cdc22 (SPAC1F7.05)


Gene Standard Namecdc22 Characterisation Statuspublished
Systematic IDSPAC1F7.05 Feature Typeprotein coding
Synonyms Name Description
Productribonucleoside reductase large subunit Cdc22 Product Size811aa, 92.00 kDa
Genomic Location Chromosome I, 4226958-4229989 (3032nt); CDS:4226982-4229733 (2752nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding558
Annotation ExtensionEvidenceWith/FromReference
CDP reductase activity2
Annotation ExtensionEvidenceWith/FromReference
nucleoside diphosphate kinase activity2
Annotation ExtensionEvidenceWith/FromReference
protein binding859
Annotation ExtensionEvidenceWith/FromReference
ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
CDP metabolic process3
Annotation ExtensionEvidenceWith/FromReference
dCDP biosynthetic process3
Annotation ExtensionEvidenceWith/FromReference
deoxyribonucleotide biosynthetic process7
Annotation ExtensionEvidenceWith/FromReference
dTTP biosynthetic process3
Annotation ExtensionEvidenceWith/FromReference
nucleoside diphosphate phosphorylation2
Annotation ExtensionEvidenceWith/FromReference
purine deoxyribonucleoside triphosphate biosynthetic process2
Annotation ExtensionEvidenceWith/FromReference
purine nucleoside metabolic process99
Annotation ExtensionEvidenceWith/FromReference
pyrimidine deoxyribonucleoside triphosphate biosynthetic process4
Annotation ExtensionEvidenceWith/FromReference
pyrimidine nucleoside metabolic process20
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4209
Annotation ExtensionEvidenceWith/FromReference
cytosol2314
Annotation ExtensionEvidenceWith/FromReference
ribonucleoside-diphosphate reductase complex2
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Inviable

Population Phenotype

Term NameAlleleExpressionCount
inviable vegetative cell populationcdc22::ura4+ (disruption)Null1438
cdc22ΔNull

Cell Phenotype

Term NameAlleleExpressionCount
abnormal mitotic cell cyclecdc22ΔNull834
abnormal mitotic cell cycle arrest with unreplicated DNAcdc22-M45Not specified50
abolished DNA synthesiscdc22-M45Not specified1
abolished meiosiscdc22-M45Not specified17
abolished premeiotic DNA replicationcdc22-M45Not specified14
abolished sporulationcdc22-M4554
dTTP absent from cellcdc22-M45Not specified1
elongated aseptate cellcdc22-M45Not specified57
increased cellular dTDP levelcdc22-M45Not specified1
increased protein level during vegetative growth83
affecting cig2cdc22-M45Not specified
inviable after spore germination with elongated germ tubecdc22ΔNull164
inviable after spore germination, without cell division, with swollen elongated germ tubecdc22::ura4+ (disruption)Null7
inviable elongated mononucleate aseptate cellcdc22-M45Not specified34
inviable sporecdc22::ura4+ (disruption)Null472
normal protein localization to nucleus55
affecting mcm5cdc22-M45Endogenous
affecting mcm2cdc22-M45Not specified
affecting mcm2cdc22-M45Endogenous
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in res1 MBF transcription factor complex subunit Res1
FYPO affected by mutation in yox1 MBF complex corepressor Yox1
GO regulated by cdc10 MBF transcription factor complex subunit Cdc10
GO regulated by rep1 MBF transcription factor activator Rep1
GO regulated by rep2 MBF transcription factor activator Rep2
GO regulated by spd1 ribonucleotide reductase (RNR) inhibitor
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4226958..4227000, 4227317..4229989
mRNA4226958..4229989
5' UTR4226958..4226981PMID:21511999
CDS4226982..4227000, 4227317..4229733
3' UTR4229734..4229989SPC05253
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03477 Pfam IPR005144 ATP-cone 1 89 1
PF02867 Pfam IPR000788 Ribonucleotide reductase large subunit, C-terminal 215 738 1
PF00317 Pfam IPR013509 Ribonucleotide reductase large subunit, N-terminal 143 212 1
PS51161 Prosite Profiles IPR005144 ATP-cone 1 92 1
PS00089 Prosite Patterns IPR000788 Ribonucleotide reductase large subunit, C-terminal 580 602 1
PTHR11573:SF6 HMMPANTHER 1 810 1
PTHR11573 HMMPANTHER 1 810 1
3.20.70.20 Gene3D 591 636 1
3.20.70.20 Gene3D 389 546 1
3.20.70.20 Gene3D 149 339 1
SSF51998 SuperFamily 674 750 1
SSF51998 SuperFamily 215 641 1
SSF48168 SuperFamily IPR008926 Ribonucleotide reductase R1 subunit, N-terminal 6 214 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 293 312 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 460 483 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 498 520 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 423 434 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 591 618 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 526 549 1
TIGR02506 tigrfam IPR013346 Ribonucleotide reductase, class I , alpha subunit 146 740 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.44 Da
Charge -0.50
Isoelectric point 6.47
Molecular weight 92.00 kDa
Number of residues 811
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelabsent during GO:0000087Northern assay evidencePMID:8313888
increased in presence of hydroxyurea Northern assay evidencePMID:8479429
absent during GO:0000084Northern assay evidencePMID:8313888
absent during GO:0000086Northern assay evidencePMID:8313888
absent during GO:0000085Northern assay evidencePMID:8313888
present during GO:0072690Northern assay evidencePMID:24006488
increased during GO:0072711Northern assay evidencePMID:24006488
increased in presence of 4-nitroquinoline N-oxide Northern assay evidencePMID:8842148
present during GO:0000082Northern assay evidencePMID:8313888
present during GO:0000080Northern assay evidencePMID:8313888

