rad25 (SPAC17A2.13c)

Gene Standard Namerad25 ChromosomeI
Systematic IDSPAC17A2.13c Gene Start3584899
Synonyms Gene End3583128
Product14-3-3 protein Rad25 Gene Length1772
Feature Typeprotein coding CDS Start3584779
Name Description CDS End3583250
Characterisation Statuspublished CDS Length1530

Ensembl Gene Location
  1. SPNCRNA.917
  2. SPAC17A2.11
  3. SPNCRNA.918
  4. rrp1
  5. rad25
  6. SPNCRNA.919
  7. SPNCRNA.920
  8. SPAC17A2.14
  9. SPNCRNA.600
  10. tco1
  11. SPAC17G6.03
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000059abnormal mitotic cell cycle progressionnot recorded (overexpression)unknownPMID:12237855
FYPO:0002060viable vegetative cell populationMicroscopyrad25delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0004860protein kinase inhibitor activityIGIcdc25PMID:9832516
GO:0005515protein bindingIPIplc1PMID:9613582
IPImei2PMID:11818066
IPIbyr2PMID:12242289
IPIcdc25PMID:9774107
IPIcid13PMID:12062100
GO:0046982protein heterodimerization activityIPIrad24PMID:12242289
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000077DNA damage checkpointIMPPMID:8036497
GO:0006281DNA repairIEAUniProtKB:KW-0234GO_REF:0000037
GO:0007095mitotic G2 DNA damage checkpointIMPPMID:8036497
GO:0034613cellular protein localization
has_input byr2IMPPMID:12242289
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0005634nucleusIDAPMID:16823372
GO:0005829cytosolIDAPMID:16823372
GO:0032153cell division siteIDAPMID:16823372
GO:0051286cell tipIDAPMID:16823372
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
135848993584713
235839953583128

References

Region Start End Reference
five_prime_UTR35848993584780PMID:21511999
five_prime_UTR35848993584780PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00244 IPR023410 Pfam 14-3-3 protein 5 238 2
SM00101 IPR023410 SMART 14-3-3 domain 5 246 2
PS00796 IPR023409 Prosite Patterns 14-3-3 protein 43 53 2
PS00797 IPR023409 Prosite Patterns 14-3-3 protein 215 234 2
G3DSA:1.20.190.20 IPR023410 Gene3D 2 233 2
SSF48445 IPR023410 SuperFamily 3 235 2
PIRSF000868 IPR000308 PIRSF 14-3-3 protein 3 245 2
PTHR18860 IPR000308 hmmpanther 14-3-3 protein 3 256 2
PTHR18860:SF0 hmmpanther 3 256 2
Coil ncoils Rabaptin coiled-coil domain 11 32 974
PR00305 IPR000308 PRINTS 14-3-3 protein 152 178 2
PR00305 IPR000308 PRINTS 14-3-3 protein 86 110 2
PR00305 IPR000308 PRINTS 14-3-3 protein 37 66 2
PR00305 IPR000308 PRINTS 14-3-3 protein 206 235 2
PR00305 IPR000308 PRINTS 14-3-3 protein 117 139 2
PR00305 IPR000308 PRINTS 14-3-3 protein 179 205 2

