pyp2 (SPAC19D5.01)


Gene Standard Namepyp2 Characterisation Statuspublished
Systematic IDSPAC19D5.01 Feature Typeprotein coding
Synonyms Name Description
Producttyrosine phosphatase Pyp2 Product Size711aa, 79.36 kDa
Genomic Location Chromosome I, 5202366-5205202 (2837nt); CDS:5202823-5204958 (2136nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0033550MAP kinase tyrosine phosphatase activity3
has substrate sty1IMPPMID:8649397
GO:0005515protein bindingIPIsty1PMID:8649397831
GO:0004725protein tyrosine phosphatase activityISSUniProtKB:P18031PMID:144808713
inhibited by zinc dichlorideIDAPMID:1464319
inhibited by trisodium vanadateIDAPMID:8256510
inhibited by trisodium vanadateIDAPMID:1464319
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000188inactivation of MAPK activityIMPPMID:86493976
GO:0010972negative regulation of G2/M transition of mitotic cell cycleIGIwee1PMID:144808742
IMPPMID:1464319
GO:0032873negative regulation of stress-activated MAPK cascadeIMPPMID:86493971
GO:0035335peptidyl-tyrosine dephosphorylationISSUniProtKB:P18031PMID:14480878
IMPPMID:8649397
IDAPMID:8256510
GO:1990264peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activityIMPPMID:86493973
GO:0006470protein dephosphorylationIGIsty1PMID:765716435
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmIDAPMID:102331524205
GO:0005829cytosolIDAPMID:168233722315
GO:0005739mitochondrionIDAPMID:16823372758
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assayΔN(1) (1-345)OverexpressionPMID:7937842147
Cell growth assayC630S (C630S)OverexpressionPMID:7937842
Cell growth assayR636K (R636K)OverexpressionPMID:7937842
Cell growth assaypyp2+ (wild type)OverexpressionPMID:7937842
FYPO:0001164normal growth on glucose carbon sourceCell growth assaypyp2ND (462-711)OverexpressionPMID:7937842176
Cell growth assayΔN(2) (1-424)OverexpressionPMID:7937842
Cell growth assaypyp2CD (1-460)OverexpressionPMID:7937842
FYPO:0001420normal vegetative cell population growth rateCell growth assaypyp2ΔNullPMID:146431951
FYPO:0001234slow vegetative cell population growthCell growth assaypyp2+ (wild type)OverexpressionPMID:146431978
Cell growth assaypyp2+ (wild type)OverexpressionPMID:8163491
FYPO:0002060viable vegetative cell populationMicroscopypyp2ΔNullPECO:0000005, PECO:0000137PMID:236978063759
Microscopypyp2ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001962abolished protein phosphatase activity4
affecting pyp2Enzyme assay dataC630S (C630S)Not specifiedPMID:8256510
affecting pyp2Enzyme assay dataΔN(2) (1-424)Not specifiedPECO:0000140PMID:7937842
affecting pyp2Enzyme assay datapyp2CD (1-460)Not specifiedPECO:0000140PMID:7937842
affecting pyp2Enzyme assay dataC630S (C630S)Not specifiedPECO:0000140PMID:7937842
FYPO:0002286decreased RNA level during cellular response to osmotic stress9
affecting gpd1Transcript expression level evidencepyp2ΔNullPMID:8649397
FYPO:0002375decreased protein tyrosine phosphorylation6
affecting sty1Western blot assaypyp2+ (wild type)OverexpressionPMID:8649397
FYPO:0002376decreased protein tyrosine phosphorylation during cellular response to osmotic stress3
affecting sty1Western blot assaypyp2+ (wild type)OverexpressionPMID:8649397
FYPO:0000017elongated cellMicroscopypyp2+ (wild type)OverexpressionPMID:1448087696
FYPO:0001122elongated vegetative cellMicroscopyC630S (C630S)OverexpressionPMID:8649397685
Microscopynot recorded (overexpression)PMID:1448087
FYPO:0000672normal cell morphologyMicroscopypyp2ΔNullPMID:14480873581
FYPO:0000411normal mitotic cell cycleCell growth assaypyp2ΔNullPMID:144808712
FYPO:0001759normal protein phosphatase activity4
affecting pyp2Enzyme assay dataΔN(1) (1-345)Not specifiedPECO:0000140PMID:7937842
FYPO:0002291normal protein tyrosine phosphorylation during cellular response to heat1
affecting sty1Western blot assaypyp2ΔNullPMID:8649397
FYPO:0002290normal protein tyrosine phosphorylation during cellular response to osmotic stress1
affecting sty1Western blot assaypyp2ΔNullPMID:8649397
FYPO:0001315normal vegetative cell morphologyMicroscopypyp2ΔNullPMID:14643193573
FYPO:0001492viable elongated vegetative cellMicroscopypyp2+ (wild type)OverexpressionPMID:7937842221
MicroscopyΔN(1) (1-345)OverexpressionPMID:7937842
MicroscopyC630S (C630S)OverexpressionPMID:7937842
Microscopypyp2+ (wild type)OverexpressionPECO:0000126PMID:1464319
Microscopypyp2+ (wild type)OverexpressionPMID:8163491
MicroscopyR636K (R636K)OverexpressionPMID:7937842
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopypyp2ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in atf1 transcription factor, Atf-CREB family Atf1 PMID:8824587
FYPO affected by mutation in pyp2 tyrosine phosphatase Pyp2 PMID:8256510
