bsu1 (SPAC17A2.01)


Gene Standard Namebsu1 Characterisation Statuspublished
Systematic IDSPAC17A2.01 Feature Typeprotein coding
Synonymscar1, sod1, SPAC1B1.05 Name Descriptionvitamin B Six Uptake,
Changed Amiloride Resistance
Producthigh-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1 Product Size526aa, 58.48 kDa
Genomic Location Chromosome I, 3549682-3552067 (2386nt); CDS:3550152-3551732 (1581nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
pyridoxal transmembrane transporter activity3
Annotation ExtensionEvidenceWith/FromReference
pyridoxamine transmembrane transporter activity3
Annotation ExtensionEvidenceWith/FromReference
pyridoxine transmembrane transporter activity3
Annotation ExtensionEvidenceWith/FromReference
thiamine transmembrane transporter activity3
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
pyridoxal transmembrane transport3
Annotation ExtensionEvidenceWith/FromReference
pyridoxamine transmembrane transport3
Annotation ExtensionEvidenceWith/FromReference
pyridoxine transmembrane transport3
Annotation ExtensionEvidenceWith/FromReference
thiamine transmembrane transport3
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
plasma membrane249
Annotation ExtensionEvidenceWith/FromReference
vacuole185
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
increased cell population growth ratebsu1ΔNull247
normal growth on glucose carbon sourcebsu1ΔNull207
normal vegetative cell population growthbsu1ΔNull738
car1-2
car1-5
resistance to amiloridebsu1ΔNull2
car1-2
car1-5
viable vegetative cell populationbsu1ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
decreased pyridoxine importbsu1ΔNull1
normal sodium importbsu1ΔNull1
viable vegetative cell with normal cell morphologybsu1ΔNull3094
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in snz1 pyridoxine biosynthesis protein
FYPO affected by mutation in tnr3 thiamine diphosphokinase Tnr3/ Nudix hydrolase fusion protein
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3549682..3552067
mRNA3549682..3552067
5' UTR3549682..3550151AU007811
CDS3550152..3551732
3' UTR3551733..3552067PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07690 Pfam IPR011701 Major facilitator superfamily 87 463 50
TMhelix TMHMM 343 365 959
TMhelix TMHMM 81 103 959
TMhelix TMHMM 210 232 959
TMhelix TMHMM 480 502 959
TMhelix TMHMM 385 407 959
TMhelix TMHMM 148 166 959
TMhelix TMHMM 239 261 959
TMhelix TMHMM 173 195 959
TMhelix TMHMM 308 330 959
TMhelix TMHMM 443 465 959
TMhelix TMHMM 116 138 959
PS50850 Prosite Profiles IPR020846 Major facilitator superfamily domain 83 506 55
PTHR24004 HMMPANTHER 11 516 21
PTHR24004:SF68 HMMPANTHER 11 516 3
1.20.1250.20 Gene3D 59 262 72
1.20.1250.20 Gene3D 307 499 72
SSF103473 SuperFamily IPR020846 Major facilitator superfamily domain 66 500 76

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 111.19 Da
Charge 4.50
Isoelectric point 7.47
Molecular weight 58.48 kDa
Number of residues 526
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS34
present during mitotic M phaseS16
level fluctuates during mitotic cell cycleS7
S12, S16, S44
present during mitotic M phaseS33
present during mitotic M phaseS7
present during mitotic M phaseS36
present during mitotic M phaseS12
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S7 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S7 PMID:24763107
experimental evidence S12 PMID:24763107
present during mitotic M phase experimental evidence S12 PMID:21712547
experimental evidence S16 PMID:24763107
present during mitotic M phase experimental evidence S16 PMID:21712547
present during mitotic M phase experimental evidence S33 PMID:21712547
present during mitotic M phase experimental evidence S34 PMID:21712547
present during mitotic M phase experimental evidence S36 PMID:21712547
experimental evidence S44 PMID:24763107
O-phospho-L-threonine 698
present during mitotic M phaseT14
present during mitotic M phaseT31
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T14 PMID:21712547
present during mitotic M phase experimental evidence T31 PMID:21712547
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelpresent during GO:0036225Western blot evidencePMID:15701794
absent during GO:0071301Western blot evidencePMID:15701794
transcriptiondecreased in presence of thiamine Western blot evidencePMID:8675019
RNA leveldecreased during GO:0071301Northern assay evidencePMID:8675019
present during GO:0036225Northern assay evidencePMID:15701794
decreased in presence of aminopyrimidine Northern assay evidencePMID:8675019
absent during GO:0071301Northern assay evidencePMID:15701794
present in presence of amiloride Northern assay evidencePMID:8675019
present in presence of thiazole Northern assay evidencePMID:8675019

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4760during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6419during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5481during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5613during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
8505.91during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
5840during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
23during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene name conflict19
S. cerevisiae PN uptake is mediated by the unrelated transporter Tpn1p1
Species Distribution
DescriptionQualifierReferenceCount
orthologs cannot be distinguished129
conserved in fungi4603
conserved in eukaryotes4514
Orthologs

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withnmt14-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase Nmt1 Synthetic Growth DefectPMID:8675019
synthetic growth defect withthi9thiamine transmembrane transporter Thi9 Synthetic Growth DefectPMID:18201975
External References
Database Identifier Description
NBRP SPAC17A2.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17A2.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17A2.01 BioGRID Interaction Datasets
Expression Viewer SPAC17A2.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17A2.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17A2.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17A2.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17A2.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17A2.01 Transcriptome Viewer (Bähler Lab)
GEO SPAC17A2.01 GEO profiles
PInt SPAC17A2.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC17A2.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC17A2.01 Fission yeast phenotypic data & analysis
Cyclebase SPAC17A2.01.1 Cell Cycle Data
SPD / RIKEN45/45C01Orfeome Localization Data
UniProtKB/SwissProtP33532Vitamin B6 transporter bsu1
ModBaseP33532Database of comparative protein structure models
STRINGP33532Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001713032972h- high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1 (bsu1), mRNA
RefSeq PeptideXP_001713084high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1
European Nucleotide ArchiveCAA78411.1ENA Protein Mapping
European Nucleotide ArchiveCAB11084.1ENA Protein Mapping
UniParcUPI0000126F1EUniProt Archive

Literature for bsu1

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015