID:251227514
FYPO:0000143transcription regulation phenotypeChromatin immunoprecipitation experimenthrp1ΔNullPMID:2062200888
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyhrp1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00385 Pfam IPR023780 Chromo domain 305 356 7
PF00385 Pfam IPR023780 Chromo domain 208 264 7
PF00271 Pfam IPR001650 Helicase, C-terminal 739 818 68
PF00176 Pfam IPR000330 SNF2-related 393 677 20
SM00490 SMART IPR001650 Helicase, C-terminal 734 818 68
SM00298 SMART IPR000953 Chromo domain/shadow 206 269 9
SM00298 SMART IPR000953 Chromo domain/shadow 302 358 9
SM00487 SMART IPR014001 Helicase, superfamily 1/2, ATP-binding domain 386 580 69
PS50013 Prosite Profiles IPR000953 Chromo domain/shadow 203 276 7
PS50013 Prosite Profiles IPR000953 Chromo domain/shadow 304 365 7
PS51194 Prosite Profiles IPR001650 Helicase, C-terminal 708 869 69
PS51192 Prosite Profiles IPR014001 Helicase, superfamily 1/2, ATP-binding domain 402 573 69
PTHR10799:SF70 HMMPANTHER 2 1244 2
PTHR10799 HMMPANTHER 2 1244 21 Gene3D 701 846 288 Gene3D IPR009057 Homeodomain-like 1131 1158 24 Gene3D 301 359 7 Gene3D 198 268 7 Gene3D 387 570 288
SSF54160 SuperFamily IPR016197 Chromo domain-like 282 356 9
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 336 608 294
SSF54160 SuperFamily IPR016197 Chromo domain-like 203 269 9
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 610 891 294
Coil ncoils Rabaptin coiled-coil domain 951 986 968
Coil ncoils Rabaptin coiled-coil domain 1314 1347 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000174DEAD/DEAH box helicaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000174
PBO:0000175helicase C-terminal domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000175
PBO:0001050chromodomain proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001050

Protein Properties

Ave. residue weight 115.48 Da
Charge -18.00
Isoelectric point 5.59
Molecular weight 158.55 kDa
Number of residues 1373

