cdc22 (SPAC1F7.05)


Gene Standard Namecdc22 Characterisation Statuspublished
Systematic IDSPAC1F7.05 Feature Typeprotein coding
Synonyms Name Description
Productribonucleoside reductase large subunit Cdc22 Product Size811aa, 92.00 kDa
Genomic Location Chromosome I, 4226958-4229989 (3032nt); CDS:4226982-4229733 (2752nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
ATP binding557
Annotation ExtensionEvidenceWith/FromReference
CDP reductase activity2
Annotation ExtensionEvidenceWith/FromReference
nucleoside diphosphate kinase activity2
Annotation ExtensionEvidenceWith/FromReference
protein binding887
Annotation ExtensionEvidenceWith/FromReference
ribonucleoside-diphosphate reductase activity2
Annotation ExtensionEvidenceWith/FromReference
ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor2
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
CDP metabolic process3
Annotation ExtensionEvidenceWith/FromReference
dCDP biosynthetic process3
Annotation ExtensionEvidenceWith/FromReference
deoxyribonucleotide biosynthetic process7
Annotation ExtensionEvidenceWith/FromReference
dTTP biosynthetic process3
Annotation ExtensionEvidenceWith/FromReference
nucleoside diphosphate phosphorylation16
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
ribonucleoside-diphosphate reductase complex2
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Inviable

Population Phenotype

Term NameGenotypesCount
abnormal mitotic cell cycle arrest with unreplicated DNAcdc22-M4557
inviable vegetative cell populationcdc22::ura4+1455
cdc22Δ

Cell Phenotype

Term NameGenotypesCount
abnormal mitotic cell cyclecdc22Δ894
abolished DNA synthesiscdc22-M451
abolished meiosiscdc22-M4520
abolished premeiotic DNA replicationcdc22-M4515
abolished sporulationcdc22-M4556
cutcdc22-M45128
decreased chromatin binding12
affecting rad17cdc22-M45
decreased protein phosphorylation during vegetative growth104
affecting pol1cdc22-M45
dTTP absent from cellcdc22-M451
elongated aseptate vegetative cellcdc22-M4572
increased cellular dTDP levelcdc22-M451
increased duration of horsetail movementcdc22-M454
increased number of double-strand break sites during vegetative growthcdc22-M4519
increased number of Rad52 foci during vegetative growthcdc22-M4545
increased protein level during vegetative growth114
affecting cig2cdc22-M45
inviable after spore germination with elongated germ tubecdc22Δ164
inviable after spore germination, without cell division, with swollen elongated germ tubecdc22::ura4+7
inviable elongated mononucleate aseptate cellcdc22-M4544
inviable sporecdc22::ura4+476
normal protein localization to nucleus during vegetative growth83
affecting mcm5cdc22-M45
affecting mcm2cdc22-M45
normal protein phosphorylation during vegetative growth96
affecting pol1cdc22-M45

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased vegetative cell population growthmcm4-c84 (828-911), cdc22-M45
inviable vegetative cell populationcdc22-M45, cdc2-3w (C67Y)
cnd2-1, cdc22-c11

