pep7 (SPAC17G6.08)


Gene Standard Namepep7 Characterisation Statusbiological_role_inferred
Systematic IDSPAC17G6.08 Feature Typeprotein coding
Synonymsvac1 Name Description
Productprevacuole/endosomal FYVE tethering component Pep7 (predicted) Product Size536aa, 62.43 kDa
Genomic Location Chromosome I, 3601674-3603904 (2231nt); CDS:3601879-3603489 (1611nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
metal ion binding747
Annotation ExtensionEvidenceWith/FromReference
phosphatidylinositol-3-phosphate binding13
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
Golgi to vacuole transport18
Annotation ExtensionEvidenceWith/FromReference
vesicle docking involved in exocytosis10
Annotation ExtensionEvidenceWith/FromReference
vesicle fusion10
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4205
Annotation ExtensionEvidenceWith/FromReference
Golgi apparatus355
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000245loss of viability in stationary phaseCell growth assaypep7ΔNullPECO:0000137PMID:2446336541
FYPO:0000067resistance to brefeldin ACell growth assaypep7ΔNullPECO:0000137PMID:2446336519
FYPO:0003824resistance to caffeine and rapamycinCell growth assaypep7ΔNullPECO:0000137PMID:2446336533
FYPO:0002060viable vegetative cell populationMicroscopypep7ΔNullPECO:0000005, PECO:0000137PMID:236978063759
Microscopypep7ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidencepep7ΔNullPECO:0000015PMID:23950735240
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopypep7ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
136016743603904

UTRs

Region Coordinates Reference
five_prime_UTR3601674..3601878PMID:21511999
three_prime_UTR3603490..3603904PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01363 Pfam IPR000306 FYVE zinc finger 130 198 5
PF01363 Pfam IPR000306 FYVE zinc finger 271 331 5
SM00064 SMART IPR000306 FYVE zinc finger 126 202 6
SM00064 SMART IPR000306 FYVE zinc finger 267 333 6
PS50178 Prosite Profiles IPR017455 Zinc finger, FYVE-related 275 332 5
PS50178 Prosite Profiles IPR017455 Zinc finger, FYVE-related 145 201 5
PS00028 Prosite Patterns IPR007087 Zinc finger, C2H2 41 62 28
PTHR13510 HMMPANTHER 1 536 1
PTHR13510:SF9 HMMPANTHER 1 536 1
3.30.40.10 Gene3D IPR013083 Zinc finger, RING/FYVE/PHD-type 267 331 86
3.30.40.10 Gene3D IPR013083 Zinc finger, RING/FYVE/PHD-type 128 198 86
SSF57903 SuperFamily IPR011011 Zinc finger, FYVE/PHD-type 253 331 25
SSF140125 SuperFamily 477 522 1
SSF57903 SuperFamily IPR011011 Zinc finger, FYVE/PHD-type 129 218 25
Coil ncoils Rabaptin coiled-coil domain 456 488 968

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000137zinc finger proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000137
PBO:0000153zf-C2H2 typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000153
PBO:0001569zf-FYVE typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001569

Protein Properties

Ave. residue weight 116.48 Da
Charge 14.00
Isoelectric point 7.97
Molecular weight 62.43 kDa
Number of residues 536
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS78PMID:217125471670
present during mitotic M phase
present during mitotic M phaseexperimental evidenceS75PMID:21712547
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
857.6during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.84during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.31during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
shg1Set1C complex subunit Shg1 Negative GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
cdc18MCM loader Positive GeneticPMID:22681890
aut12autophagy associated protein Aut12 (predicted) Negative GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC17G6.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17G6.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17G6.08 BioGRID Interaction Datasets
Expression Viewer SPAC17G6.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17G6.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17G6.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17G6.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17G6.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17G6.08 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC17G6.08 Cell Cycle Data
GEO SPAC17G6.08 GEO profiles
PInt SPAC17G6.08 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC17G6.08 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC17G6.08 Fission yeast phenotypic data & analysis
SPD / RIKEN25/25H01Orfeome Localization Data
UniProtKB/SwissProtO13786Vacuolar segregation protein pep7
ModBaseO13786Database of comparative protein structure models
STRINGO13786Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594255prevacuole/endosomal FYVE tethering component Pep7 (predicted)
RefSeq mRNANM_001019678972h- prevacuole/endosomal FYVE tethering component Pep7 (predicted) (pep7), mRNA
European Nucleotide ArchiveCAB16219.1ENA Protein Mapping
UniParcUPI000006AA47UniProt Archive

Literature for pep7

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014