gus1 (SPAC17A5.15c)


Gene Standard Namegus1 Characterisation Statuspublished
Systematic IDSPAC17A5.15c Feature Typeprotein coding
Synonymsers1 Name Description
Productcytoplasmic glutamate-tRNA ligase gus1 (predicted) Product Size716aa, 80.75 kDa
Genomic Location Chromosome I, 1785113-1782880 (2234nt); CDS:1785085-1782935 (2151nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
glutamate-tRNA ligase activity2
Annotation ExtensionEvidenceWith/FromReference
protein binding854
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translation462
Annotation ExtensionEvidenceWith/FromReference
glutamyl-tRNA aminoacylation2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
nucleus2695
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth on glucose carbon sourceSPAC17A5.15c+ (wild type)Overexpression256
increased mating efficiencySPAC17A5.15c+ (wild type)Overexpression28
inviable vegetative cell populationgus1ΔNull1437

Cell Phenotype

Term NameAlleleExpressionCount
inviable sporegus1ΔNull472
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons1785113..1782880
mRNA1785113..1782880
5' UTR1785113..1785086AU007805
CDS1785085..1782935
3' UTR1782934..1782880AU013934
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03950 Pfam IPR020059 Glutamyl/glutaminyl-tRNA synthetase, class Ib, anti-codon binding domain 513 690 2
PF14497 Pfam IPR004046 Glutathione S-transferase, C-terminal 90 165 1
PF00749 Pfam IPR020058 Glutamyl/glutaminyl-tRNA synthetase, class Ib, catalytic domain 206 510 3
PS00178 Prosite Patterns IPR001412 Aminoacyl-tRNA synthetase, class I, conserved site 213 224 11
PTHR10119:SF15 HMMPANTHER 83 715 1
PTHR10119 HMMPANTHER IPR000924 Glutamyl/glutaminyl-tRNA synthetase 83 715 3
2.40.240.10 Gene3D IPR020056 Ribosomal protein L25/Gln-tRNA synthetase, beta-barrel domain 525 621 2
3.90.800.10 Gene3D 279 383 3
3.40.50.620 Gene3D IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 386 435 39
3.40.50.620 Gene3D IPR014729 Rossmann-like alpha/beta/alpha sandwich fold 206 275 39
1.10.1160.10 Gene3D IPR020061 Glutamyl/glutaminyl-tRNA synthetase, class Ib, alpha-bundle domain 436 511 3
1.20.1050.10 Gene3D IPR010987 Glutathione S-transferase, C-terminal-like 104 183 11
2.40.240.10 Gene3D IPR020056 Ribosomal protein L25/Gln-tRNA synthetase, beta-barrel domain 622 707 2
SSF52374 SuperFamily 206 519 24
SSF47616 SuperFamily IPR010987 Glutathione S-transferase, C-terminal-like 80 172 12
SSF50715 SuperFamily IPR011035 Ribosomal protein L25/Gln-tRNA synthetase, anti-codon-binding domain 513 707 2
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 384 394 3
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 224 235 3
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 400 408 3
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 239 252 3
PR00987 PRINTS IPR000924 Glutamyl/glutaminyl-tRNA synthetase 210 222 3
TIGR00463 tigrfam IPR004526 Glutamyl-tRNA synthetase, archaeal/eukaryotic cytosolic 174 696 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.78 Da
Charge 2.50
Isoelectric point 6.77
Molecular weight 80.75 kDa
Number of residues 716
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-threonine 698
level fluctuates during mitotic cell cycleT655
Annotation ExtensionEvidenceResidueReference
level fluctuates during mitotic cell cycle experimental evidence T655 PMID:24763107
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
125199during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
122722during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
126567during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
126749during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
126025during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
23060.74during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
11052.77during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
17during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in bacteria1001
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withmoc3transcription factor Moc3 Two-hybridPMID:19502729
affinity captured byslm9hira protein Slm9 Affinity Capture-MSPMID:20976105
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
External References
Database Identifier Description
NBRP SPAC17A5.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17A5.15c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17A5.15c BioGRID Interaction Datasets
Expression Viewer SPAC17A5.15c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17A5.15c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17A5.15c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17A5.15c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17A5.15c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17A5.15c Transcriptome Viewer (Bähler Lab)
GEO SPAC17A5.15c GEO profiles
PInt SPAC17A5.15c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC17A5.15c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC17A5.15c Fission yeast phenotypic data & analysis
Cyclebase SPAC17A5.15c.1 Cell Cycle Data
IntEnz6.1.1.17Integrated relational Enzyme database
Rhea6.1.1.17Annotated reactions database
SPD / RIKEN37/37E12Orfeome Localization Data
KEGGMAP00251KEGG Pathway Database
KEGGMAP00970KEGG Pathway Database
KEGGMAP00860KEGG Pathway Database
UniProtKB/SwissProtO13775Probable glutamate--tRNA ligase, cytoplasmic
ModBaseO13775Database of comparative protein structure models
STRINGO13775Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593483glutamate-tRNA ligase (predicted)
RefSeq mRNANM_001018916972h- glutamate-tRNA ligase (predicted) (SPAC17A5.15c), mRNA
European Nucleotide ArchiveCAB11515.1ENA Protein Mapping
MetaCycPWY-5188Tetrapyrrole biosynthesis I (from glutamate)
KEGG_Enzyme00860+6.1.1.17Porphyrin and chlorophyll metabolism
KEGG_Enzyme00970+6.1.1.17Aminoacyl-tRNA biosynthesis
UniParcUPI00001363CFUniProt Archive

Literature for gus1

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015