lkh1 (SPAC1D4.11c)

Gene Standard Namelkh1 Characterisation Statuspublished
Systematic IDSPAC1D4.11c Feature Typeprotein coding
Synonymskic1 Name DescriptionLammer Kinase Homolog
Productdual specificity protein kinase Lkh1 Product Size690aa, 77.49 kDa
Genomic Location Chromosome I, 660381-656076 (4306nt); CDS:658832-656760 (2073nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
cellular response to oxidative stress53
Annotation ExtensionEvidenceWith/FromReference
negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of mRNA splicing, via spliceosome2
Annotation ExtensionEvidenceWith/FromReference
protein autophosphorylation18
Annotation ExtensionEvidenceWith/FromReference
protein phosphorylation170
Annotation ExtensionEvidenceWith/FromReference
regulation of barrier septum assembly32
Annotation ExtensionEvidenceWith/FromReference
regulation of cell separation after cytokinesis7
Annotation ExtensionEvidenceWith/FromReference
regulation of fungal-type cell wall organization2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
cone-shaped colony morphologylkh1Δ1
decreased bipolar indexlkh1Δ27
decreased cell population growth during nitrogen starvationlkh1Δ3
decreased cell population growth on glucose carbon sourcelkh1Δ286
decreased sporulation frequencylkh1Δ95
increased duration of lag phaselkh1Δ4
increased flocculationlkh1Δ15
increased monopolar indexlkh1Δ35
sensitive to hydrogen peroxidelkh1Δ141
viable spore populationlkh1Δ45
viable vegetative cell populationlkh1Δ3819

Cell Phenotype

Term NameGenotypesCount
abnormal poly(A)+ mRNA export from nucleuslkh1Δ9
decreased duration of mitotic G1 phaselkh1Δ2
decreased protein level during vegetative growth102
affecting gpm1lkh1Δ
affecting obr1lkh1Δ
affecting pyk1lkh1Δ
affecting hsp90lkh1Δ
affecting pgk1lkh1Δ
affecting adh8lkh1Δ
affecting SPBC215.11clkh1Δ
affecting tpx1lkh1Δ
decreased protein phosphorylation during cellular response to hydrogen peroxide9
affecting csx1lkh1Δ
decreased RNA binding11
affecting csx1 and atf1lkh1Δ
decreased RNA level during cellular response to hydrogen peroxide20
affecting atf1lkh1Δ
elongated vegetative celllkh1+ (wild type)178
increased concentration of protein in growth medium1
affecting psu1lkh1Δ
affecting bgl2lkh1Δ
affecting exg1lkh1Δ
affecting gas2lkh1Δ
increased protein level during vegetative growth86
affecting SPAC23H3.15clkh1Δ
affecting gpm1lkh1Δ
affecting ssc1lkh1Δ
affecting atd1lkh1Δ
affecting eno101lkh1Δ
affecting fba1lkh1Δ
affecting car2lkh1Δ
affecting eno102lkh1Δ
affecting ssa2lkh1Δ
affecting mae2lkh1Δ
normal protein localization to nucleus63
affecting srp2lkh1Δ
affecting prp2lkh1Δ
protein mislocalized to cytoplasm during vegetative growth42
affecting lkh1 (K391A)
viable elongated vegetative celllkh1+ (wild type)306
viable small vegetative celllkh1Δ48
viable vegetative cell with normal cell morphologylkh1Δ3097

Multi-allele phenotypes

Population Phenotype

Term NameGenotypes
decreased cell population growth on glucose carbon sourcelkh1Δ, dsk1Δ
inviable vegetative cell populationlkh1Δ, dsk1-K110A (K110A)
normal vegetative cell population growthdsk1NS (99-160), lkh1Δ

Cell Phenotype

Term NameGenotypes
decreased RNA binding
affecting substrate atf1, affecting activity of csx1sty1Δ, lkh1Δ
viable elongated vegetative celllkh1Δ, prp2+
Target Of
FYPO affected by mutation in lkh1 dual specificity protein kinase Lkh1
FYPO affected by mutation in rum1 CDK inhibitor Rum1
GO substrate of lkh1 dual specificity protein kinase Lkh1
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR660381..658833PMID:21511999
3' UTR656759..656076PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 362 682 108
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 362 682 107
PS50011 Prosite Profiles IPR000719 Protein kinase domain 362 682 110
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 368 391 73
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 484 496 97
PTHR24058:SF0 HMMPANTHER 344 689 1
PTHR24058 HMMPANTHER 344 689 4 Gene3D GPCR kinase 348 438 111
1.10.510.10 Gene3D GPCR kinase 439 683 112
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 349 685 127

