mra1 (SPAC18G6.07c)


Gene Standard Namemra1 Characterisation Statuspublished
Systematic IDSPAC18G6.07c Feature Typeprotein coding
Synonyms Name DescriptionMulticopy supressor of RAs
ProductrRNA (pseudouridine) methyltransferase Mra1 Product Size359aa, 39.78 kDa
Genomic Location Chromosome I, 2231496-2229963 (1534nt); CDS:2231412-2230333 (1080nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process

GO Slim Terms

ribosome biogenesis


Term NameCount
response to pheromone36
Annotation ExtensionEvidenceWith/FromReference
ribosomal small subunit biogenesis54
Annotation ExtensionEvidenceWith/FromReference
rRNA base methylation1
Annotation ExtensionEvidenceWith/FromReference
rRNA methylation17
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
nucleus2740
Annotation ExtensionEvidenceWith/FromReference
small-subunit processome36
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaymra1-12 (R127L, H200R)OverexpressionPMID:9133664147
Cell growth assaymra1-12b (H200R)OverexpressionPMID:9133664
FYPO:0002061inviable vegetative cell populationCell growth assaymra1ΔNullPMID:91336641338
Microscopymra1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Microscopymra1ΔNullPMID:20473289
FYPO:0001164normal growth on glucose carbon sourceCell growth assaymra1-12a (R127L)OverexpressionPMID:9133664176
Cell growth assaymra1-3 (K213E, I358T)OverexpressionPMID:9133664
Cell growth assaymra1-1 (T223P)OverexpressionPMID:9133664

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000054abnormal microtubule cytoskeleton organizationMicroscopynot recorded (overexpression)PMID:15797925266
FYPO:0000059abnormal mitotic cell cycleMicroscopymra1ΔNullPECO:0000005, PECO:0000137PMID:23697806686
FYPO:0000708decreased mating efficiencyCell growth assaymra1-3 (K213E, I358T)OverexpressionPMID:9133664240
Cell growth assaymra1-1 (T223P)OverexpressionPMID:9133664
FYPO:0002430inviable after spore germination, multiple cell divisionsMicroscopymra1ΔNullPMID:9133664210
FYPO:0002379inviable after spore germination, without cell division, with elongated germ tube58
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
Microscopymra1ΔNullPECO:0000137, PECO:0000005PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
122314962229963

UTRs

Region Coordinates Reference
five_prime_UTR2231496..2231413PMID:21511999
three_prime_UTR2230332..2229963PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03587 Pfam IPR005304 Ribosomal biogenesis, methyltransferase, EMG1/NEP1 152 353 1
PTHR12636 HMMPANTHER IPR005304 Ribosomal biogenesis, methyltransferase, EMG1/NEP1 85 359 1
PTHR12636:SF4 HMMPANTHER 85 359 1
SSF75217 SuperFamily Alpha/beta knot methyltransferases 141 358 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.80 Da
Charge 7.50
Isoelectric point 8.36
Molecular weight 39.78 kDa
Number of residues 359
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS12PMID:247631071670
experimental evidenceS100PMID:24763107
present during mitotic M phaseexperimental evidenceS16PMID:21712547
present during mitotic M phaseexperimental evidenceS30PMID:21712547
present during cellular response to thiabendazoleIDAS57PMID:18257517
present during mitotic M phaseexperimental evidenceS31PMID:21712547
present during mitotic M phaseexperimental evidenceS36PMID:21712547
present during mitotic M phaseexperimental evidenceS100PMID:21712547
present during mitotic M phaseexperimental evidenceS82PMID:21712547
added by ark1, present during mitotic M phaseexperimental evidenceS12PMID:21712547
present during cellular response to thiabendazoleIDAS100PMID:18257517
present during mitotic M phaseexperimental evidenceS28PMID:21712547
added by ark1, present during mitotic M phaseexperimental evidenceS9PMID:21712547
MOD:00047O-phospho-L-threonineexperimental evidenceT33PMID:21712547692
present during mitotic M phase
MOD:00696phosphorylated residueNASPMID:195477441922
NASPMID:18257517
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2272during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2332during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
2215during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1787during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
1909during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
9531.97during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
5612.66during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.1during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.93during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
Bowen Conradi syndrome1
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
ark1aurora-B kinase Ark1 Biochemical ActivityPMID:21712547
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
zfs1CCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism Phenotypic SuppressionPMID:9133664
gap1GTPase activating protein Gap1 Phenotypic SuppressionPMID:9133664
ras1GTPase Ras1 Phenotypic SuppressionPMID:9133664
External References
Database Identifier Description
NBRP SPAC18G6.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC18G6.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC18G6.07c BioGRID Interaction Datasets
Expression Viewer SPAC18G6.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC18G6.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC18G6.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC18G6.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC18G6.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC18G6.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC18G6.07c Cell Cycle Data
GEO SPAC18G6.07c GEO profiles
PInt SPAC18G6.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC18G6.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC18G6.07c Fission yeast phenotypic data & analysis
SPD / RIKEN16/16G05Orfeome Localization Data
UniProtKB/SwissProtQ10107Ribosomal RNA small subunit methyltransferase mra1
ModBaseQ10107Database of comparative protein structure models
STRINGQ10107Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593671ribosome biogenesis protein Mra1
RefSeq mRNANM_001019103972h- ribosome biogenesis protein Mra1 (mra1), mRNA
European Nucleotide ArchiveBAA24497.1ENA Protein Mapping
European Nucleotide ArchiveCAA92394.1ENA Protein Mapping
UniParcUPI000012F4D7UniProt Archive

Literature for mra1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014