pyp2 (SPAC19D5.01)


Gene Standard Namepyp2 Characterisation Statuspublished
Systematic IDSPAC19D5.01 Feature Typeprotein coding
Synonyms Name Description
Producttyrosine phosphatase Pyp2 Product Size711aa, 79.36 kDa
Genomic Location Chromosome I, 5202366-5205202 (2837nt); CDS:5202823-5204958 (2136nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
MAP kinase phosphatase activity involved in regulation of osmosensory signaling pathway1
Annotation ExtensionEvidenceWith/FromReference
MAP kinase tyrosine phosphatase activity3
Annotation ExtensionEvidenceWith/FromReference
protein binding862
Annotation ExtensionEvidenceWith/FromReference
protein tyrosine phosphatase activity13
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
GO Cellular Component
Term NameCount
cytoplasm4209
Annotation ExtensionEvidenceWith/FromReference
cytosol2306
Annotation ExtensionEvidenceWith/FromReference
mitochondrion752
Annotation ExtensionEvidenceWith/FromReference
nucleus2690
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth on glucose carbon sourceC630S (C630S)293
pyp2Δ
pyp2+ (wild type)
ΔN(1) (1-345)
R636K (R636K)
normal growth on glucose carbon sourcepyp2ND (462-711)263
ΔN(2) (1-424)
pyp2CD (1-460)
normal vegetative cell population growth ratepyp2Δ79
slow vegetative cell population growthpyp2+ (wild type)340
pyp2Δ
viable vegetative cell populationpyp2Δ3831

Cell Phenotype

Term NameGenotypesCount
abolished cell cycle arrest in mitotic G1 phase in response to nitrogen starvationpyp2Δ6
abolished protein phosphatase activity4
affecting pyp2pyp2CD (1-460)
affecting pyp2ΔN(2) (1-424)
affecting pyp2C630S (C630S)
abolished transcriptional response to pheromonepyp2Δ7
decreased protein tyrosine phosphorylation10
affecting sty1pyp2+ (wild type)
decreased protein tyrosine phosphorylation during cellular response to osmotic stress4
affecting sty1pyp2+ (wild type)
decreased RNA level during cellular response to osmotic stress15
affecting gpd1pyp2Δ
elongated vegetative cellC630S (C630S)787
pyp2+ (wild type)
increased duration of protein phosphorylation during cellular response to salt stress4
affecting sty1pyp2Δ
increased duration of protein tyrosine phosphorylation during cellular response to salt stress1
affecting sty1pyp2Δ
increased protein phosphorylation during cellular response to hydrogen peroxide3
affecting sty1pyp2Δ
normal mitotic cell cyclepyp2Δ109
normal protein phosphatase activity4
affecting pyp2ΔN(1) (1-345)
normal protein tyrosine phosphorylation during cellular response to heat2
affecting sty1pyp2Δ
normal protein tyrosine phosphorylation during cellular response to osmotic stress4
affecting sty1pyp2Δ
normal vegetative cell morphologypyp2Δ562
viable elongated vegetative cellpyp2+ (wild type)313
C630S (C630S)
ΔN(1) (1-345)
R636K (R636K)
viable small vegetative cellpyp2Δ49
viable vegetative cell with normal cell morphologypyp2Δ3099

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
inviable vegetative cell populationpyp2Δ, pyp1+
pyp2+ (wild type), cdr1Δ
viable vegetative cell populationpyp1Δ, sty2-1, pyp2Δ
pyp1Δ, wis1Δ, pyp2Δ
pyp1Δ, sty1-1, pyp2Δ
pyp2Δ, pyp3Δ

