pnu1 (SPAC17C9.08)

Gene Standard Namepnu1 ChromosomeI
Systematic IDSPAC17C9.08 Gene Start4486171
Synonymsend1, nuc1 Gene End4484695
Productmitochondrial endodeoxyribonuclease Pnu1 Gene Length1477
Feature Typeprotein coding CDS Start4485994
Name Description CDS End4484835
Characterisation Statuspublished CDS Length1160

Ensembl Gene Location
  1. cut8
  2. SPAC17C9.12
  3. SPAC17C9.11c
  4. stm1
  5. tim13
  6. pnu1
  7. alg8
  8. sam50
  9. pmc3
  10. tif471
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000160DNA fragmentationOthernot recorded (wild type)wild_typeOverexpressionPMID:18047809
FYPO:0000590normal sporulationCell growth assayend1-379 (unknown)unknownNot specifiedPMID:6090122
Cell growth assayend1-458 (unknown)unknownNot specifiedPMID:6090122
FYPO:0002037decreased endodeoxyribonuclease activityEnzyme assay dataend1-458 (unknown)unknownNot specifiedPECO:0000214PMID:6090122
Enzyme assay dataend1-379 (unknown)unknownNot specifiedPECO:0000214PMID:6090122
FYPO:0002060viable vegetative cell populationMicroscopypnu1delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0004518nuclease activityIMPPMID:17192844
GO:0004520endodeoxyribonuclease activityIMPPMID:6090122
ISSUniProtKB:P08466GO_REF:0000001
GO:0004529exodeoxyribonuclease activityISSUniProtKB:P08466GO_REF:0000001
GO:0046872metal ion bindingIEAUniProtKB:KW-0479GO_REF:0000037
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0000737DNA catabolic process, endonucleolyticIMPPMID:18047809
GO:0006308DNA catabolic processISSUniProtKB:P08466GO_REF:0000001
GO:0006310DNA recombinationISSUniProtKB:P08466GO_REF:0000001
GO:0006401RNA catabolic processIMPPMID:11872168
GO:0006995cellular response to nitrogen starvationIMPPMID:11872168
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0005739mitochondrionIDAPMID:11872168
GO:0005743mitochondrial inner membraneISSUniProtKB:P08466GO_REF:0000001
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
144861714485713
244855964485350
344852744484695

References

Region Start End Reference
three_prime_UTR44848344484695PMID:21511999
three_prime_UTR44848344484695PMID:21511999
five_prime_UTR44861714485995PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF01223 IPR001604 Pfam DNA/RNA non-specific endonuclease 79 290 1
SM00892 IPR001604 SMART DNA/RNA non-specific endonuclease 77 291 1
SM00477 IPR020821 SMART Extracellular Endonuclease, subunit A 78 291 1
TMhelix TMHMM 5 27 955
PS01070 IPR018524 Prosite Patterns DNA/RNA non-specific endonuclease 139 147 1
G3DSA:3.40.570.10 IPR020821 Gene3D 60 304 1
SSF54060 SuperFamily 62 310 1
PTHR13966 hmmpanther 63 321 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.13 Da
Charge 9.00
Isoelectric point 9.14
Molecular weight 36.43 kDa
Number of residues 322
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencePECO:0000014,
PECO:0000005		population_wide4918.24PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide10393.77PMID:23101633
RNA levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.79PMID:23101633
experimental evidencePECO:0000014,
PECO:0000005		population_wide3PMID:23101633
Warning
DescriptionQualifierReference
gene structure updated
Species Distribution
DescriptionQualifierReference
predominantly single copy (one to one)
conserved in fungi
conserved in eukaryotes
conserved in bacteria
conserved in metazoa
conserved in vertebrates
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:22681890
Negative GeneticPMID:21504829
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
elp3elongator complex, histone acetyltransferase subunit Elp3 (predicted) Negative GeneticPMID:22681890
ppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
atp11F1-ATPase chaperone Atp11 (predicted) Positive GeneticPMID:22681890
SPBC29A3.09cAAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
res2MBF transcription factor complex subunit Res2 Negative GeneticPMID:22681890
ask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
atg6beclin family protein, involved in autophagy Positive GeneticPMID:22681890
dsc5UBX domain containing protein required for Sre1 cleavage Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Positive GeneticPMID:22681890
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External References
Database Identifier Description
NBRP SPAC17C9.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17C9.08 Retrieval of eukaryotic orthologs
BioGrid SPAC17C9.08 BioGRID Interaction Datasets
Bähler Lab SPAC17C9.08 Cell Cycle Expression Profile
Bähler Lab SPAC17C9.08 Meiosis/Sporulation Expression Profies
Bähler Lab SPAC17C9.08 Pheromone response/mating expression profiles
Bähler Lab SPAC17C9.08 Environmental stress expression profiles
Bähler Lab SPAC17C9.08 Bähler Lab Transcriptome Viewer
Cyclebase SPAC17C9.08 Cell Cycle Data
PInt SPAC17C9.08 Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC17C9.08 Entrez Gene
WikiGene2542291mitochondrial endodeoxyribonuclease Pnu1
Uniprot_genenamePNU1
EntrezGene2542291mitochondrial endodeoxyribonuclease Pnu1
IntEnz3.1.30.-
UniProtKB/SwissProtQ10480Nuclease 1, mitochondrial
ModBaseQ10480Database of comparative protein structure models
Pfam Protein DomainsQ10480Pfam Domain Arrangement
RefSeq PeptideNP_594598mitochondrial endodeoxyribonuclease Pnu1
RefSeq mRNANM_001020026972h- mitochondrial endodeoxyribonuclease Pnu1 (pnu1), mRNA
European Nucleotide ArchiveBAB20882ENA Protein Mapping
European Nucleotide ArchiveCAA97354ENA Protein Mapping
SPD / RIKEN44/44A10Orfeome Localization Data
UniParcUPI00001687D6UniProt Archive

Literature for pnu1
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013