vht1 (SPAC1B3.16c)


Gene Standard Namevht1 Characterisation Statuspublished
Systematic IDSPAC1B3.16c Feature Typeprotein coding
Synonyms Name DescriptionVitamin H Transporter
Productvitamin H transmembrane transporter Vht1 Product Size568aa, 62.82 kDa
Genomic Location Chromosome I, 4961033-4958698 (2336nt); CDS:4960800-4959094 (1707nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
biotin transmembrane transporter activity1
Annotation ExtensionEvidenceWith/FromReference
dethiobiotin transmembrane transporter activity1
Annotation ExtensionEvidenceWith/FromReference
solute:proton symporter activity6
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
biotin import into cell1
Annotation ExtensionEvidenceWith/FromReference
dethiobiotin import into cell1
Annotation ExtensionEvidenceWith/FromReference
transmembrane transport337
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Golgi apparatus358
Annotation ExtensionEvidenceWith/FromReference
integral component of plasma membrane53
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viability depends on conditions

Population Phenotype

Term NameGenotypesCount
inviable vegetative cell populationvht1Δ1450
sensitive to bortezomibvht1Δ255
sensitive to hydroxyureavht1Δ547
sensitive to thiabendazolevht1Δ254
viable vegetative cell populationvht1Δ3831

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cyclevht1Δ849
decreased biotin importvht1Δ1
elongated vegetative cellvht1Δ787
increased biotin importvht1+1
Target Of
OntologyRelationshipGeneProduct
GO substrate of sst2 human AMSH/STAMBP protein homolog, ubiquitin specific-protease
GO substrate of ubp15 ubiquitin C-terminal hydrolase Ubp15
GO substrate of ubp4 ubiquitin C-terminal hydrolase Ubp4
GO substrate of ubp5 ubiquitin C-terminal hydrolase Ubp5
GO substrate of ubp9 ubiquitin C-terminal hydrolase Ubp9
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4961033..4958698
5' UTR4961033..4960801PMID:21511999
3' UTR4959093..4958698PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07690 Pfam IPR011701 Major facilitator superfamily 89 485 50
TMhelix TMHMM 86 108 960
TMhelix TMHMM 123 145 960
TMhelix TMHMM 158 177 960
TMhelix TMHMM 187 209 960
TMhelix TMHMM 222 244 960
TMhelix TMHMM 259 281 960
TMhelix TMHMM 345 367 960
TMhelix TMHMM 382 404 960
TMhelix TMHMM 411 428 960
TMhelix TMHMM 438 460 960
TMhelix TMHMM 473 495 960
TMhelix TMHMM 510 528 960
PTHR11662 HMMPANTHER 6 38 13
PTHR11662 HMMPANTHER 61 285 13
PTHR11662 HMMPANTHER 309 560 13
PTHR11662:SF2 HMMPANTHER 6 38 2
PTHR11662:SF2 HMMPANTHER 61 285 2
PTHR11662:SF2 HMMPANTHER 309 560 2
1.20.1250.20 Gene3D Glycerate/sugar phosphate transporter, conserved site 80 285 72
1.20.1250.20 Gene3D Glycerate/sugar phosphate transporter, conserved site 344 532 72
SSF103473 SuperFamily IPR020846 Major facilitator superfamily domain 77 284 76
SSF103473 SuperFamily IPR020846 Major facilitator superfamily domain 323 524 76

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.60 Da
Charge 8.00
Codon Adaptation Index 0.50
Isoelectric point 8.04
Molecular weight 62.82 kDa
Number of residues 568
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1675
present during mitotic M phaseS32
level fluctuates during mitotic cell cycleS35
present during mitotic M phaseS12
present during mitotic M phaseS47
present during mitotic M phaseS35
present during mitotic M phaseS30
present during mitotic M phaseS13
present during mitotic M phaseS9
present during mitotic M phaseS28
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S9 PMID:21712547
present during mitotic M phase experimental evidence S12 PMID:21712547
present during mitotic M phase experimental evidence S13 PMID:21712547
present during mitotic M phase experimental evidence S28 PMID:21712547
present during mitotic M phase experimental evidence S30 PMID:21712547
present during mitotic M phase experimental evidence S32 PMID:21712547
present during mitotic M phase experimental evidence S35 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S35 PMID:24763107
present during mitotic M phase experimental evidence S47 PMID:21712547
O-phospho-L-threonine 700
present during mitotic M phaseT8
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T8 PMID:21712547
ubiquitinylated lysineK315, K329 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K315 PMID:26412298
mass spectrometry evidence K329 PMID:26412298
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:1990383Northern assay evidencePMID:12557275

Quantitative Gene Expression

View graphical display of gene expression data for vht1 (SPAC1B3.16c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
94553during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
102734during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
102038during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
86706during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4145.35during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
83768during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complements S. cerevisiae VHT1full PMID:12557275
Genome Organisation
DescriptionQualifierReferenceCount
tandem duplication30
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4604
conserved in eukaryotes4514
conserved in bacteria1000
conserved in archaea238
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


External References
Database Identifier Description
NBRP SPAC1B3.16c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1B3.16c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1B3.16c BioGRID Interaction Datasets
Expression Viewer SPAC1B3.16c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1B3.16c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1B3.16c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1B3.16c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1B3.16c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1B3.16c Transcriptome Viewer (Bähler Lab)
GEO SPAC1B3.16c GEO profiles
PInt SPAC1B3.16c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1B3.16c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1B3.16c Fission yeast phenotypic data & analysis
Cyclebase SPAC1B3.16c.1 Cell Cycle Data
SPD / RIKEN35/35E01Orfeome Localization Data
UniProtKB/SwissProtO13880Vitamin H transporter 1
ModBaseO13880Database of comparative protein structure models
STRINGO13880Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594801vitamin H transporter Vth1
RefSeq mRNANM_001020229972h- vitamin H transporter Vth1 (vht1), mRNA
European Nucleotide ArchiveAY179181ENA EMBL mapping
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveAAO22560ENA Protein Mapping
European Nucleotide ArchiveCAB11242ENA Protein Mapping
UniParcUPI0000005B67UniProt Archive

Literature for vht1

Search: Europe PMC or PubMed

Release Version: PomBase:30_57 - 27 Jan 2016