lkh1 (SPAC1D4.11c)


Gene Standard Namelkh1 Characterisation Statuspublished
Systematic IDSPAC1D4.11c Feature Typeprotein coding
Synonymskic1 Name Description
Productdual specificity protein kinase Lkh1 Product Size690aa, 77.49 kDa
Genomic Location Chromosome I, 660381-656076 (4306nt); CDS:658832-656760 (2073nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037557
GO:0004674protein serine/threonine kinase activity112
has substrate rum1IDAPMID:23376070
GO:0004712protein serine/threonine/tyrosine kinase activityIEA EC:2.7.12.1GO_REF:00000036
GO:0004713protein tyrosine kinase activityIEAUniProtKB-KW:KW-0829GO_REF:00000375
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0034599cellular response to oxidative stressIMPPMID:1462329262
GO:0035556intracellular signal transduction148
regulates atf1TASPMID:14623292
GO:0031658negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycleIMPPMID:233760701
GO:0060258negative regulation of filamentous growthIMPPMID:117413261
GO:0060257negative regulation of flocculationIMPPMID:117413261
GO:0018107peptidyl-threonine phosphorylationIDAPMID:233760706
GO:0048026positive regulation of mRNA splicing, via spliceosomeIMPPMID:217454682
GO:0060237regulation of fungal-type cell wall organizationIMPPMID:125658233
GO:1902468regulation of mitotic barrier septum assemblyIMPPMID:1256582313
GO:1902467regulation of mitotic cytokinetic cell separationIMPPMID:125658237
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005634nucleusIDAPMID:168233722740
dependent on lkh1IMPPMID:21745468
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002296cone-shaped colony morphologyMicroscopylkh1ΔNullPECO:0000005, PECO:0000137PMID:236978061
FYPO:0000709decreased cell population growth during nitrogen starvationCell growth assaylkh1ΔNullPECO:0000127, PECO:0000103, PECO:0000126PMID:2337607035
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaylkh1ΔNullPECO:0000127, PECO:0000102, PECO:0000126PMID:23376070106
Cell growth assaylkh1ΔNullPECO:0000137, PECO:0000102PMID:23376070
FYPO:0000155increased flocculationCell growth assaylkh1ΔNullPMID:2209806912
FYPO:0002060viable vegetative cell populationMicroscopynot recorded (unrecorded)PMID:117413263730
Microscopylkh1ΔNullPMID:20473289
Microscopylkh1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000183abnormal poly(A)+ mRNA export from nucleusMicroscopylkh1ΔNullPECO:0000004PMID:217454686
FYPO:0000603decreased duration of mitotic G1 phaseFlow cytometry datalkh1ΔNullPMID:233760701
FYPO:0001324decreased protein level during vegetative growth54
affecting adh8Otherlkh1ΔNullPMID:23956636
affecting pgk1Otherlkh1ΔNullPMID:23956636
affecting pyk1Otherlkh1ΔNullPMID:23956636
affecting hsp90Otherlkh1ΔNullPMID:23956636
affecting obr1Otherlkh1ΔNullPMID:23956636
affecting gpm1Otherlkh1ΔNullPMID:23956636
affecting tpx1Otherlkh1ΔNullPMID:23956636
affecting SPBC215.11cOtherlkh1ΔNullPMID:23956636
FYPO:0000584decreased sporulationCell growth assaylkh1ΔNullPECO:0000127, PECO:0000126PMID:2337607048
Cell growth assaylkh1ΔNullPECO:0000126PMID:23376070
FYPO:0001122elongated vegetative cellMicroscopynot recorded (overexpression)PMID:12565823657
FYPO:0002671increased concentration of protein in growth medium1
affecting gas2Otherlkh1ΔNullPMID:23956636
affecting exg1Otherlkh1ΔNullPMID:23956636
affecting bgl2Otherlkh1ΔNullPMID:23956636
affecting psu1Otherlkh1ΔNullPMID:23956636
FYPO:0001327increased protein level during vegetative growth40
affecting ssc1Otherlkh1ΔNullPMID:23956636
affecting ssa2Otherlkh1ΔNullPMID:23956636
affecting mae2Otherlkh1ΔNullPMID:23956636
affecting atd1Otherlkh1ΔNullPMID:23956636
affecting car2Otherlkh1ΔNullPMID:23956636
affecting eno101Otherlkh1ΔNullPMID:23956636
affecting eno102Otherlkh1ΔNullPMID:23956636
affecting SPAC23H3.15cOtherlkh1ΔNullPMID:23956636
affecting fba1Otherlkh1ΔNullPMID:23956636
affecting gpm1Otherlkh1ΔNullPMID:23956636
FYPO:0000783protein mislocalized to cytoplasm17
affecting lkh1Microscopynot recorded (K391A)EndogenousPECO:0000126PMID:21745468
FYPO:0001492viable elongated vegetative cellMicroscopylkh1+ (wild type)OverexpressionPMID:23376070200
FYPO:0000648viable small vegetative cellMicroscopylkh1ΔNullPMID:2337607032
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopylkh1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in lkh1 dual specificity protein kinase Lkh1 PMID:21745468
FYPO affected by mutation in rum1 CDK inhibitor Rum1 PMID:23376070
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1660381656076

