trp2 (SPAC19A8.15)


Gene Standard Nametrp2 Characterisation Statusbiological role inferred
Systematic IDSPAC19A8.15 Feature Typeprotein coding
Synonyms Name Description
Producttryptophan synthase (predicted) Product Size697aa, 75.44 kDa
Genomic Location Chromosome I, 2459817-2457495 (2323nt); CDS:2459751-2457658 (2094nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
tryptophan synthase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
tryptophan biosynthetic process5
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
nucleus2692
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationtrp2Δ1453

Cell Phenotype

Term NameGenotypesCount
inviable after spore germination, single cell division, abnormal cell shapetrp2Δ49
inviable sporetrp2Δ476
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons2459817..2457495
5' UTR2459817..2459752PMID:21511999
3' UTR2457657..2457495AU011243
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00291 Pfam IPR001926 Tryptophan synthase beta subunit-like PLP-dependent enzyme 347 671 7
PF00290 Pfam IPR002028 Tryptophan synthase, alpha chain 9 260 1
PS00168 Prosite Patterns IPR006653 Tryptophan synthase, beta chain, conserved site 374 388 1
PS00167 Prosite Patterns IPR018204 Tryptophan synthase, alpha chain, active site 49 62 1
PTHR10314 HMMPANTHER 301 697 7
PTHR10314:SF3 HMMPANTHER IPR006654 Tryptophan synthase, beta chain 301 697 1
3.40.50.1100 Gene3D Threonine dehydratase, catabolic 392 470 7
3.40.50.1100 Gene3D Threonine dehydratase, catabolic 471 684 7
3.20.20.70 Gene3D IPR013785 Aldolase-type TIM barrel 3 265 35
SSF53686 SuperFamily IPR001926 Tryptophan synthase beta subunit-like PLP-dependent enzyme 301 682 7
SSF51366 SuperFamily IPR011060 Ribulose-phosphate binding barrel 4 257 7
MF_00131 HAMAP IPR002028 Tryptophan synthase, alpha chain 15 266 1
MF_00133 HAMAP IPR023026 Tryptophan synthase beta chain/beta chain-like 296 684 1
TIGR00263 TIGRFAM IPR006654 Tryptophan synthase, beta chain 302 683 1
TIGR00262 TIGRFAM IPR002028 Tryptophan synthase, alpha chain 11 261 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 108.23 Da
Charge 1.00
Codon Adaptation Index 0.55
Isoelectric point 6.62
Molecular weight 75.44 kDa
Number of residues 697
Modifications

Protein Modifications

Term NameResidueCount
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:19547744
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for trp2 (SPAC19A8.15)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
90298during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
91268during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
96064during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
91095during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
95987during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
77683.15during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
22459.12during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
1.9during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4605
conserved in eukaryotes4515
conserved in bacteria1001
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC19A8.15 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured bypaa1protein phosphatase regulatory subunit Paa1 Affinity Capture-MSPMID:22119525
affinity captured byfar8SIP/FAR complex striatin subunit, Far8/Csc3 Affinity Capture-MSPMID:22119525
External References
Database Identifier Description
NBRP SPAC19A8.15 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC19A8.15 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC19A8.15 BioGRID Interaction Datasets
Expression Viewer SPAC19A8.15 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC19A8.15 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC19A8.15 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC19A8.15 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC19A8.15 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC19A8.15 Transcriptome Viewer (Bähler Lab)
GEO SPAC19A8.15 GEO profiles
PInt SPAC19A8.15 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC19A8.15 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC19A8.15 Fission yeast phenotypic data & analysis
Cyclebase SPAC19A8.15.1 Cell Cycle Data
SPD / RIKEN27/27H01Orfeome Localization Data
IntEnz4.2.1.20Integrated relational Enzyme database
Rhea4.2.1.20Annotated reactions database
UniProtKB/SwissProtO13831Tryptophan synthase
ModBaseO13831Database of comparative protein structure models
STRINGO13831Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593777tryptophan synthase (predicted)
RefSeq mRNANM_001019206972h- tryptophan synthase (predicted) (trp2), mRNA
KEGG00260+4.2.1.20Glycine, serine and threonine metabolism
KEGG00400+4.2.1.20Phenylalanine, tyrosine and tryptophan biosynthesis
UniPathwayUPA00035Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5

Literature for trp2

Search: Europe PMC or PubMed

Release Version: PomBase:30_58 - 11 Mar 2016