byr1 (SPAC1D4.13)


Gene Standard Namebyr1 Characterisation Statuspublished
Systematic IDSPAC1D4.13 Feature Typeprotein coding
Synonymsste1, ste3 Name DescriptionSTErile,
BYpass of Ras
ProductMAP kinase kinase Byr1 Product Size340aa, 38.19 kDa
Genomic Location Chromosome I, 667815-669742 (1928nt); CDS:668153-669175 (1023nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0004708MAP kinase kinase activity3
has substrate spk1IDAPMID:11683500
GO:0005515protein bindingIPIbob1PMID:11683500831
GO:0004674protein serine/threonine kinase activityIEAIPR008271GO_REF:0000002112
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000165MAPK cascadeIDAPMID:1168350022
GO:0000187activation of MAPK activityIDAPMID:116835003
GO:0030437ascospore formationIMPPMID:304238675
GO:0000751cell cycle arrest in response to pheromoneTASPMID:84434065
GO:0000747conjugation with cellular fusionIMPPMID:3042386110
GO:0000750pheromone-dependent signal transduction involved in conjugation with cellular fusionIMPPMID:797589419
GO:0006468protein phosphorylationIDAPMID:11683500105
GO:0032005signal transduction involved in conjugation with cellular fusionIMPPMID:819663126
IGIbyr2PMID:8443406
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032153cell division siteIDAPMID:11683500301
GO:0051286cell tipIDAPMID:11683500190
GO:0005829cytosolIDAPMID:168233722315
GO:0005634nucleusIDAPMID:168233722740
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001128decreased septation indexMicroscopybyr1ΔNullPECO:0000005PMID:2050195419
FYPO:0000637increased cell population growth rate on glucose carbon sourceCell growth assaybyr1ΔNullPECO:0000137PMID:30423867
Cell growth assaybyr1ΔNullPECO:0000102PMID:9135147
Cell growth assaybyr1ΔNullPECO:0000102, PECO:0000092PMID:9135147
FYPO:0001164normal growth on glucose carbon sourceCell growth assayK93M (K93M)Not specifiedPMID:2300054176
Cell growth assayE229K (E229K)Not specifiedPMID:2300054
FYPO:0000964normal growth on thiabendazoleCell growth assaybyr1ΔNullPECO:0000102, PECO:0000137, PECO:0000005PMID:116835009
FYPO:0002060viable vegetative cell populationMicroscopybyr1ΔNullPMID:204732893759
Microscopybyr1ΔNullPMID:15821139
Microscopybyr1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001996RNA absent from cell during nitrogen starvation12
affecting fus1Transcript expression level evidencebyr1ΔNullPMID:7791776
FYPO:0000302abnormal response to pheromoneMicroscopyste3-JM66Not specifiedPMID:193412119
Microscopyste1-K22Not specifiedPMID:1934121
Microscopyste1-OG15Not specifiedPMID:1934121
FYPO:0000583abolished sporulationCell growth assaybyr1-/byr1- (homozygous diploid)Not specifiedPMID:204666951
Cell growth assayhomozygous diploid (byr1::LEU2/byr1::LEU2)Not specifiedPMID:3042386
FYPO:0000822decreased concentration of P-factor in growth mediumMicroscopyste3-JM66Not specifiedPMID:19341219
Microscopyste1-OG15Not specifiedPMID:1934121
Microscopyste1-K22Not specifiedPMID:1934121
FYPO:0000708decreased mating efficiencyhigh throughput nucleotide sequencing assay evidencebyr1ΔNullPECO:0000015PMID:23950735240
FYPO:0001283decreased protein level during cellular response to nitrogen starvation10
affecting mat2-PiReporter gene assaysterilebyr1Not specifiedPMID:7975894
FYPO:0002375decreased protein tyrosine phosphorylation6
affecting spk1Western blot assaybyr1ΔNullPMID:8413241
FYPO:0002938normal mRNA level during vegetative growth9
affecting ras1Transcript expression level evidenceK93M (K93M)Not specifiedPMID:2300054
FYPO:0001147normal mating efficiencyMicroscopybyr1+ (wild type)OverexpressionPMID:304238620
FYPO:0002442normal protein localization to cell division site27
affecting bob1Microscopybyr1ΔNullPMID:11683500
FYPO:0001587normal protein localization to cell tip17
affecting byr1Microscopybyr1ΔNullPMID:11683500
FYPO:0001315normal vegetative cell morphologyMicroscopybyr1ΔNullPECO:0000137PMID:30423863573
FYPO:0000280sterileOtherste1-OG15Not specifiedPMID:318551450
Cell growth assaybyr1ΔNullPMID:23370392
Cell growth assaybyr1ΔNullPMID:3042386
Otherste3-JM66Not specifiedPMID:3185514
Cell growth assaybyr1ΔNullPMID:9133664
Microscopybyr1ΔNullPMID:2046669
MicroscopyK93M (K93M)Not specifiedPMID:2300054
MicroscopyE229K (E229K)Not specifiedPMID:2300054
Microscopybyr1ΔNullPMID:11683500
FYPO:0001491viable vegetative cellCell growth assaybyr1ΔNullPMID:30423863607
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopyK93M (K93M)Not specifiedPMID:23000543089
Cell growth assayE229K (E229K)Not specifiedPMID:2300054
penetrance FYPO_EXT:0000001Microscopybyr1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in ras1 GTPase Ras1 PMID:2300054
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
1667815669742

