pnu1 (SPAC17C9.08)


Gene Standard Namepnu1 Characterisation Statuspublished
Systematic IDSPAC17C9.08 Feature Typeprotein coding
Synonymsend1, nuc1 Name Description
Productmitochondrial endodeoxyribonuclease Pnu1 Product Size322aa, 36.43 kDa
Genomic Location Chromosome I, 4486171-4484695 (1477nt); CDS:4485994-4484835 (1160nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
endodeoxyribonuclease activity21
Annotation ExtensionEvidenceWith/FromReference
endoribonuclease activity36
Annotation ExtensionEvidenceWith/FromReference
metal ion binding745
Annotation ExtensionEvidenceWith/FromReference
nuclease activity117
Annotation ExtensionEvidenceWith/FromReference
single-stranded DNA endodeoxyribonuclease activity7
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process

GO Slim Terms

DNA recombination


Term NameCount
cellular response to nitrogen starvation50
Annotation ExtensionEvidenceWith/FromReference
DNA catabolic process5
Annotation ExtensionEvidenceWith/FromReference
DNA catabolic process, endonucleolytic4
Annotation ExtensionEvidenceWith/FromReference
DNA recombination116
Annotation ExtensionEvidenceWith/FromReference
RNA catabolic process91
Annotation ExtensionEvidenceWith/FromReference
RNA phosphodiester bond hydrolysis, endonucleolytic26
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mitochondrion761
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
normal growth during cellular response to UVend1-379Not specified52
end1-458Not specified
viable vegetative cell populationpnu1ΔNull3795

Cell Phenotype

Term NameAlleleExpressionCount
decreased endodeoxyribonuclease activityend1-379Not specified3
end1-458Not specified
DNA fragmentationpnu1+ (wild type)Overexpression21
normal sporulationend1-379Not specified52
end1-458Not specified
viable cell at high temperatureend1-379Not specified8
end1-458Not specified
viable vegetative cellend1-379Not specified3630
end1-458Not specified
viable vegetative cell with normal cell morphologypnu1ΔNull3093
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4486171..4485713, 4485596..4485350, 4485274..4484695
Introns4485712..4485597, 4485349..4485275
mRNA4486171..4484695
5' UTR4486171..4485995PMID:21511999
CDS4485994..4485713, 4485596..4485350, 4485274..4484835
3' UTR4484834..4484695PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01223 Pfam IPR001604 DNA/RNA non-specific endonuclease 79 290 1
SM00477 SMART IPR020821 Extracellular Endonuclease, subunit A 78 291 1
SM00892 SMART IPR001604 DNA/RNA non-specific endonuclease 77 291 1
TMhelix TMHMM 5 27 959
PS01070 Prosite Patterns IPR018524 DNA/RNA non-specific endonuclease, active site 139 147 1
PTHR13966 HMMPANTHER 54 311 1
1g8tA00 Gene3D IPR020821 Extracellular Endonuclease, subunit A 60 304 1
SSF54060 SuperFamily 62 310 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.13 Da
Charge 9.00
Isoelectric point 9.14
Molecular weight 36.43 kDa
Number of residues 322
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
80102during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
65674during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
68873during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
74451during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
79544during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4918.24during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
10393.77during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.79during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:18488015432
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in metazoa3430
conserved in vertebrates3405
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
positive genetic interaction withatg6beclin family protein, involved in autophagy Positive GeneticPMID:22681890
positive genetic interaction withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withcdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:22681890
negative genetic interaction withdsc5UBX domain containing protein required for Sre1 cleavage Negative GeneticPMID:22681890
negative genetic interaction withelp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgcn20AAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withres2MBF transcription factor complex subunit Res2 Negative GeneticPMID:22681890
positive genetic interaction withsty1MAP kinase Sty1 Positive GeneticPMID:22681890
positive genetic interaction withtom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withxap5xap-5-like protein Positive GeneticPMID:24957674
External References
Database Identifier Description
NBRP SPAC17C9.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17C9.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17C9.08 BioGRID Interaction Datasets
Expression Viewer SPAC17C9.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17C9.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17C9.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17C9.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17C9.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17C9.08 Transcriptome Viewer (Bähler Lab)
GEO SPAC17C9.08 GEO profiles
PInt SPAC17C9.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC17C9.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC17C9.08 Fission yeast phenotypic data & analysis
Cyclebase SPAC17C9.08.1 Cell Cycle Data
SPD / RIKEN44/44A10Orfeome Localization Data
IntEnz3.1.30.-Integrated relational Enzyme database
Rhea3.1.30.-Annotated reactions database
UniProtKB/SwissProtQ10480Nuclease 1, mitochondrial
ModBaseQ10480Database of comparative protein structure models
STRINGQ10480Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594598mitochondrial endodeoxyribonuclease Pnu1
RefSeq mRNANM_001020026972h- mitochondrial endodeoxyribonuclease Pnu1 (pnu1), mRNA
European Nucleotide ArchiveAB050780ENA EMBL mapping
European Nucleotide ArchiveCAA97354.2ENA Protein Mapping
UniParcUPI00001687D6UniProt Archive

Literature for pnu1

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015