cul1 (SPAC17G6.12)


Gene Standard Namecul1 Characterisation Statuspublished
Systematic IDSPAC17G6.12 Feature Typeprotein coding
Synonymspcu1 Name Description
Productcullin 1 Product Size767aa, 89.43 kDa
Genomic Location Chromosome I, 3612082-3614883 (2802nt); CDS:3612124-3614883 (2760nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031625ubiquitin protein ligase bindingIEAIPR001373GO_REF:00000026
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032876negative regulation of DNA endoreduplicationIMPPMID:99905073
GO:0042787protein ubiquitination involved in ubiquitin-dependent protein catabolic processICGO:0019005GO_REF:000000113
GO:0007346regulation of mitotic cell cycleICGO:0019005GO_REF:0000001281
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0019005SCF ubiquitin ligase complexIDAPMID:108804609
GO:0005829cytosolIDAPMID:168233722316
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopycul1ΔNullPMID:204732891331
Microscopycul1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopycul1ΔNullPECO:0000005, PECO:0000137PMID:23697806638
FYPO:0000216abnormal negative regulation of DNA replication initiationcul1ΔNullPMID:99905077
FYPO:0001122elongated vegetative cellMicroscopycul1ΔNullPMID:9990507657
FYPO:0002262inviable after spore germination, multiple cell divisions, elongated cell94
penetrance FYPO_EXT:0000001Microscopycul1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
GO input for ned8 ubiquitin-like protein modifier Ned8 PMID:10880460
GO substrate of nep1 NEDD8 protease Nep1 PMID:15769255
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
136120823612191
236122373612323
336124223612486
436125983612674
536128773614883

