hht1 (SPAC1834.04)

Gene Standard Namehht1 Characterisation Statuspublished
Systematic IDSPAC1834.04 Feature Typeprotein coding
Synonyms Name Description
Producthistone H3 h3.1 Product Size136aa, 15.36 kDa
Genomic Location Chromosome I, 4699691-4700560 (870nt); CDS:4699829-4700239 (411nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0003677DNA bindingIEAUniProtKB-KW:KW-0238GO_REF:0000037385
GO:0005515protein binding865
active form PR:000027578IPIswi6PMID:11242054
active form PR:000027576IPIbir1PMID:20929775
GO:0046982protein heterodimerization activityIEAIPR009072GO_REF:000000227
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006974cellular response to DNA damage stimulusIMPPMID:17369611213
GO:0006333chromatin assembly or disassemblyNASGO_REF:000000143
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0031934mating-type region heterochromatin25
localised form PR:000027576IDAPMID:20929775
GO:0000788nuclear nucleosomeNASGO_REF:000000110
GO:0031618nuclear pericentric heterochromatin38
localised form PR:000027576IDAPMID:20929775
GO:0005724nuclear telomeric heterochromatin24
localised form PR:000027576IDAPMID:20929775
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000266sensitive to DNA damaging agentsCell growth assayK56R (K56R)PMID:173696116
FYPO:0000267sensitive to ionizing radiationCell growth assayK56R (K56R)PMID:1736961144
FYPO:0002060viable vegetative cell populationMicroscopyhht1ΔNullPECO:0000005, PECO:0000137PMID:236978063751

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001861increased minichromosome loss upon segregation during vegetative growthPlasmid maintenance assay evidencehht1+ (wild type)OverexpressionPMID:247101269
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopyhht1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
FYPO affected by mutation in mis18 kinetochore protein Mis18 PMID:19217403
FYPO affected by mutation in scm3 CENP-A chaperone Scm3 PMID:19217403
FYPO affected by mutation in sim3 NASP family CENP-A chaperone PMID:18158900
GO regulated by ams2 cell cycle regulated GATA-type transcription factor Ams2 PMID:17452352
GO regulated by ams2 cell cycle regulated GATA-type transcription factor Ams2 PMID:20230746
GO substrate of ark1 aurora-B kinase Ark1 PMID:11792803
GO substrate of hrk1 haspin related kinase Hrk1 PMID:20929775
GO substrate of rtt109 RTT109 family histone lysine acetyltransferase PMID:17369611
Ensembl transcript structure with UTRs, exons and introns


Exon Start End
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00125 Pfam IPR007125 Histone core 58 132 11
SM00428 SMART IPR000164 Histone H3 34 136 4
PS00322 Prosite Patterns IPR000164 Histone H3 15 21 3
PS00959 Prosite Patterns IPR000164 Histone H3 67 75 3
PTHR11426 HMMPANTHER IPR000164 Histone H3 1 135 4 Gene3D IPR009072 Histone-fold 2 134 27
SSF47113 SuperFamily IPR009072 Histone-fold 2 133 26
PR00622 PRINTS IPR000164 Histone H3 98 114 4
PR00622 PRINTS IPR000164 Histone H3 114 135 4
PR00622 PRINTS IPR000164 Histone H3 3 17 4
PR00622 PRINTS IPR000164 Histone H3 80 98 4
PR00622 PRINTS IPR000164 Histone H3 17 31 4
PR00622 PRINTS IPR000164 Histone H3 58 75 4
PR00622 PRINTS IPR000164 Histone H3 34 55 4

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000297histone foldTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000297

Protein Properties

Ave. residue weight 112.92 Da
Charge 21.00
Isoelectric point 11.83
Molecular weight 15.36 kDa
Number of residues 136

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS11PMID:247631071670
MOD:00427methylated residueNASK14PMID:145613999
present during chromatin silencing at centromere
present during chromatin silencing at centromereNASK9PMID:14561399
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5289during GO:0000080PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
5036during GO:0000084PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
6570during GO:0000085PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
5075during GO:0000087PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
NDduring GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
5429during GO:0072690PECO:0000126,
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
28during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
2.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
conserved in fungi4599
conserved in eukaryotes4514
conserved in metazoa3428
conserved in vertebrates3402
conserved in eukaryotes only2498