Cell Phenotype

Term NameGenotypes
abnormal mitotic cell cycle regulation during cellular response to hydroxyureacdc22-M45, cdc2-3w (C67Y)
cutcdc22-M45, cds1::ura4
cdc22-M45, hsk1-89 (L337P, E403K, L416P)
inviable small vegetative cellcdc22-M45, cdc2-3w (C67Y)
normal duration of horsetail movementcdc22-M45, cds1Δ
septation following abnormal chromosome segregation, with binucleate and anucleate compartment formation during cellular response to hydroxyureacdc22-M45, cdc2-3w (C67Y)
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in cdc10 MBF transcription factor complex subunit Cdc10
FYPO affected by mutation in cds1 replication checkpoint kinase Cds1
FYPO affected by mutation in gad8 AGC family protein kinase Gad8
FYPO affected by mutation in nrm1 MBF complex corepressor Nrm1
FYPO affected by mutation in res1 MBF transcription factor complex subunit Res1
FYPO affected by mutation in tor1 serine/threonine protein kinase Tor1
FYPO affected by mutation in yox1 MBF complex corepressor Yox1
GO regulated by cdc10 MBF transcription factor complex subunit Cdc10
GO regulated by nrm1 MBF complex corepressor Nrm1
GO regulated by rep1 MBF transcription factor activator Rep1
GO regulated by rep2 MBF transcription factor activator Rep2
GO regulated by spd1 ribonucleotide reductase (RNR) inhibitor
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4226958..4227000, 4227317..4229989
Intron4227001..4227316
mRNA4226958..4229989
5' UTR4226958..4226981PMID:21511999
CDS4226982..4227000, 4227317..4229733
3' UTR4229734..4229989SPC05253
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00317 Pfam IPR013509 Ribonucleotide reductase large subunit, N-terminal 143 212 1
PF03477 Pfam IPR005144 ATP-cone domain 1 89 1
PF02867 Pfam IPR000788 Ribonucleotide reductase large subunit, C-terminal 216 737 1
PS51161 Prosite Profiles IPR005144 ATP-cone domain 1 92 1
PS00089 Prosite Patterns IPR000788 Ribonucleotide reductase large subunit, C-terminal 580 602 1
PTHR11573 HMMPANTHER 2 766 1
PTHR11573 HMMPANTHER 785 810 1
3.20.70.20 Gene3D Ribonucleotide reductase large subunit, C-terminal 389 546 1
3.20.70.20 Gene3D Ribonucleotide reductase large subunit, C-terminal 149 339 1
3.20.70.20 Gene3D Ribonucleotide reductase large subunit, C-terminal 591 636 1
SSF51998 SuperFamily 674 750 1
SSF51998 SuperFamily 215 641 1
SSF48168 SuperFamily IPR008926 Ribonucleotide reductase R1 subunit, N-terminal 6 214 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 591 618 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 498 520 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 460 483 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 526 549 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 423 434 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 293 312 1
TIGR02506 TIGRFAM IPR013346 Ribonucleotide reductase, class I , alpha subunit 146 740 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.44 Da
Charge -0.50
Codon Adaptation Index 0.63
Isoelectric point 6.47
Molecular weight 92.00 kDa
Number of residues 811
Modifications

Protein Modifications

Term NameResidueCount
O-phospho-L-serineS810 2289
Annotation ExtensionEvidenceResidueReference
IDA S810 PMID:25720772
ubiquitinylated lysineK376 512
Annotation ExtensionEvidenceResidueReference
IDA PMID:26412298
mass spectrometry evidence K376 PMID:26412298
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelabsent during GO:0000085Northern assay evidencePMID:8313888
absent during GO:0000085Northern assay evidencePMID:12898217
absent during GO:0000084Northern assay evidencePMID:12898217
increased during GO:0072711Northern assay evidencePMID:24006488
absent during GO:0000086Northern assay evidencePMID:12898217
present during GO:0000080Northern assay evidencePMID:8313888
present during GO:0000082Northern assay evidencePMID:8313888
absent during GO:0000087Northern assay evidencePMID:12898217
absent during GO:0000086Northern assay evidencePMID:8313888
increased in presence of 4-nitroquinoline N-oxide Northern assay evidencePMID:8842148
absent during GO:0000084Northern assay evidencePMID:8313888
absent during GO:0000087Northern assay evidencePMID:8313888
increased in presence of hydroxyurea Northern assay evidencePMID:8479429
present during GO:0072690Northern assay evidencePMID:24006488
increased during GO:0072711quantitative PCRPMID:26912660

