pub2 (SPAC1805.15c)

Gene Standard Namepub2 ChromosomeI
Systematic IDSPAC1805.15c Gene Start2800406
Synonyms Gene End2797871
ProductHECT-type ubiquitin-protein ligase Pub2 Gene Length2536
Feature Typeprotein coding CDS Start2800231
Name Description CDS End2798023
Characterisation Statuspublished CDS Length2209

Ensembl Gene Location
  1. SPAC1805.10
  2. rps2602
  3. uep1
  4. rpl14
  5. SPAC1805.14
  6. pub2
  7. SPAC1805.16c
  8. SPNCRNA.208
  9. SPATRNALEU.02
  10. crm1
Fission Yeast Phenotype Ontology

Ontology Graph

Contact Curators
Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0002060viable vegetative cell populationMicroscopypub2delta (deletion)deletionPMID:20473289
GO Molecular Function

Ontology Graph

Contact Curators
Term IDTerm NameEvidenceWith/FromReference
GO:0004842ubiquitin-protein ligase activity
heterologous_systemIGIpub1PMID:11956316
GO Biological Process

Ontology Graph

Contact Curators
Term IDTerm NameEvidenceWith/FromReference
GO:0016567protein ubiquitinationNASGO_REF:0000051
GO Cellular Component

Ontology Graph

Contact Curators
Term IDTerm NameEvidenceWith/FromReference
GO:0005634nucleusIDAPMID:16823372
IDAPMID:11956316
GO:0005737cytoplasmIDAPMID:11956316
IDAPMID:16823372
GO:0005829cytosolIDAPMID:16823372
GO:0016020membraneIEASL-0162GO_REF:0000039
GO:0051285cell cortex of cell tipIDAPMID:11956316
Transcript
Contact Curators
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
128004062800053
227999952799862
327997662798164
427981222797871

References

Region Start End Reference
three_prime_UTR27980222797871PMID:21511999
five_prime_UTR28004062800232PMID:21511999
three_prime_UTR27980222797871PMID:21511999
Protein Features
Contact Curators

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00632 IPR000569 Pfam HECT 368 669 7
PF00397 IPR001202 Pfam WW domain 244 273 6
SM00456 IPR001202 SMART WW domain 243 275 9
SM00119 IPR000569 SMART HECT 336 671 7
SM00239 IPR000008 SMART C2 calcium-dependent membrane targeting 7 105 15
PS50237 IPR000569 Prosite Profiles HECT 338 671 7
PS50020 IPR001202 Prosite Profiles WW domain 242 275 6
PS01159 IPR001202 Prosite Patterns WW domain 248 273 4
G3DSA:2.60.40.150 Gene3D 7 87 19
G3DSA:2.20.70.10 IPR001202 Gene3D 239 274 6
SSF49562 IPR008973 SuperFamily 4 124 16
SSF56204 IPR000569 SuperFamily 306 665 7
SSF51045 IPR001202 SuperFamily 236 274 9
PTHR11254:SF81 hmmpanther 233 671 3
PTHR11254 hmmpanther 233 671 7

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:01148ubiquitinylated lysineevidenceInferred from Direct AssayPMID:11956316
residueC639

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0000176ubiquitin-protein ligase E3PMID:11956316Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000176
PBO:0000352WW domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000352
PBO:0001519C2 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001519
PBO:0001736Nedd4/Rsp5 familyPMID:11956316Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001736

Protein Properties

Ave. residue weight 115.05 Da
Charge -9.50
Isoelectric point 5.37
Molecular weight 77.20 kDa
Number of residues 671
Sequence
Contact Curators
Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
constitutive PMID:11956316
expressed in response to nitrogen starvation PMID:11956316
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127		population_widePMID:23101633
experimental evidencePECO:0000014,
PECO:0000005		population_wide342.21PMID:23101633
RNA levelexperimental evidencePECO:0000014,
PECO:0000005		population_wide0.69PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.7PMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae YER125W PMID:11956316
Disease Association
DescriptionQualifierReference
liddle syndrome
Species Distribution
DescriptionQualifierReference
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

Contact Curators
Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
pub1HECT-type ubiquitin-protein ligase E3 Pub1 Synthetic Growth DefectPMID:11956316
pst2Clr6 histone deacetylase complex subunit Pst2 Phenotypic EnhancementPMID:19547744
mus7DNA repair protein Mus7/Mms22 Synthetic Growth DefectPMID:18931302
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Synthetic Growth DefectPMID:18931302
eso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
ryh1GTPase Ryh1 Synthetic Growth DefectPMID:18931302
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-WesternPMID:18418059
Reconstituted Complex
Contact Curators
External References
Database Identifier Description
NBRP SPAC1805.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1805.15c Retrieval of eukaryotic orthologs
BioGrid SPAC1805.15c BioGRID Interaction Datasets
Bähler Lab SPAC1805.15c Cell Cycle Expression Profile
Bähler Lab SPAC1805.15c Meiosis/Sporulation Expression Profies
Bähler Lab SPAC1805.15c Pheromone response/mating expression profiles
Bähler Lab SPAC1805.15c Environmental stress expression profiles
Bähler Lab SPAC1805.15c Bähler Lab Transcriptome Viewer
Cyclebase SPAC1805.15c Cell Cycle Data
PInt SPAC1805.15c Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC1805.15c Entrez Gene
WikiGene2542432HECT-type ubiquitin-protein ligase Pub2
Uniprot_genenamePUB2
EntrezGene2542432HECT-type ubiquitin-protein ligase Pub2
UniProtKB/SwissProtQ9UTG2E3 ubiquitin-protein ligase pub2
ModBaseQ9UTG2Database of comparative protein structure models
Pfam Protein DomainsQ9UTG2Pfam Domain Arrangement
RefSeq PeptideNP_593926HECT-type ubiquitin-protein ligase Pub2
RefSeq mRNANM_001019355972h- HECT-type ubiquitin-protein ligase Pub2 (pub2), mRNA
European Nucleotide ArchiveCAB55856ENA Protein Mapping
SPD / RIKEN50/50A09Orfeome Localization Data
UniParcUPI000006B373UniProt Archive

Literature for pub2
Contact Curators

Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013