pep7 (SPAC17G6.08)


Gene Standard Namepep7 Characterisation Statusbiological_role_inferred
Systematic IDSPAC17G6.08 Feature Typeprotein coding
Synonymsvac1 Name Description
Productprevacuole/endosomal FYVE tethering component Pep7 (predicted) Product Size536aa, 62.43 kDa
Genomic Location Chromosome I, 3601674-3603904 (2231nt); CDS:3601879-3603489 (1611nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0046872metal ion bindingIEAUniProtKB-KW:KW-0479GO_REF:0000037747
GO:0032266phosphatidylinositol-3-phosphate bindingISOSGD:S000002731GO_REF:000002412
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006896Golgi to vacuole transportISOSGD:S000002731GO_REF:000002418
GO:0006904vesicle docking involved in exocytosisISOSGD:S000002731GO_REF:00000249
GO:0006906vesicle fusionISOSGD:S000002731GO_REF:000002411
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005794Golgi apparatusIDAPMID:16823372355
GO:0005737cytoplasmIDAPMID:168233724198
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopypep7ΔNullPMID:204732893755
Microscopypep7ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidencepep7ΔNullPECO:0000015PMID:23950735222
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopypep7ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
136016743603904

UTRs

Region Start End Reference
three_prime_UTR36034903603904PMID:21511999
five_prime_UTR36016743601878PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01363 Pfam IPR000306 FYVE zinc finger 130 198 5
PF01363 Pfam IPR000306 FYVE zinc finger 271 331 5
SM00064 SMART IPR000306 FYVE zinc finger 126 202 6
SM00064 SMART IPR000306 FYVE zinc finger 267 333 6
SM00355 SMART IPR015880 Zinc finger, C2H2-like 156 179 29
SM00355 SMART IPR015880 Zinc finger, C2H2-like 39 62 29
PS00028 Prosite Patterns IPR007087 Zinc finger, C2H2 41 62 28
PS50178 Prosite Profiles IPR017455 Zinc finger, FYVE-related 275 332 5
PS50178 Prosite Profiles IPR017455 Zinc finger, FYVE-related 145 201 5
PTHR13510:SF9 HMMPANTHER 1 536 1
PTHR13510 HMMPANTHER 1 536 1
G3DSA:3.30.40.10 Gene3D IPR013083 128 198 84
G3DSA:3.30.40.10 Gene3D IPR013083 267 331 84
SSF57903 SuperFamily IPR011011 129 218 25
SSF57903 SuperFamily IPR011011 253 331 25
SSF140125 SuperFamily 477 522 1
Coil ncoils Rabaptin coiled-coil domain 456 488 975
Low complexity (SEG) seg 457 477
Low complexity (SEG) seg 289 302

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000137zinc finger proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000137
PBO:0000153zf-C2H2 typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000153
PBO:0001569zf-FYVE typeTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001569

Protein Properties

Ave. residue weight 116.48 Da
Charge 14.00
Isoelectric point 7.97
Molecular weight 62.43 kDa
Number of residues 536
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
857.6during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.84during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.31during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
dsc4Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
shg1Set1C complex subunit Shg1 Negative GeneticPMID:22681890
dsc1Golgi Dsc E3 ligase complex subunit Dsc1 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
cdc18MCM loader Positive GeneticPMID:22681890
aut12autophagy associated protein Aut12 (predicted) Negative GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC17G6.08 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17G6.08 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17G6.08 BioGRID Interaction Datasets
Expression Viewer SPAC17G6.08 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17G6.08 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17G6.08 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17G6.08 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17G6.08 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17G6.08 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC17G6.08 Cell Cycle Data
GEO SPAC17G6.08 GEO profiles
PInt SPAC17G6.08 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542423prevacuole/endosomal FYVE tethering component Pep7 (predicted)
EntrezGene2542423prevacuole/endosomal FYVE tethering component Pep7 (predicted)
SPD / RIKEN25/25H01Orfeome Localization Data
UniProtKB/SwissProtO13786Vacuolar segregation protein pep7
ModBaseO13786Database of comparative protein structure models
StringO13786Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594255prevacuole/endosomal FYVE tethering component Pep7 (predicted)
RefSeq mRNANM_001019678972h- prevacuole/endosomal FYVE tethering component Pep7 (predicted) (pep7), mRNA
European Nucleotide ArchiveCAB16219ENA Protein Mapping
UniParcUPI000006AA47UniProt Archive

Literature for pep7

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014