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS145PMID:247631071670
present during mitotic M phaseexperimental evidenceS105PMID:21712547
present during mitotic M phaseexperimental evidenceS93PMID:21712547
present during mitotic M phaseexperimental evidenceS145PMID:21712547
present during cellular response to thiabendazoleIDAS1260PMID:18257517
MOD:00696phosphorylated residueNASPMID:182575171922
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
44321during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
46355during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
45884during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
43670during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
45792during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
2183.98during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
378.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.4during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
1.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
targets correlated with Clr3 and Sir2 targets PMID:175106292
overlapping genome wide occupancy in coding regions Nap1/Hrp1/Hrp3 PMID:175106293
overlapping genome wide occupancy in promoters Nap1/Hrp1/Hrp3 PMID:175106293
Species Distribution
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
lsd2histone demethylase SWIRM2 (predicted) Affinity Capture-MSPMID:16990277
med13mediator complex subunit Srb9 Affinity Capture-WesternPMID:20622008
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
far8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
med15mediator complex subunit Med15 Affinity Capture-WesternPMID:20622008
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
srb11cyclin CycC, Srb mediator subunit Srb11 Negative GeneticPMID:18818364
scs7sphingosine hydroxylase Scs7 Positive GeneticPMID:18818364
SPCC550.03cSki complex RNA helicase Ski2 (predicted) Negative GeneticPMID:18818364
hip1hira protein, histone chaperone Hip1 Negative GeneticPMID:18818364
cmk1calcium/calmodulin-dependent protein kinase Cmk1 Negative GeneticPMID:22681890
mug30HECT-type ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
arp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:22681890
ago1argonaute Negative GeneticPMID:18818364
mug33Tea1-interacting protein involved in exocytosis Positive GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Positive GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
SPAC6F6.04cmembrane transporter (predicted) Negative GeneticPMID:22681890
mga2IPT/TIG ankyrin repeat containing transcription regulator of fatty acid biosynthesis (predicted) Negative GeneticPMID:18818364
mis6inner centromere protein, CENP-I ortholog Mis6 Synthetic Growth DefectPMID:15908586
atd1aldehyde dehydrogenase (predicted) Negative GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Positive GeneticPMID:18818364
cho1phosphatidyl-N-methylethanolamine N-methyltransferase (predicted) Negative GeneticPMID:18818364
clp1Cdc14-related protein phosphatase Clp1/Flp1 Negative GeneticPMID:22681890
SPCC16C4.20cHMG box protein (predicted) Negative GeneticPMID:22681890
ckb2CK2 family regulatory subunit Ckb2 (predicted) Negative GeneticPMID:18818364
set11ribosomal protein lysine methyltransferase Set11 Negative GeneticPMID:18818364
apq12nuclear membrane organization protein Apq12 (predicted) Negative GeneticPMID:22681890
fep1iron-sensing transcription factor Fep1 Positive GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Positive GeneticPMID:18818364
SPCC757.12alpha-amylase homolog (predicted) Negative GeneticPMID:18818364
ask1DASH complex subunit Ask1 Negative GeneticPMID:18818364
mgr2mitochondrial membrane protein Mgr2 (predicted) Negative GeneticPMID:22681890
ash2Ash2-trithorax family protein Negative GeneticPMID:18818364
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Positive GeneticPMID:18818364
dad2DASH complex subunit Dad2 Negative GeneticPMID:18818364
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
tma23ribosome biogenesis protein Tma23 (predicted) Negative GeneticPMID:18818364
mug154conserved fungal protein Negative GeneticPMID:18818364
SPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:18818364
SPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:18818364
mcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
SPBC29A3.09cAAA family ATPase Gcn20 (predicted) Negative GeneticPMID:18818364
amo1nuclear rim protein Amo1 Negative GeneticPMID:18818364
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:18818364
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:18818364
med13mediator complex subunit Srb9 Negative GeneticPMID:18818364
eri1double-strand siRNA ribonuclease Eri1 Negative GeneticPMID:22681890
ptc2protein phosphatase 2C Ptc2 Negative GeneticPMID:18818364
ppr2mitochondrial PPR repeat protein Ppr2 Negative GeneticPMID:22681890
csn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:18818364
hrp3ATP-dependent DNA helicase Hrp3 Synthetic LethalityPMID:15908586
Phenotypic EnhancementPMID:23103765
Synthetic Lethality
Phenotypic EnhancementPMID:23032292
cdm1DNA polymerase delta subunit Cdm1 Negative GeneticPMID:18818364
omh6alpha-1,2-mannosyltransferase Omh6 (predicted) Negative GeneticPMID:22681890
ubp3ubiquitin C-terminal hydrolase Ubp3 Positive GeneticPMID:18818364
SPCC584.15carrestin/PY protein 2 Positive GeneticPMID:22681890
spf1Set1C PHD Finger protein Spf1 Negative GeneticPMID:18818364
ser2phosphoserine phosphatase Ser2 (predicted) Negative GeneticPMID:18818364
SPCC1840.09NAD dependent epimerase/dehydratase family protein Negative GeneticPMID:22681890
dep1Sds3-like family protein Dep1 Negative GeneticPMID:22681890
set2histone lysine methyltransferase Set2 Positive GeneticPMID:22681890
mit1SHREC complex subunit Mit1 Synthetic LethalityPMID:23103765
raf1CLRC ubiquitin E3 ligase complex specificiy factor Raf1/Dos1 Positive GeneticPMID:22681890
fkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:18818364
SPAC1093.03inositol polyphosphate phosphatase (predicted) Positive GeneticPMID:18818364
rpl170260S ribosomal protein L17 (predicted) Negative GeneticPMID:22681890
alp14TOG ortholog Alp14 Negative GeneticPMID:18818364
elp2elongator complex subunit Elp2 (predicted) Negative GeneticPMID:18818364
get3GET complex ATPase subunit Get3 (predicted) Positive GeneticPMID:22681890
vps71Swr1 complex subunit Vps71 Negative GeneticPMID:18818364
nop52nucleolar protein Nop52 family (predicted) Negative GeneticPMID:22681890
vps901guanyl-nucleotide exchange factor Vps901 (predicted) Negative GeneticPMID:22681890
med15mediator complex subunit Med15 Synthetic LethalityPMID:20622008
bdf1Swr1 complex bromodomain subunit Brf1 Negative GeneticPMID:18818364
sdh4TIM22 inner membrane protein import complex anchor subunit Tim18 Negative GeneticPMID:18818364
External References
Database Identifier Description
NBRP SPAC1783.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1783.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1783.05 BioGRID Interaction Datasets
Expression Viewer SPAC1783.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1783.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1783.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1783.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1783.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1783.05 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1783.05 Cell Cycle Data
GEO SPAC1783.05 GEO profiles
PInt SPAC1783.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1783.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1783.05 Fission yeast phenotypic data & analysis
SPD / RIKEN48/48G01Orfeome Localization Data
UniProtKB/SwissProtQ9US25Chromodomain helicase hrp1
ModBaseQ9US25Database of comparative protein structure models
STRINGQ9US25Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593660ATP-dependent DNA helicase Hrp1
RefSeq mRNANM_001019092972h- ATP-dependent DNA helicase Hrp1 (hrp1), mRNA
European Nucleotide ArchiveBAA87156.1ENA Protein Mapping
European Nucleotide ArchiveCAA67494.1ENA Protein Mapping
European Nucleotide ArchiveCAB66168.1ENA Protein Mapping
UniParcUPI000012CBC9UniProt Archive

Literature for hrp1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014