Cell Phenotype

Term NameGenotypes
inviable swollen spherical vegetative cellpyp2Δ, pyp1+
vegetative cell lysispyp2Δ, pyp1+
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in atf1 transcription factor, Atf-CREB family Atf1
FYPO affected by mutation in gad8 AGC family protein kinase Gad8
FYPO affected by mutation in mcs4 response regulator Mcs4
FYPO affected by mutation in mip1 WD repeat protein, Raptor homolog Mip1
FYPO affected by mutation in pim1 RCC1 family Ran GDP/GTP exchange factor
FYPO affected by mutation in pmk1 MAP kinase Pmk1
FYPO affected by mutation in pyp2 tyrosine phosphatase Pyp2
FYPO affected by mutation in sty1 MAP kinase Sty1
FYPO affected by mutation in tor1 phosphatidylinositol kinase Tor1
FYPO affected by mutation in win1 MAP kinase kinase kinase Win1
FYPO affected by mutation in wis1 MAP kinase kinase Wis1
FYPO affected by mutation in wis4 MAP kinase kinase kinase Wis4
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons5202366..5205202
5' UTR5202366..5202822SPPYP2
3' UTR5204959..5205202SPC03110
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00102 Pfam IPR000242 PTP type protein phosphatase 460 695 3
PF00581 Pfam IPR001763 Rhodanese-like domain 18 123 6
SM00194 SMART IPR000242 PTP type protein phosphatase 421 700 3
SM00404 SMART IPR003595 Protein-tyrosine phosphatase, catalytic 590 697 6
SM00450 SMART IPR001763 Rhodanese-like domain 11 127 6
PS50055 Prosite Profiles IPR000242 PTP type protein phosphatase 461 698 3
PS50056 Prosite Profiles IPR000387 Tyrosine specific protein phosphatases domain 608 689 6
PS50206 Prosite Profiles IPR001763 Rhodanese-like domain 21 130 7
PS00383 Prosite Patterns IPR016130 Protein-tyrosine phosphatase, active site 628 638 7
PTHR19134 HMMPANTHER 460 707 3
3.40.250.10 Gene3D IPR001763 Rhodanese-like domain 4 130 8
3.90.190.10 Gene3D IPR029021 Protein-tyrosine phosphatase-like 456 700 9
SSF52799 SuperFamily IPR029021 Protein-tyrosine phosphatase-like 454 697 9
SSF52821 SuperFamily IPR001763 Rhodanese-like domain 26 127 7
PR00700 PRINTS IPR000242 PTP type protein phosphatase 485 492 3
PR00700 PRINTS IPR000242 PTP type protein phosphatase 499 519 3
PR00700 PRINTS IPR000242 PTP type protein phosphatase 589 606 3
PR00700 PRINTS IPR000242 PTP type protein phosphatase 625 643 3
PR00700 PRINTS IPR000242 PTP type protein phosphatase 665 680 3
PR00700 PRINTS IPR000242 PTP type protein phosphatase 681 691 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.61 Da
Charge 24.00
Codon Adaptation Index 0.42
Isoelectric point 8.89
Molecular weight 79.36 kDa
Number of residues 711
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1675
present during mitotic M phaseS48
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S48 PMID:21712547
O-phospho-L-threonine 700
present during mitotic M phaseT51
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T51 PMID:21712547
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelpresent during GO:0072690Western blot evidencePMID:17952063
decreased during GO:0072752Western blot evidencePMID:17952063
decreased during GO:0009267Western blot evidencePMID:17952063
RNA levelincreased during GO:0070301Northern assay evidencePMID:8649397
increased during GO:0071472Northern assay evidencePMID:8824588
increased during GO:0071472Northern assay evidencePMID:8824587
increased during GO:0034605Northern assay evidencePMID:8649397
present during GO:0072690Northern assay evidencePMID:18235227
increased during GO:0071472Northern assay evidencePMID:17881729
increased during GO:0034605expression microarray evidencePMID:12529438
present during GO:0072690Northern assay evidencePMID:8649397
increased during GO:0070301Northern assay evidencePMID:9450957
increased during GO:0034599expression microarray evidencePMID:12529438
increased in presence of cadmium sulfate expression microarray evidencePMID:12529438
increased during GO:0034605Northern assay evidencePMID:9450957
increased during GO:0071470expression microarray evidencePMID:12529438
present during GO:0072690Northern assay evidencePMID:1464319
increased during GO:0071472Northern assay evidencePMID:18235227
increased during GO:0072740Northern assay evidencePMID:9450957
absent during GO:0072690Northern assay evidencePMID:9450957
increased in presence of methyl methanesulfonate expression microarray evidencePMID:12529438
increased during GO:0034605Northern assay evidencePMID:8824588
increased during GO:0071470Northern assay evidencePMID:8649397
increased during GO:0072709Northern assay evidencePMID:8824588
increased during GO:0071470expression microarray evidencePMID:12529438
increased during GO:0071472Northern assay evidencePMID:9450957
increased during GO:0070301Northern assay evidencePMID:8824588
increased during GO:0034644Northern assay evidencePMID:9154834
increased during GO:0071472Northern assay evidencePMID:9154834

