lkh1 (SPAC1D4.11c)

Gene Standard Namelkh1 Characterisation Statuspublished
Systematic IDSPAC1D4.11c Feature Typeprotein coding
Synonymskic1 Name DescriptionLammer Kinase Homolog
Productdual specificity protein kinase Lkh1 Product Size690aa, 77.49 kDa
Genomic Location Chromosome I, 660381-656076 (4306nt); CDS:658832-656760 (2073nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
cone-shaped colony morphologylkh1Δ1
decreased bipolar indexlkh1Δ27
decreased cell population growth during nitrogen starvationlkh1Δ3
decreased cell population growth on glucose carbon sourcelkh1Δ393
decreased sporulation frequencylkh1Δ103
increased duration of lag phaselkh1Δ4
increased flocculationlkh1Δ34
increased monopolar indexlkh1Δ39
sensitive to hydrogen peroxidelkh1Δ160
slow vegetative cell population growthlkh1Δ369
viable spore populationlkh1Δ46
viable vegetative cell populationlkh1Δ3862

Cell Phenotype

Term NameGenotypesCount
abnormal poly(A)+ mRNA export from nucleuslkh1Δ20
decreased duration of mitotic G1 phaselkh1Δ2
decreased protein level during vegetative growth164
affecting gpm1lkh1Δ
affecting obr1lkh1Δ
affecting pyk1lkh1Δ
affecting hsp90lkh1Δ
affecting pgk1lkh1Δ
affecting adh8lkh1Δ
affecting SPBC215.11clkh1Δ
affecting tpx1lkh1Δ
decreased protein phosphorylation during cellular response to hydrogen peroxide11
affecting csx1lkh1Δ
decreased RNA binding14
affecting csx1 and atf1lkh1Δ
decreased RNA level during cellular response to hydrogen peroxide23
affecting atf1lkh1Δ
elongated multinucleate multiseptate vegetative cell, single septa between nucleilkh1+22
increased concentration of protein in growth medium1
affecting psu1lkh1Δ
affecting bgl2lkh1Δ
affecting exg1lkh1Δ
affecting gas2lkh1Δ
increased protein level during vegetative growth121
affecting SPAC23H3.15clkh1Δ
affecting gpm1lkh1Δ
affecting ssc1lkh1Δ
affecting atd1lkh1Δ
affecting eno101lkh1Δ
affecting fba1lkh1Δ
affecting car2lkh1Δ
affecting eno102lkh1Δ
affecting ssa2lkh1Δ
affecting mae2lkh1Δ
increased septum thicknesslkh1+30
normal mRNA splicing, via spliceosome6
affecting tbp1lkh1Δ
normal protein localization to nucleus during vegetative growth85
affecting srp2lkh1Δ
affecting prp2lkh1Δ
protein mislocalized to cytoplasm during vegetative growth69
affects localization of lkh1 (K391A)
viable elongated vegetative celllkh1+329
viable small vegetative celllkh1Δ56
viable vegetative celllkh1Δ3680
viable vegetative cell with normal cell morphologylkh1Δ3103

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased cell population growth on glucose carbon sourcedsk1Δ, lkh1Δ
inviable vegetative cell populationlkh1Δ, dsk1-K110A (K110A)
irregular colony morphologydsk1Δ, lkh1Δ
normal vegetative cell population growthdsk1NS (99-160), lkh1Δ
slow vegetative cell population growthdsk1Δ, lkh1Δ

Cell Phenotype

Term NameGenotypes
decreased RNA binding
affecting substrate atf1, affecting activity of csx1sty1Δ, lkh1Δ
increased cell wall thickness during vegetative growthdsk1Δ, lkh1Δ
viable elongated vegetative celllkh1Δ, prp2+
Target Of
FYPO affected by mutation in rum1 CDK inhibitor Rum1
FYPO localization affected by mutation in lkh1 dual specificity protein kinase Lkh1
GO substrate of lkh1 dual specificity protein kinase Lkh1
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR660381..658833PMID:21511999
3' UTR656759..656076PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 362 682 108
SM00220 SMART IPR000719 Protein kinase domain 362 682 107
PS50011 Prosite Profiles IPR000719 Protein kinase domain 362 682 108
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 484 496 91
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 368 391 70
PTHR24058:SF0 HMMPANTHER 344 689 1
PTHR24058 HMMPANTHER 344 689 4 Gene3D Immunoglobulin-like fold 348 438 111
1.10.510.10 Gene3D Immunoglobulin-like fold 439 683 112
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 349 685 127

