cdc22 (SPAC1F7.05)


Gene Standard Namecdc22 Characterisation Statuspublished
Systematic IDSPAC1F7.05 Feature Typeprotein coding
Synonyms Name Description
Productribonucleoside reductase large subunit Cdc22 Product Size811aa, 92.00 kDa
Genomic Location Chromosome I, 4226958-4229989 (3032nt); CDS:4226982-4229733 (2752nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037558
GO:0051063CDP reductase activity2
inhibited by dATPIDAPMID:16317005
activated by ATPIDAPMID:16317005
contributes_toIDAPMID:16317005
GO:0004550nucleoside diphosphate kinase activityIMPPMID:62731542
GO:0005515protein bindingIPIspd1PMID:16317005831
GO:0004748ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptorISSUniProtKB:P07742GO_REF:00000242
ISOSGD:S000001328PMID:8479429
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0046704CDP metabolic processIDAPMID:163170053
GO:0006260DNA replicationIEA UniPathway:UPA00326GO_REF:0000041133
GO:0006240dCDP biosynthetic processIDAPMID:163170053
GO:0006235dTTP biosynthetic processIMPPMID:62731543
GO:0009263deoxyribonucleotide biosynthetic processISSUniProtKB:P07742GO_REF:00000247
GO:0006165nucleoside diphosphate phosphorylationIMPPMID:62731542
GO:0009216purine deoxyribonucleoside triphosphate biosynthetic processICGO:0004748GO_REF:00000012
GO:0042278purine nucleoside metabolic processICGO:0004748GO_REF:0000001163
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005737cytoplasmIDAPMID:168233724205
GO:0005829cytosolIDAPMID:168233722315
GO:0005971ribonucleoside-diphosphate reductase complexISOSGD:S000001328PMID:84794292
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopycdc22ΔNullPECO:0000005, PECO:0000137PMID:236978061338
Microscopycdc22ΔNullPMID:20473289
Cell growth assaycdc22::ura4+ (disruption)NullPMID:8479429

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopycdc22ΔNullPECO:0000005, PECO:0000137PMID:23697806686
FYPO:0001430abnormal mitotic cell cycle arrest with unreplicated DNASubstance quantification evidencecdc22-M45Not specifiedPECO:0000004PMID:627315441
FYPO:0003485abolished DNA synthesisSubstance quantification evidencecdc22-M45Not specifiedPECO:0000004PMID:69434082
Othercdc22-M45Not specifiedPECO:0000004PMID:6943408
FYPO:0000474abolished meiosisMicroscopycdc22-M45Not specifiedPMID:1088887118
FYPO:0002044abolished premeiotic DNA replicationFlow cytometry datacdc22-M45Not specifiedPMID:1088887114
FYPO:0000583abolished sporulationCell growth assaycdc22-M45PECO:0000004PMID:193412651
Cell growth assaycdc22-M45PECO:0000005PMID:1934126
FYPO:0002676dTTP absent from cellSubstance quantification evidencecdc22-M45Not specifiedPECO:0000004PMID:62731541
FYPO:0000950elongated aseptate cellMicroscopycdc22-M45Not specifiedPECO:0000004PMID:776899546
FYPO:0002675increased cellular dTDP levelSubstance quantification evidencecdc22-M45Not specifiedPECO:0000004PMID:62731541
FYPO:0001327increased protein level during vegetative growth49
affecting cig2Western blot assaycdc22-M45Not specifiedPMID:8657126
FYPO:0000314inviable after spore germination with elongated germ tube163
penetrance FYPO_EXT:0000001Microscopycdc22ΔNullPECO:0000005, PECO:0000137PMID:23697806
FYPO:0002911inviable after spore germination with swollen elongated germ tube6
penetrance FYPO_EXT:0000002Microscopycdc22::ura4+ (disruption)NullPMID:8479429
FYPO:0000839inviable elongated mononucleate aseptate cellMicroscopycdc22-M45Not specifiedPECO:0000004PMID:694340831
FYPO:0002151inviable spore465
penetrance FYPO_EXT:0000002Microscopycdc22::ura4+ (disruption)NullPMID:8479429
FYPO:0000838normal protein localization to nucleus32
affecting mcm2Microscopycdc22-M45Not specifiedPECO:0000004PMID:10588642
affecting mcm5Microscopycdc22-M45EndogenousPECO:0000004PMID:8838655
affecting mcm2Microscopycdc22-M45EndogenousPECO:0000004PMID:8838655
Target Of
OntologyRelationshipGeneProductReference
FYPO affected by mutation in res1 MBF transcription factor complex subunit Res1 PMID:7739540
FYPO affected by mutation in yox1 MBF complex corepressor Yox1 PMID:24006488
GO regulated by cdc10 MBF transcription factor complex subunit Cdc10 PMID:14648198
GO regulated by rep1 MBF transcription factor activator Rep1 PMID:14648198
GO regulated by rep2 MBF transcription factor activator Rep2 PMID:14648198
GO regulated by spd1 ribonucleotide reductase (RNR) inhibitor PMID:16317005
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
142269584227000
242273174229989