UTRs

Region Coordinates Reference
five_prime_UTR660381..658833PMID:21511999
three_prime_UTR656759..656076PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 362 682 109
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 362 682 107
PS50011 Prosite Profiles IPR000719 Protein kinase domain 362 682 110
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 368 391 73
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 484 496 97
PTHR24058:SF0 HMMPANTHER 93 689 1
PTHR24058 HMMPANTHER 93 689 4
1.10.510.10 Gene3D 439 683 112
3.30.200.20 Gene3D 348 438 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 349 685 127

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000517CLK/STY/LAMMEr subfamilyPMID:12565823Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000517

Protein Properties

Ave. residue weight 112.31 Da
Charge 32.00
Isoelectric point 9.30
Molecular weight 77.49 kDa
Number of residues 690
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residueIDAPMID:233760701921
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelincreased during GO:0000281Western blot evidencePMID:21745468
increased during GO:0000087Western blot evidencePMID:21745468

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
5.5during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:12565823433
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
mbx2MADS-box transcription factor Pvg4 Phenotypic SuppressionPMID:22180499
dsk1SR protein-specific kinase Dsk1 Synthetic LethalityPMID:22683458
Phenotypic SuppressionPMID:12565823
Synthetic Lethality
Synthetic Growth DefectPMID:21745468
gsf2galactose-specific flocculin Gsf2 Phenotypic SuppressionPMID:22098069
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
rum1CDK inhibitor Rum1 Reconstituted ComplexPMID:23376070
Affinity Capture-Western
Biochemical Activity
tup11transcriptional corepressor Tup11 Biochemical ActivityPMID:20200159
Reconstituted Complex
Affinity Capture-Western
pmp1dual-specificity MAP kinase phosphatase Pmp1 Two-hybridPMID:23695164
tup12transcriptional corepressor Tup12 Affinity Capture-WesternPMID:20200159
Reconstituted Complex
Biochemical Activity
External References
Database Identifier Description
NBRP SPAC1D4.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1D4.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1D4.11c BioGRID Interaction Datasets
Expression Viewer SPAC1D4.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1D4.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1D4.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1D4.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1D4.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1D4.11c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1D4.11c Cell Cycle Data
GEO SPAC1D4.11c GEO profiles
PInt SPAC1D4.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1D4.11c Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene3361532dual specificity protein kinase Lkh1
EntrezGene3361532dual specificity protein kinase Lkh1
IntEnz2.7.11.1Integrated relational Enzyme database
Rhea2.7.11.1Annotated reactions database
IntEnz2.7.10.1Integrated relational Enzyme database
Rhea2.7.10.1Annotated reactions database
SPD / RIKEN27/27G02Orfeome Localization Data
UniProtKB/SwissProtQ10156Dual specificity protein kinase lkh1
ModBaseQ10156Database of comparative protein structure models
STRINGQ10156Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018187dual specificity protein kinase Lkh1
RefSeq mRNANM_001018423972h- dual specificity protein kinase Lkh1 (lkh1), mRNA
European Nucleotide ArchiveAF334941ENA EMBL mapping
European Nucleotide ArchiveAAK12335ENA Protein Mapping
European Nucleotide ArchiveCAD29835ENA Protein Mapping
European Nucleotide ArchiveCAD29835.2ENA Protein Mapping
UniParcUPI000016201FUniProt Archive

Literature for lkh1

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014