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000517CLK/STY/LAMMEr subfamilyPMID:12565823Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000517

Protein Properties

Ave. residue weight 112.31 Da
Charge 32.00
Codon Adaptation Index 0.40
Isoelectric point 9.30
Molecular weight 77.49 kDa
Number of residues 690

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS34 2293
Annotation ExtensionEvidenceResidueReference
IDA S34 PMID:25720772
phosphorylated residue 2515
Annotation ExtensionEvidenceResidueReference
IDA PMID:23376070
O-phosphorylated residueS32,S34 2461
Annotation ExtensionEvidenceResidueReference
IDA S32,S34 PMID:25720772
Gene Expression

Qualitative Gene Expression

protein levelincreased during GO:0000281Western blot evidencePMID:21745468
increased during GO:0000087Western blot evidencePMID:21745468

Quantitative Gene Expression

View graphical display of gene expression data for lkh1 (SPAC1D4.11c)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.5during GO:0072690PECO:0000126,
population wideexperimental evidencePMID:23101633
1.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
functionally complemented by S. cerevisiae KNS1full PMID:11741326
gene structure updated PMID:12565823435
Taxonomic Conservation
conserved in fungi4608
conserved in eukaryotes4516
conserved in metazoa3498
conserved in vertebrates3473
conserved in eukaryotes only2525

