fus1 (SPAC20G4.02c)

Gene Standard Namefus1 Characterisation Statuspublished
Systematic IDSPAC20G4.02c Feature Typeprotein coding
Synonyms Name Description
Productformin Fus1 Product Size1372aa, 157.07 kDa
Genomic Location Chromosome I, 4817790-4813111 (4680nt); CDS:4817343-4813225 (4119nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
actin binding38
Annotation ExtensionEvidenceWith/FromReference
Rho GTPase binding4
Annotation ExtensionEvidenceWith/FromReference
SH3 domain binding1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
actin cytoskeleton organization102
Annotation ExtensionEvidenceWith/FromReference
actin filament bundle assembly12
Annotation ExtensionEvidenceWith/FromReference
actin nucleation16
Annotation ExtensionEvidenceWith/FromReference
plasma membrane fusion involved in cytogamy6
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
cell division site301
Annotation ExtensionEvidenceWith/FromReference
cell-cell junction2
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
Annotation ExtensionEvidenceWith/FromReference
mating projection8
Annotation ExtensionEvidenceWith/FromReference
mating projection tip7
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationCell growth assayfus1ΔNullPMID:77917763759
Microscopyfus1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001370abnormal protein localization262
affecting dni1Microscopyfus1ΔNullPMID:19627505
FYPO:0003363abolished cytogamyMicroscopyfus1-B20Not specifiedPMID:77917762
FYPO:0002044abolished premeiotic DNA replicationOtherfus1-B20Not specifiedPMID:447273314
FYPO:0000708decreased mating efficiencyCell growth assayTR1 and TR2 deletion (deletion of both TR boxes in promoter)Not specifiedPMID:7791776240
FYPO:0003364mislocalized nucleus during matingMicroscopyfus1-B20Not specifiedPMID:77917761
FYPO:0003135normal agglutinationMicroscopyfus1-B20Not specifiedPMID:77917762
FYPO:0001147normal mating efficiencyCell growth assayTR1 deleted (TR1 promoter box at -157 deleted)Not specifiedPMID:779177620
Cell growth assayTR2 deleted (TR2 promoter box at -238 deleted)Not specifiedPMID:7791776
FYPO:0000573normal shmoo formationMicroscopyfus1-B20Not specifiedPMID:77917763
FYPO:0000590normal sporulationMicroscopyfus1-B20/fus1-B20 (homozygous diploid)Not specifiedPMID:779177645
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopyfus1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Target Of
FYPO affected by mutation in byr1 MAP kinase kinase Byr1 PMID:7791776
FYPO affected by mutation in byr2 MAP kinase kinase kinase Byr2 PMID:7791776
FYPO affected by mutation in ste11 transcription factor Ste11 PMID:7791776
GO regulated by ste11 transcription factor Ste11 PMID:17032641
Ensembl transcript structure with UTRs, exons and introns


Exon Start End


Region Coordinates Reference
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF06371 Pfam IPR010473 Formin, GTPase-binding domain 106 296 4
PF02181 Pfam IPR015425 Formin, FH2 domain 870 1253 3
SM00498 SMART IPR015425 Formin, FH2 domain 868 1328 3
PS51444 Prosite Profiles IPR015425 Formin, FH2 domain 868 1278 3
PS51232 Prosite Profiles IPR014768 Formin, GTPase-binding and FH3 domain 104 522 3
PTHR23213:SF6 HMMPANTHER 134 1312 1
PTHR23213 HMMPANTHER 134 1312 3
SSF101447 SuperFamily 881 1269 3
SSF101447 SuperFamily 806 817 3
SSF48371 SuperFamily IPR016024 Armadillo-type fold 112 496 130
Coil ncoils Rabaptin coiled-coil domain 1153 1174 968
Coil ncoils Rabaptin coiled-coil domain 1245 1266 968

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.49 Da
Charge 29.50
Isoelectric point 8.56
Molecular weight 157.07 kDa
Number of residues 1372
Gene Expression

Qualitative Gene Expression

RNA levelincreased during GO:0071444Northern assay evidencePMID:7791776
present during GO:0072690Northern assay evidencePMID:7791776

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
0.42during GO:0072690PECO:0000005,
population_wideexperimental evidencePMID:23101633
0.19during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
population_wideexperimental evidencePMID:23101633
Species Distribution
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
dad1DASH complex subunit Dad1 Positive GeneticPMID:22681890
shg1Set1C complex subunit Shg1 Positive GeneticPMID:22681890
iwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
swd3WD repeat protein Swd3 Positive GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
SPCC1450.09cphospholipase (predicted) Negative GeneticPMID:22681890
srp1SR family protein, human SRFS2 ortholog Srp1 Negative GeneticPMID:22681890
jmj2histone demethylase Jmj2 Positive GeneticPMID:22681890
rec14Ski complex subunit Rec14 Negative GeneticPMID:22681890
sap14U2 snRNP-associated protein SF3B14 Sap14 Positive GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Positive GeneticPMID:22681890
nrm1MBF complex corepressor Nrm1 Negative GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
kin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
ppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
cfr1Chs five related protein Cfr1 Phenotypic EnhancementPMID:16598689
ppk21serine/threonine protein kinase Ppk21 Positive GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
usp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
ssp2serine/threonine protein kinase Ssp2 Negative GeneticPMID:22681890
mcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
srb11cyclin CycC, Srb mediator subunit Srb11 Positive GeneticPMID:22681890
fhl1forkhead transcription factor Fhl1 Negative GeneticPMID:22681890
pil2meiotic eisosome BAR domain protein Pil2 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC20G4.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G4.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G4.02c BioGRID Interaction Datasets
Expression Viewer SPAC20G4.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G4.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G4.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G4.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G4.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G4.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC20G4.02c Cell Cycle Data
GEO SPAC20G4.02c GEO profiles
PInt SPAC20G4.02c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC20G4.02c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC20G4.02c Fission yeast phenotypic data & analysis
SPD / RIKEN30/30F08Orfeome Localization Data
UniProtKB/SwissProtQ10719Cell fusion protein fus1
ModBaseQ10719Database of comparative protein structure models
STRINGQ10719Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594737formin Fus1
RefSeq mRNANM_001020165972h- formin Fus1 (fus1), mRNA
European Nucleotide ArchiveAAA99003.1ENA Protein Mapping
European Nucleotide ArchiveCAB11252.1ENA Protein Mapping
UniParcUPI000012AD8EUniProt Archive

Literature for fus1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014