lkh1 (SPAC1D4.11c)


Gene Standard Namelkh1 Characterisation Statuspublished
Systematic IDSPAC1D4.11c Feature Typeprotein coding
Synonymskic1 Name DescriptionLammer Kinase Homolog
Productdual specificity protein kinase Lkh1 Product Size690aa, 77.49 kDa
Genomic Location Chromosome I, 660381-656076 (4306nt); CDS:658832-656760 (2073nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
cellular response to oxidative stress51
Annotation ExtensionEvidenceWith/FromReference
intracellular signal transduction264
Annotation ExtensionEvidenceWith/FromReference
negative regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle1
Annotation ExtensionEvidenceWith/FromReference
positive regulation of mRNA splicing, via spliceosome2
Annotation ExtensionEvidenceWith/FromReference
protein autophosphorylation17
Annotation ExtensionEvidenceWith/FromReference
protein phosphorylation132
Annotation ExtensionEvidenceWith/FromReference
regulation of barrier septum assembly35
Annotation ExtensionEvidenceWith/FromReference
regulation of cell separation after cytokinesis5
Annotation ExtensionEvidenceWith/FromReference
regulation of fungal-type cell wall organization2
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
nucleus2696
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
cone-shaped colony morphologylkh1ΔNull1
decreased bipolar indexlkh1ΔNull27
decreased cell population growth during nitrogen starvationlkh1ΔNull3
decreased cell population growth on glucose carbon sourcelkh1ΔNull258
increased duration of lag phaselkh1ΔNull4
increased flocculationlkh1ΔNull15
increased monopolar indexlkh1ΔNull35
sensitive to hydrogen peroxidelkh1ΔNull139
viable spore populationlkh1ΔNull40
viable vegetative cell populationlkh1ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
abnormal poly(A)+ mRNA export from nucleuslkh1ΔNull9
decreased duration of mitotic G1 phaselkh1ΔNull2
decreased protein level during vegetative growth95
affecting gpm1lkh1ΔNull
affecting obr1lkh1ΔNull
affecting pyk1lkh1ΔNull
affecting hsp90lkh1ΔNull
affecting pgk1lkh1ΔNull
affecting adh8lkh1ΔNull
affecting SPBC215.11clkh1ΔNull
affecting tpx1lkh1ΔNull
decreased protein phosphorylation during cellular response to hydrogen peroxide9
affecting csx1lkh1ΔNull
decreased RNA binding10
affecting csx1 and atf1lkh1ΔNull
decreased RNA level during cellular response to hydrogen peroxide20
affecting atf1lkh1ΔNull
decreased sporulationlkh1ΔNull73
elongated vegetative celllkh1+ (wild type)Overexpression174
increased concentration of protein in growth medium1
affecting psu1lkh1ΔNull
affecting bgl2lkh1ΔNull
affecting exg1lkh1ΔNull
affecting gas2lkh1ΔNull
increased protein level during vegetative growth83
affecting SPAC23H3.15clkh1ΔNull
affecting gpm1lkh1ΔNull
affecting ssc1lkh1ΔNull
affecting atd1lkh1ΔNull
affecting eno101lkh1ΔNull
affecting fba1lkh1ΔNull
affecting car2lkh1ΔNull
affecting eno102lkh1ΔNull
affecting ssa2lkh1ΔNull
affecting mae2lkh1ΔNull
normal protein localization to nucleus55
affecting srp2lkh1ΔNull
affecting prp2lkh1ΔNull
protein mislocalized to cytoplasm36
affecting lkh1unnamed (K391A)Endogenous
viable elongated vegetative celllkh1+ (wild type)Overexpression301
viable small vegetative celllkh1ΔNull46
viable vegetative cell with normal cell morphologylkh1ΔNull3094
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in lkh1 dual specificity protein kinase Lkh1
FYPO affected by mutation in rum1 CDK inhibitor Rum1
GO substrate of lkh1 dual specificity protein kinase Lkh1
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons660381..656076
mRNA660381..656076
5' UTR660381..658833PMID:21511999
CDS658832..656760
3' UTR656759..656076PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 362 682 109
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 362 682 107
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 484 496 97
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 368 391 73
PS50011 Prosite Profiles IPR000719 Protein kinase domain 362 682 110
PTHR24058 HMMPANTHER 93 689 4
PTHR24058:SF0 HMMPANTHER 93 689 1
1.10.510.10 Gene3D 439 683 112
3.30.200.20 Gene3D 348 438 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 349 685 127

