mal3 (SPAC18G6.15)


Gene Standard Namemal3 Characterisation Statuspublished
Systematic IDSPAC18G6.15 Feature Typeprotein coding
Synonyms Name Description
ProductEB1 family Mal3 Product Size308aa, 35.09 kDa
Genomic Location Chromosome I, 2243558-2245220 (1663nt); CDS:2243636-2244622 (987nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0001671ATPase activator activityIDAPMID:156653793
GO:0008017microtubule bindingIDAPMID:1566537920
GO:0051010microtubule plus-end bindingIDAPMID:150620955
GO:0005515protein bindingIPItea2PMID:12894167887
IPImoe1PMID:11102508
IPItip1PMID:15062095
IPIspc7PMID:15371542
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0051315attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregationIGIbub1PMID:1639410524
GO:0034613cellular protein localization556
localizes tip1IMPPMID:15062095
localizes tea2IMPPMID:12894167
GO:0061246establishment or maintenance of bipolar cell polarity regulating cell shapeNASGO_REF:00000015
GO:0033566gamma-tubulin complex localizationIMPPMID:111025086
GO:0007019microtubule depolymerizationISSUniProtKB:P40013GO_REF:00000012
GO:0046785microtubule polymerizationIMPPMID:93482882
GO:0007018microtubule-based movement23
requiredIMPPMID:12894167
GO:0000745nuclear migration involved in conjugation with mutual genetic exchangeIMPPMID:237551761
GO:0032781positive regulation of ATPase activityIDAPMID:156653793
GO:0035372protein localization to microtubule1
localizes klp2IDAPMID:24039245
GO:0008360regulation of cell shapeIMPPMID:934828862
GO:0010570regulation of filamentous growthIMPPMID:1954231213
GO:0007088regulation of mitosisIGIbub3PMID:1639410581
IGImad3PMID:16394105
IGIbub1PMID:16394105
GO:0090307spindle assembly involved in mitosisIMPPMID:1110250817
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000235astral microtubuleIDAPMID:163941057
GO:0051285cell cortex of cell tipIDAPMID:1506209538
GO:0005881cytoplasmic microtubuleIDAPMID:1289416710
IDAPMID:16394105
GO:0005829cytosolIDAPMID:168233722317
GO:0005828kinetochore microtubuleIDAPMID:153715421
GO:0015630microtubule cytoskeletonIDAPMID:11102508288
IDAPMID:9348288
IDAPMID:16823372
GO:0005634nucleusIDAPMID:168233722730
GO:0051233spindle midzoneIDAPMID:1639410511
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000240abnormal filament morphologymal3ΔNullPMID:195423125
FYPO:0001098sensitive to 4-nitroquinoline N-oxideCell growth assaymal3ΔNullPECO:0000137, PECO:0000005PMID:19264558159
expressivity FYPO_EXT:0000003
FYPO:0000091sensitive to thiabendazolecompetitive growth assay evidencemal3ΔNullPMID:2053713292
FYPO:0002060viable vegetative cell populationMicroscopymal3ΔNullPECO:0000005, PECO:0000137PMID:236978063755
no_namePMID:16394105
Microscopymal3ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003065abnormal microtubule cytoskeleton morphology during matingMicroscopymal3ΔNullPMID:237551761
FYPO:0003062abnormal nuclear migration during matingMicroscopymal3ΔNullPMID:237551761
FYPO:0001894abnormal sporulation resulting in formation of ascus with more or fewer than four sporesMicroscopymal3ΔNullPECO:0000015PMID:2375517616
penetrance FYPO_EXT:0000002
FYPO:0000512decreased nuclear fusion during matingMicroscopymal3ΔNullPMID:237551761
FYPO:0000581decreased spore germinationMicroscopymal3ΔNullPECO:0000015PMID:2375517612
FYPO:0002151inviable sporeMicroscopymal3ΔNullPMID:23755176447
penetrance FYPO_EXT:0000001
FYPO:0002487normal actin cable morphologyMicroscopymal3ΔNullPMID:230517343
FYPO:0001147normal mating efficiencyMicroscopymal3ΔNullPECO:0000015PMID:2375517612
FYPO:0003061twin haploid meiosisMicroscopymal3ΔNullPMID:237551761
FYPO:0002112viable curved vegetative cellMicroscopymal3ΔNullPECO:0000137, PECO:0000005PMID:2369780657
expressivity FYPO_EXT:0000003
penetrance FYPO_EXT:0000003
Microscopymal3ΔNullPMID:23051734
Target Of
RelationshipGeneProductReference
localised by klp2 kinesin-like protein Klp2 PMID:24039245
localized by tea2 kinesin-like protein Tea2 PMID:15665379
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
122435582243677
222437382245220

