rec12 (SPAC17A5.11)


Gene Standard Namerec12 Characterisation Statuspublished
Systematic IDSPAC17A5.11 Feature Typeprotein coding
Synonymsspo11 Name Description
Productmeiotic recombination endonuclease Rec12 Product Size345aa, 39.40 kDa
Genomic Location Chromosome I, 1772925-1774203 (1279nt); CDS:1772925-1774203 (1279nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding557
Annotation ExtensionEvidenceWith/FromReference
DNA binding352
Annotation ExtensionEvidenceWith/FromReference
endodeoxyribonuclease activity, producing 3'-phosphomonoesters4
Annotation ExtensionEvidenceWith/FromReference
metal ion binding750
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
nuclear chromosome490
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased frequency of meiosisrec12Δ14
decreased spore germination frequencyrec12-Δ17169
rec12-117 (G202E)
rec12Δ
normal growth during cellular response to UVrec12Δ79
normal growth on methyl methanesulfonaterec12Δ58
normal sporulation frequencyrec12Δ14
sensitive to hydroxyurearec12Δ594
sensitive to methyl methanesulfonaterec12Δ331
viable vegetative cell populationrec12Δ3850

Cell Phenotype

Term NameGenotypesCount
abnormal chromosome segregation during meiosis I with split kinetochore and equational sister chromatid separationrec12-Y98F (Y98F)3
rec12Δ
abnormal chromosome segregation during meiosis I with split kinetochore and normal chromosome separationrec12Δ5
rec12-Y98F (Y98F)
abnormal linear element morphologyrec12Δ6
abnormal meiotic DNA double-strand break formationrec12Δ11
abnormal meiotic homologous chromosome biorientationrec12Δ3
abnormal meiotic homologous chromosome biorientation with abnormal kinetochore orientation1
affecting ark1rec12Δ
abnormal mitotic spindle elongationrec12Δ25
abnormal sporulation resulting in formation of ascus containing non-uniform sporesrec12Δ11
abnormal sporulation resulting in formation of ascus with fewer than four sporesrec12Δ78
abolished meiotic DNA double-strand break formationrec12Δ9
decreased homologous chromosome pairingrec12Δ4
decreased intergenic meiotic recombinationrec12Δ30
decreased intragenic meiotic recombination37
affecting ade6rec12Δ
decreased meiotic recombinationrec12Δ56
decreased protein phosphorylation during meiotic cell cycle4
affecting mek1rec12Δ
decreased rate of premeiotic DNA replicationrec12Δ2
delayed onset of protein localization from kinetochore to spindle during meiosis I1
affects localization of ark1rec12Δ
globally decreased meiotic recombinationrec12-1521
rec12Δ
increased activation of meiosis I spindle assembly checkpointrec12Δ2
meiosis I metaphase/anaphase transition delayrec12Δ5
normal chromosome disjunction at meiosis Irec12-Y98F (Y98F)5
rec12Δ
normal horsetail movementrec12Δ5
normal linear element morphologyrec12-1526
normal meiotic sister chromatid cohesionrec12Δ7
normal meiotic sister chromatid cohesion at centromererec12Δ3
normal meiotic telomere clusteringrec12Δ9
normal premeiotic DNA replicationrec12-117 (G202E)25
normal protein localization to linear element4
affecting hop1rec12-152
normal rate of homologous chromosome pairing at cis-acting homologous chromosome pairing regionrec12Δ2
premature meiosisrec12Δ8
unequal homologous chromosome segregationrec12Δ14
viable vegetative cell with normal cell morphologyrec12Δ3102

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased spore germination frequencymre11Δ, rec12Δ
rec12-117 (G202E), mre11-D65N (D65N)
ctp1Δ, rec12Δ
rad50Δ, rec12Δ
rec12-117 (G202E), rad50S (K81I)

