mal3 (SPAC18G6.15)

Gene Standard Namemal3 ChromosomeI
Systematic IDSPAC18G6.15 Gene Start2243558
Synonyms Gene End2245220
ProductEB1 family Mal3 Gene Length1663
Feature Typeprotein coding CDS Start2243636
Name Description CDS End2244622
Characterisation Statuspublished CDS Length987

Ensembl Gene Location
  1. rrn3
  2. SPAC18G6.12c
  3. SPNCRNA.806
  4. SPAC18G6.13
  5. rps7
  6. mal3
  7. pof14
  8. SPNCRNA.807
  9. byr3
  10. SPNCRNA.189
  11. SPATRNAGLY.02
  12. trm9
  13. btb3
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000091sensitive to thiabendazolecompetitive growth assay evidencemal3delta (deletion)deletionPMID:20537132
FYPO:0000204abnormal mRNA export from nucleusmal3delta (deletion)deletionPMID:19542312
FYPO:0000269sensitive to microtubule depolymerizing drugscompetitive growth assay evidencemal3delta (deletion)deletionPMID:20537132
FYPO:0002060viable vegetative cell populationno_name (unrecorded)unrecordedPMID:16394105
Microscopymal3delta (deletion)deletionPMID:20473289
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0001671ATPase activator activityIDAPMID:15665379
GO:0005515protein bindingIPIspc7PMID:15371542
IPItip1PMID:15062095
IPItea2PMID:12894167
IPImoe1PMID:11102508
GO:0008017microtubule bindingIDAPMID:15665379
GO:0051010microtubule plus-end bindingIDAPMID:15062095
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0007018microtubule-based movement
requiredIMPPMID:12894167
GO:0007019microtubule depolymerizationISSUniProtKB:P40013GO_REF:0000001
GO:0007088regulation of mitosisIGImad3PMID:16394105
IGIbub3PMID:16394105
IGIbub1PMID:16394105
GO:0008360regulation of cell shapeIMPPMID:9348288
GO:0008608attachment of spindle microtubules to kinetochoreIGIbub1PMID:16394105
GO:0010570regulation of filamentous growthIMPPMID:19542312
GO:0033566gamma-tubulin complex localizationIMPPMID:11102508
GO:0034613cellular protein localization
has_input tea2IMPPMID:12894167
has_input tip1IMPPMID:15062095
GO:0046785microtubule polymerizationIMPPMID:9348288
GO:0061246establishment or maintenance of bipolar cell polarity regulating cell shapeNASGO_REF:0000001
GO:0090307spindle assembly involved in mitosisIMPPMID:11102508
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0000235astral microtubuleIDAPMID:16394105
GO:0005634nucleusIDAPMID:16823372
GO:0005828kinetochore microtubuleIDAPMID:15371542
GO:0005829cytosolIDAPMID:16823372
GO:0005881cytoplasmic microtubuleIDAPMID:12894167
IDAPMID:16394105
GO:0015630microtubule cytoskeletonIDAPMID:9348288
IDAPMID:16823372
IDAPMID:11102508
GO:0051233spindle midzoneIDAPMID:16394105
GO:0051285cell cortex of cell tipIDAPMID:15062095
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
122435582243677
222437382245220

References

Region Start End Reference
three_prime_UTR22446232245220PMID:21511999
three_prime_UTR22446232245220PMID:21511999
five_prime_UTR22435582243635PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF03271 IPR004953 Pfam EB1, C-terminal 197 240 1
PF00307 IPR001715 Pfam Calponin homology domain 6 97 6
PS51230 IPR004953 Prosite Profiles EB1, C-terminal 173 247 1
G3DSA:1.10.418.10 IPR001715 Gene3D 3 118 6
SSF140612 SuperFamily 180 246 1
SSF47576 IPR001715 SuperFamily 3 117 6
Coil ncoils Rabaptin coiled-coil domain 167 195 974
PTHR10623:SF6 hmmpanther 4 307 1
PTHR10623 hmmpanther Microtubule-associated protein RP/EB 4 307 1