UTRs

Region Start End Reference
five_prime_UTR22435582243635PMID:21511999
three_prime_UTR22446232245220PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00307 Pfam IPR001715 Calponin homology domain 5 98 6
PF03271 Pfam IPR004953 EB1, C-terminal 197 240 1
PS51230 Prosite Profiles IPR004953 EB1, C-terminal 173 247 1
PTHR10623:SF6 HMMPANTHER 1 307 1
PTHR10623 HMMPANTHER IPR027328 Microtubule-associated protein RP/EB 1 307 1
G3DSA:1.10.418.10 Gene3D IPR001715 3 118 6
SSF140612 SuperFamily IPR004953 180 246 1
SSF47576 SuperFamily IPR001715 3 117 6
Coil ncoils Rabaptin coiled-coil domain 167 195 975
Low complexity (SEG) seg 144 171
Low complexity (SEG) seg 253 264

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0001435calponin homology (CH) domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001435

Protein Properties

Ave. residue weight 113.94 Da
Charge -10.50
Isoelectric point 4.60
Molecular weight 35.09 kDa
Number of residues 308
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
5234.28during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2171.13during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
7during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.95during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReferenceCount
functionally complemented by human MAPRE11
Miscellaneous Functional Group
DescriptionQualifierReferenceCount
plus end tracking protein3
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
hip1hira protein, histone chaperone Hip1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
mph1dual specificity protein kinase Mph1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
Synthetic Growth DefectPMID:16179942
Negative GeneticPMID:22681890
rik1silencing protein Rik1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
spc34DASH complex subunit Spc34 Negative GeneticPMID:18818364
eme1Holliday junction resolvase subunit Eme1 Negative GeneticPMID:18818364
elp3elongator complex, histone acetyltransferase subunit Elp3 (predicted) Negative GeneticPMID:18818364
peg1CLASP family microtubule-associated protein Synthetic RescuePMID:16951255
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
ers1RNA-silencing factor Ers1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
ash2Ash2-trithorax family protein Negative GeneticPMID:18818364
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:18818364
dad2DASH complex subunit Dad2 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
tma23ribosome biogenesis protein Tma23 (predicted) Negative GeneticPMID:18818364
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
amo1nuclear rim protein Amo1 Negative GeneticPMID:18818364
tas3RITS complex subunit 3 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
mei4meiotic forkhead transcription factor Mei4 Negative GeneticPMID:18818364
sts5RNB-like protein Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
usp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
pac2cAMP-independent regulatory protein Pac2 Negative GeneticPMID:22681890
swi6HP1 family chromodomain protein Swi6 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
ran1serine/threonine protein kinase Ran1 Phenotypic EnhancementPMID:23755176
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
SPAC1805.16cpurine nucleoside phosphorylase (predicted) Negative GeneticPMID:22681890
ryh1GTPase Ryh1 Negative GeneticPMID:18818364
dhc1dynein heavy chain Dhc1 Phenotypic EnhancementPMID:23755176
sum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:18818364
ams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
SPBC582.10cATP-dependent DNA helicase Rhp16b (predicted) Positive GeneticPMID:22681890
duo1DASH complex subunit Duo1 Negative GeneticPMID:22681890
caf1CCR4-Not complex CAF1 family ribonuclease subunit Caf1 Negative GeneticPMID:18818364
ago1argonaute Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
elp4elongator complex subunit Elp4 (predicted) Negative GeneticPMID:18818364
