cdc22 (SPAC1F7.05)

Gene Standard Namecdc22 Characterisation Statuspublished
Systematic IDSPAC1F7.05 Feature Typeprotein coding
Synonyms Name Description
Productribonucleoside reductase large subunit Cdc22 Product Size811aa, 92.00 kDa
Genomic Location Chromosome I, 4226958-4229989 (3032nt); CDS:4226982-4229733 (2752nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005524ATP bindingIEAUniProtKB-KW:KW-0067GO_REF:0000037559
GO:0004550nucleoside diphosphate kinase activityIMPPMID:62731542
GO:0005515protein bindingIPIspd1PMID:16317005887
GO:0004748ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptorISSUniProtKB:P07742GO_REF:00000242
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0006235dTTP biosynthetic processIMPPMID:62731543
GO:0009263deoxyribonucleotide biosynthetic processISSUniProtKB:P07742GO_REF:00000245
GO:0006165nucleoside diphosphate phosphorylationIMPPMID:62731542
GO:0009216purine deoxyribonucleoside triphosphate biosynthetic processICGO:0004748GO_REF:00000012
GO:0042278purine nucleoside metabolic processICGO:0004748GO_REF:000000195
GO:0006213pyrimidine nucleoside metabolic processICGO:0004748GO_REF:000000120
GO:0090329regulation of DNA-dependent DNA replicationIMPPMID:847942926
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005971ribonucleoside-diphosphate reductase complexIEAIPR008926GO_REF:00000022
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002061inviable vegetative cell populationMicroscopycdc22ΔNullPECO:0000005, PECO:0000137PMID:236978061309

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000059abnormal mitotic cell cycleMicroscopycdc22ΔNullPECO:0000005, PECO:0000137PMID:23697806564
FYPO:0000583abolished sporulationCell growth assaycdc22-M45PECO:0000004PMID:193412625
Cell growth assaycdc22-M45PECO:0000005PMID:1934126
FYPO:0001430cell cycle arrest with unreplicated DNASubstance quantification evidencecdc22-M45Not specifiedPECO:0000004PMID:627315410
FYPO:0002676dTTP absent from cellSubstance quantification evidencecdc22-M45Not specifiedPECO:0000004PMID:62731541
FYPO:0001122elongated vegetative cellno_namePMID:6943408624
FYPO:0002675increased cellular dTDP levelSubstance quantification evidencecdc22-M45Not specifiedPECO:0000004PMID:62731541
FYPO:0000314inviable after spore germination with elongated germ tubeMicroscopycdc22ΔNullPECO:0000005, PECO:0000137PMID:23697806162
penetrance FYPO_EXT:0000001
FYPO:0000838normal protein localization to nucleusMicroscopycdc22-M45Not specifiedPECO:0000004PMID:1058864224
affecting mcm2
affecting mcm5Microscopycdc22-M45EndogenousPECO:0000004PMID:8838655
affecting mcm2Microscopycdc22-M45EndogenousPECO:0000004PMID:8838655
Target Of
regulated by cdc10 MBF transcription factor complex subunit Cdc10 PMID:14648198
regulated by rep1 MBF transcription factor complex subunit Rep1 PMID:14648198
regulated by rep2 transcriptional activator, MBF subunit Rep2 PMID:14648198
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Start End Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF03477 Pfam IPR005144 ATP-cone 1 89 1
PF02867 Pfam IPR000788 Ribonucleotide reductase large subunit, C-terminal 215 738 1
PF00317 Pfam IPR013509 Ribonucleotide reductase large subunit, N-terminal 143 212 1
PS51161 Prosite Profiles IPR005144 ATP-cone 1 92 1
PS00089 Prosite Patterns IPR000788 Ribonucleotide reductase large subunit, C-terminal 580 602 1
PTHR11573:SF6 HMMPANTHER 1 810 1
PTHR11573 HMMPANTHER 1 810 1
G3DSA: Gene3D 591 636 1
G3DSA: Gene3D 389 546 1
G3DSA: Gene3D 149 339 1
SSF48168 SuperFamily IPR008926 6 214 1
SSF51998 SuperFamily 674 750 1
SSF51998 SuperFamily 215 641 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 498 520 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 293 312 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 460 483 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 591 618 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 526 549 1
PR01183 PRINTS IPR000788 Ribonucleotide reductase large subunit, C-terminal 423 434 1
TIGR02506 tigrfam IPR013346 Ribonucleotide reductase, class I , alpha subunit 146 740 1