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
64842during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
88231during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
49618during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
55635during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
35764.13during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
79898during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
3877.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
16during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
2.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
promoter contains MCB PMID:146481984
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in bacteria1001
conserved in metazoa3424
conserved in vertebrates3399
conserved in archaea238
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured bycyk3cytokinesis protein Cyk3 (predicted) Affinity Capture-MSPMID:20603077
affinity captured byfic1C2 domain protein Fic1 Affinity Capture-MSPMID:19139265
affinity captured bynts1Clr6 histone deacetylase complex subunit Nts1 Affinity Capture-MSPMID:25002536
affinity captured byrad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:20231270
affinity captured byrng2IQGAP Affinity Capture-MSPMID:20603077
forms complex withspd1ribonucleotide reductase (RNR) inhibitor Reconstituted ComplexPMID:16317005
forms complex withspd1ribonucleotide reductase (RNR) inhibitor Reconstituted ComplexPMID:16317005
fluorescence resonance energy acceptor fromsuc22ribonucleotide reductase small subunit Suc22 FRETPMID:24652833
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withatp10mitochondrial F1-F0 ATPase assembly protein (predicted) Negative GeneticPMID:22681890
synthetic growth defect withcdc45DNA replication pre-initiation complex subunit Cdc45 Synthetic Growth DefectPMID:11523776
enhances phenotype ofchk1Chk1 protein kinase Phenotypic EnhancementPMID:10683155
synthetic lethal withcut14condensin complex subunit Cut14 Synthetic LethalityPMID:22645654
rescuesddb1damaged DNA binding protein Ddb1 Phenotypic SuppressionPMID:24652833
synthetically rescuesddb1damaged DNA binding protein Ddb1 Synthetic RescuePMID:24652833
synthetic growth defect withhus5SUMO conjugating enzyme E2 Hus5 Synthetic Growth DefectPMID:7768995
enhances phenotype ofliz1pantothenate transmembrane transporter Liz1 Phenotypic EnhancementPMID:9950674
synthetic growth defect withmcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
phenotype enhanced bymcm4MCM complex subunit Mcm4/Cdc21 Phenotypic EnhancementPMID:18753627
rescuespol1DNA polymerase alpha catalytic subunit Phenotypic SuppressionPMID:9693370
enhances phenotype ofrad17RFC related checkpoint protein Rad17 Phenotypic EnhancementPMID:10683155
synthetic lethal withrad17RFC related checkpoint protein Rad17 Synthetic LethalityPMID:10683155
enhances phenotype ofrad31SUMO activating enzyme E1-type Rad31 Phenotypic EnhancementPMID:9092625
synthetic lethal withrad4BRCT domain protein Rad4 Synthetic LethalityPMID:9268024
negative genetic interaction withSPBC29A10.16ccytochrome b5 (predicted) Negative GeneticPMID:22681890
synthetic lethal withspd1ribonucleotide reductase (RNR) inhibitor Synthetic LethalityPMID:23986475
External References
Database Identifier Description
NBRP SPAC1F7.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1F7.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1F7.05 BioGRID Interaction Datasets
Expression Viewer SPAC1F7.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1F7.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1F7.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1F7.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1F7.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1F7.05 Transcriptome Viewer (Bähler Lab)
GEO SPAC1F7.05 GEO profiles
PInt SPAC1F7.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1F7.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1F7.05 Fission yeast phenotypic data & analysis
Cyclebase SPAC1F7.05.1 Cell Cycle Data
SPD / RIKEN47/47B09Orfeome Localization Data
IntEnz1.17.4.1Integrated relational Enzyme database
Rhea1.17.4.1Annotated reactions database
UniProtKB/SwissProtP36602Ribonucleoside-diphosphate reductase large chain
ModBaseP36602Database of comparative protein structure models
STRINGP36602Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594491ribonucleoside reductase large subunit Cdc22
RefSeq mRNANM_001019920972h- ribonucleoside reductase large subunit Cdc22 (cdc22), mRNA
European Nucleotide ArchiveCAA46232.1ENA Protein Mapping
European Nucleotide ArchiveCAA91952.1ENA Protein Mapping
MetaCycPWY-6545Pyrimidine deoxyribonucleotides de novo biosynthesis III
MetaCycPWY-7184Pyrimidine deoxyribonucleotides de novo biosynthesis I
MetaCycPWY-7198Pyrimidine deoxyribonucleotides de novo biosynthesis IV
MetaCycPWY-7210Pyrimidine deoxyribonucleotides biosynthesis from CTP
MetaCycPWY-7220Adenosine deoxyribonucleotides de novo biosynthesis II
MetaCycPWY-7222Guanosine deoxyribonucleotides de novo biosynthesis II
MetaCycPWY-7226Guanosine deoxyribonucleotides de novo biosynthesis I
MetaCycPWY-7227Adenosine deoxyribonucleotides de novo biosynthesis I
KEGG_Enzyme00230+1.17.4.1Purine metabolism
KEGG_Enzyme00240+1.17.4.1Pyrimidine metabolism
KEGG_Enzyme00480+1.17.4.1Glutathione metabolism
UniParcUPI000013396DUniProt Archive
UniPathwayUPA00326Genetic information processing; DNA replication

Literature for cdc22

Search: Europe PMC or PubMed

Release Version: PomBase:26_52 - 11 May 2015