byr1 (SPAC1D4.13)

Gene Standard Namebyr1 Characterisation Statuspublished
Systematic IDSPAC1D4.13 Feature Typeprotein coding
Synonymsste1, ste3 Name DescriptionSTErile,
BYpass of Ras
ProductMAP kinase kinase Byr1 Product Size340aa, 38.19 kDa
Genomic Location Chromosome I, 667815-669742 (1928nt); CDS:668153-669175 (1023nt)

Ensembl Gene Location
GO Molecular Function

GO Slim Terms


Term NameCount
ATP binding559
Annotation ExtensionEvidenceWith/FromReference
MAP kinase kinase activity7
Annotation ExtensionEvidenceWith/FromReference
protein binding866
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ascospore formation62
Annotation ExtensionEvidenceWith/FromReference
conjugation with cellular fusion114
Annotation ExtensionEvidenceWith/FromReference
MAPK cascade involved in ascospore formation5
Annotation ExtensionEvidenceWith/FromReference
MAPK cascade involved in conjugation with cellular fusion5
Annotation ExtensionEvidenceWith/FromReference
mitotic cell cycle arrest in response to pheromone6
Annotation ExtensionEvidenceWith/FromReference
pheromone-dependent signal transduction involved in conjugation with cellular fusion19
Annotation ExtensionEvidenceWith/FromReference
protein phosphorylation179
Annotation ExtensionEvidenceWith/FromReference
signal transduction involved in conjugation with cellular fusion29
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site308
Annotation ExtensionEvidenceWith/FromReference
cell tip199
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencybyr1Δ252
decreased septation indexbyr1Δ20
increased cell population growth rate on glucose carbon sourcebyr1Δ12
normal growth on glucose carbon sourceE229K (E229K)225
K93M (K93M)
normal growth on thiabendazolebyr1Δ20
normal mating efficiencybyr1+25
viable vegetative cell populationbyr1Δ3815

Cell Phenotype

Term NameGenotypesCount
abnormal response to pheromoneste1-K2225
abolished sporulationbyr1-/byr1- (homozygous diploid)54
homozygous diploid (byr1::LEU2/byr1::LEU2)
decreased concentration of P-factor in growth mediumste1-OG159
decreased protein level during cellular response to nitrogen starvation20
affecting mat2-Pisterilebyr1
decreased protein tyrosine phosphorylation10
affecting spk1byr1Δ
normal mRNA level during vegetative growth12
affecting ras1K93M (K93M)
normal protein localization to cell division site31
affecting bob1byr1Δ
normal protein localization to cell tip22
affecting byr1byr1Δ
normal vegetative cell morphologybyr1Δ551
RNA absent from cell during nitrogen starvation15
affecting fus1byr1Δ
sterileE229K (E229K)56
K93M (K93M)
viable vegetative cellbyr1Δ3639
viable vegetative cell with normal cell morphologyK93M (K93M)3095
E229K (E229K)

Multi-allele phenotypes

Population Phenotype

Term NameGenotypes
decreased mating efficiencyras1-S40, byr1+
normal mating efficiencybob1Δ, byr1+

Cell Phenotype

Term NameGenotypes
abnormal sporulationbyr1-/byr1- (homozygous diploid), byr2+
meiosis and sporulation in haploidpat1-114, ste1-OG15
ste3-JM66, pat1-114
sterilezfs1+, byr1Δ
byr1Δ, bob1+
byr1Δ, byr2+
Target Of
FYPO affected by mutation in ras1 GTPase Ras1
GO substrate of byr2 MAP kinase kinase kinase Byr2
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR667815..668152PMID:21511999
3' UTR669176..669742PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00069 Pfam IPR000719 Protein kinase domain 67 320 108
SM00220 SMART IPR002290 Serine/threonine/dual specificity protein kinase, catalytic domain 66 320 107
PS00107 Prosite Patterns IPR017441 Protein kinase, ATP binding site 72 93 73
PS00108 Prosite Patterns IPR008271 Serine/threonine-protein kinase, active site 182 194 97
PS50011 Prosite Profiles IPR000719 Protein kinase domain 66 320 110
PTHR24360 HMMPANTHER 28 334 3
1.10.510.10 Gene3D ALK tyrosine kinase receptor 128 323 112 Gene3D Ephrin receptor, transmembrane domain 63 127 111
SSF56112 SuperFamily IPR011009 Protein kinase-like domain 66 326 127
Coil ncoils Rabaptin coiled-coil domain 27 47 1048

