uep1 (SPAC1805.12c)


Gene Standard Nameuep1 Characterisation Statuspublished
Systematic IDSPAC1805.12c Feature Typeprotein coding
Synonymsubi2 Name Description
Productribosomal-ubiquitin fusion protein Ubi2 Product Size128aa, 14.60 kDa
Genomic Location Chromosome I, 2794801-2794057 (745nt); CDS:2794724-2794338 (387nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003735structural constituent of ribosomeISOSGD:S000001410GO_REF:0000024255
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0002181cytoplasmic translationNASGO_REF:0000051446
GO:0042254ribosome biogenesisISOSGD:S000001410GO_REF:0000024338
GO:0006412translationISOSGD:S000001410GO_REF:0000024599
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722319
GO:0022625cytosolic large ribosomal subunitISOSGD:S000001410GO_REF:000002482
GO:0005730nucleolusIDAPMID:16823372357
GO:0005634nucleusIDAPMID:168233722737
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyuep1ΔNullPECO:0000005, PECO:0000137PMID:236978063751
Microscopyuep1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopyuep1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
127948012794057
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00240 Pfam IPR000626 Ubiquitin domain 6 74 14
PF01020 Pfam IPR001975 Ribosomal protein L40e 78 128 2
SM00213 SMART IPR000626 Ubiquitin domain 1 72 13
PS50053 Prosite Profiles IPR000626 Ubiquitin domain 1 76 18
PS00299 Prosite Patterns IPR019954 Ubiquitin conserved site 27 52 7
PTHR10666 HMMPANTHER 1 128 6
3.10.20.90 Gene3D 1 96 28
SSF54236 SuperFamily Ubiquitin-related domain 1 95 29
PR00348 PRINTS IPR019956 Ubiquitin 11 31 6
PR00348 PRINTS IPR019956 Ubiquitin 53 74 6
PR00348 PRINTS IPR019956 Ubiquitin 32 52 6

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001447ubiquitin family proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001447
PBO:0001733ribosomal-ubiquitin fusion proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001733

Protein Properties

Ave. residue weight 114.02 Da
Charge 15.00
Isoelectric point 10.48
Molecular weight 14.60 kDa
Number of residues 128
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineIDAS57PMID:182575171670
present during cellular response to thiabendazole
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
42during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
5.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
SPBC31F10.10czf-MYND type zinc finger protein Positive GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
ppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
efc25Ras1 guanyl-nucleotide exchange factor Efc25 Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Positive GeneticPMID:22681890
prp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Positive GeneticPMID:22681890
pst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
sum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
SPAC227.14uridine kinase (predicted) Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rpn1019S proteasome regulatory subunit Rpn10 Co-crystal or NMR structurePMID:20739285
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
rhp23Rad23 homolog Rhp23 Two-hybridPMID:11788722
External References
Database Identifier Description
NBRP SPAC1805.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1805.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1805.12c BioGRID Interaction Datasets
Expression Viewer SPAC1805.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1805.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1805.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1805.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1805.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1805.12c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1805.12c Cell Cycle Data
GEO SPAC1805.12c GEO profiles
PInt SPAC1805.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1805.12c Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN51/51A12Orfeome Localization Data
UniProtKB/SwissProtP0CH07Ubiquitin-60S ribosomal protein L40
ModBaseP0CH07Database of comparative protein structure models
STRINGP0CH07Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593923ubiquitin-60S ribosomal protein L40 fusion protein
RefSeq mRNANM_001019352972h- ubiquitin-60S ribosomal protein L40 fusion protein (uep1), mRNA
ePDB4II2The European PDB
PDB4II2PDB
PDBsum4II2PDBsum
ePDB4II3The European PDB
PDB4II3PDB
PDBsum4II3PDBsum
European Nucleotide ArchiveCAB55853.1ENA Protein Mapping
UniParcUPI0000069E71UniProt Archive

Literature for uep1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014