sts1 (SPAC20G4.07c)


Gene Standard Namests1 Characterisation Statuspublished
Systematic IDSPAC20G4.07c Feature Typeprotein coding
Synonymserg4 Name Descriptionstaurosporine supersensitive
ProductC-24(28) sterol reductase Sts1 Product Size453aa, 52.55 kDa
Genomic Location Chromosome I, 4828141-4826601 (1541nt); CDS:4828044-4826683 (1362nt)

Ensembl Gene Location
GO Molecular Function
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0000246delta24(24-1) sterol reductase activityISSUniProtKB:P25340GO_REF:00000011
GO Biological Process
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0030437ascospore formationIMPPMID:1831002999
GO:0071473cellular response to cation stressIMPPMID:13209609
GO:0071470cellular response to osmotic stressIMPPMID:132096044
NOT GO:0006897endocytosisIMPPMID:18310029
GO:0006696ergosterol biosynthetic processIMPPMID:2314504839
IMPPMID:18310029
GO:0090002establishment of protein localization to plasma membrane13
localizes SPBC359.03IMPPMID:18310029
GO:0071210protein insertion into membrane raft4
localizes SPBC359.03IMPPMID:18310029
localizes pma1IMPPMID:18310029
NOT GO:0006623protein targeting to vacuoleIMPPMID:18310029
GO:0042493response to drugIMPPMID:132096013
GO Cellular Component
Term IDTerm NameEvidenceWith/FromReferenceCount
GO:0005789endoplasmic reticulum membraneISSUniProtKB:P25340GO_REF:0000001207
GO:0016021integral component of membraneIEAUniProtKB-KW:KW-0812GO_REF:00000371016
Fission Yeast Phenotype Ontology

Population Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000674normal cell population growth at high temperatureCell growth assaysts1ΔNullPECO:0000201, PECO:0000102PMID:2314504823
FYPO:0001164normal cell population growth on glucose carbon sourceCell growth assaysts1ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:23145048101
FYPO:0000963normal cell population growth on hydroxyureaCell growth assaysts1ΔNullPMID:132096016
FYPO:0002329normal cell population growth on pravastatinCell growth assaysts1ΔNullPECO:0000201, PECO:0000005PMID:231450482
FYPO:0000964normal cell population growth on thiabendazoleCell growth assaysts1ΔNullPMID:13209604
FYPO:0000070resistance to amphotericin BCell growth assaysts1ΔNullPECO:0000137, PECO:0000005PMID:222528179
sts1ΔNullPMID:18310029
FYPO:0000076resistance to nystatinsts1ΔNullPMID:183100296
FYPO:0000097sensitive to caffeineCell growth assaysts1ΔNullPMID:132096095
FYPO:0000100sensitive to chloramphenicolCell growth assaysts1ΔNullPMID:13209601
FYPO:0002640sensitive to clotrimazoleCell growth assaysts1ΔNullPECO:0000137, PECO:0000005PMID:22252817105
expressivity FYPO_EXT:0000001
FYPO:0000080sensitive to coldCell growth assaysts1ΔNullPMID:132096030
FYPO:0000104sensitive to cycloheximidests1ΔNullPMID:1831002915
FYPO:0002641sensitive to micafunginCell growth assaysts1ΔNullPECO:0000137, PECO:0000005PMID:22252817105
FYPO:0000841sensitive to sodium dodecyl sulfateCell growth assaysts1ΔNullPMID:13209603
FYPO:0000112sensitive to sorbitolCell growth assaysts1ΔNullPMID:13209605
FYPO:0000113sensitive to staurosporineCell growth assaysts1ΔNullPMID:13209606
Cell growth assaysts1EndogenousPMID:1320960
Cell growth assaysts1-5Not specifiedPECO:0000201, PECO:0000102, PECO:0000005PMID:1899230
FYPO:0002328sensitive to terbinafineCell growth assaysts1ΔNullPECO:0000137, PECO:0000005PMID:22252817101
expressivity FYPO_EXT:0000001
FYPO:0002060viable vegetative cell populationMicroscopysts1ΔNullPECO:0000005, PECO:0000137PMID:236978063755
Microscopysts1ΔNullPMID:20473289

