byr1 (SPAC1D4.13)

Gene Standard Namebyr1 Characterisation Statuspublished
Systematic IDSPAC1D4.13 Feature Typeprotein coding
Synonymsste1, ste3 Name DescriptionSTErile,
BYpass of Ras
ProductMAP kinase kinase Byr1 Product Size340aa, 38.19 kDa
Genomic Location Chromosome I, 667815-669742 (1928nt); CDS:668153-669175 (1023nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
MAP kinase kinase activity3
Annotation ExtensionEvidenceWith/FromReference
protein binding854
Annotation ExtensionEvidenceWith/FromReference
protein serine/threonine kinase activity114
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
activation of MAPK activity3
Annotation ExtensionEvidenceWith/FromReference
ascospore formation68
Annotation ExtensionEvidenceWith/FromReference
conjugation with cellular fusion111
Annotation ExtensionEvidenceWith/FromReference
MAPK cascade26
Annotation ExtensionEvidenceWith/FromReference
MAPK cascade involved in ascospore formation5
Annotation ExtensionEvidenceWith/FromReference
MAPK cascade involved in conjugation with cellular fusion5
Annotation ExtensionEvidenceWith/FromReference
mitotic cell cycle arrest in response to pheromone6
Annotation ExtensionEvidenceWith/FromReference
pheromone-dependent signal transduction involved in conjugation with cellular fusion19
Annotation ExtensionEvidenceWith/FromReference
protein phosphorylation119
Annotation ExtensionEvidenceWith/FromReference
signal transduction involved in conjugation with cellular fusion29
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site305
Annotation ExtensionEvidenceWith/FromReference
cell tip194
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased mating efficiencybyr1ΔNull249
decreased septation indexbyr1ΔNull20
increased cell population growth rate on glucose carbon sourcebyr1ΔNull11
normal growth on glucose carbon sourceE229K (E229K)Not specified200
K93M (K93M)Not specified
normal growth on thiabendazolebyr1ΔNull11
normal mating efficiencybyr1+ (wild type)Overexpression23
viable vegetative cell populationbyr1ΔNull3792

Cell Phenotype

Term NameAlleleExpressionCount
abnormal response to pheromoneste1-K22Not specified23
ste1-OG15Not specified
ste3-JM66Not specified
abolished sporulationbyr1-/byr1- (homozygous diploid)Not specified54
homozygous diploid (byr1::LEU2/byr1::LEU2)Not specified
decreased concentration of P-factor in growth mediumste1-K22Not specified9
ste1-OG15Not specified
ste3-JM66Not specified
decreased protein level during cellular response to nitrogen starvation20
affecting mat2-Pisterilebyr1Not specified
decreased protein tyrosine phosphorylation10
affecting spk1byr1ΔNull
normal mRNA level during vegetative growth11
affecting ras1K93M (K93M)Not specified
normal protein localization to cell division site28
affecting bob1byr1ΔNull
normal protein localization to cell tip21
affecting byr1byr1ΔNull
normal vegetative cell morphologybyr1ΔNull3581
RNA absent from cell during nitrogen starvation14
affecting fus1byr1ΔNull
E229K (E229K)Not specified
K93M (K93M)Not specified
ste1-OG15Not specified
ste3-JM66Not specified
viable vegetative cellbyr1ΔNull3627
viable vegetative cell with normal cell morphologybyr1ΔNull3092
E229K (E229K)Not specified
K93M (K93M)Not specified
Target Of
FYPO affected by mutation in ras1 GTPase Ras1 PMID:2300054
GO substrate of byr2 MAP kinase kinase kinase Byr2 PMID:8443406
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR667815..668152PMID:21511999
3' UTR669176..669742PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 67 320 109
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 66 320 107
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 182 194 97
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 72 93 73
PS50011 Prosite Profiles IPR000719 Protein kinase domain 66 320 110
PTHR24360 HMMPANTHER 28 334 3 Gene3D 63 127 111
1.10.510.10 Gene3D 128 323 112
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 66 326 127
Coil ncoils Rabaptin coiled-coil domain 27 48 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.32 Da
Charge 5.00
Isoelectric point 7.52
Molecular weight 38.19 kDa
Number of residues 340
Gene Expression

