sts1 (SPAC20G4.07c)


Gene Standard Namests1 Characterisation Statuspublished
Systematic IDSPAC20G4.07c Feature Typeprotein coding
Synonymserg4 Name Descriptionstaurosporine supersensitive
ProductC-24(28) sterol reductase Sts1 Product Size453aa, 52.55 kDa
Genomic Location Chromosome I, 4828141-4826601 (1541nt); CDS:4828044-4826683 (1362nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
delta24(24-1) sterol reductase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ascospore formation75
Annotation ExtensionEvidenceWith/FromReference
cellular response to cation stress8
Annotation ExtensionEvidenceWith/FromReference
cellular response to osmotic stress35
Annotation ExtensionEvidenceWith/FromReference
ergosterol biosynthetic process44
Annotation ExtensionEvidenceWith/FromReference
establishment of protein localization to plasma membrane19
Annotation ExtensionEvidenceWith/FromReference
protein insertion into membrane raft4
Annotation ExtensionEvidenceWith/FromReference
NOT endocytosis
Annotation ExtensionEvidenceWith/FromReference
NOT protein targeting to vacuole
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum membrane205
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000674normal cell population growth at high temperatureCell growth assaysts1ΔNullPECO:0000201, PECO:0000102PMID:2314504837
FYPO:0001164normal growth on glucose carbon sourceCell growth assaysts1ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:23145048176
FYPO:0000963normal growth on hydroxyureaCell growth assaysts1ΔNullPMID:132096022
FYPO:0002329normal growth on pravastatinCell growth assaysts1ΔNullPECO:0000201, PECO:0000005PMID:231450482
FYPO:0000964normal growth on thiabendazoleCell growth assaysts1ΔNullPMID:13209609
FYPO:0000070resistance to amphotericin BCell growth assaysts1ΔNullPECO:0000137, PECO:0000005PMID:222528179
Cell growth assaysts1ΔNullPMID:18310029
FYPO:0000076resistance to nystatinCell growth assaysts1ΔNullPMID:183100296
FYPO:0000097sensitive to caffeineCell growth assaysts1ΔNullPMID:1320960105
FYPO:0000100sensitive to chloramphenicolCell growth assaysts1ΔNullPMID:13209601
FYPO:0002640sensitive to clotrimazole105
expressivity FYPO_EXT:0000001Cell growth assaysts1ΔNullPECO:0000137, PECO:0000005PMID:22252817
FYPO:0000080sensitive to coldCell growth assaysts1ΔNullPMID:132096043
FYPO:0000104sensitive to cycloheximideCell growth assaysts1ΔNullPMID:1831002918
FYPO:0002641sensitive to micafunginCell growth assaysts1ΔNullPECO:0000137, PECO:0000005PMID:22252817116
FYPO:0000841sensitive to sodium dodecyl sulfateCell growth assaysts1ΔNullPMID:13209605
FYPO:0000112sensitive to sorbitolCell growth assaysts1ΔNullPMID:13209607
FYPO:0000113sensitive to staurosporineCell growth assaysts1EndogenousPMID:13209608
Cell growth assaysts1ΔNullPMID:1320960
Cell growth assaysts1-5Not specifiedPECO:0000201, PECO:0000102, PECO:0000005PMID:1899230
FYPO:0002328sensitive to terbinafine101
expressivity FYPO_EXT:0000001Cell growth assaysts1ΔNullPECO:0000137, PECO:0000005PMID:22252817
FYPO:0002060viable vegetative cell populationMicroscopysts1ΔNullPMID:204732893759
Microscopysts1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0003625abnormal microtubule cytoskeleton morphology during mitotic interphase186
penetrance FYPO_EXT:0000002experimental phenotypic evidencests1ΔNullPECO:0000005, PECO:0000103, PECO:0000137DOI:10.1016/j.devcel.2014.09.005
FYPO:0000121abnormal sporulationMicroscopynot recorded (unrecorded)PMID:18310029193
FYPO:0002313ergosterol absent from cellSubstance quantification evidencests1ΔNullPMID:231450481
FYPO:0000282viable cell at high temperatureCell growth assaysts1ΔNullPECO:0000201, PECO:0000102PMID:231450488
FYPO:0001491viable vegetative cellCell growth assaysts1ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:231450483607
Cell growth assaysts1ΔNullPMID:1320960
FYPO:0002177viable vegetative cell with normal cell morphology3089
penetrance FYPO_EXT:0000001Microscopysts1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
148281414826601

UTRs

Region Coordinates Reference
three_prime_UTR4826682..4826601PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01222 Pfam IPR001171 Ergosterol biosynthesis ERG4/ERG24 8 453 2
TMhelix TMHMM 396 418 959
TMhelix TMHMM 210 229 959
TMhelix TMHMM 277 299 959
TMhelix TMHMM 118 140 959
TMhelix TMHMM 13 35 959
TMhelix TMHMM 75 97 959
TMhelix TMHMM 304 326 959
TMhelix TMHMM 150 172 959
PS01018 Prosite Patterns IPR018083 Sterol reductase, conserved site 417 440 2
PS01017 Prosite Patterns IPR018083 Sterol reductase, conserved site 185 200 2
PTHR21257 HMMPANTHER 2 453 2
PTHR21257:SF4 HMMPANTHER 2 453 1

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 116.00 Da
Charge 23.00
Isoelectric point 9.05
Molecular weight 52.55 kDa
Number of residues 453
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4498during GO:0000080PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
6600during GO:0000084PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
4090during GO:0000085PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
5625during GO:0000087PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
5697during GO:0072690PECO:0000126,
PECO:0000005
single_cellmass spectrometry evidencePMID:24763107
5338.44during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
1870.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
0.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4600
conserved in eukaryotes4514
conserved in metazoa3425
conserved in vertebrates3400
conserved in eukaryotes only2497
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
ppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic LethalityPMID:7983142
External References
Database Identifier Description
NBRP SPAC20G4.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G4.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G4.07c BioGRID Interaction Datasets
Expression Viewer SPAC20G4.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G4.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G4.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G4.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G4.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G4.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC20G4.07c Cell Cycle Data
GEO SPAC20G4.07c GEO profiles
PInt SPAC20G4.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC20G4.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC20G4.07c Fission yeast phenotypic data & analysis
SPD / RIKEN44/44C10Orfeome Localization Data
IntEnz1.3.1.71Integrated relational Enzyme database
Rhea1.3.1.71Annotated reactions database
UniProtKB/SwissProtP36209Delta(24(24(1)))-sterol reductase
ModBaseP36209Database of comparative protein structure models
STRINGP36209Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594742C-24(28) sterol reductase Sts1
RefSeq mRNANM_001020170972h- C-24(28) sterol reductase Sts1 (sts1), mRNA
European Nucleotide ArchiveCAA45113.1ENA Protein Mapping
European Nucleotide ArchiveCAB11256.1ENA Protein Mapping
UniParcUPI000013614FUniProt Archive

Literature for sts1

Search: Europe PMC or PubMed

Release Version: PomBase:23_47 - 27 Oct 2014