lkh1 (SPAC1D4.11c)

Gene Standard Namelkh1 Characterisation Statuspublished
Systematic IDSPAC1D4.11c Feature Typeprotein coding
Synonymskic1 Name DescriptionLammer Kinase Homolog
Productdual specificity protein kinase Lkh1 Product Size690aa, 77.49 kDa
Genomic Location Chromosome I, 660381-656076 (4306nt); CDS:658832-656760 (2073nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
cellular response to oxidative stress46
Annotation ExtensionEvidenceWith/FromReference
negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of mRNA splicing, via spliceosome2
Annotation ExtensionEvidenceWith/FromReference
protein autophosphorylation18
Annotation ExtensionEvidenceWith/FromReference
protein phosphorylation131
Annotation ExtensionEvidenceWith/FromReference
regulation of barrier septum assembly32
Annotation ExtensionEvidenceWith/FromReference
regulation of cell separation after cytokinesis7
Annotation ExtensionEvidenceWith/FromReference
regulation of fungal-type cell wall organization2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
cone-shaped colony morphologylkh1Δ1
decreased bipolar indexlkh1Δ27
decreased cell population growth during nitrogen starvationlkh1Δ3
decreased cell population growth on glucose carbon sourcelkh1Δ319
decreased sporulation frequencylkh1Δ103
increased duration of lag phaselkh1Δ4
increased flocculationlkh1Δ16
increased monopolar indexlkh1Δ38
sensitive to hydrogen peroxidelkh1Δ154
viable spore populationlkh1Δ46
viable vegetative cell populationlkh1Δ3840

Cell Phenotype

Term NameGenotypesCount
abnormal poly(A)+ mRNA export from nucleuslkh1Δ9
decreased duration of mitotic G1 phaselkh1Δ2
decreased protein level during vegetative growth140
affecting gpm1lkh1Δ
affecting obr1lkh1Δ
affecting pyk1lkh1Δ
affecting hsp90lkh1Δ
affecting pgk1lkh1Δ
affecting adh8lkh1Δ
affecting SPBC215.11clkh1Δ
affecting tpx1lkh1Δ
decreased protein phosphorylation during cellular response to hydrogen peroxide10
affecting csx1lkh1Δ
decreased RNA binding13
affecting csx1 and atf1lkh1Δ
decreased RNA level during cellular response to hydrogen peroxide22
affecting atf1lkh1Δ
elongated vegetative celllkh1+ (wild type)793
increased concentration of protein in growth medium1
affecting psu1lkh1Δ
affecting bgl2lkh1Δ
affecting exg1lkh1Δ
affecting gas2lkh1Δ
increased protein level during vegetative growth104
affecting SPAC23H3.15clkh1Δ
affecting gpm1lkh1Δ
affecting ssc1lkh1Δ
affecting atd1lkh1Δ
affecting eno101lkh1Δ
affecting fba1lkh1Δ
affecting car2lkh1Δ
affecting eno102lkh1Δ
affecting ssa2lkh1Δ
affecting mae2lkh1Δ
normal protein localization to nucleus76
affecting srp2lkh1Δ
affecting prp2lkh1Δ
protein mislocalized to cytoplasm during vegetative growth51
affects localization of lkh1 (K391A)
viable elongated vegetative celllkh1+ (wild type)318
viable small vegetative celllkh1Δ51
viable vegetative cell with normal cell morphologylkh1Δ3100

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased cell population growth on glucose carbon sourcedsk1Δ, lkh1Δ
inviable vegetative cell populationlkh1Δ, dsk1-K110A (K110A)
normal vegetative cell population growthdsk1NS (99-160), lkh1Δ

Cell Phenotype

Term NameGenotypes
decreased RNA binding
affecting substrate atf1, affecting activity of csx1lkh1Δ, sty1Δ
viable elongated vegetative celllkh1Δ, prp2+
Target Of
FYPO affected by mutation in rum1 CDK inhibitor Rum1
FYPO localization affected by mutation in lkh1 dual specificity protein kinase Lkh1
GO substrate of lkh1 dual specificity protein kinase Lkh1
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR660381..658833PMID:21511999
3' UTR656759..656076PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 362 682 108
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 362 682 107
PS50011 Prosite Profiles IPR000719 Protein kinase domain 362 682 110
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 368 391 73
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 484 496 97
PTHR24058 HMMPANTHER 344 689 4
PTHR24058:SF0 HMMPANTHER 344 689 1
1.10.510.10 Gene3D Serine/threonine-protein kinase Rio2 439 683 112 Gene3D Serine/threonine-protein kinase Rio2 348 438 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 349 685 127

