pyp2 (SPAC19D5.01)


Gene Standard Namepyp2 Characterisation Statuspublished
Systematic IDSPAC19D5.01 Feature Typeprotein coding
Synonyms Name Description
Producttyrosine phosphatase Pyp2 Product Size711aa, 79.36 kDa
Genomic Location Chromosome I, 5202366-5205202 (2837nt); CDS:5202823-5204958 (2136nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0033550MAP kinase tyrosine phosphatase activity3
has substrate sty1IMPPMID:8649397
GO:0005515protein bindingIPIsty1PMID:8649397887
GO:0004725protein tyrosine phosphatase activityISSUniProtKB:P18031PMID:144808713
inhibited by trisodium vanadateIDAPMID:1464319
has substrate sty1TASPMID:7657164
inhibited by zinc dichlorideIDAPMID:1464319
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000188inactivation of MAPK activityIMPPMID:86493975
GO:0010972negative regulation of G2/M transition of mitotic cell cycleIGIwee1PMID:144808750
IMPPMID:1464319
GO:0045835negative regulation of meiosisIMPPMID:16688856
GO:0032873negative regulation of stress-activated MAPK cascadeIMPPMID:86493971
GO:0035335peptidyl-tyrosine dephosphorylationISSUniProtKB:P18031PMID:14480878
IMPPMID:8649397
GO:1990264peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activityIMPPMID:86493973
GO:0006470protein dephosphorylationIGIsty1PMID:765716435
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmIDAPMID:102331524198
GO:0005829cytosolIDAPMID:168233722317
GO:0005739mitochondrionIDAPMID:16823372756
GO:0005634nucleusIDAPMID:168233722730
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001420normal vegetative cell population growth rateCell growth assaypyp2ΔNullPMID:146431929
FYPO:0001234slow vegetative cell population growthCell growth assaypyp2+ (wild type)OverexpressionPMID:816349140
Cell growth assaypyp2+ (wild type)OverexpressionPMID:1464319
FYPO:0002060viable vegetative cell populationMicroscopypyp2ΔNullPECO:0000005, PECO:0000137PMID:236978063755
Microscopypyp2ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002286decreased RNA level during cellular response to osmotic stressTranscript expression level evidencepyp2ΔNullPMID:86493974
affecting gpd1
FYPO:0002375decreased protein tyrosine phosphorylationWestern blot assaypyp2+ (wild type)OverexpressionPMID:86493972
affecting sty1
FYPO:0002376decreased protein tyrosine phosphorylation during cellular response to osmotic stressWestern blot assaypyp2+ (wild type)OverexpressionPMID:86493972
affecting sty1
FYPO:0000017elongated cellMicroscopypyp2+ (wild type)OverexpressionPMID:1448087408
FYPO:0001122elongated vegetative cellMicroscopyC630S (C630S)OverexpressionPMID:8649397624
not recorded (overexpression)PMID:1448087
FYPO:0000411normal cell cycleCell growth assaypyp2ΔNullPMID:144808724
FYPO:0000672normal cell morphologyMicroscopypyp2ΔNullPMID:144808755
FYPO:0002291normal protein tyrosine phosphorylation during cellular response to heatWestern blot assaypyp2ΔNullPMID:86493971
affecting sty1
FYPO:0002290normal protein tyrosine phosphorylation during cellular response to osmotic stressWestern blot assaypyp2ΔNullPMID:86493971
affecting sty1
FYPO:0001315normal vegetative cell morphologyMicroscopypyp2ΔNullPMID:146431942
FYPO:0001492viable elongated vegetative cellMicroscopypyp2+ (wild type)OverexpressionPMID:8163491170
Microscopypyp2+ (wild type)OverexpressionPECO:0000126PMID:1464319
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopypyp2ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
152023665205202
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00581 Pfam IPR001763 Rhodanese-like domain 23 123 7
PF00102 Pfam IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 460 695 3
SM00194 SMART IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 421 700 3
SM00450 SMART IPR001763 Rhodanese-like domain 11 127 6
PS00383 Prosite Patterns IPR016130 Protein-tyrosine phosphatase, active site 628 638 7
PS50206 Prosite Profiles IPR001763 Rhodanese-like domain 21 130 7
PS50055 Prosite Profiles IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 461 698 3
