bsu1 (SPAC17A2.01)

Gene Standard Namebsu1 ChromosomeI
Systematic IDSPAC17A2.01 Gene Start3549682
Synonymscar1, sod1, SPAC1B1.05 Gene End3552067
Producthigh-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1 Gene Length2386
Feature Typeprotein coding CDS Start3550152
Name Descriptionvitamin B Six Uptake,
Changed Amiloride Resistance		 CDS End3551732
Characterisation Statuspublished CDS Length1581

Ensembl Gene Location
  1. kap95
  2. SPNCRNA.915
  3. SPAC1B1.04c
  4. SPRRNA.15
  5. bsu1
  6. SPAC17A2.02c
  7. SPNCRNA.916
  8. vma6
  9. cns1
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000047normal cell population growthCell growth assaycar1-5 (unknown)unknownPECO:0000014, PECO:0000108PMID:8675019
Cell growth assaybsu1delta (deletion)deletionPECO:0000014, PECO:0000107PMID:8675019
Cell growth assaycar1-2 (unknown)unknownPECO:0000014, PECO:0000106PMID:8675019
Cell growth assaybsu1delta (deletion)deletionPECO:0000014, PECO:0000106PMID:8675019
Cell growth assaycar1-2 (unknown)unknownPECO:0000014, PECO:0000107PMID:8675019
Cell growth assaybsu1delta (deletion)deletionPECO:0000014, PECO:0000108PMID:8675019
Cell growth assaycar1-2 (unknown)unknownPECO:0000014, PECO:0000108PMID:8675019
Cell growth assaycar1-5 (unknown)unknownPECO:0000014, PECO:0000107PMID:8675019
Cell growth assaycar1-5 (unknown)unknownPECO:0000014, PECO:0000106PMID:8675019
FYPO:0000125resistance to amilorideCell growth assaybsu1delta (deletion)deletionPMID:8675019
bsu1delta (deletion)deletionPMID:8246883
Cell growth assaycar1-5 (unknown)unknownPMID:8675019
Cell growth assaycar1-2 (unknown)unknownPMID:8675019
FYPO:0002060viable vegetative cell populationMicroscopybsu1delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0015234thiamine transmembrane transporter activityIGIthi9PMID:18201975
GO:0031925pyridoxal transmembrane transporter activityIDAPMID:15701794
GO:0031927pyridoxamine transmembrane transporter activityIDAPMID:15701794
GO:0031928pyridoxine transmembrane transporter activityIDAPMID:15701794
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0031920pyridoxal transportIDAPMID:15701794
GO:0031922pyridoxamine transportIDAPMID:15701794
GO:0031923pyridoxine transportIDAPMID:15701794
GO:0036225cellular response to vitamin B1 starvationIMPPMID:8675019
GO:0071934thiamine transmembrane transport
redundant_withIGIthi9PMID:18201975
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0005887integral to plasma membraneNASPMID:15701794
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
135496823552067

References

Region Start End Reference
three_prime_UTR35517333552067PMID:21511999
three_prime_UTR35517333552067PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF07690 IPR011701 Pfam Major facilitator superfamily 87 464 48
TMhelix TMHMM 81 103 955
TMhelix TMHMM 210 232 955
TMhelix TMHMM 443 465 955
TMhelix TMHMM 385 407 955
TMhelix TMHMM 173 195 955
TMhelix TMHMM 239 261 955
TMhelix TMHMM 148 166 955
TMhelix TMHMM 116 138 955
TMhelix TMHMM 308 330 955
TMhelix TMHMM 480 502 955
TMhelix TMHMM 343 365 955
PS50850 IPR020846 Prosite Profiles Major facilitator superfamily domain 83 506 53
G3DSA:1.20.1250.20 Gene3D 59 262 67
SSF103473 IPR016196 SuperFamily 66 500 74
PTHR24003 hmmpanther 12 510 28
PTHR24003:SF225 hmmpanther 12 510 3

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReference
SO:0001812transmembrane_helix

Protein Properties

Ave. residue weight 111.19 Da
Charge 4.50
Isoelectric point 7.47
Molecular weight 58.48 kDa
Number of residues 526
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide8505.91PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127		population_widePMID:23101633
transcriptionWestern blot evidencedecreasedPMID:8675019
RNA levelNorthern assay evidencedecreasedPMID:8675019
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide23PMID:23101633
Northern assay evidencepresentPMID:8675019
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide1.8PMID:23101633
Northern assay evidencecellular response to vitamin B1decreasedPMID:8675019
Northern assay evidencepresentPMID:8675019
Warning
DescriptionQualifierReference
gene name conflict
S. cerevisiae PN uptake is mediated by the unrelated transporter Tpn1p
Species Distribution
DescriptionQualifierReference
orthologs cannot be distinguished
conserved in fungi
conserved in eukaryotes
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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
thi9thiamine transporter Thi9 Synthetic Growth DefectPMID:18201975
nmt14-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase Nmt1 Synthetic Growth DefectPMID:8675019
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External References
Database Identifier Description
NBRP SPAC17A2.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17A2.01 Retrieval of eukaryotic orthologs
BioGrid SPAC17A2.01 BioGRID Interaction Datasets
Bähler Lab SPAC17A2.01 Cell Cycle Expression Profile
Bähler Lab SPAC17A2.01 Meiosis/Sporulation Expression Profies
Bähler Lab SPAC17A2.01 Pheromone response/mating expression profiles
Bähler Lab SPAC17A2.01 Environmental stress expression profiles
Bähler Lab SPAC17A2.01 Bähler Lab Transcriptome Viewer
Cyclebase SPAC17A2.01 Cell Cycle Data
PInt SPAC17A2.01 Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC17A2.01 Entrez Gene
WikiGene3361460high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1
Uniprot_genenameBSU1
EntrezGene3361460high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1
SPD / RIKEN45/45C01Orfeome Localization Data
UniProtKB/SwissProtP33532Vitamin B6 transporter bsu1
ModBaseP33532Database of comparative protein structure models
Pfam Protein DomainsP33532Pfam Domain Arrangement
RefSeq_mRNA_predictedXM_001713032972h- high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1 (bsu1), mRNA
RefSeq_peptide_predictedXP_001713084high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1
European Nucleotide ArchiveCAA78411ENA Protein Mapping
European Nucleotide ArchiveCAB11084ENA Protein Mapping
UniParcUPI0000126F1EUniProt Archive

Literature for bsu1
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013