FYPO affected by mutation in pyp2 tyrosine phosphatase Pyp2 PMID:7937842
FYPO affected by mutation in sty1 MAP kinase Sty1 PMID:8649397
FYPO affected by mutation in wis1 MAP kinase kinase Wis1 PMID:9718372
FYPO affected by mutation in wis1 MAP kinase kinase Wis1 PMID:8649397
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
152023665205202
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00102 Pfam IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 460 695 3
PF00581 Pfam IPR001763 Rhodanese-like domain 23 123 7
SM00194 SMART IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 421 700 3
SM00450 SMART IPR001763 Rhodanese-like domain 11 127 6
SM00404 SMART IPR003595 Protein-tyrosine phosphatase, catalytic 590 697 6
PS50206 Prosite Profiles IPR001763 Rhodanese-like domain 21 130 7
PS50055 Prosite Profiles IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 461 698 3
PS50056 Prosite Profiles IPR000387 Protein-tyrosine/Dual specificity phosphatase 608 689 6
PS00383 Prosite Patterns IPR016130 Protein-tyrosine phosphatase, active site 628 638 7
PTHR19134:SF104 HMMPANTHER 27 695 1
PTHR19134 HMMPANTHER 27 695 3
3.90.190.10 Gene3D 456 700 9
3.40.250.10 Gene3D IPR001763 Rhodanese-like domain 4 130 8
SSF52821 SuperFamily IPR001763 Rhodanese-like domain 26 127 7
SSF52799 SuperFamily Protein-tyrosine phosphatase-like 454 697 9
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 589 606 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 499 519 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 625 643 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 665 680 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 485 492 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 681 691 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.61 Da
Charge 24.00
Isoelectric point 8.89
Molecular weight 79.36 kDa
Number of residues 711
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS48PMID:217125471670
present during mitotic M phase
MOD:00047O-phospho-L-threonineexperimental evidenceT51PMID:21712547692
present during mitotic M phase
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0070301Northern assay evidencePMID:8649397
present during GO:0072690Northern assay evidencePMID:1464319
increased during GO:0071470Northern assay evidencePMID:8649397
increased during GO:0034605Northern assay evidencePMID:8649397
increased during GO:0071470expression microarray evidencePMID:12529438
increased during GO:0071470expression microarray evidencePMID:12529438
increased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased during GO:0071472Northern assay evidencePMID:8824587
present during GO:0072690Northern assay evidencePMID:8649397
increased during GO:0034599expression microarray evidencePMID:12529438
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438
increased during GO:0034605expression microarray evidencePMID:12529438

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.95during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.14during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
pmc1mediator complex subunit Pmc1 Dosage RescuePMID:23271606
scs7sphingosine hydroxylase Scs7 Positive GeneticPMID:22681890
ucp12ATP-dependent RNA helicase Ucp12 (predicted) Negative GeneticPMID:22681890
vps17retromer complex subunit Vps17 Synthetic Growth DefectPMID:18931302
apm4AP-2 adaptor complex subunit Apm4 (predicted) Negative GeneticPMID:22681890
SPAC18B11.02ctRNA pseudouridine synthase (predicted) Negative GeneticPMID:22681890
dli1meiotic dynein intermediate light chain Dli1/Dil1 Negative GeneticPMID:22681890
ubp3ubiquitin C-terminal hydrolase Ubp3 Phenotypic SuppressionPMID:19547744
mso1endocytic docking protein Mso1 Negative GeneticPMID:22681890
cdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Dosage LethalityPMID:7937842
pyp1tyrosine phosphatase Pyp1 Synthetic LethalityPMID:1448087
Synthetic LethalityPMID:1464319
SPBC2F12.05csterol binding ankyrin repeat protein (predicted) Negative GeneticPMID:22681890
wtf21wtf element Wtf21 Negative GeneticPMID:22681890
rps340S ribosomal protein S3 (predicted) Negative GeneticPMID:22681890
csn3COP9/signalosome complex subunit Csn3 (predicted) Positive GeneticPMID:22681890
moc3transcription factor Moc3 Positive GeneticPMID:22681890
SPCC594.01DUF1769 family protein Negative GeneticPMID:22681890
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Dosage RescuePMID:23271606
wee1M phase inhibitor protein kinase Wee1 Phenotypic SuppressionPMID:1448087
SPAC6B12.07cubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
pvg2galactose residue biosynthesis protein Pvg2 Negative GeneticPMID:22681890
dbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