mad2spindle checkpoint protein Mad2 Phenotypic SuppressionPMID:20498706
ssm4dynactin microtubule-binding subunit, p150-Glued Ssm4 Phenotypic EnhancementPMID:23755176
efc25Ras1 guanyl-nucleotide exchange factor Efc25 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
rxt2histone deacetylase complex subunit Rxt2 Phenotypic EnhancementPMID:19547744
myo52myosin type V Phenotypic EnhancementPMID:23051734
mre11Mre11 nuclease Negative GeneticPMID:18818364
raf2Rik1-associated factor Raf2 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
moe1translation initiation factor eIF3d Moe1 Synthetic Growth DefectPMID:11102508
myo51myosin type V Phenotypic EnhancementPMID:23051734
pom1DYRK family protein kinase Pom1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
dad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
asp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Phenotypic EnhancementPMID:20624911
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
rsc1RSC complex subunit Rsc1 Negative GeneticPMID:18818364
SPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
sgf29SAGA complex subunit Sgf29 Negative GeneticPMID:18818364
int6eIF3e subunit Int6 Negative GeneticPMID:22681890
par1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:18818364
SPAC1805.14sequence orphan Negative GeneticPMID:22681890
hrp3ATP-dependent DNA helicase Hrp3 Negative GeneticPMID:22681890
spi1Ran GTPase Spi1 Synthetic Growth DefectPMID:11086011
Phenotypic Enhancement
sid2NDR kinase Sid2 Phenotypic EnhancementPMID:23087209
for3formin For3 Synthetic Growth DefectPMID:18931302
alp14TOG ortholog Alp14 Negative GeneticPMID:18818364
Synthetic LethalityPMID:15371542
rdp1RNA-directed RNA polymerase Rdp1 Negative GeneticPMID:18818364
ase1antiparallel microtubule cross-linking factor Ase1 Synthetic Growth DefectPMID:18931302
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
set1histone lysine methyltransferase Set1 Negative GeneticPMID:18818364
cay1cactin, spliceosome complex subunit (predicted) Negative GeneticPMID:18818364
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
SPAC23A1.07ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:18818364
rpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
rim20BRO1 domain protein Rim20 (predicted) Synthetic Growth DefectPMID:18931302
clp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:18931302
mad3mitotic spindle checkpoint protein Mad3 Synthetic Growth DefectPMID:16179942
mag1DNA-3-methyladenine glycosylase Mag1 Negative GeneticPMID:22681890
apq12nuclear membrane organization protein Apq12 (predicted) Negative GeneticPMID:22681890
bub1serine/threonine protein kinase Bub1 Synthetic Growth DefectPMID:16179942
cdc16two-component GAP Cdc16 Phenotypic SuppressionPMID:20498706
spc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
nrm1MBF complex corepressor nrm1 Negative GeneticPMID:22681890
ppk30Ark1/Prk1 family protein kinase Ppk30 Positive GeneticPMID:18818364
fin1serine/threonine protein kinase, NIMA related Fin1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
rsc4RSC complex subunit Rsc4 Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:18818364
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
swd3WD repeat protein Swd3 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
spf1Set1C PHD Finger protein Spf1 Negative GeneticPMID:18818364
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
pab2poly(A) binding protein Pab2 Positive GeneticPMID:22681890
SPAC15E1.10PI31 proteasome inhibitor (predicted) Positive GeneticPMID:22681890
pub1HECT-type ubiquitin-protein ligase E3 Pub1 Negative GeneticPMID:18818364
pho24-nitrophenylphosphatase Positive GeneticPMID:22681890
tip1CLIP170 family protein Tip1 Phenotypic SuppressionPMID:23613905
swd2Set1C complex subunit Swd2.1 Negative GeneticPMID:18818364
rrp1ATP-dependent DNA helicase (predicted) Positive GeneticPMID:22681890
cbp1CENP-B homolog Negative GeneticPMID:18818364
spn1septin Spn1 Negative GeneticPMID:18818364