UTRs

Region Coordinates Reference
five_prime_UTR667815..668152PMID:21511999
three_prime_UTR669176..669742PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 67 320 109
SM00220 SMART IPR002290 Serine/threonine- /dual specificity protein kinase, catalytic domain 66 320 107
PS50011 Prosite Profiles IPR000719 Protein kinase domain 66 320 110
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 182 194 97
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 72 93 73
PTHR24360 HMMPANTHER 57 334 3
PTHR24360:SF16 HMMPANTHER 57 334 2
3.30.200.20 Gene3D 63 127 111
1.10.510.10 Gene3D 128 323 112
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 66 326 127
Coil ncoils Rabaptin coiled-coil domain 27 48 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.32 Da
Charge 5.00
Isoelectric point 7.52
Molecular weight 38.19 kDa
Number of residues 340
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelunchanged during GO:0060274Northern assay evidencePMID:2300054
present during GO:0072690Northern assay evidencePMID:2300054

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3354.33during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
4426during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.7during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
orthologs cannot be distinguished130
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
wis1MAP kinase kinase Wis1 Synthetic Growth DefectPMID:8557102
ras1GTPase Ras1 Phenotypic SuppressionPMID:10395922
Dosage RescuePMID:3042386
Phenotypic SuppressionPMID:9133664
Phenotypic SuppressionPMID:1515675
zfs1CCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism Phenotypic SuppressionPMID:8534915
ste4adaptor protein Ste4 Phenotypic SuppressionPMID:8816472
fan1Fanconi-associated nuclease Fan1 Synthetic LethalityPMID:24192486
bob1prefoldin subunit 5 (predicted) Phenotypic SuppressionPMID:11683500
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
SPBC56F2.07cribosome biogenesis factor recycling AAA family ATPase (predicted) Two-hybridPMID:23695164
moc3transcription factor Moc3 Two-hybridPMID:23695164
cbh1CENP-B homolog Cbh1 Two-hybridPMID:23695164
cbh2CENP-B homolog Cbh2 Two-hybridPMID:23695164
spk1MAP kinase Spk1 Two-hybridPMID:9660817
Two-hybridPMID:23695164
bob1prefoldin subunit 5 (predicted) Two-hybridPMID:11683500
Affinity Capture-Western
byr2MAP kinase kinase kinase Byr2 Two-hybridPMID:9315645
Two-hybridPMID:23695164
External References
Database Identifier Description
NBRP SPAC1D4.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1D4.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1D4.13 BioGRID Interaction Datasets
Expression Viewer SPAC1D4.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1D4.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1D4.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1D4.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1D4.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1D4.13 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1D4.13 Cell Cycle Data
GEO SPAC1D4.13 GEO profiles
PInt SPAC1D4.13 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1D4.13 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1D4.13 Fission yeast phenotypic data & analysis
SPD / RIKEN15/15C10Orfeome Localization Data
IntEnz2.7.1.-Integrated relational Enzyme database
Rhea2.7.1.-Annotated reactions database
UniProtKB/SwissProtP10506Protein kinase byr1
ModBaseP10506Database of comparative protein structure models
STRINGP10506Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593026MAP kinase kinase Byr1
RefSeq mRNANM_001018425972h- MAP kinase kinase Byr1 (byr1), mRNA
European Nucleotide ArchiveCAA30326.1ENA Protein Mapping
European Nucleotide ArchiveCAA93222.1ENA Protein Mapping
UniParcUPI0000126B8FUniProt Archive

Literature for byr1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014