mra1 (SPAC18G6.07c)


Gene Standard Namemra1 Characterisation Statuspublished
Systematic IDSPAC18G6.07c Feature Typeprotein coding
Synonyms Name DescriptionMulticopy supressor of RAs
ProductrRNA (pseudouridine) methyltransferase Mra1 Product Size359aa, 39.78 kDa
Genomic Location Chromosome I, 2231496-2229963 (1534nt); CDS:2231412-2230333 (1080nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process

GO Slim Terms

ribosome biogenesis


Term NameCount
response to pheromone32
Annotation ExtensionEvidenceWith/FromReference
ribosomal small subunit biogenesis67
Annotation ExtensionEvidenceWith/FromReference
rRNA base methylation1
Annotation ExtensionEvidenceWith/FromReference
rRNA methylation16
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
nucleolus365
Annotation ExtensionEvidenceWith/FromReference
nucleus2700
Annotation ExtensionEvidenceWith/FromReference
small-subunit processome36
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth on glucose carbon sourcemra1-12b (H200R)380
mra1-12 (R127L, H200R)
decreased mating efficiencymra1-3 (K213E, I358T)275
mra1-1 (T223P)
inviable vegetative cell populationmra1Δ1455
normal growth on glucose carbon sourcemra1-1 (T223P)294
mra1-12a (R127L)
mra1-3 (K213E, I358T)

Cell Phenotype

Term NameGenotypesCount
abnormal microtubule cytoskeleton organization during vegetative growthmra1+ (wild type)295
abnormal mitotic cell cyclemra1Δ894
inviable after spore germination, multiple cell divisionsmra1Δ243
inviable after spore germination, without cell division, with elongated germ tubemra1Δ66

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased mating efficiencymra1+ (wild type), zfs1Δ
mra1+ (wild type), ras1-S40
gap1+, mra1+ (wild type)
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in ark1 aurora-B kinase Ark1
GO substrate of ark1 aurora-B kinase Ark1
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2231496..2229963
mRNA2231496..2229963
5' UTR2231496..2231413PMID:21511999
CDS2231412..2230333
3' UTR2230332..2229963PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03587 Pfam IPR005304 Ribosomal biogenesis, methyltransferase, EMG1/NEP1 152 353 1
PTHR12636:SF5 HMMPANTHER 99 359 1
PTHR12636 HMMPANTHER IPR005304 Ribosomal biogenesis, methyltransferase, EMG1/NEP1 99 359 1
SSF75217 SuperFamily IPR029028 Alpha/beta knot methyltransferases 141 358 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.80 Da
Charge 7.50
Codon Adaptation Index 0.46
Isoelectric point 8.36
Molecular weight 39.78 kDa
Number of residues 359
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 2289
added by ark1, present during mitotic M phaseS9
added by ark1, present during mitotic M phaseS12
present during mitotic M phaseS36
present during cellular response to thiabendazole, mitotic M phaseS100
present during mitotic M phaseS16
present during cellular response to thiabendazoleS57
present during mitotic M phaseS31
present during mitotic M phaseS30
present during mitotic M phaseS82
present during mitotic M phaseS28
S12, S28, S30, S31, S57, S72, S82, S100, S124, S351
Annotation ExtensionEvidenceResidueReference
added by ark1
present during mitotic M phase
experimental evidence S9 PMID:21712547
added by ark1
present during mitotic M phase
experimental evidence S12 PMID:21712547
present during mitotic M phase experimental evidence S36 PMID:21712547
present during cellular response to thiabendazole IDA S100 PMID:18257517
present during mitotic M phase experimental evidence S16 PMID:21712547
present during cellular response to thiabendazole IDA S57 PMID:18257517
present during mitotic M phase experimental evidence S31 PMID:21712547
present during mitotic M phase experimental evidence S100 PMID:21712547
present during mitotic M phase experimental evidence S30 PMID:21712547
present during mitotic M phase experimental evidence S82 PMID:21712547
present during mitotic M phase experimental evidence S28 PMID:21712547
IDA S30 PMID:25720772
IDA S72 PMID:25720772
IDA S31 PMID:25720772
IDA S82 PMID:25720772
IDA S100 PMID:25720772
IDA S57 PMID:25720772
IDA S28 PMID:25720772
experimental evidence S100 PMID:24763107
IDA S351 PMID:25720772
experimental evidence S12 PMID:24763107
IDA S12 PMID:25720772
IDA S124 PMID:25720772
O-phospho-L-threonine 1085
present during mitotic M phaseT33
T33
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T33 PMID:21712547
IDA T33 PMID:25720772
phosphorylated residue 2511
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
O-phosphorylated residueS9,S12 2457
Annotation ExtensionEvidenceResidueReference
IDA S9,S12 PMID:25720772
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for mra1 (SPAC18G6.07c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2272during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2332during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
2215during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1787during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
9531.97during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1909during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5612.66during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.1during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.93during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Disease Association
DescriptionQualifierReferenceCount
Bowen Conradi syndrome1
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in archaea242
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC18G6.07c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
modified byark1aurora-B kinase Ark1 Biochemical ActivityPMID:21712547
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC18G6.07c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
rescueszfs1CCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism Phenotypic SuppressionPMID:9133664
rescuesras1GTPase Ras1 Phenotypic SuppressionPMID:9133664
rescuesgap1GTPase activating protein Gap1 Phenotypic SuppressionPMID:9133664
External References
Database Identifier Description
NBRP SPAC18G6.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC18G6.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC18G6.07c BioGRID Interaction Datasets
Expression Viewer SPAC18G6.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC18G6.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC18G6.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC18G6.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC18G6.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC18G6.07c Transcriptome Viewer (Bähler Lab)
GEO SPAC18G6.07c GEO profiles
PInt SPAC18G6.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC18G6.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC18G6.07c Fission yeast phenotypic data & analysis
Cyclebase SPAC18G6.07c.1 Cell Cycle Data
SPD / RIKEN16/16G05Orfeome Localization Data
UniProtKB/SwissProtQ10107Ribosomal RNA small subunit methyltransferase mra1
ModBaseQ10107Database of comparative protein structure models
STRINGQ10107Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593671ribosome biogenesis protein Mra1
RefSeq mRNANM_001019103972h- ribosome biogenesis protein Mra1 (mra1), mRNA
European Nucleotide ArchiveBAA24497.1ENA Protein Mapping
European Nucleotide ArchiveCAA92394.1ENA Protein Mapping
UniParcUPI000012F4D7UniProt Archive

Literature for mra1

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016