UTRs

Region Coordinates Reference
five_prime_UTR4226958..4226981PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02867 Pfam IPR000788 Ribonucleotide reductase large subunit, C-terminal 215 738 1
PF00317 Pfam IPR013509 Ribonucleotide reductase large subunit, N-terminal 143 212 1
PF03477 Pfam IPR005144 ATP-cone 1 89 1
PS51161 Prosite Profiles IPR005144 ATP-cone 1 92 1
PS00089 Prosite Patterns IPR000788 Ribonucleotide reductase large subunit, C-terminal 580 602 1
PTHR11573 HMMPANTHER 1 810 1
PTHR11573:SF6 HMMPANTHER 1 810 1
3.20.70.20 Gene3D 591 636 1
3.20.70.20 Gene3D 389 546 1
3.20.70.20 Gene3D 149 339 1
SSF48168 SuperFamily IPR008926 Ribonucleotide reductase R1 subunit, N-terminal 6 214 1
SSF51998 SuperFamily 674 750 1
SSF51998 SuperFamily 215 641 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 526 549 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 460 483 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 498 520 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 423 434 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 591 618 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 293 312 1
TIGR02506 tigrfam IPR013346 Ribonucleotide reductase, class I , alpha subunit 146 740 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.44 Da
Charge -0.50
Isoelectric point 6.47
Molecular weight 92.00 kDa
Number of residues 811
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelabsent during GO:0000084Northern assay evidencePMID:8313888
increased in presence of hydroxyurea Northern assay evidencePMID:8479429
present during GO:0000080Northern assay evidencePMID:8313888
present during GO:0000082Northern assay evidencePMID:8313888
absent during GO:0000087Northern assay evidencePMID:8313888
absent during GO:0000085Northern assay evidencePMID:8313888
absent during GO:0000086Northern assay evidencePMID:8313888
increased in presence of 4-nitroquinoline N-oxide Northern assay evidencePMID:8842148
increased during GO:0072711Northern assay evidencePMID:24006488
present during GO:0072690Northern assay evidencePMID:24006488

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
64842during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
88231during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
49618during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
55635during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
35764.13during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
79898during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
3877.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
16during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
2.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
promoter contains MCB PMID:146481984
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400
conserved in archaea237
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
cdc45DNA replication pre-initiation complex subunit Cdc45 Synthetic Growth DefectPMID:11523776
rad4BRCT domain protein Rad4 Synthetic LethalityPMID:9268024
hus5SUMO conjugating enzyme Hus5 Synthetic Growth DefectPMID:7768995
mcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
ddb1damaged DNA binding protein Ddb1 Synthetic RescuePMID:24652833
Phenotypic Suppression
chk1Chk1 protein kinase Phenotypic EnhancementPMID:10683155
spd1ribonucleotide reductase (RNR) inhibitor Synthetic LethalityPMID:23986475
cut14condensin complex subunit Cut14 Synthetic LethalityPMID:22645654
liz1pantothenate transporter Liz1 Phenotypic EnhancementPMID:9950674
mcm4MCM complex subunit Mcm4/Cdc21 Phenotypic EnhancementPMID:18753627
atp10F1-F0 ATPase assembly protein (predicted) Negative GeneticPMID:22681890
rad17RFC related checkpoint protein Rad17 Phenotypic EnhancementPMID:10683155
Synthetic Lethality
rad31SUMO E1-like activator enzyme Rad31 Phenotypic EnhancementPMID:9092625
SPBC29A10.16ccytochrome b5 (predicted) Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
nts1Schizosaccharomyces specific protein Affinity Capture-MSPMID:25002536
fic1C2 domain protein Fic1 Affinity Capture-MSPMID:19139265
cyk3cytokinesis protein Cyk3 (predicted) Affinity Capture-MSPMID:20603077
rng2IQGAP Affinity Capture-MSPMID:20603077
rad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:20231270
spd1ribonucleotide reductase (RNR) inhibitor Reconstituted ComplexPMID:16317005
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
suc22ribonucleotide reductase small subunit Suc22 FRETPMID:24652833
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPAC1F7.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1F7.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1F7.05 BioGRID Interaction Datasets
Expression Viewer SPAC1F7.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1F7.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1F7.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1F7.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1F7.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1F7.05 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1F7.05 Cell Cycle Data
GEO SPAC1F7.05 GEO profiles
PInt SPAC1F7.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1F7.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1F7.05 Fission yeast phenotypic data & analysis
SPD / RIKEN47/47B09Orfeome Localization Data
IntEnz1.17.4.1Integrated relational Enzyme database
Rhea1.17.4.1Annotated reactions database
UniProtKB/SwissProtP36602Ribonucleoside-diphosphate reductase large chain
ModBaseP36602Database of comparative protein structure models
STRINGP36602Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594491ribonucleoside reductase large subunit Cdc22
RefSeq mRNANM_001019920972h- ribonucleoside reductase large subunit Cdc22 (cdc22), mRNA
European Nucleotide ArchiveCAA46232.1ENA Protein Mapping
European Nucleotide ArchiveCAA91952.1ENA Protein Mapping
UniParcUPI000013396DUniProt Archive

Literature for cdc22

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014