lkh1 (SPAC1D4.11c)


Gene Standard Namelkh1 Characterisation Statuspublished
Systematic IDSPAC1D4.11c Feature Typeprotein coding
Synonymskic1 Name Description
Productdual specificity protein kinase Lkh1 Product Size690aa, 77.49 kDa
Genomic Location Chromosome I, 660381-656076 (4306nt); CDS:658832-656760 (2073nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0004674protein serine/threonine kinase activity112
has substrate rum1IDAPMID:23376070
GO:0004712protein serine/threonine/tyrosine kinase activityIEA EC:2.7.12.1GO_REF:00000036
GO:0004713protein tyrosine kinase activityIEAUniProtKB-KW:KW-0829GO_REF:00000376
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0051519activation of bipolar cell growthIMPPMID:2050195442
GO:0034599cellular response to oxidative stressIMPPMID:1462329264
GO:0035556intracellular signal transduction138
has indirect input atf1TASPMID:14623292
GO:0031658negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycleIMPPMID:233760701
GO:0060258negative regulation of filamentous growthIMPPMID:117413261
GO:0060257negative regulation of flocculationIMPPMID:117413261
GO:0018107peptidyl-threonine phosphorylationIDAPMID:233760703
GO:0048026positive regulation of mRNA splicing, via spliceosomeIMPPMID:217454682
GO:0032955regulation of barrier septum assemblyIMPPMID:1256582334
GO:0010590regulation of cytokinetic cell separationIMPPMID:1256582312
GO:0060237regulation of fungal-type cell wall organizationIMPPMID:125658233
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005634nucleusIDAPMID:168233722730
dependent on lkh1IMPPMID:21745468
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002296cone-shaped colony morphologyMicroscopylkh1ΔNullPECO:0000137, PECO:0000005PMID:236978061
FYPO:0001407decreased cell population growth on glucose carbon sourceCell growth assaylkh1ΔNullPECO:0000102, PECO:0000137PMID:2337607085
Cell growth assaylkh1ΔNullPECO:0000127, PECO:0000102, PECO:0000126PMID:23376070
FYPO:0000155flocculating cellsCell growth assaylkh1ΔNullPMID:2209806911
FYPO:0002124sensitive to nitrogen starvationCell growth assaylkh1ΔNullPECO:0000127, PECO:0000103, PECO:0000126PMID:233760701
FYPO:0002060viable vegetative cell populationMicroscopylkh1ΔNullPMID:204732893755
no_namePMID:11741326
Microscopylkh1ΔNullPECO:0000137, PECO:0000005PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000183abnormal poly(A)+ mRNA export from nucleusMicroscopylkh1ΔNullPECO:0000004PMID:217454685
FYPO:0000603decreased duration of mitotic G1 phaseFlow cytometry datalkh1ΔNullPMID:233760701
FYPO:0001324decreased protein level during vegetative growthOtherlkh1ΔNullPMID:2395663633
affecting tpx1
affecting gpm1Otherlkh1ΔNullPMID:23956636
affecting pyk1Otherlkh1ΔNullPMID:23956636
affecting adh8Otherlkh1ΔNullPMID:23956636
affecting hsp90Otherlkh1ΔNullPMID:23956636
affecting SPBC215.11cOtherlkh1ΔNullPMID:23956636
affecting obr1Otherlkh1ΔNullPMID:23956636
affecting pgk1Otherlkh1ΔNullPMID:23956636
FYPO:0000584decreased sporulationCell growth assaylkh1ΔNullPECO:0000126PMID:2337607035
Cell growth assaylkh1ΔNullPECO:0000126, PECO:0000127PMID:23376070
FYPO:0001122elongated vegetative cellnot recorded (overexpression)PMID:12565823624
FYPO:0002671increased concentration of protein in growth mediumOtherlkh1ΔNullPMID:239566361
affecting gas2
affecting psu1Otherlkh1ΔNullPMID:23956636
affecting bgl2Otherlkh1ΔNullPMID:23956636
affecting exg1Otherlkh1ΔNullPMID:23956636
FYPO:0001327increased protein level during vegetative growthOtherlkh1ΔNullPMID:2395663627
affecting ssa2
affecting gpm1Otherlkh1ΔNullPMID:23956636
affecting car2Otherlkh1ΔNullPMID:23956636
affecting atd1Otherlkh1ΔNullPMID:23956636
affecting mae2Otherlkh1ΔNullPMID:23956636
affecting eno101Otherlkh1ΔNullPMID:23956636
affecting eno102Otherlkh1ΔNullPMID:23956636
affecting SPAC23H3.15cOtherlkh1ΔNullPMID:23956636
affecting ssc1Otherlkh1ΔNullPMID:23956636
affecting fba1Otherlkh1ΔNullPMID:23956636
FYPO:0000783protein mislocalized to cytoplasmMicroscopynot recorded (K391A)EndogenousPECO:0000126PMID:2174546813
affecting lkh1
FYPO:0001492viable elongated vegetative cellMicroscopylkh1+ (wild type)OverexpressionPMID:23376070170
FYPO:0000648viable small vegetative cellMicroscopylkh1ΔNullPMID:2337607028
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopylkh1ΔNullPECO:0000137, PECO:0000005PMID:236978063078
penetrance FYPO_EXT:0000001
Target Of
RelationshipGeneProductReference
directly regulates lkh1 dual specificity protein kinase Lkh1 PMID:21745468
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1660381656076