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000517CLK/STY/LAMMEr subfamilyPMID:12565823Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000517

Protein Properties

Ave. residue weight 112.31 Da
Charge 32.00
Isoelectric point 9.30
Molecular weight 77.49 kDa
Number of residues 690

Protein Modifications

Term NameResidueCount
phosphorylated residue 1930
Annotation ExtensionEvidenceResidueReference
IDA PMID:23376070
Gene Expression

Qualitative Gene Expression

protein levelincreased during GO:0000281Western blot evidencePMID:21745468
increased during GO:0000087Western blot evidencePMID:21745468

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.5during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
1.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
functionally complemented by S. cerevisiae KNS1full PMID:11741326
gene structure updated PMID:12565823433
Taxonomic Conservation
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3421
conserved in vertebrates3396
conserved in eukaryotes only2503

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load gene that interact physically with SPAC1D4.11c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturescsx1RNA-binding protein Csx1 Affinity Capture-WesternPMID:17612531
affinity capturesrum1CDK inhibitor Rum1 Affinity Capture-WesternPMID:23376070
affinity capturestup11transcriptional corepressor Tup11 Affinity Capture-WesternPMID:20200159
affinity capturestup12transcriptional corepressor Tup12 Affinity Capture-WesternPMID:20200159
modifiestup11transcriptional corepressor Tup11 Biochemical ActivityPMID:20200159
modifiestup12transcriptional corepressor Tup12 Biochemical ActivityPMID:20200159
modifiesrum1CDK inhibitor Rum1 Biochemical ActivityPMID:23376070
forms complex withtup12transcriptional corepressor Tup12 Reconstituted ComplexPMID:20200159
forms complex withtup11transcriptional corepressor Tup11 Reconstituted ComplexPMID:20200159
forms complex withrum1CDK inhibitor Rum1 Reconstituted ComplexPMID:23376070
binds activation domain construct withpmp1dual-specificity MAP kinase phosphatase Pmp1 Two-hybridPMID:23695164
Genetic Interactions

Source: BioGRID

Load gene that interact geneitically with SPAC1D4.11c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withdsk1SR protein-specific kinase Dsk1 Synthetic Growth DefectPMID:21745468
synthetic growth defect withcsx1RNA-binding protein Csx1 Synthetic Growth DefectPMID:17612531
enhances phenotype ofdsk1SR protein-specific kinase Dsk1 Phenotypic EnhancementPMID:17362205
synthetic lethal withdsk1SR protein-specific kinase Dsk1 Synthetic LethalityPMID:12565823
negative genetic interaction withsgf73SAGA complex subunit Sgf73 Negative GeneticPMID:25076038
rescued bygsf2galactose-specific flocculin Gsf2 Phenotypic SuppressionPMID:22098069
rescued bymbx2MADS-box transcription factor Pvg4 Phenotypic SuppressionPMID:22180499
rescued bydsk1SR protein-specific kinase Dsk1 Phenotypic SuppressionPMID:12565823
External References
Database Identifier Description
NBRP SPAC1D4.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1D4.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1D4.11c BioGRID Interaction Datasets
Expression Viewer SPAC1D4.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1D4.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1D4.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1D4.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1D4.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1D4.11c Transcriptome Viewer (Bähler Lab)
GEO SPAC1D4.11c GEO profiles
PInt SPAC1D4.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1D4.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1D4.11c Fission yeast phenotypic data & analysis
Cyclebase SPAC1D4.11c.1 Cell Cycle Data
IntEnz2.7.11.1Integrated relational Enzyme database
Rhea2.7.11.1Annotated reactions database
IntEnz2.7.10.1Integrated relational Enzyme database
Rhea2.7.10.1Annotated reactions database
SPD / RIKEN27/27G02Orfeome Localization Data
UniProtKB/SwissProtQ10156Dual specificity protein kinase lkh1
ModBaseQ10156Database of comparative protein structure models
STRINGQ10156Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018187dual specificity protein kinase Lkh1
RefSeq mRNANM_001018423972h- dual specificity protein kinase Lkh1 (lkh1), mRNA
European Nucleotide ArchiveAF334941ENA EMBL mapping
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveAAK12335ENA Protein Mapping
European Nucleotide ArchiveCAD29835ENA Protein Mapping
European Nucleotide ArchiveCAD29835.2ENA Protein Mapping
UniParcUPI000016201FUniProt Archive

Literature for lkh1

Search: Europe PMC or PubMed

Release Version: PomBase:29_56 - 12 Nov 2015