Quantitative Gene Expression

View graphical display of gene expression data for pyp2 (SPAC19D5.01)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.95during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.14during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3422
conserved in vertebrates3397
conserved in eukaryotes only2504
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load gene that interact physically with SPAC19D5.01 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bysty1MAP kinase Sty1 Affinity Capture-RNAPMID:22144913
affinity capturessty1MAP kinase Sty1 Affinity Capture-WesternPMID:8649397
modifiessty1MAP kinase Sty1 Biochemical ActivityPMID:8649397
modifieswee1M phase inhibitor protein kinase Wee1 Biochemical ActivityPMID:8256510
binds activation domain construct withsty1MAP kinase Sty1 Two-hybridPMID:23695164
Genetic Interactions

Source: BioGRID

Load gene that interact genetically with SPAC19D5.01 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
enhances phenotype ofcdc25M phase inducer tyrosine phosphatase Cdc25 Phenotypic EnhancementPMID:1464319
overexpression lethal tocdr1NIM1 family serine/threonine protein kinase Cdr1/Nim1 Dosage LethalityPMID:7937842
overexpression lethal tocdc25M phase inducer tyrosine phosphatase Cdc25 Dosage LethalityPMID:7937842
rescuestor1phosphatidylinositol kinase Tor1 Phenotypic SuppressionPMID:19417002
rescuescpc2RACK1 ortholog Cpc2 Phenotypic SuppressionPMID:23843946
rescuesubp3ubiquitin C-terminal hydrolase Ubp3 Phenotypic SuppressionPMID:19547744
rescuespka1cAMP-dependent protein kinase catalytic subunit Pka1 Phenotypic SuppressionPMID:8832414
rescuescyr1adenylate cyclase Phenotypic SuppressionPMID:8832414
synthetic lethal withpyp1tyrosine phosphatase Pyp1 Synthetic LethalityPMID:7657164
negative genetic interaction withacp1F-actin capping protein alpha subunit Negative GeneticPMID:22681890
negative genetic interaction withSPBC2F12.05csterol binding ankyrin repeat protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPAC17H9.08mitochondrial coenzyme A transmembrane transporter (predicted) Negative GeneticPMID:22681890
negative genetic interaction withwtf21wtf element Wtf21 Negative GeneticPMID:22681890
negative genetic interaction withSPAC18B11.02ctRNA pseudouridine synthase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withpac10prefoldin subunit 3 Pac10 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withapm4AP-2 adaptor complex subunit Apm4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPBC3F6.01cserine/threonine protein phosphatase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withgit5heterotrimeric G protein beta subunit Git5 Negative GeneticPMID:22681890
negative genetic interaction withvps38phophatidylinositol 3-kinase complex subunit Vps38 Negative GeneticPMID:22681890
negative genetic interaction withgmh4alpha-1,2-galactosyltransferase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmug147Schizosaccharomyces specific protein Mug147 Negative GeneticPMID:22681890
negative genetic interaction withSPCP1E11.10ankyrin repeat protein, unknown biological role Negative GeneticPMID:22681890
negative genetic interaction withSPBC32F12.12cGolgi membrane protein involved in vesicle-mediated transport (predicted) Negative GeneticPMID:22681890
negative genetic interaction withSPCC594.01DUF1769 family protein Negative GeneticPMID:22681890
negative genetic interaction withdbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
negative genetic interaction withdli1meiotic dynein intermediate light chain Dli1/Dil1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC18G6.12cThiJ domain protein (predicted) Negative GeneticPMID:22681890
negative genetic interaction withhmt2sulfide-quinone oxidoreductase Negative GeneticPMID:22681890
negative genetic interaction withmso1endocytic docking protein Mso1 Negative GeneticPMID:22681890
negative genetic interaction withgld1mitochondrial glycerol dehydrogenase Gld1 Negative GeneticPMID:22681890
negative genetic interaction withrps340S ribosomal protein S3 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdsc2Golgi Dsc E3 ligase complex subunit Dsc2 Negative GeneticPMID:21504829
negative genetic interaction withuds1septation protein Uds1 Negative GeneticPMID:22681890
negative genetic interaction withckb2CK2 family regulatory subunit Ckb2 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withmug51variant protein kinase 19 family protein Negative GeneticPMID:22681890
