cul1 (SPAC17G6.12)


Gene Standard Namecul1 Characterisation Statuspublished
Systematic IDSPAC17G6.12 Feature Typeprotein coding
Synonyms Name Description
Productcullin 1 Product Size767aa, 89.43 kDa
Genomic Location Chromosome I, 3612082-3614883 (2802nt); CDS:3612124-3614883 (2760nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
protein binding862
Annotation ExtensionEvidenceWith/FromReference
ubiquitin protein ligase binding4
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
nuclear SCF ubiquitin ligase complex5
Annotation ExtensionEvidenceWith/FromReference
SCF ubiquitin ligase complex11
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationcul1Δ1450

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cyclecul1Δ849
abnormal negative regulation of mitotic DNA replication initiationcul1Δ10
elongated vegetative cellcul1Δ787
inviable after spore germination, multiple cell divisions, cell cycle arrest in mitotic interphase, elongated cellscul1Δ88
Target Of
OntologyRelationshipGeneProduct
GO substrate of nep1 NEDD8 protease Nep1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons3612082..3612191, 3612237..3612323, 3612422..3612486, 3612598..3612674, 3612877..3614883
Intron3612192..3612236, 3612324..3612421, 3612487..3612597, 3612675..3612876
5' UTR3612082..3612123PMID:20118936
5' UTR3612082..3612123PMID:18641648
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00888 Pfam IPR001373 Cullin, N-terminal 24 668 4
PF10557 Pfam IPR019559 Cullin protein, neddylation domain 697 759 3
SM00182 SMART IPR016158 Cullin homology 442 589 4
SM00884 SMART IPR019559 Cullin protein, neddylation domain 696 763 3
PS01256 Prosite Patterns IPR016157 Cullin, conserved site 742 767 3
PS50069 Prosite Profiles IPR016158 Cullin homology 412 642 4
PTHR11932 HMMPANTHER 1 767 4
PTHR11932:SF81 HMMPANTHER 1 767 1
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 602 666 46
1.10.10.10 Gene3D IPR011991 Winged helix-turn-helix DNA-binding domain 667 767 46
1.20.1310.10 Gene3D Cullin, N-terminal 21 168 3
1.20.1310.10 Gene3D Cullin, N-terminal 169 294 3
1.20.1310.10 Gene3D Cullin, N-terminal 295 415 3
1.20.1310.10 Gene3D Cullin, N-terminal 416 519 3
1ldjA05 Gene3D 525 593 4
SSF46785 SuperFamily IPR011991 Winged helix-turn-helix DNA-binding domain 681 767 49
SSF74788 SuperFamily IPR016159 Cullin repeat-like-containing domain 22 406 6
SSF75632 SuperFamily IPR016158 Cullin homology 409 678 4
Coil ncoils Predicted coiled-coil protein (DUF2205) 254 274 1050

View domain organization at Pfam

Protein Properties

Ave. residue weight 116.59 Da
Charge 1.00
Codon Adaptation Index 0.43
Isoelectric point 6.61
Molecular weight 89.43 kDa
Number of residues 767
Modifications

