alg9 (SPAC1834.05)


Gene Standard Namealg9 Characterisation Statusbiological role inferred
Systematic IDSPAC1834.05 Feature Typeprotein coding
Synonyms Name Description
Productmannosyltransferase complex subunit Alg9 (predicted) Product Size577aa, 66.27 kDa
Genomic Location Chromosome I, 4702256-4704743 (2488nt); CDS:4702588-4704321 (1734nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
dolichol-linked oligosaccharide biosynthetic process8
Annotation ExtensionEvidenceWith/FromReference
protein N-linked glycosylation33
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum599
Annotation ExtensionEvidenceWith/FromReference
integral component of endoplasmic reticulum membrane68
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
resistance to hydrogen peroxidealg9Δ75
sensitive to 5-fluorouracilalg9Δ307
sensitive to caffeine during vegetative growthalg9Δ110
sensitive to hydrazinocurcuminalg9+/alg9- (heterozygous diploid)8
viable vegetative cell populationalg9Δ3850

Cell Phenotype

Term NameGenotypesCount
inviable vegetative cell with normal cell morphologyalg9Δ66
viable vegetative cell with normal cell morphologyalg9Δ3102
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4702256..4704743
mRNA4702256..4704743
5' UTR4702256..4702587PMID:21511999
CDS4702588..4704321
3' UTR4704322..4704743PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03901 Pfam IPR005599 GPI mannosyltransferase 16 438 4
TMhelix TMHMM 12 34 950
TMhelix TMHMM 66 88 950
TMhelix TMHMM 374 396 950
TMhelix TMHMM 339 361 950
TMhelix TMHMM 176 198 950
TMhelix TMHMM 307 326 950
TMhelix TMHMM 218 240 950
TMhelix TMHMM 137 159 950
TMhelix TMHMM 95 117 950
TMhelix TMHMM 280 302 950
PTHR22760:SF2 HMMPANTHER 3 568 1
PTHR22760 HMMPANTHER IPR005599 GPI mannosyltransferase 3 568 4
SignalP-noTM signalp 1 29 207

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0000418signal_peptide279
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 114.85 Da
Charge 10.00
Codon Adaptation Index 0.44
Isoelectric point 8.31
Molecular weight 66.27 kDa
Number of residues 577
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for alg9 (SPAC1834.05)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
19760during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
20250during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
19254during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
20835during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
3189.79during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
20244during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5916.62during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.1during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.52during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
congenital disorders of glycosylation30
metabolic disorders143
fatty acid metabolism disorders21
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC1834.05 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withsds23PP2A-type phosphatase inhibitor Sds23/Moc1 Two-hybridPMID:19682301
binds DNA-binding domain construct withmoc3transcription factor Moc3 Two-hybridPMID:19682301
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC1834.05 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withski3Ski complex TPR repeat subunit Ski3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgma12alpha-1,2-galactosyltransferase Gma12 Negative GeneticPMID:22681890
negative genetic interaction withgid9GID complex subunit Gid9 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withspa2cell polarity protein Spa2 Negative GeneticPMID:22681890
negative genetic interaction withcsn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:22681890
negative genetic interaction withSPBC17A3.03cphosphoprotein phosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withatb2tubulin alpha 2 Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withubp8SAGA complex ubiquitin C-terminal hydrolase Ubp8 Negative GeneticPMID:22681890
negative genetic interaction withrec14Ski complex subunit Rec14 Negative GeneticPMID:22681890
negative genetic interaction withubc6ubiquitin conjugating enzyme E2 Ubc6 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withlsc1Lsk1 associated cyclin Negative GeneticPMID:22681890
negative genetic interaction withngg1SAGA complex subunit Ngg1/Ada3 Negative GeneticPMID:22681890
negative genetic interaction withsub1transcription coactivator PC4 Negative GeneticPMID:22681890
negative genetic interaction withpck1protein kinase C (PKC)-like Pck1 Negative GeneticPMID:22681890
negative genetic interaction withssp2AMP-activated protein serine/threonine kinase alpha subunit Ssp2 Negative GeneticPMID:22681890
negative genetic interaction withgmh2alpha-1,2-galactosyltransferase Gmh2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
negative genetic interaction withint6eIF3e subunit Int6 Negative GeneticPMID:22681890
negative genetic interaction withnup61nucleoporin Nup61 Negative GeneticPMID:22681890
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
negative genetic interaction withhrp3ATP-dependent DNA helicase Hrp3 Negative GeneticPMID:22681890
negative genetic interaction withsgf29SAGA complex subunit Sgf29 Negative GeneticPMID:22681890
negative genetic interaction withvph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
negative genetic interaction witheso1mitotic cohesin N-acetyltransferase/DNA polymerase eta Eso1 fusion protein Negative GeneticPMID:23050226
negative genetic interaction withpom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
positive genetic interaction withatg6autophagy associated beclin family protein Atg6 Positive GeneticPMID:22681890
positive genetic interaction withufd2ubiquitin-protein ligase E4 Ufd2 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrpa12DNA-directed RNA polymerase complex I subunit Rpa12 Positive GeneticPMID:22681890
positive genetic interaction withtor1serine/threonine protein kinase Tor1 Positive GeneticPMID:22681890
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withmak3histidine kinase Mak3 Positive GeneticPMID:22681890
positive genetic interaction withtop1DNA topoisomerase I Positive GeneticPMID:22681890
positive genetic interaction withace2transcription factor Ace2 Positive GeneticPMID:22681890
positive genetic interaction withswc5Swr1 complex subunit Swc5 Positive GeneticPMID:22681890
positive genetic interaction withdot2ESCRT II complex subunit Dot2 Positive GeneticPMID:22681890
positive genetic interaction withsts5RNB-like protein Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC1834.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1834.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1834.05 BioGRID Interaction Datasets
Expression Viewer SPAC1834.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1834.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1834.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1834.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1834.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1834.05 Transcriptome Viewer (Bähler Lab)
GEO SPAC1834.05 GEO profiles
PInt SPAC1834.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1834.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1834.05 Fission yeast phenotypic data & analysis
Cyclebase SPAC1834.05.1 Cell Cycle Data
SPD / RIKEN35/35G06Orfeome Localization Data
UniProtKB/SwissProtQ9P7Q9Alpha-1,2-mannosyltransferase alg9
ModBaseQ9P7Q9Database of comparative protein structure models
STRINGQ9P7Q9Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594684mannosyltransferase complex subunit Alg9 (predicted)
RefSeq mRNANM_001020113972h- mannosyltransferase complex subunit Alg9 (predicted) (alg9), mRNA
European Nucleotide ArchiveCAB75773.1ENA Protein Mapping
UniParcUPI000013AEFCUniProt Archive

Literature for alg9

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016