Qualitative Gene Expression

RNA levelunchanged during GO:0060274Northern assay evidencePMID:2300054
present during GO:0072690Northern assay evidencePMID:2300054

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4426during GO:0072690PECO:0000126,
single cellmass spectrometry evidencePMID:24763107
3354.33during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.7during GO:0072690PECO:0000005,
population wideexperimental evidencePMID:23101633
0.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population wideexperimental evidencePMID:23101633
functionally complements S. cerevisiae STE7partial PMID:8443406
Species Distribution
orthologs cannot be distinguished130
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3430
conserved in vertebrates3405
conserved in eukaryotes only2502

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withbob1prefoldin subunit 5 (predicted) Two-hybridPMID:11683500
affinity capturesbob1prefoldin subunit 5 (predicted) Affinity Capture-WesternPMID:11683500
binds DNA-binding domain construct withbob1prefoldin subunit 5 (predicted) Two-hybridPMID:11683500
binds activation domain construct withbyr2MAP kinase kinase kinase Byr2 Two-hybridPMID:9315645
binds activation domain construct withbyr2MAP kinase kinase kinase Byr2 Two-hybridPMID:23695164
binds activation domain construct withcbh1CENP-B homolog Cbh1 Two-hybridPMID:23695164
binds activation domain construct withcbh2CENP-B homolog Cbh2 Two-hybridPMID:23695164
binds activation domain construct withmoc3transcription factor Moc3 Two-hybridPMID:23695164
binds activation domain construct withSPBC56F2.07cribosome biogenesis factor recycling AAA family ATPase (predicted) Two-hybridPMID:23695164
binds activation domain construct withspk1MAP kinase Spk1 Two-hybridPMID:9660817
binds activation domain construct withspk1MAP kinase Spk1 Two-hybridPMID:23695164
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rescuesbob1prefoldin subunit 5 (predicted) Phenotypic SuppressionPMID:11683500
rescuesbyr2MAP kinase kinase kinase Byr2 Phenotypic SuppressionPMID:15713656
synthetic lethal withfan1Fanconi-associated nuclease Fan1 Synthetic LethalityPMID:24192486
rescuesgpa1G-protein alpha subunit Phenotypic SuppressionPMID:8443406
rescuesras1GTPase Ras1 Phenotypic SuppressionPMID:10395922
overexpression rescuesras1GTPase Ras1 Dosage RescuePMID:3042386
rescuesras1GTPase Ras1 Phenotypic SuppressionPMID:9133664
rescuesras1GTPase Ras1 Phenotypic SuppressionPMID:1515675
rescued bysla1La protein homolog Phenotypic SuppressionPMID:14665462
rescuesste4adaptor protein Ste4 Phenotypic SuppressionPMID:8816472
synthetic growth defect withwis1MAP kinase kinase Wis1 Synthetic Growth DefectPMID:8557102
rescueszfs1CCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism Phenotypic SuppressionPMID:8534915
External References
Database Identifier Description
NBRP SPAC1D4.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1D4.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1D4.13 BioGRID Interaction Datasets
Expression Viewer SPAC1D4.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1D4.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1D4.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1D4.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1D4.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1D4.13 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1D4.13 Cell Cycle Data
GEO SPAC1D4.13 GEO profiles
PInt SPAC1D4.13 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1D4.13 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1D4.13 Fission yeast phenotypic data & analysis
SPD / RIKEN15/15C10Orfeome Localization Data
IntEnz2.7.1.-Integrated relational Enzyme database
Rhea2.7.1.-Annotated reactions database
UniProtKB/SwissProtP10506Protein kinase byr1
ModBaseP10506Database of comparative protein structure models
STRINGP10506Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593026MAP kinase kinase Byr1
RefSeq mRNANM_001018425972h- MAP kinase kinase Byr1 (byr1), mRNA
European Nucleotide ArchiveCAA30326.1ENA Protein Mapping
European Nucleotide ArchiveCAA93222.1ENA Protein Mapping
UniParcUPI0000126B8FUniProt Archive

Literature for byr1

Search: Europe PMC or PubMed

Release Version: PomBase:25_50 - 10 Mar 2015