Protein Modifications

Term NameResidueCount
neddylated lysine 2
Annotation ExtensionEvidenceResidueReference
IDA PMID:10880460
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for cul1 (SPAC17G6.12)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
7550during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8531during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
8555during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
7817during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2902.52during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
8955during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5886.17during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.5during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.69during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3422
conserved in vertebrates3397
conserved in eukaryotes only2504
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPAC17G6.12 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bypof1F-box/WD repeat protein protein Pof1 Affinity Capture-WesternPMID:15660136
affinity captured bypof10F-box protein Pof10 Affinity Capture-WesternPMID:19748355
affinity captured byskp1SCF ubiquitin ligase complex subunit Skp1 Affinity Capture-WesternPMID:15555586
affinity captured bypof7F-box protein Pof7 Affinity Capture-WesternPMID:19748355
affinity captured bypof14F-box protein Pof14 Affinity Capture-WesternPMID:17016471
affinity captured bypop2F-box/WD repeat protein Pop2 Affinity Capture-WesternPMID:12167173
affinity captured bypof3F-box protein Pof3 Affinity Capture-WesternPMID:19748355
affinity captured bypop1cullin 1 adaptor protein Pop1 Affinity Capture-WesternPMID:12167173
affinity captured bypof9F-box protein Pof9 Affinity Capture-WesternPMID:19748355
affinity captured byrbx1SCF complex and CLRC ubiquitin ligase ligase E3 subunit Rbx1 Affinity Capture-WesternPMID:12167173
affinity captured bycsn2COP9/signalosome complex subunit Csn2 Affinity Capture-WesternPMID:11337588
affinity capturespop1cullin 1 adaptor protein Pop1 Affinity Capture-MSPMID:23535663
affinity capturesskp1SCF ubiquitin ligase complex subunit Skp1 Affinity Capture-MSPMID:23535663
affinity capturespof3F-box protein Pof3 Affinity Capture-WesternPMID:11809834
affinity capturesrbx1SCF complex and CLRC ubiquitin ligase ligase E3 subunit Rbx1 Affinity Capture-WesternPMID:12167173
affinity capturespop2F-box/WD repeat protein Pop2 Affinity Capture-MSPMID:23535663
affinity capturescsn1COP9/signalosome complex subunit Csn1 Affinity Capture-WesternPMID:12183637
affinity capturespof9F-box protein Pof9 Affinity Capture-MSPMID:23535663
affinity capturespof14F-box protein Pof14 Affinity Capture-MSPMID:23535663
affinity capturescul1cullin 1 Affinity Capture-MSPMID:23535663
affinity capturescsn5COP9/signalosome complex subunit Csn5 Affinity Capture-WesternPMID:12183637
affinity capturespof10F-box protein Pof10 Affinity Capture-WesternPMID:23535663
affinity capturespof11F-box protein Pof11 Affinity Capture-MSPMID:23535663
affinity capturespof1F-box/WD repeat protein protein Pof1 Affinity Capture-MSPMID:23535663
affinity capturesned8ubiquitin-like protein modifier Ned8 Affinity Capture-WesternPMID:11854407
affinity capturespof7F-box protein Pof7 Affinity Capture-MSPMID:23535663
affinity capturespof5F-box protein Pof5 Affinity Capture-MSPMID:23535663
affinity capturescsn4COP9/signalosome complex subunit Csn4 Affinity Capture-MSPMID:23535663
affinity capturescsn2COP9/signalosome complex subunit Csn2 Affinity Capture-MSPMID:23535663
modified bycsn5COP9/signalosome complex subunit Csn5 Biochemical ActivityPMID:12183637
modifiescul1cullin 1 Biochemical ActivityPMID:11337588
forms complex withnep2NEDD8 protease Nep2 Reconstituted ComplexPMID:15769255
forms complex withubi4ubiquitin Reconstituted ComplexPMID:11337588
forms complex withnep1NEDD8 protease Nep1 Reconstituted ComplexPMID:15769255
Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPAC17G6.12 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withgga21Golgi localized Arf binding gamma-adaptin ortholog Gga21 Negative GeneticPMID:22681890
negative genetic interaction withSPAC1F12.04cconserved fungal protein Negative GeneticPMID:22681890
negative genetic interaction withSPAC926.06cleucine-rich repeat protein, unknown role Negative GeneticPMID:22681890
negative genetic interaction withrpp20360S acidic ribosomal protein A2 Negative GeneticPMID:22681890
negative genetic interaction withsil1nucleotide exchange factor for the ER lumenal Hsp70 chaperone, Sil1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withfas1fatty acid synthase beta subunit Fas1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC17G6.12 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC17G6.12 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC17G6.12 BioGRID Interaction Datasets
Expression Viewer SPAC17G6.12 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC17G6.12 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC17G6.12 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC17G6.12 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC17G6.12 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC17G6.12 Transcriptome Viewer (Bähler Lab)
GEO SPAC17G6.12 GEO profiles
PInt SPAC17G6.12 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC17G6.12 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC17G6.12 Fission yeast phenotypic data & analysis
Cyclebase SPAC17G6.12.1 Cell Cycle Data
SPD / RIKEN47/47B10Orfeome Localization Data
UniProtKB/SwissProtO13790Cullin-1
ModBaseO13790Database of comparative protein structure models
STRINGO13790Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594259cullin 1
RefSeq mRNANM_001019682972h- cullin 1 (cul1), mRNA
European Nucleotide ArchiveAB016896ENA EMBL mapping
European Nucleotide ArchiveAB027897ENA EMBL mapping
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveBAA32428ENA Protein Mapping
European Nucleotide ArchiveBAA87201ENA Protein Mapping
European Nucleotide ArchiveCAB16223ENA Protein Mapping
UniParcUPI00001286E7UniProt Archive

Literature for cul1

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016