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC1D4.11c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturescsx1RNA-binding protein Csx1 Affinity Capture-WesternPMID:17612531
affinity capturesrum1CDK inhibitor Rum1 Affinity Capture-WesternPMID:23376070
affinity capturestup12transcriptional corepressor Tup12 Affinity Capture-WesternPMID:20200159
affinity capturestup11transcriptional corepressor Tup11 Affinity Capture-WesternPMID:20200159
modifiessce3translation initiation factor (predicted) Biochemical ActivityPMID:26167880
modifiesase1antiparallel microtubule cross-linking factor Ase1 Biochemical ActivityPMID:26167880
modifiescip1RNA-binding protein Cip1 Biochemical ActivityPMID:26167880
modifiesdph2diphthamide biosynthesis protein (predicted) Biochemical ActivityPMID:26167880
modifiesrum1CDK inhibitor Rum1 Biochemical ActivityPMID:23376070
modifiesfma2methionine aminopeptidase Fma2 (predicted) Biochemical ActivityPMID:26167880
modifiesppa2serine/threonine protein phosphatase Ppa2 Biochemical ActivityPMID:26167880
modifiesprp10U2 snRNP-associated protein Sap155 Biochemical ActivityPMID:26167880
modifiessnp27U4/U6 x U5 tri-snRNP complex subunit (predicted) Biochemical ActivityPMID:26167880
modifiessst6ESCRT I complex subunit Vps23 Biochemical ActivityPMID:26167880
modifieshsp9heat shock protein Hsp9 Biochemical ActivityPMID:26167880
modifiestdh1glyceraldehyde-3-phosphate dehydrogenase Tdh1 Biochemical ActivityPMID:26167880
modifiesscw1RNA-binding protein Scw1 Biochemical ActivityPMID:26167880
modifiesSPCC777.02transcription factor (predicted) Biochemical ActivityPMID:26167880
modifiescyk3Nebulin-family actin filament anchoring protein Cyk3 (predicted) Biochemical ActivityPMID:26167880
modifiesspt5DSIF transcription elongation factor complex subunit Spt5 Biochemical ActivityPMID:26167880
modifieseti1conserved fungal protein associated with stress granule Biochemical ActivityPMID:26167880
modifiesSPCC1739.01zf-CCCH type zinc finger protein Biochemical ActivityPMID:26167880
modifiessur2sphingosine hydroxylase Sur2 Biochemical ActivityPMID:26167880
modifiesgim4prefoldin subunit 2, Gim4(predicted) Biochemical ActivityPMID:26167880
modifiesrsv1transcription factor Rsv1 Biochemical ActivityPMID:26167880
modifiescdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Biochemical ActivityPMID:26167880
modifiesrsd1RNA-binding protein Rsd1 (predicted) Biochemical ActivityPMID:26167880
modifiespuf2pumilio family RNA-binding protein Puf2 Biochemical ActivityPMID:26167880
modifiesbye1transcription elongation regulator Bye1 (predicted) Biochemical ActivityPMID:26167880
modifiespub1HECT-type ubiquitin-protein ligase E3 Pub1 Biochemical ActivityPMID:26167880
modifiesscl120S proteasome complex subunit alpha 1 (predicted) Biochemical ActivityPMID:26167880
modifiesrps260140S ribosomal protein S26 (predicted) Biochemical ActivityPMID:26167880
modifiesmsh2MutS protein homolog 2 Biochemical ActivityPMID:26167880
modifiesssl1transcription factor TFIIH complex subunit Ssl1 Biochemical ActivityPMID:26167880
modifiessam1S-adenosylmethionine synthetase Biochemical ActivityPMID:26167880
modifiesvip1RNA-binding protein Vip1 Biochemical ActivityPMID:26167880
modifiesSPBC16H5.12cconserved fungal protein Biochemical ActivityPMID:26167880
modifiesmak2histidine kinase Mak2 Biochemical ActivityPMID:26167880
modifiesask1DASH complex subunit Ask1 Biochemical ActivityPMID:26167880
modifiesrga9RhoGAP, GTPase activating protein Rga9 (predicted) Biochemical ActivityPMID:26167880
modifiesSPCC1322.10cell wall protein Pwp1 Biochemical ActivityPMID:26167880
modifiesrga7RhoGAP, GTPase activating protein Rga7 Biochemical ActivityPMID:26167880
modifiesubp9ubiquitin C-terminal hydrolase Ubp9 Biochemical ActivityPMID:26167880
modifiesTf2-11retrotransposable element/transposon Tf2-type Biochemical ActivityPMID:26167880
modifiesmde5alpha-amylase homolog Mde5 Biochemical ActivityPMID:26167880
modifiesgar2nucleolar protein required for rRNA processing Biochemical ActivityPMID:26167880
modifiesubp2ubiquitin C-terminal hydrolase Ubp2 Biochemical ActivityPMID:26167880
modifiesSPAC26F1.07glucose 1-dehydrogenase (NADP+) (predicted) Biochemical ActivityPMID:26167880
modifiesoga1Stm1 homolog Oga1 Biochemical ActivityPMID:26167880
modifiestho2THO complex subunit Tho2 (predicted) Biochemical ActivityPMID:26167880
modifiespob1Boi family protein Biochemical ActivityPMID:26167880
modifiesrpl340160S ribosomal protein L34 Biochemical ActivityPMID:26167880
modifiesspa2ARF GTPase-activating protein Spa2 Biochemical ActivityPMID:26167880
modifiestup12transcriptional corepressor Tup12 Biochemical ActivityPMID:20200159
modifiessnf30SWI/SNF complex subunit Snf30 Biochemical ActivityPMID:26167880
modifiessnf21ATP-dependent DNA helicase Snf21 Biochemical ActivityPMID:26167880