UTRs

Region Coordinates Reference
five_prime_UTR3612082..3612123PMID:18641648
five_prime_UTR3612082..3612123PMID:20118936
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00888 Pfam IPR001373 Cullin, N-terminal 24 669 4
PF10557 Pfam IPR019559 Cullin protein, neddylation domain 696 760 3
SM00182 SMART IPR016158 Cullin homology 442 589 4
SM00884 SMART IPR019559 Cullin protein, neddylation domain 696 763 3
PS50069 Prosite Profiles IPR016158 Cullin homology 412 642 4
PS01256 Prosite Patterns IPR016157 Cullin, conserved site 742 767 3
PTHR11932:SF19 HMMPANTHER 1 767 1
PTHR11932 HMMPANTHER 1 767 4
1.20.1310.10 Gene3D 21 168 3
1ldjA05 Gene3D 525 593 4
1.20.1310.10 Gene3D 169 294 3
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 667 767 46
1.20.1310.10 Gene3D 416 519 3
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 602 666 46
1.20.1310.10 Gene3D 295 415 3
SSF46785 SuperFamily 681 767 49
SSF75632 SuperFamily IPR016158 Cullin homology 409 678 4
SSF74788 SuperFamily IPR016159 Cullin repeat-like-containing domain 22 406 6
Coil ncoils Rabaptin coiled-coil domain 254 275 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.59 Da
Charge 1.00
Isoelectric point 6.61
Molecular weight 89.43 kDa
Number of residues 767
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:01150neddylated lysineNASPMID:108804602
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
7550during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
8531during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
8555during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
7817during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
8955during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
2902.52during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
5886.17during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.5during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.69during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
gga21Golgi localized Arf binding gamma-adaptin ortholog Gga21 Negative GeneticPMID:22681890
SPAC1F12.04cconserved fungal protein Negative GeneticPMID:22681890
SPAC926.06cleucine-rich repeat protein, unknown Negative GeneticPMID:22681890
fas1fatty acid synthase beta subunit Fas1 Negative GeneticPMID:22681890
rpp20360S acidic ribosomal protein A2 Negative GeneticPMID:22681890
SPAC1071.03csequence orphan Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
nep2NEDD8 protease Nep2 Reconstituted ComplexPMID:15769255
ned8ubiquitin-like protein modifier Ned8 Affinity Capture-WesternPMID:11854407
Affinity Capture-WesternPMID:10880460
rbx1RING-box protein 1, SCF complex ubiquitin ligase subunit Affinity Capture-WesternPMID:23535663
Affinity Capture-MS
Affinity Capture-WesternPMID:12167173
pof14F-box protein Pof14 Affinity Capture-MSPMID:23535663
Affinity Capture-WesternPMID:17016471
skp1SCF ubiquitin ligase complex subunit Skp1 Affinity Capture-WesternPMID:15147268
Affinity Capture-MSPMID:23535663
Affinity Capture-WesternPMID:12167173
Affinity Capture-WesternPMID:15555586
pof9F-box protein Pof9 Affinity Capture-MSPMID:23535663
Affinity Capture-WesternPMID:19748355
csn5COP9/signalosome complex subunit Csn5 Affinity Capture-MSPMID:23535663
Biochemical ActivityPMID:12183637
Affinity Capture-Western
csn1COP9/signalosome complex subunit Csn1 Affinity Capture-MSPMID:23535663
Affinity Capture-WesternPMID:12183637
pof3F-box protein Pof3 Affinity Capture-WesternPMID:11809834
Affinity Capture-WesternPMID:19748355
pof7F-box protein Pof7 Affinity Capture-MSPMID:23535663
Affinity Capture-WesternPMID:19748355
csn4COP9/signalosome complex subunit Csn4 Affinity Capture-MSPMID:23535663
pof5F-box protein Pof5 Affinity Capture-MSPMID:23535663
pof10F-box protein Pof10 Affinity Capture-WesternPMID:23535663
Affinity Capture-MS
Affinity Capture-WesternPMID:19748355
pop2F-box/WD repeat protein Pop2 Affinity Capture-MSPMID:23535663
Affinity Capture-WesternPMID:9990507
Affinity Capture-WesternPMID:12167173
pop1cullin 1 adaptor protein Pop1 Affinity Capture-WesternPMID:10880460
Affinity Capture-WesternPMID:12167173
Affinity Capture-MSPMID:23535663
pof1F-box/WD repeat protein protein Pof1 Affinity Capture-WesternPMID:15660136
Affinity Capture-WesternPMID:23535663
Affinity Capture-MS
Affinity Capture-WesternPMID:19748355
csn2COP9/signalosome complex subunit Csn2 Affinity Capture-MSPMID:23535663
nep1NEDD8 protease Nep1 Reconstituted ComplexPMID:15769255
cul1cullin 1 Affinity Capture-MSPMID:23535663
pof11F-box protein Pof11 Affinity Capture-MSPMID:23535663
External References
Database Identifier Description
NBRP SPAC17G6.12 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17G6.12 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17G6.12 BioGRID Interaction Datasets
Expression Viewer SPAC17G6.12 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17G6.12 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17G6.12 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17G6.12 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17G6.12 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17G6.12 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC17G6.12 Cell Cycle Data
GEO SPAC17G6.12 GEO profiles
PInt SPAC17G6.12 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC17G6.12 Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2542393cullin 1
EntrezGene2542393cullin 1
SPD / RIKEN47/47B10Orfeome Localization Data
UniProtKB/SwissProtO13790Cullin-1
ModBaseO13790Database of comparative protein structure models
STRINGO13790Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594259cullin 1
RefSeq mRNANM_001019682972h- cullin 1 (cul1), mRNA
European Nucleotide ArchiveBAA32428ENA Protein Mapping
European Nucleotide ArchiveBAA32428.2ENA Protein Mapping
European Nucleotide ArchiveBAA87201ENA Protein Mapping
European Nucleotide ArchiveBAA87201.1ENA Protein Mapping
European Nucleotide ArchiveCAB16223ENA Protein Mapping
European Nucleotide ArchiveCAB16223.1ENA Protein Mapping
ReactomeREACT_208775
UniParcUPI00001286E7UniProt Archive

Literature for cul1

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014