bro1 (SPAC17G6.05c)


Gene Standard Namebro1 Characterisation Statusbiological role inferred
Systematic IDSPAC17G6.05c Feature Typeprotein coding
Synonyms Name Description
ProductBRO1 domain protein Bro1 (predicted) Product Size775aa, 86.62 kDa
Genomic Location Chromosome I, 3598863-3596387 (2477nt); CDS:3598799-3596472 (2328nt)

Ensembl Gene Location
GO Biological Process
GO Cellular Component
Term NameCount
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
endosome80
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
resistance to tamoxifenbro1Δ61
viable vegetative cell populationbro1Δ3837

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologybro1Δ3100
Target Of
OntologyRelationshipGeneProduct
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3598863..3596387
5' UTR3598863..3598800PMID:21511999
3' UTR3596471..3596387PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03097 Pfam IPR004328 BRO1 domain 10 399 2
PF13949 Pfam IPR025304 ALIX V-shaped domain 453 746 1
SM01041 SMART IPR004328 BRO1 domain 7 404 2
PS51180 Prosite Profiles IPR004328 BRO1 domain 7 414 2
PTHR23030 HMMPANTHER 1 772 2
PTHR23030:SF13 HMMPANTHER 1 772 1
2xs1A03 Gene3D 689 736 1
2xs1A03 Gene3D 439 559 1
2xs1A02 Gene3D 381 427 1
2xs1A02 Gene3D 581 676 1
2xs1A01 Gene3D IPR004328 BRO1 domain 5 377 2
Coil ncoils Predicted coiled-coil protein (DUF2205) 473 493 1057
Coil ncoils Predicted coiled-coil protein (DUF2205) 576 596 1057

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.77 Da
Charge -3.50
Codon Adaptation Index 0.44
Isoelectric point 6.11
Molecular weight 86.62 kDa
Number of residues 775
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for bro1 (SPAC17G6.05c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
17311during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
17488during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
16844during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
17761during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4328.36during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
18093during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1903.96during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.1during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.87during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4605
conserved in eukaryotes4515
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC17G6.05c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:22681890
negative genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
negative genetic interaction withSPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
negative genetic interaction withvps17retromer complex subunit Vps17 Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:22681890
negative genetic interaction withsep1forkhead transcription factor Sep1 Negative GeneticPMID:22681890
negative genetic interaction withssm4dynactin microtubule-binding subunit, p150-Glued Ssm4 Negative GeneticPMID:22681890
negative genetic interaction withaut12autophagy associated protein Aut12 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withspc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
negative genetic interaction withvps5retromer complex subunit Vps5 Negative GeneticPMID:22681890
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withsif1Sad1 interacting factor 1 Positive GeneticPMID:22681890
positive genetic interaction withgcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Positive GeneticPMID:22681890
positive genetic interaction withhus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
positive genetic interaction withSPCC1223.01ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC17G6.05c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17G6.05c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17G6.05c BioGRID Interaction Datasets
Expression Viewer SPAC17G6.05c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17G6.05c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17G6.05c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17G6.05c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17G6.05c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17G6.05c Transcriptome Viewer (Bähler Lab)
GEO SPAC17G6.05c GEO profiles
PInt SPAC17G6.05c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC17G6.05c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC17G6.05c Fission yeast phenotypic data & analysis
Cyclebase SPAC17G6.05c.1 Cell Cycle Data
SPD / RIKEN28/28D03Orfeome Localization Data
UniProtKB/SwissProtO13783Vacuolar protein-sorting protein bro1
ModBaseO13783Database of comparative protein structure models
STRINGO13783Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594252vacuolar protein-sorting protein
RefSeq mRNANM_001019675972h- vacuolar protein-sorting protein (SPAC17G6.05c), mRNA

Literature for bro1

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016