sts1 (SPAC20G4.07c)


Gene Standard Namests1 Characterisation Statuspublished
Systematic IDSPAC20G4.07c Feature Typeprotein coding
Synonymserg4 Name Descriptionstaurosporine supersensitive
ProductC-24(28) sterol reductase Sts1 Product Size453aa, 52.55 kDa
Genomic Location Chromosome I, 4828141-4826601 (1541nt); CDS:4828044-4826683 (1362nt)

Ensembl Gene Location
GO Molecular Function
Term NameCount
delta24(24-1) sterol reductase activity1
Annotation ExtensionEvidenceWith/FromReference
GO Biological Process
Term NameCount
ascospore formation75
Annotation ExtensionEvidenceWith/FromReference
cellular response to cation stress8
Annotation ExtensionEvidenceWith/FromReference
cellular response to osmotic stress35
Annotation ExtensionEvidenceWith/FromReference
ergosterol biosynthetic process44
Annotation ExtensionEvidenceWith/FromReference
establishment of protein localization to plasma membrane20
Annotation ExtensionEvidenceWith/FromReference
protein insertion into membrane raft4
Annotation ExtensionEvidenceWith/FromReference
NOT endocytosis
Annotation ExtensionEvidenceWith/FromReference
NOT protein targeting to vacuole
Annotation ExtensionEvidenceWith/FromReference
GO Cellular Component
Term NameCount
endoplasmic reticulum membrane205
Annotation ExtensionEvidenceWith/FromReference
integral component of membrane1018
Annotation ExtensionEvidenceWith/FromReference
Fission Yeast Phenotype Ontology
Gene Deletion Viability: Viable

Population Phenotype

Term NameAlleleExpressionCount
normal cell population growth at high temperaturests1ΔNull44
normal growth on glucose carbon sourcests1ΔNull194
normal growth on hydroxyureasts1ΔNull34
normal growth on pravastatinsts1ΔNull2
normal growth on thiabendazolests1ΔNull11
resistance to amphotericin Bsts1ΔNull9
resistance to nystatinsts1ΔNull6
sensitive to caffeinests1ΔNull108
sensitive to chloramphenicolsts1ΔNull1
sensitive to clotrimazolests1ΔNull105
sensitive to coldsts1ΔNull45
sensitive to cycloheximidests1ΔNull18
sensitive to cyclosporin Asts1ΔNull6
sensitive to micafunginsts1ΔNull116
sensitive to sodium dodecyl sulfatests1ΔNull6
sensitive to sorbitolsts1ΔNull11
sensitive to staurosporinests1Endogenous9
sts1-5Not specified
sts1ΔNull
sensitive to terbinafinests1ΔNull102
viable vegetative cell populationsts1ΔNull3783

Cell Phenotype

Term NameAlleleExpressionCount
abnormal microtubule cytoskeleton morphology during mitotic interphasests1ΔNull186
abnormal sporulationunnamed (unrecorded)198
elongated multinucleate multiseptate vegetative cell, septa between nucleists1ΔNull16
ergosterol absent from cellsts1ΔNull1
viable cell at high temperaturests1ΔNull8
viable vegetative cellsts1ΔNull3615
viable vegetative cell with normal cell morphologysts1ΔNull3092
Transcript
Ensembl transcript structure with UTRs, exons and introns

Exons

Exon Start End
148281414826601

UTRs

Region Coordinates Reference
three_prime_UTR4826682..4826601PMID:21511999
exon4828044..4826683
mRNA4828141..4826601
five_prime_UTR4828141..4828045AU010943
Protein Features

Graphical View

Ensembl protein image with mapped locations of structural domains

Protein Families and Domains

Feature ID Database InterPro Description Start End Count
PF01222 Pfam IPR001171 Ergosterol biosynthesis ERG4/ERG24 8 453 2
TMhelix TMHMM 210 229 959
TMhelix TMHMM 118 140 959
TMhelix TMHMM 75 97 959
TMhelix TMHMM 277 299 959
TMhelix TMHMM 304 326 959
TMhelix TMHMM 150 172 959
TMhelix TMHMM 396 418 959
TMhelix TMHMM 13 35 959
PS01017 Prosite Patterns IPR018083 Sterol reductase, conserved site 185 200 2
PS01018 Prosite Patterns IPR018083 Sterol reductase, conserved site 417 440 2
PTHR21257:SF31 HMMPANTHER 2 453 1
PTHR21257 HMMPANTHER 2 453 2