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
med20mediator complex subunit Med20 Positive GeneticPMID:18818364
SPCC18.13tRNA (guanine-N7-)-methyltransferase subunit Trm82 (predicted) Negative GeneticPMID:22681890
acp2F-actin capping protein beta subunit Acp2 Negative GeneticPMID:22681890
par1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:18818364
mcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:18818364
ilv1acetolactate synthase catalytic subunit Negative GeneticPMID:22681890
alg10dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase Alg10 (predicted) Negative GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Dosage LethalityPMID:16540522
rpt319S proteasome regulatory subunit Rpt3 (predicted) Dosage RescuePMID:24710126
rdp1RNA-directed RNA polymerase Rdp1 Negative GeneticPMID:18818364
pht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
cay1cactin, spliceosome complex subunit (predicted) Negative GeneticPMID:18818364
erp2COPII-coated vesicle component Erp2/3/4 (predicted) Negative GeneticPMID:22681890
hht2histone H3 h3.2 Negative GeneticPMID:18818364
rhn1RNA polymerase II transcription termination factor homolog Negative GeneticPMID:18818364
swd1Set1C complex subunit Swd1 Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
snf21ATP-dependent DNA helicase Snf21 Protein-peptidePMID:22184112
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
bir1survivin, Bir1 Affinity Capture-WesternPMID:20929775
rna1Ran GAP Rna1 Reconstituted ComplexPMID:16540522
Biochemical Activity
rik1silencing protein Rik1 Affinity Capture-WesternPMID:20705239
swi6HP1 family chromodomain protein Swi6 Protein-peptidePMID:22727667
Reconstituted ComplexPMID:21211724
sim3NASP family CENP-A chaperone Affinity Capture-WesternPMID:18158900
Reconstituted Complex
rsc4RSC complex subunit Rsc4 Affinity Capture-WesternPMID:22184112
bdf2BET family double bromodomain protein Bdf2 Protein-peptidePMID:24013502
Affinity Capture-MS
spt16FACT complex subunit Spt16 Reconstituted ComplexPMID:18579787
clr4histone H3 lysine methyltransferase Clr4 Protein-peptidePMID:22727667
Reconstituted ComplexPMID:18345014
Biochemical ActivityPMID:23849629
Reconstituted ComplexPMID:16540522
Biochemical Activity
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
paa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
chp1chromodomain protein Chp1 Protein-peptidePMID:22727667
cia1histone chaperone Cia1 Affinity Capture-WesternPMID:22291963
Affinity Capture-MSPMID:21211723
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
hrk1haspin related kinase Hrk1 Biochemical ActivityPMID:20929775
Affinity Capture-Western
External References
Database Identifier Description
NBRP SPAC1834.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1834.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1834.04 BioGRID Interaction Datasets
Expression Viewer SPAC1834.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1834.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1834.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1834.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1834.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1834.04 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1834.04 Cell Cycle Data
GEO SPAC1834.04 GEO profiles
PInt SPAC1834.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1834.04 Peptides identified in tandem mass spectrometry proteomics experiments
SPD / RIKEN03/03B12Orfeome Localization Data
UniProtKB/SwissProtP09988Histone H3.1/H3.2
ModBaseP09988Database of comparative protein structure models
STRINGP09988Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594683histone H3 h3.1
RefSeq mRNANM_001020112972h- histone H3 h3.1 (hht1), mRNA
ePDB2DZEThe European PDB
ePDB3G7LThe European PDB
European Nucleotide ArchiveBAA21441.1ENA Protein Mapping
European Nucleotide ArchiveCAA17819.1ENA Protein Mapping
European Nucleotide ArchiveCAA28851.1ENA Protein Mapping
European Nucleotide ArchiveCAA28852.1ENA Protein Mapping
European Nucleotide ArchiveCAB75772.1ENA Protein Mapping
UniParcUPI0000162096UniProt Archive

Literature for hht1

Search: Europe PMC or PubMed

Release Version: PomBase:23_46 - 30 Aug 2014