fus1 (SPAC20G4.02c)


Gene Standard Namefus1 Characterisation Statuspublished
Systematic IDSPAC20G4.02c Feature Typeprotein coding
Synonyms Name Description
Productformin Fus1 Product Size1372aa, 157.07 kDa
Genomic Location Chromosome I, 4817790-4813111 (4680nt); CDS:4817343-4813225 (4119nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding37
Annotation ExtensionEvidenceWith/FromReference
Rho GTPase binding4
Annotation ExtensionEvidenceWith/FromReference
SH3 domain binding1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin cytoskeleton organization97
Annotation ExtensionEvidenceWith/FromReference
actin fusion focus assembly1
Annotation ExtensionEvidenceWith/FromReference
plasma membrane fusion involved in cytogamy6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
actin fusion focus13
Annotation ExtensionEvidenceWith/FromReference
cell division site317
Annotation ExtensionEvidenceWith/FromReference
cytoplasm4213
Annotation ExtensionEvidenceWith/FromReference
cytosol2302
Annotation ExtensionEvidenceWith/FromReference
mating projection28
Annotation ExtensionEvidenceWith/FromReference
mating projection tip27
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased mating efficiencyTR1 and TR2 deletion (deletion of both TR boxes in promoter)275
normal mating efficiencyTR2 deleted (TR2 promoter box at -238 deleted)30
TR1 deleted (TR1 promoter box at -157 deleted)
viable vegetative cell populationfus1Δ3850

Cell Phenotype

Term NameGenotypesCount
abnormal protein localization461
affects localization of dni1fus1Δ
abolished actin fusion focus assemblyfus1Δ1
abolished cytogamyfus1-B202
abolished premeiotic DNA replicationfus1-B2015
mislocalized nucleus during matingfus1-B201
normal agglutinationfus1-B202
normal shmoo formationfus1-B203
normal sporulationfus1-B20/fus1-B20 (homozygous diploid)53
viable vegetative cell with normal cell morphologyfus1Δ3102
Target Of
OntologyRelationshipGeneProduct
FYPO affected by mutation in byr1 MAP kinase kinase Byr1
FYPO affected by mutation in byr2 MAP kinase kinase kinase Byr2
FYPO affected by mutation in ste11 transcription factor Ste11
FYPO localization affected by mutation in myo51 myosin type V
FYPO localization affected by mutation in myo52 myosin type V
GO regulated by ste11 transcription factor Ste11
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4817790..4813111
mRNA4817790..4813111
5' UTR4817790..4817344PMID:21511999
CDS4817343..4813225
3' UTR4813224..4813111PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF02181 Pfam IPR015425 Formin, FH2 domain 870 1253 3
PF06371 Pfam IPR010473 Formin, GTPase-binding domain 108 296 3
SM01140 SMART IPR010473 Formin, GTPase-binding domain 103 296 4
SM00498 SMART IPR015425 Formin, FH2 domain 868 1328 3
PS51444 Prosite Profiles IPR015425 Formin, FH2 domain 868 1278 3
PS51232 Prosite Profiles IPR014768 Rho GTPase-binding/formin homology 3 (GBD/FH3) domain 104 522 3
PTHR23213:SF221 HMMPANTHER 193 432 2
PTHR23213:SF221 HMMPANTHER 469 618 2
PTHR23213:SF221 HMMPANTHER 871 1310 2
PTHR23213:SF221 HMMPANTHER 101 158 2
PTHR23213:SF221 HMMPANTHER 780 832 2
PTHR23213 HMMPANTHER 193 432 3
PTHR23213 HMMPANTHER 469 618 3
PTHR23213 HMMPANTHER 871 1310 3
PTHR23213 HMMPANTHER 101 158 3
PTHR23213 HMMPANTHER 780 832 3
SSF101447 SuperFamily 881 1269 3
SSF101447 SuperFamily 806 817 3
SSF48371 SuperFamily IPR016024 Armadillo-type fold 112 496 130
Coil ncoils Predicted coiled-coil protein (DUF2205) 1245 1265 1055
Coil ncoils Predicted coiled-coil protein (DUF2205) 1153 1173 1055

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.49 Da
Charge 29.50
Codon Adaptation Index 0.39
Isoelectric point 8.56
Molecular weight 157.07 kDa
Number of residues 1372
Sequence
Gene Expression

Qualitative Gene Expression

DescriptionLevelEvidenceReference
RNA levelincreased during GO:0071444Northern assay evidencePMID:7791776
present during GO:0072690Northern assay evidencePMID:7791776

