hht1 (SPAC1834.04)


Gene Standard Namehht1 Characterisation Statuspublished
Systematic IDSPAC1834.04 Feature Typeprotein coding
Synonyms Name Description
Producthistone H3 h3.1 Product Size136aa, 15.36 kDa
Genomic Location Chromosome I, 4699691-4700560 (870nt); CDS:4699829-4700239 (411nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
DNA binding379
Annotation ExtensionEvidenceWith/FromReference
protein binding859
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular response to DNA damage stimulus210
Annotation ExtensionEvidenceWith/FromReference
chromatin assembly or disassembly70
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mating-type region heterochromatin26
Annotation ExtensionEvidenceWith/FromReference
nuclear nucleosome10
Annotation ExtensionEvidenceWith/FromReference
nuclear pericentric heterochromatin39
Annotation ExtensionEvidenceWith/FromReference
nuclear subtelomeric heterochromatin18
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
increased viability upon nitrogen starvationhht1ΔNull54
sensitive to DNA damaging agentsK56R (K56R)7
sensitive to ionizing radiationK56R (K56R)60
viable vegetative cell populationhht1ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
increased minichromosome loss upon segregation during vegetative growthhht1+ (wild type)Overexpression15
viable vegetative cell with normal cell morphologyhht1ΔNull3094
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in ams2 cell cycle regulated GATA-type transcription factor Ams2
FYPO affected by mutation in hip1 hira protein, histone chaperone Hip1
FYPO affected by mutation in hsk1 Dbf4(Dfp1)-dependent protein kinase Hsk1
FYPO affected by mutation in mis18 kinetochore protein Mis18
FYPO affected by mutation in scm3 CENP-A chaperone Scm3
FYPO affected by mutation in sim3 NASP family CENP-A chaperone
FYPO affected by mutation in slm9 hira protein Slm9
GO regulated by ams2 cell cycle regulated GATA-type transcription factor Ams2
GO substrate of ark1 aurora-B kinase Ark1
GO substrate of hrk1 haspin related kinase Hrk1
GO substrate of rtt109 RTT109 family histone lysine acetyltransferase
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4699691..4700560
mRNA4699691..4700560
5' UTR4699691..4699828PMID:21511999
CDS4699829..4700239
3' UTR4700240..4700560SPC03596
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00125 Pfam IPR007125 Histone core 58 132 11
SM00428 SMART IPR000164 Histone H3/CENP-A 34 136 4
PS00322 Prosite Patterns IPR000164 Histone H3/CENP-A 15 21 3
PS00959 Prosite Patterns IPR000164 Histone H3/CENP-A 67 75 3
PTHR11426 HMMPANTHER IPR000164 Histone H3/CENP-A 1 135 4
1.10.20.10 Gene3D IPR009072 Histone-fold 2 134 27
SSF47113 SuperFamily IPR009072 Histone-fold 2 133 26
PR00622 PRINTS IPR000164 Histone H3/CENP-A 80 98 4
PR00622 PRINTS IPR000164 Histone H3/CENP-A 3 17 4
PR00622 PRINTS IPR000164 Histone H3/CENP-A 34 55 4
PR00622 PRINTS IPR000164 Histone H3/CENP-A 98 114 4
PR00622 PRINTS IPR000164 Histone H3/CENP-A 17 31 4
PR00622 PRINTS IPR000164 Histone H3/CENP-A 58 75 4
PR00622 PRINTS IPR000164 Histone H3/CENP-A 114 135 4

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000297histone foldTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000297

