vht1 (SPAC1B3.16c)


Gene Standard Namevht1 Characterisation Statuspublished
Systematic IDSPAC1B3.16c Feature Typeprotein coding
Synonyms Name DescriptionVitamin H Transporter
Productvitamin H transporter Vht1 Product Size568aa, 62.82 kDa
Genomic Location Chromosome I, 4961033-4958698 (2336nt); CDS:4960800-4959094 (1707nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:1901603biotin transmembrane transporter activityIMPPMID:125572751
GO:1901604dethiobiotin transmembrane transporter activityIMPPMID:125572751
GO:0015295solute:proton symporter activityIMPPMID:125572756
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0044756biotin import into cellIMPPMID:125572751
GO:0044757dethiobiotin import into cellIMPPMID:125572751
GO:0034220ion transmembrane transportIMPPMID:12557275189
GO:0015992proton transportIMPPMID:1255727550
GO:0035461vitamin transmembrane transportIMPPMID:125572757
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005794Golgi apparatusIDAPMID:16823372355
GO:0005887integral component of plasma membraneIMPPMID:1255727549
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationCell growth assayvht1ΔNullPECO:0000005, PECO:0000102, PECO:0000194, PECO:0000126PMID:125572751315
FYPO:0001701sensitive to bortezomib256
expressivity FYPO_EXT:0000001Cell growth assayvht1ΔNullPECO:0000005, PECO:0000137PMID:21760946
FYPO:0000088sensitive to hydroxyureaCell growth assayvht1ΔNullPECO:0000137, PECO:0000005PMID:23173672506
FYPO:0000091sensitive to thiabendazoleCell growth assayvht1ΔNullPECO:0000137, PECO:0000005PMID:23173672164
FYPO:0002060viable vegetative cell populationMicroscopyvht1ΔNullPMID:204732893760
Microscopyvht1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopyvht1ΔNullPECO:0000005, PECO:0000137PMID:23697806624
FYPO:0003388decreased biotin importSubstance quantification evidencevht1ΔNullPMID:125572751
FYPO:0001122elongated vegetative cell649
penetrance FYPO_EXT:0000003Microscopyvht1ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0001745increased biotin importSubstance quantification evidencevht1+ (wild type)OverexpressionPMID:125572751
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
149610334958698

UTRs

Region Coordinates Reference
five_prime_UTR4961033..4960801PMID:21511999
three_prime_UTR4959093..4958698PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07690 Pfam IPR011701 Major facilitator superfamily 89 483 50
TMhelix TMHMM 473 495 959
TMhelix TMHMM 382 404 959
TMhelix TMHMM 187 209 959
TMhelix TMHMM 86 108 959
TMhelix TMHMM 411 428 959
TMhelix TMHMM 259 281 959
TMhelix TMHMM 158 177 959
TMhelix TMHMM 438 460 959
TMhelix TMHMM 510 528 959
TMhelix TMHMM 222 244 959
TMhelix TMHMM 123 145 959
TMhelix TMHMM 345 367 959
PTHR11662 HMMPANTHER 8 558 13
PTHR11662:SF2 HMMPANTHER 8 558 2
1.20.1250.20 Gene3D 80 285 69
1.20.1250.20 Gene3D 344 532 69
SSF103473 SuperFamily IPR016196 Major facilitator superfamily domain, general substrate transporter 77 284 76
SSF103473 SuperFamily IPR016196 Major facilitator superfamily domain, general substrate transporter 323 524 76

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.60 Da
Charge 8.00
Isoelectric point 8.04
Molecular weight 62.82 kDa
Number of residues 568
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineS28PMID:217125471663
present during mitotic M phase
present during mitotic M phaseS13PMID:21712547
present during mitotic M phaseS9PMID:21712547
present during mitotic M phaseS30PMID:21712547
level fluctuates during mitotic cell cycleS35PMID:24763107
present during mitotic M phaseS47PMID:21712547
present during mitotic M phaseS32PMID:21712547
present during mitotic M phaseS35PMID:21712547
present during mitotic M phaseS12PMID:21712547
MOD:00047O-phospho-L-threonineT8PMID:21712547682
present during mitotic M phase
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:1990383Northern assay evidencePMID:12557275