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0001435calponin homology (CH) domainTemporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0001435

Protein Properties

Ave. residue weight 113.94 Da
Charge -10.50
Isoelectric point 4.60
Molecular weight 35.09 kDa
Number of residues 308
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide2171.13PMID:23101633
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide5234.28PMID:23101633
RNA levelexperimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide0.95PMID:23101633
experimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide7PMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by human MAPRE1
Miscellaneous Functional Group
DescriptionQualifierReference
plus end tracking protein
Species Distribution
DescriptionQualifierReference
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
SPAC15E1.10PI31 proteasome inhibitor (predicted) Positive GeneticPMID:22681890
clp1Cdc14-related protein phosphatase Clp1/Flp1 Synthetic Growth DefectPMID:18931302
alp14TOG ortholog Alp14 Synthetic LethalityPMID:15371542
Negative GeneticPMID:18818364
tea2kinesin-like protein Tea2 Positive GeneticPMID:22681890
duo1DASH complex subunit Duo1 Negative GeneticPMID:22681890
sdc1Dpy-30 domain protein Sdc1 Negative GeneticPMID:18818364
dad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
prw1Clr6 histone deacetylase complex subunit Prw1 Negative GeneticPMID:22681890
sid2NDR kinase Sid2 Phenotypic EnhancementPMID:23087209
rrp1ATP-dependent DNA helicase (predicted) Positive GeneticPMID:22681890
prp4serine/threonine protein kinase Prp4 Negative GeneticPMID:22681890
mid1medial ring protein Mid1 Negative GeneticPMID:22681890
mto1MT organizer Mto1 Negative GeneticPMID:22681890
atb2tubulin alpha 2 Synthetic Growth DefectPMID:16394105
Dosage Rescue
Positive GeneticPMID:22681890
ash2Ash2-trithorax family protein Negative GeneticPMID:18818364
fin1serine/threonine protein kinase, NIMA related Fin1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
set1histone lysine methyltransferase Set1 Negative GeneticPMID:18818364
myo51myosin type V Phenotypic EnhancementPMID:23051734
hip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
mph1dual specificity protein kinase Mph1 Synthetic Growth DefectPMID:16179942
Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
usp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
ago1argonaute Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
spc34DASH complex subunit Spc34 Negative GeneticPMID:18818364
apq12nuclear membrane organization protein Apq12 (predicted) Negative GeneticPMID:22681890
mei4meiotic forkhead transcription factor Mei4 Negative GeneticPMID:18818364
ase1antiparallel microtubule cross-linking factor Ase1 Synthetic Growth DefectPMID:18931302
sts5RNB-like protein Negative GeneticPMID:22681890
SPBC15C4.06cubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
spf1Set1C PHD Finger protein Spf1 Negative GeneticPMID:18818364
dad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:18818364
caf1CCR4-Not complex CAF1 family ribonuclease subunit Caf1 Negative GeneticPMID:18818364
SPAC1805.16cpurine nucleoside phosphorylase (predicted) Negative GeneticPMID:22681890
ngg1SAGA complex subunit Ngg1 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
spn1septin Spn1 Negative GeneticPMID:18818364
myo52myosin type V Phenotypic EnhancementPMID:23051734
ers1RNA-silencing factor Ers1 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
swd2Set1C complex subunit Swd2.1 Negative GeneticPMID:18818364
spc7kinetochore protein Spc7 Dosage RescuePMID:15371542
zds1zds family protein phosphatase type A regulator Zds1 (predicted) Negative GeneticPMID:18818364
mag1DNA-3-methyladenine glycosylase Mag1 Negative GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
