pub2 (SPAC1805.15c)

Gene Standard Namepub2 Characterisation Statuspublished
Systematic IDSPAC1805.15c Feature Typeprotein coding
Synonyms Name Description
ProductHECT-type ubiquitin-protein ligase E3 Pub2 Product Size671aa, 77.20 kDa
Genomic Location Chromosome I, 2800406-2797871 (2536nt); CDS:2800231-2798023 (2209nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ubiquitin protein ligase activity66
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell cortex of cell tip49
Annotation ExtensionEvidenceWith/FromReference
cell division site318
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
normal growth on cisplatinpub2Δ39
viable vegetative cell populationpub2Δ3862

Cell Phenotype

Term NameGenotypesCount
viable vegetative cell with normal cell morphologypub2Δ3103

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased cell population growth at high temperaturepub1Δ, pub2Δ, clp1+

Cell Phenotype

Term NameGenotypes
inviable branched, elongated vegetative cellclp1Δ, pub2+
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2800406..2800053, 2799995..2799862, 2799766..2798164, 2798122..2797871
Intron2800052..2799996, 2799861..2799767, 2798163..2798123
5' UTR2800406..2800232PMID:21511999
CDS2800231..2800053, 2799995..2799862, 2799766..2798164, 2798122..2798023
3' UTR2798022..2797871PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00632 Pfam IPR000569 HECT domain 368 670 7
PF00397 Pfam IPR001202 WW domain 244 273 9
SM00119 SMART IPR000569 HECT domain 336 671 7
SM00456 SMART IPR001202 WW domain 243 275 9
PS01159 Prosite Patterns IPR001202 WW domain 248 273 4
PS50020 Prosite Profiles IPR001202 WW domain 242 275 6
PS50237 Prosite Profiles IPR000569 HECT domain 338 671 7
PTHR11254 HMMPANTHER 54 671 7 Gene3D IPR000008 C2 domain 7 87 19 Gene3D Nudix hydrolase, N-terminal 239 274 8
1c4zA02 Gene3D 441 516 7
SSF51045 SuperFamily IPR001202 WW domain 236 274 9
SSF56204 SuperFamily IPR000569 HECT domain 306 665 7
SSF49562 SuperFamily IPR000008 C2 domain 4 124 16

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000176ubiquitin-protein ligase E3PMID:11956316Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000176
PBO:0000352WW domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000352
PBO:0001519C2 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001519
PBO:0001736Nedd4/Rsp5 familyPMID:11956316Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001736

Protein Properties

Ave. residue weight 115.05 Da
Charge -9.50
Codon Adaptation Index 0.41
Isoelectric point 5.37
Molecular weight 77.20 kDa
Number of residues 671

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS280, S284, S289 2293
Annotation ExtensionEvidenceResidueReference
IDA S280 PMID:25720772
IDA S284 PMID:25720772
IDA S289 PMID:25720772
O-phospho-L-threonineT287 1087
Annotation ExtensionEvidenceResidueReference
IDA T287 PMID:25720772
ubiquitinylated lysineC639 514
Annotation ExtensionEvidenceResidueReference
IDA C639 PMID:11956316
Gene Expression

Qualitative Gene Expression

RNA levelincreased during GO:0006995experimental evidencePMID:11956316

Quantitative Gene Expression

View graphical display of gene expression data for pub2 (SPAC1805.15c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
342.21during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
745during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.69during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
0.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
functionally complemented by S. cerevisiae YER125W PMID:11956316
Disease Association
liddle syndrome2
Taxonomic Conservation
conserved in fungi4608
conserved in eukaryotes4516
conserved in metazoa3498
conserved in vertebrates3473
conserved in eukaryotes only2525

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC1805.15c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturesclp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-WesternPMID:18418059
forms complex withclp1Cdc14-related protein phosphatase Clp1/Flp1 Reconstituted ComplexPMID:18418059
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC1805.15c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
enhances phenotype ofpub1HECT-type ubiquitin-protein ligase E3 Pub1 Phenotypic EnhancementPMID:26365378
enhances phenotype ofpst2Clr6 histone deacetylase complex subunit Pst2 Phenotypic EnhancementPMID:19547744
synthetic growth defect withmus7DNA repair protein Mus7/Mms22 Synthetic Growth DefectPMID:18931302
synthetic growth defect withryh1GTPase Ryh1 Synthetic Growth DefectPMID:18931302
synthetic growth defect withpub1HECT-type ubiquitin-protein ligase E3 Pub1 Synthetic Growth DefectPMID:11956316
synthetic growth defect withpef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Synthetic Growth DefectPMID:18931302
phenotype enhanced byubc13ubiquitin conjugating enzyme E2 Ubc13 Phenotypic EnhancementPMID:26365378
negative genetic interaction witheso1mitotic cohesin N-acetyltransferase/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
External References
Database Identifier Description
NBRP SPAC1805.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1805.15c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1805.15c BioGRID Interaction Datasets
Expression Viewer SPAC1805.15c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1805.15c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1805.15c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1805.15c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1805.15c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1805.15c Transcriptome Viewer (Bähler Lab)
GEO SPAC1805.15c GEO profiles
PInt SPAC1805.15c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1805.15c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1805.15c Fission yeast phenotypic data & analysis
Cyclebase SPAC1805.15c.1 Cell Cycle Data
SPD / RIKEN50/50A09Orfeome Localization Data
UniProtKB/SwissProtQ9UTG2E3 ubiquitin-protein ligase pub2
ModBaseQ9UTG2Database of comparative protein structure models
STRINGQ9UTG2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593926HECT-type ubiquitin-protein ligase Pub2
RefSeq mRNANM_001019355972h- HECT-type ubiquitin-protein ligase Pub2 (pub2), mRNA
European Nucleotide ArchiveCAB55856.1ENA Protein Mapping
UniParcUPI000006B373UniProt Archive

Literature for pub2

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017