negative genetic interaction withucp12ATP-dependent RNA helicase Ucp12 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withswc2Swr1 complex complex subunit Swc2 Negative GeneticPMID:22681890
negative genetic interaction withrod1arrestin/PY protein involved in ubiquitin-mediated endocytosis Rod1 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withSPCC1281.04pyridoxal reductase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withrps40340S ribosomal protein S4 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withprz1calcineurin responsive transcription factor Prz1 Negative GeneticPMID:22681890
negative genetic interaction withSPAC1805.14Schizosaccharomyces specific protein Negative GeneticPMID:22681890
negative genetic interaction withpvg2galactose residue biosynthesis protein Pvg2 Negative GeneticPMID:22681890
negative genetic interaction withuge1UDP-glucose 4-epimerase Uge1 Negative GeneticPMID:22681890
negative genetic interaction withtrm13tRNA 2'-O-methyltransferase Trm13 (predicted) Negative GeneticPMID:22681890
synthetic growth defect withvps17retromer complex subunit Vps17 Synthetic Growth DefectPMID:18931302
synthetic growth defect withryh1GTPase Ryh1 Synthetic Growth DefectPMID:18931302
synthetic growth defect withslm9hira protein Slm9 Synthetic Growth DefectPMID:18931302
rescued bysty1MAP kinase Sty1 Phenotypic SuppressionPMID:7501024
rescued bycdc2cyclin-dependent protein kinase Cdk1/Cdc2 Phenotypic SuppressionPMID:1448087
rescued bywee1M phase inhibitor protein kinase Wee1 Phenotypic SuppressionPMID:1448087
synthetically rescuescdc25M phase inducer tyrosine phosphatase Cdc25 Synthetic RescuePMID:1464319
positive genetic interaction withSPAC6B12.07cubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withmto1MT organizer Mto1 Positive GeneticPMID:22681890
positive genetic interaction withcsn3COP9/signalosome complex subunit Csn3 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withrfp2SUMO-targeted ubiquitin-protein ligase subunit Rfp2 Positive GeneticPMID:22681890
positive genetic interaction withspa1ornithine decarboxylase antizyme with +1 programmed ribosomal frameshift Spa1 Positive GeneticPMID:22681890
positive genetic interaction withmoc3transcription factor Moc3 Positive GeneticPMID:22681890
positive genetic interaction withzwf2glucose-6-phosphate 1-dehydrogenase Zwf2 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withscs7sphingosine hydroxylase Scs7 Positive GeneticPMID:22681890
overexpression rescuesbgs11,3-beta-glucan synthase catalytic subunit Bgs1 Dosage RescuePMID:23271606
overexpression rescuesmed14mediator complex subunit Med14 Dosage RescuePMID:23271606
overexpression rescuesryh1GTPase Ryh1 Dosage RescuePMID:23271606
External References
Database Identifier Description
NBRP SPAC19D5.01 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC19D5.01 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC19D5.01 BioGRID Interaction Datasets
Expression Viewer SPAC19D5.01 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC19D5.01 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC19D5.01 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC19D5.01 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC19D5.01 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC19D5.01 Transcriptome Viewer (Bähler Lab)
GEO SPAC19D5.01 GEO profiles
PInt SPAC19D5.01 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC19D5.01 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC19D5.01 Fission yeast phenotypic data & analysis
Cyclebase SPAC19D5.01.1 Cell Cycle Data
IntEnz3.1.3.48Integrated relational Enzyme database
Rhea3.1.3.48Annotated reactions database
SPD / RIKEN37/37E03Orfeome Localization Data
UniProtKB/SwissProtP32586Tyrosine-protein phosphatase 2
ModBaseP32586Database of comparative protein structure models
STRINGP32586Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594899tyrosine phosphatase Pyp2
RefSeq mRNANM_001020328972h- tyrosine phosphatase Pyp2 (pyp2), mRNA
European Nucleotide ArchiveAB027789ENA EMBL mapping
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveS51320ENA EMBL mapping
European Nucleotide ArchiveX59599ENA EMBL mapping
European Nucleotide ArchiveAAB24544ENA Protein Mapping
European Nucleotide ArchiveBAA87093ENA Protein Mapping
European Nucleotide ArchiveCAA42167ENA Protein Mapping
European Nucleotide ArchiveCAB16711ENA Protein Mapping
UniParcUPI0000132BF8UniProt Archive

Literature for pyp2

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016