Quantitative Gene Expression

View graphical display of gene expression data for cdc22 (SPAC1F7.05)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
64842during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
88231during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
49618during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
55635during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
79898during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
35764.13during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
3877.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
16during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
2.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Misc
DescriptionQualifierReferenceCount
promoter contains MCB PMID:146481984
cdc22 transcription start sites at -267 and -275 PMID:128982171
two MCB clusters regulate cell-cycle dependent cdc22+ transcription PMID:128982171
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3093
conserved in fungi4607
conserved in eukaryotes4517
conserved in bacteria1005
conserved in metazoa3427
conserved in vertebrates3402
conserved in archaea242
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Load genes that interact physically with SPAC1F7.05 into the Query Builder
View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byrng2IQGAP Affinity Capture-MSPMID:20603077
affinity captured bycbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
affinity captured bytea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
affinity captured bycyk3cytokinesis protein Cyk3 (predicted) Affinity Capture-MSPMID:20603077
affinity captured byrad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:20231270
affinity captured byfic1C2 domain protein Fic1 Affinity Capture-MSPMID:19139265
affinity captured bynts1Clr6 histone deacetylase complex subunit Nts1 Affinity Capture-MSPMID:25002536
fluorescence resonance energy acceptor fromsuc22ribonucleotide reductase small subunit Suc22 FRETPMID:24652833
forms complex withspd1ribonucleotide reductase (RNR) inhibitor Reconstituted ComplexPMID:16317005
Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC1F7.05 into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
enhances phenotype ofcds1replication checkpoint kinase Cds1 Phenotypic EnhancementPMID:7723827
enhances phenotype ofliz1pantothenate transmembrane transporter Liz1 Phenotypic EnhancementPMID:9950674
enhances phenotype ofrad17RFC related checkpoint protein Rad17 Phenotypic EnhancementPMID:10683155
enhances phenotype ofchk1Chk1 protein kinase Phenotypic EnhancementPMID:10683155
enhances phenotype ofrad31SUMO activating enzyme E1-type Rad31 Phenotypic EnhancementPMID:9092625
synthetically rescued bycds1replication checkpoint kinase Cds1 Synthetic RescuePMID:25492408
synthetic lethal withcnd2condensin complex non-SMC subunit Cnd2 Synthetic LethalityPMID:12000964
synthetic lethal withcut14condensin complex SMC subunit Smc2 Synthetic LethalityPMID:22645654
synthetic lethal withrad17RFC related checkpoint protein Rad17 Synthetic LethalityPMID:10683155
synthetic lethal withspd1ribonucleotide reductase (RNR) inhibitor Synthetic LethalityPMID:23986475
synthetic lethal withrad4BRCT domain protein Rad4 Synthetic LethalityPMID:9268024
rescuespol1DNA polymerase alpha catalytic subunit Phenotypic SuppressionPMID:9693370
rescuesddb1damaged DNA binding protein Ddb1 Phenotypic SuppressionPMID:24652833
synthetically rescuesddb1damaged DNA binding protein Ddb1 Synthetic RescuePMID:24652833
synthetic growth defect withmcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
synthetic growth defect withcdc45DNA replication pre-initiation complex subunit Cdc45 Synthetic Growth DefectPMID:11523776
synthetic growth defect withhus5SUMO conjugating enzyme E2 Hus5 Synthetic Growth DefectPMID:7768995
negative genetic interaction withSPBC29A10.16ccytochrome b5 (predicted) Negative GeneticPMID:22681890
negative genetic interaction withatp10mitochondrial F1-F0 ATPase assembly protein (predicted) Negative GeneticPMID:22681890
phenotype enhanced bymcm4MCM complex subunit Mcm4/Cdc21 Phenotypic EnhancementPMID:18753627
External References
Database Identifier Description
NBRP SPAC1F7.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1F7.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1F7.05 BioGRID Interaction Datasets
Expression Viewer SPAC1F7.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1F7.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1F7.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1F7.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1F7.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1F7.05 Transcriptome Viewer (Bähler Lab)
GEO SPAC1F7.05 GEO profiles
PInt SPAC1F7.05 Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1F7.05 Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1F7.05 Fission yeast phenotypic data & analysis
Cyclebase SPAC1F7.05.1 Cell Cycle Data
IntEnz1.17.4.1Integrated relational Enzyme database
Rhea1.17.4.1Annotated reactions database
SPD / RIKEN47/47B09Orfeome Localization Data
UniProtKB/SwissProtP36602Ribonucleoside-diphosphate reductase large chain
ModBaseP36602Database of comparative protein structure models
STRINGP36602Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594491ribonucleoside reductase large subunit Cdc22
RefSeq mRNANM_001019920972h- ribonucleoside reductase large subunit Cdc22 (cdc22), mRNA
Uniprot/SPTREMBLS6BSK3Ribonucleoside-diphosphate reductase
European Nucleotide ArchiveCAA46232.1ENA Protein Mapping
European Nucleotide ArchiveCAA91952.1ENA Protein Mapping
MetaCycPWY-6545Pyrimidine deoxyribonucleotides de novo biosynthesis III
MetaCycPWY-7184Pyrimidine deoxyribonucleotides de novo biosynthesis I
MetaCycPWY-7198Pyrimidine deoxyribonucleotides de novo biosynthesis IV
MetaCycPWY-7210Pyrimidine deoxyribonucleotides biosynthesis from CTP
MetaCycPWY-7220Adenosine deoxyribonucleotides de novo biosynthesis II
MetaCycPWY-7222Guanosine deoxyribonucleotides de novo biosynthesis II
MetaCycPWY-7226Guanosine deoxyribonucleotides de novo biosynthesis I
MetaCycPWY-7227Adenosine deoxyribonucleotides de novo biosynthesis
KEGG00230+1.17.4.1Purine metabolism
KEGG00240+1.17.4.1Pyrimidine metabolism
KEGG00480+1.17.4.1Glutathione metabolism
UniParcUPI000013396DUniProt Archive

Literature for cdc22

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016