sts1 (SPAC20G4.07c)


Gene Standard Namests1 Characterisation Statuspublished
Systematic IDSPAC20G4.07c Feature Typeprotein coding
Synonymserg4 Name Descriptionstaurosporine supersensitive
ProductC-24(28) sterol reductase Sts1 Product Size453aa, 52.55 kDa
Genomic Location Chromosome I, 4828141-4826601 (1541nt); CDS:4828044-4826683 (1362nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000246delta24(24-1) sterol reductase activityISSUniProtKB:P25340GO_REF:00000011
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030437ascospore formationIMPPMID:1831002967
GO:0071473cellular response to cation stressIMPPMID:13209609
GO:0071470cellular response to osmotic stressIMPPMID:132096043
GO:0006696ergosterol biosynthetic processIMPPMID:1831002939
IMPPMID:23145048
GO:0090002establishment of protein localization to plasma membrane18
localizes aat1IMPPMID:18310029
GO:0071210protein insertion into membrane raft4
localizes aat1IMPPMID:18310029
localizes pma1IMPPMID:18310029
NOT GO:0006897endocytosisIMPPMID:18310029
NOT GO:0006623protein targeting to vacuoleIMPPMID:18310029
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005789endoplasmic reticulum membraneISSUniProtKB:P25340GO_REF:0000001207
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371017
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000674normal cell population growth at high temperatureCell growth assaysts1ΔNullPECO:0000201, PECO:0000102PMID:2314504832
FYPO:0001164normal growth on glucose carbon sourceCell growth assaysts1ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:23145048156
FYPO:0000963normal growth on hydroxyureaCell growth assaysts1ΔNullPMID:132096019
FYPO:0002329normal growth on pravastatinCell growth assaysts1ΔNullPECO:0000201, PECO:0000005PMID:231450482
FYPO:0000964normal growth on thiabendazoleCell growth assaysts1ΔNullPMID:13209607
FYPO:0000070resistance to amphotericin BCell growth assaysts1ΔNullPECO:0000137, PECO:0000005PMID:222528179
Cell growth assaysts1ΔNullPMID:18310029
FYPO:0000076resistance to nystatinCell growth assaysts1ΔNullPMID:183100296
FYPO:0000097sensitive to caffeineCell growth assaysts1ΔNullPMID:132096098
FYPO:0000100sensitive to chloramphenicolCell growth assaysts1ΔNullPMID:13209601
FYPO:0002640sensitive to clotrimazole105
expressivity FYPO_EXT:0000001Cell growth assaysts1ΔNullPECO:0000137, PECO:0000005PMID:22252817
FYPO:0000080sensitive to coldCell growth assaysts1ΔNullPMID:132096042
FYPO:0000104sensitive to cycloheximideCell growth assaysts1ΔNullPMID:1831002918
FYPO:0002641sensitive to micafunginCell growth assaysts1ΔNullPECO:0000137, PECO:0000005PMID:22252817116
FYPO:0000841sensitive to sodium dodecyl sulfateCell growth assaysts1ΔNullPMID:13209605
FYPO:0000112sensitive to sorbitolCell growth assaysts1ΔNullPMID:13209607
FYPO:0000113sensitive to staurosporineCell growth assaysts1-5Not specifiedPECO:0000201, PECO:0000102, PECO:0000005PMID:18992308
Cell growth assaysts1EndogenousPMID:1320960
Cell growth assaysts1ΔNullPMID:1320960
FYPO:0002328sensitive to terbinafine101
expressivity FYPO_EXT:0000001Cell growth assaysts1ΔNullPECO:0000137, PECO:0000005PMID:22252817
FYPO:0002060viable vegetative cell populationMicroscopysts1ΔNullPMID:204732893730
Microscopysts1ΔNullPECO:0000005, PECO:0000137PMID:23697806

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000121abnormal sporulationMicroscopynot recorded (unrecorded)PMID:18310029159
FYPO:0002313ergosterol absent from cellSubstance quantification evidencests1ΔNullPMID:231450481
FYPO:0000282viable cell at high temperatureCell growth assaysts1ΔNullPECO:0000201, PECO:0000102PMID:231450488
FYPO:0001491viable vegetative cellCell growth assaysts1ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:231450483600
Cell growth assaysts1ΔNullPMID:1320960
FYPO:0002177viable vegetative cell with normal cell morphology3088
penetrance FYPO_EXT:0000001Microscopysts1ΔNullPECO:0000005, PECO:0000137PMID:23697806
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
148281414826601

UTRs

Region Coordinates Reference
three_prime_UTR4826682..4826601PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01222 Pfam IPR001171 Ergosterol biosynthesis ERG4/ERG24 8 453 2
TMhelix TMHMM 150 172 959
TMhelix TMHMM 304 326 959
TMhelix TMHMM 75 97 959
TMhelix TMHMM 13 35 959
TMhelix TMHMM 118 140 959
TMhelix TMHMM 277 299 959
TMhelix TMHMM 210 229 959
TMhelix TMHMM 396 418 959
PS01018 Prosite Patterns IPR018083 Sterol reductase, conserved site 417 440 2
PS01017 Prosite Patterns IPR018083 Sterol reductase, conserved site 185 200 2
PTHR21257 HMMPANTHER 2 453 2
PTHR21257:SF31 HMMPANTHER 2 453 1

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 116.00 Da
Charge 23.00
Isoelectric point 9.05
Molecular weight 52.55 kDa
Number of residues 453
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
4498during GO:0000080PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
6600during GO:0000084PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
4090during GO:0000085PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
5625during GO:0000087PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
5697during GO:0072690PECO:0000005,
PECO:0000126
mass spectrometry evidencePMID:24763107
5338.44during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
1870.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
5.2during GO:0072690PECO:0000014,
PECO:0000005
experimental evidencePMID:23101633
0.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000014,
PECO:0000127,
PECO:0000005
experimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4569
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3393
conserved in eukaryotes only2487
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic LethalityPMID:7983142
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPAC20G4.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G4.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G4.07c BioGRID Interaction Datasets
Expression Viewer SPAC20G4.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G4.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G4.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G4.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G4.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G4.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC20G4.07c Cell Cycle Data
GEO SPAC20G4.07c GEO profiles
PInt SPAC20G4.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC20G4.07c Peptides identified in tandem mass spectrometry proteomics experiments
WikiGene2541740C-24(28) sterol reductase Sts1
EntrezGene2541740C-24(28) sterol reductase Sts1
IntEnz1.3.1.71Integrated relational Enzyme database
Rhea1.3.1.71Annotated reactions database
SPD / RIKEN44/44C10Orfeome Localization Data
UniProtKB/SwissProtP36209Delta(24(24(1)))-sterol reductase
ModBaseP36209Database of comparative protein structure models
STRINGP36209Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594742C-24(28) sterol reductase Sts1
RefSeq mRNANM_001020170972h- C-24(28) sterol reductase Sts1 (sts1), mRNA
European Nucleotide ArchiveCAA45113ENA Protein Mapping
European Nucleotide ArchiveCAA45113.1ENA Protein Mapping
European Nucleotide ArchiveCAB11256ENA Protein Mapping
European Nucleotide ArchiveCAB11256.1ENA Protein Mapping
UniParcUPI000013614FUniProt Archive

Literature for sts1

Search: Europe PMC or PubMed

Release Version: PomBase:22_45 - 08 Aug 2014