Protein Properties

Ave. residue weight 112.92 Da
Charge 21.00
Isoelectric point 11.83
Molecular weight 15.36 kDa
Number of residues 136
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS11 1672
Annotation ExtensionEvidenceResidueReference
experimental evidence S11 PMID:24763107
N6-acetyl-L-lysineK56 4
Annotation ExtensionEvidenceResidueReference
IDA K56 PMID:17369611
acetylated residue 14
Annotation ExtensionEvidenceResidueReference
ISS PMID:0000001
methylated residue 17
present during chromatin silencing at centromereK9
K4
present during chromatin silencing at centromereK14
Annotation ExtensionEvidenceResidueReference
ISS K4 PMID:0000001
present during chromatin silencing at centromere NAS K9 PMID:14561399
present during chromatin silencing at centromere NAS K14 PMID:14561399
methylated lysine 7
Annotation ExtensionEvidenceResidueReference
IDA PMID:18292091
N-acetylated L-lysine 4
added by mst1K4
added during mitotic S phaseK4
K9
added during mitotic S phaseK56
Annotation ExtensionEvidenceResidueReference
IDA PMID:18292091
added by mst1 IDA K4 PMID:20299449
added during mitotic S phase IDA K4 PMID:20299449
IDA K9 PMID:20299449
added during mitotic S phase IDA K56 PMID:20299449
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5289during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5036during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6570during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5075during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5429during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
28during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
(peptide) binds to proteinbdf2BET family double bromodomain protein Bdf2 Protein-peptidePMID:24013502
affinity capturesbir1survivin, Bir1 Affinity Capture-WesternPMID:20929775
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
binds to peptidechp1chromodomain protein Chp1 Protein-peptidePMID:22727667
(peptide) binds to proteinchp1chromodomain protein Chp1 Protein-peptidePMID:20299449
(peptide) binds to proteinchp2HP1 family chromodomain protein 2 Protein-peptidePMID:20299449
affinity captured bycia1histone chaperone Cia1 Affinity Capture-WesternPMID:22291963
affinity captured bycia1histone chaperone Cia1 Affinity Capture-MSPMID:21211723
binds to peptideclr4histone H3 lysine methyltransferase Clr4 Protein-peptidePMID:22727667
forms complex withclr4histone H3 lysine methyltransferase Clr4 Reconstituted ComplexPMID:18345014
modified byclr4histone H3 lysine methyltransferase Clr4 Biochemical ActivityPMID:23849629
modified byclr4histone H3 lysine methyltransferase Clr4 Biochemical ActivityPMID:16540522
forms complex withclr4histone H3 lysine methyltransferase Clr4 Reconstituted ComplexPMID:16540522
affinity captured bycwf14G10 protein Affinity Capture-MSPMID:24874881
affinity captured byhrk1haspin related kinase Hrk1 Affinity Capture-WesternPMID:20929775
modified byhrk1haspin related kinase Hrk1 Biochemical ActivityPMID:20929775
affinity captured bymtl1TRAMP complex ATP-dependent RNA helicase (predicted) Affinity Capture-MSPMID:24713849
affinity captured bypaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
affinity capturesrik1silencing protein Rik1 Affinity Capture-WesternPMID:20705239
affinity captured byrmn1RNA-binding protein Affinity Capture-MSPMID:24713849
modifiesrna1Ran GAP Rna1 Biochemical ActivityPMID:16540522
forms complex withrna1Ran GAP Rna1 Reconstituted ComplexPMID:16540522
affinity capturesrsc4RSC complex subunit Rsc4 Affinity Capture-WesternPMID:22184112
binds to peptidersc4RSC complex subunit Rsc4 Protein-peptidePMID:22184112
affinity captured byset2histone lysine methyltransferase Set2 Affinity Capture-WesternPMID:24583182
affinity capturessim3NASP family CENP-A chaperone Affinity Capture-WesternPMID:18158900
affinity captured bysim3NASP family CENP-A chaperone Affinity Capture-WesternPMID:18158900
binds DNA-binding domain construct withsim3NASP family CENP-A chaperone Two-hybridPMID:18158900
forms complex withsim3NASP family CENP-A chaperone Reconstituted ComplexPMID:18158900
binds to peptidesnf21ATP-dependent DNA helicase Snf21 Protein-peptidePMID:22184112
forms complex withspt16FACT complex subunit Spt16 Reconstituted ComplexPMID:18579787
binds to peptideswi6HP1 family chromodomain protein Swi6 Protein-peptidePMID:22727667
forms complex withswi6HP1 family chromodomain protein Swi6 Reconstituted ComplexPMID:21211724
(peptide) binds to proteinswi6HP1 family chromodomain protein Swi6 Protein-peptidePMID:20299449
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withacp2F-actin capping protein beta subunit Acp2 Negative GeneticPMID:22681890
negative genetic interaction withalg10dolichyl-phosphate-glucose-glycolipid alpha-glucosyltransferase Alg10 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withcay1cactin, spliceosome complex subunit Negative GeneticPMID:18818364
overexpression lethal toclr4histone H3 lysine methyltransferase Clr4 Dosage LethalityPMID:16540522
overexpression causes growth defect tocnp1centromere-specific histone H3 CENP-A Dosage Growth DefectPMID:25298518
negative genetic interaction withddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:18818364
negative genetic interaction witherp2COPII-coated vesicle component Erp2/3/4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhht2histone H3 h3.2 Negative GeneticPMID:18818364
negative genetic interaction withilv1acetolactate synthase catalytic subunit Negative GeneticPMID:22681890
negative genetic interaction withmcl1DNA polymerase alpha accessory factor Mcl1 Negative GeneticPMID:22681890
positive genetic interaction withmed20mediator complex subunit Med20 Positive GeneticPMID:18818364
negative genetic interaction withpar1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:18818364
negative genetic interaction withpht1histone H2A variant H2A.Z, Pht1 Negative GeneticPMID:22681890
negative genetic interaction withrdp1RNA-directed RNA polymerase Rdp1 Negative GeneticPMID:18818364
negative genetic interaction withrhn1RNA polymerase II transcription termination factor homolog Negative GeneticPMID:18818364
rescuesrpt319S proteasome regulatory subunit Rpt3 (predicted) Phenotypic SuppressionPMID:24710126
negative genetic interaction withSPCC18.13tRNA (guanine-N7-)-methyltransferase subunit Trm82 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withswd1Set1C complex subunit Swd1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC1834.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1834.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1834.04 BioGRID Interaction Datasets
Expression Viewer SPAC1834.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1834.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1834.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1834.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1834.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1834.04 Transcriptome Viewer (Bähler Lab)
GEO SPAC1834.04 GEO profiles
PInt SPAC1834.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1834.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1834.04 Fission yeast phenotypic data & analysis
Cyclebase SPAC1834.04.1 Cell Cycle Data
SPD / RIKEN03/03B12Orfeome Localization Data
UniProtKB/SwissProtP09988Histone H3.1/H3.2
ModBaseP09988Database of comparative protein structure models
STRINGP09988Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594683histone H3 h3.1
RefSeq mRNANM_001020112972h- histone H3 h3.1 (hht1), mRNA
ePDB2DZEThe European PDB
PDB2DZEPDB
PDBsum2DZEPDBsum
ePDB3G7LThe European PDB
PDB3G7LPDB
PDBsum3G7LPDBsum
European Nucleotide ArchiveBAA21441.1ENA Protein Mapping
European Nucleotide ArchiveCAA17819.1ENA Protein Mapping
European Nucleotide ArchiveCAA28851.1ENA Protein Mapping
European Nucleotide ArchiveCAA28852.1ENA Protein Mapping
European Nucleotide ArchiveCAB75772.1ENA Protein Mapping
UniParcUPI0000162096UniProt Archive

Literature for hht1

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015