hht1 (SPAC1834.04)


Gene Standard Namehht1 Characterisation Statuspublished
Systematic IDSPAC1834.04 Feature Typeprotein coding
Synonyms Name Description
Producthistone H3 h3.1 Product Size136aa, 15.36 kDa
Genomic Location Chromosome I, 4699691-4700560 (870nt); CDS:4699829-4700239 (411nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
DNA binding373
Annotation ExtensionEvidenceWith/FromReference
protein binding862
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
cellular response to DNA damage stimulus212
Annotation ExtensionEvidenceWith/FromReference
chromatin assembly or disassembly71
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
mating-type region heterochromatin28
Annotation ExtensionEvidenceWith/FromReference
nuclear nucleosome10
Annotation ExtensionEvidenceWith/FromReference
nuclear pericentric heterochromatin42
Annotation ExtensionEvidenceWith/FromReference
nuclear subtelomeric heterochromatin21
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
increased viability upon nitrogen starvationhht1Δ54
sensitive to DNA damaging agentsK56R (K56R)7
sensitive to ionizing radiation during vegetative growthK56R (K56R)67
viable vegetative cell populationhht1Δ3831

Cell Phenotype

Term NameGenotypesCount
increased minichromosome loss during vegetative growthhht1+64
viable vegetative cell with normal cell morphologyhht1Δ3099

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased cell population growth at high temperatureS86A,S87A (S86A, S87A), hht3Δ, hht1Δ
S86E (S86E), hht3Δ, hht1Δ
S86A (S86A), hht3Δ, hht1Δ
decreased vegetative cell population growthhta1-S121A (S121A), hht3-T3A (T3A), hta2-S121A (S121A), hht2-T3A (T3A), hht1-T3A (T3A)
loss of viability at high temperaturehht3Δ, S86A,S87A (S86A, S87A), hht1Δ
normal septation indexS86A,S87A (S86A, S87A), hht3Δ, hht1Δ
sensitive to vorinostatS86A,S87A (S86A, S87A), hht3Δ, hht1Δ
slow vegetative cell population growthS86A,S87A (S86A, S87A), hht3Δ, hht1Δ
viable vegetative cell populationT80A (T80A), hht3Δ, hht1Δ

Cell Phenotype

Term NameGenotypes
abolished protein localization to centromere
affecting ark1hta1-S121A (S121A), hht3-T3A (T3A), hta2-S121A (S121A), hht2-T3A (T3A), hht1-T3A (T3A)
affecting ark1hht1-T3A (T3A), hht3-T3A (T3A), hht2-T3A (T3A), sgo2Δ
decreased chromatin binding at centromere central core
affecting cnp1S86A,S87A (S86A, S87A), hht1Δ, hht3Δ, cnp1-1 (L87Q)
decreased protein level during vegetative growth
affecting ams2S86A,S87A (S86A, S87A), hht3Δ, hht1Δ
decreased protein localization to centromere
affecting ark1hht1-T3A (T3A), hht2-T3A (T3A), hht3-T3A (T3A)
decreased RNA level during vegetative growth
affecting ams2S86A,S87A (S86A, S87A), hht3Δ, hht1Δ
increased centromeric transcript levelS86A,S87A (S86A, S87A), hht3Δ, hht1Δ
increased histone H4-K16 acetylation at centromere central coreS86A,S87A (S86A, S87A), hht3Δ, hht1Δ
lagging mitotic chromosomeshht1-T3A (T3A), hht2-T3A (T3A), hht3-T3A (T3A)
hta1-S121A (S121A), hht3-T3A (T3A), hta2-S121A (S121A), hht2-T3A (T3A), hht1-T3A (T3A)
hta2-S121A (S121A), hta1-S121A (S121A), bir1-CD (bir1 C-terminus fused to chromodomain), hht3-T3A (T3A), hht2-T3A (T3A), hht1-T3A (T3A)
hht1-T3A (T3A), hht3-T3A (T3A), hht2-T3A (T3A), sgo2Δ
large and small daughter nucleiS86E (S86E), hht3Δ, hht1Δ
S86A (S86A), hht3Δ, hht1Δ
S86A,S87A (S86A, S87A), hht3Δ, hht1Δ
normal cellular histone levelhht3Δ, T80A (T80A), hht1Δ
hht3Δ, S86A,S87A (S86A, S87A), hht1Δ
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in ams2 cell cycle regulated GATA-type transcription factor Ams2
FYPO affected by mutation in hip1 hira protein, histone chaperone Hip1
FYPO affected by mutation in hsk1 Dbf4(Dfp1)-dependent protein kinase Hsk1
FYPO affected by mutation in mis18 kinetochore protein Mis18
FYPO affected by mutation in scm3 CENP-A chaperone Scm3
FYPO affected by mutation in sim3 NASP family CENP-A chaperone
FYPO affected by mutation in slm9 hira protein Slm9
GO regulated by ams2 cell cycle regulated GATA-type transcription factor Ams2
GO substrate of ark1 aurora-B kinase Ark1
GO substrate of hrk1 haspin related kinase Hrk1
GO substrate of rtt109 RTT109 family histone lysine acetyltransferase
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4699691..4700560
5' UTR4699691..4699828PMID:21511999
3' UTR4700240..4700560SPC03596
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00125 Pfam IPR007125 Histone H2A/H2B/H3 1 132 8
SM00428 SMART IPR000164 Histone H3/CENP-A 34 136 4
PS00322 Prosite Patterns IPR000164 Histone H3/CENP-A 15 21 3
PS00959 Prosite Patterns IPR000164 Histone H3/CENP-A 67 75 3
PTHR11426 HMMPANTHER IPR000164 Histone H3/CENP-A 1 135 4
1.10.20.10 Gene3D IPR009072 Histone-fold 2 134 27
SSF47113 SuperFamily IPR009072 Histone-fold 2 133 26
PR00622 PRINTS IPR000164 Histone H3/CENP-A 3 17 4
PR00622 PRINTS IPR000164 Histone H3/CENP-A 17 31 4
PR00622 PRINTS IPR000164 Histone H3/CENP-A 34 55 4
PR00622 PRINTS IPR000164 Histone H3/CENP-A 58 75 4
PR00622 PRINTS IPR000164 Histone H3/CENP-A 80 98 4
PR00622 PRINTS IPR000164 Histone H3/CENP-A 98 114 4
PR00622 PRINTS IPR000164 Histone H3/CENP-A 114 135 4

