fus1 (SPAC20G4.02c)


Gene Standard Namefus1 Characterisation Statuspublished
Systematic IDSPAC20G4.02c Feature Typeprotein coding
Synonyms Name Description
Productformin Fus1 Product Size1372aa, 157.07 kDa
Genomic Location Chromosome I, 4817790-4813111 (4680nt); CDS:4817343-4813225 (4119nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0017048Rho GTPase bindingIEAIPR010473GO_REF:00000024
GO:0017124SH3 domain bindingIEAUniProtKB-KW:KW-0729GO_REF:00000371
GO:0003779actin bindingIEAIPR010473GO_REF:000000238
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030036actin cytoskeleton organizationISSUniProtKB:P41832GO_REF:000000197
GO:0051017actin filament bundle assemblyNASGO_REF:00000019
GO:0032220plasma membrane fusion involved in cytogamy6
requiredIMPPMID:7791776
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0032153cell division siteIDAPMID:16823372292
GO:0005737cytoplasmIDAPMID:96062134198
IDAPMID:16823372
GO:0005829cytosolIDAPMID:168233722317
GO:0005937mating projection7
during conjugation with cellular fusionIDAPMID:9606213
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0002060viable vegetative cell populationMicroscopyfus1ΔNullPECO:0000005, PECO:0000137PMID:236978063755
Microscopyfus1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0001370abnormal protein localizationMicroscopyfus1ΔNullPMID:19627505142
affecting dni1
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopyfus1ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Target Of
RelationshipGeneProductReference
regulated by ste11 transcription factor Ste11 PMID:17032641
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
148177904813111

UTRs

Region Start End Reference
three_prime_UTR48132244813111PMID:21511999
five_prime_UTR48177904817344PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF06371 Pfam IPR010473 Formin, GTPase-binding domain 106 296 4
PF02181 Pfam IPR015425 Formin, FH2 domain 870 1253 3
SM00498 SMART IPR015425 Formin, FH2 domain 868 1328 3
PS51232 Prosite Profiles IPR014768 Formin, GTPase-binding and FH3 domain 104 522 3
PS51444 Prosite Profiles IPR015425 Formin, FH2 domain 868 1278 3
PTHR23213:SF6 HMMPANTHER 134 1312 1
PTHR23213 HMMPANTHER 134 1312 3
SSF48371 SuperFamily IPR016024 112 496 130
SSF101447 SuperFamily 881 1269 3
SSF101447 SuperFamily 806 817 3
Coil ncoils Rabaptin coiled-coil domain 1245 1266 975
Coil ncoils Rabaptin coiled-coil domain 1153 1174 975
Low complexity (SEG) seg 334 349
Low complexity (SEG) seg 698 709
Low complexity (SEG) seg 805 819
Low complexity (SEG) seg 840 851
Low complexity (SEG) seg 1217 1226

View domain organization at Pfam

Protein Properties

Ave. residue weight 114.49 Da
Charge 29.50
Isoelectric point 8.56
Molecular weight 157.07 kDa
Number of residues 1372
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
NDduring cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
NDduring GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.19during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
0.42during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
dad1DASH complex subunit Dad1 Positive GeneticPMID:22681890
shg1Set1C complex subunit Shg1 Positive GeneticPMID:22681890
iwr1RNA polymerase II nuclear import protein Iwr1 (predicted) Positive GeneticPMID:22681890
swd3WD repeat protein Swd3 Positive GeneticPMID:22681890
clr4histone H3 lysine methyltransferase Clr4 Negative GeneticPMID:22681890
SPCC1450.09cphospholipase (predicted) Negative GeneticPMID:22681890
srp1SR family protein, human SRFS2 ortholog Srp1 Negative GeneticPMID:22681890
jmj2histone demethylase Jmj2 Positive GeneticPMID:22681890
rec14Ski complex subunit Rec14 Negative GeneticPMID:22681890
sap14U2 snRNP-associated protein SF3B14 Sap14 Positive GeneticPMID:22681890
SPAC821.05translation initiation factor eIF3h (p40) Negative GeneticPMID:22681890
sty1MAP kinase Sty1 Positive GeneticPMID:22681890
ppk21serine/threonine protein kinase Ppk21 Positive GeneticPMID:22681890
dsc3Golgi Dsc E3 ligase complex subunit Dsc3 Negative GeneticPMID:21504829
kin1microtubule affinity-regulating kinase Kin1 Positive GeneticPMID:22681890
ppk14serine/threonine protein kinase Ppk14 (predicted) Negative GeneticPMID:22681890
srb10cyclin-dependent protein Srb mediator subunit kinase Srb10 Negative GeneticPMID:22681890
cfr1Chs five related protein Cfr1 Phenotypic EnhancementPMID:16598689
nrm1MBF complex corepressor nrm1 Negative GeneticPMID:22681890
hip4histone promoter control protein Hip4 Negative GeneticPMID:22681890
usp102U1 snRNP-associated protein Usp102 Negative GeneticPMID:22681890
ssp2serine/threonine protein kinase Ssp2 Negative GeneticPMID:22681890
mcs2TFIIH complex cyclin Mcs2 Negative GeneticPMID:22681890
srb11cyclin CycC, Srb mediator subunit Srb11 Positive GeneticPMID:22681890
fhl1forkhead transcription factor Fhl1 Negative GeneticPMID:22681890
pil2meiotic eisosome BAR domain protein Pil2 Positive GeneticPMID:22681890
External References
Database Identifier Description
NBRP SPAC20G4.02c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G4.02c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G4.02c BioGRID Interaction Datasets
Expression Viewer SPAC20G4.02c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G4.02c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G4.02c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G4.02c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G4.02c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G4.02c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC20G4.02c Cell Cycle Data
GEO SPAC20G4.02c GEO profiles
PInt SPAC20G4.02c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2541741formin Fus1
EntrezGene2541741formin Fus1
SPD / RIKEN30/30F08Orfeome Localization Data
UniProtKB/SwissProtQ10719Cell fusion protein fus1
ModBaseQ10719Database of comparative protein structure models
StringQ10719Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594737formin Fus1
RefSeq mRNANM_001020165972h- formin Fus1 (fus1), mRNA
European Nucleotide ArchiveAAA99003ENA Protein Mapping
European Nucleotide ArchiveCAB11252ENA Protein Mapping
UniParcUPI000012AD8EUniProt Archive

Literature for fus1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014