hem2 (SPAC1805.06c)


Gene Standard Namehem2 Characterisation Statusbiological_role_inferred
Systematic IDSPAC1805.06c Feature Typeprotein coding
Synonyms Name Description
Productporphobilinogen synthase Hem2 (predicted) Product Size329aa, 36.12 kDa
Genomic Location Chromosome I, 2785086-2783547 (1540nt); CDS:2784930-2783941 (990nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032791lead ion bindingISSUniProtKB:P13716GO_REF:00000241
GO:0004655porphobilinogen synthase activityISSUniProtKB:P13716GO_REF:00000241
ISOSGD:S000003008GO_REF:0000024
GO:0008270zinc ion bindingISSUniProtKB:P13716GO_REF:0000024249
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006783heme biosynthetic processISSUniProtKB:P13716GO_REF:000002413
ISOSGD:S000003008GO_REF:0000024
GO:0006782protoporphyrinogen IX biosynthetic processIEA UniPathway:UPA00251GO_REF:00000417
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISOSGD:S000003008GO_REF:00000244204
GO:0005829cytosolIDAPMID:168233722319
GO:0005634nucleusISOSGD:S000003008GO_REF:00000242737
IDAPMID:16823372
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000088sensitive to hydroxyureaCell growth assayhem2ΔNullPECO:0000137, PECO:0000005PMID:23173672513
FYPO:0002060viable vegetative cell populationMicroscopyhem2ΔNullPMID:204732893751
Microscopyhem2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopyhem2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
127850862783547
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00490 Pfam IPR001731 Porphobilinogen synthase 10 327 1
SM01004 SMART IPR001731 Porphobilinogen synthase 4 327 1
PS00169 Prosite Patterns IPR001731 Porphobilinogen synthase 245 257 1
PTHR11458:SF0 HMMPANTHER 1 329 1
PTHR11458 HMMPANTHER IPR001731 Porphobilinogen synthase 1 329 1
3.20.20.70 Gene3D IPR013785 Aldolase-type TIM barrel 5 328 35
SSF51569 SuperFamily 9 328 9
PIRSF001415 PIRSF IPR001731 Porphobilinogen synthase 1 329 1
PR00144 PRINTS IPR001731 Porphobilinogen synthase 301 320 1
PR00144 PRINTS IPR001731 Porphobilinogen synthase 123 137 1
PR00144 PRINTS IPR001731 Porphobilinogen synthase 193 212 1
PR00144 PRINTS IPR001731 Porphobilinogen synthase 245 261 1
PR00144 PRINTS IPR001731 Porphobilinogen synthase 154 173 1
PR00144 PRINTS IPR001731 Porphobilinogen synthase 271 286 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 109.79 Da
Charge -1.50
Isoelectric point 6.17
Molecular weight 36.12 kDa
Number of residues 329
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
53296during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
49105during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
43319during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
53417during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
48175during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
12654.8during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
3561.12during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.45during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
porphyria6
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in bacteria1002
conserved in metazoa3428
conserved in vertebrates3402
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
med7mediator complex subunit Med7 Positive GeneticPMID:22681890
SPCC18.13tRNA (guanine-N7-)-methyltransferase subunit Trm82 (predicted) Positive GeneticPMID:22681890
ssp2serine/threonine protein kinase Ssp2 Positive GeneticPMID:22681890
ypa1protein phosphatase type 2A regulator, PTPA family Ypa1 Positive GeneticPMID:22681890
mkh1MEK kinase (MEKK) Mkh1 Positive GeneticPMID:22681890
xap5xap-5-like protein Positive GeneticPMID:24957674
ngg1SAGA complex subunit Ngg1 Positive GeneticPMID:22681890
SPAC4C5.01haloacid dehalogenase-like hydrolase Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC1805.06c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1805.06c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1805.06c BioGRID Interaction Datasets
Expression Viewer SPAC1805.06c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1805.06c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1805.06c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1805.06c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1805.06c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1805.06c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1805.06c Cell Cycle Data
GEO SPAC1805.06c GEO profiles
PInt SPAC1805.06c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1805.06c Peptides identified in tandem mass spectrometry proteomics experiments
IntEnz4.2.1.24Integrated relational Enzyme database
Rhea4.2.1.24Annotated reactions database
SPD / RIKEN14/14D03Orfeome Localization Data
UniProtKB/SwissProtP78974Delta-aminolevulinic acid dehydratase
ModBaseP78974Database of comparative protein structure models
STRINGP78974Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593917porphobilinogen synthase Hem2 (predicted)
RefSeq mRNANM_001019346972h- porphobilinogen synthase Hem2 (predicted) (hem2), mRNA
European Nucleotide ArchiveU83568ENA EMBL mapping
European Nucleotide ArchiveCAB55847.1ENA Protein Mapping
MetaCycPWY-5188Tetrapyrrole biosynthesis I (from glutamate)
MetaCycPWY-5189Tetrapyrrole biosynthesis II (from glycine)
UniParcUPI000012C45AUniProt Archive
UniPathwayUPA00251Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4

Literature for hem2

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014