rfp2SUMO-targeted ubiquitin-protein ligase subunit Rfp2 Positive GeneticPMID:22681890
trm13tRNA 2'-O-methyltransferase Trm13 (predicted) Negative GeneticPMID:22681890
ckb2CK2 family regulatory subunit Ckb2 (predicted) Negative GeneticPMID:22681890
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic SuppressionPMID:1448087
sty1MAP kinase Sty1 Phenotypic SuppressionPMID:7501024
uge1UDP-glucose 4-epimerase Uge1 Negative GeneticPMID:22681890
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
gld1mitochondrial glycerol dehydrogenase Gld1 Negative GeneticPMID:22681890
mto1MT organizer Mto1 Positive GeneticPMID:22681890
cdc25M phase inducer tyrosine phosphatase Cdc25 Synthetic RescuePMID:1464319
Phenotypic Enhancement
Dosage LethalityPMID:7937842
slm9hira protein Slm9 Synthetic Growth DefectPMID:18931302
mug51variant protein kinase 19 family protein Negative GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
acp1F-actin capping protein alpha subunit Negative GeneticPMID:22681890
git5heterotrimeric G protein beta subunit Git5 Negative GeneticPMID:22681890
SPCC1281.04pyridoxal reductase (predicted) Negative GeneticPMID:22681890
pka1cAMP-dependent protein kinase catalytic subunit Pka1 Phenotypic SuppressionPMID:8832414
ryh1GTPase Ryh1 Synthetic Growth DefectPMID:18931302
Dosage RescuePMID:23271606
uds1septation protein Uds1 Negative GeneticPMID:22681890
swc2Swr1 complex complex subunit Swc2 Negative GeneticPMID:22681890
SPAC1805.14Schizosaccharomyces specific protein Negative GeneticPMID:22681890
gmh4alpha-1,2-galactosyltransferase (predicted) Negative GeneticPMID:22681890
mug147Schizosaccharomyces specific protein Mug147 Negative GeneticPMID:22681890
vps38phophatidylinositol 3-kinase complex subunit Vps38 Negative GeneticPMID:22681890
hmt2sulfide-quinone oxidoreductase Negative GeneticPMID:22681890
SPAC17H9.08mitochondrial coenzyme A transporter (predicted) Negative GeneticPMID:22681890
zwf2glucose-6-phosphate 1-dehydrogenase Zwf2 (predicted) Positive GeneticPMID:22681890
SPAC31A2.12arrestin/PY protein 1 (predicted) Negative GeneticPMID:22681890
SPBC32F12.12cGolgi membrane protein involved in vesicle-mediated transport (predicted) Negative GeneticPMID:22681890
SPCP1E11.10ankyrin repeat protein, unknown biological role Negative GeneticPMID:22681890
SPAC18G6.12cThiJ domain protein (predicted) Negative GeneticPMID:22681890
SPBC3F6.01cserine/threonine protein phosphatase (predicted) Negative GeneticPMID:22681890
rps40340S ribosomal protein S4 (predicted) Negative GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
spa1ornithine decarboxylase antizyme with +1 programmed ribosomal frameshift Spa1 Positive GeneticPMID:22681890
cyr1adenylate cyclase Phenotypic SuppressionPMID:8832414
pac10prefoldin subunit 3 Pac10 (predicted) Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
sty1MAP kinase Sty1 Biochemical ActivityPMID:23690545
Affinity Capture-MS
Biochemical ActivityPMID:7657164
Affinity Capture-RNAPMID:22144913
Affinity Capture-WesternPMID:8649397
Biochemical Activity
Two-hybridPMID:23695164
wee1M phase inhibitor protein kinase Wee1 Biochemical ActivityPMID:8256510
External References
Database Identifier Description
NBRP SPAC19D5.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC19D5.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC19D5.01 BioGRID Interaction Datasets
Expression Viewer SPAC19D5.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC19D5.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC19D5.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC19D5.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC19D5.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC19D5.01 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC19D5.01 Cell Cycle Data
GEO SPAC19D5.01 GEO profiles
PInt SPAC19D5.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC19D5.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC19D5.01 Fission yeast phenotypic data & analysis
IntEnz3.1.3.48Integrated relational Enzyme database
Rhea3.1.3.48Annotated reactions database
SPD / RIKEN37/37E03Orfeome Localization Data
UniProtKB/SwissProtP32586Tyrosine-protein phosphatase 2
ModBaseP32586Database of comparative protein structure models
STRINGP32586Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594899tyrosine phosphatase Pyp2
RefSeq mRNANM_001020328972h- tyrosine phosphatase Pyp2 (pyp2), mRNA
European Nucleotide ArchiveX59599ENA EMBL mapping
European Nucleotide ArchiveAAB24544.1ENA Protein Mapping
European Nucleotide ArchiveBAA87093.1ENA Protein Mapping
European Nucleotide ArchiveCAB16711.1ENA Protein Mapping
UniParcUPI0000132BF8UniProt Archive

Literature for pyp2

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014