sts1 (SPAC20G4.07c)

Gene Standard Namests1 Characterisation Statuspublished
Systematic IDSPAC20G4.07c Feature Typeprotein coding
Synonymserg4 Name Descriptionstaurosporine supersensitive
ProductC-24(28) sterol reductase Sts1 Product Size453aa, 52.55 kDa
Genomic Location Chromosome I, 4828141-4826601 (1541nt); CDS:4828044-4826683 (1362nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
delta24(24-1) sterol reductase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ascospore formation62
Annotation ExtensionEvidenceWith/FromReference
cellular response to cation stress8
Annotation ExtensionEvidenceWith/FromReference
cellular response to osmotic stress35
Annotation ExtensionEvidenceWith/FromReference
ergosterol biosynthetic process45
Annotation ExtensionEvidenceWith/FromReference
establishment of protein localization to plasma membrane18
Annotation ExtensionEvidenceWith/FromReference
protein insertion into membrane raft4
Annotation ExtensionEvidenceWith/FromReference
NOT endocytosis
Annotation ExtensionEvidenceWith/FromReference
NOT protein targeting to vacuole
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum membrane203
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane967
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Single-allele Phenotypes

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at low temperaturests1Δ68
decreased cell population growth on glucose carbon sourcests1Δ268
normal cell population growth at high temperaturests1Δ52
normal growth on glucose carbon sourcests1Δ225
normal growth on hydroxyureasts1Δ43
normal growth on pravastatinsts1Δ2
normal growth on thiabendazolests1Δ20
resistance to amphotericin Bsts1Δ9
resistance to nystatinsts1Δ6
sensitive to caffeine during vegetative growthsts1Δ106
sensitive to chloramphenicolsts1Δ1
sensitive to clotrimazolests1Δ105
sensitive to cycloheximidests1Δ30
sensitive to cyclosporin Asts1Δ7
sensitive to micafunginsts1Δ116
sensitive to rapamycinsts1Δ78
sensitive to sodium dodecyl sulfatests1Δ6
sensitive to sorbitolsts1Δ11
sensitive to staurosporinests1Δ10
sensitive to terbinafinests1Δ102
viable vegetative cell populationsts1Δ3815

Cell Phenotype

Term NameGenotypesCount
abnormal microtubule cytoskeleton morphology during mitotic interphasests1Δ186
abnormal sporulation233
elongated multinucleate multiseptate vegetative cell, septa between nucleists1Δ17
ergosterol absent from cellsts1Δ1
viable cell at high temperaturests1Δ8
viable vegetative cellsts1Δ3639
viable vegetative cell with normal cell morphologysts1Δ3095
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
5' UTR4828141..4828045AU010943
3' UTR4826682..4826601PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01222 Pfam IPR001171 Ergosterol biosynthesis ERG4/ERG24 8 453 2
TMhelix TMHMM 210 229 959
TMhelix TMHMM 150 172 959
TMhelix TMHMM 118 140 959
TMhelix TMHMM 13 35 959
TMhelix TMHMM 75 97 959
TMhelix TMHMM 277 299 959
TMhelix TMHMM 304 326 959
TMhelix TMHMM 396 418 959
PS01017 Prosite Patterns IPR018083 Sterol reductase, conserved site 185 200 2
PS01018 Prosite Patterns IPR018083 Sterol reductase, conserved site 417 440 2
PTHR21257:SF31 HMMPANTHER 2 453 1
PTHR21257 HMMPANTHER 2 453 2

View domain organization at Pfam

Term IDTerm NameReferenceCount

Protein Properties

Ave. residue weight 116.00 Da
Charge 23.00
Codon Adpatation Index 0.50
Isoelectric point 9.05
Molecular weight 52.55 kDa
Number of residues 453
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4498during GO:0000080PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
6600during GO:0000084PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
4090during GO:0000085PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
5625during GO:0000087PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
5338.44during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
5697during GO:0072690PECO:0000005,
single cellmass spectrometry evidencePMID:24763107
1870.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.2during GO:0072690PECO:0000014,
population wideexperimental evidencePMID:23101633
0.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
predominantly single copy (one to one)3090
conserved in fungi4604
conserved in eukaryotes4514
conserved in metazoa3421
conserved in vertebrates3396
conserved in eukaryotes only2503

Manually curated orthologous groups


Orthologs in Compara

Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic LethalityPMID:7983142
External References
Database Identifier Description
NBRP SPAC20G4.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G4.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G4.07c BioGRID Interaction Datasets
Expression Viewer SPAC20G4.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G4.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G4.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G4.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G4.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G4.07c Transcriptome Viewer (Bähler Lab)
GEO SPAC20G4.07c GEO profiles
PInt SPAC20G4.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC20G4.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC20G4.07c Fission yeast phenotypic data & analysis
Cyclebase SPAC20G4.07c.1 Cell Cycle Data
IntEnz1.3.1.71Integrated relational Enzyme database
Rhea1.3.1.71Annotated reactions database
SPD / RIKEN44/44C10Orfeome Localization Data
UniProtKB/SwissProtP36209Delta(24(24(1)))-sterol reductase
ModBaseP36209Database of comparative protein structure models
STRINGP36209Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594742C-24(28) sterol reductase Sts1
RefSeq mRNANM_001020170972h- C-24(28) sterol reductase Sts1 (sts1), mRNA
European Nucleotide ArchiveCU329670ENA EMBL mapping
European Nucleotide ArchiveX63549ENA EMBL mapping
European Nucleotide ArchiveCAA45113ENA Protein Mapping
European Nucleotide ArchiveCAA45113.1ENA Protein Mapping
European Nucleotide ArchiveCAB11256ENA Protein Mapping
European Nucleotide ArchiveCAB11256.1ENA Protein Mapping
UniParcUPI000013614FUniProt Archive

Literature for sts1

Search: Europe PMC or PubMed

Release Version: PomBase:28_55 - 09 Sep 2015