sts1 (SPAC20G4.07c)


Gene Standard Namests1 Characterisation Statuspublished
Systematic IDSPAC20G4.07c Feature Typeprotein coding
Synonymserg4 Name Descriptionstaurosporine supersensitive
ProductC-24(28) sterol reductase Sts1 Product Size453aa, 52.55 kDa
Genomic Location Chromosome I, 4828141-4826601 (1541nt); CDS:4828044-4826683 (1362nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
delta24(24-1) sterol reductase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ascospore formation63
Annotation ExtensionEvidenceWith/FromReference
cellular response to cation stress8
Annotation ExtensionEvidenceWith/FromReference
cellular response to osmotic stress34
Annotation ExtensionEvidenceWith/FromReference
ergosterol biosynthetic process44
Annotation ExtensionEvidenceWith/FromReference
establishment of protein localization to plasma membrane20
Annotation ExtensionEvidenceWith/FromReference
protein insertion into membrane raft4
Annotation ExtensionEvidenceWith/FromReference
NOT endocytosis
Annotation ExtensionEvidenceWith/FromReference
NOT protein targeting to vacuole
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum membrane206
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
decreased cell population growth at low temperaturests1ΔNull61
decreased cell population growth on glucose carbon sourcests1ΔNull258
normal cell population growth at high temperaturests1ΔNull47
normal growth on glucose carbon sourcests1ΔNull207
normal growth on hydroxyureasts1ΔNull42
normal growth on pravastatinsts1ΔNull2
normal growth on thiabendazolests1ΔNull13
resistance to amphotericin Bsts1ΔNull9
resistance to nystatinsts1ΔNull6
sensitive to caffeine during vegetative growthsts1ΔNull106
sensitive to chloramphenicolsts1ΔNull1
sensitive to clotrimazolests1ΔNull105
sensitive to cycloheximidests1ΔNull30
sensitive to cyclosporin Asts1ΔNull7
sensitive to micafunginsts1ΔNull116
sensitive to rapamycinsts1ΔNull78
sensitive to sodium dodecyl sulfatests1ΔNull6
sensitive to sorbitolsts1ΔNull11
sensitive to staurosporinests1Endogenous9
sts1-5Not specified
sts1ΔNull
sensitive to terbinafinests1ΔNull102
viable vegetative cell populationsts1ΔNull3809

Cell Phenotype

Term NameAlleleExpressionCount
abnormal microtubule cytoskeleton morphology during mitotic interphasests1ΔNull186
abnormal sporulationunnamed (unrecorded)208
elongated multinucleate multiseptate vegetative cell, septa between nucleists1ΔNull17
ergosterol absent from cellsts1ΔNull1
viable cell at high temperaturests1ΔNull8
viable vegetative cellsts1ΔNull3524
viable vegetative cell with normal cell morphologysts1ΔNull3094
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4828141..4826601
mRNA4828141..4826601
5' UTR4828141..4828045AU010943
CDS4828044..4826683
3' UTR4826682..4826601PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01222 Pfam IPR001171 Ergosterol biosynthesis ERG4/ERG24 8 453 2
TMhelix TMHMM 277 299 959
TMhelix TMHMM 210 229 959
TMhelix TMHMM 75 97 959
TMhelix TMHMM 13 35 959
TMhelix TMHMM 396 418 959
TMhelix TMHMM 150 172 959
TMhelix TMHMM 304 326 959
TMhelix TMHMM 118 140 959
PS01018 Prosite Patterns IPR018083 Sterol reductase, conserved site 417 440 2
PS01017 Prosite Patterns IPR018083 Sterol reductase, conserved site 185 200 2
PTHR21257:SF31 HMMPANTHER 2 453 1
PTHR21257 HMMPANTHER 2 453 2

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 116.00 Da
Charge 23.00
Isoelectric point 9.05
Molecular weight 52.55 kDa
Number of residues 453
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4498during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6600during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4090during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5625during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5338.44during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
5697during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
1870.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4603
conserved in eukaryotes4514
conserved in metazoa3424
conserved in vertebrates3399
conserved in eukaryotes only2501
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic LethalityPMID:7983142
External References
Database Identifier Description
NBRP SPAC20G4.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G4.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G4.07c BioGRID Interaction Datasets
Expression Viewer SPAC20G4.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G4.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G4.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G4.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G4.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G4.07c Transcriptome Viewer (Bähler Lab)
GEO SPAC20G4.07c GEO profiles
PInt SPAC20G4.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC20G4.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC20G4.07c Fission yeast phenotypic data & analysis
Cyclebase SPAC20G4.07c.1 Cell Cycle Data
SPD / RIKEN44/44C10Orfeome Localization Data
IntEnz1.3.1.71Integrated relational Enzyme database
Rhea1.3.1.71Annotated reactions database
UniProtKB/SwissProtP36209Delta(24(24(1)))-sterol reductase
ModBaseP36209Database of comparative protein structure models
STRINGP36209Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594742C-24(28) sterol reductase Sts1
RefSeq mRNANM_001020170972h- C-24(28) sterol reductase Sts1 (sts1), mRNA
European Nucleotide ArchiveCAA45113.1ENA Protein Mapping
European Nucleotide ArchiveCAB11256.1ENA Protein Mapping
UniParcUPI000013614FUniProt Archive

Literature for sts1

Search: Europe PMC or PubMed

Release Version: PomBase:26_53 - 27 May 2015