rxt2histone deacetylase complex subunit Rxt2 Phenotypic EnhancementPMID:19547744
par1protein phosphatase regulatory subunit Par1 Negative GeneticPMID:18818364
mad2spindle checkpoint protein Mad2 Phenotypic SuppressionPMID:20498706
nrm1negative regulator of MBF Negative GeneticPMID:22681890
cdc16two-component GAP Cdc16 Phenotypic SuppressionPMID:20498706
SPAP14E8.02FHA domain protein Tos4 (predicted) Negative GeneticPMID:22681890
res2MBF transcription factor complex subunit Res2 Negative GeneticPMID:18818364
rim20BRO1 domain protein Rim20 (predicted) Synthetic Growth DefectPMID:18931302
spi1Ran GTPase Spi1 Synthetic Growth DefectPMID:11086011
Phenotypic Enhancement
ppk30Ark1/Prk1 family protein kinase Ppk30 Positive GeneticPMID:18818364
swd3WD repeat protein Swd3 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
dad3DASH complex subunit Dad3 Negative GeneticPMID:22681890
rsc1RSC complex subunit Rsc1 Negative GeneticPMID:18818364
asp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Phenotypic EnhancementPMID:20624911
rpa12DNA-directed RNA polymerase complex I subunit Rpa12 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
int6eIF3e subunit Int6 Negative GeneticPMID:22681890
pom1DYRK family protein kinase Pom1 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
for3formin For3 Synthetic Growth DefectPMID:18931302
tas3RITS complex subunit 3 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
raf2Rik1-associated factor Raf2 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Phenotypic EnhancementPMID:19547744
Negative GeneticPMID:22681890
peg1CLASP family microtubule-associated protein Synthetic RescuePMID:16951255
elp3elongator complex, histone acetyltransferase subunit Elp3 (predicted) Negative GeneticPMID:18818364
pho24-nitrophenylphosphatase Positive GeneticPMID:22681890
hrp3ATP-dependent DNA helicase Hrp3 Negative GeneticPMID:22681890
ypa2protein phosphatase type 2A regulator, PTPA family Ypa2 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
hus1checkpoint clamp complex protein Hus1 Positive GeneticPMID:22681890
efc25Ras1 guanyl-nucleotide exchange factor Efc25 Negative GeneticPMID:22681890
Synthetic Growth DefectPMID:18931302
ams2cell cycle regulated GATA-type transcription factor Ams2 Negative GeneticPMID:22681890
tma23ribosome biogenesis protein Tma23 (predicted) Negative GeneticPMID:18818364
bub1serine/threonine protein kinase Bub1 Synthetic Growth DefectPMID:16179942
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
SPAC23A1.07ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:18818364
pac2cAMP-independent regulatory protein Pac2 Negative GeneticPMID:22681890
hrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
amo1nuclear rim protein Amo1 Negative GeneticPMID:18818364
cid12poly(A) polymerase Cid12 Negative GeneticPMID:18818364
rdp1RNA-directed RNA polymerase Rdp1 Negative GeneticPMID:18818364
SPBC13E7.03cRNA hairpin binding protein (predicted) Negative GeneticPMID:22681890
raf1Rik1-associated factor Raf1 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
mre11Mre11 nuclease Negative GeneticPMID:18818364
sum2G2/M transition checkpoint protein Sum2 Negative GeneticPMID:18818364
gcn5SAGA complex histone acetyltransferase catalytic subunit Gcn5 Negative GeneticPMID:18818364
mis4adherin, cohesin loading factor Mis4 Negative GeneticPMID:23050226
SPAC1805.14sequence orphan Negative GeneticPMID:22681890
sgf29SAGA complex subunit Sgf29 Negative GeneticPMID:18818364
ryh1GTPase Ryh1 Negative GeneticPMID:18818364
bub3mitotic spindle checkpoint protein Bub3 Synthetic Growth DefectPMID:16179942
moe1translation initiation factor eIF3d Moe1 Synthetic Growth DefectPMID:11102508
spc19DASH complex subunit Spc19 Negative GeneticPMID:22681890