PS50056 Prosite Profiles IPR000387 Protein-tyrosine/Dual specificity phosphatase 608 689 6
PTHR19134 HMMPANTHER 27 695 3
PTHR19134:SF104 HMMPANTHER 27 695 1
G3DSA:3.40.250.10 Gene3D IPR001763 4 130 8
G3DSA:3.90.190.10 Gene3D 456 700 8
SSF52799 SuperFamily 454 697 9
SSF52821 SuperFamily IPR001763 26 127 7
Low complexity (SEG) seg 248 261
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 589 606 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 665 680 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 681 691 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 499 519 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 485 492 3
PR00700 PRINTS IPR000242 Protein-tyrosine phosphatase, receptor/non-receptor type 625 643 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.61 Da
Charge 24.00
Isoelectric point 8.89
Molecular weight 79.36 kDa
Number of residues 711
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreasedexpression microarray evidencePMID:12529438
during GO:0034599
increasedNorthern assay evidencePMID:8649397
during GO:0034605
increased,
in presence of cadmium sulfate
expression microarray evidencePMID:12529438
increasedexpression microarray evidencePMID:12529438
during GO:0034605
increasedexpression microarray evidencePMID:12529438
during GO:0071470
increased,
in presence of methyl methanesulfonate
expression microarray evidencePMID:12529438
increasedexpression microarray evidencePMID:12529438
during GO:0071470
increasedNorthern assay evidencePMID:8649397
during GO:0071470
presentNorthern assay evidencePMID:1464319
during GO:0072690
increasedNorthern assay evidencePMID:8649397
during GO:0070301
presentNorthern assay evidencePMID:8649397
during GO:0072690

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.95during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.14during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
pmc1mediator complex subunit Pmc1 Dosage RescuePMID:23271606
scs7sphingosine hydroxylase Scs7 Positive GeneticPMID:22681890
ucp12ATP-dependent RNA helicase Ucp12 (predicted) Negative GeneticPMID:22681890
vps17retromer complex subunit Vps17 Synthetic Growth DefectPMID:18931302
apm4AP-2 adaptor complex subunit Apm4 (predicted) Negative GeneticPMID:22681890
SPAC18B11.02ctRNA pseudouridine synthase (predicted) Negative GeneticPMID:22681890
dli1meiotic dynein intermediate light chain Dli1/Dil1 Negative GeneticPMID:22681890
ubp3ubiquitin C-terminal hydrolase Ubp3 Phenotypic SuppressionPMID:19547744
SPAC1F5.05cconserved fungal protein Negative GeneticPMID:22681890
csn3COP9/signalosome complex subunit Csn3 (predicted) Positive GeneticPMID:22681890
pyp1tyrosine phosphatase Pyp1 Synthetic LethalityPMID:1448087
Synthetic LethalityPMID:1464319
SPBC2F12.05csterol binding ankyrin repeat protein (predicted) Negative GeneticPMID:22681890
wtf21wtf element Wtf21 Negative GeneticPMID:22681890
rps340S ribosomal protein S3 (predicted) Negative GeneticPMID:22681890
moc3transcription factor Moc3 Positive GeneticPMID:22681890
SPCC594.01DUF1769 family protein Negative GeneticPMID:22681890
bgs11,3-beta-glucan synthase catalytic subunit Bgs1 Dosage RescuePMID:23271606
wee1M phase inhibitor protein kinase Wee1 Phenotypic SuppressionPMID:1448087
SPAC6B12.07cubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
pvg2galactose residue biosynthesis protein Pvg2 Negative GeneticPMID:22681890
dbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
rfp2SUMO-targeted ubiquitin-protein ligase subunit Rfp2 Positive GeneticPMID:22681890
trm13tRNA 2'-O-methyltransferase Trm13 (predicted) Negative GeneticPMID:22681890
ckb2CK2 family regulatory subunit (predicted) Negative GeneticPMID:22681890
cdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic SuppressionPMID:1448087
sty1MAP kinase Sty1 Phenotypic SuppressionPMID:7501024
uge1UDP-glucose 4-epimerase Uge1 Negative GeneticPMID:22681890
dsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
gld1mitochondrial glycerol dehydrogenase Gld1 Negative GeneticPMID:22681890
mto1MT organizer Mto1 Positive GeneticPMID:22681890