Cell Phenotype

Term IDTerm NameEvidenceAlleleExpressionConditionReferenceCount
FYPO:0000121abnormal sporulationno_namePMID:18310029110
FYPO:0002313ergosterol absent from cellSubstance quantification evidencests1ΔNullPMID:231450481
FYPO:0000282viable cell at high temperatureCell growth assaysts1ΔNullPECO:0000201, PECO:0000102PMID:2314504811
FYPO:0001491viable vegetative cellCell growth assaysts1ΔNullPMID:13209603271
Cell growth assaysts1ΔNullPECO:0000201, PECO:0000005, PECO:0000102PMID:23145048
FYPO:0002177viable vegetative cell with normal cell morphologyMicroscopysts1ΔNullPECO:0000005, PECO:0000137PMID:236978063078
penetrance FYPO_EXT:0000001
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
148281414826601

UTRs

Region Start End Reference
three_prime_UTR48266824826601PMID:21511999
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01222 Pfam IPR001171 Ergosterol biosynthesis ERG4/ERG24 8 453 2
TMhelix TMHMM 396 418 959
TMhelix TMHMM 277 299 959
TMhelix TMHMM 210 229 959
TMhelix TMHMM 118 140 959
TMhelix TMHMM 13 35 959
TMhelix TMHMM 75 97 959
TMhelix TMHMM 304 326 959
TMhelix TMHMM 150 172 959
PS01018 Prosite Patterns IPR018083 Sterol reductase, conserved site 417 440 2
PS01017 Prosite Patterns IPR018083 Sterol reductase, conserved site 185 200 2
PTHR21257:SF4 HMMPANTHER 2 453 1
PTHR21257 HMMPANTHER 2 453 2

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 116.00 Da
Charge 23.00
Isoelectric point 9.05
Molecular weight 52.55 kDa
Number of residues 453
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
1870.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
5338.44during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)DuringConditionScaleEvidenceReference
0.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population_wideexperimental evidencePMID:23101633
5.2during GO:0072690PECO:0000005,
PECO:0000014
population_wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3080
conserved in fungi4564
conserved in eukaryotes4482
conserved in metazoa3419
conserved in vertebrates3394
conserved in eukaryotes only2485
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Genetic Interactions

Source: BioGRID

Gene Product Evidence Reference
ppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic LethalityPMID:7983142
Physical Interactions

Source: BioGRID

Gene Product Evidence Reference
hhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
External References
Database Identifier Description
NBRP SPAC20G4.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G4.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G4.07c BioGRID Interaction Datasets
Expression Viewer SPAC20G4.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G4.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G4.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G4.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G4.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G4.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC20G4.07c Cell Cycle Data
GEO SPAC20G4.07c GEO profiles
PInt SPAC20G4.07c Protein-Protein Interaction Predictor (Bähler Lab)
WikiGene2541740C-24(28) sterol reductase Sts1
EntrezGene2541740C-24(28) sterol reductase Sts1
IntEnz1.3.1.71Integrated relational Enzyme database
Rhea1.3.1.71Annotated reactions database
SPD / RIKEN44/44C10Orfeome Localization Data
UniProtKB/SwissProtP36209Delta(24(24(1)))-sterol reductase
ModBaseP36209Database of comparative protein structure models
StringP36209Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594742C-24(28) sterol reductase Sts1
RefSeq mRNANM_001020170972h- C-24(28) sterol reductase Sts1 (sts1), mRNA
European Nucleotide ArchiveCAA45113ENA Protein Mapping
European Nucleotide ArchiveCAB11256ENA Protein Mapping
UniParcUPI000013614FUniProt Archive

Literature for sts1

Search: Europe PMC or PubMed

Release Version: PomBase:21_41 - 24 Feb 2014