View domain organization at Pfam

Term IDTerm NameReferenceCount
PBO:0000517CLK/STY/LAMMEr subfamilyPMID:12565823Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000517

Protein Properties

Ave. residue weight 112.31 Da
Charge 32.00
Codon Adaptation Index 0.40
Isoelectric point 9.30
Molecular weight 77.49 kDa
Number of residues 690

Protein Modifications

Term NameResidueCount
phosphorylated residue 1931
Annotation ExtensionEvidenceResidueReference
IDA PMID:23376070
Gene Expression

Qualitative Gene Expression

protein levelincreased during GO:0000281Western blot evidencePMID:21745468
increased during GO:0000087Western blot evidencePMID:21745468

Quantitative Gene Expression

View graphical display of gene expression data for lkh1 (SPAC1D4.11c)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.5during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
1.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
functionally complemented by S. cerevisiae KNS1full PMID:11741326
gene structure updated PMID:12565823434
Taxonomic Conservation
conserved in fungi4606
conserved in eukaryotes4516
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2506

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC1D4.11c into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturescsx1RNA-binding protein Csx1 Affinity Capture-WesternPMID:17612531
affinity capturesrum1CDK inhibitor Rum1 Affinity Capture-WesternPMID:23376070
affinity capturestup11transcriptional corepressor Tup11 Affinity Capture-WesternPMID:20200159
affinity capturestup12transcriptional corepressor Tup12 Affinity Capture-WesternPMID:20200159
modifiestif471translation initiation factor eIF4G Biochemical ActivityPMID:26167880
modifiesrga7Rho-type GTPase activating protein Rga7 Biochemical ActivityPMID:26167880
modifiesrps260140S ribosomal protein S26 (predicted) Biochemical ActivityPMID:26167880
modifiesdbp10ATP-dependent RNA helicase Dbp10 (predicted) Biochemical ActivityPMID:26167880
modifiesSPCC1322.10cell wall protein Pwp1 Biochemical ActivityPMID:26167880
modifiesgim4prefoldin subunit 2, Gim4(predicted) Biochemical ActivityPMID:26167880
modifiesSPAC11E3.02cC2 domain protein Biochemical ActivityPMID:26167880
modifiesrpl340160S ribosomal protein L34 Biochemical ActivityPMID:26167880
modifiescip1RNA-binding protein Cip1 Biochemical ActivityPMID:26167880
modifiesSPAC9E9.06cthreonine synthase (predicted) Biochemical ActivityPMID:26167880
modifieshsp9heat shock protein Hsp9 Biochemical ActivityPMID:26167880
modifiescyk3cytokinesis protein Cyk3 (predicted) Biochemical ActivityPMID:26167880
modifiesssl1transcription factor TFIIH complex subunit Ssl1 Biochemical ActivityPMID:26167880
modifiesppa2serine/threonine protein phosphatase Ppa2 Biochemical ActivityPMID:26167880
modifiescdc15extended Fer/CIP4 (EFC) domain protein Cdc15 Biochemical ActivityPMID:26167880
modifiessam1S-adenosylmethionine synthetase Biochemical ActivityPMID:26167880
modifiestup12transcriptional corepressor Tup12 Biochemical ActivityPMID:20200159
modifiesdcp2mRNA decapping complex catalytic subunit Dcp2 Biochemical ActivityPMID:26167880
modifieswis4MAP kinase kinase kinase Wis4 Biochemical ActivityPMID:26167880
modifiesvip1RNA-binding protein Vip1 Biochemical ActivityPMID:26167880
modifiesdph2diphthamide biosynthesis protein (predicted) Biochemical ActivityPMID:26167880
modifiesmak2histidine kinase Mak2 Biochemical ActivityPMID:26167880
modifiesrga9RhoGAP, GTPase activator towards Rho/Rac/Cdc42-like small GTPases (predicted) Biochemical ActivityPMID:26167880
