uep1 (SPAC1805.12c)


Gene Standard Nameuep1 Characterisation Statuspublished
Systematic IDSPAC1805.12c Feature Typeprotein coding
Synonymsubi2 Name Description
Productribosomal-ubiquitin fusion protein Ubi2 Product Size128aa, 14.60 kDa
Genomic Location Chromosome I, 2794801-2794057 (745nt); CDS:2794724-2794338 (387nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
structural constituent of ribosome257
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cytoplasmic translation470
Annotation ExtensionEvidenceWith/FromReference
ribosome biogenesis338
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
cytosolic large ribosomal subunit83
Annotation ExtensionEvidenceWith/FromReference
nucleolus365
Annotation ExtensionEvidenceWith/FromReference
nucleus2691
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
viable vegetative cell populationuep1Δ3844

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologyuep1Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2794801..2794057
mRNA2794801..2794057
5' UTR2794801..2794725PMID:21511999
CDS2794724..2794338
3' UTR2794337..2794057AU014421
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00240 Pfam IPR000626 Ubiquitin domain 6 74 14
PF01020 Pfam IPR001975 Ribosomal protein L40e 78 127 2
SM00213 SMART IPR000626 Ubiquitin domain 1 72 13
PS50053 Prosite Profiles IPR000626 Ubiquitin domain 1 76 18
PS00299 Prosite Patterns IPR019954 Ubiquitin conserved site 27 52 7
PTHR10666 HMMPANTHER 1 127 6
3.10.20.90 Gene3D Ubiquitin 1 96 28
SSF54236 SuperFamily IPR029071 Ubiquitin-related domain 1 95 29
PR00348 PRINTS IPR019956 Ubiquitin 32 52 6
PR00348 PRINTS IPR019956 Ubiquitin 53 74 6
PR00348 PRINTS IPR019956 Ubiquitin 11 31 6

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001447ubiquitin family proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001447
PBO:0001733ribosomal-ubiquitin fusion proteinTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001733

Protein Properties

Ave. residue weight 114.02 Da
Charge 15.00
Codon Adaptation Index 0.69
Isoelectric point 10.48
Molecular weight 14.60 kDa
Number of residues 128
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1677
present during cellular response to thiabendazoleS57
Annotation ExtensionEvidenceResidueReference
present during cellular response to thiabendazole IDA S57 PMID:18257517
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for uep1 (SPAC1805.12c)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
42during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
5.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2505
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC1805.12c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withrhp23Rad23 homolog Rhp23 Two-hybridPMID:11788722
co-locates (via crystalisation or NMR) withrpn1019S proteasome regulatory subunit Rpn10 Co-crystal or NMR structurePMID:20739285
binds activation domain construct withpub3HECT-type ubiquitin-protein ligase E3 Pub3 (predicted) Two-hybridPMID:26771498
binds activation domain construct withrpn1019S proteasome regulatory subunit Rpn10 Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC1805.12c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction witharp42SWI/SNF and RSC complex subunit Arp42 Positive GeneticPMID:22681890
positive genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Positive GeneticPMID:22681890
positive genetic interaction withyfh7uridine kinase Yfh7 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withSPBC31F10.10czf-MYND type zinc finger protein Positive GeneticPMID:22681890
negative genetic interaction withres1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
negative genetic interaction withprp1U4/U6 x U5 tri-snRNP complex subunit Prp1 Negative GeneticPMID:22681890
negative genetic interaction withsum2translation initiation inhibitor (predicted) Negative GeneticPMID:22681890
negative genetic interaction withppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
negative genetic interaction withefc25Ras1 guanyl-nucleotide exchange factor Efc25 Negative GeneticPMID:22681890
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
External References
Database Identifier Description
NBRP SPAC1805.12c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1805.12c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1805.12c BioGRID Interaction Datasets
Expression Viewer SPAC1805.12c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1805.12c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1805.12c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1805.12c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1805.12c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1805.12c Transcriptome Viewer (Bähler Lab)
GEO SPAC1805.12c GEO profiles
PInt SPAC1805.12c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1805.12c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1805.12c Fission yeast phenotypic data & analysis
Cyclebase SPAC1805.12c.1 Cell Cycle Data
SPD / RIKEN51/51A12Orfeome Localization Data
UniProtKB/SwissProtP0CH07Ubiquitin-60S ribosomal protein L40 Ubiquitin 60S ribosomal protein L40
ModBaseP0CH07Database of comparative protein structure models
STRINGP0CH07Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593923ubiquitin-60S ribosomal protein L40 fusion protein
RefSeq mRNANM_001019352972h- ubiquitin-60S ribosomal protein L40 fusion protein (uep1), mRNA
ePDB4II2The European PDB
PDB4II2PDB
PDBsum4II2PDBsum
ePDB4II3The European PDB
PDB4II3PDB
PDBsum4II3PDBsum
European Nucleotide ArchiveCAB55853.1ENA Protein Mapping
UniParcUPI0000069E71UniProt Archive

Literature for uep1

Search: Europe PMC or PubMed

Release Version: PomBase:30_60 - 12 May 2016