pnu1 (SPAC17C9.08)


Gene Standard Namepnu1 Characterisation Statuspublished
Systematic IDSPAC17C9.08 Feature Typeprotein coding
Synonymsend1, nuc1 Name Description
Productmitochondrial endodeoxyribonuclease Pnu1 Product Size322aa, 36.43 kDa
Genomic Location Chromosome I, 4486171-4484695 (1477nt); CDS:4485994-4484835 (1160nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004520endodeoxyribonuclease activityIMPPMID:609012219
GO:0004521endoribonuclease activityIDAPMID:1140627935
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037748
GO:0004518nuclease activityIMPPMID:17192844118
GO:0000014single-stranded DNA endodeoxyribonuclease activityIDAPMID:114062794
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006308DNA catabolic processISSUniProtKB:P08466GO_REF:000000122
GO:0000737DNA catabolic process, endonucleolyticIDAPMID:1140627912
IMPPMID:18047809
GO:0006310DNA recombinationISSUniProtKB:P08466GO_REF:0000001107
GO:0006401RNA catabolic processIMPPMID:1187216888
GO:0090502RNA phosphodiester bond hydrolysis, endonucleolyticIDAPMID:1140627924
GO:0006995cellular response to nitrogen starvationIMPPMID:1187216885
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0016020membraneIEAUniProtKB-KW:KW-0472GO_REF:00000371450
GO:0005739mitochondrionIDAPMID:11406279758
IDAPMID:11872168
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000969normal growth during cellular response to UVCell growth assayend1-379Not specifiedPMID:609012226
Cell growth assayend1-458Not specifiedPMID:6090122
FYPO:0002060viable vegetative cell populationMicroscopypnu1ΔNullPECO:0000005, PECO:0000137PMID:236978063751
Microscopypnu1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000160DNA fragmentationOthernot recorded (wild type)OverexpressionPMID:1804780920
FYPO:0002037decreased endodeoxyribonuclease activityEnzyme assay dataend1-458Not specifiedPECO:0000214PMID:60901221
Enzyme assay dataend1-379Not specifiedPECO:0000214PMID:6090122
FYPO:0000590normal sporulationCell growth assayend1-379Not specifiedPMID:609012245
Cell growth assayend1-458Not specifiedPMID:6090122
FYPO:0000282viable cell at high temperatureCell growth assayend1-458Not specifiedPMID:60901228
Cell growth assayend1-379Not specifiedPMID:6090122
FYPO:0001491viable vegetative cellCell growth assayend1-379Not specifiedPECO:0000006PMID:60901223599
Cell growth assayend1-458Not specifiedPECO:0000006PMID:6090122
Cell growth assayend1-379Not specifiedPECO:0000005PMID:6090122
Cell growth assayend1-458Not specifiedPECO:0000005PMID:6090122
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopypnu1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
144861714485713
244855964485350
344852744484695
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01223 Pfam IPR001604 DNA/RNA non-specific endonuclease 79 290 1
SM00477 SMART IPR020821 Extracellular Endonuclease, subunit A 78 291 1
SM00892 SMART IPR001604 DNA/RNA non-specific endonuclease 77 291 1
TMhelix TMHMM 5 27 959
PS01070 Prosite Patterns IPR018524 DNA/RNA non-specific endonuclease, active site 139 147 1
PTHR13966 HMMPANTHER 9 310 1
1g8tA00 Gene3D IPR020821 Extracellular Endonuclease, subunit A 60 304 1
SSF54060 SuperFamily 62 310 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.13 Da
Charge 9.00
Isoelectric point 9.14
Molecular weight 36.43 kDa
Number of residues 322
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
80102during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
65674during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
68873during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
74451during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
79544during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
4918.24during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
10393.77during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.79during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:18488015432
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in bacteria1002
conserved in metazoa3428
conserved in vertebrates3402
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
elp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
dsc5UBX domain containing protein required for Sre1 cleavage Negative GeneticPMID:22681890
ppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
atp11F1-ATPase chaperone Atp11 (predicted) Positive GeneticPMID:22681890
tom70mitochondrial TOM complex subunit Tom70 (predicted) Positive GeneticPMID:22681890
sty1MAP kinase Sty1 Positive GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
atg6beclin family protein, involved in autophagy Positive GeneticPMID:22681890
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
ask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
xap5xap-5-like protein Positive GeneticPMID:24957674
SPBC29A3.09cAAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
res2MBF transcription factor complex subunit Res2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC17C9.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17C9.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17C9.08 BioGRID Interaction Datasets
Expression Viewer SPAC17C9.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17C9.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17C9.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17C9.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17C9.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17C9.08 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC17C9.08 Cell Cycle Data
GEO SPAC17C9.08 GEO profiles
PInt SPAC17C9.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC17C9.08 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN44/44A10Orfeome Localization Data
IntEnz3.1.30.-Integrated relational Enzyme database
Rhea3.1.30.-Annotated reactions database
UniProtKB/SwissProtQ10480Nuclease 1, mitochondrial
ModBaseQ10480Database of comparative protein structure models
STRINGQ10480Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594598mitochondrial endodeoxyribonuclease Pnu1
RefSeq mRNANM_001020026972h- mitochondrial endodeoxyribonuclease Pnu1 (pnu1), mRNA
European Nucleotide ArchiveAB050780ENA EMBL mapping
European Nucleotide ArchiveCAA97354.2ENA Protein Mapping
UniParcUPI00001687D6UniProt Archive

Literature for pnu1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014