pep7 (SPAC17G6.08)


Gene Standard Namepep7 Characterisation Statusbiological role inferred
Systematic IDSPAC17G6.08 Feature Typeprotein coding
Synonymsvac1 Name Description
Productprevacuole/endosomal FYVE tethering component Pep7 (predicted) Product Size536aa, 62.43 kDa
Genomic Location Chromosome I, 3601674-3603904 (2231nt); CDS:3601879-3603489 (1611nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
metal ion binding749
Annotation ExtensionEvidenceWith/FromReference
phosphatidylinositol-3-phosphate binding12
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
Golgi to vacuole transport29
Annotation ExtensionEvidenceWith/FromReference
vesicle docking involved in exocytosis10
Annotation ExtensionEvidenceWith/FromReference
vesicle fusion9
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4212
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus357
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencypep7Δ272
loss of viability in stationary phasepep7Δ61
resistance to brefeldin Apep7Δ19
resistance to caffeine and rapamycinpep7Δ33
viable vegetative cell populationpep7Δ3844

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologypep7Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3601674..3603904
mRNA3601674..3603904
5' UTR3601674..3601878PMID:21511999
CDS3601879..3603489
3' UTR3603490..3603904PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01363 Pfam IPR000306 FYVE zinc finger 271 331 5
PF01363 Pfam IPR000306 FYVE zinc finger 130 198 5
PF11464 Pfam IPR021565 Rabenosyn, Rab binding domain 483 523 1
SM00064 SMART IPR000306 FYVE zinc finger 267 333 6
SM00064 SMART IPR000306 FYVE zinc finger 126 202 6
PS50178 Prosite Profiles IPR017455 Zinc finger, FYVE-related 275 332 5
PS50178 Prosite Profiles IPR017455 Zinc finger, FYVE-related 145 201 5
PS00028 Prosite Patterns IPR007087 Zinc finger, C2H2 41 62 28
PTHR13510:SF21 HMMPANTHER 182 536 1
PTHR13510 HMMPANTHER 182 536 1
3.30.40.10 Gene3D IPR013083 Zinc finger, RING/FYVE/PHD-type 267 331 86
3.30.40.10 Gene3D IPR013083 Zinc finger, RING/FYVE/PHD-type 128 198 86
SSF140125 SuperFamily 477 522 1
SSF57903 SuperFamily IPR011011 Zinc finger, FYVE/PHD-type 129 218 25
SSF57903 SuperFamily IPR011011 Zinc finger, FYVE/PHD-type 253 331 25
Coil ncoils Predicted coiled-coil protein (DUF2205) 456 487 1057

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000137zinc finger proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000137
PBO:0000153zf-C2H2 typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000153
PBO:0001569zf-FYVE typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001569

Protein Properties

Ave. residue weight 116.48 Da
Charge 14.00
Codon Adaptation Index 0.44
Isoelectric point 7.97
Molecular weight 62.43 kDa
Number of residues 536
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1677
present during mitotic M phaseS75
present during mitotic M phaseS78
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S75 PMID:21712547
present during mitotic M phase experimental evidence S78 PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for pep7 (SPAC17G6.08)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
857.6during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.84during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.31during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC17G6.08 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withvps45vacuolar sorting protein Vps45 Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC17G6.08 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:22681890
negative genetic interaction withshg1Set1C complex subunit Shg1 Negative GeneticPMID:22681890
negative genetic interaction withaut12autophagy associated protein Aut12 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withdsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:22681890
negative genetic interaction withdsc4Golgi Dsc E3 ligase complex subunit Dsc4 Negative GeneticPMID:21504829
positive genetic interaction withcdc18MCM loader Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC17G6.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17G6.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17G6.08 BioGRID Interaction Datasets
Expression Viewer SPAC17G6.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17G6.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17G6.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17G6.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17G6.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17G6.08 Transcriptome Viewer (Bähler Lab)
GEO SPAC17G6.08 GEO profiles
PInt SPAC17G6.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC17G6.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC17G6.08 Fission yeast phenotypic data & analysis
Cyclebase SPAC17G6.08.1 Cell Cycle Data
SPD / RIKEN25/25H01Orfeome Localization Data
UniProtKB/SwissProtO13786Vacuolar segregation protein pep7
ModBaseO13786Database of comparative protein structure models
STRINGO13786Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594255prevacuole/endosomal FYVE tethering component Pep7 (predicted)
RefSeq mRNANM_001019678972h- prevacuole/endosomal FYVE tethering component Pep7 (predicted) (pep7), mRNA
European Nucleotide ArchiveCAB16219.1ENA Protein Mapping
UniParcUPI000006AA47UniProt Archive

Literature for pep7

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016