trp2 (SPAC19A8.15)


Gene Standard Nametrp2 Characterisation Statusbiological_role_inferred
Systematic IDSPAC19A8.15 Feature Typeprotein coding
Synonyms Name Description
Producttryptophan synthase (predicted) Product Size697aa, 75.44 kDa
Genomic Location Chromosome I, 2459817-2457495 (2323nt); CDS:2459751-2457658 (2094nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0004834tryptophan synthase activityISOSGD:S000002994GO_REF:00000241
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000162tryptophan biosynthetic processISOSGD:S000002994GO_REF:00000245
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmISOSGD:S000002994GO_REF:00000244201
GO:0005829cytosolIDAPMID:168233722316
GO:0005634nucleusIDAPMID:168233722740
ISOSGD:S000002994GO_REF:0000024
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopytrp2ΔNullPMID:204732891315
Microscopytrp2ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002420inviable after spore germination, single cell division, abnormal cell shapeMicroscopytrp2ΔNullPECO:0000005, PECO:0000137PMID:2369780647
FYPO:0002151inviable sporeMicroscopytrp2ΔNullPECO:0000005, PECO:0000137PMID:23697806457
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
124598172457495

UTRs

Region Coordinates Reference
five_prime_UTR2459817..2459752PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00291 Pfam IPR001926 Tryptophan synthase beta subunit-like PLP-dependent enzymes superfamily 360 671 7
PF00290 Pfam IPR002028 Tryptophan synthase, alpha chain 9 259 1
PS00167 Prosite Patterns IPR018204 Tryptophan synthase, alpha chain, active site 49 62 1
PS00168 Prosite Patterns IPR006653 Tryptophan synthase, beta chain, conserved site 374 388 1
PTHR10314 HMMPANTHER 38 697 7
PTHR10314:SF3 HMMPANTHER IPR006654 Tryptophan synthase, beta chain 38 697 1
3.40.50.1100 Gene3D 471 684 7
3.40.50.1100 Gene3D 392 470 7
3.20.20.70 Gene3D IPR013785 Aldolase-type TIM barrel 3 265 35
SSF53686 SuperFamily IPR001926 Tryptophan synthase beta subunit-like PLP-dependent enzymes superfamily 301 682 7
SSF51366 SuperFamily IPR011060 Ribulose-phosphate binding barrel 4 257 7
TIGR00262 tigrfam IPR002028 Tryptophan synthase, alpha chain 11 261 1
TIGR00263 tigrfam IPR006654 Tryptophan synthase, beta chain 302 683 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.23 Da
Charge 1.00
Isoelectric point 6.62
Molecular weight 75.44 kDa
Number of residues 697
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residueNASPMID:195477441915
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
96064during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
91095during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
90298during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
91268during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
22459.12during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
77683.15during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
95987during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
15during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
paa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
far8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
External References
Database Identifier Description
NBRP SPAC19A8.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC19A8.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC19A8.15 BioGRID Interaction Datasets
Expression Viewer SPAC19A8.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC19A8.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC19A8.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC19A8.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC19A8.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC19A8.15 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC19A8.15 Cell Cycle Data
GEO SPAC19A8.15 GEO profiles
PInt SPAC19A8.15 Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz4.2.1.20Integrated relational Enzyme database
Rhea4.2.1.20Annotated reactions database
WikiGene2542240tryptophan synthase (predicted)
EntrezGene2542240tryptophan synthase (predicted)
SPD / RIKEN27/27H01Orfeome Localization Data
UniProtKB/SwissProtO13831Tryptophan synthase
ModBaseO13831Database of comparative protein structure models
STRINGO13831Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593777tryptophan synthase (predicted)
RefSeq mRNANM_001019206972h- tryptophan synthase (predicted) (trp2), mRNA
European Nucleotide ArchiveD89113ENA EMBL mapping
European Nucleotide ArchiveBAA13775ENA Protein Mapping
European Nucleotide ArchiveCAB11651ENA Protein Mapping
European Nucleotide ArchiveCAB11651.1ENA Protein Mapping
UniParcUPI000013764BUniProt Archive

Literature for trp2

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014