pyp2 (SPAC19D5.01)


Gene Standard Namepyp2 Characterisation Statuspublished
Systematic IDSPAC19D5.01 Feature Typeprotein coding
Synonyms Name Description
Producttyrosine phosphatase Pyp2 Product Size711aa, 79.36 kDa
Genomic Location Chromosome I, 5202366-5205202 (2837nt); CDS:5202823-5204958 (2136nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
MAP kinase tyrosine phosphatase activity3
Annotation ExtensionEvidenceWith/FromReference
protein binding854
Annotation ExtensionEvidenceWith/FromReference
protein tyrosine phosphatase activity11
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytoplasm4208
Annotation ExtensionEvidenceWith/FromReference
cytosol2315
Annotation ExtensionEvidenceWith/FromReference
mitochondrion761
Annotation ExtensionEvidenceWith/FromReference
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth on glucose carbon sourceΔN(1) (1-345)Overexpression247
C630S (C630S)Overexpression
pyp2+ (wild type)Overexpression
R636K (R636K)Overexpression
normal growth on glucose carbon sourceΔN(2) (1-424)Overexpression200
pyp2CD (1-460)Overexpression
pyp2ND (462-711)Overexpression
normal vegetative cell population growth ratepyp2ΔNull61
slow vegetative cell population growthpyp2+ (wild type)Overexpression321
pyp2ΔNull
viable vegetative cell populationpyp2ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
abolished protein phosphatase activity4
affecting pyp2ΔN(2) (1-424)Not specified
affecting pyp2C630S (C630S)Not specified
affecting pyp2pyp2CD (1-460)Not specified
decreased protein tyrosine phosphorylation10
affecting sty1pyp2+ (wild type)Overexpression
decreased protein tyrosine phosphorylation during cellular response to osmotic stress4
affecting sty1pyp2+ (wild type)Overexpression
decreased RNA level during cellular response to osmotic stress13
affecting gpd1pyp2ΔNull
elongated vegetative cellC630S (C630S)Overexpression752
pyp2+ (wild type)Overexpression
increased duration of protein phosphorylation during cellular response to salt stress4
affecting sty1pyp2ΔNull
increased protein phosphorylation during cellular response to hydrogen peroxide3
affecting sty1pyp2ΔNull
normal mitotic cell cyclepyp2ΔNull81
normal protein phosphatase activity4
affecting pyp2ΔN(1) (1-345)Not specified
normal protein tyrosine phosphorylation during cellular response to heat1
affecting sty1pyp2ΔNull
normal protein tyrosine phosphorylation during cellular response to osmotic stress4
affecting sty1pyp2ΔNull
normal vegetative cell morphologypyp2ΔNull3581
viable elongated vegetative cellΔN(1) (1-345)Overexpression283
C630S (C630S)Overexpression
pyp2+ (wild type)Overexpression
R636K (R636K)Overexpression
viable vegetative cell with normal cell morphologypyp2ΔNull3092
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in atf1 transcription factor, Atf-CREB family Atf1 PMID:8824588
FYPO affected by mutation in atf1 transcription factor, Atf-CREB family Atf1 PMID:8824587
FYPO affected by mutation in mcs4 response regulator Mcs4 PMID:9136929
FYPO affected by mutation in pim1 RCC1 family Ran GDP/GTP exchange factor PMID:10233152
FYPO affected by mutation in pyp2 tyrosine phosphatase Pyp2 PMID:7937842
FYPO affected by mutation in pyp2 tyrosine phosphatase Pyp2 PMID:8256510
FYPO affected by mutation in sty1 MAP kinase Sty1 PMID:9136929
FYPO affected by mutation in sty1 MAP kinase Sty1 PMID:8649397
FYPO affected by mutation in win1 MAP kinase kinase kinase Win1 PMID:9450957
FYPO affected by mutation in wis1 MAP kinase kinase Wis1 PMID:8649397
FYPO affected by mutation in wis1 MAP kinase kinase Wis1 PMID:9718372
FYPO affected by mutation in wis4 MAP kinase kinase kinase Wis4 PMID:9450957
FYPO affected by mutation in wis4 MAP kinase kinase kinase Wis4 PMID:9136929
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons5202366..5205202
mRNA5202366..5205202
5' UTR5202366..5202822SPPYP2
CDS5202823..5204958
3' UTR5204959..5205202SPC03110
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00581 Pfam IPR001763 Rhodanese-like domain 23 123 7
PF00102 Pfam IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 460 695 3
SM00404 SMART IPR003595 Protein-tyrosine phosphatase, catalytic 590 697 6
SM00450 SMART IPR001763 Rhodanese-like domain 11 127 6
SM00194 SMART IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 421 700 3
PS00383 Prosite Patterns IPR016130 Protein-tyrosine phosphatase, active site 628 638 7
PS50055 Prosite Profiles IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 461 698 3
PS50056 Prosite Profiles IPR000387 Protein-tyrosine/Dual specificity phosphatase 608 689 6
PS50206 Prosite Profiles IPR001763 Rhodanese-like domain 21 130 7
PTHR19134 HMMPANTHER 460 695 3
3.90.190.10 Gene3D IPR029021 Protein-tyrosine phosphatase-like 456 700 9
3.40.250.10 Gene3D IPR001763 Rhodanese-like domain 4 130 8
SSF52821 SuperFamily IPR001763 Rhodanese-like domain 26 127 7
SSF52799 SuperFamily IPR029021 Protein-tyrosine phosphatase-like 454 697 9
