pub2 (SPAC1805.15c)


Gene Standard Namepub2 Characterisation Statuspublished
Systematic IDSPAC1805.15c Feature Typeprotein coding
Synonyms Name Description
ProductHECT-type ubiquitin-protein ligase E3 Pub2 Product Size671aa, 77.20 kDa
Genomic Location Chromosome I, 2800406-2797871 (2536nt); CDS:2800231-2798023 (2209nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ubiquitin protein ligase activity60
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
protein ubiquitination115
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell cortex of cell tip42
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4205
Annotation ExtensionEvidenceWith/FromReference
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
membrane1454
Annotation ExtensionEvidenceWith/FromReference
nucleus2740
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001023normal growth on cisplatinCell growth assaypub2ΔNullPECO:0000137, PECO:0000103, PECO:0000005PMID:2124741636
FYPO:0002060viable vegetative cell populationMicroscopypub2ΔNullPMID:204732893759
Microscopypub2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopypub2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
128004062800053
227999952799862
327997662798164
427981222797871

UTRs

Region Coordinates Reference
five_prime_UTR2800406..2800232PMID:21511999
three_prime_UTR2798022..2797871PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00397 Pfam IPR001202 WW domain 244 273 6
PF00632 Pfam IPR000569 HECT 368 669 7
SM00119 SMART IPR000569 HECT 336 671 7
SM00456 SMART IPR001202 WW domain 243 275 9
PS01159 Prosite Patterns IPR001202 WW domain 248 273 4
PS50237 Prosite Profiles IPR000569 HECT 338 671 7
PS50020 Prosite Profiles IPR001202 WW domain 242 275 6
PTHR11254:SF81 HMMPANTHER 7 671 3
PTHR11254 HMMPANTHER 7 671 7
2.20.70.10 Gene3D 239 274 8
2.60.40.150 Gene3D 7 87 19
1c4zA02 Gene3D 441 516 7
SSF51045 SuperFamily IPR001202 WW domain 236 274 9
SSF49562 SuperFamily IPR000008 C2 domain 4 124 16
SSF56204 SuperFamily IPR000569 HECT 306 665 7

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000176ubiquitin-protein ligase E3PMID:11956316Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000176
PBO:0000352WW domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000352
PBO:0001519C2 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001519
PBO:0001736Nedd4/Rsp5 familyPMID:11956316Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001736

Protein Properties

Ave. residue weight 115.05 Da
Charge -9.50
Isoelectric point 5.37
Molecular weight 77.20 kDa
Number of residues 671
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:01148ubiquitinylated lysineIDAC639PMID:1195631622
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0006995experimental evidencePMID:11956316

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
745during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
342.21during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.69during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae YER125W PMID:11956316
Disease Association
DescriptionQualifierReferenceCount
liddle syndrome2
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-WesternPMID:18418059
Reconstituted Complex
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
pst2Clr6 histone deacetylase complex subunit Pst2 Phenotypic EnhancementPMID:19547744
mus7DNA repair protein Mus7/Mms22 Synthetic Growth DefectPMID:18931302
eso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
ryh1GTPase Ryh1 Synthetic Growth DefectPMID:18931302
pub1HECT-type ubiquitin-protein ligase E3 Pub1 Synthetic Growth DefectPMID:11956316
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Synthetic Growth DefectPMID:18931302
External References
Database Identifier Description
NBRP SPAC1805.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1805.15c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1805.15c BioGRID Interaction Datasets
Expression Viewer SPAC1805.15c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1805.15c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1805.15c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1805.15c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1805.15c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1805.15c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1805.15c Cell Cycle Data
GEO SPAC1805.15c GEO profiles
PInt SPAC1805.15c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1805.15c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1805.15c Fission yeast phenotypic data & analysis
SPD / RIKEN50/50A09Orfeome Localization Data
UniProtKB/SwissProtQ9UTG2E3 ubiquitin-protein ligase pub2
ModBaseQ9UTG2Database of comparative protein structure models
STRINGQ9UTG2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593926HECT-type ubiquitin-protein ligase Pub2
RefSeq mRNANM_001019355972h- HECT-type ubiquitin-protein ligase Pub2 (pub2), mRNA
European Nucleotide ArchiveCAB55856.1ENA Protein Mapping
UniParcUPI000006B373UniProt Archive

Literature for pub2

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014