UTRs

Region Start End Reference
five_prime_UTR660381658833PMID:21511999
three_prime_UTR656759656076PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 362 682 109
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 362 682 73
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 484 496 97
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 368 391 73
PS50011 Prosite Profiles IPR000719 Protein kinase domain 362 682 110
PTHR24058 HMMPANTHER 142 686 4
PTHR24058:SF10 HMMPANTHER 142 686 1
G3DSA:1.10.510.10 Gene3D 439 683 112
G3DSA:3.30.200.20 Gene3D 348 438 109
SSF56112 SuperFamily IPR011009 349 685 127
Low complexity (SEG) seg 55 65
Low complexity (SEG) seg 127 146
Low complexity (SEG) seg 150 162
Low complexity (SEG) seg 207 217
Low complexity (SEG) seg 307 323

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000517CLK/STY/LAMMEr subfamilyPMID:12565823Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000517

Protein Properties

Ave. residue weight 112.31 Da
Charge 32.00
Isoelectric point 9.30
Molecular weight 77.49 kDa
Number of residues 690
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00696phosphorylated residueIDAPMID:233760701192
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelincreasedWestern blot evidencePMID:21745468
during GO:0000281
increasedWestern blot evidencePMID:21745468
during GO:0000087

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring GO:0072690PECO:0000014,
PECO:0000005
population_wideexperimental evidencePMID:23101633
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population_wideexperimental evidencePMID:23101633
5.5during GO:0072690PECO:0000014,
PECO:0000005
population_wideexperimental evidencePMID:23101633
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:12565823426
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
dsk1SR protein-specific kinase Dsk1 Synthetic LethalityPMID:12565823
Phenotypic Suppression
Synthetic LethalityPMID:22683458
Synthetic Growth DefectPMID:21745468
mbx2MADS-box transcription factor Pvg4 Phenotypic SuppressionPMID:22180499
gsf2galactose-specific flocculin Gsf2 Phenotypic SuppressionPMID:22098069
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
tup11transcriptional corepressor Tup11 Affinity Capture-WesternPMID:20200159
Reconstituted Complex
rum1CDK inhibitor Rum1 Reconstituted ComplexPMID:23376070
Biochemical Activity
Affinity Capture-Western
pmp1dual-specificity MAP kinase phosphatase Pmp1 Two-hybridPMID:23695164
tup12transcriptional corepressor Tup12 Reconstituted ComplexPMID:20200159
Affinity Capture-Western
Biochemical Activity
External References
Database Identifier Description
NBRP SPAC1D4.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1D4.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1D4.11c BioGRID Interaction Datasets
Expression Viewer SPAC1D4.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1D4.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1D4.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1D4.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1D4.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1D4.11c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1D4.11c Cell Cycle Data
GEO SPAC1D4.11c GEO profiles
PInt SPAC1D4.11c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene3361532dual specificity protein kinase Lkh1
EntrezGene3361532dual specificity protein kinase Lkh1
IntEnz2.7.11.1Integrated relational Enzyme database
Rhea2.7.11.1Annotated reactions database
IntEnz2.7.10.1Integrated relational Enzyme database
Rhea2.7.10.1Annotated reactions database
SPD / RIKEN27/27G02Orfeome Localization Data
UniProtKB/SwissProtQ10156Dual specificity protein kinase lkh1
ModBaseQ10156Database of comparative protein structure models
StringQ10156Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018187dual specificity protein kinase Lkh1
RefSeq mRNANM_001018423972h- dual specificity protein kinase Lkh1 (lkh1), mRNA
European Nucleotide ArchiveAAK12335ENA Protein Mapping
European Nucleotide ArchiveCAD29835ENA Protein Mapping
UniParcUPI000016201FUniProt Archive

Literature for lkh1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014