vht1 (SPAC1B3.16c)

Gene Standard Namevht1 Characterisation Statuspublished
Systematic IDSPAC1B3.16c Feature Typeprotein coding
Synonyms Name DescriptionVitamin H Transporter
Productvitamin H transmembrane transporter Vht1 Product Size568aa, 62.82 kDa
Genomic Location Chromosome I, 4961033-4958698 (2336nt); CDS:4960800-4959094 (1707nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
biotin transmembrane transporter activity1
Annotation ExtensionEvidenceWith/FromReference
dethiobiotin transmembrane transporter activity1
Annotation ExtensionEvidenceWith/FromReference
solute:proton symporter activity6
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
biotin import into cell1
Annotation ExtensionEvidenceWith/FromReference
cation transmembrane transport130
Annotation ExtensionEvidenceWith/FromReference
dethiobiotin import into cell1
Annotation ExtensionEvidenceWith/FromReference
proton transport50
Annotation ExtensionEvidenceWith/FromReference
vitamin transmembrane transport6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Golgi apparatus357
Annotation ExtensionEvidenceWith/FromReference
integral component of plasma membrane49
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viability depends on conditions

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationvht1ΔNull1427
sensitive to bortezomib256
expressivity FYPO_EXT:0000001vht1ΔNull
sensitive to hydroxyureavht1ΔNull518
sensitive to thiabendazolevht1ΔNull185
viable vegetative cell populationvht1ΔNull3781

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclevht1ΔNull794
decreased biotin importvht1ΔNull1
elongated vegetative cell703
penetrance FYPO_EXT:0000003vht1ΔNull
increased biotin importvht1+ (wild type)Overexpression1
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF07690 Pfam IPR011701 Major facilitator superfamily 89 483 50
TMhelix TMHMM 86 108 959
TMhelix TMHMM 158 177 959
TMhelix TMHMM 187 209 959
TMhelix TMHMM 259 281 959
TMhelix TMHMM 345 367 959
TMhelix TMHMM 382 404 959
TMhelix TMHMM 411 428 959
TMhelix TMHMM 510 528 959
TMhelix TMHMM 123 145 959
TMhelix TMHMM 473 495 959
TMhelix TMHMM 438 460 959
TMhelix TMHMM 222 244 959
PTHR11662 HMMPANTHER 8 558 13
PTHR11662:SF2 HMMPANTHER 8 558 2
1.20.1250.20 Gene3D 344 532 72
1.20.1250.20 Gene3D 80 285 72
SSF103473 SuperFamily IPR020846 Major facilitator superfamily domain 323 524 76
SSF103473 SuperFamily IPR020846 Major facilitator superfamily domain 77 284 76

View domain organization at Pfam

Protein Properties

Ave. residue weight 110.60 Da
Charge 8.00
Isoelectric point 8.04
Molecular weight 62.82 kDa
Number of residues 568

Protein Modifications

Term NameResidueCount
O-phospho-L-serine 1670
present during mitotic M phaseS35
present during mitotic M phaseS9
present during mitotic M phaseS12
present during mitotic M phaseS30
level fluctuates during mitotic cell cycleS35
present during mitotic M phaseS28
present during mitotic M phaseS32
present during mitotic M phaseS13
present during mitotic M phaseS47
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence S9 PMID:21712547
present during mitotic M phase experimental evidence S12 PMID:21712547
present during mitotic M phase experimental evidence S13 PMID:21712547
present during mitotic M phase experimental evidence S28 PMID:21712547
present during mitotic M phase experimental evidence S30 PMID:21712547
present during mitotic M phase experimental evidence S32 PMID:21712547
level fluctuates during mitotic cell cycle experimental evidence S35 PMID:24763107
present during mitotic M phase experimental evidence S35 PMID:21712547
present during mitotic M phase experimental evidence S47 PMID:21712547
O-phospho-L-threonine 693
present during mitotic M phaseT8
Annotation ExtensionEvidenceResidueReference
present during mitotic M phase experimental evidence T8 PMID:21712547
Gene Expression

Qualitative Gene Expression

RNA levelincreased during GO:1990383Northern assay evidencePMID:12557275

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
94553during GO:0000080PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
102734during GO:0000084PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
102038during GO:0000085PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
86706during GO:0000087PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
83768during GO:0072690PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
4145.35during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
15during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
0.78during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
functionally complements S. cerevisiae VHT1full PMID:12557275
Genome Organisation
tandem duplication30
Species Distribution
conserved in fungi4604
conserved in eukaryotes4516
conserved in bacteria1000
conserved in archaea237