cdc25M phase inducer phosphatase Cdc25 Phenotypic EnhancementPMID:1464319
Synthetic Rescue
Dosage LethalityPMID:7937842
slm9hira protein Slm9 Synthetic Growth DefectPMID:18931302
mug51variant protein kinase 19 family protein Negative GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
acp1F-actin capping protein alpha subunit Negative GeneticPMID:22681890
git5heterotrimeric G protein beta subunit Git5 Negative GeneticPMID:22681890
SPCC1281.04pyridoxal reductase (predicted) Negative GeneticPMID:22681890
pka1cAMP-dependent protein kinase catalytic subunit Pka1 Phenotypic SuppressionPMID:8832414
ryh1GTPase Ryh1 Synthetic Growth DefectPMID:18931302
Dosage RescuePMID:23271606
uds1septation protein Uds1 Negative GeneticPMID:22681890
swc2Swr1 complex complex subunit Swc2 Negative GeneticPMID:22681890
SPAC1805.14sequence orphan Negative GeneticPMID:22681890
SPBC8D2.17alpha-1,2-galactosyltransferase (predicted) Negative GeneticPMID:22681890
mug147sequence orphan Negative GeneticPMID:22681890
vps38phophatidylinositol 3-kinase complex subunit Vps38 Negative GeneticPMID:22681890
hmt2sulfide-quinone oxidoreductase Negative GeneticPMID:22681890
SPAC17H9.08mitochondrial coenzyme A transporter (predicted) Negative GeneticPMID:22681890
SPCC794.01cglucose-6-phosphate 1-dehydrogenase (predicted) Positive GeneticPMID:22681890
SPAC31A2.12arrestin/PY protein 1 (predicted) Negative GeneticPMID:22681890
SPBC32F12.12cGolgi membrane protein involved in vesicle-mediated transport (predicted) Negative GeneticPMID:22681890
SPCP1E11.10ankyrin repeat protein, unknown biological role Negative GeneticPMID:22681890
SPAC18G6.12chypothetical protein Negative GeneticPMID:22681890
SPBC3F6.01cserine/threonine protein phosphatase (predicted) Negative GeneticPMID:22681890
rps40340S ribosomal protein S4 (predicted) Negative GeneticPMID:22681890
prz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
spa1ornithine decarboxylase antizyme with +1 programmed ribosomal frameshift Spa1 Positive GeneticPMID:22681890
cyr1adenylate cyclase Phenotypic SuppressionPMID:8832414
pac10prefoldin subunit 3 Pac10 (predicted) Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
sty1MAP kinase Sty1 Affinity Capture-MSPMID:23690545
Biochemical Activity
Biochemical ActivityPMID:7657164
Affinity Capture-RNAPMID:22144913
Biochemical ActivityPMID:8649397
Affinity Capture-Western
Two-hybridPMID:23695164
wee1M phase inhibitor protein kinase Wee1 Biochemical ActivityPMID:8256510
External References
Database Identifier Description
NBRP SPAC19D5.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC19D5.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC19D5.01 BioGRID Interaction Datasets
Expression Viewer SPAC19D5.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC19D5.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC19D5.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC19D5.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC19D5.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC19D5.01 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC19D5.01 Cell Cycle Data
GEO SPAC19D5.01 GEO profiles
PInt SPAC19D5.01 Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542200tyrosine phosphatase Pyp2
EntrezGene2542200tyrosine phosphatase Pyp2
IntEnz3.1.3.48Integrated relational Enzyme database
Rhea3.1.3.48Annotated reactions database
SPD / RIKEN37/37E03Orfeome Localization Data
UniProtKB/SwissProtP32586Tyrosine-protein phosphatase 2
ModBaseP32586Database of comparative protein structure models
StringP32586Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594899tyrosine phosphatase Pyp2
RefSeq mRNANM_001020328972h- tyrosine phosphatase Pyp2 (pyp2), mRNA
European Nucleotide ArchiveAAB24544ENA Protein Mapping
European Nucleotide ArchiveBAA87093ENA Protein Mapping
European Nucleotide ArchiveCAA42167ENA Protein Mapping
European Nucleotide ArchiveCAB16711ENA Protein Mapping
UniParcUPI0000132BF8UniProt Archive

Literature for pyp2

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014