modifiesasp1inositol hexakisphosphate kinase/inositol pyrophosphate synthase (predicted) Biochemical ActivityPMID:26167880
modifiesset11ribosomal protein lysine methyltransferase Set11 Biochemical ActivityPMID:26167880
modifieseti1conserved fungal protein associated with stress granule Biochemical ActivityPMID:26167880
modifiesSPBC16H5.12cconserved fungal protein Biochemical ActivityPMID:26167880
modifiesimp2contractile ring protein Imp2 Biochemical ActivityPMID:26167880
modifiessce3translation initiation factor (predicted) Biochemical ActivityPMID:26167880
modifiesrsv1transcription factor Rsv1 Biochemical ActivityPMID:26167880
modifiesoga1Stm1 homolog Oga1 Biochemical ActivityPMID:26167880
modifiesact1actin Act1 Biochemical ActivityPMID:26167880
modifiesprp10U2 snRNP-associated protein Sap155 Biochemical ActivityPMID:26167880
modifiesmsy1MS calcium ion channel protein Msy1 Biochemical ActivityPMID:26167880
modifiesmpr1histidine-containing response regulator phosphotransferase Mpr1 Biochemical ActivityPMID:26167880
modifiesgar2nucleolar protein required for rRNA processing Biochemical ActivityPMID:26167880
modifieslsd90Lsd90 protein Biochemical ActivityPMID:26167880
modifiestho2THO complex subunit Tho2 (predicted) Biochemical ActivityPMID:26167880
modifiesspa2cell polarity protein Spa2 Biochemical ActivityPMID:26167880
modifiespub1HECT-type ubiquitin-protein ligase E3 Pub1 Biochemical ActivityPMID:26167880
modifiessnf21ATP-dependent DNA helicase Snf21 Biochemical ActivityPMID:26167880
modifiesprp22ATP-dependent RNA helicase Prp22 Biochemical ActivityPMID:26167880
modifiesscw1RNA-binding protein Scw1 Biochemical ActivityPMID:26167880
modifiesrpl3560S ribosomal protein L35 Biochemical ActivityPMID:26167880
modifiesSPAC26F1.07glucose 1-dehydrogenase (NADP+) (predicted) Biochemical ActivityPMID:26167880
modifieslkh1dual specificity protein kinase Lkh1 Biochemical ActivityPMID:26167880
modifiespic1kinetochore protein, INCENP ortholog Pic1 Biochemical ActivityPMID:26167880
modifiesSPBC418.02NatA N-acetyltransferase complex subunit (predicted) Biochemical ActivityPMID:26167880
modifiesask1DASH complex subunit Ask1 Biochemical ActivityPMID:26167880
modifiesgrh1human GRASP protein family Golgi protein (predicted) Biochemical ActivityPMID:26167880
modifiespre620S proteasome complex subunit alpha 4 Pre6 Biochemical ActivityPMID:26167880
modifiessnf30SWI/SNF complex subunit Snf30 Biochemical ActivityPMID:26167880
modifiesmug123Schizosaccharomyces specific protein Mug123 Biochemical ActivityPMID:26167880
modifiesubp2ubiquitin C-terminal hydrolase Ubp2 Biochemical ActivityPMID:26167880
modifiesfma2methionine aminopeptidase Fma2 (predicted) Biochemical ActivityPMID:26167880
modifiessst6ESCRT I complex subunit Vps23 Biochemical ActivityPMID:26167880
modifiestup11transcriptional corepressor Tup11 Biochemical ActivityPMID:20200159
modifiesrum1CDK inhibitor Rum1 Biochemical ActivityPMID:23376070
modifiesSPCC645.13transcription elongation regulator (predicted) Biochemical ActivityPMID:26167880
modifiesrsd1RNA-binding protein Rsd1 (predicted) Biochemical ActivityPMID:26167880
modifiesSPCC1739.01zf-CCCH type zinc finger protein Biochemical ActivityPMID:26167880
modifiesmde5alpha-amylase homolog Mde5 Biochemical ActivityPMID:26167880
modifiessur2sphingosine hydroxylase Sur2 Biochemical ActivityPMID:26167880
modifiestdh1glyceraldehyde-3-phosphate dehydrogenase Tdh1 Biochemical ActivityPMID:26167880
modifiesspt5DSIF transcription elongation factor complex subunit Spt5 Biochemical ActivityPMID:26167880