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
vps35retromer complex subunit Vps35 Negative GeneticPMID:22681890
cdt2WD repeat protein Cdt2 Negative GeneticPMID:22681890
hip1hira protein, histone chaperone Hip1 Negative GeneticPMID:22681890
pof13F-box protein Pof13 Negative GeneticPMID:22681890
dad1DASH complex subunit Dad1 Negative GeneticPMID:22681890
set1histone lysine methyltransferase Set1 Positive GeneticPMID:22681890
php5CCAAT-binding factor complex subunit Php5 Negative GeneticPMID:22681890
SPCC1223.01ubiquitin-protein ligase E3 (predicted) Positive GeneticPMID:22681890
rik1silencing protein Rik1 Positive GeneticPMID:22681890
SPAC2F3.16ubiquitin-protein ligase E3 (predicted) Negative GeneticPMID:22681890
lsk1P-TEFb-associated cyclin-dependent protein kinase Lsk1 Positive GeneticPMID:22681890
spc34DASH complex subunit Spc34 Negative GeneticPMID:22681890
xap5xap-5-like protein Negative GeneticPMID:24957674
dak1dihydroxyacetone kinase Dak1 Negative GeneticPMID:22681890
mid1medial ring protein Mid1 Positive GeneticPMID:22681890
rok1ATP-dependent RNA helicase Rok1 (predicted) Negative GeneticPMID:22681890
lub1WD repeat protein Lub1 Negative GeneticPMID:22681890
pef1Pho85/PhoA-like cyclin-dependent kinase Pef1 Negative GeneticPMID:22681890
dbr1RNA lariat debranching enzyme Dbr1 Negative GeneticPMID:22681890
ddb1damaged DNA binding protein Ddb1 Negative GeneticPMID:22681890
vph2endoplasmic reticulum membrane protein involved in assembly of the V-ATPase (predicted) Negative GeneticPMID:22681890
atg6beclin family protein, involved in autophagy Positive GeneticPMID:22681890
ask1DASH complex subunit Ask1 Negative GeneticPMID:22681890
dad4DASH complex subunit Dad4 Negative GeneticPMID:22681890
nup40nucleoporin Nup40 Negative GeneticPMID:22681890
alp13MRG family Clr6 histone deacetylase complex subunit Alp13 Negative GeneticPMID:22681890
gcn20AAA family ATPase Gcn20 (predicted) Negative GeneticPMID:22681890
msa1RNA-binding protein Msa1 Positive GeneticPMID:22681890
dad2DASH complex subunit Dad2 Negative GeneticPMID:22681890
cph1Clr6 histone deacetylase associated PHD protein-1 Cph1 Negative GeneticPMID:22681890
gcn2eIF2 alpha kinase Gcn2 Negative GeneticPMID:22681890
mug154conserved fungal protein Negative GeneticPMID:22681890
SPBC31F10.10czf-MYND type zinc finger protein Negative GeneticPMID:22681890
kin1microtubule affinity-regulating kinase Kin1 Negative GeneticPMID:22681890
SPBC56F2.08cRNA-binding protein (predicted) Negative GeneticPMID:22681890
pmc2mediator complex subunit Pmc2/Med1 Negative GeneticPMID:22681890
atp11mitochondrial F1-ATPase chaperone Atp11 (predicted) Negative GeneticPMID:22681890
pob3FACT complex subunit Pob3 Negative GeneticPMID:22681890
pst2Clr6 histone deacetylase complex subunit Pst2 Negative GeneticPMID:22681890
ccr4CCR4-Not complex subunit Ccr4 (predicted) Negative GeneticPMID:22681890
arp42SWI/SNF and RSC complex subunit Arp42 Negative GeneticPMID:22681890
rpl160160S ribosomal protein L13/L16 (predicted) Negative GeneticPMID:22681890
cph2Clr6 histone deacetylase associated PHD protein-2 Cph2 Negative GeneticPMID:22681890
sdc1Dpy-30 domain protein Sdc1 Negative GeneticPMID:22681890
csn1COP9/signalosome complex subunit Csn1 Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
tsc1hamartin Negative GeneticPMID:22681890
amk2AMP-activated protein kinase beta subunit Amk2 Positive GeneticPMID:22681890
reb1RNA polymerase I transcription termination factor/ RNA polymerase II transcription factor Reb1 Negative GeneticPMID:22681890
ssp2serine/threonine protein kinase Ssp2 Positive GeneticPMID:22681890
php3CCAAT-binding factor complex subunit Php3 Negative GeneticPMID:22681890
srb11cyclin CycC, Srb mediator subunit Srb11 Positive GeneticPMID:22681890
sts5RNB-like protein Negative GeneticPMID:22681890
SPCC162.11curidine kinase/uracil phosphoribosyltransferase (predicted) Positive GeneticPMID:22681890
bun107WD repeat protein, human WDR48 family Bun107 Negative GeneticPMID:22681890
ssu72phosphoric ester hydrolase Ssu72 (predicted) Negative GeneticPMID:22681890
mkh1MEK kinase (MEKK) Mkh1 Negative GeneticPMID:22681890
ubp2ubiquitin C-terminal hydrolase Ubp2 Positive GeneticPMID:22681890
ace2transcription factor Ace2 Negative GeneticPMID:22681890
fft3SMARCAD1 family ATP-dependent DNA helicase Fft3 Positive GeneticPMID:22681890
knd1Cullin-associated NEDD8-dissociated protein Knd1 (predicted) Positive GeneticPMID:22681890
dam1DASH complex subunit Dam1 Negative GeneticPMID:22681890
tsc2tuberin Negative GeneticPMID:22681890
csn2COP9/signalosome complex subunit Csn2 Negative GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC1B3.16c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1B3.16c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1B3.16c BioGRID Interaction Datasets
Expression Viewer SPAC1B3.16c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1B3.16c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1B3.16c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1B3.16c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1B3.16c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1B3.16c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1B3.16c Cell Cycle Data
GEO SPAC1B3.16c GEO profiles
PInt SPAC1B3.16c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1B3.16c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1B3.16c Fission yeast phenotypic data & analysis
SPD / RIKEN35/35E01Orfeome Localization Data
UniProtKB/SwissProtO13880Vitamin H transporter 1
ModBaseO13880Database of comparative protein structure models
STRINGO13880Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594801vitamin H transporter Vth1
RefSeq mRNANM_001020229972h- vitamin H transporter Vth1 (vht1), mRNA
European Nucleotide ArchiveAY179181ENA EMBL mapping
European Nucleotide ArchiveCAB11242.1ENA Protein Mapping
UniParcUPI0000005B67UniProt Archive

Literature for vht1

Search: Europe PMC or PubMed

Release Version: PomBase:25_48 - 10 Jan 2015