View domain organization at Pfam

Manually Curated Family or Sub-families

Term IDTerm NameReferenceCount
PBO:0000517CLK/STY/LAMMEr subfamilyPMID:12565823Temporary processing gif - replaced by AJAX with count of genes annotated with the term PBO:0000517

Protein Properties

Ave. residue weight 112.31 Da
Charge 32.00
Isoelectric point 9.30
Molecular weight 77.49 kDa
Number of residues 690
Modifications

Protein Modifications

Term NameResidueCount
phosphorylated residue 1927
Annotation ExtensionEvidenceResidueReference
IDA PMID:23376070
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
protein levelincreased during GO:0000087Western blot evidencePMID:21745468
increased during GO:0000281Western blot evidencePMID:21745468

Quantitative Gene Expression

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.5during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1.4during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Complementation
DescriptionQualifierReference
functionally complemented by S. cerevisiae KNS1full PMID:11741326
Warning
DescriptionQualifierReferenceCount
gene structure updated PMID:12565823433
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity capturescsx1RNA-binding protein Csx1 Affinity Capture-WesternPMID:17612531
binds activation domain construct withpmp1dual-specificity MAP kinase phosphatase Pmp1 Two-hybridPMID:23695164
modifiesrum1CDK inhibitor Rum1 Biochemical ActivityPMID:23376070
affinity capturesrum1CDK inhibitor Rum1 Affinity Capture-WesternPMID:23376070
forms complex withrum1CDK inhibitor Rum1 Reconstituted ComplexPMID:23376070
modifiestup11transcriptional corepressor Tup11 Biochemical ActivityPMID:20200159
forms complex withtup11transcriptional corepressor Tup11 Reconstituted ComplexPMID:20200159
affinity capturestup11transcriptional corepressor Tup11 Affinity Capture-WesternPMID:20200159
modifiestup12transcriptional corepressor Tup12 Biochemical ActivityPMID:20200159
affinity capturestup12transcriptional corepressor Tup12 Affinity Capture-WesternPMID:20200159
forms complex withtup12transcriptional corepressor Tup12 Reconstituted ComplexPMID:20200159
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic growth defect withcsx1RNA-binding protein Csx1 Synthetic Growth DefectPMID:17612531
synthetic lethal withdsk1SR protein-specific kinase Dsk1 Synthetic LethalityPMID:22683458
rescued bydsk1SR protein-specific kinase Dsk1 Phenotypic SuppressionPMID:12565823
synthetic lethal withdsk1SR protein-specific kinase Dsk1 Synthetic LethalityPMID:12565823
synthetic growth defect withdsk1SR protein-specific kinase Dsk1 Synthetic Growth DefectPMID:21745468
enhances phenotype ofdsk1SR protein-specific kinase Dsk1 Phenotypic EnhancementPMID:17362205
rescued bygsf2galactose-specific flocculin Gsf2 Phenotypic SuppressionPMID:22098069
rescued bymbx2MADS-box transcription factor Pvg4 Phenotypic SuppressionPMID:22180499
negative genetic interaction withsgf73SAGA complex subunit Sgf73 Negative GeneticPMID:25076038
External References
Database Identifier Description
NBRP SPAC1D4.11c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1D4.11c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1D4.11c BioGRID Interaction Datasets
Expression Viewer SPAC1D4.11c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1D4.11c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1D4.11c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1D4.11c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1D4.11c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1D4.11c Transcriptome Viewer (Bähler Lab)
GEO SPAC1D4.11c GEO profiles
PInt SPAC1D4.11c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1D4.11c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1D4.11c Fission yeast phenotypic data & analysis
Cyclebase SPAC1D4.11c.1 Cell Cycle Data
SPD / RIKEN27/27G02Orfeome Localization Data
IntEnz2.7.11.1Integrated relational Enzyme database
Rhea2.7.11.1Annotated reactions database
IntEnz2.7.10.1Integrated relational Enzyme database
Rhea2.7.10.1Annotated reactions database
UniProtKB/SwissProtQ10156Dual specificity protein kinase lkh1
ModBaseQ10156Database of comparative protein structure models
STRINGQ10156Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_001018187dual specificity protein kinase Lkh1
RefSeq mRNANM_001018423972h- dual specificity protein kinase Lkh1 (lkh1), mRNA
European Nucleotide ArchiveAF334941ENA EMBL mapping
European Nucleotide ArchiveCAD29835.2ENA Protein Mapping
UniParcUPI000016201FUniProt Archive

Literature for lkh1

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015