View domain organization at Pfam

Protein Properties

Ave. residue weight 113.44 Da
Charge -0.50
Isoelectric point 6.47
Molecular weight 92.00 kDa
Number of residues 811
Gene Expression

Qualitative Gene Expression

RNA levelpresentNorthern assay evidencePMID:24006488
during GO:0072690
increasedNorthern assay evidencePMID:24006488
during GO:0072711

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
3877.67during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
35764.13during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
16during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
2.5during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
promoter contains MCB PMID:146481984
Species Distribution
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in bacteria1001
conserved in metazoa3419
conserved in vertebrates3394
conserved in archaea237

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
cdc45DNA replication pre-initiation complex subunit Cdc45 Synthetic Growth DefectPMID:11523776
rad4BRCT domain protein Rad4 Synthetic LethalityPMID:9268024
hus5SUMO conjugating enzyme Hus5 Synthetic Growth DefectPMID:7768995
mcl1DNA polymerase alpha accessory factor Mcl1 Synthetic Growth DefectPMID:12455694
rad17RFC related checkpoint protein Rad17 Synthetic LethalityPMID:10683155
Phenotypic Enhancement
chk1Chk1 protein kinase Phenotypic EnhancementPMID:10683155
spd1ribonucleotide reductase (RNR) inhibitor Synthetic LethalityPMID:23986475
cut14condensin complex subunit Cut14 Synthetic LethalityPMID:22645654
liz1pantothenate transporter Liz1 Phenotypic EnhancementPMID:9950674
mcm4MCM complex subunit Mcm4/Cdc21 Phenotypic EnhancementPMID:18753627
atp10F1-F0 ATPase assembly protein (predicted) Negative GeneticPMID:22681890
rad31SUMO E1-like activator enzyme Rad31 Phenotypic EnhancementPMID:9092625
SPBC29A10.16ccytochrome b5 (predicted) Negative GeneticPMID:22681890
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
fic1C2 domain protein Fic1 Affinity Capture-MSPMID:19139265
cyk3cytokinesis protein Cyk3 (predicted) Affinity Capture-MSPMID:20603077
rng2IQGAP Affinity Capture-MSPMID:20603077
rad2414-3-3 protein Rad24 Affinity Capture-WesternPMID:20231270
spd1ribonucleotide reductase (RNR) inhibitor Reconstituted ComplexPMID:16317005
cbf11CBF1/Su(H)/LAG-1 family transcription factor Cbf11 Affinity Capture-MSPMID:22540037
bdf2BET family double bromodomain protein Bdf2 Affinity Capture-MSPMID:24013502
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
epe1Jmjc domain chromatin associated protein Epe1 Affinity Capture-MSPMID:24013502
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPAC1F7.05 Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1F7.05 Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1F7.05 BioGRID Interaction Datasets
Expression Viewer SPAC1F7.05 Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1F7.05 Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1F7.05 Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1F7.05 Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1F7.05 Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1F7.05 Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1F7.05 Cell Cycle Data
GEO SPAC1F7.05 GEO profiles
PInt SPAC1F7.05 Protein-Protein Interaction Predictor (Bähler Lab)
IntEnz1.17.4.1Integrated relational Enzyme database
Rhea1.17.4.1Annotated reactions database
WikiGene2541695ribonucleoside reductase large subunit Cdc22
EntrezGene2541695ribonucleoside reductase large subunit Cdc22
SPD / RIKEN47/47B09Orfeome Localization Data
UniProtKB/SwissProtP36602Ribonucleoside-diphosphate reductase large chain
ModBaseP36602Database of comparative protein structure models
StringP36602Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594491ribonucleoside reductase large subunit Cdc22
RefSeq mRNANM_001019920972h- ribonucleoside reductase large subunit Cdc22 (cdc22), mRNA
European Nucleotide ArchiveCAA46232ENA Protein Mapping
European Nucleotide ArchiveCAA91952ENA Protein Mapping
UniParcUPI000013396DUniProt Archive

Literature for cdc22

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014