bro1 (SPAC17G6.05c)


Gene Standard Namebro1 Characterisation Statusbiological_role_inferred
Systematic IDSPAC17G6.05c Feature Typeprotein coding
Synonyms Name Description
ProductBRO1 domain protein Bro1 (predicted) Product Size775aa, 86.62 kDa
Genomic Location Chromosome I, 3598863-3596387 (2477nt); CDS:3598799-3596472 (2328nt)

Ensembl Gene Location
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0043328protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathwayISOSGD:S000006005GO_REF:000002415
GO:0006511ubiquitin-dependent protein catabolic processISOSGD:S000006005GO_REF:0000024170
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISOSGD:S000006005GO_REF:00000244198
GO:0005768endosomeISOSGD:S000006005GO_REF:000002477
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopybro1ΔNullPMID:204732893755
Microscopybro1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopybro1ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
135988633596387

UTRs

Region Start End Reference
three_prime_UTR35964713596387PMID:21511999
five_prime_UTR35988633598800PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF13949 Pfam IPR025304 ALIX V-shaped domain 437 748 2
PF03097 Pfam IPR004328 BRO1 domain 7 403 2
SM01041 SMART IPR004328 BRO1 domain 7 404 2
PS51180 Prosite Profiles IPR004328 BRO1 domain 7 414 2
PTHR23030 HMMPANTHER 1 775 2
PTHR23030:SF2 HMMPANTHER 1 775 1
G3DSA:1.25.40.280 Gene3D IPR004328 5 377 2
G3DSA:1.20.140.50 Gene3D 689 736 1
G3DSA:1.20.140.50 Gene3D 439 559 1
G3DSA:1.20.120.560 Gene3D 581 676 1
G3DSA:1.20.120.560 Gene3D 381 427 1
Coil ncoils Rabaptin coiled-coil domain 576 597 975
Coil ncoils Rabaptin coiled-coil domain 473 494 975
Low complexity (SEG) seg 754 768

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.77 Da
Charge -3.50
Isoelectric point 6.11
Molecular weight 86.62 kDa
Number of residues 775
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1903.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
4328.36during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.87during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
2.1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
dbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
SPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Negative GeneticPMID:22681890
vps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
vps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
SPCC1223.01ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
ssm4dynactin microtubule-binding subunit, p150-Glued Ssm4 Negative GeneticPMID:22681890
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
aut12autophagy associated protein Aut12 (predicted) Negative GeneticPMID:22681890
sep1forkhead transcription factor Sep1 Negative GeneticPMID:22681890
spc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Positive GeneticPMID:22681890
sif1Sad1 interacting factor 1 Positive GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPAC17G6.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17G6.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17G6.05c BioGRID Interaction Datasets
Expression Viewer SPAC17G6.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17G6.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17G6.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17G6.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17G6.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17G6.05c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC17G6.05c Cell Cycle Data
GEO SPAC17G6.05c GEO profiles
PInt SPAC17G6.05c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542419vacuolar protein-sorting protein
EntrezGene2542419vacuolar protein-sorting protein
SPD / RIKEN28/28D03Orfeome Localization Data
UniProtKB/SwissProtO13783Vacuolar protein-sorting protein bro1
ModBaseO13783Database of comparative protein structure models
StringO13783Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594252vacuolar protein-sorting protein
RefSeq mRNANM_001019675972h- vacuolar protein-sorting protein (SPAC17G6.05c), mRNA
European Nucleotide ArchiveCAB16216ENA Protein Mapping
UniParcUPI00000695D4UniProt Archive

Literature for bro1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014