rpl801 (SPAC1F7.13c)


Gene Standard Namerpl801 Characterisation Statusbiological_role_inferred
Systematic IDSPAC1F7.13c Feature Typeprotein coding
Synonymsrpk5a, rpl18, rpl2-1, rpl8-1, SPAC21E11.02c Name Description
Product60S ribosomal protein L8 (predicted) Product Size253aa, 27.10 kDa
Genomic Location Chromosome I, 4251468-4250595 (874nt); CDS:4251418-4250657 (762nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0019843rRNA bindingIEAUniProtKB-KW:KW-0699GO_REF:000003756
GO:0003735structural constituent of ribosomeISOSGD:S000001280GO_REF:0000024255
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0002181cytoplasmic translationISOSGD:S000001280GO_REF:0000024473
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005829cytosolIDAPMID:168233722315
GO:0022625cytosolic large ribosomal subunitISOSGD:S000001280GO_REF:000002483
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyrpl801ΔNullPECO:0000005, PECO:0000137PMID:236978063759
Microscopyrpl801ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003625abnormal microtubule cytoskeleton morphology during mitotic interphase186
penetrance FYPO_EXT:0000002experimental phenotypic evidencerpl801ΔNullPECO:0000005, PECO:0000103, PECO:0000137DOI:10.1016/j.devcel.2014.09.005
FYPO:0002736abnormal mitotic cell cycle phase103
penetrance FYPO_EXT:0000002experimental phenotypic evidencerpl801ΔNullPECO:0000005, PECO:0000103, PECO:0000137DOI:10.1016/j.devcel.2014.09.005
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyrpl801ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
142514684250595

UTRs

Region Coordinates Reference
five_prime_UTR4251468..4251419PMID:21511999
three_prime_UTR4250656..4250595PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF00181 Pfam IPR022666 Ribosomal Proteins L2, RNA binding domain 15 89 4
PF03947 Pfam IPR022669 Ribosomal protein L2, C-terminal 96 228 4
PS00467 Prosite Patterns IPR022671 Ribosomal protein L2, conserved site 196 207 4
PTHR13691 HMMPANTHER IPR002171 Ribosomal protein L2 1 253 4
PTHR13691:SF4 HMMPANTHER 1 253 3
2.40.50.140 Gene3D IPR012340 Nucleic acid-binding, OB-fold 1 85 48
4.10.950.10 Gene3D IPR014726 Ribosomal protein L2, domain 3 169 243 4
2.30.30.30 Gene3D IPR014722 Ribosomal protein L2 domain 2 89 168 13
SSF50249 SuperFamily IPR012340 Nucleic acid-binding, OB-fold 2 96 57
SSF50104 SuperFamily IPR008991 Translation protein SH3-like domain 98 244 16
PIRSF002158 PIRSF IPR002171 Ribosomal protein L2 1 253 3

View domain organization at Pfam

Protein Properties

Ave. residue weight 107.12 Da
Charge 29.50
Isoelectric point 11.45
Molecular weight 27.10 kDa
Number of residues 253
Modifications

Protein Modifications

Term IDTerm NameEvidenceResidueReferenceCount
MOD:00046O-phospho-L-serineexperimental evidenceS11PMID:217125471670
present during mitotic M phase
MOD:00696phosphorylated residueNASPMID:195477441922
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
307727during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
301113during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
313744during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
315965during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
374427.3during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
308773during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
39428.93during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
110during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
7.2during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in bacteria1000
conserved in metazoa3425
conserved in vertebrates3400
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
cid14poly(A) polymerase Cid14 Affinity Capture-MSPMID:20403971
air1zinc knuckle TRAMP complex subunit Air1 Affinity Capture-MSPMID:20403971
sog2leucine-rich repeat protein Lrp1 Affinity Capture-MSPMID:23462181
cwf10U5 snRNP GTPase subunit Cwf10 Affinity Capture-MSPMID:24442611
hhp1serine/threonine protein kinase Hhp1 Affinity Capture-MSPMID:24055157
tea1cell end marker Tea1 Affinity Capture-MSPMID:21652630
lea1U2 snRNP-associated protein Lea1 (predicted) Affinity Capture-MSPMID:24442611
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPAC1F7.13c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC1F7.13c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC1F7.13c BioGRID Interaction Datasets
Expression Viewer SPAC1F7.13c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC1F7.13c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC1F7.13c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC1F7.13c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC1F7.13c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC1F7.13c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC1F7.13c Cell Cycle Data
GEO SPAC1F7.13c GEO profiles
PInt SPAC1F7.13c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC1F7.13c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC1F7.13c Fission yeast phenotypic data & analysis
SPD / RIKEN40/40A11Orfeome Localization Data
UniProtKB/SwissProtP0809360S ribosomal protein L2
ModBaseP08093Database of comparative protein structure models
STRINGP08093Network display of known and predicted interactions and functional associations
RefSeq mRNAXM_001713050972h- 60S ribosomal protein L2 (rpl801), mRNA
RefSeq PeptideXP_00171310260S ribosomal protein L2
European Nucleotide ArchiveBAA87149.1ENA Protein Mapping
European Nucleotide ArchiveCAA28710.1ENA Protein Mapping
European Nucleotide ArchiveCAA34428.1ENA Protein Mapping
European Nucleotide ArchiveCAA35971.1ENA Protein Mapping
European Nucleotide ArchiveCAA91960.1ENA Protein Mapping
European Nucleotide ArchiveCAB10155.1ENA Protein Mapping
European Nucleotide ArchiveCAB46697.1ENA Protein Mapping
UniParcUPI0000133ED3UniProt Archive

Literature for rpl801

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014