lkh1 (SPAC1D4.11c)

Gene Standard Namelkh1 ChromosomeI
Systematic IDSPAC1D4.11c Gene Start660381
Synonymskic1 Gene End656076
Productdual specificity protein kinase Lkh1 Gene Length4306
Feature Typeprotein coding CDS Start658832
Name Description CDS End656760
Characterisation Statuspublished CDS Length2073

Ensembl Gene Location
  1. pis1
  2. rtf2
  3. trz1
  4. lkh1
  5. SPNCRNA.671
  6. SPNCRNA.672
  7. SPNCRNA.79
  8. rad15
  9. SPNCRNA.673
Fission Yeast Phenotype Ontology

Ontology Graph

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Term IDTerm NameEvidenceAlleleAllele TypeExpressionConditionReference
FYPO:0000155flocculating cellsCell growth assaylkh1delta (deletion)deletionPMID:22098069
FYPO:0000183abnormal poly(A)+ mRNA export from nucleusMicroscopylkh1delta (deletion)deletionPECO:0000004PMID:21745468
FYPO:0000783protein mislocalized to cytoplasmMicroscopynot recorded (K391A)amino_acid_mutationEndogenousPECO:0000126PMID:21745468
assayed_using lkh1
FYPO:0002060viable vegetative cell populationMicroscopylkh1delta (deletion)deletionPMID:20473289
no_name (unrecorded)unrecordedPMID:11741326
GO Molecular Function

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Term IDTerm NameEvidenceWith/FromReference
GO:0004674protein serine/threonine kinase activityIEAUniProtKB:KW-0723GO_REF:0000037
GO:0004712protein serine/threonine/tyrosine kinase activityIEAEC:2.7.12.1GO_REF:0000003
GO:0004713protein tyrosine kinase activityIEAUniProtKB:KW-0829GO_REF:0000037
GO:0005524ATP bindingIEAUniProtKB:KW-0067GO_REF:0000037
GO Biological Process

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Term IDTerm NameEvidenceWith/FromReference
GO:0006468protein phosphorylationICGO:0004674GO_REF:0000001
GO:0010590regulation of cytokinetic cell separationIMPPMID:12565823
GO:0032955regulation of barrier septum assemblyIMPPMID:12565823
GO:0034599cellular response to oxidative stressIMPPMID:14623292
GO:0035556intracellular signal transduction
has_indirect_input atf1TASPMID:14623292
GO:0048026positive regulation of mRNA splicing, via spliceosomeIMPPMID:21745468
GO:0051519activation of bipolar cell growthIMPPMID:20501954
GO:0060237regulation of fungal-type cell wall organizationIMPPMID:12565823
GO:0060257negative regulation of flocculationIMPPMID:11741326
GO:0060258negative regulation of filamentous growthIMPPMID:11741326
GO Cellular Component

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Term IDTerm NameEvidenceWith/FromReference
GO:0005634nucleusIDAPMID:16823372
requires_direct_regulator lkh1IMPPMID:21745468
Transcript
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Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1660381656076

References

Region Start End Reference
five_prime_UTR660381658833PMID:21511999
three_prime_UTR656759656076PMID:21511999
three_prime_UTR656759656076PMID:21511999
Protein Features
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Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID InterPro Database Description Start End All Genes
PF00069 IPR000719 Pfam Protein kinase, catalytic domain 362 682 109
SM00220 IPR002290 SMART Serine/threonine- / dual specificity protein kinase, catalytic domain 362 682 74
PS50011 IPR000719 Prosite Profiles Protein kinase, catalytic domain 362 682 110
PS00107 IPR017441 Prosite Patterns Protein kinase, ATP binding site 368 391 73
PS00108 IPR008271 Prosite Patterns Serine/threonine-protein kinase, active site 484 496 97
G3DSA:1.10.510.10 Gene3D 439 683 111
G3DSA:3.30.200.20 Gene3D 348 438 108
SSF56112 IPR011009 SuperFamily 349 685 127
PTHR24058 hmmpanther 97 686 4
PTHR24058:SF10 hmmpanther 97 686 1