modifiesSPCC162.01cU4/U6 x U5 tri-snRNP complex subunit (predicted) Biochemical ActivityPMID:26167880
modifiesase1antiparallel microtubule cross-linking factor Ase1 Biochemical ActivityPMID:26167880
modifiesSPCC777.02transcription factor (predicted) Biochemical ActivityPMID:26167880
modifiesubp9ubiquitin C-terminal hydrolase Ubp9 Biochemical ActivityPMID:26167880
modifiesmsh2MutS protein homolog 2 Biochemical ActivityPMID:26167880
modifiessrw1fizzy-related CDK inhibitor Srw1 Biochemical ActivityPMID:26167880
modifiesSPBC16H5.08cribosome biogenesis ATPase, Arb family ABCF2-like (predicted) Biochemical ActivityPMID:26167880
modifiesscl120S proteasome complex subunit alpha 1 (predicted) Biochemical ActivityPMID:26167880
modifiespuf2pumilio family RNA-binding protein Puf2 Biochemical ActivityPMID:26167880
modifiesTf2-11retrotransposable element/transposon Tf2-type Biochemical ActivityPMID:26167880
modifiespob1Boi family protein Biochemical ActivityPMID:26167880
forms complex withtup12transcriptional corepressor Tup12 Reconstituted ComplexPMID:20200159
forms complex withtup11transcriptional corepressor Tup11 Reconstituted ComplexPMID:20200159
forms complex withrum1CDK inhibitor Rum1 Reconstituted ComplexPMID:23376070
binds activation domain construct withpmp1dual-specificity MAP kinase phosphatase Pmp1 Two-hybridPMID:26771498
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC1D4.11c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withdsk1SR protein-specific kinase Dsk1 Synthetic LethalityPMID:12565823
rescued bygsf2galactose-specific flocculin Gsf2 Phenotypic SuppressionPMID:22098069
rescued bydsk1SR protein-specific kinase Dsk1 Phenotypic SuppressionPMID:12565823
rescued bymbx2MADS-box transcription factor Pvg4 Phenotypic SuppressionPMID:22180499
negative genetic interaction withsgf73SAGA complex subunit Sgf73 Negative GeneticPMID:25076038
enhances phenotype ofdsk1SR protein-specific kinase Dsk1 Phenotypic EnhancementPMID:17362205
synthetic growth defect withdsk1SR protein-specific kinase Dsk1 Synthetic Growth DefectPMID:21745468
synthetic growth defect withcsx1RNA-binding protein Csx1 Synthetic Growth DefectPMID:17612531
External References
Database Identifier Description
NBRP SPAC1D4.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1D4.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1D4.11c BioGRID Interaction Datasets
Expression Viewer SPAC1D4.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1D4.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1D4.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1D4.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1D4.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1D4.11c Transcriptome Viewer (Bähler Lab)
GEO SPAC1D4.11c GEO profiles
PInt SPAC1D4.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1D4.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1D4.11c Fission yeast phenotypic data & analysis
Cyclebase SPAC1D4.11c.1 Cell Cycle Data
SPD / RIKEN27/27G02Orfeome Localization Data
IntEnz2.7.11.1Integrated relational Enzyme database
Rhea2.7.11.1Annotated reactions database
IntEnz2.7.10.1Integrated relational Enzyme database
Rhea2.7.10.1Annotated reactions database
UniProtKB/SwissProtQ10156Dual specificity protein kinase lkh1
ModBaseQ10156Database of comparative protein structure models
STRINGQ10156Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018187dual specificity protein kinase Lkh1
RefSeq mRNANM_001018423972h- dual specificity protein kinase Lkh1 (lkh1), mRNA

Literature for lkh1

Search: Europe PMC or PubMed

Release Version: PomBase:30_59 - 12 Apr 2016