mra1 (SPAC18G6.07c)


Gene Standard Namemra1 Characterisation Statuspublished
Systematic IDSPAC18G6.07c Feature Typeprotein coding
Synonyms Name DescriptionMulticopy supressor of RAs
ProductrRNA (pseudouridine) methyltransferase Mra1 Product Size359aa, 39.78 kDa
Genomic Location Chromosome I, 2231496-2229963 (1534nt); CDS:2231412-2230333 (1080nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process

GO Slim Terms

ribosome biogenesis


Term NameCount
response to pheromone34
Annotation ExtensionEvidenceWith/FromReference
ribosomal small subunit biogenesis55
Annotation ExtensionEvidenceWith/FromReference
rRNA base methylation1
Annotation ExtensionEvidenceWith/FromReference
rRNA methylation18
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2316
Annotation ExtensionEvidenceWith/FromReference
nucleolus359
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
small-subunit processome36
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth on glucose carbon sourcemra1-12 (R127L, H200R)Overexpression154
mra1-12b (H200R)Overexpression
decreased mating efficiencymra1-1 (T223P)Overexpression247
mra1-3 (K213E, I358T)Overexpression
inviable vegetative cell populationmra1ΔNull1427
normal growth on glucose carbon sourcemra1-1 (T223P)Overexpression189
mra1-12a (R127L)Overexpression
mra1-3 (K213E, I358T)Overexpression

Cell Phenotype

Term NameAlleleExpressionCount
abnormal microtubule cytoskeleton organization during vegetative growthnot recorded (overexpression)59
abnormal mitotic cell cyclemra1ΔNull794
inviable after spore germination, multiple cell divisionsmra1ΔNull221
inviable after spore germination, without cell division, with elongated germ tube62
penetrance FYPO_EXT:0000001, expressivity FYPO_EXT:0000003mra1ΔNull
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
122314962229963

UTRs

Region Coordinates Reference
five_prime_UTR2231496..2231413PMID:21511999
three_prime_UTR2230332..2229963PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03587 Pfam IPR005304 Ribosomal biogenesis, methyltransferase, EMG1/NEP1 152 353 1
PTHR12636:SF5 HMMPANTHER 81 359 1
PTHR12636 HMMPANTHER IPR005304 Ribosomal biogenesis, methyltransferase, EMG1/NEP1 81 359 1
SSF75217 SuperFamily IPR029028 Alpha/beta knot methyltransferases 141 358 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.80 Da
Charge 7.50
Isoelectric point 8.36
Molecular weight 39.78 kDa
Number of residues 359
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS31
present during mitotic M phaseS16
S12, S100
present during mitotic M phaseS36
added by ark1, present during mitotic M phaseS12
present during mitotic M phase, cellular response to thiabendazoleS100
present during mitotic M phaseS28
present during mitotic M phaseS82
present during cellular response to thiabendazoleS57
added by ark1, present during mitotic M phaseS9
present during mitotic M phaseS30
Annotation ExtensionEvidenceResidueReference
added by ark1
present during mitotic M phase
experimental evidence S9 PMID:21712547
experimental evidence S12 PMID:24763107
added by ark1
present during mitotic M phase
experimental evidence S12 PMID:21712547
present during mitotic M phase experimental evidence S16 PMID:21712547
present during mitotic M phase experimental evidence S28 PMID:21712547
present during mitotic M phase experimental evidence S30 PMID:21712547
present during mitotic M phase experimental evidence S31 PMID:21712547
present during mitotic M phase experimental evidence S36 PMID:21712547
present during cellular response to thiabendazole IDA S57 PMID:18257517
present during mitotic M phase experimental evidence S82 PMID:21712547
experimental evidence S100 PMID:24763107
present during mitotic M phase experimental evidence S100 PMID:21712547
present during cellular response to thiabendazole IDA S100 PMID:18257517
O-phospho-L-threonine 693
present during mitotic M phaseT33
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T33 PMID:21712547
phosphorylated residue 1922
Annotation ExtensionEvidenceResidueReference
NAS PMID:18257517
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2272during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2332during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2215during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1787during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1909during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
9531.97during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
5612.66during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.1during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.93during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
Bowen Conradi syndrome1
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
ark1aurora-B kinase Ark1 Biochemical ActivityPMID:21712547
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
zfs1CCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism Phenotypic SuppressionPMID:9133664
gap1GTPase activating protein Gap1 Phenotypic SuppressionPMID:9133664
ras1GTPase Ras1 Phenotypic SuppressionPMID:9133664
External References
Database Identifier Description
NBRP SPAC18G6.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC18G6.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC18G6.07c BioGRID Interaction Datasets
Expression Viewer SPAC18G6.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC18G6.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC18G6.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC18G6.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC18G6.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC18G6.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC18G6.07c Cell Cycle Data
GEO SPAC18G6.07c GEO profiles
PInt SPAC18G6.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC18G6.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC18G6.07c Fission yeast phenotypic data & analysis
SPD / RIKEN16/16G05Orfeome Localization Data
UniProtKB/SwissProtQ10107Ribosomal RNA small subunit methyltransferase mra1
ModBaseQ10107Database of comparative protein structure models
STRINGQ10107Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593671ribosome biogenesis protein Mra1
RefSeq mRNANM_001019103972h- ribosome biogenesis protein Mra1 (mra1), mRNA
European Nucleotide ArchiveBAA24497.1ENA Protein Mapping
European Nucleotide ArchiveCAA92394.1ENA Protein Mapping
UniParcUPI000012F4D7UniProt Archive

Literature for mra1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015