gus1 (SPAC17A5.15c)


Gene Standard Namegus1 Characterisation Statuspublished
Systematic IDSPAC17A5.15c Feature Typeprotein coding
Synonymsers1 Name Description
Productcytoplasmic glutamate-tRNA ligase gus1 (predicted) Product Size716aa, 80.75 kDa
Genomic Location Chromosome I, 1785113-1782880 (2234nt); CDS:1785085-1782935 (2151nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0003723RNA bindingIEAUniProtKB-KW:KW-0694GO_REF:0000037627
GO:0004818glutamate-tRNA ligase activityISSUniProtKB:P46655GO_REF:00000012
GO:0005515protein bindingIPImoc3PMID:19502729887
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0002181cytoplasmic translationNASGO_REF:0000051445
GO:0006424glutamyl-tRNA aminoacylationISSUniProtKB:P46655GO_REF:00000012
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722317
GO:0005634nucleusIDAPMID:168233722730
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaySPAC17A5.15c+ (wild type)OverexpressionPECO:0000103PMID:1950272985
FYPO:0002061inviable vegetative cell populationMicroscopygus1ΔNullPECO:0000005, PECO:0000137PMID:236978061309
Microscopygus1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001043increased mating efficiencyCell growth assaySPAC17A5.15c+ (wild type)OverexpressionPMID:1950272911
FYPO:0002151inviable sporeMicroscopygus1ΔNullPECO:0000005, PECO:0000137PMID:23697806447
penetrance FYPO_EXT:0000001
Cell growth assaygus1ΔNullPECO:0000005, PECO:0000137PMID:19502729
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
117851131782880
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03950 Pfam IPR020059 Glutamyl/glutaminyl-tRNA synthetase 513 690 2
PF14497 Pfam 90 165 1
PF00749 Pfam IPR020058 Glutamyl/glutaminyl-tRNA synthetase 206 510 3
PS00178 Prosite Patterns IPR001412 Aminoacyl-tRNA synthetase, class I, conserved site 213 224 11
PTHR10119 HMMPANTHER IPR000924 Glutamyl/glutaminyl-tRNA synthetase 30 708 3
PTHR10119:SF16 HMMPANTHER 30 708 1
G3DSA:1.10.1160.10 Gene3D IPR020061 436 511 3
G3DSA:3.40.50.620 Gene3D IPR014729 206 275 39
G3DSA:3.40.50.620 Gene3D IPR014729 386 435 39
G3DSA:1.20.1050.10 Gene3D IPR010987 104 183 10
G3DSA:2.40.240.10 Gene3D IPR020056 622 707 2
G3DSA:2.40.240.10 Gene3D IPR020056 525 621 2
G3DSA:3.90.800.10 Gene3D 279 383 3
SSF50715 SuperFamily IPR011035 513 707 2
SSF47616 SuperFamily IPR010987 80 172 12
SSF52374 SuperFamily 206 519 24
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 400 408 3
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 239 252 3
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 384 394 3
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 224 235 3
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 210 222 3
TIGR00463 tigrfam IPR004526 Glutamyl-tRNA synthetase, archaeal/eukaryotic cytosolic 174 696 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.78 Da
Charge 2.50
Isoelectric point 6.77
Molecular weight 80.75 kDa
Number of residues 716
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
11052.77during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
23060.74during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
17during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3394
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
slm9hira protein Slm9 Affinity Capture-MSPMID:20976105
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
moc3transcription factor Moc3 Two-hybridPMID:19502729
External References
Database Identifier Description
NBRP SPAC17A5.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17A5.15c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17A5.15c BioGRID Interaction Datasets
Expression Viewer SPAC17A5.15c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17A5.15c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17A5.15c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17A5.15c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17A5.15c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17A5.15c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC17A5.15c Cell Cycle Data
GEO SPAC17A5.15c GEO profiles
PInt SPAC17A5.15c Protein-Protein Interaction Predictor (Bähler Lab)
SPD / RIKEN37/37E12Orfeome Localization Data
WikiGene2542408glutamate-tRNA ligase (predicted)
EntrezGene2542408glutamate-tRNA ligase (predicted)
IntEnz6.1.1.17Integrated relational Enzyme database
Rhea6.1.1.17Annotated reactions database
KEGGMAP00860
KEGGMAP00251
KEGGMAP00970
UniProtKB/SwissProtO13775Probable glutamate--tRNA ligase, cytoplasmic
ModBaseO13775Database of comparative protein structure models
StringO13775Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593483glutamate-tRNA ligase (predicted)
RefSeq mRNANM_001018916972h- glutamate-tRNA ligase (predicted) (SPAC17A5.15c), mRNA
European Nucleotide ArchiveCAB11515ENA Protein Mapping
UniParcUPI00001363CFUniProt Archive

Literature for gus1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014