bsu1 (SPAC17A2.01)

Gene Standard Namebsu1 Characterisation Statuspublished
Systematic IDSPAC17A2.01 Feature Typeprotein coding
Synonymscar1, sod1, SPAC1B1.05 Name Descriptionvitamin B Six Uptake,
Changed Amiloride Resistance
Producthigh-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1 Product Size526aa, 58.48 kDa
Genomic Location Chromosome I, 3549682-3552067 (2386nt); CDS:3550152-3551732 (1581nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031925pyridoxal transmembrane transporter activityIDAPMID:157017943
GO:0031927pyridoxamine transmembrane transporter activityIDAPMID:157017943
GO:0031928pyridoxine transmembrane transporter activityIDAPMID:157017943
GO:0015234thiamine transmembrane transporter activityIGIthi9PMID:182019753
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1903090pyridoxal transmembrane transportIDAPMID:157017943
GO:1903091pyridoxamine transmembrane transportIDAPMID:157017943
GO:1903092pyridoxine transmembrane transportIDAPMID:157017943
GO:0071934thiamine transmembrane transport3
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371018
GO:0005886plasma membraneIDAPMID:15701794244
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001164normal growth on glucose carbon sourceCell growth assaybsu1ΔNullPECO:0000102, PECO:0000126PMID:15701794165
FYPO:0001357normal vegetative cell population growthCell growth assaycar1-2PECO:0000108, PECO:0000126PMID:8675019618
Cell growth assaycar1-5PECO:0000108, PECO:0000126PMID:8675019
Cell growth assaybsu1ΔNullPECO:0000108, PECO:0000126PMID:8675019
Cell growth assaycar1-2PECO:0000107, PECO:0000126PMID:8675019
Cell growth assaycar1-5PECO:0000107, PECO:0000126PMID:8675019
Cell growth assaybsu1ΔNullPECO:0000107, PECO:0000126PMID:8675019
Cell growth assaycar1-2PECO:0000106, PECO:0000126PMID:8675019
Cell growth assaycar1-5PECO:0000106, PECO:0000126PMID:8675019
Cell growth assaybsu1ΔNullPECO:0000106, PECO:0000126PMID:8675019
FYPO:0000125resistance to amilorideCell growth assaycar1-2PMID:86750192
Cell growth assaycar1-5PMID:8675019
Cell growth assaybsu1ΔNullPECO:0000102, PECO:0000126PMID:15701794
Cell growth assaybsu1ΔNullPMID:8675019
Cell growth assaybsu1ΔNullPECO:0000126, PECO:0000103PMID:8246883
FYPO:0002060viable vegetative cell populationMicroscopybsu1ΔNullPMID:204732893751
Microscopybsu1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003366decreased pyridoxine importSubstance quantification evidencebsu1ΔNullPMID:157017941
FYPO:0002805normal sodium importSubstance quantification evidencebsu1ΔNullPMID:82468831
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopybsu1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
FYPO affected by mutation in snz1 pyridoxine biosynthesis protein PMID:15701794
FYPO affected by mutation in tnr3 thiamine diphosphokinase Tnr3/ Nudix hydrolase fusion protein PMID:8675019
Ensembl transcript structure with UTRs, exons and introns


Exon Start End
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07690 Pfam IPR011701 Major facilitator superfamily 87 463 50
TMhelix TMHMM 343 365 959
TMhelix TMHMM 308 330 959
TMhelix TMHMM 480 502 959
TMhelix TMHMM 116 138 959
TMhelix TMHMM 148 166 959
TMhelix TMHMM 239 261 959
TMhelix TMHMM 173 195 959
TMhelix TMHMM 385 407 959
TMhelix TMHMM 443 465 959
TMhelix TMHMM 210 232 959
TMhelix TMHMM 81 103 959
PS50850 Prosite Profiles IPR020846 Major facilitator superfamily domain 83 506 55
PTHR24004:SF18 HMMPANTHER 13 510 16
PTHR24004 HMMPANTHER 13 510 17
1.20.1250.20 Gene3D 59 262 72
1.20.1250.20 Gene3D 307 499 72
SSF103473 SuperFamily IPR016196 Major facilitator superfamily domain, general substrate transporter 66 500 76

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount

Protein Properties

Ave. residue weight 111.19 Da
Charge 4.50
Isoelectric point 7.47
Molecular weight 58.48 kDa
Number of residues 526

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
present during mitotic M phase
present during mitotic M phaseS36PMID:21712547
present during mitotic M phaseS33PMID:21712547
present during mitotic M phaseS7PMID:21712547
present during mitotic M phaseS12PMID:21712547
level fluctuates during mitotic cell cycleS7PMID:24763107
present during mitotic M phaseS16PMID:21712547
present during mitotic M phase
present during mitotic M phaseT14PMID:21712547
Gene Expression

Qualitative Gene Expression

protein levelabsent during GO:0071301Western blot evidencePMID:15701794
present during GO:0036225Western blot evidencePMID:15701794
transcriptiondecreased in presence of thiamine Western blot evidencePMID:8675019
RNA levelpresent in presence of thiazole Northern assay evidencePMID:8675019
absent during GO:0071301Northern assay evidencePMID:15701794
decreased during GO:0071301Northern assay evidencePMID:8675019
decreased in presence of aminopyrimidine Northern assay evidencePMID:8675019
present during GO:0036225Northern assay evidencePMID:15701794
present in presence of amiloride Northern assay evidencePMID:8675019

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4760during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
6419during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
5481during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
5613during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
8505.91during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
5840during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
23during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
1.8during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
gene name conflict19
S. cerevisiae PN uptake is mediated by the unrelated transporter Tpn1p1
Species Distribution
orthologs cannot be distinguished131
conserved in fungi4599
conserved in eukaryotes4514

Orthologs in Compara

Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
nmt14-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase Nmt1 Synthetic Growth DefectPMID:8675019
thi9thiamine transporter Thi9 Synthetic Growth DefectPMID:18201975
External References
Database Identifier Description
NBRP SPAC17A2.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17A2.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17A2.01 BioGRID Interaction Datasets
Expression Viewer SPAC17A2.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17A2.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17A2.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17A2.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17A2.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17A2.01 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC17A2.01 Cell Cycle Data
GEO SPAC17A2.01 GEO profiles
PInt SPAC17A2.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC17A2.01 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN45/45C01Orfeome Localization Data
UniProtKB/SwissProtP33532Vitamin B6 transporter bsu1
ModBaseP33532Database of comparative protein structure models
STRINGP33532Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001713032972h- high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1 (bsu1), mRNA
RefSeq PeptideXP_001713084high-affinity import carrier for pyridoxine, pyridoxal, and pyridoxamine Bsu1
European Nucleotide ArchiveCAA78411.1ENA Protein Mapping
European Nucleotide ArchiveCAB11084.1ENA Protein Mapping
UniParcUPI0000126F1EUniProt Archive

Literature for bsu1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014