erg4 (SPAC20G4.07c)


Gene Standard Nameerg4 Characterisation Statuspublished
Systematic IDSPAC20G4.07c Feature Typeprotein coding
Synonymssts1 Name Descriptionstaurosporine supersensitive
ProductC-24(28) sterol reductase Erg4 Product Size453aa, 52.55 kDa
Genomic Location Chromosome I, 4828141-4826601 (1541nt); CDS:4828044-4826683 (1362nt)

Ensembl Gene Location
GO Molecular Function
GO Biological Process
Term NameCount
ergosterol biosynthetic process44
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum membrane203
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane969
Annotation ExtensionEvidenceWith/FromReference
FYPO Single-Allele Phenotypes
Gene Deletion Viability: Viable

Population Phenotype

Term NameGenotypesCount
decreased cell population growth at low temperaturests1Δ96
decreased cell population growth on glucose carbon sourceerg4Δ393
decreased mating efficiencyerg4Δ276
normal cell population growth at high temperaturests1Δ77
normal growth on glucose carbon sourcests1Δ318
normal growth on hydroxyureasts1Δ76
normal growth on pravastatinsts1Δ2
normal growth on thiabendazolests1Δ26
resistance to amphotericin Berg4Δ8
sensitive to arsenicerg4Δ118
sensitive to cadmiumerg4Δ321
sensitive to caffeine during vegetative growthsts1Δ110
sensitive to chloramphenicolsts1Δ1
sensitive to clotrimazoleerg4Δ108
sensitive to cycloheximideerg4Δ31
sensitive to cyclosporin Asts1Δ7
sensitive to micafunginerg4Δ119
sensitive to rapamycinerg4Δ80
sensitive to sodium dodecyl sulfatests1Δ10
sensitive to sorbitolsts1Δ13
sensitive to staurosporinests115
erg4Δ
sts1-5
sts1Δ
sensitive to terbinafineerg4Δ103
viable vegetative cell populationerg4Δ3862
sts1Δ

Cell Phenotype

Term NameGenotypesCount
abnormal microtubule cytoskeleton morphology during mitotic interphaseerg4Δ187
abolished protein localization to plasma membrane, with protein mislocalized to cytoplasm, during vegetative growth12
affects localization of aat1erg4Δ
elongated multiseptate vegetative cell, with three or more nuclei and single septa between nucleists1Δ17
ergosterol absent from cellerg4Δ3
sts1Δ
increased cellular ergosta-5,7,22,24(28)-tetraen-3beta-ol levelerg4Δ1
normal endocytosiserg4Δ10
normal protein localization to plasma membrane15
affecting bfr1erg4Δ
affecting pmd1erg4Δ
normal vacuole fusion during cellular hypotonic responseerg4Δ8
small vacuoles during vegetative growtherg4Δ9
viable cell at high temperaturests1Δ6
viable vegetative cellsts1Δ3680
viable vegetative cell with normal cell morphologysts1Δ3103

FYPO Multi-allele Phenotypes

Population Phenotype

Term NameGenotypes
decreased vegetative cell population growthppb1::ura4+, erg4Δ
inviable vegetative cell populationppb1::ura4+, erg4Δ

Cell Phenotype

Term NameGenotypes
branched, elongated cell with branch forming adjacent to septumppb1::ura4+, erg4Δ
elongated multinucleate multiseptate vegetative cell, single septa between nucleierg4Δ, ppb1::ura4+
Transcript
Ensembl transcript structure with UTRs, exons and introns

Transcript Structure

Region Coordinates Reference
Exons4828141..4826601
mRNA4828141..4826601
5' UTR4828141..4828045AU010943
CDS4828044..4826683
3' UTR4826682..4826601PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01222 Pfam IPR001171 Ergosterol biosynthesis ERG4/ERG24 8 453 2
TMhelix TMHMM 13 35 950
TMhelix TMHMM 150 172 950
TMhelix TMHMM 118 140 950
TMhelix TMHMM 75 97 950
TMhelix TMHMM 304 326 950
TMhelix TMHMM 277 299 950
TMhelix TMHMM 210 229 950
TMhelix TMHMM 396 418 950
PS01017 Prosite Patterns IPR018083 Sterol reductase, conserved site 185 200 2
PS01018 Prosite Patterns IPR018083 Sterol reductase, conserved site 417 440 2
PTHR21257:SF31 HMMPANTHER 1 453 1
PTHR21257 HMMPANTHER 1 453 2

View domain organization at Pfam

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 116.00 Da
Charge 23.00
Codon Adaptation Index 0.50
Isoelectric point 9.05
Molecular weight 52.55 kDa
Number of residues 453
Sequence
Gene Expression

Quantitative Gene Expression

View graphical display of gene expression data for erg4 (SPAC20G4.07c)

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4498during GO:0000080PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
6600during GO:0000084PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
4090during GO:0000085PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5625during GO:0000087PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
5338.44during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
5697during GO:0072690PECO:0000005,
PECO:0000126
single cellmass spectrometry evidencePMID:24763107
1870.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.2during GO:0072690PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
0.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000127,
PECO:0000126,
PECO:0000005
population wideexperimental evidencePMID:23101633
Taxonomic Conservation
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3087
conserved in fungi4608
conserved in eukaryotes4516
conserved in metazoa3498
conserved in vertebrates3473
conserved in eukaryotes only2525
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Load genes that interact genetically with SPAC20G4.07c into the Query Builder
View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic LethalityPMID:7983142
External References
Database Identifier Description
NBRP SPAC20G4.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G4.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G4.07c BioGRID Interaction Datasets
Expression Viewer SPAC20G4.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G4.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G4.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G4.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G4.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G4.07c Transcriptome Viewer (Bähler Lab)
GEO SPAC20G4.07c GEO profiles
PInt SPAC20G4.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC20G4.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC20G4.07c Fission yeast phenotypic data & analysis
Cyclebase SPAC20G4.07c.1 Cell Cycle Data
IntEnz1.3.1.71Integrated relational Enzyme database
Rhea1.3.1.71Annotated reactions database
SPD / RIKEN44/44C10Orfeome Localization Data
UniProtKB/SwissProtP36209Delta(24(24(1)))-sterol reductase
ModBaseP36209Database of comparative protein structure models
STRINGP36209Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594742C-24(28) sterol reductase Sts1
RefSeq mRNANM_001020170972h- C-24(28) sterol reductase Sts1 (sts1), mRNA
European Nucleotide ArchiveCAA45113.1ENA Protein Mapping
European Nucleotide ArchiveCAB11256.1ENA Protein Mapping
UniParcUPI000013614FUniProt Archive

Literature for erg4

Search: Europe PMC or PubMed

Release Version: PomBase:30_62 - 30 Jan 2017