Quantitative Gene Expression

View graphical display of gene expression data for fus1 (SPAC20G4.02c)

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.42during GO:0072690PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.19during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000014,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
conserved in fungi4607
conserved in eukaryotes4517
conserved in metazoa3427
conserved in vertebrates3402
conserved in eukaryotes only2502
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC20G4.02c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
negative genetic interaction withtif38translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
negative genetic interaction withfhl1forkhead transcription factor Fhl1 Negative GeneticPMID:22681890
negative genetic interaction withclr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
negative genetic interaction withsrb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
negative genetic interaction withmcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
negative genetic interaction withrec14Ski complex subunit Rec14 Negative GeneticPMID:22681890
negative genetic interaction withssp2AMP-activated protein serine/threonine kinase alpha subunit Ssp2 Negative GeneticPMID:22681890
negative genetic interaction withdsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
negative genetic interaction withhip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
negative genetic interaction withsrp1SR family protein, human SRFS2 ortholog Srp1 Negative GeneticPMID:22681890
negative genetic interaction withnrm1MBF complex corepressor Nrm1 Negative GeneticPMID:22681890
negative genetic interaction withSPCC1450.09cphospholipase (predicted) Negative GeneticPMID:22681890
negative genetic interaction withusp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
negative genetic interaction withppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
positive genetic interaction withppk21serine/threonine protein kinase Ppk21 Positive GeneticPMID:22681890
positive genetic interaction withsap14U2 snRNP-associated protein SF3B14 Sap14 Positive GeneticPMID:22681890
positive genetic interaction withpil2meiotic eisosome BAR domain protein Pil2 Positive GeneticPMID:22681890
positive genetic interaction withiwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
positive genetic interaction withswd3WD repeat protein Swd3 Positive GeneticPMID:22681890
positive genetic interaction withsty1MAP kinase Sty1 Positive GeneticPMID:22681890
positive genetic interaction withsrb11cyclin CycC, Srb mediator subunit Srb11 Positive GeneticPMID:22681890
positive genetic interaction withdad1DASH complex subunit Dad1 Positive GeneticPMID:22681890
positive genetic interaction withshg1Set1C complex subunit Shg1 Positive GeneticPMID:22681890
positive genetic interaction withkin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
positive genetic interaction withjmj2histone demethylase Jmj2 Positive GeneticPMID:22681890
phenotype enhanced bycfr1Chs five related protein Cfr1 Phenotypic EnhancementPMID:16598689
phenotype enhanced byexg1glucan 1,6-beta-glucosidase Exg1 Phenotypic EnhancementPMID:25825517
phenotype enhanced byagn2glucan endo-1,3-alpha-glucosidase Agn2 Phenotypic EnhancementPMID:25825517
phenotype enhanced byagn1glucan endo-1,3-alpha-glucosidase Agn1 Phenotypic EnhancementPMID:25825517
phenotype enhanced byexg2glucan glucosidase Exg2, unknown specificity Phenotypic EnhancementPMID:25825517
phenotype enhanced byexg3glucan 1,3-beta-glucosidase Exg3 Phenotypic EnhancementPMID:25825517
phenotype enhanced byeng2endo-1,3-beta-glucanase Eng2 Phenotypic EnhancementPMID:25825517
phenotype enhanced byfor3formin For3 Phenotypic EnhancementPMID:25825517
enhances phenotype ofprm1conjugation protein Prm1 Phenotypic EnhancementPMID:24514900
External References
Database Identifier Description
NBRP SPAC20G4.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G4.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G4.02c BioGRID Interaction Datasets
Expression Viewer SPAC20G4.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G4.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G4.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G4.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G4.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G4.02c Transcriptome Viewer (Bähler Lab)
GEO SPAC20G4.02c GEO profiles
PInt SPAC20G4.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC20G4.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC20G4.02c Fission yeast phenotypic data & analysis
Cyclebase SPAC20G4.02c.1 Cell Cycle Data
SPD / RIKEN30/30F08Orfeome Localization Data
UniProtKB/SwissProtQ10719Cell fusion protein fus1
ModBaseQ10719Database of comparative protein structure models
STRINGQ10719Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594737formin Fus1
RefSeq mRNANM_001020165972h- formin Fus1 (fus1), mRNA
European Nucleotide ArchiveAAA99003.1ENA Protein Mapping
European Nucleotide ArchiveCAB11252.1ENA Protein Mapping
UniParcUPI000012AD8EUniProt Archive

Literature for fus1

Search: Europe PMC or PubMed

Release Version: PomBase:30_61 - 12 Sep 2016