lsk1P-TEFb-associated cyclin-dependent protein kinase Lsk1 Negative GeneticPMID:18818364
elp4elongator complex subunit Elp4 (predicted) Negative GeneticPMID:18818364
mad3mitotic spindle checkpoint protein Mad3 Synthetic Growth DefectPMID:16179942
rsc4RSC complex subunit Rsc4 Negative GeneticPMID:22681890
eme1Holliday junction resolvase subunit Eme1 Negative GeneticPMID:18818364
arp8actin-like protein, Ino80 complex subunit Arp8 Negative GeneticPMID:18818364
pub1HECT-type ubiquitin-protein ligase E3 Pub1 Negative GeneticPMID:18818364
cbp1CENP-B homolog Negative GeneticPMID:18818364
rik1silencing protein Rik1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
dcr1dicer Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
mad1mitotic spindle checkpoint protein Mad1 Synthetic Growth DefectPMID:18931302
Negative GeneticPMID:18818364
pab2poly(A) binding protein Pab2 Positive GeneticPMID:22681890
res1MBF transcription factor complex subunit Res1 Negative GeneticPMID:22681890
SPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
ubp2ubiquitin C-terminal hydrolase Ubp2 Negative GeneticPMID:22681890
cay1cactin, spliceosome complex subunit (predicted) Negative GeneticPMID:18818364
swi6HP1 family chromodomain protein Swi6 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
Negative GeneticPMID:18818364
SPBC582.10cATP-dependent DNA helicase Rhp16b (predicted) Positive GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
spc7kinetochore protein Spc7 Affinity Capture-WesternPMID:15371542
alp14TOG ortholog Alp14 Co-localizationPMID:22696680
peg1CLASP family microtubule-associated protein Two-hybridPMID:16951255
Affinity Capture-Western
tea2kinesin-like protein Tea2 Co-localizationPMID:12894167
Co-fractionationPMID:15665379
Affinity Capture-WesternPMID:15177031
klp2kinesin-like protein Klp2 Reconstituted ComplexPMID:23087209
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
nda3tubulin beta Nda3 Co-crystal StructurePMID:22500803
moe1translation initiation factor eIF3d Moe1 Two-hybridPMID:11102508
Reconstituted Complex
nda2tubulin alpha 1 Reconstituted ComplexPMID:18794845
Co-crystal StructurePMID:22500803
atb2tubulin alpha 2 Reconstituted ComplexPMID:17190604
cds1replication checkpoint kinase Cds1 Affinity Capture-WesternPMID:21336311
tip1CLIP170 family protein Tip1 Two-hybridPMID:16951255
Reconstituted ComplexPMID:15062095
Co-localization
Affinity Capture-Western
Reconstituted ComplexPMID:15177031
Affinity Capture-Western
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External References
Database Identifier Description
NBRP SPAC18G6.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC18G6.15 Retrieval of eukaryotic orthologs
BioGrid SPAC18G6.15 BioGRID Interaction Datasets
Bähler Lab SPAC18G6.15 Cell Cycle Expression Profile
Bähler Lab SPAC18G6.15 Meiosis/Sporulation Expression Profies
Bähler Lab SPAC18G6.15 Pheromone response/mating expression profiles
Bähler Lab SPAC18G6.15 Environmental stress expression profiles
Bähler Lab SPAC18G6.15 Bähler Lab Transcriptome Viewer
Cyclebase SPAC18G6.15 Cell Cycle Data
PInt SPAC18G6.15 Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC18G6.15 Entrez Gene
WikiGene2542123EB1 family Mal3
Uniprot_genenameMAL3
EntrezGene2542123EB1 family Mal3
SPD / RIKEN14/14C11Orfeome Localization Data
UniProtKB/SwissProtQ10113Microtubule integrity protein mal3
ModBaseQ10113Database of comparative protein structure models
Pfam Protein DomainsQ10113Pfam Domain Arrangement
RefSeq PeptideNP_593678EB1 family Mal3
RefSeq mRNANM_001019110972h- EB1 family Mal3 (mal3), mRNA
European Nucleotide ArchiveCAA70707ENA Protein Mapping
European Nucleotide ArchiveCAA92392ENA Protein Mapping
UniParcUPI000012EB3EUniProt Archive

Literature for mal3
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013