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000297histone foldTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000297

Protein Properties

Ave. residue weight 112.92 Da
Charge 21.00
Codon Adaptation Index 0.72
Isoelectric point 11.83
Molecular weight 15.36 kDa
Number of residues 136
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS10 1675
Annotation ExtensionEvidenceResidueReference
experimental evidence S10 PMID:24763107
N6-acetyl-L-lysineK56 4
Annotation ExtensionEvidenceResidueReference
IDA K56 PMID:17369611
acetylated residue 14
Annotation ExtensionEvidenceResidueReference
ISS PB_REF:0000001
methylated residue 18
present during chromatin silencing at centromereK9
present during chromatin silencing at centromereK14
K4
Annotation ExtensionEvidenceResidueReference
ISS K4 PB_REF:0000001
present during chromatin silencing at centromere NAS K9 PMID:14561399
present during chromatin silencing at centromere NAS K14 PMID:14561399
methylated lysine 7
Annotation ExtensionEvidenceResidueReference
IDA PMID:18292091
N-acetylated L-lysine 4
added by mst1K4
added during mitotic S phaseK4
K9
added during mitotic S phaseK56
Annotation ExtensionEvidenceResidueReference
IDA PMID:18292091
added during mitotic S phase IDA K4 PMID:20299449
added by mst1 IDA K4 PMID:20299449
IDA K9 PMID:20299449
added during mitotic S phase IDA K56 PMID:20299449
ubiquitinylated lysineK36 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K36 PMID:26412298
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for hht1 (SPAC1834.04)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5289during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5036during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
6570during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5075during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5429during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
28during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3422
conserved in vertebrates3397
conserved in eukaryotes only2504
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPAC1834.04 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withxap5xap-5-like protein Negative GeneticPMID:24957674
negative genetic interaction withmis4adherin, cohesin loading factor Mis4 Negative GeneticPMID:23050226
External References
Database Identifier Description
NBRP SPAC1834.04 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1834.04 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1834.04 BioGRID Interaction Datasets
Expression Viewer SPAC1834.04 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1834.04 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1834.04 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1834.04 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1834.04 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1834.04 Transcriptome Viewer (Bähler Lab)
GEO SPAC1834.04 GEO profiles
PInt SPAC1834.04 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1834.04 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1834.04 Fission yeast phenotypic data & analysis
Cyclebase SPAC1834.04.1 Cell Cycle Data
SPD / RIKEN03/03B12Orfeome Localization Data
UniProtKB/SwissProtP09988Histone H3.1/H3.2
ModBaseP09988Database of comparative protein structure models
STRINGP09988Network display of known and predicted interactions and functional associations
UniProtKB/SwissProtP10651Histone H3.3
ModBaseP10651Database of comparative protein structure models
STRINGP10651Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594683histone H3 h3.1
RefSeq mRNANM_001020112972h- histone H3 h3.1 (hht1), mRNA
European Nucleotide ArchiveAB004538ENA EMBL mapping
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveCU329671ENA EMBL mapping
European Nucleotide ArchiveX05222ENA EMBL mapping
European Nucleotide ArchiveX05223ENA EMBL mapping
European Nucleotide ArchiveX05224ENA EMBL mapping
ePDB2DZEThe European PDB
PDB2DZEPDB
PDBsum2DZEPDBsum
ePDB3G7LThe European PDB
PDB3G7LPDB
PDBsum3G7LPDBsum
European Nucleotide ArchiveBAA21441ENA Protein Mapping
European Nucleotide ArchiveCAA17819ENA Protein Mapping
European Nucleotide ArchiveCAA28851ENA Protein Mapping
European Nucleotide ArchiveCAA28852ENA Protein Mapping
European Nucleotide ArchiveCAA28854ENA Protein Mapping
European Nucleotide ArchiveCAB50974ENA Protein Mapping
European Nucleotide ArchiveCAB75772ENA Protein Mapping
UniParcUPI0000162096UniProt Archive

Literature for hht1

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016