Cell Phenotype

Term NameGenotypes
abnormal chromosome segregation during meiosis I with split kinetochore and equational sister chromatid separationmad2Δ, rec12Δ
abnormal chromosome segregation during meiosis I with split kinetochore and normal chromosome separationsgo1Δ, rec12Δ
ark1-as (analog-sensitive), rec12Δ
mad2Δ, rec12Δ
abnormal chromosome segregation during meiosis I with split kinetochore, lagging chromosomes, and normal chromosome separationsgo1Δ, rec12Δ
abolished meiotic DNA double-strand break formationctp1Δ, rec12Δ
decreased intragenic meiotic recombinationrec12-152, mus81Δ
decreased meiotic recombination at hotspotrad16Δ, rec12-152
rec12-152, rad50S (K81I)
mre11-D65N (D65N), rec12Δ
globally decreased meiotic recombinationmre11-D65N (D65N), rec12Δ
rec12-152, rad50S (K81I)
rad16Δ, rec12-152
normal duration of meiosis Imad2Δ, rec12Δ
normal meiotic sister chromatid cohesionRDRD (E369R, E370R, R373E, E379R, E381R, R384E), rec12Δ
normal onset of meiosis Imcp7Δ, rec12Δ
sister chromatid separation during meiosis Irec12Δ, moa1-101A (102-172)
sgo1Δ, rec12Δ
moa1Δ, rec12Δ
plo-tev(tev-site@AA341) (protease cleavable), rec12Δ
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1772925..1773205, 1773264..1773372, 1773423..1773500, 1773565..1773693, 1773763..1774203
Intron1773206..1773263, 1773373..1773422, 1773501..1773564, 1773694..1773762
mRNA1772925..1774203
CDS1772925..1773205, 1773264..1773372, 1773423..1773500, 1773565..1773693, 1773763..1774203
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF04406 Pfam IPR013049 Spo11/DNA topoisomerase VI, subunit A, N-terminal 70 130 1
PTHR10848 HMMPANTHER IPR002815 Spo11/DNA topoisomerase VI subunit A 10 325 1
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 4 132 46
3.40.1360.10 Gene3D Spo11/DNA topoisomerase VI subunit A 134 336 1
0036260 SuperFamily IPR002815 Spo11/DNA topoisomerase VI subunit A 70 337 1
PR01550 PRINTS IPR002815 Spo11/DNA topoisomerase VI subunit A 196 217 1
PR01550 PRINTS IPR002815 Spo11/DNA topoisomerase VI subunit A 93 104 1
PR01550 PRINTS IPR002815 Spo11/DNA topoisomerase VI subunit A 125 140 1
PR01550 PRINTS IPR002815 Spo11/DNA topoisomerase VI subunit A 229 245 1
PR01550 PRINTS IPR002815 Spo11/DNA topoisomerase VI subunit A 175 192 1
PR01550 PRINTS IPR002815 Spo11/DNA topoisomerase VI subunit A 107 124 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.20 Da
Charge 14.00
Codon Adaptation Index 0.38
Isoelectric point 9.72
Molecular weight 39.40 kDa
Number of residues 345
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0051321Northern assay evidencePMID:8005432
increased during GO:0051321experimental evidencePMID:8005432