mad1mitotic spindle checkpoint protein Mad1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
lsk1P-TEFb-associated cyclin-dependent protein kinase Lsk1 Negative GeneticPMID:18818364
mid1medial ring protein Mid1 Negative GeneticPMID:22681890
cid12poly(A) polymerase Cid12 Negative GeneticPMID:18818364
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
mis4adherin, cohesin loading factor Mis4 Negative GeneticPMID:23050226
SPBC13E7.03cRNA hairpin binding protein (predicted) Negative GeneticPMID:22681890
bub3mitotic spindle checkpoint protein Bub3 Synthetic Growth DefectPMID:16179942
tea2kinesin-like protein Tea2 Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
Phenotypic EnhancementPMID:19547744
ubp2ubiquitin C-terminal hydrolase Ubp2 Negative GeneticPMID:22681890
mto1MT organizer Mto1 Negative GeneticPMID:22681890
zds1zds family protein phosphatase type A regulator Zds1 (predicted) Negative GeneticPMID:18818364
atb2tubulin alpha 2 Synthetic Growth DefectPMID:16394105
Dosage Rescue
Positive GeneticPMID:22681890
dad3DASH complex subunit Dad3 Negative GeneticPMID:22681890
sdc1Dpy-30 domain protein Sdc1 Negative GeneticPMID:18818364
arp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:18818364
SPBC15C4.06cubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
res2MBF transcription factor complex subunit Res2 Negative GeneticPMID:18818364
tos4FHA domain protein Tos4 (predicted) Negative GeneticPMID:22681890
raf1Rik1-associated factor Raf1 Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
dcr1dicer Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
Negative GeneticPMID:22681890
spc7kinetochore protein Spc7 Dosage RescuePMID:15371542
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
peg1CLASP family microtubule-associated protein Affinity Capture-WesternPMID:16951255
Two-hybrid
nda2tubulin alpha 1 Co-crystal StructurePMID:22500803
Reconstituted ComplexPMID:18794845
nda3tubulin beta Nda3 Co-crystal StructurePMID:22500803
moe1translation initiation factor eIF3d Moe1 Two-hybridPMID:11102508
Reconstituted Complex
cds1replication checkpoint kinase Cds1 Affinity Capture-WesternPMID:21336311
tea2kinesin-like protein Tea2 Co-fractionationPMID:15665379
Affinity Capture-WesternPMID:15177031
spc7kinetochore protein Spc7 Affinity Capture-WesternPMID:15371542
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
atb2tubulin alpha 2 Reconstituted ComplexPMID:17190604
tip1CLIP170 family protein Tip1 Two-hybridPMID:16951255
Affinity Capture-WesternPMID:15177031
Reconstituted Complex
Reconstituted ComplexPMID:15062095
Affinity Capture-Western
klp2kinesin-like protein Klp2 Reconstituted ComplexPMID:23087209
External References
Database Identifier Description
NBRP SPAC18G6.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC18G6.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC18G6.15 BioGRID Interaction Datasets
Expression Viewer SPAC18G6.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC18G6.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC18G6.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC18G6.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC18G6.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC18G6.15 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC18G6.15 Cell Cycle Data
GEO SPAC18G6.15 GEO profiles
PInt SPAC18G6.15 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542123EB1 family Mal3
EntrezGene2542123EB1 family Mal3
SPD / RIKEN14/14C11Orfeome Localization Data
UniProtKB/SwissProtQ10113Microtubule integrity protein mal3
ModBaseQ10113Database of comparative protein structure models
StringQ10113Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593678EB1 family Mal3
RefSeq mRNANM_001019110972h- EB1 family Mal3 (mal3), mRNA
European Nucleotide ArchiveCAA70707ENA Protein Mapping
European Nucleotide ArchiveCAA92392ENA Protein Mapping
UniParcUPI000012EB3EUniProt Archive

Literature for mal3

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014