bsu1 (SPAC17A2.01)


Gene Standard Namebsu1 Characterisation Statuspublished
Systematic IDSPAC17A2.01 Feature Typeprotein coding
Synonymscar1, sod1, SPAC1B1.05 Name Descriptionvitamin B Six Uptake,
Changed Amiloride Resistance
Producthigh-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1 Product Size526aa, 58.48 kDa
Genomic Location Chromosome I, 3549682-3552067 (2386nt); CDS:3550152-3551732 (1581nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
pyridoxal transmembrane transporter activity3
Annotation ExtensionEvidenceWith/FromReference
pyridoxamine transmembrane transporter activity3
Annotation ExtensionEvidenceWith/FromReference
pyridoxine transmembrane transporter activity3
Annotation ExtensionEvidenceWith/FromReference
thiamine transmembrane transporter activity3
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
pyridoxal transmembrane transport3
Annotation ExtensionEvidenceWith/FromReference
pyridoxamine transmembrane transport3
Annotation ExtensionEvidenceWith/FromReference
pyridoxine transmembrane transport3
Annotation ExtensionEvidenceWith/FromReference
thiamine transmembrane transport3
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
integral component of membrane966
Annotation ExtensionEvidenceWith/FromReference
plasma membrane253
Annotation ExtensionEvidenceWith/FromReference
vacuole240
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
increased cell population growth ratebsu1Δ256
normal growth on glucose carbon sourcebsu1Δ294
normal vegetative cell population growthcar1-2813
bsu1Δ
car1-5
resistance to amiloridecar1-22
car1-5
bsu1Δ
viable vegetative cell populationbsu1Δ3850

Cell Phenotype

Term NameGenotypesCount
decreased pyridoxine importbsu1Δ1
normal sodium importbsu1Δ1
viable vegetative cell with normal cell morphologybsu1Δ3102
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3549682..3552067
mRNA3549682..3552067
5' UTR3549682..3550151AU007811
CDS3550152..3551732
3' UTR3551733..3552067PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07690 Pfam IPR011701 Major facilitator superfamily 87 464 50
TMhelix TMHMM 148 166 950
TMhelix TMHMM 239 261 950
TMhelix TMHMM 173 195 950
TMhelix TMHMM 308 330 950
TMhelix TMHMM 81 103 950
TMhelix TMHMM 443 465 950
TMhelix TMHMM 385 407 950
TMhelix TMHMM 210 232 950
TMhelix TMHMM 343 365 950
TMhelix TMHMM 480 502 950
TMhelix TMHMM 116 138 950
PS50850 Prosite Profiles IPR020846 Major facilitator superfamily domain 83 506 55
PTHR23502 HMMPANTHER 18 521 22
PTHR23502:SF36 HMMPANTHER 18 521 3
1.20.1250.20 Gene3D Major facilitator superfamily domain 59 262 72
1.20.1250.20 Gene3D Major facilitator superfamily domain 307 499 72
SSF103473 SuperFamily IPR020846 Major facilitator superfamily domain 66 500 76

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 111.19 Da
Charge 4.50
Codon Adaptation Index 0.49
Isoelectric point 7.47
Molecular weight 58.48 kDa
Number of residues 526
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phaseS36
present during mitotic M phaseS34
present during mitotic M phaseS7
present during mitotic M phaseS33
present during mitotic M phaseS16
present during mitotic M phaseS12
S7, S12, S16, S44
level fluctuates during mitotic cell cycleS7
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S36 PMID:21712547
present during mitotic M phase experimental evidence S34 PMID:21712547
present during mitotic M phase experimental evidence S7 PMID:21712547
present during mitotic M phase experimental evidence S33 PMID:21712547
present during mitotic M phase experimental evidence S16 PMID:21712547
present during mitotic M phase experimental evidence S12 PMID:21712547
IDA S7 PMID:25720772
experimental evidence S16 PMID:24763107
IDA S16 PMID:25720772
experimental evidence S12 PMID:24763107
IDA S12 PMID:25720772
experimental evidence S44 PMID:24763107
level fluctuates during mitotic cell cycle experimental evidence S7 PMID:24763107
O-phospho-L-threonine 1085
present during mitotic M phaseT14
present during mitotic M phaseT31
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T14 PMID:21712547
present during mitotic M phase experimental evidence T31 PMID:21712547
ubiquitinylated lysineK282, K516 512
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence K516 PMID:26412298
mass spectrometry evidence K282 PMID:26412298
IDA PMID:26412298
O-phosphorylated residueS7,S10, S7,S12, S7,T14, S34,S44 2457
Annotation ExtensionEvidenceResidueReference
IDA S7,S12 PMID:25720772
IDA S34,S44 PMID:25720772
IDA S7,S10 PMID:25720772
IDA S7,T14 PMID:25720772
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelpresent during GO:0036225Western blot evidencePMID:15701794
absent during GO:0071301Western blot evidencePMID:15701794
transcriptiondecreased in presence of thiamine Western blot evidencePMID:8675019
RNA levelabsent during GO:0071301Northern assay evidencePMID:15701794
present in presence of thiazole Northern assay evidencePMID:8675019
present during GO:0036225Northern assay evidencePMID:15701794
decreased in presence of aminopyrimidine Northern assay evidencePMID:8675019
present in presence of amiloride Northern assay evidencePMID:8675019
decreased during GO:0071301Northern assay evidencePMID:8675019

Quantitative Gene Expression

View graphical display of gene expression data for bsu1 (SPAC17A2.01)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4760during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
6419during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5481during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5613during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8505.91during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
5840during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
23during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene name conflict19
S. cerevisiae PN uptake is mediated by the unrelated transporter Tpn1p1
Taxonomic Conservation
DescriptionQualifierReferenceCount
orthologs cannot be distinguished124
conserved in fungi4607
conserved in eukaryotes4517
Orthologs

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC17A2.01 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withthi9thiamine transmembrane transporter Thi9 Synthetic Growth DefectPMID:18201975
synthetic growth defect withnmt14-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase Nmt1 Synthetic Growth DefectPMID:8675019
External References
Database Identifier Description
NBRP SPAC17A2.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17A2.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17A2.01 BioGRID Interaction Datasets
Expression Viewer SPAC17A2.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17A2.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17A2.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17A2.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17A2.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17A2.01 Transcriptome Viewer (Bähler Lab)
GEO SPAC17A2.01 GEO profiles
PInt SPAC17A2.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC17A2.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC17A2.01 Fission yeast phenotypic data & analysis
Cyclebase SPAC17A2.01.1 Cell Cycle Data
SPD / RIKEN45/45C01Orfeome Localization Data
UniProtKB/SwissProtP33532Vitamin B6 transporter bsu1
ModBaseP33532Database of comparative protein structure models
STRINGP33532Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001713032972h- high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1 (bsu1), mRNA
RefSeq PeptideXP_001713084high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1
European Nucleotide ArchiveCAA78411.1ENA Protein Mapping
European Nucleotide ArchiveCAB11084.1ENA Protein Mapping
UniParcUPI0000126F1EUniProt Archive

Literature for bsu1

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016