View domain organization at Pfam

Protein Modifications (PSI-MOD)

Term IDTerm NameEvidenceResidueReference
MOD:00696phosphorylated residue

Manually Curated Family or Sub-families

Term IDTerm NameReferenceAll Genes
PBO:0000517CLK/STY/LAMMEr subfamilyPMID:12565823Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000517

Protein Properties

Ave. residue weight 112.31 Da
Charge 32.00
Isoelectric point 9.30
Molecular weight 77.49 kDa
Number of residues 690
Sequence
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Gene Expression
DescriptionEvidenceDuringConditionRangeAverage per CellReference
protein levelWestern blot evidencecytokinesisincreasedPMID:21745468
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127		population_widePMID:23101633
Western blot evidenceM phase of mitotic cell cycleincreasedPMID:21745468
experimental evidencesingle-celled organism vegetative growth phasePECO:0000005,
PECO:0000014		population_widePMID:23101633
RNA levelexperimental evidencesingle-celled organism vegetative growth phasePECO:0000014,
PECO:0000005		population_wide5.5PMID:23101633
experimental evidencecell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005		population_wide1.4PMID:23101633
Warning
DescriptionQualifierReference
gene structure updated PMID:12565823
Species Distribution
DescriptionQualifierReference
conserved in fungi
conserved in eukaryotes
conserved in metazoa
conserved in vertebrates
conserved in eukaryotes only
Manually curated orthologous groups
SpeciesGeneDescription

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Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
dsk1SR protein-specific kinase Dsk1 Phenotypic SuppressionPMID:12565823
Synthetic Lethality
Synthetic LethalityPMID:22683458
Synthetic Growth DefectPMID:21745468
mbx2MADS-box transcription factor Pvg4 (predicted) Phenotypic SuppressionPMID:22180499
gsf2galactose-specific flocculin Gsf2 Phenotypic SuppressionPMID:22098069
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tup11transcriptional corepressor Tup11 Affinity Capture-WesternPMID:20200159
Reconstituted Complex
Biochemical Activity
rum1CDK inhibitor Rum1 Reconstituted ComplexPMID:23376070
Biochemical Activity
tup12transcriptional corepressor Tup12 Reconstituted ComplexPMID:20200159
Affinity Capture-Western
Biochemical Activity
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External References
Database Identifier Description
NBRP SPAC1D4.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1D4.11c Retrieval of eukaryotic orthologs
BioGrid SPAC1D4.11c BioGRID Interaction Datasets
Bähler Lab SPAC1D4.11c Cell Cycle Expression Profile
Bähler Lab SPAC1D4.11c Meiosis/Sporulation Expression Profies
Bähler Lab SPAC1D4.11c Pheromone response/mating expression profiles
Bähler Lab SPAC1D4.11c Environmental stress expression profiles
Bähler Lab SPAC1D4.11c Bähler Lab Transcriptome Viewer
Cyclebase SPAC1D4.11c Cell Cycle Data
PInt SPAC1D4.11c Bähler Lab Protein-Protein Interaction Predictor
Entrez Gene SPAC1D4.11c Entrez Gene
WikiGene3361532dual specificity protein kinase Lkh1
Uniprot_genenameLKH1
EntrezGene3361532dual specificity protein kinase Lkh1
IntEnz2.7.11.1
IntEnz2.7.10.1
SPD / RIKEN27/27G02Orfeome Localization Data
UniProtKB/SwissProtQ10156Dual specificity protein kinase lkh1
ModBaseQ10156Database of comparative protein structure models
Pfam Protein DomainsQ10156Pfam Domain Arrangement
RefSeq PeptideNP_001018187dual specificity protein kinase Lkh1
RefSeq mRNANM_001018423972h- dual specificity protein kinase Lkh1 (lkh1), mRNA
European Nucleotide ArchiveAAK12335ENA Protein Mapping
European Nucleotide ArchiveCAD29835ENA Protein Mapping
UniParcUPI000016201FUniProt Archive

Literature for lkh1
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Search: UK PMC or PubMed

Release Version: PomBase:18_35 - 11 May 2013