hhf1 (SPAC1834.03c)


Gene Standard Namehhf1 Characterisation Statuspublished
Systematic IDSPAC1834.03c Feature Typeprotein coding
Synonymsh4.1 Name Description
Producthistone H4 h4.1 Product Size103aa, 11.42 kDa
Genomic Location Chromosome I, 4699544-4698773 (772nt); CDS:4699396-4699085 (312nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
DNA binding379
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
nucleosome assembly28
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nuclear nucleosome10
Annotation ExtensionEvidenceWith/FromReference
nucleus2695
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationhhf1ΔNull3795

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologyhhf1ΔNull3093
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in ams2 cell cycle regulated GATA-type transcription factor Ams2 PMID:17452352
FYPO affected by mutation in ams2 cell cycle regulated GATA-type transcription factor Ams2 PMID:20230746
FYPO affected by mutation in hip1 hira protein, histone chaperone Hip1 PMID:17452352
FYPO affected by mutation in hsk1 Dbf4(Dfp1)-dependent protein kinase Hsk1 PMID:20230746
FYPO affected by mutation in pdp1 PWWP domain protein Pdp1 PMID:19250904
FYPO affected by mutation in set9 histone lysine H3-K20 methyltransferase Set9 PMID:19250904
FYPO affected by mutation in slm9 hira protein Slm9 PMID:17452352
FYPO affected by mutation in spt16 FACT complex subunit Spt16 PMID:18579787
GO regulated by ams2 cell cycle regulated GATA-type transcription factor Ams2 PMID:17452352
GO regulated by ams2 cell cycle regulated GATA-type transcription factor Ams2 PMID:20230746
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4699544..4698773
mRNA4699544..4698773
5' UTR4699544..4699397SPC00519
CDS4699396..4699085
3' UTR4699084..4698773SPC00366
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00125 Pfam IPR007125 Histone core 28 94 11
SM00417 SMART IPR001951 Histone H4 16 90 3
PS00047 Prosite Patterns IPR019809 Histone H4, conserved site 15 19 3
PTHR10484 HMMPANTHER 1 103 5
1.10.20.10 Gene3D IPR009072 Histone-fold 2 103 27
SSF47113 SuperFamily IPR009072 Histone-fold 3 102 26
PR00623 PRINTS IPR001951 Histone H4 62 76 3
PR00623 PRINTS IPR001951 Histone H4 20 39 3
PR00623 PRINTS IPR001951 Histone H4 89 100 3
PR00623 PRINTS IPR001951 Histone H4 77 89 3
PR00623 PRINTS IPR001951 Histone H4 5 16 3
PR00623 PRINTS IPR001951 Histone H4 40 60 3

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000297histone foldTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000297

Protein Properties

Ave. residue weight 110.91 Da
Charge 19.00
Isoelectric point 11.91
Molecular weight 11.42 kDa
Number of residues 103
Modifications

