uga1 (SPAC19D5.07)


Gene Standard Nameuga1 Characterisation Statuspublished
Systematic IDSPAC19D5.07 Feature Typeprotein coding
Synonyms Name Description
Product4-aminobutyrate aminotransferase (GABA transaminase) Product Size474aa, 52.95 kDa
Genomic Location Chromosome I, 5222447-5224795 (2349nt); CDS:5223184-5224608 (1425nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
4-aminobutyrate transaminase activity1
Annotation ExtensionEvidenceWith/FromReference
pyridoxal phosphate binding25
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
gamma-aminobutyric acid catabolic process3
Annotation ExtensionEvidenceWith/FromReference
glutamate metabolic process24
Annotation ExtensionEvidenceWith/FromReference
nitrogen utilization5
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
mitochondrion761
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
viable vegetative cell populationuga1ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
viable vegetative cell with normal cell morphologyuga1ΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons5222447..5224795
mRNA5222447..5224795
5' UTR5222447..5223183PMID:21511999
CDS5223184..5224608
3' UTR5224609..5224795PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00202 Pfam IPR005814 Aminotransferase class-III 55 423 7
PS00600 Prosite Patterns IPR005814 Aminotransferase class-III 299 338 5
PTHR11986 HMMPANTHER IPR005814 Aminotransferase class-III 1 474 7
PTHR11986:SF6 HMMPANTHER IPR004631 4-aminobutyrate aminotransferase, eukaryotic 1 474 1
3.90.1150.10 Gene3D IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 46 87 25
3.40.640.10 Gene3D IPR015421 Pyridoxal phosphate-dependent transferase, major region, subdomain 1 88 377 31
3.90.1150.10 Gene3D IPR015422 Pyridoxal phosphate-dependent transferase, major region, subdomain 2 380 474 25
SSF53383 SuperFamily IPR015424 Pyridoxal phosphate-dependent transferase 38 473 31
PIRSF000521 PIRSF IPR005814 Aminotransferase class-III 1 474 1
TIGR00699 tigrfam IPR004631 4-aminobutyrate aminotransferase, eukaryotic 12 474 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 111.70 Da
Charge 6.00
Isoelectric point 7.07
Molecular weight 52.95 kDa
Number of residues 474
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5539during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4978during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
2864during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
679.79during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
3390.7during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
1.6during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
3.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in bacteria1001
conserved in metazoa3424
conserved in vertebrates3399
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
positive genetic interaction withccr4CCR4-Not complex subunit Ccr4 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withfft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
negative genetic interaction withhrr1Helicase Required for RNAi-mediated heterochromatin assembly Hrr1 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC19D5.07 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC19D5.07 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC19D5.07 BioGRID Interaction Datasets
Expression Viewer SPAC19D5.07 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC19D5.07 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC19D5.07 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC19D5.07 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC19D5.07 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC19D5.07 Transcriptome Viewer (Bähler Lab)
GEO SPAC19D5.07 GEO profiles
PInt SPAC19D5.07 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC19D5.07 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC19D5.07 Fission yeast phenotypic data & analysis
Cyclebase SPAC19D5.07.1 Cell Cycle Data
IntEnz2.6.1.19Integrated relational Enzyme database
Rhea2.6.1.19Annotated reactions database
SPD / RIKEN21/21H08Orfeome Localization Data
UniProtKB/SwissProtO138374-aminobutyrate aminotransferase
ModBaseO13837Database of comparative protein structure models
STRINGO13837Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_5949054-aminobutyrate aminotransferase (GABA transaminase)
RefSeq mRNANM_001020336972h- 4-aminobutyrate aminotransferase (GABA transaminase) (uga1), mRNA
European Nucleotide ArchiveCAB16717.1ENA Protein Mapping
MetaCycPWY-4321Glutamate degradation IV
MetaCycPWY-50224-aminobutyrate degradation V
MetaCycPWY-65354-aminobutyrate degradation I
MetaCycPWY-65364-aminobutyrate degradation III
MetaCycPWY-65374-aminobutyrate degradation II
KEGG_Enzyme00250+2.6.1.19Alanine, aspartate and glutamate metabolism
KEGG_Enzyme00410+2.6.1.19beta-Alanine metabolism
KEGG_Enzyme00640+2.6.1.19Propanoate metabolism
KEGG_Enzyme00650+2.6.1.19Butanoate metabolism
UniParcUPI000012B12BUniProt Archive

Literature for uga1

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015