sec18 (SPAC1834.11c)


Gene Standard Namesec18 Characterisation Statusbiological role inferred
Systematic IDSPAC1834.11c Feature Typeprotein coding
Synonyms Name Description
Productsecretory pathway protein Sec18 (predicted) Product Size792aa, 87.55 kDa
Genomic Location Chromosome I, 4721597-4718513 (3085nt); CDS:4721116-4718738 (2379nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding557
Annotation ExtensionEvidenceWith/FromReference
ATPase activity238
Annotation ExtensionEvidenceWith/FromReference
protein binding887
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ER to Golgi vesicle-mediated transport74
Annotation ExtensionEvidenceWith/FromReference
intracellular protein transport316
Annotation ExtensionEvidenceWith/FromReference
vacuole fusion, non-autophagic20
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationsec18Δ1455

Cell Phenotype

Term NameGenotypesCount
inviable after spore germination with normal, unseptated germ tube morphologysec18Δ237
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4721597..4718513
mRNA4721597..4718513
5' UTR4721597..4721117PMID:21511999
CDS4721116..4718738
3' UTR4718737..4718513PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00004 Pfam IPR003959 ATPase, AAA-type, core 588 717 32
PF00004 Pfam IPR003959 ATPase, AAA-type, core 307 447 32
PF02933 Pfam IPR004201 CDC48, domain 2 163 217 2
SM01072 SMART IPR004201 CDC48, domain 2 163 235 2
SM00382 SMART IPR003593 AAA+ ATPase domain 303 450 70
SM00382 SMART IPR003593 AAA+ ATPase domain 584 720 70
SM01073 SMART IPR003338 CDC48, N-terminal subdomain 60 136 2
PS00674 Prosite Patterns IPR003960 ATPase, AAA-type, conserved site 418 436 19
PTHR23078:SF3 HMMPANTHER 50 774 1
PTHR23078 HMMPANTHER 50 774 1
1.10.8.60 Gene3D Proteasome-activating nucleotidase PAN 458 539 35
2.40.40.20 Gene3D CDC48, N-terminal subdomain 60 133 5
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 271 457 288
3.10.330.10 Gene3D IPR029067 CDC48 domain 2-like 141 247 3
3.40.50.300 Gene3D IPR027417 P-loop containing nucleoside triphosphate hydrolase 563 716 288
SSF50692 SuperFamily IPR009010 Aspartate decarboxylase-like domain 60 136 2
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 547 769 294
SSF54585 SuperFamily IPR029067 CDC48 domain 2-like 139 252 3
SSF52540 SuperFamily IPR027417 P-loop containing nucleoside triphosphate hydrolase 271 548 294

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000539AAA family ATPaseTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000539

Protein Properties

Ave. residue weight 110.55 Da
Charge 1.00
Codon Adaptation Index 0.47
Isoelectric point 6.62
Molecular weight 87.55 kDa
Number of residues 792
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
present during mitotic M phase, cellular response to thiabendazoleS256
present during cellular response to thiabendazoleS58
S58, S256, S488
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S256 PMID:21712547
present during cellular response to thiabendazole IDA S256 PMID:18257517
present during cellular response to thiabendazole IDA S58 PMID:18257517
IDA S58 PMID:25720772
IDA S488 PMID:25720772
IDA S256 PMID:25720772
O-phospho-L-threonineT252 1085
Annotation ExtensionEvidenceResidueReference
IDA T252 PMID:25720772
ubiquitinylated lysineK171 512
Annotation ExtensionEvidenceResidueReference
mass spectrometry evidence K171 PMID:26412298
IDA PMID:26412298
O-phosphorylated residueS250,S256, S250,T252 2457
Annotation ExtensionEvidenceResidueReference
IDA S250,T252 PMID:25720772
IDA S250,S256 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for sec18 (SPAC1834.11c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
39217during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
38595during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
41334during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
41613during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
40986during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
10358.74during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
14045.73during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4.8during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC1834.11c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withmug178mitochondrial ribosomal protein subunit L51-b (predicted) Two-hybridPMID:26771498
binds activation domain construct withimp1importin alpha Two-hybridPMID:26771498
binds activation domain construct withmmi1YTH family RNA binding protein Mmi1 Two-hybridPMID:26771498
binds activation domain construct withatg11autophagy protein Atg11 Two-hybridPMID:26771498
binds activation domain construct withmrpl9mitochondrial ribosomal protein subunit L9 (predicted) Two-hybridPMID:26771498
binds activation domain construct withtif222translation initiation factor eIF2B beta subunit Two-hybridPMID:26771498
binds activation domain construct withrcd1RNA-binding protein, CCR4-NOT complex subunit Rcd1 Two-hybridPMID:23695164
binds activation domain construct withisu1mitochondrial iron-sulfur cluster assembly scaffold protein Isu1 Two-hybridPMID:26771498
binds activation domain construct withcsl4exosome subunit Csl4 Two-hybridPMID:26771498
binds activation domain construct withrpl80360S ribosomal protein L8 (predicted) Two-hybridPMID:26771498
binds activation domain construct withcdc25M phase inducer tyrosine phosphatase Cdc25 Two-hybridPMID:26771498
binds activation domain construct withmss51mitochondrial splicing suppressor Mss51 (predicted) Two-hybridPMID:26771498
binds activation domain construct withSPCC1739.06curoporphyrin methyltransferase (predicted) Two-hybridPMID:26771498
binds activation domain construct withatp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Two-hybridPMID:26771498
binds activation domain construct withsec18secretory pathway protein Sec18 (predicted) Two-hybridPMID:23695164
binds activation domain construct withmat3-Mcmating-type m-specific HMG-box transcription factor Mc at silenced MAT3 locus Two-hybridPMID:26771498
binds activation domain construct withlaf2Clr6 associated factor 2, Laf2 Two-hybridPMID:26771498
binds activation domain construct withmug24RNA-binding protein, rrm type Two-hybridPMID:26771498
binds DNA-binding domain construct withcyc2mitochondrial cytochrome c-heme linkage protein Cyc2 (predicted) Two-hybridPMID:26771498
binds DNA-binding domain construct withpre920S proteasome complex subunit alpha 3 Pre9 (predicted) Two-hybridPMID:26771498
binds DNA-binding domain construct withmst1KAT5 family histone acetyltransferase Mst1 Two-hybridPMID:18505873
External References
Database Identifier Description
NBRP SPAC1834.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1834.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1834.11c BioGRID Interaction Datasets
Expression Viewer SPAC1834.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1834.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1834.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1834.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1834.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1834.11c Transcriptome Viewer (Bähler Lab)
GEO SPAC1834.11c GEO profiles
PInt SPAC1834.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1834.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1834.11c Fission yeast phenotypic data & analysis
Cyclebase SPAC1834.11c.1 Cell Cycle Data
SPD / RIKEN28/28E07Orfeome Localization Data
UniProtKB/SwissProtQ9P7Q4Vesicular-fusion protein sec18
ModBaseQ9P7Q4Database of comparative protein structure models
STRINGQ9P7Q4Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594690secretory pathway protein Sec18 (predicted)
RefSeq mRNANM_001020119972h- secretory pathway protein Sec18 (predicted) (sec18), mRNA
European Nucleotide ArchiveCAB75779.1ENA Protein Mapping
UniParcUPI00001355CCUniProt Archive

Literature for sec18

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016