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 499 519 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 625 643 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 665 680 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 589 606 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 681 691 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 485 492 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.61 Da
Charge 24.00
Isoelectric point 8.89
Molecular weight 79.36 kDa
Number of residues 711
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1672
present during mitotic M phaseS48
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S48 PMID:21712547
O-phospho-L-threonine 698
present during mitotic M phaseT51
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T51 PMID:21712547
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelabsent during GO:0072690Northern assay evidencePMID:9450957
increased during GO:0070301Northern assay evidencePMID:8824588
increased during GO:0072709Northern assay evidencePMID:8824588
increased during GO:0034605Northern assay evidencePMID:8824588
increased during GO:0071472Northern assay evidencePMID:8824588
increased during GO:0070301Northern assay evidencePMID:9450957
increased during GO:0071472Northern assay evidencePMID:8824587
increased during GO:0070301Northern assay evidencePMID:8649397
present during GO:0072690Northern assay evidencePMID:1464319
increased during GO:0071470Northern assay evidencePMID:8649397
increased during GO:0034605Northern assay evidencePMID:8649397
present during GO:0072690Northern assay evidencePMID:8649397
increased during GO:0034605Northern assay evidencePMID:9450957
increased during GO:0072740Northern assay evidencePMID:9450957
increased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased during GO:0071470expression microarray evidencePMID:12529438
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438
increased during GO:0071472Northern assay evidencePMID:9450957
increased during GO:0034605expression microarray evidencePMID:12529438
increased during GO:0071470expression microarray evidencePMID:12529438
increased during GO:0034599expression microarray evidencePMID:12529438

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.95during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.14during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
modifiessty1MAP kinase Sty1 Biochemical ActivityPMID:23690545
affinity capturessty1MAP kinase Sty1 Affinity Capture-MSPMID:23690545
modifiessty1MAP kinase Sty1 Biochemical ActivityPMID:7657164
affinity captured bysty1MAP kinase Sty1 Affinity Capture-RNAPMID:22144913
modifiessty1MAP kinase Sty1 Biochemical ActivityPMID:8649397
affinity capturessty1MAP kinase Sty1 Affinity Capture-WesternPMID:8649397
binds activation domain construct withsty1MAP kinase Sty1 Two-hybridPMID:23695164
modifieswee1M phase inhibitor protein kinase Wee1 Biochemical ActivityPMID:8256510
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withacp1F-actin capping protein alpha subunit Negative GeneticPMID:22681890
negative genetic interaction withapm4AP-2 adaptor complex subunit Apm4 (predicted) Negative GeneticPMID:22681890
overexpression rescuesbgs11,3-beta-glucan synthase catalytic subunit Bgs1 Dosage RescuePMID:23271606
rescued bycdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic SuppressionPMID:1448087
synthetically rescuescdc25M phase inducer tyrosine phosphatase Cdc25 Synthetic RescuePMID:1464319
enhances phenotype ofcdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic EnhancementPMID:1464319
overexpression lethal tocdc25M phase inducer tyrosine phosphatase Cdc25 Dosage LethalityPMID:7937842
overexpression lethal tocdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Dosage LethalityPMID:7937842
negative genetic interaction withckb2CK2 family regulatory subunit Ckb2 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withcsn3COP9/signalosome complex subunit Csn3 (predicted) Positive GeneticPMID:22681890
rescuescyr1adenylate cyclase Phenotypic SuppressionPMID:8832414
negative genetic interaction withdbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
negative genetic interaction withdli1meiotic dynein intermediate light chain Dli1/Dil1 Negative GeneticPMID:22681890
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withgit5heterotrimeric G protein beta subunit Git5 Negative GeneticPMID:22681890
negative genetic interaction withgld1mitochondrial glycerol dehydrogenase Gld1 Negative GeneticPMID:22681890
negative genetic interaction withgmh4alpha-1,2-galactosyltransferase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhmt2sulfide-quinone oxidoreductase Negative GeneticPMID:22681890
positive genetic interaction withmoc3transcription factor Moc3 Positive GeneticPMID:22681890
negative genetic interaction withmso1endocytic docking protein Mso1 Negative GeneticPMID:22681890
positive genetic interaction withmto1MT organizer Mto1 Positive GeneticPMID:22681890
negative genetic interaction withmug147Schizosaccharomyces specific protein Mug147 Negative GeneticPMID:22681890
negative genetic interaction withmug51variant protein kinase 19 family protein Negative GeneticPMID:22681890
negative genetic interaction withpac10prefoldin subunit 3 Pac10 (predicted) Negative GeneticPMID:22681890
