gus1 (SPAC17A5.15c)

Gene Standard Namegus1 Characterisation Statuspublished
Systematic IDSPAC17A5.15c Feature Typeprotein coding
Synonymsers1 Name Description
Productcytoplasmic glutamate-tRNA ligase gus1 (predicted) Product Size716aa, 80.75 kDa
Genomic Location Chromosome I, 1785113-1782880 (2234nt); CDS:1785085-1782935 (2151nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
glutamate-tRNA ligase activity2
Annotation ExtensionEvidenceWith/FromReference
protein binding831
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translation473
Annotation ExtensionEvidenceWith/FromReference
glutamyl-tRNA aminoacylation2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaySPAC17A5.15c+ (wild type)OverexpressionPECO:0000103PMID:19502729147
FYPO:0002061inviable vegetative cell populationMicroscopygus1ΔNullPMID:204732891338
Microscopygus1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001043increased mating efficiencyCell growth assaySPAC17A5.15c+ (wild type)OverexpressionPMID:1950272926
FYPO:0002151inviable spore465
penetrance FYPO_EXT:0000001Microscopygus1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Cell growth assaygus1ΔNullPECO:0000005, PECO:0000137PMID:19502729
Ensembl transcript structure with UTRs, exons and introns


Exon Start End
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00749 Pfam IPR020058 Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain 206 510 3
PF03950 Pfam IPR020059 Glutamyl/glutaminyl-tRNA synthetase, class Ib, anti-codon binding domain 513 690 2
PF14497 Pfam Glutathione S-transferase, C-terminal 90 165 1
PS00178 Prosite Patterns IPR001412 Aminoacyl-tRNA synthetase, class I, conserved site 213 224 11
PTHR10119 HMMPANTHER IPR000924 Glutamyl/glutaminyl-tRNA synthetase 30 708 3
PTHR10119:SF16 HMMPANTHER 30 708 1
1.10.1160.10 Gene3D IPR020061 Glutamyl/glutaminyl-tRNA synthetase, class Ib, alpha-bundle domain 436 511 3
3.90.800.10 Gene3D 279 383 3 Gene3D IPR020056 Ribosomal protein L25/Gln-tRNA synthetase, beta-barrel domain 525 621 2
1.20.1050.10 Gene3D IPR010987 Glutathione S-transferase, C-terminal-like 104 183 11 Gene3D IPR020056 Ribosomal protein L25/Gln-tRNA synthetase, beta-barrel domain 622 707 2 Gene3D IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 386 435 39 Gene3D IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 206 275 39
SSF50715 SuperFamily IPR011035 Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain 513 707 2
SSF47616 SuperFamily IPR010987 Glutathione S-transferase, C-terminal-like 80 172 12
SSF52374 SuperFamily 206 519 24
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 210 222 3
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 239 252 3
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 384 394 3
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 400 408 3
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 224 235 3
TIGR00463 tigrfam IPR004526 Glutamyl-tRNA synthetase, archaeal/eukaryotic cytosolic 174 696 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.78 Da
Charge 2.50
Isoelectric point 6.77
Molecular weight 80.75 kDa
Number of residues 716

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00047O-phospho-L-threonineexperimental evidenceT655PMID:24763107692
level fluctuates during mitotic cell cycle
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
125199during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
122722during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
126567during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
126749during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
126025during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
23060.74during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
11052.77during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
17during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
2.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
slm9hira protein Slm9 Affinity Capture-MSPMID:20976105
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
moc3transcription factor Moc3 Two-hybridPMID:19502729
External References
Database Identifier Description
NBRP SPAC17A5.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17A5.15c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17A5.15c BioGRID Interaction Datasets
Expression Viewer SPAC17A5.15c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17A5.15c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17A5.15c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17A5.15c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17A5.15c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17A5.15c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC17A5.15c Cell Cycle Data
GEO SPAC17A5.15c GEO profiles
PInt SPAC17A5.15c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC17A5.15c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC17A5.15c Fission yeast phenotypic data & analysis
KEGGMAP00251KEGG Pathway Database
KEGGMAP00970KEGG Pathway Database
KEGGMAP00860KEGG Pathway Database
IntEnz6.1.1.17Integrated relational Enzyme database
Rhea6.1.1.17Annotated reactions database
SPD / RIKEN37/37E12Orfeome Localization Data
UniProtKB/SwissProtO13775Probable glutamate--tRNA ligase, cytoplasmic
ModBaseO13775Database of comparative protein structure models
STRINGO13775Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593483glutamate-tRNA ligase (predicted)
RefSeq mRNANM_001018916972h- glutamate-tRNA ligase (predicted) (SPAC17A5.15c), mRNA
European Nucleotide ArchiveCAB11515.1ENA Protein Mapping
UniParcUPI00001363CFUniProt Archive

Literature for gus1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014