Protein Modifications

Term NameResidueCount
acetylated residue 14
Annotation ExtensionEvidenceResidueReference
ISS PMID:0000001
methylated residueK20 17
Annotation ExtensionEvidenceResidueReference
ISS K20 PMID:0000001
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
181226during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
202963during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
170140during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
190516during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
201432during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
28during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
3.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byair1zinc knuckle TRAMP complex subunit Air1 Affinity Capture-MSPMID:20403971
(peptide) binds to proteinbdf2BET family double bromodomain protein Bdf2 Protein-peptidePMID:24013502
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured bycia1histone chaperone Cia1 Affinity Capture-MSPMID:21211723
forms complex withcrb2DNA repair protein Rad9 homolog Crb2 Reconstituted ComplexPMID:18826944
affinity captured bycyk3cytokinesis protein Cyk3 (predicted) Affinity Capture-MSPMID:20603077
modified byhat1histone acetyltransferase Hat1 Biochemical ActivityPMID:22771823
affinity captured bymtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
affinity captured bynts1Clr6 histone deacetylase complex subunit Nts1 Affinity Capture-MSPMID:25002536
affinity captured bypdp1PWWP domain protein Pdp1 Affinity Capture-WesternPMID:19250904
(peptide) binds to proteinpdp1PWWP domain protein Pdp1 Protein-peptidePMID:22150589
affinity captured byred1RNA elimination defective protein Red1 Affinity Capture-MSPMID:24713849
affinity captured byred5human ZC3H3 homolog Affinity Capture-MSPMID:24713849
affinity captured byrik1silencing protein Rik1 Affinity Capture-MSPMID:17114925
affinity captured byrmn1RNA-binding protein Affinity Capture-MSPMID:24713849
forms complex withrna1Ran GAP Rna1 Reconstituted ComplexPMID:16540522
affinity captured byrng2IQGAP Affinity Capture-MSPMID:20603077
modified byset9histone lysine H3-K20 methyltransferase Set9 Biochemical ActivityPMID:15550243
modified bysir2Sirtuin family histone deacetylase Sir2 Biochemical ActivityPMID:24013502
forms complex withspt16FACT complex subunit Spt16 Reconstituted ComplexPMID:18579787
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withacp2F-actin capping protein beta subunit Acp2 Negative GeneticPMID:22681890
overexpression rescuesams2cell cycle regulated GATA-type transcription factor Ams2 Dosage RescuePMID:12535531
negative genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
negative genetic interaction withapc10anaphase-promoting complex substrate recognition subunit Apc10 Negative GeneticPMID:22681890
negative genetic interaction withask1DASH complex subunit Ask1 Negative GeneticPMID:18818364
negative genetic interaction withask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
rescuesbdf2BET family double bromodomain protein Bdf2 Phenotypic SuppressionPMID:24013502
negative genetic interaction withcay1cactin, spliceosome complex subunit (predicted) Negative GeneticPMID:18818364
positive genetic interaction withcbp1CENP-B homolog Positive GeneticPMID:18818364
overexpression rescuescnp1centromere-specific histone H3 CENP-A Dosage RescuePMID:25298518
overexpression rescuescnp1centromere-specific histone H3 CENP-A Dosage RescuePMID:12535531
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:18818364
negative genetic interaction withdad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
negative genetic interaction withelp5elongator complex subunit Elp5 (predicted) Negative GeneticPMID:18818364
rescuesepe1Jmjc domain chromatin associated protein Epe1 Phenotypic SuppressionPMID:24013502
positive genetic interaction withfml2ATP-dependent 3' to 5' DNA helicase (predicted) Positive GeneticPMID:22681890
negative genetic interaction withgar2nucleolar protein required for rRNA processing Negative GeneticPMID:22681890
negative genetic interaction withhhf2histone H4 h4.2 Negative GeneticPMID:18818364
negative genetic interaction withhhf2histone H4 h4.2 Negative GeneticPMID:22681890
negative genetic interaction withhip1hira protein, histone chaperone Hip1 Negative GeneticPMID:18818364
negative genetic interaction withhip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withmcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:18818364
negative genetic interaction withmcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:22681890
negative genetic interaction withnup132nucleoporin Nup132 Negative GeneticPMID:22681890
negative genetic interaction withpab2poly(A) binding protein Pab2 Negative GeneticPMID:22681890
negative genetic interaction withpfl9cell surface glycoprotein (predicted), DIPSY family Negative GeneticPMID:22681890
negative genetic interaction withpof3F-box protein Pof3 Negative GeneticPMID:22681890
positive genetic interaction withpom1DYRK family protein kinase Pom1 Positive GeneticPMID:18818364
negative genetic interaction withrok1ATP-dependent RNA helicase Rok1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withryh1GTPase Ryh1 Positive GeneticPMID:22681890
negative genetic interaction withsds23PP2A-type phosphatase inhibitor Sds23/Moc1 Negative GeneticPMID:22681890
positive genetic interaction withSPAC186.05chuman TMEM165 homolog, congenital disorders of glycosylation Positive GeneticPMID:22681890
positive genetic interaction withSPAC25B8.08conserved fungal protein Positive GeneticPMID:22681890
negative genetic interaction withSPAC977.13cNegative GeneticPMID:22681890
positive genetic interaction withSPBC1348.07S. pombe specific DUF999 protein family 6 Positive GeneticPMID:22681890
negative genetic interaction withSPBC36B7.08cnucleosome assembly protein (predicted) Negative GeneticPMID:18818364
negative genetic interaction withSPBC36B7.08cnucleosome assembly protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withspc34DASH complex subunit Spc34 Negative GeneticPMID:18818364
negative genetic interaction withspc34DASH complex subunit Spc34 Negative GeneticPMID:22681890
negative genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
negative genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Negative GeneticPMID:18818364
negative genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Negative GeneticPMID:22681890
positive genetic interaction withvps35retromer complex subunit Vps35 Positive GeneticPMID:22681890
negative genetic interaction withyaf9YEATS family histone acetyltransferase subunit Yaf9 Negative GeneticPMID:18818364
External References
Database Identifier Description
NBRP SPAC1834.03c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1834.03c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1834.03c BioGRID Interaction Datasets
Expression Viewer SPAC1834.03c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1834.03c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1834.03c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1834.03c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1834.03c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1834.03c Transcriptome Viewer (Bähler Lab)
GEO SPAC1834.03c GEO profiles
PInt SPAC1834.03c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1834.03c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1834.03c Fission yeast phenotypic data & analysis
Cyclebase SPAC1834.03c.1 Cell Cycle Data
SPD / RIKEN01/01F02Orfeome Localization Data
UniProtKB/SwissProtP09322Histone H4
ModBaseP09322Database of comparative protein structure models
STRINGP09322Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594682histone H4 h4.1
RefSeq mRNANM_001020111972h- histone H4 h4.1 (hhf1), mRNA
European Nucleotide ArchiveBAA21442.1ENA Protein Mapping
European Nucleotide ArchiveCAA17818.1ENA Protein Mapping
European Nucleotide ArchiveCAA28850.1ENA Protein Mapping
European Nucleotide ArchiveCAA28853.1ENA Protein Mapping
European Nucleotide ArchiveCAA28855.1ENA Protein Mapping
European Nucleotide ArchiveCAB50975.1ENA Protein Mapping
European Nucleotide ArchiveCAB75771.1ENA Protein Mapping
UniParcUPI0000162095UniProt Archive

Literature for hhf1

Search: Europe PMC or PubMed

Release Version: PomBase:26_51 - 07 Apr 2015