Quantitative Gene Expression

View graphical display of gene expression data for rec12 (SPAC17A5.11)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.11during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.082during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in archaea242
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC17A5.11 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withrec6meiotic recombination protein Rec6 Two-hybridPMID:22841486
affinity capturesrec14Ski complex subunit Rec14 Affinity Capture-WesternPMID:22841486
binds activation domain construct withrec14Ski complex subunit Rec14 Two-hybridPMID:22841486
affinity captured byrec14Ski complex subunit Rec14 Affinity Capture-WesternPMID:22841486
affinity captured byrec6meiotic recombination protein Rec6 Affinity Capture-WesternPMID:22841486
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC17A5.11 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetically rescueseme1Holliday junction resolvase subunit Eme1 Synthetic RescuePMID:11719193
synthetically rescuesmcp7meiosis specific coiled-coil protein Mcp7 Synthetic RescuePMID:15210864
synthetically rescuesmus81Holliday junction resolvase subunit Mus81 Synthetic RescuePMID:11719193
synthetically rescueskms1meiotic spindle pole body protein Kms1 Synthetic RescuePMID:24312672
synthetically rescuesnse6Smc5-6 complex non-SMC subunit Nse6 Synthetic RescuePMID:22855558
synthetically rescuesfbh1DNA helicase I, ubiquitin ligase F-box adaptor Fbh1 Synthetic RescuePMID:21149262
overexpression rescuesmre11Mre11 nuclease Dosage RescuePMID:17936710
overexpression rescuesctp1CtIP-related endonuclease Dosage RescuePMID:17936710
phenotype enhanced bymeu13Tat binding protein 1(TBP-1)-interacting protein (TBPIP) homolog (predicted) Phenotypic EnhancementPMID:11447128
phenotype enhanced bydcc1Ctf18 RFC-like complex subunit Dcc1 Phenotypic EnhancementPMID:19370027
phenotype enhanced bypds5mitotic and meiotic cohesin complex subunit Pds5 Phenotypic EnhancementPMID:19370027
phenotype enhanced bydlc1dynein light chain Dlc1 Phenotypic EnhancementPMID:15802518
phenotype enhanced byctf18RFC-like complex subunit Ctf18 Phenotypic EnhancementPMID:19370027
phenotype enhanced bysgo1meiotic inner centromere protein, shugoshin, Sgo1 Phenotypic EnhancementPMID:21423721
phenotype enhanced bydhc1minus-end directed microtubule motor, dynein heavy chain Dhc1 Phenotypic EnhancementPMID:15802518
phenotype enhanced bymoa1meiotic cohesin complex associated protein (Meikin) Moa1 Phenotypic EnhancementPMID:19370027
rescuesrec8meiotic cohesin complex subunit Rec8 Phenotypic SuppressionPMID:14532136
rescuesdcr1dicer Phenotypic SuppressionPMID:20421495
rescuesrad50DNA repair protein Rad50 Phenotypic SuppressionPMID:15238514
rescuessfr1Swi five-dependent recombination mediator Sfr1 Phenotypic SuppressionPMID:22723423
rescuesmre11Mre11 nuclease Phenotypic SuppressionPMID:15238514
rescuesfml1ATP-dependent 3' to 5' DNA helicase, FANCM ortholog Fml1 Phenotypic SuppressionPMID:22723423
rescuesrad16DNA repair endonuclease XPF Phenotypic SuppressionPMID:25293972
rescuesmus81Holliday junction resolvase subunit Mus81 Phenotypic SuppressionPMID:22723423
rescuesmeu13Tat binding protein 1(TBP-1)-interacting protein (TBPIP) homolog (predicted) Phenotypic SuppressionPMID:12482912
negative genetic interaction withSPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:18818364
negative genetic interaction withmca1transcription factor, zf-fungal binuclear cluster type Mca1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgit3G-protein coupled receptor Git3 Negative GeneticPMID:22681890
negative genetic interaction withsat1Golgi membrane exchange factor subunit Sat1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBPB10D8.05ctransmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpub1HECT-type ubiquitin-protein ligase E3 Pub1 Negative GeneticPMID:18818364
negative genetic interaction withser2phosphoserine phosphatase Ser2 (predicted) Negative GeneticPMID:18818364
negative genetic interaction withmed20mediator complex subunit Med20 Negative GeneticPMID:18818364
negative genetic interaction witheme1Holliday junction resolvase subunit Eme1 Negative GeneticPMID:18818364
negative genetic interaction withalb1pre-60S shuttling factor Alb1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcrn1actin binding protein, coronin Crn1 Negative GeneticPMID:22681890
negative genetic interaction withent3ENTH/VHS domain protein Ent3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrad51RecA family recombinase Rad51/Rhp51 Negative GeneticPMID:18818364
negative genetic interaction withmre11Mre11 nuclease Negative GeneticPMID:18818364
negative genetic interaction withgpd1glycerol-3-phosphate dehydrogenase Gpd1 Negative GeneticPMID:22681890
negative genetic interaction withdep1Sds3-like family protein Dep1 Negative GeneticPMID:22681890
positive genetic interaction withSPAC3H5.08cWD repeat protein, human WDR44 family Positive GeneticPMID:22681890
positive genetic interaction withvps3CORVET complex subunit, GTPase regulator Vps3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPCC4B3.13MatE family transmembrane transporter (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPCC306.11Schizosaccharomyces specific protein Positive GeneticPMID:22681890
positive genetic interaction withhmt2sulfide-quinone oxidoreductase Positive GeneticPMID:22681890
positive genetic interaction withfkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Positive GeneticPMID:22681890
rescued bymad2mitotic spindle checkpoint protein Mad2 Phenotypic SuppressionPMID:21423721
rescued byrad50DNA repair protein Rad50 Phenotypic SuppressionPMID:15654094
rescued byrad2FEN-1 endonuclease Rad2 Phenotypic SuppressionPMID:16118186
rescued bymre11Mre11 nuclease Phenotypic SuppressionPMID:15654094
synthetically rescued bychk1Chk1 protein kinase Synthetic RescuePMID:15689488
synthetically rescued bynse1Smc5-6 complex ubiquitin-protein ligase E3 subunit Nse1 Synthetic RescuePMID:15331764
synthetically rescued bycds1replication checkpoint kinase Cds1 Synthetic RescuePMID:15689488
synthetically rescued byrad3ATR checkpoint kinase Rad3 Synthetic RescuePMID:15689488
enhances phenotype ofsgo1meiotic inner centromere protein, shugoshin, Sgo1 Phenotypic EnhancementPMID:21920317
enhances phenotype ofpat1serine/threonine protein kinase Ran1/Pat1 Phenotypic EnhancementPMID:12727894
enhances phenotype ofmad2mitotic spindle checkpoint protein Mad2 Phenotypic EnhancementPMID:21423721
synthetic growth defect withrec10meiotic recombination protein Rec10 Synthetic Growth DefectPMID:21387406
External References
Database Identifier Description
NBRP SPAC17A5.11 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17A5.11 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17A5.11 BioGRID Interaction Datasets
Expression Viewer SPAC17A5.11 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17A5.11 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17A5.11 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17A5.11 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17A5.11 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17A5.11 Transcriptome Viewer (Bähler Lab)
GEO SPAC17A5.11 GEO profiles
PInt SPAC17A5.11 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC17A5.11 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC17A5.11 Fission yeast phenotypic data & analysis
Cyclebase SPAC17A5.11.1 Cell Cycle Data
SPD / RIKEN20/20E02Orfeome Localization Data
UniProtKB/SwissProtP40384Meiotic recombination protein rec12
ModBaseP40384Database of comparative protein structure models
STRINGP40384Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593479endonuclease Rec12
RefSeq mRNANM_001018912972h- endonuclease Rec12 (rec12), mRNA
European Nucleotide ArchiveAF195027ENA EMBL mapping
European Nucleotide ArchiveAAA35332.1ENA Protein Mapping
European Nucleotide ArchiveCAB11511.1ENA Protein Mapping
UniParcUPI00001334ACUniProt Archive

Literature for rec12

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016