mra1 (SPAC18G6.07c)


Gene Standard Namemra1 Characterisation Statuspublished
Systematic IDSPAC18G6.07c Feature Typeprotein coding
Synonyms Name Description
ProductrRNA (pseudouridine) methyltransferase Mra1 Product Size359aa, 39.78 kDa
Genomic Location Chromosome I, 2231496-2229963 (1534nt); CDS:2231412-2230333 (1080nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0070037rRNA (pseudouridine) methyltransferase activityISSUniProtKB:Q92979GO_REF:00000241
ISOSGD:S000004176GO_REF:0000024
GO:0019843rRNA bindingIEAUniProtKB-KW:KW-0699GO_REF:000003746
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0070475rRNA base methylationISOSGD:S000004176GO_REF:00000241
GO:0031167rRNA methylationISSUniProtKB:Q92979GO_REF:000002416
GO:0019236response to pheromoneIEAUniProtKB-KW:KW-0589GO_REF:000003730
GO:0042274ribosomal small subunit biogenesisISOSGD:S000004176GO_REF:000002452
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722316
GO:0005730nucleolusIEASL-0188GO_REF:0000039355
GO:0005634nucleusIDAPMID:168233722740
GO:0032040small-subunit processomeISOSGD:S000004176GO_REF:000002435
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopymra1ΔNullPECO:0000005, PECO:0000137PMID:236978061315
not recorded (unrecorded)PMID:9133664
Microscopymra1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000054abnormal microtubule cytoskeleton organizationnot recorded (overexpression)PMID:1579792549
FYPO:0000059abnormal mitotic cell cycleMicroscopymra1ΔNullPECO:0000005, PECO:0000137PMID:23697806624
FYPO:0002379inviable after spore germination, without cell division, with elongated germ tube56
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000001
Microscopymra1ΔNullPECO:0000137, PECO:0000005PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
122314962229963

UTRs

Region Coordinates Reference
five_prime_UTR2231496..2231413PMID:21511999
three_prime_UTR2230332..2229963PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03587 Pfam IPR005304 Ribosomal biogenesis, methyltransferase, EMG1/NEP1 152 353 1
PTHR12636:SF5 HMMPANTHER 81 359 1
PTHR12636 HMMPANTHER IPR005304 Ribosomal biogenesis, methyltransferase, EMG1/NEP1 81 359 1
SSF75217 SuperFamily IPR029028 Alpha/beta knot methyltransferases 141 358 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.80 Da
Charge 7.50
Isoelectric point 8.36
Molecular weight 39.78 kDa
Number of residues 359
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS12PMID:247631071663
present during cellular response to thiabendazoleIDAS100PMID:18257517
present during cellular response to thiabendazoleIDAS57PMID:18257517
present during mitotic M phaseS16PMID:21712547
S100PMID:24763107
present during mitotic M phaseS100PMID:21712547
present during mitotic M phaseS30PMID:21712547
present during mitotic M phaseS31PMID:21712547
present during mitotic M phaseS82PMID:21712547
present during mitotic M phaseS28PMID:21712547
present during mitotic M phaseS36PMID:21712547
added by ark1, present during mitotic M phaseS12PMID:21712547
added by ark1, present during mitotic M phaseS9PMID:21712547
MOD:00047O-phospho-L-threonineT33PMID:21712547682
present during mitotic M phase
MOD:00696phosphorylated residueNASPMID:182575171915
NASPMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
2272during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
2332during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
1909during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
9531.97during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
5612.66during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
1787during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
2215during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.93during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
5.1during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
Bowen Conradi syndrome1
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
zfs1CCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism Phenotypic SuppressionPMID:9133664
gap1GTPase activating protein Gap1 Phenotypic SuppressionPMID:9133664
ras1GTPase Ras1 Phenotypic SuppressionPMID:9133664
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
ark1aurora-B kinase Ark1 Biochemical ActivityPMID:21712547
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPAC18G6.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC18G6.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC18G6.07c BioGRID Interaction Datasets
Expression Viewer SPAC18G6.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC18G6.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC18G6.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC18G6.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC18G6.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC18G6.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC18G6.07c Cell Cycle Data
GEO SPAC18G6.07c GEO profiles
PInt SPAC18G6.07c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542139ribosome biogenesis protein Mra1
EntrezGene2542139ribosome biogenesis protein Mra1
SPD / RIKEN16/16G05Orfeome Localization Data
UniProtKB/SwissProtQ10107Probable ribosomal RNA small subunit methyltransferase mra1
ModBaseQ10107Database of comparative protein structure models
STRINGQ10107Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593671ribosome biogenesis protein Mra1
RefSeq mRNANM_001019103972h- ribosome biogenesis protein Mra1 (mra1), mRNA
European Nucleotide ArchiveBAA24497ENA Protein Mapping
European Nucleotide ArchiveBAA24497.1ENA Protein Mapping
European Nucleotide ArchiveCAA92394ENA Protein Mapping
European Nucleotide ArchiveCAA92394.1ENA Protein Mapping
UniParcUPI000012F4D7UniProt Archive

Literature for mra1

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014