lem2 (SPAC18G6.10)


Gene Standard Namelem2 Characterisation Statuspublished
Systematic IDSPAC18G6.10 Feature Typeprotein coding
Synonymsheh1 Name DescriptionLap-Emerin-Man domain protein,
Helix-Extension-Helix domain protein
ProductLEM domain protein Heh1/Lem2 Product Size688aa, 78.18 kDa
Genomic Location Chromosome I, 2234262-2236773 (2512nt); CDS:2234598-2236664 (2067nt)

Ensembl Gene Location
GO Biological Process
Term NameCount
meiotic attachment of telomere to nuclear envelope3
Annotation ExtensionEvidenceWith/FromReference
nuclear envelope organization12
Annotation ExtensionEvidenceWith/FromReference
nuclear inner membrane organization2
Annotation ExtensionEvidenceWith/FromReference
NOT centromere clustering at the nuclear envelope
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
integral component of nuclear inner membrane5
Annotation ExtensionEvidenceWith/FromReference
mitotic spindle pole body219
Annotation ExtensionEvidenceWith/FromReference
nuclear envelope129
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
normal growth on glucose carbon sourcelem2Δ282
sensitive to 5-fluorouracillem2Δ273
sensitive to hydroxyurealem2Δ580
viable vegetative cell populationlem2Δ3840

Cell Phenotype

Term NameGenotypesCount
abnormal meiosislem2Δ121
abnormal nuclear envelope morphology during vegetative growthlem2Δ17
abnormal telomere tethering at nuclear peripherylem2Δ4
cut during cellular response to hydroxyurealem2Δ44
decreased protein localization to nucleus during vegetative growth29
affects localization of nsp1lem2+
affects localization of nsp1lem2Δ
excess nuclear envelope presentlem2+2
normal mitotic sister chromatid segregationlem2Δ10
normal nuclear morphology during vegetative growthlem2Δ7
normal nuclear pore distributionlem2+6
normal protein localization to nucleus76
affecting nsp1lem2Δ
viable vegetative cell with normal cell morphologylem2Δ3100
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2234262..2236773
mRNA2234262..2236773
5' UTR2234262..2234597PMID:21511999
CDS2234598..2236664
3' UTR2236665..2236773PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF09402 Pfam IPR018996 Inner nuclear membrane protein MAN1 327 667 2
PF12949 Pfam IPR025856 HeH/LEM domain 11 44 2
TMhelix TMHMM 316 338 953
TMhelix TMHMM 548 567 953
PTHR13428:SF7 HMMPANTHER 2 675 2
PTHR13428 HMMPANTHER 2 675 2

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0001430LEM domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001430

Protein Properties

Ave. residue weight 113.63 Da
Charge 15.50
Codon Adaptation Index 0.37
Isoelectric point 8.34
Molecular weight 78.18 kDa
Number of residues 688
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for lem2 (SPAC18G6.10)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
682during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
597during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
682during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
934.46during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1245.3during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3.2during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.1during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC18G6.10 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds DNA-binding domain construct withima1inner nuclear membrane protein Ima1 Two-hybridPMID:21880100
binds DNA-binding domain construct withman1LEM domain protein Man1, Sad1 interacting factor Two-hybridPMID:26771498
binds DNA-binding domain construct withsad1spindle pole body protein Sad1 Two-hybridPMID:21880100
binds activation domain construct withsad1spindle pole body protein Sad1 Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC18G6.10 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withalp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
negative genetic interaction withssp2AMP-activated protein serine/threonine kinase alpha subunit Ssp2 Negative GeneticPMID:22681890
negative genetic interaction withksg1serine/threonine protein kinase Ksg1 Negative GeneticPMID:22681890
negative genetic interaction withset1histone lysine methyltransferase Set1 Negative GeneticPMID:22681890
negative genetic interaction withair1zinc knuckle TRAMP complex subunit Air1 Negative GeneticPMID:22681890
negative genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
negative genetic interaction withfkbp39FKBP-type peptidyl-prolyl cis-trans isomerase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmmb1mitochondrial microtubule binder Mmb1 Negative GeneticPMID:22681890
negative genetic interaction withamk2AMP-activated protein kinase beta subunit Amk2 Negative GeneticPMID:22681890
negative genetic interaction withrxt2histone deacetylase complex subunit Rxt2 Negative GeneticPMID:22681890
negative genetic interaction withago1argonaute Negative GeneticPMID:22681890
negative genetic interaction withelp3elongator complex subunit Elp3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
negative genetic interaction withcph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
negative genetic interaction withtas3RITS complex subunit 3 Negative GeneticPMID:22681890
negative genetic interaction withssu72phosphoric ester hydrolase Ssu72 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withprw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
enhances phenotype ofima1inner nuclear membrane protein Ima1 Phenotypic EnhancementPMID:21880100
synthetic growth defect withima1inner nuclear membrane protein Ima1 Synthetic Growth DefectPMID:21880100
positive genetic interaction withace2transcription factor Ace2 Positive GeneticPMID:22681890
phenotype enhanced byman1LEM domain protein Man1, Sad1 interacting factor Phenotypic EnhancementPMID:21880100
External References
Database Identifier Description
NBRP SPAC18G6.10 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC18G6.10 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC18G6.10 BioGRID Interaction Datasets
Expression Viewer SPAC18G6.10 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC18G6.10 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC18G6.10 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC18G6.10 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC18G6.10 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC18G6.10 Transcriptome Viewer (Bähler Lab)
GEO SPAC18G6.10 GEO profiles
PInt SPAC18G6.10 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC18G6.10 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC18G6.10 Fission yeast phenotypic data & analysis
Cyclebase SPAC18G6.10.1 Cell Cycle Data
SPD / RIKEN46/46D11Orfeome Localization Data
UniProtKB/SwissProtQ10109Lap-Emerin-Man domain protein 2
ModBaseQ10109Database of comparative protein structure models
STRINGQ10109Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593673LEM domain protein Heh1/Lem2
RefSeq mRNANM_001019105972h- LEM domain protein Heh1/Lem2 (lem2), mRNA

Literature for lem2

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016