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
83768during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
4145.35during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
94553during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
102734during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
102038during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
86706during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
15during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complements S. cerevisiae VHT1full PMID:12557275
Genome Organisation
DescriptionQualifierReferenceCount
tandem duplication31
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in bacteria1001
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
hip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
pof13F-box protein Pof13 Negative GeneticPMID:22681890
dad1DASH complex subunit Dad1 Negative GeneticPMID:22681890
set1histone lysine methyltransferase Set1 Positive GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
SPCC1223.01ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
rik1silencing protein Rik1 Positive GeneticPMID:22681890
SPAC2F3.16ubiquitin-protein ligase E3 (predicted) Negative GeneticPMID:22681890
lsk1P-TEFb-associated cyclin-dependent protein kinase Lsk1 Positive GeneticPMID:22681890
spc34DASH complex subunit Spc34 Negative GeneticPMID:22681890
dak1dihydroxyacetone kinase Dak1 Negative GeneticPMID:22681890
mid1medial ring protein Mid1 Positive GeneticPMID:22681890
rok1ATP-dependent RNA helicase Rok1 (predicted) Negative GeneticPMID:22681890
lub1WD repeat protein Lub1 Negative GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
dbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
atg6beclin family protein, involved in autophagy Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
dad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
nup40nucleoporin Nup40 Negative GeneticPMID:22681890
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
SPBC29A3.09cAAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
msa1RNA-binding protein Msa1 Positive GeneticPMID:22681890
dad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
gcn2eIF2 alpha kinase Gcn2 Negative GeneticPMID:22681890
mug154conserved fungal protein Negative GeneticPMID:22681890
SPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
SPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:22681890
pmc2mediator complex subunit Pmc2/Med1 Negative GeneticPMID:22681890
atp11F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:22681890
pob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
pst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
cph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
sdc1Dpy-30 domain protein Sdc1 Negative GeneticPMID:22681890
csn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
tsc1hamartin Negative GeneticPMID:22681890
amk2AMP-activated protein kinase beta subunit Amk2 Positive GeneticPMID:22681890
reb1RNA polymerase I transcription termination factor Reb1 Negative GeneticPMID:22681890
ssp2serine/threonine protein kinase Ssp2 Positive GeneticPMID:22681890
php3CCAAT-binding factor complex subunit Php3 Negative GeneticPMID:22681890
srb11cyclin CycC, Srb mediator subunit Srb11 Positive GeneticPMID:22681890
sts5RNB-like protein Negative GeneticPMID:22681890
SPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
bun107WD repeat protein, human WDR48 family Bun107 Negative GeneticPMID:22681890
ssu72phosphoric ester hydrolase Ssu72 (predicted) Negative GeneticPMID:22681890
mkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
ubp2ubiquitin C-terminal hydrolase Ubp2 Positive GeneticPMID:22681890
ace2transcription factor Ace2 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
knd1Cullin-associated NEDD8-dissociated protein Knd1 (predicted) Positive GeneticPMID:22681890
dam1DASH complex subunit Dam1 Negative GeneticPMID:22681890
tsc2tuberin Negative GeneticPMID:22681890
csn2COP9/signalosome complex subunit Csn2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC1B3.16c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1B3.16c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1B3.16c BioGRID Interaction Datasets
Expression Viewer SPAC1B3.16c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1B3.16c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1B3.16c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1B3.16c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1B3.16c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1B3.16c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1B3.16c Cell Cycle Data
GEO SPAC1B3.16c GEO profiles
PInt SPAC1B3.16c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2542518vitamin H transporter Vth1
EntrezGene2542518vitamin H transporter Vth1
SPD / RIKEN35/35E01Orfeome Localization Data
UniProtKB/SwissProtO13880Vitamin H transporter 1
ModBaseO13880Database of comparative protein structure models
STRINGO13880Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594801vitamin H transporter Vth1
RefSeq mRNANM_001020229972h- vitamin H transporter Vth1 (vht1), mRNA
European Nucleotide ArchiveAY179181ENA EMBL mapping
European Nucleotide ArchiveAAO22560ENA Protein Mapping
European Nucleotide ArchiveCAB11242ENA Protein Mapping
European Nucleotide ArchiveCAB11242.1ENA Protein Mapping
UniParcUPI0000005B67UniProt Archive

Literature for vht1

Search: Europe PMC or PubMed

Release Version: PomBase:22_44 - 08 Jul 2014