pub2 (SPAC1805.15c)


Gene Standard Namepub2 Characterisation Statuspublished
Systematic IDSPAC1805.15c Feature Typeprotein coding
Synonyms Name Description
ProductHECT-type ubiquitin-protein ligase Pub2 Product Size671aa, 77.20 kDa
Genomic Location Chromosome I, 2800406-2797871 (2536nt); CDS:2800231-2798023 (2209nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004842ubiquitin-protein ligase activity83
heterologous_systemIGIpub1PMID:11956316
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0016567protein ubiquitinationIGIpub1PMID:11956316115
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0051285cell cortex of cell tipIDAPMID:1195631638
GO:0005737cytoplasmIDAPMID:168233724198
IDAPMID:11956316
GO:0005829cytosolIDAPMID:168233722317
GO:0016020membraneIEAUniProtKB-KW:KW-0472GO_REF:00000371439
GO:0005634nucleusIDAPMID:119563162730
IDAPMID:16823372
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001023normal cell population growth on cisplatinCell growth assaypub2ΔNullPECO:0000137, PECO:0000103, PECO:0000005PMID:2124741636
FYPO:0002060viable vegetative cell populationMicroscopypub2ΔNullPECO:0000005, PECO:0000137PMID:236978063755
Microscopypub2ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopypub2ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
128004062800053
227999952799862
327997662798164
427981222797871

UTRs

Region Start End Reference
three_prime_UTR27980222797871PMID:21511999
five_prime_UTR28004062800232PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00632 Pfam IPR000569 HECT 368 669 7
PF00397 Pfam IPR001202 WW domain 244 273 6
SM00456 SMART IPR001202 WW domain 243 275 9
SM00119 SMART IPR000569 HECT 336 671 7
SM00239 SMART IPR000008 C2 domain 7 105 15
PS50237 Prosite Profiles IPR000569 HECT 338 671 7
PS50020 Prosite Profiles IPR001202 WW domain 242 275 6
PS01159 Prosite Patterns IPR001202 WW domain 248 273 4
PTHR11254 HMMPANTHER 7 671 7
PTHR11254:SF81 HMMPANTHER 7 671 3
G3DSA:2.60.40.150 Gene3D 7 87 19
G3DSA:2.20.70.10 Gene3D 239 274 6
G3DSA:3.30.2160.10 Gene3D 441 516 7
SSF49562 SuperFamily IPR000008 4 124 16
SSF56204 SuperFamily IPR000569 306 665 7
SSF51045 SuperFamily IPR001202 236 274 9

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000176ubiquitin-protein ligase E3PMID:11956316Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000176
PBO:0000352WW domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000352
PBO:0001519C2 domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001519
PBO:0001736Nedd4/Rsp5 familyPMID:11956316Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001736

Protein Properties

Ave. residue weight 115.05 Da
Charge -9.50
Isoelectric point 5.37
Molecular weight 77.20 kDa
Number of residues 671
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:01148ubiquitinylated lysineIDAC639PMID:1195631616
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
342.21during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
0.69during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReferenceCount
functionally complemented by S. cerevisiae YER125W PMID:119563161
Disease Association
DescriptionQualifierReferenceCount
liddle syndrome2
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
pst2Clr6 histone deacetylase complex subunit Pst2 Phenotypic EnhancementPMID:19547744
mus7DNA repair protein Mus7/Mms22 Synthetic Growth DefectPMID:18931302
eso1sister chromatid cohesion protein/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
ryh1GTPase Ryh1 Synthetic Growth DefectPMID:18931302
pub1HECT-type ubiquitin-protein ligase E3 Pub1 Synthetic Growth DefectPMID:11956316
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Synthetic Growth DefectPMID:18931302
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
clp1Cdc14-related protein phosphatase Clp1/Flp1 Affinity Capture-WesternPMID:18418059
Reconstituted Complex
External References
Database Identifier Description
NBRP SPAC1805.15c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1805.15c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1805.15c BioGRID Interaction Datasets
Expression Viewer SPAC1805.15c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1805.15c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1805.15c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1805.15c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1805.15c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1805.15c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1805.15c Cell Cycle Data
GEO SPAC1805.15c GEO profiles
PInt SPAC1805.15c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542432HECT-type ubiquitin-protein ligase Pub2
EntrezGene2542432HECT-type ubiquitin-protein ligase Pub2
SPD / RIKEN50/50A09Orfeome Localization Data
UniProtKB/SwissProtQ9UTG2E3 ubiquitin-protein ligase pub2
ModBaseQ9UTG2Database of comparative protein structure models
StringQ9UTG2Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593926HECT-type ubiquitin-protein ligase Pub2
RefSeq mRNANM_001019355972h- HECT-type ubiquitin-protein ligase Pub2 (pub2), mRNA
European Nucleotide ArchiveCAB55856ENA Protein Mapping
UniParcUPI000006B373UniProt Archive

Literature for pub2

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014