View domain organization at Pfam

Sequence Ontology

Term IDTerm NameReferenceCount
SO:0001812transmembrane_helix465

Protein Properties

Ave. residue weight 116.00 Da
Charge 23.00
Isoelectric point 9.05
Molecular weight 52.55 kDa
Number of residues 453
Sequence
Gene Expression

Quantitative Gene Expression

Protein Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
4498during GO:0000080PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
6600during GO:0000084PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
4090during GO:0000085PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5625during GO:0000087PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5697during GO:0072690PECO:0000126,
PECO:0000005
single cellmass spectrometry evidencePMID:24763107
5338.44during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
1870.63during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633

RNA Level

Molecules/Cell (average)ExtensionConditionScaleEvidenceReference
5.2during GO:0072690PECO:0000005,
PECO:0000014
population wideexperimental evidencePMID:23101633
0.47during cell quiescence following G1 arrest due to nitrogen limitationPECO:0000005,
PECO:0000014,
PECO:0000127
population wideexperimental evidencePMID:23101633
Species Distribution
DescriptionQualifierReferenceCount
predominantly single copy (one to one)3092
conserved in fungi4604
conserved in eukaryotes4516
conserved in metazoa3432
conserved in vertebrates3407
conserved in eukaryotes only2503
Orthologs

Manually curated orthologous groups

SpeciesGeneDescriptionLinksCount

Orthologs in Compara


Physical Interactions

Source: BioGRID

View all interactions in esyN
View the HCPIN interactions in esyN

Gene Product Evidence Reference
affinity captured byhhp2serine/threonine protein kinase Hhp2 Affinity Capture-MSPMID:24055157
Genetic Interactions

Source: BioGRID

View these interactions in esyN

Gene Product Evidence Reference
synthetic lethal withppb1calcium-dependent serine/threonine protein phosphatase calcineurin A, catalytic subunit Ppb1 Synthetic LethalityPMID:7983142
External References
Database Identifier Description
NBRP SPAC20G4.07c Fission yeast strain database, National BioResource Project (Japan)
YOGY SPAC20G4.07c Retrieval of eukaryotic orthologs (Bähler Lab)
BioGrid SPAC20G4.07c BioGRID Interaction Datasets
Expression Viewer SPAC20G4.07c Cell Cycle Expression Profile (Bähler Lab)
Expression Viewer SPAC20G4.07c Meiosis/Sporulation Expression Profies (Bähler Lab)
Expression Viewer SPAC20G4.07c Pheromone response/mating expression profiles (Bähler Lab)
Expression Viewer SPAC20G4.07c Environmental stress expression profiles (Bähler Lab)
Pomb(A) SPAC20G4.07c Polyadenylation Viewer (Gullerova lab)
pombeTV SPAC20G4.07c Transcriptome Viewer (Bähler Lab)
Cyclebase SPAC20G4.07c Cell Cycle Data
GEO SPAC20G4.07c GEO profiles
PInt SPAC20G4.07c Protein-Protein Interaction Predictor (Bähler Lab)
PeptideAtlas SPAC20G4.07c Peptides identified in tandem mass spectrometry proteomics experiments
SYSGRO SPAC20G4.07c Fission yeast phenotypic data & analysis
SPD / RIKEN44/44C10Orfeome Localization Data
IntEnz1.3.1.71Integrated relational Enzyme database
Rhea1.3.1.71Annotated reactions database
UniProtKB/SwissProtP36209Delta(24(24(1)))-sterol reductase
ModBaseP36209Database of comparative protein structure models
STRINGP36209Network display of known and predicted interactions and functional associations
RefSeq PeptideNP_594742C-24(28) sterol reductase Sts1
RefSeq mRNANM_001020170972h- C-24(28) sterol reductase Sts1 (sts1), mRNA
European Nucleotide ArchiveCAA45113.1ENA Protein Mapping
European Nucleotide ArchiveCAB11256.1ENA Protein Mapping
UniParcUPI000013614FUniProt Archive

Literature for sts1

Search: Europe PMC or PubMed

Release Version: PomBase:25_49 - 02 Feb 2015