modifiesmsy1MS calcium ion channel protein Msy1 Biochemical ActivityPMID:26167880
modifiesSPBC418.02NatA N-acetyltransferase complex subunit (predicted) Biochemical ActivityPMID:26167880
modifiestup11transcriptional corepressor Tup11 Biochemical ActivityPMID:20200159
modifiespre620S proteasome complex subunit alpha 4 Pre6 Biochemical ActivityPMID:26167880
modifiesmpr1histidine-containing response regulator phosphotransferase Mpr1 Biochemical ActivityPMID:26167880
modifiessrw1substrate-specific mitotic G1 APC coactivator Srw1/Ste9 Biochemical ActivityPMID:26167880
modifieswis4MAP kinase kinase kinase Wis4 Biochemical ActivityPMID:26167880
modifiesgrh1human GRASP protein family Golgi protein (predicted) Biochemical ActivityPMID:26167880
modifieslkh1dual specificity protein kinase Lkh1 Biochemical ActivityPMID:26167880
modifiesprp22ATP-dependent RNA helicase Prp22 Biochemical ActivityPMID:26167880
modifiesact1actin Act1 Biochemical ActivityPMID:26167880
modifiespic1kinetochore protein, INCENP ortholog Pic1 Biochemical ActivityPMID:26167880
modifiesset11ribosomal protein lysine methyltransferase Set11 Biochemical ActivityPMID:26167880
modifiesSPBC16H5.08cribosome biogenesis ATPase, Arb family ABCF2-like (predicted) Biochemical ActivityPMID:26167880
modifiestif471translation initiation factor eIF4G Biochemical ActivityPMID:26167880
modifiesSPAC11E3.02cC2 domain protein Biochemical ActivityPMID:26167880
modifiesdcp2mRNA decapping complex catalytic subunit Dcp2 Biochemical ActivityPMID:26167880
modifiesdbp10ATP-dependent RNA helicase Dbp10 (predicted) Biochemical ActivityPMID:26167880
modifiesmug123Schizosaccharomyces specific protein Mug123 Biochemical ActivityPMID:26167880
modifieslsd90Lsd90 protein Biochemical ActivityPMID:26167880
modifiesimp2contractile ring protein Imp2 Biochemical ActivityPMID:26167880
modifiesSPAC9E9.06cthreonine synthase (predicted) Biochemical ActivityPMID:26167880
modifiesrpl3560S ribosomal protein L35 Biochemical ActivityPMID:26167880
modifiesasp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Biochemical ActivityPMID:26167880
binds activation domain construct withpmp1dual-specificity MAP kinase phosphatase Pmp1 Two-hybridPMID:23695164
forms complex withrum1CDK inhibitor Rum1 Reconstituted ComplexPMID:23376070
forms complex withtup12transcriptional corepressor Tup12 Reconstituted ComplexPMID:20200159
forms complex withtup11transcriptional corepressor Tup11 Reconstituted ComplexPMID:20200159
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC1D4.11c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withcsx1RNA-binding protein Csx1 Synthetic Growth DefectPMID:17612531
synthetic growth defect withdsk1SR protein-specific kinase Dsk1 Synthetic Growth DefectPMID:10629038
enhances phenotype ofdsk1SR protein-specific kinase Dsk1 Phenotypic EnhancementPMID:17362205
rescued bygsf2cell surface glycoprotein, galactose-specific flocculin Gsf2 Phenotypic SuppressionPMID:22098069
rescued bymbx2MADS-box transcription factor Pvg4 Phenotypic SuppressionPMID:22180499
rescued bydsk1SR protein-specific kinase Dsk1 Phenotypic SuppressionPMID:12565823
synthetic lethal withdsk1SR protein-specific kinase Dsk1 Synthetic LethalityPMID:12565823
negative genetic interaction withsgf73SAGA complex subunit Sgf73 Negative GeneticPMID:25076038
External References
Database Identifier Description
NBRP SPAC1D4.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1D4.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1D4.11c BioGRID Interaction Datasets
Expression Viewer SPAC1D4.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1D4.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1D4.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1D4.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1D4.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1D4.11c Transcriptome Viewer (Bähler Lab)
GEO SPAC1D4.11c GEO profiles
PInt SPAC1D4.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1D4.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1D4.11c Fission yeast phenotypic data & analysis
Cyclebase SPAC1D4.11c.1 Cell Cycle Data
SPD / RIKEN27/27G02Orfeome Localization Data
IntEnz2.7.11.1Integrated relational Enzyme database
Rhea2.7.11.1Annotated reactions database
IntEnz2.7.10.1Integrated relational Enzyme database
Rhea2.7.10.1Annotated reactions database
UniProtKB/SwissProtQ10156Dual specificity protein kinase lkh1
ModBaseQ10156Database of comparative protein structure models
STRINGQ10156Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018187dual specificity protein kinase Lkh1
RefSeq mRNANM_001018423972h- dual specificity protein kinase Lkh1 (lkh1), mRNA
European Nucleotide ArchiveAF334941ENA EMBL mapping
European Nucleotide ArchiveCAD29835.2ENA Protein Mapping
UniParcUPI000016201FUniProt Archive

Literature for lkh1

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017