View domain organization at Pfam

Protein Properties

Ave. residue weight 112.32 Da
Charge 5.00
Codon Adpatation Index 0.40
Isoelectric point 7.52
Molecular weight 38.19 kDa
Number of residues 340
Gene Expression

Qualitative Gene Expression

RNA levelunchanged during GO:0060274Northern assay evidencePMID:2300054
present during GO:0072690Northern assay evidencePMID:2300054

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
3354.33during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
4426during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
2.7during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
0.84during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
functionally complements S. cerevisiae STE7partial PMID:8443406
Taxonomic Conservation
orthologs cannot be distinguished129
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3421
conserved in vertebrates3396
conserved in eukaryotes only2503

Manually curated orthologous groups


Orthologs in Compara

Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
binds activation domain construct withbob1prefoldin subunit 5 (predicted) Two-hybridPMID:11683500
affinity capturesbob1prefoldin subunit 5 (predicted) Affinity Capture-WesternPMID:11683500
binds DNA-binding domain construct withbob1prefoldin subunit 5 (predicted) Two-hybridPMID:11683500
binds activation domain construct withbyr2MAP kinase kinase kinase Byr2 Two-hybridPMID:23695164
binds activation domain construct withbyr2MAP kinase kinase kinase Byr2 Two-hybridPMID:9315645
binds activation domain construct withcbh1CENP-B homolog Cbh1 Two-hybridPMID:23695164
binds activation domain construct withcbh2CENP-B homolog Cbh2 Two-hybridPMID:23695164
binds activation domain construct withmoc3transcription factor Moc3 Two-hybridPMID:23695164
binds activation domain construct withSPBC56F2.07cribosome biogenesis factor recycling AAA family ATPase (predicted) Two-hybridPMID:23695164
binds activation domain construct withspk1MAP kinase Spk1 Two-hybridPMID:23695164
binds activation domain construct withspk1MAP kinase Spk1 Two-hybridPMID:9660817
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
rescuesbyr2MAP kinase kinase kinase Byr2 Phenotypic SuppressionPMID:15713656
synthetic lethal withfan1Fanconi-associated nuclease Fan1 Synthetic LethalityPMID:24192486
rescuesgpa1G-protein alpha subunit Phenotypic SuppressionPMID:8443406
rescuesras1GTPase Ras1 Phenotypic SuppressionPMID:9133664
rescuesras1GTPase Ras1 Phenotypic SuppressionPMID:1515675
overexpression rescuesras1GTPase Ras1 Dosage RescuePMID:3042386
rescued bysla1La protein homolog Phenotypic SuppressionPMID:14665462
rescuesste4adaptor protein Ste4 Phenotypic SuppressionPMID:8816472
synthetic growth defect withwis1MAP kinase kinase Wis1 Synthetic Growth DefectPMID:8557102
rescueszfs1CCCH tandem zinc finger protein, human Tristetraprolin homolog Zfs1, involved in mRNA catabolism Phenotypic SuppressionPMID:8534915
External References
Database Identifier Description
NBRP SPAC1D4.13 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1D4.13 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1D4.13 BioGRID Interaction Datasets
Expression Viewer SPAC1D4.13 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1D4.13 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1D4.13 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1D4.13 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1D4.13 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1D4.13 Transcriptome Viewer (Bähler Lab)
GEO SPAC1D4.13 GEO profiles
PInt SPAC1D4.13 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1D4.13 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1D4.13 Fission yeast phenotypic data & analysis
Cyclebase SPAC1D4.13.1 Cell Cycle Data
IntEnz2.7.1.-Integrated relational Enzyme database
Rhea2.7.1.-Annotated reactions database
SPD / RIKEN15/15C10Orfeome Localization Data
UniProtKB/SwissProtP10506Protein kinase byr1
ModBaseP10506Database of comparative protein structure models
STRINGP10506Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_593026MAP kinase kinase Byr1
RefSeq mRNANM_001018425972h- MAP kinase kinase Byr1 (byr1), mRNA
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveX07445ENA EMBL mapping
European Nucleotide ArchiveCAA30326ENA Protein Mapping
European Nucleotide ArchiveCAA30326.1ENA Protein Mapping
European Nucleotide ArchiveCAA93222ENA Protein Mapping
European Nucleotide ArchiveCAA93222.1ENA Protein Mapping
UniParcUPI0000126B8FUniProt Archive

Literature for byr1

Search: Europe PMC or PubMed

Release Version: PomBase:28_55 - 09 Sep 2015