rescuespka1cAMP-dependent protein kinase catalytic subunit Pka1 Phenotypic SuppressionPMID:8832414
overexpression rescuespmc1mediator complex subunit Pmc1 Dosage RescuePMID:23271606
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withpvg2galactose residue biosynthesis protein Pvg2 Negative GeneticPMID:22681890
synthetic lethal withpyp1tyrosine phosphatase Pyp1 Synthetic LethalityPMID:1448087
synthetic lethal withpyp1tyrosine phosphatase Pyp1 Synthetic LethalityPMID:1464319
positive genetic interaction withrfp2SUMO-targeted ubiquitin-protein ligase subunit Rfp2 Positive GeneticPMID:22681890
negative genetic interaction withrps340S ribosomal protein S3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrps40340S ribosomal protein S4 (predicted) Negative GeneticPMID:22681890
synthetic growth defect withryh1GTPase Ryh1 Synthetic Growth DefectPMID:18931302
overexpression rescuesryh1GTPase Ryh1 Dosage RescuePMID:23271606
positive genetic interaction withscs7sphingosine hydroxylase Scs7 Positive GeneticPMID:22681890
synthetic growth defect withslm9hira protein Slm9 Synthetic Growth DefectPMID:18931302
positive genetic interaction withspa1ornithine decarboxylase antizyme with +1 programmed ribosomal frameshift Spa1 Positive GeneticPMID:22681890
negative genetic interaction withSPAC17H9.08mitochondrial coenzyme A transmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC1805.14Schizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withSPAC18B11.02ctRNA pseudouridine synthase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC18G6.12cThiJ domain protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC31A2.12arrestin/PY protein 1 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withSPAC6B12.07cubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
negative genetic interaction withSPBC2F12.05csterol binding ankyrin repeat protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC32F12.12cGolgi membrane protein involved in vesicle-mediated transport (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC3F6.01cserine/threonine protein phosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC1281.04pyridoxal reductase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC594.01DUF1769 family protein Negative GeneticPMID:22681890
negative genetic interaction withSPCP1E11.10ankyrin repeat protein, unknown biological role Negative GeneticPMID:22681890
rescued bysty1MAP kinase Sty1 Phenotypic SuppressionPMID:7501024
negative genetic interaction withswc2Swr1 complex complex subunit Swc2 Negative GeneticPMID:22681890
negative genetic interaction withtrm13tRNA 2'-O-methyltransferase Trm13 (predicted) Negative GeneticPMID:22681890
rescuesubp3ubiquitin C-terminal hydrolase Ubp3 Phenotypic SuppressionPMID:19547744
negative genetic interaction withucp12ATP-dependent RNA helicase Ucp12 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withuds1septation protein Uds1 Negative GeneticPMID:22681890
negative genetic interaction withuge1UDP-glucose 4-epimerase Uge1 Negative GeneticPMID:22681890
synthetic growth defect withvps17retromer complex subunit Vps17 Synthetic Growth DefectPMID:18931302
negative genetic interaction withvps38phophatidylinositol 3-kinase complex subunit Vps38 Negative GeneticPMID:22681890
rescued bywee1M phase inhibitor protein kinase Wee1 Phenotypic SuppressionPMID:1448087
negative genetic interaction withwtf21wtf element Wtf21 Negative GeneticPMID:22681890
positive genetic interaction withzwf2glucose-6-phosphate 1-dehydrogenase Zwf2 (predicted) Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC19D5.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC19D5.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC19D5.01 BioGRID Interaction Datasets
Expression Viewer SPAC19D5.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC19D5.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC19D5.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC19D5.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC19D5.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC19D5.01 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC19D5.01 Cell Cycle Data
GEO SPAC19D5.01 GEO profiles
PInt SPAC19D5.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC19D5.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC19D5.01 Fission yeast phenotypic data & analysis
IntEnz3.1.3.48Integrated relational Enzyme database
Rhea3.1.3.48Annotated reactions database
SPD / RIKEN37/37E03Orfeome Localization Data
UniProtKB/SwissProtP32586Tyrosine-protein phosphatase 2
ModBaseP32586Database of comparative protein structure models
STRINGP32586Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594899tyrosine phosphatase Pyp2
RefSeq mRNANM_001020328972h- tyrosine phosphatase Pyp2 (pyp2), mRNA
European Nucleotide ArchiveX59599ENA EMBL mapping
European Nucleotide ArchiveAAB24544.1ENA Protein Mapping
European Nucleotide ArchiveBAA87093.1ENA Protein Mapping
European Nucleotide ArchiveCAB16711.1ENA Protein Mapping
UniParcUPI0000132BF8UniProt Archive

Literature for pyp2

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015