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residuePMID:19547744

Protein Properties

Ave. residue weight 112.48 Da
Charge -15.00
Isoelectric point 4.49
Molecular weight 30.37 kDa
Number of residues 270
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencePECO:0000014,
PECO:0000005		population_wide48394.43PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide60743.14PMID:23101633
RNA levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide6.3PMID:23101633
experimental evidencePECO:0000014,
PECO:0000005		population_wide19PMID:23101633
Pathway
DescriptionQualifierReference
negative regulator of the Ras1-Byr2 signalling pathway, acting downstream of Ras1 and upstream of Byr2 PMID:12242289
Species Distribution
DescriptionQualifierReference
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
shf1small histone ubiquitination factor Shf1 Positive GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Dosage RescuePMID:22496451
Negative GeneticPMID:22681890
nup60nucleoporin Nup60 Negative GeneticPMID:22681890
SPBC839.02arrestin Aly1 related, implicated in endocytosis Negative GeneticPMID:22681890
rok1ATP-dependent RNA helicase Rok1 (predicted) Negative GeneticPMID:18818364
fsv1SNARE Fsv1 Negative GeneticPMID:22681890
rst2transcription factor Rst2 Dosage RescuePMID:22496451
tma23ribosome biogenesis protein Tma23 (predicted) Negative GeneticPMID:18818364
apt1adenine phosphoribosyltransferase (APRT) (predicted) Negative GeneticPMID:22681890
git3G-protein coupled receptor Git3 Negative GeneticPMID:22681890
set1histone lysine methyltransferase Set1 Positive GeneticPMID:18818364
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:18818364
hip1hira protein, histone chaperone Hip1 Positive GeneticPMID:18818364
SPAC1093.03inositol polyphosphate phosphatase (predicted) Positive GeneticPMID:18818364
hsp9heat shock protein Hsp9 Dosage RescuePMID:22182414
apc10anaphase-promoting complex subunit Apc10 Dosage RescuePMID:15507118
cut14condensin complex subunit Cut14 Synthetic LethalityPMID:22645654
elp1elongator subunit Elp1 (predicted) Positive GeneticPMID:22681890
lsg1Lsk1 complex gamma subunit (predicted) Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Positive GeneticPMID:18818364
rad2414-3-3 protein Rad24 Dosage RescuePMID:22496451
spf1Set1C PHD Finger protein Spf1 Positive GeneticPMID:18818364
dad1DASH complex subunit Dad1 Synthetic Growth DefectPMID:18931302
ras1GTPase Ras1 Phenotypic SuppressionPMID:12242289
cdc25M phase inducer phosphatase Cdc25 Dosage RescuePMID:9832516
SPBC31F10.02acyl-CoA hydrolase (predicted) Negative GeneticPMID:22681890
SPCC550.03cSki complex RNA helicase Ski2 (predicted) Negative GeneticPMID:22681890
pst2Clr6 histone deacetylase complex subunit Pst2 Phenotypic EnhancementPMID:19547744
cut9anaphase-promoting complex subunit Cut9 Dosage RescuePMID:15507118
Dosage RescuePMID:12653962
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
eme1Holliday junction resolvase subunit Eme1 Negative GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
sat1Golgi membrane exchange factor subunit Sat1 (predicted) Negative GeneticPMID:22681890
ccr1NADPH-cytochrome p450 reductase Positive GeneticPMID:22681890
sds23PP2A-type phosphatase inhibitor Sds23/Moc1 Positive GeneticPMID:22681890
SPAC2F7.17mitochondrial peptide chain release factor (predicted) Positive GeneticPMID:22681890
gld1mitochondrial glycerol dehydrogenase Gld1 Positive GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
pds5cohesin-associated protein Pds5 Positive GeneticPMID:22681890
pka1cAMP-dependent protein kinase catalytic subunit Pka1 Dosage RescuePMID:19584544
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-MSPMID:23297348
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
plc1phosphoinositide phospholipase C Plc1 Two-hybridPMID:9613582
hsp9heat shock protein Hsp9 Affinity Capture-WesternPMID:22182414
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
ago1argonaute Affinity Capture-WesternPMID:17043360
cdc25M phase inducer phosphatase Cdc25 Affinity Capture-WesternPMID:10090724
Affinity Capture-WesternPMID:22182414
prz1calcineurin responsive transcription factor Prz1 Affinity Capture-WesternPMID:22496451
chk1Chk1 protein kinase Two-hybridPMID:10090724
Affinity Capture-Western
lrp1leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
byr2MAP kinase kinase kinase Byr2 Affinity Capture-WesternPMID:12242289
Reconstituted Complex
cyk3cytokinesis protein Cyk3 (predicted) Affinity Capture-MSPMID:20603077
rad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:20231270
Affinity Capture-WesternPMID:12242289
ssp1serine/threonine protein kinase Ssp1 Affinity Capture-MSPMID:19371376
cid13poly(A) polymerase Cid13 Affinity Capture-MSPMID:12062100
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External References
Database Identifier Description
NBRP SPAC17A2.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17A2.13c Retrieval of eukaryotic orthologs
BioGrid SPAC17A2.13c BioGRID Interaction Datasets
Bähler Lab SPAC17A2.13c Cell Cycle Expression Profile
Bähler Lab SPAC17A2.13c Meiosis/Sporulation Expression Profies
Bähler Lab SPAC17A2.13c Pheromone response/mating expression profiles
Bähler Lab SPAC17A2.13c Environmental stress expression profiles
Bähler Lab SPAC17A2.13c Bähler Lab Transcriptome Viewer
Cyclebase SPAC17A2.13c Cell Cycle Data
PInt SPAC17A2.13c Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC17A2.13c Entrez Gene
WikiGene254225214-3-3 protein Rad25
Uniprot_genenameRAD25
EntrezGene254225214-3-3 protein Rad25
UniProtKB/SwissProtP42657DNA damage checkpoint protein rad25
ModBaseP42657Database of comparative protein structure models
Pfam Protein DomainsP42657Pfam Domain Arrangement
RefSeq PeptideNP_59424714-3-3 protein Rad25
RefSeq mRNANM_001019670972h- 14-3-3 protein Rad25 (rad25), mRNA
European Nucleotide ArchiveCAA55796ENA Protein Mapping
European Nucleotide ArchiveCAB16570ENA Protein Mapping
SPD / RIKEN24/24G12Orfeome Localization Data
UniParcUPI000013301EUniProt Archive

Literature for rad25
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013